Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023862.01.T01 | AET05293.2 | 78.289 | 152 | 25 | 3 | 1 | 152 | 1 | 144 | 6.87E-63 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023862.01.T01 | G7L8Z3 | 78.289 | 152 | 25 | 3 | 1 | 152 | 1 | 144 | 3.28e-63 | 208 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022313.01 | MsG0480023862.01 | 0.802177 | 6.350024e-49 | 3.089845e-46 |
| MsG0480023862.01 | MsG0780040828.01 | 0.802889 | 4.527278e-49 | 2.243366e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023862.01.T01 | MTR_8g103740 | 78.289 | 152 | 25 | 3 | 1 | 152 | 1 | 144 | 8.32e-67 | 208 |
| MsG0480023862.01.T01 | MTR_6g086060 | 46.795 | 156 | 71 | 5 | 1 | 152 | 1 | 148 | 1.11e-34 | 125 |
| MsG0480023862.01.T01 | MTR_8g103740 | 98.000 | 50 | 1 | 0 | 103 | 152 | 1 | 50 | 6.63e-28 | 105 |
| MsG0480023862.01.T01 | MTR_1g046480 | 38.655 | 119 | 66 | 2 | 34 | 152 | 51 | 162 | 8.34e-20 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023862.01.T01 | AT3G24460 | 49.686 | 159 | 64 | 5 | 1 | 152 | 1 | 150 | 6.21e-42 | 144 |
| MsG0480023862.01.T01 | AT3G24460 | 49.686 | 159 | 64 | 5 | 1 | 152 | 1 | 150 | 6.21e-42 | 144 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 1.14e-34 | 122 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 1.14e-34 | 122 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 2.03e-34 | 123 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 5.71e-34 | 123 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 6.62e-34 | 122 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 7.27e-34 | 122 |
| MsG0480023862.01.T01 | AT4G13345 | 52.597 | 154 | 60 | 6 | 1 | 152 | 1 | 143 | 7.27e-34 | 122 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 1.10e-17 | 78.6 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 1.10e-17 | 78.6 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 1.34e-17 | 78.6 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 2.28e-17 | 78.2 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 2.36e-17 | 78.2 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 2.36e-17 | 78.2 |
| MsG0480023862.01.T01 | AT2G33205 | 37.857 | 140 | 74 | 4 | 15 | 152 | 32 | 160 | 2.72e-17 | 77.8 |
Find 33 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCGGCTTTATTCAAATTTA+TGG | 0.115224 | 4:+90616958 | MsG0480023862.01.T01:CDS |
| CACACAATTTGTTGGTTAAA+TGG | 0.126540 | 4:-90616433 | None:intergenic |
| TTGCAGTCCCACGCTTATTA+TGG | 0.248603 | 4:+90616401 | MsG0480023862.01.T01:CDS |
| TCCAAGGATTGGATGCATTT+CGG | 0.359677 | 4:-90616234 | None:intergenic |
| TCATATCCAATGATTCTTCA+TGG | 0.371838 | 4:+90616200 | MsG0480023862.01.T01:CDS |
| GTTGAAGAAGATATGGAAAC+TGG | 0.390734 | 4:+90616148 | None:intergenic |
| TGCTGGCTAATCTGTTGGCA+TGG | 0.404877 | 4:+90616287 | MsG0480023862.01.T01:CDS |
| GCATGGGCTGCTCGTGACTA+TGG | 0.408101 | 4:+90616304 | MsG0480023862.01.T01:CDS |
