AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024047.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024047.01.T01 MTR_5g004910 84.259 432 21 6 1 387 1 430 0.0 696
MsG0580024047.01.T01 MTR_5g004910 76.389 432 15 6 1 387 1 390 0.0 631
MsG0580024047.01.T01 MTR_7g029385 35.071 422 206 10 31 387 113 531 7.77e-69 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024047.01.T01 AT3G19440 47.018 436 160 9 16 387 41 469 2.00e-114 342
MsG0580024047.01.T01 AT3G19440 48.659 261 109 5 16 251 41 301 3.08e-73 232
MsG0580024047.01.T01 AT1G78910 36.782 348 170 5 89 387 20 366 1.93e-69 223
MsG0580024047.01.T01 AT1G78910 35.484 372 190 5 65 387 105 475 1.35e-68 224

Find 117 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACCCTGCACTGGCATTCC+AGG 0.237756 5:+237945 None:intergenic
TTGCAGTGCTTGAGATATAT+TGG 0.249374 5:+235930 None:intergenic
CATAGCATAGGTATACAATT+TGG 0.253664 5:-238488 MsG0580024047.01.T01:five_prime_UTR
TGCTCCCCTTATTCAAATTA+AGG 0.275216 5:+236004 None:intergenic
GTGTTAGGTACACCGATAAT+TGG 0.277960 5:-236135 MsG0580024047.01.T01:CDS
AACTTGTTGTTTAACAGAAT+TGG 0.283089 5:+238073 None:intergenic
GACGAAAGAGTTTATGGATA+AGG 0.301927 5:+238195 None:intergenic
CTTCGACTCCTGCCCTGAAA+TGG 0.310329 5:-238250 MsG0580024047.01.T01:CDS
AAGGAAGAGGCACTCCCTTT+TGG 0.322488 5:-236027 MsG0580024047.01.T01:CDS
CGGTATTGGGCATTGGTTCT+TGG 0.326843 5:-237584 MsG0580024047.01.T01:CDS
GCAGTGAGAGATTCGTGTTT+GGG 0.330924 5:+238038 None:intergenic
CATAGGTATACAATTTGGTT+TGG 0.333516 5:-238483 MsG0580024047.01.T01:five_prime_UTR
ACGAAAGAGTTTATGGATAA+GGG 0.356956 5:+238196 None:intergenic
TTTGGCCTTAATTTGAATAA+GGG 0.361194 5:-236009 MsG0580024047.01.T01:CDS
TGTTAGGTACACCGATAATT+GGG 0.365218 5:-236134 MsG0580024047.01.T01:CDS
ATGCCCAATACCGTCTTTGT+AGG 0.365936 5:+237594 None:intergenic
TGCAGTGCTTGAGATATATT+GGG 0.367867 5:+235931 None:intergenic
AATCCTACAAAGACGGTATT+GGG 0.380224 5:-237597 MsG0580024047.01.T01:CDS
ATAGGTATACAATTTGGTTT+GGG 0.382373 5:-238482 MsG0580024047.01.T01:five_prime_UTR
TGGCAGCGGAAGATGGAAAA+TGG 0.387078 5:-238325 MsG0580024047.01.T01:CDS
ACTTGTTGTTTAACAGAATT+GGG 0.391880 5:+238074 None:intergenic
AACATGATAACAAGTTCAAA+AGG 0.395586 5:-238142 MsG0580024047.01.T01:CDS
TAAACTCTTTCGTCTAAAAC+AGG 0.401047 5:-238186 MsG0580024047.01.T01:CDS
ATAATTGGGGACTACAAGTA+TGG 0.402405 5:-236120 MsG0580024047.01.T01:CDS
CTTGGATGTCCTAGACGTTC+AGG 0.404141 5:-237566 MsG0580024047.01.T01:CDS
ATGAGTCTCATTTCAACATA+TGG 0.409919 5:+235649 None:intergenic
ACAGTTTGATTTGTCTAATC+CGG 0.412494 5:-236073 MsG0580024047.01.T01:CDS
AACATCGTATAACCCATTTC+AGG 0.413289 5:+238237 None:intergenic
GAATCCTACAAAGACGGTAT+TGG 0.413483 5:-237598 MsG0580024047.01.T01:CDS
TGTTATCATGTTGTTGTTGT+GGG 0.414635 5:+238153 None:intergenic
TTACTCTCAACCTCCTCGTT+TGG 0.421311 5:-237868 MsG0580024047.01.T01:CDS
AGCAGTGAGAGATTCGTGTT+TGG 0.423434 5:+238037 None:intergenic
TTTGGGCCCACCCATCTTGA+CGG 0.432617 5:-238465 MsG0580024047.01.T01:five_prime_UTR
TACTTGTAGTCCCCAATTAT+CGG 0.434618 5:+236123 None:intergenic
AGACTAGACAGAGATTGTTC+TGG 0.437828 5:-237840 MsG0580024047.01.T01:CDS
CGGAGTCATTAATGCTCCAC+TGG 0.441021 5:-237543 MsG0580024047.01.T01:CDS
ACCTCCTGGAATGCCAGTGC+AGG 0.441859 5:-237949 MsG0580024047.01.T01:CDS
TACGTTACAAACACACTTGA+AGG 0.445757 5:+235705 None:intergenic
GTCATCATCGGCGGTGGCAG+CGG 0.456844 5:-238339 MsG0580024047.01.T01:CDS
TTCGACTCCTGCCCTGAAAT+GGG 0.457533 5:-238249 MsG0580024047.01.T01:CDS
TTGTTATCATGTTGTTGTTG+TGG 0.457895 5:+238152 None:intergenic
TCGGAGCCGCCGTGAGATCA+TGG 0.463090 5:-238379 MsG0580024047.01.T01:CDS
TCGGCGGTGGCAGCGGAAGA+TGG 0.468461 5:-238332 MsG0580024047.01.T01:CDS
