AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023713.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023713.01.T01 MTR_8g099695 97.045 440 13 0 1 440 1 440 0.0 883
MsG0480023713.01.T01 MTR_1g103020 73.232 396 105 1 43 437 32 427 0.0 623
MsG0480023713.01.T01 MTR_4g096690 68.852 427 123 1 11 437 9 425 0.0 613
MsG0480023713.01.T01 MTR_8g099715 71.322 401 64 3 40 440 3 352 0.0 568
MsG0480023713.01.T01 MTR_8g099700 74.351 308 43 3 4 309 16 289 1.69e-162 459
MsG0480023713.01.T01 MTR_2g043530 52.750 400 186 2 37 436 46 442 7.42e-150 434
MsG0480023713.01.T01 MTR_2g043530 52.750 400 186 2 37 436 46 442 2.00e-149 434
MsG0480023713.01.T01 MTR_4g124880 49.760 416 208 1 23 438 33 447 1.00e-145 424
MsG0480023713.01.T01 MTR_4g124880 55.298 302 134 1 137 438 1 301 4.66e-124 364
MsG0480023713.01.T01 MTR_1g069385 38.875 391 213 5 70 436 37 425 2.75e-82 261
MsG0480023713.01.T01 MTR_1g069385 44.000 300 162 3 141 436 5 302 1.76e-73 234
MsG0480023713.01.T01 MTR_8g099705 90.196 102 10 0 339 440 1 102 4.11e-59 191
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023713.01.T01 AT5G46290 73.604 394 104 0 46 439 37 430 0.0 627
MsG0480023713.01.T01 AT5G46290 73.724 392 103 0 46 437 37 428 0.0 626
MsG0480023713.01.T01 AT5G46290 65.263 380 77 2 58 437 49 373 0.0 511
MsG0480023713.01.T01 AT1G74960 54.354 379 172 1 60 438 120 497 3.72e-147 430
MsG0480023713.01.T01 AT1G74960 54.354 379 172 1 60 438 120 497 3.72e-147 430
MsG0480023713.01.T01 AT1G74960 54.354 379 172 1 60 438 120 497 3.72e-147 430
MsG0480023713.01.T01 AT2G04540 37.596 391 215 6 70 436 31 416 9.02e-73 236

Find 108 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACAGCAGTTCTGCTTTAT+TGG 0.161775 4:+88871446 MsG0480023713.01.T01:CDS
TACTTCTTTGTTACCTCTTC+AGG 0.244828 4:-88872166 None:intergenic
CATGGTCTTGAAGCCTTCTT+AGG 0.261630 4:-88871672 None:intergenic
ATGAAATATTGTTTAGTTGC+AGG 0.283817 4:+88870970 MsG0480023713.01.T01:CDS
ATGGGTCTTGTGTCAGTCTT+TGG 0.300758 4:+88870778 MsG0480023713.01.T01:CDS
AGACAATACATCTTGGTTAA+TGG 0.300909 4:-88872735 None:intergenic
AGGCATCAAACCTATCTATT+AGG 0.312929 4:-88870855 None:intergenic
TCAAGCTTCCCCGTCCGTTT+TGG 0.321480 4:+88870877 MsG0480023713.01.T01:CDS
TCTTCAAAAGGTTACATTGA+TGG 0.328774 4:+88870919 MsG0480023713.01.T01:CDS
GCTTTATTGGCTATAGAAAC+TGG 0.329983 4:+88871459 MsG0480023713.01.T01:CDS
AAAATAATTGCTGAATATTT+GGG 0.338704 4:+88872040 MsG0480023713.01.T01:CDS
GGCTATAGAAACTGGCCTAA+TGG 0.341363 4:+88871467 MsG0480023713.01.T01:CDS
GCAGTAGAAATGGAATAATT+TGG 0.351291 4:-88871492 None:intergenic
TTGGAGCATTCATTGCTTGC+AGG 0.356258 4:+88871625 MsG0480023713.01.T01:CDS
AAGTGGTGAAGTAGATATTA+TGG 0.364011 4:+88871563 MsG0480023713.01.T01:CDS
GCTATAGAAACTGGCCTAAT+GGG 0.366775 4:+88871468 MsG0480023713.01.T01:CDS
GCCAGTTGTTATTGCTTTGA+CGG 0.381184 4:-88872699 None:intergenic
TATTGGTGCTGCTGGTGCTT+TGG 0.382432 4:+88872664 MsG0480023713.01.T01:CDS
TGGAATAATTTGGTCCCATT+AGG 0.388383 4:-88871482 None:intergenic
TCATTCTAAGCCCTTCATAC+TGG 0.394389 4:-88869988 None:intergenic
CAGATGGACAAAACAAGAAT+AGG 0.412901 4:+88871309 MsG0480023713.01.T01:intron
TTTGTCCTTGGTGAAGGCTC+TGG 0.413751 4:+88871714 MsG0480023713.01.T01:CDS
TCACTTATACCTTCTGGTGT+TGG 0.427107 4:+88871606 MsG0480023713.01.T01:CDS
GTGGGATAAGCCTAATAGAT+AGG 0.427864 4:+88870845 MsG0480023713.01.T01:CDS
TGGTGAAGGCTCTGGTGTAC+TGG 0.437806 4:+88871722 MsG0480023713.01.T01:CDS
GCACTTGAAGATGCTAATCT+TGG 0.441793 4:+88871000 MsG0480023713.01.T01:CDS