| TCGTAGGGCTTTAACAGAAA+TGG | 0.428383 | 4:+90616327 | MsG0480023862.01.T01:CDS |
| TCCGAAATGCATCCAATCCT+TGG | 0.436210 | 4:+90616233 | MsG0480023862.01.T01:CDS |
| ACTGAACCATGAAGAATCAT+TGG | 0.442403 | 4:-90616206 | None:intergenic |
| CTGCTCGTGACTATGGTCGT+AGG | 0.472552 | 4:+90616311 | MsG0480023862.01.T01:CDS |
| ACCATAAATTTGAATAAAGC+CGG | 0.484335 | 4:-90616959 | None:intergenic |
| GCTGGCTAATCTGTTGGCAT+GGG | 0.507922 | 4:+90616288 | MsG0480023862.01.T01:CDS |
| AAGTAGAACACACAATTTGT+TGG | 0.522837 | 4:-90616441 | None:intergenic |
| TGCTCGTGACTATGGTCGTA+GGG | 0.527471 | 4:+90616312 | MsG0480023862.01.T01:CDS |
| GGTTAGTCAAGATTGCTTTG+TGG | 0.532718 | 4:+90616902 | MsG0480023862.01.T01:CDS |
| AAGAGAAACACATGGCACTC+TGG | 0.544859 | 4:+90616874 | MsG0480023862.01.T01:CDS |
| ATATCTTGCCATCCAAGGAT+TGG | 0.547635 | 4:-90616245 | None:intergenic |
| TAAGCATATCTTGCCATCCA+AGG | 0.572921 | 4:-90616250 | None:intergenic |
| AAATGCATCCAATCCTTGGA+TGG | 0.580170 | 4:+90616237 | MsG0480023862.01.T01:CDS |
| GAAACACATGGCACTCTGGA+TGG | 0.590908 | 4:+90616878 | MsG0480023862.01.T01:CDS |
| ATGATTTGCCATAATAAGCG+TGG | 0.599949 | 4:-90616409 | None:intergenic |
| ATAAGCGTGGGACTGCAAGA+AGG | 0.603841 | 4:-90616396 | None:intergenic |
| TTGAAGAAGATATGGAAACT+GGG | 0.607550 | 4:+90616149 | None:intergenic |
| GAAACTGGGGAAAGCAACAA+TGG | 0.619670 | 4:+90616163 | MsG0480023862.01.T01:CDS |
| TAAATTTGAATAAAGCCGGA+AGG | 0.631409 | 4:-90616955 | None:intergenic |
| TGATTTGCCATAATAAGCGT+GGG | 0.632235 | 4:-90616408 | None:intergenic |
| GGCTAAATGATATATGACAA+TGG | 0.643792 | 4:-90616375 | None:intergenic |
| TGAAGAAGATATGGAAACTG+GGG | 0.648363 | 4:+90616150 | None:intergenic |
| ACACATGGCACTCTGGATGG+TGG | 0.661486 | 4:+90616881 | MsG0480023862.01.T01:CDS |
| AAAGCAACAATGGTAATGAG+AGG | 0.674245 | 4:+90616173 | MsG0480023862.01.T01:CDS |
| TAAAGCAAAAGAGAAACACA+TGG | 0.747169 | 4:+90616866 | MsG0480023862.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGTTATCATATTCATTTA+TGG | + | Chr4:90616717-90616736 | MsG0480023862.01.T01:intron | 15.0% |
| !! | TAATAATAAATTCAACCTAC+AGG | + | Chr4:90616539-90616558 | MsG0480023862.01.T01:intron | 20.0% |
| !! | TTTCATGTATATAACTGAAA+AGG | - | Chr4:90616641-90616660 | None:intergenic | 20.0% |
| !!! | TTAGATTTGATTTAAACCAA+TGG | - | Chr4:90616522-90616541 | None:intergenic | 20.0% |
| ! | ACCATAAATTTGAATAAAGC+CGG | - | Chr4:90616962-90616981 | None:intergenic | 25.0% |
| ! | CAATGAAAGCAACAATATAT+TGG | - | Chr4:90616471-90616490 | None:intergenic | 25.0% |
| ! | TACATGAAATTAGTCACTAA+AGG | + | Chr4:90616652-90616671 | MsG0480023862.01.T01:intron | 25.0% |
| ! | TCATATTCATTTATGGTGAA+TGG | + | Chr4:90616724-90616743 | MsG0480023862.01.T01:intron | 25.0% |
| !!! | ATAATGTTTTTGTCAACTAC+TGG | + | Chr4:90616832-90616851 | MsG0480023862.01.T01:CDS | 25.0% |