ATATTAATGCCACCCTGCAC+TGG 0.469485 5:+237936 None:intergenic
TTGGAGCTTTCCCCTTTAAC+CGG 0.469543 5:-236312 MsG0580024047.01.T01:CDS
CCTGAGTTCAAAGTATCCTT+AGG 0.470817 5:+238113 None:intergenic
TTATTCAAATTAAGGCCAAA+AGG 0.471079 5:+236012 None:intergenic
GGTCGTTCGTTAATGTTGGC+CGG 0.471922 5:-238444 MsG0580024047.01.T01:five_prime_UTR
ATGGCTCATGAGAATTCAAT+TGG 0.472712 5:-235683 MsG0580024047.01.T01:three_prime_UTR
TCAAGTGTGTTTGTAACGTA+TGG 0.475937 5:-235702 MsG0580024047.01.T01:three_prime_UTR
TGTTCTTCAGTTGAGTCCTC+CGG 0.485992 5:+236054 None:intergenic
AGTAGTTGAATCACTTGAGC+TGG 0.493399 5:-235869 MsG0580024047.01.T01:CDS
TGACGGTCGTTCGTTAATGT+TGG 0.494655 5:-238448 MsG0580024047.01.T01:five_prime_UTR
CAGGAGGTTTGTTTAGAACA+AGG 0.495604 5:+237964 None:intergenic
ATCTCACGGCGGCTCCGACT+CGG 0.498702 5:+238384 None:intergenic
TATTCAAATTAAGGCCAAAA+GGG 0.513142 5:+236013 None:intergenic
CTTGTTCTAAACAAACCTCC+TGG 0.513506 5:-237963 MsG0580024047.01.T01:CDS
ACAAAGACGGTATTGGGCAT+TGG 0.515751 5:-237591 MsG0580024047.01.T01:CDS
GTTCAAAAGGGTTACACCTA+AGG 0.516651 5:-238129 MsG0580024047.01.T01:CDS
CTCAACTGAAGAACATCTCA+AGG 0.534627 5:-236046 MsG0580024047.01.T01:CDS
TTGTTCTGGAATTCTTGTCA+TGG 0.537926 5:-237826 MsG0580024047.01.T01:CDS
CGTATAACCCATTTCAGGGC+AGG 0.539555 5:+238242 None:intergenic
TGTTCTGGAATTCTTGTCAT+GGG 0.542629 5:-237825 MsG0580024047.01.T01:CDS
AACATCAAGGGCTTCAAATG+AGG 0.542967 5:-237754 MsG0580024047.01.T01:intron
TTGGCCTTAATTTGAATAAG+GGG 0.543687 5:-236008 MsG0580024047.01.T01:CDS
TGAATCACTTGAGCTGGAAG+CGG 0.546029 5:-235863 MsG0580024047.01.T01:CDS
TTAACGAACGACCGTCAAGA+TGG 0.551152 5:+238454 None:intergenic
TAACGAACGACCGTCAAGAT+GGG 0.551605 5:+238455 None:intergenic
TCAGTTGTCATCATCGGCGG+TGG 0.552502 5:-238345 MsG0580024047.01.T01:CDS
CCCTGCACTGGCATTCCAGG+AGG 0.555262 5:+237948 None:intergenic
CAGTGAGAGATTCGTGTTTG+GGG 0.555740 5:+238039 None:intergenic
ATTGATAGCAGAATCTCAAA+CGG 0.557918 5:+235734 None:intergenic
TCGTCGTCAGTTGTCATCAT+CGG 0.558310 5:-238351 MsG0580024047.01.T01:CDS
TCGAAGTCGAAGAGGTCACT+TGG 0.561916 5:+238267 None:intergenic
ACATCGTATAACCCATTTCA+GGG 0.562278 5:+238238 None:intergenic
CCTAAGGATACTTTGAACTC+AGG 0.564942 5:-238113 MsG0580024047.01.T01:CDS
GTCCACTGTGCTGAAGTGTT+AGG 0.564993 5:-236150 MsG0580024047.01.T01:CDS
TGATGCAGGTTATACATGGT+TGG 0.566674 5:-236331 MsG0580024047.01.T01:intron
TGAAGAACATCTCAAGGAAG+AGG 0.572591 5:-236040 MsG0580024047.01.T01:CDS
ACATGATAACAAGTTCAAAA+GGG 0.572750 5:-238141 MsG0580024047.01.T01:CDS
GACGGCAAGCTCATAAGAAG+TGG 0.573583 5:-236098 MsG0580024047.01.T01:CDS
TTGTTTAGAACAAGGATGTG+AGG 0.582888 5:+237972 None:intergenic
ATGACTCCGCCTGAACGTCT+AGG 0.583378 5:+237557 None:intergenic
AGGGTGGCATTAATATCAAA+CGG 0.584548 5:-237929 MsG0580024047.01.T01:CDS
AGTGAGAGATTCGTGTTTGG+GGG 0.595365 5:+238040 None:intergenic
GAACGACCGTCAAGATGGGT+GGG 0.599118 5:+238459 None:intergenic
TGAAGAGCATAGCATAGCAT+AGG 0.601779 5:-238500 MsG0580024047.01.T01:five_prime_UTR
GTGAGAGATTCGTGTTTGGG+GGG 0.607139 5:+238041 None:intergenic
ACACTTCAGCACAGTGGACA+CGG 0.608556 5:+236154 None:intergenic
ACGGCAAGCTCATAAGAAGT+GGG 0.609384 5:-236097 MsG0580024047.01.T01:CDS
GGCAGGAGTCGAAGTCGAAG+AGG 0.611989 5:+238259 None:intergenic
ATGTTAGTGCTGAATGCCGC+CGG 0.615059 5:+238425 None:intergenic
CCTCCTGGAATGCCAGTGCA+GGG 0.615398 5:-237948 MsG0580024047.01.T01:CDS
GTTTGATTTGTCTAATCCGG+AGG 0.618245 5:-236070 MsG0580024047.01.T01:CDS
CGAACGACCGTCAAGATGGG+TGG 0.621112 5:+238458 None:intergenic
AAAAGAGAATCCTACAAAGA+CGG 0.623377 5:-237604 MsG0580024047.01.T01:CDS
TTTACAGTAGTAGTACTCAC+AGG 0.632848 5:+238290 None:intergenic