GGTCATTGTATTGGTGCTGC+TGG 0.443878 4:+88872656 MsG0480023713.01.T01:CDS
ATGATGAGCATCACATGTTA+TGG 0.444772 4:-88872068 None:intergenic
AATAAAGCAGAACTGCTGTT+AGG 0.448303 4:-88871444 None:intergenic
CTCTCACTCGAAAGGGTCAT+AGG 0.461629 4:+88871388 MsG0480023713.01.T01:CDS
CACCGTGATGGCTTTGTCCT+TGG 0.463528 4:+88871702 MsG0480023713.01.T01:CDS
GATGGCTTTGTCCTTGGTGA+AGG 0.465037 4:+88871708 MsG0480023713.01.T01:CDS
CATCAGAATTGAAAATGAAC+GGG 0.470500 4:+88872526 MsG0480023713.01.T01:CDS
ACCGTCAAAGCAATAACAAC+TGG 0.472479 4:+88872698 MsG0480023713.01.T01:CDS
GAAGCTTCACTTATACCTTC+TGG 0.473210 4:+88871600 MsG0480023713.01.T01:CDS
ATAGGAGTTCTAGTAGGATC+AGG 0.478687 4:+88871327 MsG0480023713.01.T01:CDS
ATCAACAGCATTGTTAAAAG+TGG 0.481436 4:-88871364 None:intergenic
GAGAGTAGTAATAACAGGAA+TGG 0.481577 4:+88870759 MsG0480023713.01.T01:CDS
GTATCTTTGGTCCAGTATGA+AGG 0.482698 4:+88869977 MsG0480023713.01.T01:CDS
TCAATGATTGGTCATTGTAT+TGG 0.483833 4:+88872647 MsG0480023713.01.T01:CDS
ATTGAAAATGAACGGGACTA+AGG 0.484775 4:+88872533 MsG0480023713.01.T01:CDS
CATGCCACATCCACGGTAGC+TGG 0.488866 4:+88872459 MsG0480023713.01.T01:CDS
AGAGTAGTAATAACAGGAAT+GGG 0.489390 4:+88870760 MsG0480023713.01.T01:CDS
AACAAGGGTTTAGAAGATGC+TGG 0.493576 4:+88872136 MsG0480023713.01.T01:CDS
ATGACTGATCCAAGGTCTGA+TGG 0.494024 4:+88872091 MsG0480023713.01.T01:CDS
ATTGTTTAGTTGCAGGAAAG+AGG 0.494720 4:+88870977 MsG0480023713.01.T01:CDS
ACAAGGGTTTAGAAGATGCT+GGG 0.495000 4:+88872137 MsG0480023713.01.T01:CDS
CGCGTATCTGACCACCAAAA+CGG 0.495054 4:-88870891 None:intergenic
ATACGCGATTTCTCTTCAAA+AGG 0.496139 4:+88870907 MsG0480023713.01.T01:CDS
GCGGTCAAGACAATACATCT+TGG 0.496527 4:-88872742 None:intergenic
AATACATCTTGGTTAATGGT+TGG 0.497383 4:-88872731 None:intergenic
AGTTCTAGTAGGATCAGGAA+TGG 0.500705 4:+88871332 MsG0480023713.01.T01:CDS
AGAAGGTATAAGTGAAGCTT+CGG 0.506743 4:-88871598 None:intergenic
TGTTGCACAAGCAGTAGAAA+TGG 0.509995 4:-88871502 None:intergenic
AAGCTTCTAGAGGGAGAAAG+TGG 0.510159 4:+88870826 MsG0480023713.01.T01:CDS
GTCACCAGCTACCGTGGATG+TGG 0.515201 4:-88872463 None:intergenic
CAAGAAATGCAAAACAATCA+AGG 0.518811 4:+88870684 MsG0480023713.01.T01:CDS
ATCTGACCACCAAAACGGAC+GGG 0.522249 4:-88870886 None:intergenic
CTTAGAGAGTGCAACAAAGA+GGG 0.523554 4:+88872012 MsG0480023713.01.T01:CDS
ATCCACGGTAGCTGGTGACT+TGG 0.524496 4:+88872467 MsG0480023713.01.T01:CDS
TGTTTCATCTTGCATAAACA+AGG 0.525515 4:+88872120 MsG0480023713.01.T01:CDS
GATCCAAGGTCTGATGGACT+TGG 0.526084 4:+88872097 MsG0480023713.01.T01:CDS
TGGACCAAAGATACCTCAAA+AGG 0.527943 4:-88869968 None:intergenic
GAAGTAGATATTATGGTGGT+TGG 0.533930 4:+88871570 MsG0480023713.01.T01:CDS
GAGGAATGAAGAGCCTAAGA+AGG 0.534878 4:+88871659 MsG0480023713.01.T01:CDS
ACAAGAATAGGAGTTCTAGT+AGG 0.539630 4:+88871321 MsG0480023713.01.T01:CDS
GTTGATGCTCTCACTCGAAA+GGG 0.544680 4:+88871381 MsG0480023713.01.T01:CDS
TTGTTTAGTTGCAGGAAAGA+GGG 0.545243 4:+88870978 MsG0480023713.01.T01:CDS
TGGAGCATTCATTGCTTGCA+GGG 0.554208 4:+88871626 MsG0480023713.01.T01:CDS
ACATCAGAATTGAAAATGAA+CGG 0.560317 4:+88872525 MsG0480023713.01.T01:CDS
GGTAGCTGGTGACTTGGCTG+AGG 0.563821 4:+88872473 MsG0480023713.01.T01:CDS
CCATCACGGTGTTTGTCCCA+TGG 0.565247 4:-88871690 None:intergenic
GTTCTAGTAGGATCAGGAAT+GGG 0.565575 4:+88871333 MsG0480023713.01.T01:CDS
ATGCAAAACAATCAAGGTCA+AGG 0.567045 4:+88870690 MsG0480023713.01.T01:CDS
TGTTGATGCTCTCACTCGAA+AGG 0.568540 4:+88871380 MsG0480023713.01.T01:CDS
