AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024064.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024064.01.T01 MTR_5g005050 73.121 346 32 8 1 286 1 345 8.65e-169 471
MsG0580024064.01.T01 MTR_5g005050 92.958 213 9 2 1 208 1 212 3.63e-143 407
MsG0580024064.01.T01 MTR_5g005050 96.386 83 3 0 204 286 298 380 2.44e-47 162
MsG0580024064.01.T01 MTR_8g064490 35.023 217 101 8 11 213 92 282 2.14e-27 110
MsG0580024064.01.T01 MTR_5g079860 39.869 153 77 5 82 232 117 256 4.16e-22 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024064.01.T01 AT3G19630 71.154 208 55 3 1 208 1 203 3.82e-96 288
MsG0580024064.01.T01 AT3G19630 64.286 84 29 1 204 286 289 372 8.07e-27 108
MsG0580024064.01.T01 AT1G60230 35.500 200 94 7 20 207 113 289 2.93e-25 104
MsG0580024064.01.T01 AT2G39670 44.538 119 60 5 116 232 163 277 6.37e-19 86.7
MsG0580024064.01.T01 AT2G39670 44.538 119 60 5 116 232 166 280 6.52e-19 86.7

Find 74 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCAGGTTGGATGCAAAA+TGG 0.233494 5:+391593 MsG0580024064.01.T01:CDS
CAAAGTTCATTCCAATTATA+TGG 0.237394 5:+391286 MsG0580024064.01.T01:CDS
AAGAGATGCTTCCATATAAT+TGG 0.240823 5:-391297 None:intergenic
TGATATTATGTATTCATATA+GGG 0.246938 5:+393393 MsG0580024064.01.T01:exon
TTGATATTATGTATTCATAT+AGG 0.276944 5:+393392 MsG0580024064.01.T01:exon
ATTCTGTGCAACTGGAAGTA+TGG 0.286571 5:+391623 MsG0580024064.01.T01:CDS
TTAATACCTTTCAACTCTAT+TGG 0.301051 5:+393165 MsG0580024064.01.T01:CDS
TTGAAGCTTTATCACAAGTT+TGG 0.321874 5:-391466 None:intergenic
GATACTCGTTTGGGTAAATA+TGG 0.323543 5:+391525 MsG0580024064.01.T01:CDS
GACTGTTGTTCGAATATTAT+AGG 0.343108 5:-393256 None:intergenic
TACTTTATGTATTTCTTCTC+AGG 0.346947 5:+391578 MsG0580024064.01.T01:CDS
CATATAATTGGAATGAACTT+TGG 0.355589 5:-391285 None:intergenic
TATCCGTAATGTTGTCTTCA+TGG 0.357605 5:+391713 MsG0580024064.01.T01:CDS
TATAGGGTTAATTTAACTCT+TGG 0.366687 5:+393409 MsG0580024064.01.T01:three_prime_UTR
GAAGAAACAATAATTCTGAT+TGG 0.377330 5:+391322 MsG0580024064.01.T01:CDS
GAATCTGTGCGCATCATGAC+TGG 0.379358 5:+391857 MsG0580024064.01.T01:CDS
TGTCCTCACCCATCTGCTTC+CGG 0.386591 5:-393278 None:intergenic
ATAGGGTTAATTTAACTCTT+GGG 0.396162 5:+393410 MsG0580024064.01.T01:three_prime_UTR
TATGAGATATGATACTCGTT+TGG 0.402274 5:+391515 MsG0580024064.01.T01:CDS
ATGAGATATGATACTCGTTT+GGG 0.407618 5:+391516 MsG0580024064.01.T01:CDS
TCGATTGCCGCCGCCGAGGA+TGG 0.407971 5:-391180 None:intergenic