TTGGGGACTACAAGTATGGA+CGG 0.637760 5:-236116 MsG0580024047.01.T01:CDS
GTTAGGTACACCGATAATTG+GGG 0.648978 5:-236133 MsG0580024047.01.T01:CDS
TCTAGTCTATGCACCAAACG+AGG 0.653888 5:+237855 None:intergenic
CGTTCGTTAATGTTGGCCGG+CGG 0.660614 5:-238441 MsG0580024047.01.T01:five_prime_UTR
GGAGTCATTAATGCTCCACT+GGG 0.666010 5:-237542 MsG0580024047.01.T01:intron
GTAATGATGCAGGTTATACA+TGG 0.670336 5:-236335 MsG0580024047.01.T01:intron
GGATGTCCTAGACGTTCAGG+CGG 0.673625 5:-237563 MsG0580024047.01.T01:CDS
TTTAACCGGTAGAAAACACC+AGG 0.674752 5:-236298 MsG0580024047.01.T01:intron
GAAGATGGAATGCAAAAGTG+CGG 0.677074 5:+237785 None:intergenic
ACGACGACTCCATGATCTCA+CGG 0.680735 5:+238370 None:intergenic
TCGTCAGTTGTCATCATCGG+CGG 0.681103 5:-238348 MsG0580024047.01.T01:CDS
CGGCAAGCTCATAAGAAGTG+GGG 0.688690 5:-236096 MsG0580024047.01.T01:CDS
ACGAGTATGTATGTCCGAGT+CGG 0.693334 5:-238398 MsG0580024047.01.T01:exon
CCTGGAATGCCAGTGCAGGG+TGG 0.701700 5:-237945 MsG0580024047.01.T01:CDS
AGTCTATGCACCAAACGAGG+AGG 0.704414 5:+237858 None:intergenic
CAAGTGATTCAACTACTGAG+AGG 0.710001 5:+235875 None:intergenic
TACCTAACACTTCAGCACAG+TGG 0.710893 5:+236148 None:intergenic
ACAGTAGTAGTACTCACAGG+TGG 0.735705 5:+238293 None:intergenic
TTGCAATGAAGATGTAATCG+AGG 0.736658 5:+235967 None:intergenic
ACGACTCCATGATCTCACGG+CGG 0.860711 5:+238373 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAACTTATCTTTAGTTT+TGG - Chr5:237279-237298 MsG0580024047.01.T01:intron 15.0%
!! AACAATCAGAATAGAAATTA+TGG + Chr5:237934-237953 None:intergenic 20.0%
!! ACAATCAGAATAGAAATTAT+GGG + Chr5:237933-237952 None:intergenic 20.0%
!! TAATAAAGATTAAGCCTTTA+CGG + Chr5:236911-236930 None:intergenic 20.0%
!!! AGTTTACGTTTAAAATTTAG+AGG - Chr5:237152-237171 MsG0580024047.01.T01:intron 20.0%
!!! CTTCAATTTAATTTCATGTT+TGG - Chr5:237555-237574 MsG0580024047.01.T01:CDS 20.0%
! AAAACTAAACATAACAAAGG+GGG + Chr5:237714-237733 None:intergenic 25.0%
! AACATGATAACAAGTTCAAA+AGG - Chr5:235984-236003 MsG0580024047.01.T01:CDS 25.0%
! AACATTGTGATTTAACTGAA+AGG + Chr5:236643-236662 None:intergenic 25.0%
! ACAACATTATTGACAATATG+AGG + Chr5:236984-237003 None:intergenic 25.0%
! ACATGATAACAAGTTCAAAA+GGG - Chr5:235985-236004 MsG0580024047.01.T01:CDS 25.0%
! AGCAATAAAGATAATTTGAG+AGG + Chr5:237305-237324 None:intergenic 25.0%
! AGGAAAACTAAACATAACAA+AGG + Chr5:237717-237736 None:intergenic 25.0%
! CAATCAGAATAGAAATTATG+GGG + Chr5:237932-237951 None:intergenic 25.0%
! GAAAACTAAACATAACAAAG+GGG + Chr5:237715-237734 None:intergenic 25.0%
! GGAAAACTAAACATAACAAA+GGG + Chr5:237716-237735 None:intergenic 25.0%
! TATTCAAATTAAGGCCAAAA+GGG + Chr5:238116-238135 None:intergenic 25.0%
! TTATTCAAATTAAGGCCAAA+AGG + Chr5:238117-238136 None:intergenic 25.0%
!! ATAGGTATACAATTTGGTTT+GGG - Chr5:235644-235663 MsG0580024047.01.T01:three_prime_UTR 25.0%
!! TAAATCACAATGTTTTGCAA+TGG - Chr5:236649-236668 MsG0580024047.01.T01:intron 25.0%
!! TCACAAATTTTCCATATTTC+AGG + Chr5:237675-237694 None:intergenic 25.0%
!! TTTTGTAAACTCAACTAGTT+TGG + Chr5:237437-237456 None:intergenic 25.0%
!!! AACTTGTTGTTTAACAGAAT+TGG + Chr5:236056-236075 None:intergenic 25.0%
!!! AATGGATCTTTATTTTCTCT+TGG - Chr5:236667-236686 MsG0580024047.01.T01:intron 25.0%
!!! ACTTGTTGTTTAACAGAATT+GGG + Chr5:236055-236074 None:intergenic 25.0%
!!! GGAAAATAGAATTTTAGTCT+GGG - Chr5:237576-237595 MsG0580024047.01.T01:CDS 25.0%
!!! GTGTTTTAAATTTAGTTCAC+TGG + Chr5:236785-236804 None:intergenic 25.0%
!!! TGGAAAATAGAATTTTAGTC+TGG - Chr5:237575-237594 MsG0580024047.01.T01:CDS 25.0%
!!! TTTGGCCTTAATTTGAATAA+GGG - Chr5:238117-238136 MsG0580024047.01.T01:CDS 25.0%