ACTGCTGTTAGGAATGGTGT+AGG 0.571087 4:-88871433 None:intergenic
AGCAGAACTGCTGTTAGGAA+TGG 0.571244 4:-88871439 None:intergenic
TATCTTTGGTCCAGTATGAA+GGG 0.577661 4:+88869978 MsG0480023713.01.T01:CDS
ACATTGATGGCAAGACTGAT+CGG 0.587450 4:+88870932 MsG0480023713.01.T01:CDS
GCTTAGAGAGTGCAACAAAG+AGG 0.590788 4:+88872011 MsG0480023713.01.T01:CDS
CTTGCAGGGCGTTGTCTCAG+AGG 0.590883 4:+88871640 MsG0480023713.01.T01:CDS
TGCTGGGGTTTCTCCTGAAG+AGG 0.591348 4:+88872153 MsG0480023713.01.T01:CDS
CTAAGAAGGCTTCAAGACCA+TGG 0.592322 4:+88871673 MsG0480023713.01.T01:CDS
TCAAAGCAATAACAACTGGC+TGG 0.592693 4:+88872702 MsG0480023713.01.T01:CDS
TCTGACCACCAAAACGGACG+GGG 0.595025 4:-88870885 None:intergenic
TAAGAAGGCTTCAAGACCAT+GGG 0.598820 4:+88871674 MsG0480023713.01.T01:CDS
AAAACTAACATGGCATGCAA+TGG 0.600866 4:+88869929 None:intergenic
GCTTCTCATCACATTAGAAG+TGG 0.604035 4:+88871546 MsG0480023713.01.T01:CDS
GTTTCATCTTGCATAAACAA+GGG 0.606872 4:+88872121 MsG0480023713.01.T01:CDS
ATGAATGCTCCAACACCAGA+AGG 0.607630 4:-88871615 None:intergenic
CACCAAGGACAAAGCCATCA+CGG 0.608128 4:-88871704 None:intergenic
TATCTGACCACCAAAACGGA+CGG 0.610935 4:-88870887 None:intergenic
CTGCTGTTAGGAATGGTGTA+GGG 0.611344 4:-88871432 None:intergenic
CAAGGGTTTAGAAGATGCTG+GGG 0.612194 4:+88872138 MsG0480023713.01.T01:CDS
ACACCAAGTCCATCAGACCT+TGG 0.614275 4:-88872100 None:intergenic
AGCCAAGTCACCAGCTACCG+TGG 0.614633 4:-88872469 None:intergenic
CTCATCATATGACTGATCCA+AGG 0.618797 4:+88872083 MsG0480023713.01.T01:CDS
AAAACGGACGGGGAAGCTTG+AGG 0.623712 4:-88870875 None:intergenic
CCATGGGACAAACACCGTGA+TGG 0.625136 4:+88871690 MsG0480023713.01.T01:CDS
TTAGAGAGTGCAACAAAGAG+GGG 0.630279 4:+88872013 MsG0480023713.01.T01:CDS
GTAGATATTATGGTGGTTGG+TGG 0.636144 4:+88871573 MsG0480023713.01.T01:CDS
GAATGCTCATGCCACATCCA+CGG 0.642543 4:+88872452 MsG0480023713.01.T01:CDS
CTAGTAGGATCAGGAATGGG+AGG 0.653490 4:+88871336 MsG0480023713.01.T01:CDS
AGCTTCTAGAGGGAGAAAGT+GGG 0.654437 4:+88870827 MsG0480023713.01.T01:CDS
ATAATTGCTGAATATTTGGG+TGG 0.660819 4:+88872043 MsG0480023713.01.T01:CDS
TGGTGAAGTAGATATTATGG+TGG 0.671392 4:+88871566 MsG0480023713.01.T01:CDS
AATATTTCATGCAATCATCG+AGG 0.737038 4:-88870957 None:intergenic
GTACACCAGAGCCTTCACCA+AGG 0.794543 4:-88871719 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATTAATACTAAAAAAAA+CGG - Chr4:88870412-88870431 None:intergenic 10.0%
!! AAAAATAATTGCTGAATATT+TGG + Chr4:88872039-88872058 MsG0480023713.01.T01:CDS 15.0%
!! AAAATAATTGCTGAATATTT+GGG + Chr4:88872040-88872059 MsG0480023713.01.T01:CDS 15.0%
!! AATTAATACTAAAAAAAACG+GGG - Chr4:88870410-88870429 None:intergenic 15.0%
!! AGTAAAAAAAAAGAAAAAGA+AGG - Chr4:88870082-88870101 None:intergenic 15.0%
!! GAATTAATACTAAAAAAAAC+GGG - Chr4:88870411-88870430 None:intergenic 15.0%
!! TGAATAAAATGAATAAAATG+AGG - Chr4:88871905-88871924 None:intergenic 15.0%
!! AAAGAAGTAATCATTAACAT+CGG + Chr4:88872180-88872199 MsG0480023713.01.T01:intron 20.0%
!! ATATCAATGAGATAAGATAA+TGG + Chr4:88871985-88872004 MsG0480023713.01.T01:intron 20.0%
!! ATCTTACCTTTATTGTTAAT+TGG + Chr4:88870633-88870652 MsG0480023713.01.T01:intron 20.0%
!! ATTAAGAAAATAGATTCACT+TGG + Chr4:88870270-88870289 MsG0480023713.01.T01:intron 20.0%
!! ATTTCAAACATGTACTTAAT+CGG + Chr4:88872353-88872372 MsG0480023713.01.T01:intron 20.0%
!! CTATATTAATATCGATATCT+CGG + Chr4:88870533-88870552 MsG0480023713.01.T01:intron 20.0%
!! TATATTAATATCGATATCTC+GGG + Chr4:88870534-88870553 MsG0480023713.01.T01:intron 20.0%
