AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023995.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023995.01.T01 MTR_8g106740 100.000 41 0 0 34 74 218 258 8.82e-23 89.0
MsG0480023995.01.T02 MTR_8g106740 93.182 176 8 1 1 172 1 176 4.70e-113 322
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023995.01.T02 AT3G18165 63.095 168 59 2 8 172 2 169 2.36e-72 219

Find 22 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGTTGTTGCTTAAATA+TGG 0.210527 4:-92146289 MsG0480023995.01.T01:five_prime_UTR
AATATACAGAATGGAAAATT+TGG 0.215246 4:-92146308 MsG0480023995.01.T01:intron
AATATGAGCCACAGTATAAT+TGG 0.274293 4:+92146201 None:intergenic
TTGGATGCTATCACTGAAAA+TGG 0.288576 4:-92143572 MsG0480023995.01.T01:CDS
TAAATAAACTAAATATTAAT+AGG 0.312752 4:+92143467 None:intergenic
CCTGATGATATTTCCTTTCA+CGG 0.355650 4:+92143912 None:intergenic
TTGTGCTGTTTCCATGCATC+AGG 0.378418 4:+92146265 None:intergenic
TCTTTCCAGAGGCTGGAACT+TGG 0.442445 4:-92143591 MsG0480023995.01.T01:intron
CAGAATGCAGAAATTAGCTC+AGG 0.472152 4:-92143963 MsG0480023995.01.T01:five_prime_UTR
AAAATGGCCAACTGGCAAAT+CGG 0.472926 4:-92143556 MsG0480023995.01.T01:CDS
CATGATTCCGATTTGCCAGT+TGG 0.494066 4:+92143549 None:intergenic
TATCACTGAAAATGGCCAAC+TGG 0.495702 4:-92143564 MsG0480023995.01.T01:CDS
ACAGAGCTCATTCCATTGCA+TGG 0.506599 4:+92143766 None:intergenic
CCGTGAAAGGAAATATCATC+AGG 0.513849 4:-92143912 MsG0480023995.01.T01:intron
TTAAATATGGCCCTGATGCA+TGG 0.568166 4:-92146276 MsG0480023995.01.T01:five_prime_UTR
TGAGAAGAGAAGCAGCAGAA+AGG 0.572177 4:-92143687 MsG0480023995.01.T01:intron
GAAACAGCACAATCAACGAT+TGG 0.581574 4:-92146254 MsG0480023995.01.T01:five_prime_UTR
GATTGGAAGTATACTTGTCA+AGG 0.585048 4:-92146237 MsG0480023995.01.T01:intron
TTGTTCATCCAATTATACTG+TGG 0.596713 4:-92146209 MsG0480023995.01.T01:intron
TTAATGCTTTGTCCATGCAA+TGG 0.598699 4:-92143778 MsG0480023995.01.T01:CDS
TGTGCTGTTTCCATGCATCA+GGG 0.618858 4:+92146266 None:intergenic
CAAAGCATTAAGCTCATATG+CGG 0.683946 4:+92143790 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAACTTAAAAGAAAA+AGG + Chr4:92144780-92144799 None:intergenic 10.0%
!!! TTTTAATTCTTGTTTTTTTT+TGG - Chr4:92145242-92145261 MsG0480023995.01.T01:intron 10.0%
!! GAAAATGAAAATGAAAAATA+TGG + Chr4:92144629-92144648 None:intergenic 15.0%
!!! ATTTTAAAAATAAAGTGACA+AGG + Chr4:92146513-92146532 None:intergenic 15.0%
!!! ATTTTTCTTTTTTCAAAACA+AGG - Chr4:92145576-92145595 MsG0480023995.01.T01:intron 15.0%
!!! CATTCTATTTTTAATGAATA+AGG - Chr4:92145633-92145652 MsG0480023995.01.T01:intron 15.0%
!!! TTTGTAAATTTTGTTTTTCT+TGG - Chr4:92146722-92146741 MsG0480023995.01.T01:five_prime_UTR 15.0%
!!! TTTTAAAAATAAAGTGACAA+GGG + Chr4:92146512-92146531 None:intergenic 15.0%
!! AATATACAGAATGGAAAATT+TGG - Chr4:92144069-92144088 MsG0480023995.01.T01:intron 20.0%
!! AGAACATATTTAATGAATCA+AGG + Chr4:92144569-92144588 None:intergenic 20.0%
!! ATATTTAATGAATCAAGGTT+GGG + Chr4:92144564-92144583 None:intergenic 20.0%
!! ATGAATGTTTCACATAAATA+TGG - Chr4:92145805-92145824 MsG0480023995.01.T01:intron 20.0%