| !!! | ATATATTGTTGCTTTCATTG+AGG | + | Chr4:90616471-90616490 | MsG0480023862.01.T01:intron | 25.0% |
| AAGTAGAACACACAATTTGT+TGG | - | Chr4:90616444-90616463 | None:intergenic | 30.0% | |
| AATTCAACCTACAGGATAAA+AGG | + | Chr4:90616547-90616566 | MsG0480023862.01.T01:intron | 30.0% | |
| GAAAGCAACAATATATTGGA+TGG | - | Chr4:90616467-90616486 | None:intergenic | 30.0% | |
| GGCTAAATGATATATGACAA+TGG | - | Chr4:90616378-90616397 | None:intergenic | 30.0% | |
| TAAAGCAAAAGAGAAACACA+TGG | + | Chr4:90616866-90616885 | MsG0480023862.01.T01:CDS | 30.0% | |
| TAAATTTGAATAAAGCCGGA+AGG | - | Chr4:90616958-90616977 | None:intergenic | 30.0% | |
| TCATATCCAATGATTCTTCA+TGG | + | Chr4:90616200-90616219 | MsG0480023862.01.T01:CDS | 30.0% | |
| TCATATGAATATGATGCCAT+TGG | + | Chr4:90616503-90616522 | MsG0480023862.01.T01:intron | 30.0% | |
| TGATATAGAATGTCTGAACT+TGG | - | Chr4:90616813-90616832 | None:intergenic | 30.0% | |
| ! | TCACTAAAGGCTTTGTATAA+AGG | + | Chr4:90616665-90616684 | MsG0480023862.01.T01:intron | 30.0% |
| !! | CACACAATTTGTTGGTTAAA+TGG | - | Chr4:90616436-90616455 | None:intergenic | 30.0% |
| !! | TCCGGCTTTATTCAAATTTA+TGG | + | Chr4:90616958-90616977 | MsG0480023862.01.T01:CDS | 30.0% |
| ACTGAACCATGAAGAATCAT+TGG | - | Chr4:90616209-90616228 | None:intergenic | 35.0% | |
| ATTAGCCAGCAGAAAAATCA+AGG | - | Chr4:90616278-90616297 | None:intergenic | 35.0% | |
| GAATGTCTGAACTTGGATTA+AGG | - | Chr4:90616806-90616825 | None:intergenic | 35.0% | |
| TACAGGATAAAAGGATGCAA+TGG | + | Chr4:90616556-90616575 | MsG0480023862.01.T01:intron | 35.0% | |
| ! | AAAGCAACAATGGTAATGAG+AGG | + | Chr4:90616173-90616192 | MsG0480023862.01.T01:CDS | 35.0% |
| ! | AATGGTGGAAAAGACTGTTT+GGG | + | Chr4:90616574-90616593 | MsG0480023862.01.T01:intron | 35.0% |
| ! | ATGATTTGCCATAATAAGCG+TGG | - | Chr4:90616412-90616431 | None:intergenic | 35.0% |
| ! | ATTGCATCCTTTTATCCTGT+AGG | - | Chr4:90616557-90616576 | None:intergenic | 35.0% |
| ! | TGATTTGCCATAATAAGCGT+GGG | - | Chr4:90616411-90616430 | None:intergenic | 35.0% |
| ! | TTATGCCTTGATTTTTCTGC+TGG | + | Chr4:90616270-90616289 | MsG0480023862.01.T01:CDS | 35.0% |
| !!! | ATCCCATTTTTTCTACCTTC+CGG | + | Chr4:90616940-90616959 | MsG0480023862.01.T01:CDS | 35.0% |
| AAATGCATCCAATCCTTGGA+TGG | + | Chr4:90616237-90616256 | MsG0480023862.01.T01:CDS | 40.0% | |
| AAGCCGGAAGGTAGAAAAAA+TGG | - | Chr4:90616946-90616965 | None:intergenic | 40.0% | |
| AGCCGGAAGGTAGAAAAAAT+GGG | - | Chr4:90616945-90616964 | None:intergenic | 40.0% | |
| AGGATAAAAGGATGCAATGG+TGG | + | Chr4:90616559-90616578 | MsG0480023862.01.T01:intron | 40.0% | |
| ATATCTTGCCATCCAAGGAT+TGG | - | Chr4:90616248-90616267 | None:intergenic | 40.0% | |
| TAAGCATATCTTGCCATCCA+AGG | - | Chr4:90616253-90616272 | None:intergenic | 40.0% | |
| TCCAAGGATTGGATGCATTT+CGG | - | Chr4:90616237-90616256 | None:intergenic | 40.0% | |