GGTTGTAAATTCTGTGCAAC+TGG 0.417325 5:+391615 MsG0580024064.01.T01:CDS
AAGATCTTCTATATCCGTTA+GGG 0.419980 5:-393364 None:intergenic
CAAGATCTTCTATATCCGTT+AGG 0.423990 5:-393365 None:intergenic
TCTCAGGTTGGATGCAAAAT+GGG 0.425113 5:+391594 MsG0580024064.01.T01:CDS
CATTTCAGTTGTCATTGAAA+AGG 0.427528 5:+391885 MsG0580024064.01.T01:CDS
AAGAAACAATAATTCTGATT+GGG 0.429002 5:+391323 MsG0580024064.01.T01:CDS
CGATTGCCGCCGCCGAGGAT+GGG 0.445450 5:-391179 None:intergenic
TTATGTATTTCTTCTCAGGT+TGG 0.448424 5:+391582 MsG0580024064.01.T01:CDS
ACAATAATTCTGATTGGGAA+TGG 0.456253 5:+391328 MsG0580024064.01.T01:CDS
CAATAATTCTGATTGGGAAT+GGG 0.463962 5:+391329 MsG0580024064.01.T01:CDS
ACCAAAGATCATTCGAATCA+AGG 0.467982 5:-393548 None:intergenic
TTCTGTGCAACTGGAAGTAT+GGG 0.477827 5:+391624 MsG0580024064.01.T01:CDS
ATGAAGAGAAGAAGAGAGAG+GGG 0.513472 5:-391412 None:intergenic
AAGTGGTGCGTGCGGACAAT+TGG 0.513561 5:+393302 MsG0580024064.01.T01:CDS
TTTAAAAGTAACTTATCCTC+TGG 0.519468 5:+391648 MsG0580024064.01.T01:CDS
TTACCATGAAGACAACATTA+CGG 0.521555 5:-391716 None:intergenic
GCATTTCCGAGAGACTTGTC+AGG 0.526946 5:-393336 None:intergenic
AATGAAGAGAAGAAGAGAGA+GGG 0.528611 5:-391413 None:intergenic
TAAGCTGAAGAAGGTAAAGA+AGG 0.531806 5:-391363 None:intergenic
GATAACCCTCCTCCCATCCT+CGG 0.535896 5:+391167 None:intergenic
TGGTGCGTGCGGACAATTGG+TGG 0.546775 5:+393305 MsG0580024064.01.T01:CDS
CCTCCTCCCATCCTCGGCGG+CGG 0.559018 5:+391173 None:intergenic
AGGAATGAATAAGCTGAAGA+AGG 0.563073 5:-391372 None:intergenic
AACAACAGTCCGGAAGCAGA+TGG 0.563846 5:+393269 MsG0580024064.01.T01:CDS
AACTTACCTGACAAGTCTCT+CGG 0.568451 5:+393330 MsG0580024064.01.T01:CDS
AGTCCGGAAGCAGATGGGTG+AGG 0.575221 5:+393275 MsG0580024064.01.T01:CDS
TGGTGAAATTGTCGAGCAGT+TGG 0.575340 5:+391668 MsG0580024064.01.T01:CDS
GAATGAAGAGAAGAAGAGAG+AGG 0.577552 5:-391414 None:intergenic
CTTGTGATAAAGCTTCAAAA+CGG 0.582261 5:+391471 MsG0580024064.01.T01:CDS
TATGGTGGTCAACCTCGTCC+TGG 0.584796 5:+391543 MsG0580024064.01.T01:CDS
AATGTACCAATAGAGTTGAA+AGG 0.587561 5:-393171 None:intergenic
ATAATATTCGAACAACAGTC+CGG 0.589378 5:+393259 MsG0580024064.01.T01:CDS
GAAAAGGATTACCGTCTCAA+CGG 0.598039 5:+391901 MsG0580024064.01.T01:CDS
AAAATCCGATCGGTATTTGA+CGG 0.603787 5:+391222 MsG0580024064.01.T01:CDS
AAAGTAGCTCTCAGACCACC+AGG 0.606040 5:-391561 None:intergenic
ACAACAGTCCGGAAGCAGAT+GGG 0.608173 5:+393270 MsG0580024064.01.T01:CDS