!! ATTGATTACTTTCATGTTTA+GGG - Chr4:92145512-92145531 MsG0480023995.01.T01:intron 20.0%
!! GAAAGAATTATAAAACAGAA+AGG + Chr4:92145101-92145120 None:intergenic 20.0%
!! GTATATTAACTTAAACTAGT+TGG + Chr4:92144056-92144075 None:intergenic 20.0%
!! GTTAATTATTACCTGAAAAT+TGG + Chr4:92143724-92143743 None:intergenic 20.0%
!! TTAAAATCGATGAAAGAAAA+AGG + Chr4:92146316-92146335 None:intergenic 20.0%
!!! AATTCTTCATATTTTTCTCT+TGG - Chr4:92145707-92145726 MsG0480023995.01.T01:intron 20.0%
!!! GTTTAAGTTAATATACAGAA+TGG - Chr4:92144060-92144079 MsG0480023995.01.T01:intron 20.0%
!!! TTTCATCGATTTTAATTTTG+AGG - Chr4:92146321-92146340 MsG0480023995.01.T01:intron 20.0%
! AAGAAAAAGGTGAATAAAAC+TGG + Chr4:92144767-92144786 None:intergenic 25.0%
! AGAACACATTTAATGAATCA+AGG + Chr4:92145348-92145367 None:intergenic 25.0%
! AGATTGATTACTTCATGTTT+AGG - Chr4:92144731-92144750 MsG0480023995.01.T01:intron 25.0%
! ATCAAATGCTATAAACGAAA+GGG - Chr4:92143451-92143470 MsG0480023995.01.T01:three_prime_UTR 25.0%
! ATCTACTACCAATAAGTAAA+TGG + Chr4:92144715-92144734 None:intergenic 25.0%
! ATGTTAGAGACCAAAAAAAT+TGG + Chr4:92146982-92147001 None:intergenic 25.0%
! ATTAAATATGTTCTTGACTC+TGG - Chr4:92144575-92144594 MsG0480023995.01.T01:intron 25.0%
! CATATTTAATGAATCAAGGT+TGG + Chr4:92144565-92144584 None:intergenic 25.0%
! CTTGTTAATTAGGAACTTAA+GGG + Chr4:92145871-92145890 None:intergenic 25.0%
! GAAAAGGAAACTGAAAAATA+TGG + Chr4:92145408-92145427 None:intergenic 25.0%
! GAAGAAAAAGTGAATAAAAC+TGG + Chr4:92145547-92145566 None:intergenic 25.0%
! GATTGATTACTTCATGTTTA+GGG - Chr4:92144732-92144751 MsG0480023995.01.T01:intron 25.0%
! GATTGATTACTTTCATGTTT+AGG - Chr4:92145511-92145530 MsG0480023995.01.T01:intron 25.0%
! TAAAAAAATAGAATGACCCA+TGG + Chr4:92144849-92144868 None:intergenic 25.0%
! TAATATACCTCTTGTTGAAT+TGG - Chr4:92144340-92144359 MsG0480023995.01.T01:intron 25.0%
! TACTTTGTGTTTGTTTGAAA+AGG + Chr4:92145424-92145443 None:intergenic 25.0%
!! AATTTTCCCTTTTCAAAACA+AGG - Chr4:92144797-92144816 MsG0480023995.01.T01:intron 25.0%
!! ACTTGTTAATTAGGAACTTA+AGG + Chr4:92145872-92145891 None:intergenic 25.0%
!! GGTTTGAATACTTGTTAATT+AGG + Chr4:92145881-92145900 None:intergenic 25.0%
!! TTTATTCTTCCATATTGGTT+TGG - Chr4:92145905-92145924 MsG0480023995.01.T01:intron 25.0%
!!! AATTTTTTTGGTCTCTAACA+TGG - Chr4:92146981-92147000 MsG0480023995.01.T01:five_prime_UTR 25.0%
!!! GATAAACATGCCAATTTTTT+TGG - Chr4:92146969-92146988 MsG0480023995.01.T01:five_prime_UTR 25.0%
!!! TCTCAAAATTACTAGTCTTA+AGG - Chr4:92146268-92146287 MsG0480023995.01.T01:five_prime_UTR 25.0%
!!! TTTTAAGACCTTATTAGCTA+CGG - Chr4:92145266-92145285 MsG0480023995.01.T01:intron 25.0%
AAATCAAAACAAAACCCTAG+CGG + Chr4:92143771-92143790 None:intergenic 30.0%
AAATTAAAGTGAAGACCTGA+TGG + Chr4:92143978-92143997 None:intergenic 30.0%
AATATGAGCCACAGTATAAT+TGG + Chr4:92144179-92144198 None:intergenic 30.0%
ACAAAGTATGTTCCTTTAAC+TGG - Chr4:92145436-92145455 MsG0480023995.01.T01:intron 30.0%