!! TTAACTTAATAAGTTCTGTT+TGG + Chr4:88870212-88870231 MsG0480023713.01.T01:intron 20.0%
!! TTATATTGACAATATGTTCA+AGG - Chr4:88870574-88870593 None:intergenic 20.0%
!!! ACAATTTATGGATTTTCATT+GGG + Chr4:88870359-88870378 MsG0480023713.01.T01:intron 20.0%
!!! AGTATTAAGAGTACAATTTA+TGG + Chr4:88870347-88870366 MsG0480023713.01.T01:intron 20.0%
!!! CAAATATTTTCTCTTTTTGA+CGG + Chr4:88870306-88870325 MsG0480023713.01.T01:intron 20.0%
!!! CTAAATCTTTTAAACATGTT+GGG - Chr4:88870441-88870460 None:intergenic 20.0%
!!! TAAAATTTGAAATAACTGAG+AGG + Chr4:88871195-88871214 MsG0480023713.01.T01:intron 20.0%
!!! TACAATTTATGGATTTTCAT+TGG + Chr4:88870358-88870377 MsG0480023713.01.T01:intron 20.0%
!!! TATCCTATTTTTTTAACAGA+TGG + Chr4:88871293-88871312 MsG0480023713.01.T01:intron 20.0%
!!! TCTAAATCTTTTAAACATGT+TGG - Chr4:88870442-88870461 None:intergenic 20.0%
!!! TCTCAGTTATTTCAAATTTT+AGG - Chr4:88871196-88871215 None:intergenic 20.0%
! AAAAAGAGAGTAGTAATAAC+AGG + Chr4:88870754-88870773 MsG0480023713.01.T01:CDS 25.0%
! AACATCAAGAATGTAAAACT+TGG - Chr4:88870170-88870189 None:intergenic 25.0%
! AAGAAGTAATCATTAACATC+GGG + Chr4:88872181-88872200 MsG0480023713.01.T01:intron 25.0%
! AAGATCAATAAGATGTTGTT+GGG - Chr4:88872593-88872612 None:intergenic 25.0%
! ACATCAGAATTGAAAATGAA+CGG + Chr4:88872525-88872544 MsG0480023713.01.T01:CDS 25.0%
! AGCTTAGAAATTAACCAATT+AGG - Chr4:88872415-88872434 None:intergenic 25.0%
! ATGAAATATTGTTTAGTTGC+AGG + Chr4:88870970-88870989 MsG0480023713.01.T01:CDS 25.0%
! CAACATGTATTTACATACTA+CGG - Chr4:88871161-88871180 None:intergenic 25.0%
! TCAACTCCAATTAACAATAA+AGG - Chr4:88870642-88870661 None:intergenic 25.0%
! TGTCCATCTGTTAAAAAAAT+AGG - Chr4:88871299-88871318 None:intergenic 25.0%
! TTCTTAAAGTTTCCTACTTT+GGG + Chr4:88871058-88871077 MsG0480023713.01.T01:intron 25.0%
! TTTCTTAAAGTTTCCTACTT+TGG + Chr4:88871057-88871076 MsG0480023713.01.T01:intron 25.0%
!! AAACTTGGATATTTAAAGCA+TGG - Chr4:88870155-88870174 None:intergenic 25.0%
!! AGGTAAGATAAACATGTTTT+AGG - Chr4:88870622-88870641 None:intergenic 25.0%
!! GAAGCTAAAATGTATCTTTT+CGG - Chr4:88870049-88870068 None:intergenic 25.0%
!! GTATCATTATTCTTTTGAAC+CGG + Chr4:88871246-88871265 MsG0480023713.01.T01:intron 25.0%
!! TGCAATCAAACAAGTTTTTA+AGG + Chr4:88872500-88872519 MsG0480023713.01.T01:CDS 25.0%
!!! AAAAGATACATTTTAGCTTC+AGG + Chr4:88870049-88870068 MsG0480023713.01.T01:CDS 25.0%
!!! ACTTTTGACTTACATTGTTA+AGG - Chr4:88871035-88871054 None:intergenic 25.0%
!!! ATCAAATTTCTGAAACAAGA+TGG - Chr4:88871764-88871783 None:intergenic 25.0%
!!! GTTATTACTACTCTCTTTTT+TGG - Chr4:88870754-88870773 None:intergenic 25.0%
!!! TAAAGTTGATGAATCCTAAT+TGG + Chr4:88872398-88872417 MsG0480023713.01.T01:intron 25.0%
!!! TCTTTTTTTCAGTCAATGAT+TGG + Chr4:88872635-88872654 MsG0480023713.01.T01:intron 25.0%
!!! TGTTTTAGGTAACATTTCTT+TGG - Chr4:88870608-88870627 None:intergenic 25.0%
AAGTGGTGAAGTAGATATTA+TGG + Chr4:88871563-88871582 MsG0480023713.01.T01:CDS 30.0%
AATACATCTTGGTTAATGGT+TGG - Chr4:88872734-88872753 None:intergenic 30.0%
AATATTTCATGCAATCATCG+AGG - Chr4:88870960-88870979 None:intergenic 30.0%
AGACAATACATCTTGGTTAA+TGG - Chr4:88872738-88872757 None:intergenic 30.0%
AGAGTAGTAATAACAGGAAT+GGG + Chr4:88870760-88870779 MsG0480023713.01.T01:CDS 30.0%
ATAATTGCTGAATATTTGGG+TGG + Chr4:88872043-88872062 MsG0480023713.01.T01:CDS 30.0%
ATCAACAGCATTGTTAAAAG+TGG - Chr4:88871367-88871386 None:intergenic 30.0%
CAAGAAATGCAAAACAATCA+AGG + Chr4:88870684-88870703 MsG0480023713.01.T01:CDS 30.0%