GATCTTCTATATCCGTTAGG+GGG 0.613539 5:-393362 None:intergenic
ACAGCAGAATAGTTGTTCAA+AGG 0.614724 5:-391830 None:intergenic
AATCTGTGCGCATCATGACT+GGG 0.616777 5:+391858 MsG0580024064.01.T01:CDS
AAGTGACGTTATCAGAAGAG+TGG 0.616950 5:-391443 None:intergenic
GCTCTCAGACCACCAGGACG+AGG 0.621474 5:-391555 None:intergenic
ACTTTCGATTGCCGCCGCCG+AGG 0.622533 5:-391184 None:intergenic
TTTGAACAACTATTCTGCTG+TGG 0.630303 5:+391832 MsG0580024064.01.T01:CDS
ACTCGTTTGGGTAAATATGG+TGG 0.633657 5:+391528 MsG0580024064.01.T01:CDS
AGATCTTCTATATCCGTTAG+GGG 0.635379 5:-393363 None:intergenic
GGTGGTCAACCTCGTCCTGG+TGG 0.653114 5:+391546 MsG0580024064.01.T01:CDS
CGACGCCGTCAAATACCGAT+CGG 0.655834 5:-391227 None:intergenic
GAGAAGAATGAAAATCCGAT+CGG 0.664347 5:+391212 MsG0580024064.01.T01:exon
CAGATGGGTGAGGACATAAG+TGG 0.670930 5:+393285 MsG0580024064.01.T01:CDS
GAGGACATAAGTGGTGCGTG+CGG 0.673751 5:+393294 MsG0580024064.01.T01:CDS
TCAAAACAATACATAAAACA+AGG 0.675173 5:-393454 None:intergenic
AACCCTCCTCCCATCCTCGG+CGG 0.681297 5:+391170 None:intergenic
CTGCTCGACAATTTCACCAG+AGG 0.696718 5:-391664 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATATTATGTATTCATATA+GGG + Chr5:393393-393412 MsG0580024064.01.T01:exon 15.0%
!! TTAATAAAGAAGATATGATT+TGG - Chr5:392022-392041 None:intergenic 15.0%
!! TTGATATTATGTATTCATAT+AGG + Chr5:393392-393411 MsG0580024064.01.T01:exon 15.0%
!!! AAAACTTTAAAATGTGTTTT+TGG - Chr5:392169-392188 None:intergenic 15.0%
!!! TAAGTTTCAGAAAATTTTAA+GGG + Chr5:393230-393249 MsG0580024064.01.T01:CDS 15.0%
!! AAGAAACAATAATTCTGATT+GGG + Chr5:391323-391342 MsG0580024064.01.T01:CDS 20.0%
!! AGATAAGTTAATTATGTGTT+TGG - Chr5:392344-392363 None:intergenic 20.0%
!! TCAAAACAATACATAAAACA+AGG - Chr5:393457-393476 None:intergenic 20.0%
!!! AAGTTTCAGAAAATTTTAAG+GGG + Chr5:393231-393250 MsG0580024064.01.T01:CDS 20.0%
!!! ACATTTTAAAGTTTTTCAAG+TGG + Chr5:392174-392193 MsG0580024064.01.T01:intron 20.0%
!!! CTAAGTTTCAGAAAATTTTA+AGG + Chr5:393229-393248 MsG0580024064.01.T01:CDS 20.0%
!!! TTTTTATTATTACAGGGAAT+GGG + Chr5:391803-391822 MsG0580024064.01.T01:intron 20.0%
!!! TTTTTTATTATTACAGGGAA+TGG + Chr5:391802-391821 MsG0580024064.01.T01:intron 20.0%
! ATAGGGTTAATTTAACTCTT+GGG + Chr5:393410-393429 MsG0580024064.01.T01:three_prime_UTR 25.0%
! ATTGAATACATAATGCTTGA+TGG + Chr5:392865-392884 MsG0580024064.01.T01:intron 25.0%
! CAAAGTTCATTCCAATTATA+TGG + Chr5:391286-391305 MsG0580024064.01.T01:CDS 25.0%