ACTTCTATGTCTTCTATCAT+GGG - Chr4:92145609-92145628 MsG0480023995.01.T01:intron 30.0%
ATTAAATGTGTTCTTGACTC+TGG - Chr4:92145354-92145373 MsG0480023995.01.T01:intron 30.0%
ATTACCTGAAAATTGGAAGA+GGG + Chr4:92143717-92143736 None:intergenic 30.0%
CAATATGGAAGAATAAAGTC+AGG + Chr4:92145902-92145921 None:intergenic 30.0%
CATCAAATGCTATAAACGAA+AGG - Chr4:92143450-92143469 MsG0480023995.01.T01:three_prime_UTR 30.0%
CTGACTTTATTCTTCCATAT+TGG - Chr4:92145900-92145919 MsG0480023995.01.T01:intron 30.0%
GATGATGTTTCATGGAAATA+TGG - Chr4:92144388-92144407 MsG0480023995.01.T01:intron 30.0%
GGAAAAGAAGAAATCTCTTA+AGG - Chr4:92144518-92144537 MsG0480023995.01.T01:intron 30.0%
GGAAACTGAAAAATATGGAT+CGG + Chr4:92145403-92145422 None:intergenic 30.0%
GTTGCTTAATGTGAAGAAAA+AGG + Chr4:92146192-92146211 None:intergenic 30.0%
TAAAAATAAAGTGACAAGGG+TGG + Chr4:92146509-92146528 None:intergenic 30.0%
TAACTGGCAATGATGATAAA+AGG - Chr4:92144674-92144693 MsG0480023995.01.T01:intron 30.0%
TACTTCTATGTCTTCTATCA+TGG - Chr4:92145608-92145627 MsG0480023995.01.T01:intron 30.0%
TATTACCTGAAAATTGGAAG+AGG + Chr4:92143718-92143737 None:intergenic 30.0%
TCACATAAATATGGTAGTTG+AGG - Chr4:92145814-92145833 MsG0480023995.01.T01:intron 30.0%
TCATCGTTCCATTTATTAAC+AGG + Chr4:92143902-92143921 None:intergenic 30.0%
TCTACTAGCATAGAAGTAAA+TGG + Chr4:92145493-92145512 None:intergenic 30.0%
TGATTCAGGAGAAAAATAGA+AGG + Chr4:92144891-92144910 None:intergenic 30.0%
TTATCATCATTGCCAGTTAA+AGG + Chr4:92144673-92144692 None:intergenic 30.0%
TTCTTCAATATCAAACCATC+AGG - Chr4:92143960-92143979 MsG0480023995.01.T01:five_prime_UTR 30.0%
TTGAAAAAGTTAACCGTGAA+AGG - Chr4:92146452-92146471 MsG0480023995.01.T01:five_prime_UTR 30.0%
TTGTTCATCCAATTATACTG+TGG - Chr4:92144168-92144187 MsG0480023995.01.T01:intron 30.0%
! AAAATCTTTGAGGAAATCAG+CGG + Chr4:92143685-92143704 None:intergenic 30.0%
! ACAAGAGGTATATTAATCTG+AGG + Chr4:92144335-92144354 None:intergenic 30.0%
! CTCCTTTTTCTTCACATAAA+AGG - Chr4:92146211-92146230 MsG0480023995.01.T01:intron 30.0%
! TATCTCTTTTCGTATAGCAT+TGG - Chr4:92146369-92146388 MsG0480023995.01.T01:intron 30.0%
! TATTTTTCTCCTGAATCCAA+AGG - Chr4:92145670-92145689 MsG0480023995.01.T01:intron 30.0%
! TTACGTGCATTTTATGTACA+AGG + Chr4:92146038-92146057 None:intergenic 30.0%
! TTCTTCTTTTCCAGCTAATA+AGG + Chr4:92144510-92144529 None:intergenic 30.0%
! TTGGAGTTGTTGCTTAAATA+TGG - Chr4:92144088-92144107 MsG0480023995.01.T01:intron 30.0%
!! AATTTGATTTGCACAAGTAC+AGG + Chr4:92144267-92144286 None:intergenic 30.0%
!! ACTAGTAATTTTGAGAGCAT+TGG + Chr4:92146264-92146283 None:intergenic 30.0%
!! TCTATTTTTCTCCTGAATCA+AGG - Chr4:92144891-92144910 MsG0480023995.01.T01:intron 30.0%
!!! ACTTCAACCTTGTTTTGAAA+AGG + Chr4:92144807-92144826 None:intergenic 30.0%
!!! AGTTTTAGAGTGTTAGAAAG+AGG - Chr4:92146105-92146124 MsG0480023995.01.T01:intron 30.0%
!!! CTAGTAATTTTGAGAGCATT+GGG + Chr4:92146263-92146282 None:intergenic 30.0%
!!! CTTCAACCTTGTTTTGAAAA+GGG + Chr4:92144806-92144825 None:intergenic 30.0%
!!! GTTTTAGAGTGTTAGAAAGA+GGG - Chr4:92146106-92146125 MsG0480023995.01.T01:intron 30.0%