CAAGATCAATAAGATGTTGT+TGG - Chr4:88872594-88872613 None:intergenic 30.0%
CATCAGAATTGAAAATGAAC+GGG + Chr4:88872526-88872545 MsG0480023713.01.T01:CDS 30.0%
CTTAGTACATGTTTAGATTG+CGG + Chr4:88872217-88872236 MsG0480023713.01.T01:intron 30.0%
GCAGTAGAAATGGAATAATT+TGG - Chr4:88871495-88871514 None:intergenic 30.0%
GCTTAAATTCAAAACAGAGA+AGG - Chr4:88870481-88870500 None:intergenic 30.0%
GGTTAATTTCTAAGCTGTAA+AGG + Chr4:88872419-88872438 MsG0480023713.01.T01:intron 30.0%
GTTTCATCTTGCATAAACAA+GGG + Chr4:88872121-88872140 MsG0480023713.01.T01:CDS 30.0%
TACTGTTAGAATAGCAATTC+CGG - Chr4:88871268-88871287 None:intergenic 30.0%
TCACATGCATAACAAATCAT+GGG - Chr4:88871841-88871860 None:intergenic 30.0%
TCTTCAAAAGGTTACATTGA+TGG + Chr4:88870919-88870938 MsG0480023713.01.T01:CDS 30.0%
TGTTTCATCTTGCATAAACA+AGG + Chr4:88872120-88872139 MsG0480023713.01.T01:CDS 30.0%
TTCACATGCATAACAAATCA+TGG - Chr4:88871842-88871861 None:intergenic 30.0%
! AATTTGAGAGTGTAACTCAT+TGG - Chr4:88870241-88870260 None:intergenic 30.0%
! ATTTTACAACAAGCTTCTAG+AGG + Chr4:88870816-88870835 MsG0480023713.01.T01:CDS 30.0%
! TCAATGATTGGTCATTGTAT+TGG + Chr4:88872647-88872666 MsG0480023713.01.T01:CDS 30.0%
! TTTGGATCTTTCTCTCTTTT+AGG - Chr4:88870736-88870755 None:intergenic 30.0%
! TTTTACAACAAGCTTCTAGA+GGG + Chr4:88870817-88870836 MsG0480023713.01.T01:CDS 30.0%
! TTTTGGCAAAAACTGCATTT+TGG + Chr4:88872272-88872291 MsG0480023713.01.T01:intron 30.0%
!! ATTTTCTCTTTTTGACGGAT+AGG + Chr4:88870311-88870330 MsG0480023713.01.T01:intron 30.0%
!! CTTGATTGTTTTGCATTTCT+TGG - Chr4:88870686-88870705 None:intergenic 30.0%
AATAAAGCAGAACTGCTGTT+AGG - Chr4:88871447-88871466 None:intergenic 35.0%
AGGCATCAAACCTATCTATT+AGG - Chr4:88870858-88870877 None:intergenic 35.0%
ATACGCGATTTCTCTTCAAA+AGG + Chr4:88870907-88870926 MsG0480023713.01.T01:CDS 35.0%
ATAGCTACCTTTGAGTACAA+CGG + Chr4:88871097-88871116 MsG0480023713.01.T01:intron 35.0%
ATGATGAGCATCACATGTTA+TGG - Chr4:88872071-88872090 None:intergenic 35.0%
ATGCAAAACAATCAAGGTCA+AGG + Chr4:88870690-88870709 MsG0480023713.01.T01:CDS 35.0%
ATGGTGTAGGGAATGAAAAA+TGG - Chr4:88871423-88871442 None:intergenic 35.0%
ATTGAAAATGAACGGGACTA+AGG + Chr4:88872533-88872552 MsG0480023713.01.T01:CDS 35.0%
ATTGTTTAGTTGCAGGAAAG+AGG + Chr4:88870977-88870996 MsG0480023713.01.T01:CDS 35.0%
CAGATGGACAAAACAAGAAT+AGG + Chr4:88871309-88871328 MsG0480023713.01.T01:intron 35.0%
GAAGTAGATATTATGGTGGT+TGG + Chr4:88871570-88871589 MsG0480023713.01.T01:CDS 35.0%
GAGAGTAGTAATAACAGGAA+TGG + Chr4:88870759-88870778 MsG0480023713.01.T01:CDS 35.0%
TAACAGCAGTTCTGCTTTAT+TGG + Chr4:88871446-88871465 MsG0480023713.01.T01:CDS 35.0%
TACTTCTTTGTTACCTCTTC+AGG - Chr4:88872169-88872188 None:intergenic 35.0%
TATCTTTGGTCCAGTATGAA+GGG + Chr4:88869978-88869997 MsG0480023713.01.T01:CDS 35.0%
TCAAAGGTAGCTATAGTTTG+TGG - Chr4:88871091-88871110 None:intergenic 35.0%
TCTAACTGAGCTACAATACA+AGG - Chr4:88870111-88870130 None:intergenic 35.0%
TGGAATAATTTGGTCCCATT+AGG - Chr4:88871485-88871504 None:intergenic 35.0%
TGGTGAAGTAGATATTATGG+TGG + Chr4:88871566-88871585 MsG0480023713.01.T01:CDS 35.0%
TTGTTTAGTTGCAGGAAAGA+GGG + Chr4:88870978-88870997 MsG0480023713.01.T01:CDS 35.0%
! ACAAGAATAGGAGTTCTAGT+AGG + Chr4:88871321-88871340 MsG0480023713.01.T01:CDS 35.0%
! AGAAGGTATAAGTGAAGCTT+CGG - Chr4:88871601-88871620 None:intergenic 35.0%
! ATATCTCGGGTTATTAGCTT+AGG + Chr4:88870547-88870566 MsG0480023713.01.T01:intron 35.0%