! CATATAATTGGAATGAACTT+TGG - Chr5:391288-391307 None:intergenic 25.0%
! GAAGAAACAATAATTCTGAT+TGG + Chr5:391322-391341 MsG0580024064.01.T01:CDS 25.0%
! TACTTCAAAATTCAAACTTC+AGG - Chr5:392997-393016 None:intergenic 25.0%
! TACTTTATGTATTTCTTCTC+AGG + Chr5:391578-391597 MsG0580024064.01.T01:CDS 25.0%
! TATAGGGTTAATTTAACTCT+TGG + Chr5:393409-393428 MsG0580024064.01.T01:three_prime_UTR 25.0%
! TCTTGAATGAAAGAAATTTC+TGG + Chr5:392534-392553 MsG0580024064.01.T01:intron 25.0%
! TTAATACCTTTCAACTCTAT+TGG + Chr5:393165-393184 MsG0580024064.01.T01:CDS 25.0%
! TTTAAAAGTAACTTATCCTC+TGG + Chr5:391648-391667 MsG0580024064.01.T01:CDS 25.0%
!! AGAACTTTTACCAAAAGAAA+TGG - Chr5:392517-392536 None:intergenic 25.0%
AAGAGATGCTTCCATATAAT+TGG - Chr5:391300-391319 None:intergenic 30.0%
AAGATCTTCTATATCCGTTA+GGG - Chr5:393367-393386 None:intergenic 30.0%
AATGTACCAATAGAGTTGAA+AGG - Chr5:393174-393193 None:intergenic 30.0%
ACAATAATTCTGATTGGGAA+TGG + Chr5:391328-391347 MsG0580024064.01.T01:CDS 30.0%
ATAATATTCGAACAACAGTC+CGG + Chr5:393259-393278 MsG0580024064.01.T01:CDS 30.0%
ATACTTGATCATAAGCTCTT+GGG - Chr5:392318-392337 None:intergenic 30.0%
ATGAGATATGATACTCGTTT+GGG + Chr5:391516-391535 MsG0580024064.01.T01:CDS 30.0%
CAATAATTCTGATTGGGAAT+GGG + Chr5:391329-391348 MsG0580024064.01.T01:CDS 30.0%
CATTTCAGTTGTCATTGAAA+AGG + Chr5:391885-391904 MsG0580024064.01.T01:CDS 30.0%
CATTTGCATCTATAATAGGT+TGG + Chr5:392570-392589 MsG0580024064.01.T01:intron 30.0%
CTCAAATAGTAGTAATTGCT+AGG - Chr5:392045-392064 None:intergenic 30.0%
CTTGTGATAAAGCTTCAAAA+CGG + Chr5:391471-391490 MsG0580024064.01.T01:CDS 30.0%
GACTGTTGTTCGAATATTAT+AGG - Chr5:393259-393278 None:intergenic 30.0%
GGTACATTTGCATCTATAAT+AGG + Chr5:392566-392585 MsG0580024064.01.T01:intron 30.0%
TAGAAGACAATGCAAGTAAA+TGG - Chr5:391938-391957 None:intergenic 30.0%
TATGAGATATGATACTCGTT+TGG + Chr5:391515-391534 MsG0580024064.01.T01:CDS 30.0%
TGCAAGAATATCAAAGGAAA+AGG + Chr5:392736-392755 MsG0580024064.01.T01:intron 30.0%
TTACCATGAAGACAACATTA+CGG - Chr5:391719-391738 None:intergenic 30.0%
TTATGTATTTCTTCTCAGGT+TGG + Chr5:391582-391601 MsG0580024064.01.T01:CDS 30.0%
! CATATGAAAAATCCTTTTCC+TGG - Chr5:392400-392419 None:intergenic 30.0%
! CATTGTTAAGCCATTTCTTT+TGG + Chr5:392504-392523 MsG0580024064.01.T01:intron 30.0%
! CTTTTCAATGACAACTGAAA+TGG - Chr5:391887-391906 None:intergenic 30.0%
! TAAAGAAGATATGATTTGGC+TGG - Chr5:392018-392037 None:intergenic 30.0%