!!! GTTTTTTTCTTTCTTTCCAG+AGG - Chr4:92146775-92146794 MsG0480023995.01.T01:five_prime_UTR 30.0%
AACCACCACCTGTTAATAAA+TGG - Chr4:92143891-92143910 MsG0480023995.01.T01:intron 35.0%
AACGAAAGGGTAAATCATGA+TGG - Chr4:92143464-92143483 MsG0480023995.01.T01:exon 35.0%
AATCAAAACAAAACCCTAGC+GGG + Chr4:92143770-92143789 None:intergenic 35.0%
ACAAAGTCTGTTCCTTTAAC+TGG - Chr4:92144658-92144677 MsG0480023995.01.T01:intron 35.0%
ACATCTTTGTCTTCAACCAT+GGG - Chr4:92144830-92144849 MsG0480023995.01.T01:intron 35.0%
ACGAAAGGGTAAATCATGAT+GGG - Chr4:92143465-92143484 MsG0480023995.01.T01:exon 35.0%
AGTTGCTTAGACTGCAATTA+GGG + Chr4:92146169-92146188 None:intergenic 35.0%
ATTCATATCTGGACCTATCA+AGG + Chr4:92143868-92143887 None:intergenic 35.0%
CCTGATGATATTTCCTTTCA+CGG + Chr4:92146468-92146487 None:intergenic 35.0%
CTAAAACTCACTCTCCTATT+GGG + Chr4:92146093-92146112 None:intergenic 35.0%
CTTCAGGATTATCCAATGAT+TGG - Chr4:92143800-92143819 MsG0480023995.01.T01:CDS 35.0%
GATTCTTTAAGTCTCTTGCT+TGG - Chr4:92145976-92145995 MsG0480023995.01.T01:intron 35.0%
GCAGATAAGATGATGTTTCA+TGG - Chr4:92144380-92144399 MsG0480023995.01.T01:intron 35.0%
GGATTCAGGAGAAAAATAGA+AGG + Chr4:92145668-92145687 None:intergenic 35.0%
TAAGTAAGCAAATGATGCAC+AGG + Chr4:92145004-92145023 None:intergenic 35.0%
TACATCTTTGTCTTCAACCA+TGG - Chr4:92144829-92144848 MsG0480023995.01.T01:intron 35.0%
TAGGAATGTTTCACAGCAAA+TGG - Chr4:92145151-92145170 MsG0480023995.01.T01:intron 35.0%
TCAAGAACCAATTCAACAAG+AGG + Chr4:92144350-92144369 None:intergenic 35.0%
TCATACTCTCTACCAATCAT+TGG + Chr4:92143815-92143834 None:intergenic 35.0%
TCGTTCCATTTATTAACAGG+TGG + Chr4:92143899-92143918 None:intergenic 35.0%
TCTTTCTTCCCAAAAGCAAT+TGG - Chr4:92145115-92145134 MsG0480023995.01.T01:intron 35.0%
TTAATGCTTTGTCCATGCAA+TGG - Chr4:92146599-92146618 MsG0480023995.01.T01:intron 35.0%
TTCATATCTGGACCTATCAA+GGG + Chr4:92143867-92143886 None:intergenic 35.0%
TTCCATTTATTAACAGGTGG+TGG + Chr4:92143896-92143915 None:intergenic 35.0%
TTCTTCTTCCGTAGCTAATA+AGG + Chr4:92145277-92145296 None:intergenic 35.0%
TTGATTTACGATTCTGCTTC+AGG - Chr4:92143784-92143803 MsG0480023995.01.T01:CDS 35.0%
TTGGATGCTATCACTGAAAA+TGG - Chr4:92146805-92146824 MsG0480023995.01.T01:five_prime_UTR 35.0%
TTGTTGTATGCAATATCCTG+CGG - Chr4:92145780-92145799 MsG0480023995.01.T01:intron 35.0%
TTTCAACCATGATTGACCTT+TGG + Chr4:92145689-92145708 None:intergenic 35.0%
! AAATCTTTGAGGAAATCAGC+GGG + Chr4:92143684-92143703 None:intergenic 35.0%
! CAAAGCATTAAGCTCATATG+CGG + Chr4:92146590-92146609 None:intergenic 35.0%
! CGCCTTTTATGTGAAGAAAA+AGG + Chr4:92146216-92146235 None:intergenic 35.0%
! GATTGGAAGTATACTTGTCA+AGG - Chr4:92144140-92144159 MsG0480023995.01.T01:intron 35.0%
! TCTAAAACTCACTCTCCTAT+TGG + Chr4:92146094-92146113 None:intergenic 35.0%
!! GACTTTAAGACCTTATTAGC+TGG - Chr4:92144497-92144516 MsG0480023995.01.T01:intron 35.0%
!! TGGCTGATTTCATCTAGTTT+TGG - Chr4:92146742-92146761 MsG0480023995.01.T01:five_prime_UTR 35.0%