!!! TTTTGGCCTTAATTTGAATA+AGG - Chr5:238116-238135 MsG0580024047.01.T01:CDS 25.0%
AAAAGAGAATCCTACAAAGA+CGG - Chr5:236522-236541 MsG0580024047.01.T01:intron 30.0%
AAACTAAACATAACAAAGGG+GGG + Chr5:237713-237732 None:intergenic 30.0%
AAAGCAGTAAAGGAAACATA+GGG + Chr5:236748-236767 None:intergenic 30.0%
AAATGTTTGAGCCTGAAATA+TGG - Chr5:237661-237680 MsG0580024047.01.T01:intron 30.0%
AAGGAAACATAGGGTAAAAT+AGG + Chr5:236739-236758 None:intergenic 30.0%
ACGAAAGAGTTTATGGATAA+GGG + Chr5:235933-235952 None:intergenic 30.0%
ATGAGTCTCATTTCAACATA+TGG + Chr5:238480-238499 None:intergenic 30.0%
CAAGTAATGTAAAGCAGTAA+AGG + Chr5:236758-236777 None:intergenic 30.0%
CATAGCATAGGTATACAATT+TGG - Chr5:235638-235657 MsG0580024047.01.T01:three_prime_UTR 30.0%
CATAGGTATACAATTTGGTT+TGG - Chr5:235643-235662 MsG0580024047.01.T01:three_prime_UTR 30.0%
CTGAAACCAAATCAAATGAA+GGG + Chr5:237009-237028 None:intergenic 30.0%
TAAAGCAGTAAAGGAAACAT+AGG + Chr5:236749-236768 None:intergenic 30.0%
TGTTGTGATAGAAATCATGT+AGG + Chr5:237630-237649 None:intergenic 30.0%
TTACTGCTTTACATTACTTG+TGG - Chr5:236758-236777 MsG0580024047.01.T01:intron 30.0%
TTCACATCATCACATGTTAT+TGG - Chr5:237486-237505 MsG0580024047.01.T01:intron 30.0%
TTGTAACCCTTCATTTGATT+TGG - Chr5:237000-237019 MsG0580024047.01.T01:intron 30.0%
TTGTATCCATATTCTCAACT+TGG - Chr5:236612-236631 MsG0580024047.01.T01:intron 30.0%
! AGTTTATGGATAAGGGTTTT+AGG + Chr5:235926-235945 None:intergenic 30.0%
! ATTGATAGCAGAATCTCAAA+CGG + Chr5:238395-238414 None:intergenic 30.0%
! TAAACTCTTTCGTCTAAAAC+AGG - Chr5:235940-235959 MsG0580024047.01.T01:CDS 30.0%
! TGAACTTGTGTTTTATAGCA+CGG - Chr5:237207-237226 MsG0580024047.01.T01:intron 30.0%
! TGTTATCATGTTGTTGTTGT+GGG + Chr5:235976-235995 None:intergenic 30.0%
! TTGTTATCATGTTGTTGTTG+TGG + Chr5:235977-235996 None:intergenic 30.0%
!! ACAGTTTGATTTGTCTAATC+CGG - Chr5:238053-238072 MsG0580024047.01.T01:CDS 30.0%
!! AGCAGTCTTTTTAATTCACT+TGG - Chr5:238349-238368 MsG0580024047.01.T01:CDS 30.0%
!! GTTTTAGACGAAAGAGTTTA+TGG + Chr5:235940-235959 None:intergenic 30.0%
!! TTGGCCTTAATTTGAATAAG+GGG - Chr5:238118-238137 MsG0580024047.01.T01:CDS 30.0%
!!! AGCATGCATTTTTTGCATTT+CGG + Chr5:237413-237432 None:intergenic 30.0%
!!! ATTTTAGTTTCCATAGTGTG+TGG - Chr5:236806-236825 MsG0580024047.01.T01:intron 30.0%
AAAGATGAAATGATAGCAGC+AGG + Chr5:236477-236496 None:intergenic 35.0%
AACACCAGGTAATAGAAACA+AGG - Chr5:237842-237861 MsG0580024047.01.T01:CDS 35.0%
AACATCGTATAACCCATTTC+AGG + Chr5:235892-235911 None:intergenic 35.0%
AATCCTACAAAGACGGTATT+GGG - Chr5:236529-236548 MsG0580024047.01.T01:intron 35.0%
AATTAAGATCTCCCTCCTTA+TGG - Chr5:237382-237401 MsG0580024047.01.T01:intron 35.0%
ACACCAGGTAATAGAAACAA+GGG - Chr5:237843-237862 MsG0580024047.01.T01:CDS 35.0%
ACATCGTATAACCCATTTCA+GGG + Chr5:235891-235910 None:intergenic 35.0%
AGGCTCAAACATTTGCAATT+AGG + Chr5:237655-237674 None:intergenic 35.0%
AGGGTGGCATTAATATCAAA+CGG - Chr5:236197-236216 MsG0580024047.01.T01:intron 35.0%
ATAATTGGGGACTACAAGTA+TGG - Chr5:238006-238025 MsG0580024047.01.T01:CDS 35.0%
ATACATGAAAAAGAGTTGGG+AGG - Chr5:238296-238315 MsG0580024047.01.T01:CDS 35.0%
ATGGCTCATGAGAATTCAAT+TGG - Chr5:238443-238462 MsG0580024047.01.T01:five_prime_UTR 35.0%
ATTTCGGTAATTCCATAAGG+AGG + Chr5:237397-237416 None:intergenic 35.0%
CAATATGCAATAGTCCGTAA+AGG - Chr5:236894-236913 MsG0580024047.01.T01:intron 35.0%
CCCATACATGAAAAAGAGTT+GGG - Chr5:238293-238312 MsG0580024047.01.T01:CDS 35.0%
CCTGAAACCAAATCAAATGA+AGG + Chr5:237010-237029 None:intergenic 35.0%
CTTCCGTGAAAAAACATCAA+GGG - Chr5:236360-236379 MsG0580024047.01.T01:intron 35.0%
GACGAAAGAGTTTATGGATA+AGG + Chr5:235934-235953 None:intergenic 35.0%