! CACGGTGTAACATGATATTT+TGG + Chr4:88872255-88872274 MsG0480023713.01.T01:intron 35.0%
! GCTTTATTGGCTATAGAAAC+TGG + Chr4:88871459-88871478 MsG0480023713.01.T01:CDS 35.0%
! GGCGCATATTTACTGATTAA+GGG + Chr4:88871118-88871137 MsG0480023713.01.T01:intron 35.0%
! GTTTAGTACATGTCCTTTTG+AGG + Chr4:88869955-88869974 MsG0480023713.01.T01:CDS 35.0%
! TATGGATTTTCATTGGGCAT+CGG + Chr4:88870365-88870384 MsG0480023713.01.T01:intron 35.0%
!!! ATGTCCTTTTGAGGTATCTT+TGG + Chr4:88869964-88869983 MsG0480023713.01.T01:CDS 35.0%
AAAATGAGGCTATGAGCAGT+TGG - Chr4:88871891-88871910 None:intergenic 40.0%
AACAAGGGTTTAGAAGATGC+TGG + Chr4:88872136-88872155 MsG0480023713.01.T01:CDS 40.0%
ACAAGGGTTTAGAAGATGCT+GGG + Chr4:88872137-88872156 MsG0480023713.01.T01:CDS 40.0%
ACCGTCAAAGCAATAACAAC+TGG + Chr4:88872698-88872717 MsG0480023713.01.T01:CDS 40.0%
ATGTTTAGATTGCGGTGAGT+TGG + Chr4:88872225-88872244 MsG0480023713.01.T01:intron 40.0%
CACAGTGTTTGAATTGCATC+AGG - Chr4:88871954-88871973 None:intergenic 40.0%
CTCATCATATGACTGATCCA+AGG + Chr4:88872083-88872102 MsG0480023713.01.T01:CDS 40.0%
GAAGCTTCACTTATACCTTC+TGG + Chr4:88871600-88871619 MsG0480023713.01.T01:CDS 40.0%
GCACTTGAAGATGCTAATCT+TGG + Chr4:88871000-88871019 MsG0480023713.01.T01:CDS 40.0%
GCTTCTCATCACATTAGAAG+TGG + Chr4:88871546-88871565 MsG0480023713.01.T01:CDS 40.0%
GTAGATATTATGGTGGTTGG+TGG + Chr4:88871573-88871592 MsG0480023713.01.T01:CDS 40.0%
GTATCTTTGGTCCAGTATGA+AGG + Chr4:88869977-88869996 MsG0480023713.01.T01:CDS 40.0%
GTGGGATAAGCCTAATAGAT+AGG + Chr4:88870845-88870864 MsG0480023713.01.T01:CDS 40.0%
GTTCTAGTAGGATCAGGAAT+GGG + Chr4:88871333-88871352 MsG0480023713.01.T01:CDS 40.0%
TAAGAAGGCTTCAAGACCAT+GGG + Chr4:88871674-88871693 MsG0480023713.01.T01:CDS 40.0%
TCAAAGCAATAACAACTGGC+TGG + Chr4:88872702-88872721 MsG0480023713.01.T01:CDS 40.0%
TCACTTATACCTTCTGGTGT+TGG + Chr4:88871606-88871625 MsG0480023713.01.T01:CDS 40.0%
TCATTCTAAGCCCTTCATAC+TGG - Chr4:88869991-88870010 None:intergenic 40.0%
TGGACCAAAGATACCTCAAA+AGG - Chr4:88869971-88869990 None:intergenic 40.0%
TGTGGTATCAATCCCAAAGT+AGG - Chr4:88871073-88871092 None:intergenic 40.0%
TGTTGCACAAGCAGTAGAAA+TGG - Chr4:88871505-88871524 None:intergenic 40.0%
! ACATTGATGGCAAGACTGAT+CGG + Chr4:88870932-88870951 MsG0480023713.01.T01:CDS 40.0%
! AGTTCTAGTAGGATCAGGAA+TGG + Chr4:88871332-88871351 MsG0480023713.01.T01:CDS 40.0%
! ATAGGAGTTCTAGTAGGATC+AGG + Chr4:88871327-88871346 MsG0480023713.01.T01:CDS 40.0%
! CGGCGCATATTTACTGATTA+AGG + Chr4:88871117-88871136 MsG0480023713.01.T01:intron 40.0%
! CTTAGAGAGTGCAACAAAGA+GGG + Chr4:88872012-88872031 MsG0480023713.01.T01:CDS 40.0%
! GCCAGTTGTTATTGCTTTGA+CGG - Chr4:88872702-88872721 None:intergenic 40.0%
! GCTATAGAAACTGGCCTAAT+GGG + Chr4:88871468-88871487 MsG0480023713.01.T01:CDS 40.0%
! TTAGAGAGTGCAACAAAGAG+GGG + Chr4:88872013-88872032 MsG0480023713.01.T01:CDS 40.0%
!! GCATCGGTTTACATGAACAA+TGG + Chr4:88870381-88870400 MsG0480023713.01.T01:intron 40.0%
ACTGCTGTTAGGAATGGTGT+AGG - Chr4:88871436-88871455 None:intergenic 45.0%
AGCAGAACTGCTGTTAGGAA+TGG - Chr4:88871442-88871461 None:intergenic 45.0%
ATATGCGCCGTTGTACTCAA+AGG - Chr4:88871107-88871126 None:intergenic 45.0%
ATGAATGCTCCAACACCAGA+AGG - Chr4:88871618-88871637 None:intergenic 45.0%
ATGACTGATCCAAGGTCTGA+TGG + Chr4:88872091-88872110 MsG0480023713.01.T01:CDS 45.0%
CAAGGGTTTAGAAGATGCTG+GGG + Chr4:88872138-88872157 MsG0480023713.01.T01:CDS 45.0%
CTAAGAAGGCTTCAAGACCA+TGG + Chr4:88871673-88871692 MsG0480023713.01.T01:CDS 45.0%