! TTGAAGCTTTATCACAAGTT+TGG - Chr5:391469-391488 None:intergenic 30.0%
! TTGATATTTTACCGTTGAGA+CGG - Chr5:391915-391934 None:intergenic 30.0%
! TTTCCCTTTGGAAAAACTAA+TGG + Chr5:392707-392726 MsG0580024064.01.T01:intron 30.0%
!! AAGAAATTTCTGGCTTATGA+AGG + Chr5:392544-392563 MsG0580024064.01.T01:intron 30.0%
!! AGAAATTTCTGGCTTATGAA+GGG + Chr5:392545-392564 MsG0580024064.01.T01:intron 30.0%
!! AGTAGAAGTAAAAAAGCACT+TGG + Chr5:393500-393519 MsG0580024064.01.T01:three_prime_UTR 30.0%
!! GATACCCATTTTTGAGAATT+CGG - Chr5:391262-391281 None:intergenic 30.0%
!!! AAAGTTTTTCAAGTGGTGTT+TGG + Chr5:392181-392200 MsG0580024064.01.T01:intron 30.0%
!!! AAGCCATTAGTTTTTCCAAA+GGG - Chr5:392713-392732 None:intergenic 30.0%
AAAATCCGATCGGTATTTGA+CGG + Chr5:391222-391241 MsG0580024064.01.T01:CDS 35.0%
AATGAAGAGAAGAAGAGAGA+GGG - Chr5:391416-391435 None:intergenic 35.0%
ACAGCAGAATAGTTGTTCAA+AGG - Chr5:391833-391852 None:intergenic 35.0%
AGATCTTCTATATCCGTTAG+GGG - Chr5:393366-393385 None:intergenic 35.0%
AGGAATGAATAAGCTGAAGA+AGG - Chr5:391375-391394 None:intergenic 35.0%
CAAGATCTTCTATATCCGTT+AGG - Chr5:393368-393387 None:intergenic 35.0%
CGAACCGAATTCTCAAAAAT+GGG + Chr5:391255-391274 MsG0580024064.01.T01:CDS 35.0%
CTTCACTGCAAGAATATCAA+AGG + Chr5:392730-392749 MsG0580024064.01.T01:intron 35.0%
GACATTAACAACAGGTCTTA+GGG + Chr5:392121-392140 MsG0580024064.01.T01:intron 35.0%
GAGAAGAATGAAAATCCGAT+CGG + Chr5:391212-391231 MsG0580024064.01.T01:exon 35.0%
GATACTCGTTTGGGTAAATA+TGG + Chr5:391525-391544 MsG0580024064.01.T01:CDS 35.0%
GATACTTGATCATAAGCTCT+TGG - Chr5:392319-392338 None:intergenic 35.0%
TAAGCTGAAGAAGGTAAAGA+AGG - Chr5:391366-391385 None:intergenic 35.0%
TACTTGATCATAAGCTCTTG+GGG - Chr5:392317-392336 None:intergenic 35.0%
TATCCGTAATGTTGTCTTCA+TGG + Chr5:391713-391732 MsG0580024064.01.T01:CDS 35.0%
TGTTGTCTGAATTGTTATGC+AGG + Chr5:393134-393153 MsG0580024064.01.T01:intron 35.0%
TTGTTAATGTCGCAAACTCT+TGG - Chr5:392112-392131 None:intergenic 35.0%
TTTGAACAACTATTCTGCTG+TGG + Chr5:391832-391851 MsG0580024064.01.T01:CDS 35.0%
! GTGAACAGTTTCAGTGATTT+TGG + Chr5:392808-392827 MsG0580024064.01.T01:intron 35.0%
!! TCTAATGCGAAAGCTAGTTT+TGG - Chr5:392373-392392 None:intergenic 35.0%
!!! GAAGCCATTAGTTTTTCCAA+AGG - Chr5:392714-392733 None:intergenic 35.0%
AACTTACCTGACAAGTCTCT+CGG + Chr5:393330-393349 MsG0580024064.01.T01:CDS 40.0%