GCACAAGTAATTAAACCGAT+AGG - Chr5:237598-237617 MsG0580024047.01.T01:CDS 35.0%
GTAATGATGCAGGTTATACA+TGG - Chr5:237791-237810 MsG0580024047.01.T01:CDS 35.0%
TACGTTACAAACACACTTGA+AGG + Chr5:238424-238443 None:intergenic 35.0%
TACTTGTAGTCCCCAATTAT+CGG + Chr5:238006-238025 None:intergenic 35.0%
TATTGCATATTGCAGCTGTA+AGG + Chr5:236886-236905 None:intergenic 35.0%
TCAAGTGTGTTTGTAACGTA+TGG - Chr5:238424-238443 MsG0580024047.01.T01:five_prime_UTR 35.0%
TCTATTCTGATTGTTTGTGC+TGG - Chr5:237939-237958 MsG0580024047.01.T01:CDS 35.0%
TCTCCTTTGATCTTCTTAGT+TGG - Chr5:237747-237766 MsG0580024047.01.T01:intron 35.0%
TCTTCCGTGAAAAAACATCA+AGG - Chr5:236359-236378 MsG0580024047.01.T01:intron 35.0%
TGCAGTGCTTGAGATATATT+GGG + Chr5:238198-238217 None:intergenic 35.0%
TGCATTTCGGTAATTCCATA+AGG + Chr5:237400-237419 None:intergenic 35.0%
TGCTCCCCTTATTCAAATTA+AGG + Chr5:238125-238144 None:intergenic 35.0%
TGTTAGGTACACCGATAATT+GGG - Chr5:237992-238011 MsG0580024047.01.T01:CDS 35.0%
TTATAGCACGGCATAATTCT+GGG - Chr5:237219-237238 MsG0580024047.01.T01:intron 35.0%
TTGCAATGAAGATGTAATCG+AGG + Chr5:238162-238181 None:intergenic 35.0%
TTGCAGTGCTTGAGATATAT+TGG + Chr5:238199-238218 None:intergenic 35.0%
TTGCCAACTAAGAAGATCAA+AGG + Chr5:237753-237772 None:intergenic 35.0%
TTTACAGTAGTAGTACTCAC+AGG + Chr5:235839-235858 None:intergenic 35.0%
TTTATAGCACGGCATAATTC+TGG - Chr5:237218-237237 MsG0580024047.01.T01:intron 35.0%
TTTCGGTAATTCCATAAGGA+GGG + Chr5:237396-237415 None:intergenic 35.0%
! GACAATATGAGGAGCTTTTA+TGG + Chr5:236973-236992 None:intergenic 35.0%
! GATTAGCCAAGTTGAGAATA+TGG + Chr5:236621-236640 None:intergenic 35.0%
! TATTACCTGGTGTTTTCTAC+CGG + Chr5:237836-237855 None:intergenic 35.0%
! TTGTTTAGAACAAGGATGTG+AGG + Chr5:236157-236176 None:intergenic 35.0%
!! CAACTCTTTTTCATGTATGG+GGG + Chr5:238294-238313 None:intergenic 35.0%
!! CCAACTCTTTTTCATGTATG+GGG + Chr5:238295-238314 None:intergenic 35.0%
!! CCCAACTCTTTTTCATGTAT+GGG + Chr5:238296-238315 None:intergenic 35.0%
!! CCTTCATTTGATTTGGTTTC+AGG - Chr5:237007-237026 MsG0580024047.01.T01:intron 35.0%
!! TCCCAACTCTTTTTCATGTA+TGG + Chr5:238297-238316 None:intergenic 35.0%
!! TGTTCTGGAATTCTTGTCAT+GGG - Chr5:236301-236320 MsG0580024047.01.T01:CDS 35.0%
!! TTGTTCTGGAATTCTTGTCA+TGG - Chr5:236300-236319 MsG0580024047.01.T01:CDS 35.0%
!!! AAGCCCTTGATGTTTTTTCA+CGG + Chr5:236366-236385 None:intergenic 35.0%
!!! ATAAGGGTTTTAGGTAGTTG+AGG + Chr5:235917-235936 None:intergenic 35.0%
!!! TAAGGGTTTTAGGTAGTTGA+GGG + Chr5:235916-235935 None:intergenic 35.0%
!!! TGATGTTTTTTCACGGAAGA+TGG + Chr5:236359-236378 None:intergenic 35.0%
AACATCAAGGGCTTCAAATG+AGG - Chr5:236372-236391 MsG0580024047.01.T01:intron 40.0%
AATGTCACAGACACTGCAAA+AGG + Chr5:237260-237279 None:intergenic 40.0%
AGACTAGACAGAGATTGTTC+TGG - Chr5:236286-236305 MsG0580024047.01.T01:intron 40.0%
AGTAGTTGAATCACTTGAGC+TGG - Chr5:238257-238276 MsG0580024047.01.T01:CDS 40.0%
ATGTCACAGACACTGCAAAA+GGG + Chr5:237259-237278 None:intergenic 40.0%
ATGTGATAGCAGAGAGTTGT+TGG + Chr5:236852-236871 None:intergenic 40.0%
CAAGTGATTCAACTACTGAG+AGG + Chr5:238254-238273 None:intergenic 40.0%
CCCCATACATGAAAAAGAGT+TGG - Chr5:238292-238311 MsG0580024047.01.T01:CDS 40.0%
CCTAAGGATACTTTGAACTC+AGG - Chr5:236013-236032 MsG0580024047.01.T01:CDS 40.0%
CTCAACTGAAGAACATCTCA+AGG - Chr5:238080-238099 MsG0580024047.01.T01:CDS 40.0%
CTGCTATCACATGAATGCTT+AGG - Chr5:236860-236879 MsG0580024047.01.T01:intron 40.0%
GAAGATGGAATGCAAAAGTG+CGG + Chr5:236344-236363 None:intergenic 40.0%
GAATCATTACCAGCTGATCT+TGG - Chr5:237868-237887 MsG0580024047.01.T01:CDS 40.0%