!! TGGTGGTTCTAATTCATATC+TGG + Chr4:92143879-92143898 None:intergenic 35.0%
AAAATGAGATCAGTGCACTG+TGG - Chr4:92145745-92145764 MsG0480023995.01.T01:intron 40.0%
AAAATGAGATCAGTGCTCTG+CGG - Chr4:92144964-92144983 MsG0480023995.01.T01:intron 40.0%
AAAATGGCCAACTGGCAAAT+CGG - Chr4:92146821-92146840 MsG0480023995.01.T01:five_prime_UTR 40.0%
AATCCACACAGCAACAAATG+TGG + Chr4:92144221-92144240 None:intergenic 40.0%
AATGGAACGATGAAACTGCT+TGG - Chr4:92143909-92143928 MsG0480023995.01.T01:intron 40.0%
ACACATGAGTTTCACCCAAT+AGG - Chr4:92146076-92146095 MsG0480023995.01.T01:intron 40.0%
ACGGAAGAAGAAATCTCTTG+AGG - Chr4:92145285-92145304 MsG0480023995.01.T01:intron 40.0%
ATATGTCTCGAGCACATCAA+AGG + Chr4:92145847-92145866 None:intergenic 40.0%
ATGATAAAAGGCCGGTCTTA+CGG - Chr4:92144686-92144705 MsG0480023995.01.T01:intron 40.0%
CAGAATGCAGAAATTAGCTC+AGG - Chr4:92146414-92146433 MsG0480023995.01.T01:intron 40.0%
CAGTCATACAGAACAAATGC+AGG + Chr4:92145055-92145074 None:intergenic 40.0%
CATGATTGACCTTTGGATTC+AGG + Chr4:92145682-92145701 None:intergenic 40.0%
CCGTGAAAGGAAATATCATC+AGG - Chr4:92146465-92146484 MsG0480023995.01.T01:five_prime_UTR 40.0%
CTCAAGCAACCAAACCAATA+TGG + Chr4:92145917-92145936 None:intergenic 40.0%
CTGAATCCAAAGGTCAATCA+TGG - Chr4:92145680-92145699 MsG0480023995.01.T01:intron 40.0%
GAAACAGCACAATCAACGAT+TGG - Chr4:92144123-92144142 MsG0480023995.01.T01:intron 40.0%
GAAGAGGTGGAAAATCTTTG+AGG + Chr4:92143695-92143714 None:intergenic 40.0%
GACATTGCATACAACAACCA+AGG + Chr4:92144431-92144450 None:intergenic 40.0%
GAGTTGCTTAGACTGCAATT+AGG + Chr4:92146170-92146189 None:intergenic 40.0%
GGAGAAAGAGTTAGTTAGTG+TGG + Chr4:92143746-92143765 None:intergenic 40.0%
GTATTCCTGGCATTTCTCTA+GGG - Chr4:92146128-92146147 MsG0480023995.01.T01:intron 40.0%
GTTGCATACAACAACAACCA+AGG + Chr4:92145193-92145212 None:intergenic 40.0%
TATCACTGAAAATGGCCAAC+TGG - Chr4:92146813-92146832 MsG0480023995.01.T01:five_prime_UTR 40.0%
TATTCCTGGCATTTCTCTAG+GGG - Chr4:92146129-92146148 MsG0480023995.01.T01:intron 40.0%
TCATATCTGGACCTATCAAG+GGG + Chr4:92143866-92143885 None:intergenic 40.0%
TGAAAATTGGAAGAGGGAAG+AGG + Chr4:92143711-92143730 None:intergenic 40.0%
TGGCAATGATGATAAAAGGC+CGG - Chr4:92144678-92144697 MsG0480023995.01.T01:intron 40.0%
TGGGTGAAACTCATGTGTTA+AGG + Chr4:92146074-92146093 None:intergenic 40.0%
TGTGAAACATTCATATCCGC+AGG + Chr4:92145799-92145818 None:intergenic 40.0%
TTAAATATGGCCCTGATGCA+TGG - Chr4:92144101-92144120 MsG0480023995.01.T01:intron 40.0%
! AAAGCAATTGGCTTAAGCCT+CGG - Chr4:92145127-92145146 MsG0480023995.01.T01:intron 40.0%
! AGGCAATGCATCGATGATTT+CGG + Chr4:92143574-92143593 None:intergenic 40.0%
! AGGCTTAAGCCAATTGCTTT+TGG + Chr4:92145127-92145146 None:intergenic 40.0%
! ATTCCACATTTGTTGCTGTG+TGG - Chr4:92144215-92144234 MsG0480023995.01.T01:intron 40.0%
! TACGGCTGCCATTTACTTAT+TGG - Chr4:92144704-92144723 MsG0480023995.01.T01:intron 40.0%
! TCTTCCCTCTTCCAATTTTC+AGG - Chr4:92143710-92143729 MsG0480023995.01.T01:CDS 40.0%
! TTGTTGTTGTATGCAACTGC+TGG - Chr4:92145196-92145215 MsG0480023995.01.T01:intron 40.0%