CTGCTGTTAGGAATGGTGTA+GGG - Chr4:88871435-88871454 None:intergenic 45.0%
GAGGAATGAAGAGCCTAAGA+AGG + Chr4:88871659-88871678 MsG0480023713.01.T01:CDS 45.0%
GTTGATGCTCTCACTCGAAA+GGG + Chr4:88871381-88871400 MsG0480023713.01.T01:CDS 45.0%
TATCTGACCACCAAAACGGA+CGG - Chr4:88870890-88870909 None:intergenic 45.0%
TGGCTGTGATGCAAGTAGAA+AGG - Chr4:88870666-88870685 None:intergenic 45.0%
TGTTGATGCTCTCACTCGAA+AGG + Chr4:88871380-88871399 MsG0480023713.01.T01:CDS 45.0%
! AAGCTTCTAGAGGGAGAAAG+TGG + Chr4:88870826-88870845 MsG0480023713.01.T01:CDS 45.0%
! AGCTTCTAGAGGGAGAAAGT+GGG + Chr4:88870827-88870846 MsG0480023713.01.T01:CDS 45.0%
! ATGGGTCTTGTGTCAGTCTT+TGG + Chr4:88870778-88870797 MsG0480023713.01.T01:CDS 45.0%
! CATGGTCTTGAAGCCTTCTT+AGG - Chr4:88871675-88871694 None:intergenic 45.0%
! GCTTAGAGAGTGCAACAAAG+AGG + Chr4:88872011-88872030 MsG0480023713.01.T01:CDS 45.0%
! GGCTATAGAAACTGGCCTAA+TGG + Chr4:88871467-88871486 MsG0480023713.01.T01:CDS 45.0%
! TGGAGCATTCATTGCTTGCA+GGG + Chr4:88871626-88871645 MsG0480023713.01.T01:CDS 45.0%
! TTGGAGCATTCATTGCTTGC+AGG + Chr4:88871625-88871644 MsG0480023713.01.T01:CDS 45.0%
!! CTTTTAGGAGCTGCAACAGT+TGG - Chr4:88870721-88870740 None:intergenic 45.0%
ACACCAAGTCCATCAGACCT+TGG - Chr4:88872103-88872122 None:intergenic 50.0%
ATCTGACCACCAAAACGGAC+GGG - Chr4:88870889-88870908 None:intergenic 50.0%
CACCAAGGACAAAGCCATCA+CGG - Chr4:88871707-88871726 None:intergenic 50.0%
CGCGTATCTGACCACCAAAA+CGG - Chr4:88870894-88870913 None:intergenic 50.0%
CTAGTAGGATCAGGAATGGG+AGG + Chr4:88871336-88871355 MsG0480023713.01.T01:CDS 50.0%
CTCTCACTCGAAAGGGTCAT+AGG + Chr4:88871388-88871407 MsG0480023713.01.T01:CDS 50.0%
GAATGCTCATGCCACATCCA+CGG + Chr4:88872452-88872471 MsG0480023713.01.T01:CDS 50.0%
TTTGTCCTTGGTGAAGGCTC+TGG + Chr4:88871714-88871733 MsG0480023713.01.T01:CDS 50.0%
! CGGTGAGTTGGACAGAATCA+CGG + Chr4:88872237-88872256 MsG0480023713.01.T01:intron 50.0%
!! GATCCAAGGTCTGATGGACT+TGG + Chr4:88872097-88872116 MsG0480023713.01.T01:CDS 50.0%
!! GATGGCTTTGTCCTTGGTGA+AGG + Chr4:88871708-88871727 MsG0480023713.01.T01:CDS 50.0%
!! GGTCATTGTATTGGTGCTGC+TGG + Chr4:88872656-88872675 MsG0480023713.01.T01:CDS 50.0%
!! TATTGGTGCTGCTGGTGCTT+TGG + Chr4:88872664-88872683 MsG0480023713.01.T01:CDS 50.0%
AAAACGGACGGGGAAGCTTG+AGG - Chr4:88870878-88870897 None:intergenic 55.0%
ATCCACGGTAGCTGGTGACT+TGG + Chr4:88872467-88872486 MsG0480023713.01.T01:CDS 55.0%
CCATCACGGTGTTTGTCCCA+TGG - Chr4:88871693-88871712 None:intergenic 55.0%
CCATGGGACAAACACCGTGA+TGG + Chr4:88871690-88871709 MsG0480023713.01.T01:CDS 55.0%
GTACACCAGAGCCTTCACCA+AGG - Chr4:88871722-88871741 None:intergenic 55.0%
TCTGACCACCAAAACGGACG+GGG - Chr4:88870888-88870907 None:intergenic 55.0%
TGCTGGGGTTTCTCCTGAAG+AGG + Chr4:88872153-88872172 MsG0480023713.01.T01:CDS 55.0%
! TCAAGCTTCCCCGTCCGTTT+TGG + Chr4:88870877-88870896 MsG0480023713.01.T01:CDS 55.0%
! TGGTGAAGGCTCTGGTGTAC+TGG + Chr4:88871722-88871741 MsG0480023713.01.T01:CDS 55.0%
!! CACCGTGATGGCTTTGTCCT+TGG + Chr4:88871702-88871721 MsG0480023713.01.T01:CDS 55.0%
AGCCAAGTCACCAGCTACCG+TGG - Chr4:88872472-88872491 None:intergenic 60.0%
CATGCCACATCCACGGTAGC+TGG + Chr4:88872459-88872478 MsG0480023713.01.T01:CDS 60.0%
GTCACCAGCTACCGTGGATG+TGG - Chr4:88872466-88872485 None:intergenic 60.0%
! AGCTTCCCCGTCCGTTTTGG+TGG + Chr4:88870880-88870899 MsG0480023713.01.T01:CDS 60.0%
! GGTAGCTGGTGACTTGGCTG+AGG + Chr4:88872473-88872492 MsG0480023713.01.T01:CDS 60.0%