| ! | ATGGTGGAAAAGACTGTTTG+GGG | + | Chr4:90616575-90616594 | MsG0480023862.01.T01:intron | 40.0% |
| ! | CAATGGTGGAAAAGACTGTT+TGG | + | Chr4:90616573-90616592 | MsG0480023862.01.T01:intron | 40.0% |
| ! | GGTTAGTCAAGATTGCTTTG+TGG | + | Chr4:90616902-90616921 | MsG0480023862.01.T01:CDS | 40.0% |
| ! | TCGTAGGGCTTTAACAGAAA+TGG | + | Chr4:90616327-90616346 | MsG0480023862.01.T01:CDS | 40.0% |
| !! | TTTTCTGCTGGCTAATCTGT+TGG | + | Chr4:90616282-90616301 | MsG0480023862.01.T01:CDS | 40.0% |
| AAGAGAAACACATGGCACTC+TGG | + | Chr4:90616874-90616893 | MsG0480023862.01.T01:CDS | 45.0% | |
| GAAACTGGGGAAAGCAACAA+TGG | + | Chr4:90616163-90616182 | MsG0480023862.01.T01:CDS | 45.0% | |
| TCCGAAATGCATCCAATCCT+TGG | + | Chr4:90616233-90616252 | MsG0480023862.01.T01:CDS | 45.0% | |
| TGGTGGAAAAGACTGTTTGG+GGG | + | Chr4:90616576-90616595 | MsG0480023862.01.T01:intron | 45.0% | |
| TTGCAGTCCCACGCTTATTA+TGG | + | Chr4:90616401-90616420 | MsG0480023862.01.T01:CDS | 45.0% | |
| AAAGACTGTTTGGGGGCTGA+AGG | + | Chr4:90616583-90616602 | MsG0480023862.01.T01:intron | 50.0% | |
| ATAAGCGTGGGACTGCAAGA+AGG | - | Chr4:90616399-90616418 | None:intergenic | 50.0% | |
| GAAACACATGGCACTCTGGA+TGG | + | Chr4:90616878-90616897 | MsG0480023862.01.T01:CDS | 50.0% | |
| GCTGGCTAATCTGTTGGCAT+GGG | + | Chr4:90616288-90616307 | MsG0480023862.01.T01:CDS | 50.0% | |
| TGCTCGTGACTATGGTCGTA+GGG | + | Chr4:90616312-90616331 | MsG0480023862.01.T01:CDS | 50.0% | |
| ! | TGCTGGCTAATCTGTTGGCA+TGG | + | Chr4:90616287-90616306 | MsG0480023862.01.T01:CDS | 50.0% |
| ACACATGGCACTCTGGATGG+TGG | + | Chr4:90616881-90616900 | MsG0480023862.01.T01:CDS | 55.0% | |
| CTGCTCGTGACTATGGTCGT+AGG | + | Chr4:90616311-90616330 | MsG0480023862.01.T01:CDS | 55.0% | |
| ! | AGGCGTTTTGCGTGTGAGCT+TGG | + | Chr4:90616603-90616622 | MsG0480023862.01.T01:intron | 55.0% |
| ! | GGCGTTTTGCGTGTGAGCTT+GGG | + | Chr4:90616604-90616623 | MsG0480023862.01.T01:intron | 55.0% |
| ! | GCATGGGCTGCTCGTGACTA+TGG | + | Chr4:90616304-90616323 | MsG0480023862.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 90616160 | 90616987 | 90616160 | ID=MsG0480023862.01;Name=MsG0480023862.01 |
| Chr4 | mRNA | 90616160 | 90616987 | 90616160 | ID=MsG0480023862.01.T01;Parent=MsG0480023862.01;Name=MsG0480023862.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|0|0|2|0|152 |
| Chr4 | exon | 90616160 | 90616450 | 90616160 | ID=MsG0480023862.01.T01:exon:33879;Parent=MsG0480023862.01.T01 |
| Chr4 | exon | 90616820 | 90616987 | 90616820 | ID=MsG0480023862.01.T01:exon:33880;Parent=MsG0480023862.01.T01 |
| Chr4 | CDS | 90616160 | 90616450 | 90616160 | ID=MsG0480023862.01.T01:cds;Parent=MsG0480023862.01.T01 |
| Chr4 | CDS | 90616820 | 90616987 | 90616820 | ID=MsG0480023862.01.T01:cds;Parent=MsG0480023862.01.T01 |
| Gene Sequence |
| Protein sequence |