! TTTTCTTTCTTTCCAGAGGC+TGG - Chr4:92146779-92146798 MsG0480023995.01.T01:five_prime_UTR 40.0%
!! GGCTTAAGCCAATTGCTTTT+GGG + Chr4:92145126-92145145 None:intergenic 40.0%
!! TGTTAGAAAGAGGGTATTCC+TGG - Chr4:92146115-92146134 MsG0480023995.01.T01:intron 40.0%
!!! ATCTTTTTCGATGCGCAGAT+GGG + Chr4:92146952-92146971 None:intergenic 40.0%
!!! TATCTTTTTCGATGCGCAGA+TGG + Chr4:92146953-92146972 None:intergenic 40.0%
AAATTGGAAGAGGGAAGAGG+TGG + Chr4:92143708-92143727 None:intergenic 45.0%
ACAGAGCTCATTCCATTGCA+TGG + Chr4:92146614-92146633 None:intergenic 45.0%
ACCATGACTGACCTTGATTC+AGG + Chr4:92144905-92144924 None:intergenic 45.0%
ATAAAGTGACAAGGGTGGAC+AGG + Chr4:92146504-92146523 None:intergenic 45.0%
ATGAATCAAGGTTGGGACGA+TGG + Chr4:92144557-92144576 None:intergenic 45.0%
CGACTTCGATCTTAACCCTT+GGG + Chr4:92143620-92143639 None:intergenic 45.0%
CTGTAATCGTCGTCGATGTA+AGG + Chr4:92143594-92143613 None:intergenic 45.0%
CTGTGAAACATTCCTATCCG+AGG + Chr4:92145147-92145166 None:intergenic 45.0%
CTTGAAGCTCCACCTTCTTA+CGG - Chr4:92143528-92143547 MsG0480023995.01.T01:CDS 45.0%
GCATCTCTTCTTCAACGAGA+CGG + Chr4:92143644-92143663 None:intergenic 45.0%
GGCTATGCCTAATGATGCAA+AGG + Chr4:92146242-92146261 None:intergenic 45.0%
GGTATTCCTGGCATTTCTCT+AGG - Chr4:92146127-92146146 MsG0480023995.01.T01:intron 45.0%
TACTTTGAGCATGACAGCCT+TGG - Chr4:92144411-92144430 MsG0480023995.01.T01:intron 45.0%
TCCTGAATCAAGGTCAGTCA+TGG - Chr4:92144901-92144920 MsG0480023995.01.T01:intron 45.0%
TCGACTTCGATCTTAACCCT+TGG + Chr4:92143621-92143640 None:intergenic 45.0%
TGAGAAGAGAAGCAGCAGAA+AGG - Chr4:92146690-92146709 MsG0480023995.01.T01:five_prime_UTR 45.0%
TGTTGTATGCAACTGCTGGT+TGG - Chr4:92145200-92145219 MsG0480023995.01.T01:intron 45.0%
TGTTGTATGCAATGTCCTGC+TGG - Chr4:92144435-92144454 MsG0480023995.01.T01:intron 45.0%
TTATCATTCCACCAACCAGC+AGG + Chr4:92144453-92144472 None:intergenic 45.0%
! AATTGGCTTAAGCCTCGGAT+AGG - Chr4:92145132-92145151 MsG0480023995.01.T01:intron 45.0%
! ATCGGTGCCTCTAGTAAACT+TGG + Chr4:92145385-92145404 None:intergenic 45.0%
! CATGATTCCGATTTGCCAGT+TGG + Chr4:92146831-92146850 None:intergenic 45.0%
! GGGTAAATCATGATGGGAGA+AGG - Chr4:92143471-92143490 MsG0480023995.01.T01:exon 45.0%
! TCGGTGCCTCTAGTAAACTT+GGG + Chr4:92145384-92145403 None:intergenic 45.0%
!! TGTGCTGTTTCCATGCATCA+GGG + Chr4:92144114-92144133 None:intergenic 45.0%
!! TTGGTTTGGTTGCTTGAGTG+AGG - Chr4:92145919-92145938 MsG0480023995.01.T01:intron 45.0%
!! TTGTGCTGTTTCCATGCATC+AGG + Chr4:92144115-92144134 None:intergenic 45.0%
!!! GAGGAAATCAGCGGGTTTTT+TGG + Chr4:92143676-92143695 None:intergenic 45.0%
!! TAAATAAACTAAATATTAAT+AGG + Chr4:92146913-92146932 None:intergenic 5.0%
AAGCTCCACCTTCTTACGGT+AGG - Chr4:92143532-92143551 MsG0480023995.01.T01:CDS 50.0%
AATCAAGGTTGGGACGATGG+AGG + Chr4:92144554-92144573 None:intergenic 50.0%
ACCAGTGCCTCTAGTGAACT+TGG + Chr4:92144606-92144625 None:intergenic 50.0%
CAAAACAAAACCCTAGCGGG+CGG + Chr4:92143767-92143786 None:intergenic 50.0%