AAGTGACGTTATCAGAAGAG+TGG - Chr5:391446-391465 None:intergenic 40.0%
ACTCGTTTGGGTAAATATGG+TGG + Chr5:391528-391547 MsG0580024064.01.T01:CDS 40.0%
AGAATCATGCAGAAGAGCTT+AGG + Chr5:392237-392256 MsG0580024064.01.T01:intron 40.0%
ATGAAGAGAAGAAGAGAGAG+GGG - Chr5:391415-391434 None:intergenic 40.0%
ATTCTGTGCAACTGGAAGTA+TGG + Chr5:391623-391642 MsG0580024064.01.T01:CDS 40.0%
CGACATTAACAACAGGTCTT+AGG + Chr5:392120-392139 MsG0580024064.01.T01:intron 40.0%
GAAAAGGATTACCGTCTCAA+CGG + Chr5:391901-391920 MsG0580024064.01.T01:CDS 40.0%
GAATGAAGAGAAGAAGAGAG+AGG - Chr5:391417-391436 None:intergenic 40.0%
GAGTTTGCGACATTAACAAC+AGG + Chr5:392113-392132 MsG0580024064.01.T01:intron 40.0%
GATCTTCTATATCCGTTAGG+GGG - Chr5:393365-393384 None:intergenic 40.0%
GCATTAGAACTTCCAGGAAA+AGG + Chr5:392385-392404 MsG0580024064.01.T01:intron 40.0%
GCGAACCGAATTCTCAAAAA+TGG + Chr5:391254-391273 MsG0580024064.01.T01:CDS 40.0%
GCTTCTCTATTCTCAACCAA+TGG + Chr5:392283-392302 MsG0580024064.01.T01:intron 40.0%
GGTTGTAAATTCTGTGCAAC+TGG + Chr5:391615-391634 MsG0580024064.01.T01:CDS 40.0%
TGAAATCTGAGAATTCCCTG+AGG + Chr5:392065-392084 MsG0580024064.01.T01:intron 40.0%
TTTAATAGCACTGCGCAACA+AGG - Chr5:393106-393125 None:intergenic 40.0%
! ACATAATCTGCATTAGCCTC+AGG - Chr5:392084-392103 None:intergenic 40.0%
! CATAATCTGCATTAGCCTCA+GGG - Chr5:392083-392102 None:intergenic 40.0%
! GTTTTTCCAAAGGGAAAGCA+CGG - Chr5:392704-392723 None:intergenic 40.0%
! TTCTGTGCAACTGGAAGTAT+GGG + Chr5:391624-391643 MsG0580024064.01.T01:CDS 40.0%
!! TCTCAGGTTGGATGCAAAAT+GGG + Chr5:391594-391613 MsG0580024064.01.T01:CDS 40.0%
!! TTCTCAGGTTGGATGCAAAA+TGG + Chr5:391593-391612 MsG0580024064.01.T01:CDS 40.0%
!! TTTTTCCAAAGGGAAAGCAC+GGG - Chr5:392703-392722 None:intergenic 40.0%
!!! GAGGGGTTTTGAAGTTTGAA+CGG - Chr5:391398-391417 None:intergenic 40.0%
AAGCTTCATAATGACGTGCC+TGG + Chr5:392612-392631 MsG0580024064.01.T01:intron 45.0%
AATCTGTGCGCATCATGACT+GGG + Chr5:391858-391877 MsG0580024064.01.T01:CDS 45.0%
CATGCAGAAGAGCTTAGGTT+AGG + Chr5:392242-392261 MsG0580024064.01.T01:intron 45.0%
GCTTTCGCATTAGAACTTCC+AGG + Chr5:392379-392398 MsG0580024064.01.T01:intron 45.0%
TATCTGGCAACGGATGTCTT+GGG - Chr5:392670-392689 None:intergenic 45.0%
TGGTGAAATTGTCGAGCAGT+TGG + Chr5:391668-391687 MsG0580024064.01.T01:CDS 45.0%
TTATCTGGCAACGGATGTCT+TGG - Chr5:392671-392690 None:intergenic 45.0%
!!! GGGTTTTGAAGTTTGAACGG+AGG - Chr5:391395-391414 None:intergenic 45.0%
