Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024186.01.T01 | XP_003610782.1 | 97.826 | 138 | 3 | 0 | 1 | 138 | 1 | 138 | 1.05E-93 | 286 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024186.01.T01 | Q9LJ87 | 60.377 | 106 | 42 | 0 | 8 | 113 | 1 | 106 | 8.29E-45 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024186.01.T01 | B7FK10 | 97.826 | 138 | 3 | 0 | 1 | 138 | 1 | 138 | 5.04e-94 | 286 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002716.01 | MsG0580024186.01 | 0.817239 | 3.655945e-52 | 2.647920e-49 |
| MsG0380015212.01 | MsG0580024186.01 | 0.825011 | 5.914121e-54 | 5.325931e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024186.01.T01 | MTR_5g006960 | 97.826 | 138 | 3 | 0 | 1 | 138 | 1 | 138 | 1.28e-97 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024186.01.T01 | AT3G22590 | 60.377 | 106 | 42 | 0 | 8 | 113 | 1 | 106 | 8.45e-46 | 154 |
Find 43 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGATAATTCATCTGAATT+AGG | 0.242358 | 5:+1712210 | None:intergenic |
| CTCCGTGATTTCACCATTAG+AGG | 0.266475 | 5:-1712567 | MsG0580024186.01.T01:CDS |
| TCGGGGATCGAAGGCTGTTC+GGG | 0.270432 | 5:+1712267 | None:intergenic |
| ATCGGGGATCGAAGGCTGTT+CGG | 0.335598 | 5:+1712266 | None:intergenic |
| CCAATTCTTAACTATCTTCA+AGG | 0.358227 | 5:-1712324 | MsG0580024186.01.T01:CDS |
| TTTCAAAATACTTTAGCTCT+TGG | 0.359318 | 5:-1712378 | MsG0580024186.01.T01:CDS |
| CCTTGAAGATAGTTAAGAAT+TGG | 0.360763 | 5:+1712324 | None:intergenic |
| GAAATCACGGAGGAGGGTTA+AGG | 0.375174 | 5:+1712578 | None:intergenic |
| CTCAACGGTAACTTCCGTTT+CGG | 0.409085 | 5:-1712522 | MsG0580024186.01.T01:CDS |
| TTTGTCTAAGTCGCCTCTAA+TGG | 0.409790 | 5:+1712554 | None:intergenic |
| GAAAATACTCGGCGTGTTTG+AGG | 0.447850 | 5:+1712397 | None:intergenic |
| GAGTGCTGTTGGAGAGAAGA+AGG | 0.449313 | 5:+1712240 | None:intergenic |
| TAGGAAATTGAGAGTGCTGT+TGG | 0.459018 | 5:+1712229 | None:intergenic |
| GACAAAATCGTCCGTCTCAA+CGG | 0.466820 | 5:-1712537 | MsG0580024186.01.T01:CDS |
| TGGAGAGAAGAAGGTTCATC+GGG | 0.475113 | 5:+1712249 | None:intergenic |
| AGTGTAGCGGTTTCCCTTTG+TGG | 0.475995 | 5:+1712455 | None:intergenic |
| AAATCACGGAGGAGGGTTAA+GGG | 0.487725 | 5:+1712579 | None:intergenic |
| ACAGCTTATCGCTCCACAAA+GGG | 0.505860 | 5:-1712468 | MsG0580024186.01.T01:CDS |
| GGGGAGATATTCAATTGAAT+CGG | 0.511278 | 5:+1712287 | None:intergenic |
| CAATTCTTAACTATCTTCAA+GGG | 0.535990 | 5:-1712323 | MsG0580024186.01.T01:CDS |
| AGGTGTAATCTTCGCCGAAA+CGG | 0.539346 | 5:+1712508 | None:intergenic |
| GAATCCGATGAGAATGAGAA+TGG | 0.539396 | 5:-1712604 | MsG0580024186.01.T01:CDS |
| TGGAGCGATAAGCTGTTTCG+AGG | 0.540395 | 5:+1712475 | None:intergenic |
| TAATGGTGAAATCACGGAGG+AGG | 0.550611 | 5:+1712571 | None:intergenic |
| TTGGAGAGAAGAAGGTTCAT+CGG | 0.553555 | 5:+1712248 | None:intergenic |
| TAAGCTGTTTCGAGGGAGCA+AGG | 0.557707 | 5:+1712483 | None:intergenic |
| CGATCAGGGAGGGTTACGGA+AGG | 0.560319 | 5:+1712351 | None:intergenic |
| CGCCTCTAATGGTGAAATCA+CGG | 0.562145 | 5:+1712565 | None:intergenic |