CAGTGCCTCTAGTGAACTTG+GGG + Chr4:92144604-92144623 None:intergenic 50.0%
CCAGTGCCTCTAGTGAACTT+GGG + Chr4:92144605-92144624 None:intergenic 50.0%
CGGTGCCTCTAGTAAACTTG+GGG + Chr4:92145383-92145402 None:intergenic 50.0%
CTAGACCTACCGTAAGAAGG+TGG + Chr4:92143540-92143559 None:intergenic 50.0%
GAGTATGAGCGTGTAAGAGC+CGG - Chr4:92143827-92143846 MsG0480023995.01.T01:intron 50.0%
GGAAGAAGCAGCAGCAGTAA+CGG - Chr4:92143492-92143511 MsG0480023995.01.T01:CDS 50.0%
GGCGACTCCTTTGCATCATT+AGG - Chr4:92146232-92146251 MsG0480023995.01.T01:intron 50.0%
GTAAATGGCAGCCGTAAGAC+CGG + Chr4:92144700-92144719 None:intergenic 50.0%
GTACTTGAGCTTGACAGCCT+TGG - Chr4:92145173-92145192 MsG0480023995.01.T01:intron 50.0%
GTATGCAATGTCCTGCTGGT+TGG - Chr4:92144439-92144458 MsG0480023995.01.T01:intron 50.0%
TCTTTCCAGAGGCTGGAACT+TGG - Chr4:92146786-92146805 MsG0480023995.01.T01:five_prime_UTR 50.0%
TGCCTCTAGTAAACTTGGGG+AGG + Chr4:92145380-92145399 None:intergenic 50.0%
TGGCTAGACCTACCGTAAGA+AGG + Chr4:92143543-92143562 None:intergenic 50.0%
TTGTCCCCTAGAGAAATGCC+AGG + Chr4:92146136-92146155 None:intergenic 50.0%
! TGCATCGATGATTTCGGCGT+CGG + Chr4:92143568-92143587 None:intergenic 50.0%
! TGTATGCAACTGCTGGTTGG+TGG - Chr4:92145203-92145222 MsG0480023995.01.T01:intron 50.0%
AACTCTTTCTCCGCCCGCTA+GGG - Chr4:92143754-92143773 MsG0480023995.01.T01:CDS 55.0%
ACGACGATTACAGCGACCCA+AGG - Chr4:92143601-92143620 MsG0480023995.01.T01:intron 55.0%
AGCATCCAAGTTCCAGCCTC+TGG + Chr4:92146794-92146813 None:intergenic 55.0%
ATGAGCGTGTAAGAGCCGGT+AGG - Chr4:92143831-92143850 MsG0480023995.01.T01:intron 55.0%
CGACGATTACAGCGACCCAA+GGG - Chr4:92143602-92143621 MsG0480023995.01.T01:intron 55.0%
CTGGACCTATCAAGGGGAAC+AGG + Chr4:92143860-92143879 None:intergenic 55.0%
GACCTATCAAGGGGAACAGG+AGG + Chr4:92143857-92143876 None:intergenic 55.0%
GGCCTCCCCAAGTTTACTAG+AGG - Chr4:92145375-92145394 MsG0480023995.01.T01:intron 55.0%
TAACTCTTTCTCCGCCCGCT+AGG - Chr4:92143753-92143772 MsG0480023995.01.T01:CDS 55.0%
TGCCTCTAGTGAACTTGGGG+AGG + Chr4:92144601-92144620 None:intergenic 55.0%
! CCCAAGTTCACTAGAGGCAC+TGG - Chr4:92144602-92144621 MsG0480023995.01.T01:intron 55.0%
! CGATGATTTCGGCGTCGGAA+TGG + Chr4:92143563-92143582 None:intergenic 55.0%
! TGCAATGTCCTGCTGGTTGG+TGG - Chr4:92144442-92144461 MsG0480023995.01.T01:intron 55.0%
AAGGGGAACAGGAGGCCTAC+CGG + Chr4:92143849-92143868 None:intergenic 60.0%
GGCCTCCCCAAGTTCACTAG+AGG - Chr4:92144596-92144615 MsG0480023995.01.T01:intron 60.0%
GGCCTCCTGTTCCCCTTGAT+AGG - Chr4:92143852-92143871 MsG0480023995.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 92143397 92147002 92143397 ID=MsG0480023995.01;Name=MsG0480023995.01
Chr4 mRNA 92143397 92147002 92143397 ID=MsG0480023995.01.T01;Parent=MsG0480023995.01;Name=MsG0480023995.01.T01;_AED=0.45;_eAED=0.45;_QI=621|1|1|1|0.6|0.83|6|78|101
Chr4 exon 92143397 92143604 92143397 ID=MsG0480023995.01.T01:exon:8091;Parent=MsG0480023995.01.T01
Chr4 exon 92143688 92143819 92143688 ID=MsG0480023995.01.T01:exon:8090;Parent=MsG0480023995.01.T01