GAATCCTACAAAGACGGTAT+TGG - Chr5:236528-236547 MsG0580024047.01.T01:intron 40.0%
GAGACGATGATGAAATCACT+CGG + Chr5:237118-237137 None:intergenic 40.0%
GTGATTTCATCATCGTCTCA+AGG - Chr5:237119-237138 MsG0580024047.01.T01:intron 40.0%
GTGTTAGGTACACCGATAAT+TGG - Chr5:237991-238010 MsG0580024047.01.T01:CDS 40.0%
GTTAGGTACACCGATAATTG+GGG - Chr5:237993-238012 MsG0580024047.01.T01:CDS 40.0%
GTTCAAAAGGGTTACACCTA+AGG - Chr5:235997-236016 MsG0580024047.01.T01:CDS 40.0%
TAAACATAACAAAGGGGGGA+GGG + Chr5:237709-237728 None:intergenic 40.0%
TCATGTAGGACATCACCTAT+CGG + Chr5:237616-237635 None:intergenic 40.0%
TCATTACCAGTGACATGAGT+CGG + Chr5:237778-237797 None:intergenic 40.0%
TGAAGAACATCTCAAGGAAG+AGG - Chr5:238086-238105 MsG0580024047.01.T01:CDS 40.0%
TGATGCAGGTTATACATGGT+TGG - Chr5:237795-237814 MsG0580024047.01.T01:CDS 40.0%
TGGCAATCATTGTGTATACG+AGG + Chr5:236832-236851 None:intergenic 40.0%
TTTAACCGGTAGAAAACACC+AGG - Chr5:237828-237847 MsG0580024047.01.T01:CDS 40.0%
TTTCATCATCGTCTCAAGGT+TGG - Chr5:237123-237142 MsG0580024047.01.T01:intron 40.0%
! CAGGAGGTTTGTTTAGAACA+AGG + Chr5:236165-236184 None:intergenic 40.0%
! CCTGAGTTCAAAGTATCCTT+AGG + Chr5:236016-236035 None:intergenic 40.0%
! CTTGTTCTAAACAAACCTCC+TGG - Chr5:236163-236182 MsG0580024047.01.T01:intron 40.0%
! TCAAAGGAGACACGTTTTGA+AGG + Chr5:237737-237756 None:intergenic 40.0%
! TGAAGAGCATAGCATAGCAT+AGG - Chr5:235626-235645 MsG0580024047.01.T01:three_prime_UTR 40.0%
!! CTTCCCTTGTTTCTATTACC+TGG + Chr5:237849-237868 None:intergenic 40.0%
!! GGTGTTTTCTACCGGTTAAA+GGG + Chr5:237828-237847 None:intergenic 40.0%
!! GTGTTTTCTACCGGTTAAAG+GGG + Chr5:237827-237846 None:intergenic 40.0%
!! GTTTGATTTGTCTAATCCGG+AGG - Chr5:238056-238075 MsG0580024047.01.T01:CDS 40.0%
!! TGGTGTTTTCTACCGGTTAA+AGG + Chr5:237829-237848 None:intergenic 40.0%
!! TTTGATTTGGTTTCAGGTCG+TGG - Chr5:237013-237032 MsG0580024047.01.T01:intron 40.0%
AAACATAACAAAGGGGGGAG+GGG + Chr5:237708-237727 None:intergenic 45.0%
ACAAGTTCACCCACACTTAC+AGG + Chr5:237196-237215 None:intergenic 45.0%
ACAGTAGTAGTACTCACAGG+TGG + Chr5:235836-235855 None:intergenic 45.0%
ACGAGTATGTATGTCCGAGT+CGG - Chr5:235728-235747 MsG0580024047.01.T01:three_prime_UTR 45.0%
ACGGCAAGCTCATAAGAAGT+GGG - Chr5:238029-238048 MsG0580024047.01.T01:CDS 45.0%
AGCAGTGAGAGATTCGTGTT+TGG + Chr5:236092-236111 None:intergenic 45.0%
AGTGAGAGATTCGTGTTTGG+GGG + Chr5:236089-236108 None:intergenic 45.0%
ATATTAATGCCACCCTGCAC+TGG + Chr5:236193-236212 None:intergenic 45.0%
ATGCCCAATACCGTCTTTGT+AGG + Chr5:236535-236554 None:intergenic 45.0%
ATGGATACAACCTTACCCAG+TGG + Chr5:236602-236621 None:intergenic 45.0%
CAAGTTCACCCACACTTACA+GGG + Chr5:237195-237214 None:intergenic 45.0%
CAGTGAGAGATTCGTGTTTG+GGG + Chr5:236090-236109 None:intergenic 45.0%
CATTAATGCTCCACTGGGTA+AGG - Chr5:236589-236608 MsG0580024047.01.T01:intron 45.0%
CATTACCAGTGACATGAGTC+GGG + Chr5:237777-237796 None:intergenic 45.0%
CGAGCTGATAGCTACATAAC+AGG - Chr5:237527-237546 MsG0580024047.01.T01:intron 45.0%
CTAAACATAACAAAGGGGGG+AGG + Chr5:237710-237729 None:intergenic 45.0%
CTGATAGCTACATAACAGGC+AGG - Chr5:237531-237550 MsG0580024047.01.T01:intron 45.0%
GCAGTGAGAGATTCGTGTTT+GGG + Chr5:236091-236110 None:intergenic 45.0%
GGAGTCATTAATGCTCCACT+GGG - Chr5:236584-236603 MsG0580024047.01.T01:intron 45.0%
GTGATTGTCCCTGTAAGTGT+GGG - Chr5:237184-237203 MsG0580024047.01.T01:intron 45.0%
GTTGTAGTACCAAGATCAGC+TGG + Chr5:237880-237899 None:intergenic 45.0%
TAACGAACGACCGTCAAGAT+GGG + Chr5:235674-235693 None:intergenic 45.0%
TACCTAACACTTCAGCACAG+TGG + Chr5:237981-238000 None:intergenic 45.0%
TCGTCGTCAGTTGTCATCAT+CGG - Chr5:235775-235794 MsG0580024047.01.T01:three_prime_UTR 45.0%