!!! TTTTTTTTTTTATTATTACA+GGG + Chr5:391797-391816 MsG0580024064.01.T01:intron 5.0%
!!! TTTTTTTTTTTTATTATTAC+AGG + Chr5:391796-391815 MsG0580024064.01.T01:intron 5.0%
AAAGTAGCTCTCAGACCACC+AGG - Chr5:391564-391583 None:intergenic 50.0%
AACAACAGTCCGGAAGCAGA+TGG + Chr5:393269-393288 MsG0580024064.01.T01:CDS 50.0%
ACAACAGTCCGGAAGCAGAT+GGG + Chr5:393270-393289 MsG0580024064.01.T01:CDS 50.0%
CAGATGGGTGAGGACATAAG+TGG + Chr5:393285-393304 MsG0580024064.01.T01:CDS 50.0%
CAGCAGGCATTATCTGGCAA+CGG - Chr5:392680-392699 None:intergenic 50.0%
CGGAAATGCTAACCCCCTAA+CGG + Chr5:393350-393369 MsG0580024064.01.T01:CDS 50.0%
CTGCTCGACAATTTCACCAG+AGG - Chr5:391667-391686 None:intergenic 50.0%
GAATCTGTGCGCATCATGAC+TGG + Chr5:391857-391876 MsG0580024064.01.T01:CDS 50.0%
GAGACTGCCAAGTTCAAACC+AGG - Chr5:392633-392652 None:intergenic 50.0%
GCAGAAGAGCTTAGGTTAGG+TGG + Chr5:392245-392264 MsG0580024064.01.T01:intron 50.0%
GCATTTCCGAGAGACTTGTC+AGG - Chr5:393339-393358 None:intergenic 50.0%
GCTGCTGGAGACATTTGAAG+TGG + Chr5:392926-392945 MsG0580024064.01.T01:intron 50.0%
TCTCCAGCAGCTTTCCTAGT+AGG - Chr5:392917-392936 None:intergenic 50.0%
TCTTGGGGAAGCTGTTCCAT+TGG - Chr5:392302-392321 None:intergenic 50.0%
! TGACGTGCCTGGTTTGAACT+TGG + Chr5:392623-392642 MsG0580024064.01.T01:intron 50.0%
AAGTGGTGCGTGCGGACAAT+TGG + Chr5:393302-393321 MsG0580024064.01.T01:CDS 55.0%
AGAGCAGCATGCCACCTACT+AGG + Chr5:392900-392919 MsG0580024064.01.T01:intron 55.0%
CCACCTACTAGGAAAGCTGC+TGG + Chr5:392911-392930 MsG0580024064.01.T01:intron 55.0%
CCAGCAGCTTTCCTAGTAGG+TGG - Chr5:392914-392933 None:intergenic 55.0%
CGACGCCGTCAAATACCGAT+CGG - Chr5:391230-391249 None:intergenic 55.0%
GAGGACATAAGTGGTGCGTG+CGG + Chr5:393294-393313 MsG0580024064.01.T01:CDS 55.0%
TATGGTGGTCAACCTCGTCC+TGG + Chr5:391543-391562 MsG0580024064.01.T01:CDS 55.0%
TGTCCTCACCCATCTGCTTC+CGG - Chr5:393281-393300 None:intergenic 55.0%
AGTCCGGAAGCAGATGGGTG+AGG + Chr5:393275-393294 MsG0580024064.01.T01:CDS 60.0%
CACGGGCAGCAGGCATTATC+TGG - Chr5:392686-392705 None:intergenic 60.0%
TGCTGCCCGTGCTTTCCCTT+TGG + Chr5:392695-392714 MsG0580024064.01.T01:intron 60.0%
TGGCAACGGATGTCTTGGGC+TGG - Chr5:392666-392685 None:intergenic 60.0%
TGGTGCGTGCGGACAATTGG+TGG + Chr5:393305-393324 MsG0580024064.01.T01:CDS 60.0%
! AAAGGGAAAGCACGGGCAGC+AGG - Chr5:392696-392715 None:intergenic 60.0%
ACTTTCGATTGCCGCCGCCG+AGG - Chr5:391187-391206 None:intergenic 65.0%