!! CTTGCAGGGCGTTGTCTCAG+AGG + Chr4:88871640-88871659 MsG0480023713.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 88869938 88872760 88869938 ID=MsG0480023713.01;Name=MsG0480023713.01
Chr4 mRNA 88869938 88872760 88869938 ID=MsG0480023713.01.T01;Parent=MsG0480023713.01;Name=MsG0480023713.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|0.8|0.66|6|0|441
Chr4 exon 88869938 88870070 88869938 ID=MsG0480023713.01.T01:exon:13010;Parent=MsG0480023713.01.T01
Chr4 exon 88870683 88871041 88870683 ID=MsG0480023713.01.T01:exon:13011;Parent=MsG0480023713.01.T01
Chr4 exon 88871312 88871743 88871312 ID=MsG0480023713.01.T01:exon:13012;Parent=MsG0480023713.01.T01
Chr4 exon 88872001 88872174 88872001 ID=MsG0480023713.01.T01:exon:13013;Parent=MsG0480023713.01.T01
Chr4 exon 88872441 88872554 88872441 ID=MsG0480023713.01.T01:exon:13014;Parent=MsG0480023713.01.T01
Chr4 exon 88872647 88872760 88872647 ID=MsG0480023713.01.T01:exon:13015;Parent=MsG0480023713.01.T01
Chr4 CDS 88869938 88870070 88869938 ID=MsG0480023713.01.T01:cds;Parent=MsG0480023713.01.T01
Chr4 CDS 88870683 88871041 88870683 ID=MsG0480023713.01.T01:cds;Parent=MsG0480023713.01.T01
Chr4 CDS 88871312 88871743 88871312 ID=MsG0480023713.01.T01:cds;Parent=MsG0480023713.01.T01
Chr4 CDS 88872001 88872174 88872001 ID=MsG0480023713.01.T01:cds;Parent=MsG0480023713.01.T01
Chr4 CDS 88872441 88872554 88872441 ID=MsG0480023713.01.T01:cds;Parent=MsG0480023713.01.T01
Chr4 CDS 88872647 88872760 88872647 ID=MsG0480023713.01.T01:cds;Parent=MsG0480023713.01.T01
Gene Sequence

>MsG0480023713.01.T01

ATGGCATGCAATGGCATGTTTAGTACATGTCCTTTTGAGGTATCTTTGGTCCAGTATGAAGGGCTTAGAATGACACAAAAAATGCAAATGTTTTCAGCTGCATGCATGCCGAAAAGATACATTTTAGCTTCAGCCAAGAAATGCAAAACAATCAAGGTCAAGGCTTCTCCAACTGTTGCAGCTCCTAAAAGAGAGAAAGATCCAAAAAAGAGAGTAGTAATAACAGGAATGGGTCTTGTGTCAGTCTTTGGAAGTGACATAGATACATTTTACAACAAGCTTCTAGAGGGAGAAAGTGGGATAAGCCTAATAGATAGGTTTGATGCCTCAAGCTTCCCCGTCCGTTTTGGTGGTCAGATACGCGATTTCTCTTCAAAAGGTTACATTGATGGCAAGACTGATCGGCGCCTCGATGATTGCATGAAATATTGTTTAGTTGCAGGAAAGAGGGCACTTGAAGATGCTAATCTTGGATACGAAATCCTTAACAATATGGACAAAACAAGAATAGGAGTTCTAGTAGGATCAGGAATGGGAGGTGTATCCACTTTTAACAATGCTGTTGATGCTCTCACTCGAAAGGGTCATAGGAAAATTTCTCCATTTTTCATTCCCTACACCATTCCTAACAGCAGTTCTGCTTTATTGGCTATAGAAACTGGCCTAATGGGACCAAATTATTCCATTTCTACTGCTTGTGCAACAGCAAATTACTGCTTTTGTGCAGCTTCTCATCACATTAGAAGTGGTGAAGTAGATATTATGGTGGTTGGTGGTACCGAAGCTTCACTTATACCTTCTGGTGTTGGAGCATTCATTGCTTGCAGGGCGTTGTCTCAGAGGAATGAAGAGCCTAAGAAGGCTTCAAGACCATGGGACAAACACCGTGATGGCTTTGTCCTTGGTGAAGGCTCTGGTGTACTGATAATGGAGAGCTTAGAGAGTGCAACAAAGAGGGGAGCAAAAATAATTGCTGAATATTTGGGTGGTGCCATAACATGTGATGCTCATCATATGACTGATCCAAGGTCTGATGGACTTGGTGTTTCATCTTGCATAAACAAGGGTTTAGAAGATGCTGGGGTTTCTCCTGAAGAGGTGAACTATGTGAATGCTCATGCCACATCCACGGTAGCTGGTGACTTGGCTGAGGTTAATGCAATCAAACAAGTTTTTAAGGACACATCAGAATTGAAAATGAACGGGACTAAGTCAATGATTGGTCATTGTATTGGTGCTGCTGGTGCTTTGGAAGCTATAACAACCGTCAAAGCAATAACAACTGGCTGGTTACATCCAACCATTAACCAAGATGTATTGTCTTGA
Protein sequence

>MsG0480023713.01.T01

MACNGMFSTCPFEVSLVQYEGLRMTQKMQMFSAACMPKRYILASAKKCKTIKVKASPTVAAPKREKDPKKRVVITGMGLVSVFGSDIDTFYNKLLEGESGISLIDRFDASSFPVRFGGQIRDFSSKGYIDGKTDRRLDDCMKYCLVAGKRALEDANLGYEILNNMDKTRIGVLVGSGMGGVSTFNNAVDALTRKGHRKISPFFIPYTIPNSSSALLAIETGLMGPNYSISTACATANYCFCAASHHIRSGEVDIMVVGGTEASLIPSGVGAFIACRALSQRNEEPKKASRPWDKHRDGFVLGEGSGVLIMESLESATKRGAKIIAEYLGGAITCDAHHMTDPRSDGLGVSSCINKGLEDAGVSPEEVNYVNAHATSTVAGDLAEVNAIKQVFKDTSELKMNGTKSMIGHCIGAAGALEAITTVKAITTGWLHPTINQDVLS*