TCTAGTCTATGCACCAAACG+AGG + Chr5:236274-236293 None:intergenic 45.0%
TGAATCACTTGAGCTGGAAG+CGG - Chr5:238263-238282 MsG0580024047.01.T01:CDS 45.0%
TGACGGTCGTTCGTTAATGT+TGG - Chr5:235678-235697 MsG0580024047.01.T01:three_prime_UTR 45.0%
TGTGATTGTCCCTGTAAGTG+TGG - Chr5:237183-237202 MsG0580024047.01.T01:intron 45.0%
TGTTCTTCAGTTGAGTCCTC+CGG + Chr5:238075-238094 None:intergenic 45.0%
TTAACGAACGACCGTCAAGA+TGG + Chr5:235675-235694 None:intergenic 45.0%
TTACTCTCAACCTCCTCGTT+TGG - Chr5:236258-236277 MsG0580024047.01.T01:intron 45.0%
TTGGAGCTTTCCCCTTTAAC+CGG - Chr5:237814-237833 MsG0580024047.01.T01:CDS 45.0%
TTGGGGACTACAAGTATGGA+CGG - Chr5:238010-238029 MsG0580024047.01.T01:CDS 45.0%
! ACAAAGACGGTATTGGGCAT+TGG - Chr5:236535-236554 MsG0580024047.01.T01:intron 45.0%
! CATGTCACTGGTAATGATGC+AGG - Chr5:237781-237800 MsG0580024047.01.T01:CDS 45.0%
! TTTCAGGTCGTGGTTGACAA+CGG - Chr5:237023-237042 MsG0580024047.01.T01:intron 45.0%
AACATAACAAAGGGGGGAGG+GGG + Chr5:237707-237726 None:intergenic 50.0%
ACACTTCAGCACAGTGGACA+CGG + Chr5:237975-237994 None:intergenic 50.0%
ACGACGACTCCATGATCTCA+CGG + Chr5:235759-235778 None:intergenic 50.0%
AGTCTATGCACCAAACGAGG+AGG + Chr5:236271-236290 None:intergenic 50.0%
CGGAGTCATTAATGCTCCAC+TGG - Chr5:236583-236602 MsG0580024047.01.T01:intron 50.0%
CGGCAAGCTCATAAGAAGTG+GGG - Chr5:238030-238049 MsG0580024047.01.T01:CDS 50.0%
CGTATAACCCATTTCAGGGC+AGG + Chr5:235887-235906 None:intergenic 50.0%
CTTGGATGTCCTAGACGTTC+AGG - Chr5:236560-236579 MsG0580024047.01.T01:intron 50.0%
GACGGCAAGCTCATAAGAAG+TGG - Chr5:238028-238047 MsG0580024047.01.T01:CDS 50.0%
GGTCGTTCGTTAATGTTGGC+CGG - Chr5:235682-235701 MsG0580024047.01.T01:three_prime_UTR 50.0%
GTATACGAGGCCACACACTA+TGG + Chr5:236819-236838 None:intergenic 50.0%
GTGAGAGATTCGTGTTTGGG+GGG + Chr5:236088-236107 None:intergenic 50.0%
TCGAAGTCGAAGAGGTCACT+TGG + Chr5:235862-235881 None:intergenic 50.0%
TCGTCAGTTGTCATCATCGG+CGG - Chr5:235778-235797 MsG0580024047.01.T01:three_prime_UTR 50.0%
TGGCAGCGGAAGATGGAAAA+TGG - Chr5:235801-235820 MsG0580024047.01.T01:exon 50.0%
TTCGACTCCTGCCCTGAAAT+GGG - Chr5:235877-235896 MsG0580024047.01.T01:CDS 50.0%
! ATGTTAGTGCTGAATGCCGC+CGG + Chr5:235704-235723 None:intergenic 50.0%
! GTCCACTGTGCTGAAGTGTT+AGG - Chr5:237976-237995 MsG0580024047.01.T01:CDS 50.0%
!! CGGTATTGGGCATTGGTTCT+TGG - Chr5:236542-236561 MsG0580024047.01.T01:intron 50.0%
!!! AAGGAAGAGGCACTCCCTTT+TGG - Chr5:238099-238118 MsG0580024047.01.T01:CDS 50.0%
ACATAACAAAGGGGGGAGGG+GGG + Chr5:237706-237725 None:intergenic 55.0%
ACGACTCCATGATCTCACGG+CGG + Chr5:235756-235775 None:intergenic 55.0%
ATGACTCCGCCTGAACGTCT+AGG + Chr5:236572-236591 None:intergenic 55.0%
CGTTCGTTAATGTTGGCCGG+CGG - Chr5:235685-235704 MsG0580024047.01.T01:three_prime_UTR 55.0%
CTTCGACTCCTGCCCTGAAA+TGG - Chr5:235876-235895 MsG0580024047.01.T01:CDS 55.0%
GAACGACCGTCAAGATGGGT+GGG + Chr5:235670-235689 None:intergenic 55.0%
GCAATCCCGACTCATGTCAC+TGG - Chr5:237769-237788 MsG0580024047.01.T01:CDS 55.0%
GGATGTCCTAGACGTTCAGG+CGG - Chr5:236563-236582 MsG0580024047.01.T01:intron 55.0%
TCAGTTGTCATCATCGGCGG+TGG - Chr5:235781-235800 MsG0580024047.01.T01:three_prime_UTR 55.0%
TTTGGGCCCACCCATCTTGA+CGG - Chr5:235661-235680 MsG0580024047.01.T01:three_prime_UTR 55.0%
! GATTCGTGTTTGGGGGGTGT+AGG + Chr5:236082-236101 None:intergenic 55.0%
ACCTCCTGGAATGCCAGTGC+AGG - Chr5:236177-236196 MsG0580024047.01.T01:intron 60.0%
CCTCCTGGAATGCCAGTGCA+GGG - Chr5:236178-236197 MsG0580024047.01.T01:intron 60.0%
CGAACGACCGTCAAGATGGG+TGG + Chr5:235671-235690 None:intergenic 60.0%