GCTCTCAGACCACCAGGACG+AGG - Chr5:391558-391577 None:intergenic 65.0%
GGTGGTCAACCTCGTCCTGG+TGG + Chr5:391546-391565 MsG0580024064.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 391182 393557 391182 ID=MsG0580024064.01;Name=MsG0580024064.01
Chr5 mRNA 391182 393557 391182 ID=MsG0580024064.01.T01;Parent=MsG0580024064.01;Name=MsG0580024064.01.T01;_AED=0.30;_eAED=0.30;_QI=37|0.5|1|1|1|1|3|162|286
Chr5 exon 391182 391734 391182 ID=MsG0580024064.01.T01:exon:97;Parent=MsG0580024064.01.T01
Chr5 exon 391818 391922 391818 ID=MsG0580024064.01.T01:exon:98;Parent=MsG0580024064.01.T01
Chr5 exon 393156 393557 393156 ID=MsG0580024064.01.T01:exon:99;Parent=MsG0580024064.01.T01
Chr5 five_prime_UTR 391182 391218 391182 ID=MsG0580024064.01.T01:five_prime_utr;Parent=MsG0580024064.01.T01
Chr5 CDS 391219 391734 391219 ID=MsG0580024064.01.T01:cds;Parent=MsG0580024064.01.T01
Chr5 CDS 391818 391922 391818 ID=MsG0580024064.01.T01:cds;Parent=MsG0580024064.01.T01
Chr5 CDS 393156 393395 393156 ID=MsG0580024064.01.T01:cds;Parent=MsG0580024064.01.T01
Chr5 three_prime_UTR 393396 393557 393396 ID=MsG0580024064.01.T01:three_prime_utr;Parent=MsG0580024064.01.T01
Gene Sequence

>MsG0580024064.01.T01

ATGAAAATCCGATCGGTATTTGACGGCGTCGAGCTGCGAACCGAATTCTCAAAAATGGGTATCGATCCAAAGTTCATTCCAATTATATGGAAGCATCTCTTTCGAAGAAACAATAATTCTGATTGGGAATGGGAAAAACACGTTCCTTCTTTACCTTCTTCAGCTTATTCATTCCTCCGTTCAAACTTCAAAACCCCTCTCTCTTCTTCTCTTCATTCAGTTTTCCACTCTTCTGATAACGTCACTTCCAAACTTGTGATAAAGCTTCAAAACGGTGAATTTGTTGAAGCTGTGATTATGAGATATGATACTCGTTTGGGTAAATATGGTGGTCAACCTCGTCCTGGTGGTCTGAGAGCTACTTTATGTATTTCTTCTCAGGTTGGATGCAAAATGGGTTGTAAATTCTGTGCAACTGGAAGTATGGGATTTAAAAGTAACTTATCCTCTGGTGAAATTGTCGAGCAGTTGGTTCATGCTTCTACTTTTGCACATATCCGTAATGTTGTCTTCATGGGAATGGGAGAGCCTTTGAACAACTATTCTGCTGTGGTAGAATCTGTGCGCATCATGACTGGGTCACCATTTCAGTTGTCATTGAAAAGGATTACCGTCTCAACGGTAGTGAACTTAATACCTTTCAACTCTATTGGTACATTGAGTCAATTCAAATCTACTAGTGAGCAGAAAGTGTCTAAGTTTCAGAAAATTTTAAGGGGTACCTATAATATTCGAACAACAGTCCGGAAGCAGATGGGTGAGGACATAAGTGGTGCGTGCGGACAATTGGTGGTTAACTTACCTGACAAGTCTCTCGGAAATGCTAACCCCCTAACGGATATAGAAGATCTTGTTATTTGA
Protein sequence

>MsG0580024064.01.T01

MKIRSVFDGVELRTEFSKMGIDPKFIPIIWKHLFRRNNNSDWEWEKHVPSLPSSAYSFLRSNFKTPLSSSLHSVFHSSDNVTSKLVIKLQNGEFVEAVIMRYDTRLGKYGGQPRPGGLRATLCISSQVGCKMGCKFCATGSMGFKSNLSSGEIVEQLVHASTFAHIRNVVFMGMGEPLNNYSAVVESVRIMTGSPFQLSLKRITVSTVVNLIPFNSIGTLSQFKSTSEQKVSKFQKILRGTYNIRTTVRKQMGEDISGACGQLVVNLPDKSLGNANPLTDIEDLVI*