| GAAGGTTCATCGGGGATCGA+AGG | 0.564042 | 5:+1712258 | None:intergenic |
| ATATTCAATTGAATCGGTGG+TGG | 0.575653 | 5:+1712293 | None:intergenic |
| GGATCCATTCTCATTCTCAT+CGG | 0.580362 | 5:+1712600 | None:intergenic |
| GATCAGGGAGGGTTACGGAA+GGG | 0.584300 | 5:+1712352 | None:intergenic |
| AACAGCTTATCGCTCCACAA+AGG | 0.590351 | 5:-1712469 | MsG0580024186.01.T01:CDS |
| AAGCTGTTTCGAGGGAGCAA+GGG | 0.594853 | 5:+1712484 | None:intergenic |
| AATGGTGAAATCACGGAGGA+GGG | 0.595264 | 5:+1712572 | None:intergenic |
| GAGATATTCAATTGAATCGG+TGG | 0.607369 | 5:+1712290 | None:intergenic |
| AACGGAAGTTACCGTTGAGA+CGG | 0.627855 | 5:+1712526 | None:intergenic |
| CTAGGGTTTCGAGAGTGTAG+CGG | 0.634520 | 5:+1712442 | None:intergenic |
| TGTTTCGAGGGAGCAAGGGA+AGG | 0.637187 | 5:+1712488 | None:intergenic |
| CGGGGATCGAAGGCTGTTCG+GGG | 0.642909 | 5:+1712268 | None:intergenic |
| GGAGCGATAAGCTGTTTCGA+GGG | 0.662749 | 5:+1712476 | None:intergenic |
| CTCTAATGGTGAAATCACGG+AGG | 0.766855 | 5:+1712568 | None:intergenic |
| GGAGAGAAGAAGGTTCATCG+GGG | 0.783983 | 5:+1712250 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAGTATTTTGAAAATACT+CGG | + | Chr5:1712426-1712445 | None:intergenic | 15.0% |
| ! | AGTGATAATTCATCTGAATT+AGG | + | Chr5:1712602-1712621 | None:intergenic | 25.0% |
| ! | CAATTCTTAACTATCTTCAA+GGG | - | Chr5:1712486-1712505 | MsG0580024186.01.T01:CDS | 25.0% |
| !! | ATTTTTGATGTAGTGAACTA+GGG | + | Chr5:1712387-1712406 | None:intergenic | 25.0% |
| !!! | TTTCAAAATACTTTAGCTCT+TGG | - | Chr5:1712431-1712450 | MsG0580024186.01.T01:CDS | 25.0% |
| CCAATTCTTAACTATCTTCA+AGG | - | Chr5:1712485-1712504 | MsG0580024186.01.T01:CDS | 30.0% | |
| CCTTGAAGATAGTTAAGAAT+TGG | + | Chr5:1712488-1712507 | None:intergenic | 30.0% | |
| ! | GATTTTTGATGTAGTGAACT+AGG | + | Chr5:1712388-1712407 | None:intergenic | 30.0% |
| !!! | TAAGAATTGGTTTTCGATCA+GGG | + | Chr5:1712475-1712494 | None:intergenic | 30.0% |
| !!! | TTAAGAATTGGTTTTCGATC+AGG | + | Chr5:1712476-1712495 | None:intergenic | 30.0% |
| ATATTCAATTGAATCGGTGG+TGG | + | Chr5:1712519-1712538 | None:intergenic | 35.0% | |
| GAGATATTCAATTGAATCGG+TGG | + | Chr5:1712522-1712541 | None:intergenic | 35.0% | |
| GGGGAGATATTCAATTGAAT+CGG | + | Chr5:1712525-1712544 | None:intergenic | 35.0% | |
| GAATCCGATGAGAATGAGAA+TGG | - | Chr5:1712205-1712224 | MsG0580024186.01.T01:CDS | 40.0% | |
| GGATCCATTCTCATTCTCAT+CGG | + | Chr5:1712212-1712231 | None:intergenic | 40.0% | |
| TTGGAGAGAAGAAGGTTCAT+CGG | + | Chr5:1712564-1712583 | None:intergenic | 40.0% | |
| TTTGTCTAAGTCGCCTCTAA+TGG | + | Chr5:1712258-1712277 | None:intergenic | 40.0% | |
| !! | TAGGAAATTGAGAGTGCTGT+TGG | + | Chr5:1712583-1712602 | None:intergenic | 40.0% |
| !!! | AATTGGTTTTCGATCAGGGA+GGG | + | Chr5:1712471-1712490 | None:intergenic | 40.0% |
| AAATCACGGAGGAGGGTTAA+GGG | + | Chr5:1712233-1712252 | None:intergenic | 45.0% | |
| AACAGCTTATCGCTCCACAA+AGG | - | Chr5:1712340-1712359 | MsG0580024186.01.T01:CDS | 45.0% | |
| AACGGAAGTTACCGTTGAGA+CGG | + | Chr5:1712286-1712305 | None:intergenic | 45.0% | |