GGCAGGAGTCGAAGTCGAAG+AGG + Chr5:235870-235889 None:intergenic 60.0%
! TCGTGTTTGGGGGGTGTAGG+AGG + Chr5:236079-236098 None:intergenic 60.0%
ATCTCACGGCGGCTCCGACT+CGG + Chr5:235745-235764 None:intergenic 65.0%
CCTGGAATGCCAGTGCAGGG+TGG - Chr5:236181-236200 MsG0580024047.01.T01:intron 65.0%
GTCATCATCGGCGGTGGCAG+CGG - Chr5:235787-235806 MsG0580024047.01.T01:three_prime_UTR 65.0%
TAACAAAGGGGGGAGGGGGG+AGG + Chr5:237703-237722 None:intergenic 65.0%
TCGGAGCCGCCGTGAGATCA+TGG - Chr5:235747-235766 MsG0580024047.01.T01:three_prime_UTR 65.0%
! CCACCCTGCACTGGCATTCC+AGG + Chr5:236184-236203 None:intergenic 65.0%
! CCCTGCACTGGCATTCCAGG+AGG + Chr5:236181-236200 None:intergenic 65.0%
TCGGCGGTGGCAGCGGAAGA+TGG - Chr5:235794-235813 MsG0580024047.01.T01:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 235600 238548 235600 ID=MsG0580024047.01;Name=MsG0580024047.01
Chr5 mRNA 235600 238548 235600 ID=MsG0580024047.01.T01;Parent=MsG0580024047.01;Name=MsG0580024047.01.T01;_AED=0.25;_eAED=0.25;_QI=134|0.66|0.75|1|1|1|4|216|387
Chr5 exon 235600 236178 235600 ID=MsG0580024047.01.T01:exon:23059;Parent=MsG0580024047.01.T01
Chr5 exon 236299 236345 236299 ID=MsG0580024047.01.T01:exon:23058;Parent=MsG0580024047.01.T01
Chr5 exon 237543 237636 237543 ID=MsG0580024047.01.T01:exon:23057;Parent=MsG0580024047.01.T01
Chr5 exon 237755 238548 237755 ID=MsG0580024047.01.T01:exon:23056;Parent=MsG0580024047.01.T01
Chr5 five_prime_UTR 238415 238548 238415 ID=MsG0580024047.01.T01:five_prime_utr;Parent=MsG0580024047.01.T01
Chr5 CDS 237755 238414 237755 ID=MsG0580024047.01.T01:cds;Parent=MsG0580024047.01.T01
Chr5 CDS 237543 237636 237543 ID=MsG0580024047.01.T01:cds;Parent=MsG0580024047.01.T01
Chr5 CDS 236299 236345 236299 ID=MsG0580024047.01.T01:cds;Parent=MsG0580024047.01.T01
Chr5 CDS 235816 236178 235816 ID=MsG0580024047.01.T01:cds;Parent=MsG0580024047.01.T01
Chr5 three_prime_UTR 235600 235815 235600 ID=MsG0580024047.01.T01:three_prime_utr;Parent=MsG0580024047.01.T01
Gene Sequence

>MsG0580024047.01.T01

ATGTATGTCCGAGTCGGAGCCGCCGTGAGATCATGGAGTCGTCGTCGTCAGTTGTCATCATCGGCGGTGGCAGCGGAAGATGGAAAATGGCTTACACTGCCACCTGTGAGTACTACTACTGTAAACCAAGTGACCTCTTCGACTTCGACTCCTGCCCTGAAATGGGTTATACGATGTTGCCCTCAACTACCTAAAACCCTTATCCATAAACTCTTTCGTCTAAAACAGGTTCGAATGCTCCCACAACAACAACATGATAACAAGTTCAAAAGGGTTACACCTAAGGATACTTTGAACTCAGGAGATCGTATATTTCTTCCCAATTCTGTTAAACAACAAGTTCCTCCTACACCCCCCAAACACGAATCTCTCACTGCTAAACAAATAAACTTCATTCGTTCTCTTGTTATCTATAAATATCCTCACATCCTTGTTCTAAACAAACCTCCTGGAATGCCAGTGCAGGGTGGCATTAATATCAAACGGAGTTTAGATTCTGTTGCTGCTGAATGTTTAAATTATGATTACTCTCAACCTCCTCGTTTGGTGCATAGACTAGACAGAGATTGTTCTGGAATTCTTGTCATGGGAAGAACAAAGACAAGCACCGCACTTTTGCATTCCATCTTCCGTGAAAAAACATCAAGGGCTTCAAATGAGAATGACACTGAAAAGAGAATCCTACAAAGACGGTATTGGGCATTGGTTCTTGGATGTCCTAGACGTTCAGGCGGAGTCATTAATGCTCCACTGGGTTATACATGGTTGGAGCTTTCCCCTTTAACCGGTAGAAAACACCAGCTCCGTGTCCACTGTGCTGAAGTGTTAGGTACACCGATAATTGGGGACTACAAGTATGGACGGCAAGCTCATAAGAAGTGGGGACAGTTTGATTTGTCTAATCCGGAGGACTCAACTGAAGAACATCTCAAGGAAGAGGCACTCCCTTTTGGCCTTAATTTGAATAAGGGGAGCATCTCTGACAAACATCCTCGATTACATCTTCATTGCAAGCAAATAGTCTTGCCCAATATATCTCAAGCACTGCAAAATGTGCAATCAGTATCAGATTCAAGTTATGACCTCTCAGTAGTTGAATCACTTGAGCTGGAAGCGGATTTGCCCCCATACATGAAAAAGAGTTGGGAGGTGACAAATTCTTGA
Protein sequence

>MsG0580024047.01.T01

MYVRVGAAVRSWSRRRQLSSSAVAAEDGKWLTLPPVSTTTVNQVTSSTSTPALKWVIRCCPQLPKTLIHKLFRLKQVRMLPQQQHDNKFKRVTPKDTLNSGDRIFLPNSVKQQVPPTPPKHESLTAKQINFIRSLVIYKYPHILVLNKPPGMPVQGGINIKRSLDSVAAECLNYDYSQPPRLVHRLDRDCSGILVMGRTKTSTALLHSIFREKTSRASNENDTEKRILQRRYWALVLGCPRRSGGVINAPLGYTWLELSPLTGRKHQLRVHCAEVLGTPIIGDYKYGRQAHKKWGQFDLSNPEDSTEEHLKEEALPFGLNLNKGSISDKHPRLHLHCKQIVLPNISQALQNVQSVSDSSYDLSVVESLELEADLPPYMKKSWEVTNS*