Chr4 exon 92143913 92143982 92143913 ID=MsG0480023995.01.T01:exon:8089;Parent=MsG0480023995.01.T01
Chr4 exon 92146238 92146321 92146238 ID=MsG0480023995.01.T01:exon:8088;Parent=MsG0480023995.01.T01
Chr4 five_prime_UTR 92146668 92147002 92146668 ID=MsG0480023995.01.T01:five_prime_utr;Parent=MsG0480023995.01.T01
Chr4 five_prime_UTR 92146418 92146593 92146418 ID=MsG0480023995.01.T01:five_prime_utr;Parent=MsG0480023995.01.T01
Chr4 five_prime_UTR 92146238 92146321 92146238 ID=MsG0480023995.01.T01:five_prime_utr;Parent=MsG0480023995.01.T01
Chr4 five_prime_UTR 92143957 92143982 92143957 ID=MsG0480023995.01.T01:five_prime_utr;Parent=MsG0480023995.01.T01
Chr4 CDS 92143913 92143956 92143913 ID=MsG0480023995.01.T01:cds;Parent=MsG0480023995.01.T01
Chr4 CDS 92143688 92143819 92143688 ID=MsG0480023995.01.T01:cds;Parent=MsG0480023995.01.T01
Chr4 CDS 92143475 92143604 92143475 ID=MsG0480023995.01.T01:cds;Parent=MsG0480023995.01.T01
Chr4 three_prime_UTR 92143397 92143474 92143397 ID=MsG0480023995.01.T01:three_prime_utr;Parent=MsG0480023995.01.T01
Chr4 mRNA 92146198 92147002 92146198 ID=MsG0480023995.01.T02;Parent=MsG0480023995.01;Name=MsG0480023995.01.T02;_AED=0.47;_eAED=0.50;_QI=83|1|0.66|1|1|1|3|0|183
Chr4 exon 92146198 92146321 92146198 ID=MsG0480023995.01.T02:exon:8092;Parent=MsG0480023995.01.T02
Chr4 five_prime_UTR 92146920 92147002 92146920 ID=MsG0480023995.01.T02:five_prime_utr;Parent=MsG0480023995.01.T02
Chr4 CDS 92146668 92146919 92146668 ID=MsG0480023995.01.T02:cds;Parent=MsG0480023995.01.T02
Chr4 CDS 92146418 92146593 92146418 ID=MsG0480023995.01.T02:cds;Parent=MsG0480023995.01.T02
Chr4 CDS 92146198 92146321 92146198 ID=MsG0480023995.01.T02:cds;Parent=MsG0480023995.01.T02
Gene Sequence

>MsG0480023995.01.T01

ATGATAAGATTGAAAAAGTTAACCGTGAAAGGAAATATCATCAGCAAAACACCGCATATGAGCTTAATGCTTTGTCCATGCAATGGAATGAGCTCTGTCAAAAATATAGACATCAAAGTTGTGTGCGCTTCAGTTGAAACTCACTTAAATGAACTGAGAAGAGAAGCAGCAGAAAGAGGCTGGAACTTGGATGCTATCACTGAAAATGGCCAACTGGCAAATCGGAATCATGAGATTTTTATCAGCTTATCTAGTTTTGTATCTGTAATTCTGACTGCAATTGCTATTGTACCTATTAATATTTAG

>MsG0480023995.01.T02

ATGATGGGAGAAGGAAGAAGCAGCAGCAGTAACGGTGATGTTCTGATGCTTGAAGCTCCACCTTCTTACGGTAGGTCTAGCCATTCCGACGCCGAAATCATCGATGCATTGCCTTACATCGACGACGATTACAGCGACCCAAGGGTTAAGATCGAAGTCGACCGTCTCGTTGAAGAAGAGATGCGTCGCAGCTCCAAAAAACCCGCTGATTTCCTCAAAGATTTTCCACCTCTTCCCTCTTCCAATTTTCAGGATTATCCAATGATTGGTAGAGAGTATGAGCGTGTAAGAGCCGGTAGGCCTCCTGTTCCCCTTGATAGGTCCAGATATGAATTAGAACCACCACCTGTTAATAAATGGAACGATGAAACTGCTTGGAAACTTGCTATTCAACGAGCTCAACGACTTCTTCAATATCAAACCATCAGAATGGAAAATTTGGAGTTGTTGCTTAAATATGGCCCTGATGCATGGAAACAGCACAATCAACGATTGGAAGTATACTTGTCAAGGTTCGTTTGTTCATCCAATTATACTGTGGCTCATATTTAG
Protein sequence

>MsG0480023995.01.T01

MIRLKKLTVKGNIISKTPHMSLMLCPCNGMSSVKNIDIKVVCASVETHLNELRREAAERGWNLDAITENGQLANRNHEIFISLSSFVSVILTAIAIVPINI*

>MsG0480023995.01.T02

MMGEGRSSSSNGDVLMLEAPPSYGRSSHSDAEIIDALPYIDDDYSDPRVKIEVDRLVEEEMRRSSKKPADFLKDFPPLPSSNFQDYPMIGREYERVRAGRPPVPLDRSRYELEPPPVNKWNDETAWKLAIQRAQRLLQYQTIRMENLELLLKYGPDAWKQHNQRLEVYLSRFVCSSNYTVAHI*