| AATGGTGAAATCACGGAGGA+GGG | + | Chr5:1712240-1712259 | None:intergenic | 45.0% | |
| ACAGCTTATCGCTCCACAAA+GGG | - | Chr5:1712341-1712360 | MsG0580024186.01.T01:CDS | 45.0% | |
| AGGTGTAATCTTCGCCGAAA+CGG | + | Chr5:1712304-1712323 | None:intergenic | 45.0% | |
| CGCCTCTAATGGTGAAATCA+CGG | + | Chr5:1712247-1712266 | None:intergenic | 45.0% | |
| CTCAACGGTAACTTCCGTTT+CGG | - | Chr5:1712287-1712306 | MsG0580024186.01.T01:CDS | 45.0% | |
| CTCCGTGATTTCACCATTAG+AGG | - | Chr5:1712242-1712261 | MsG0580024186.01.T01:CDS | 45.0% | |
| CTCTAATGGTGAAATCACGG+AGG | + | Chr5:1712244-1712263 | None:intergenic | 45.0% | |
| GAAAATACTCGGCGTGTTTG+AGG | + | Chr5:1712415-1712434 | None:intergenic | 45.0% | |
| GACAAAATCGTCCGTCTCAA+CGG | - | Chr5:1712272-1712291 | MsG0580024186.01.T01:CDS | 45.0% | |
| TAATGGTGAAATCACGGAGG+AGG | + | Chr5:1712241-1712260 | None:intergenic | 45.0% | |
| TGGAGAGAAGAAGGTTCATC+GGG | + | Chr5:1712563-1712582 | None:intergenic | 45.0% | |
| ! | TTTTCGATCAGGGAGGGTTA+CGG | + | Chr5:1712465-1712484 | None:intergenic | 45.0% |
| !!! | GAATTGGTTTTCGATCAGGG+AGG | + | Chr5:1712472-1712491 | None:intergenic | 45.0% |
| AGTGTAGCGGTTTCCCTTTG+TGG | + | Chr5:1712357-1712376 | None:intergenic | 50.0% | |
| GAAATCACGGAGGAGGGTTA+AGG | + | Chr5:1712234-1712253 | None:intergenic | 50.0% | |
| GGAGAGAAGAAGGTTCATCG+GGG | + | Chr5:1712562-1712581 | None:intergenic | 50.0% | |
| ! | AAGCTGTTTCGAGGGAGCAA+GGG | + | Chr5:1712328-1712347 | None:intergenic | 50.0% |
| ! | CTAGGGTTTCGAGAGTGTAG+CGG | + | Chr5:1712370-1712389 | None:intergenic | 50.0% |
| ! | GAGTGCTGTTGGAGAGAAGA+AGG | + | Chr5:1712572-1712591 | None:intergenic | 50.0% |
| ! | GGAGCGATAAGCTGTTTCGA+GGG | + | Chr5:1712336-1712355 | None:intergenic | 50.0% |
| ! | TAAGCTGTTTCGAGGGAGCA+AGG | + | Chr5:1712329-1712348 | None:intergenic | 50.0% |
| ! | TGGAGCGATAAGCTGTTTCG+AGG | + | Chr5:1712337-1712356 | None:intergenic | 50.0% |
| ATCGGGGATCGAAGGCTGTT+CGG | + | Chr5:1712546-1712565 | None:intergenic | 55.0% | |
| GAAGGTTCATCGGGGATCGA+AGG | + | Chr5:1712554-1712573 | None:intergenic | 55.0% | |
| GATCAGGGAGGGTTACGGAA+GGG | + | Chr5:1712460-1712479 | None:intergenic | 55.0% | |
| TGTTTCGAGGGAGCAAGGGA+AGG | + | Chr5:1712324-1712343 | None:intergenic | 55.0% | |
| CGATCAGGGAGGGTTACGGA+AGG | + | Chr5:1712461-1712480 | None:intergenic | 60.0% | |
| TCGGGGATCGAAGGCTGTTC+GGG | + | Chr5:1712545-1712564 | None:intergenic | 60.0% | |
| CGGGGATCGAAGGCTGTTCG+GGG | + | Chr5:1712544-1712563 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1712203 | 1712628 | 1712203 | ID=MsG0580024186.01;Name=MsG0580024186.01 |
| Chr5 | mRNA | 1712203 | 1712628 | 1712203 | ID=MsG0580024186.01.T01;Parent=MsG0580024186.01;Name=MsG0580024186.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|141 |
| Chr5 | exon | 1712203 | 1712628 | 1712203 | ID=MsG0580024186.01.T01:exon:491;Parent=MsG0580024186.01.T01 |
| Chr5 | CDS | 1712203 | 1712628 | 1712203 | ID=MsG0580024186.01.T01:cds;Parent=MsG0580024186.01.T01 |
| Gene Sequence |
| Protein sequence |
