AlfalfaGEDB | MsG0580025200.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025200.01

KEGG: -
GO: -
PFAM: PF02042

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025200.01.T01	XP_003612350.1	86.835	357	20	1	1	357	1	330	0	619

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025200.01.T01	O81791	46.067	356	135	10	1	340	40	354	3.97E-85	265

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025200.01.T01	G7K041	86.835	357	20	1	1	357	1	330	0.0	619

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TFs/TRs:

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Gene ID	Type	Classification
MsG0580025200.01.T01	TF	RWP-RK

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0580025200.01	MsG0780041048.01	0.809264	2.061547e-50	1.204824e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025200.01.T01	MTR_5g024070	86.835	357	20	1	1	357	1	330	0.0	619
MsG0580025200.01.T01	MTR_3g435550	36.296	135	78	2	208	341	38	165	1.88e-21	90.9
MsG0580025200.01.T01	MTR_3g091700	37.600	125	60	2	217	341	148	254	6.94e-19	85.5
MsG0580025200.01.T01	MTR_3g104830	48.276	87	40	2	226	310	393	476	2.59e-13	71.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025200.01.T01	AT4G35590	46.067	356	135	10	1	340	40	354	4.04e-86	265
MsG0580025200.01.T01	AT4G35590	46.199	342	127	10	15	340	4	304	1.52e-82	254
MsG0580025200.01.T01	AT1G18790	42.500	120	63	2	220	339	123	236	8.76e-22	94.0
MsG0580025200.01.T01	AT1G74480	45.217	115	58	2	226	338	131	242	1.02e-21	94.4
MsG0580025200.01.T01	AT5G66990	41.818	110	60	2	233	341	164	270	1.49e-19	87.8
MsG0580025200.01.T01	AT5G53040	41.739	115	54	1	227	341	147	248	2.23e-19	87.0
MsG0580025200.01.T01	AT5G16100	50.000	64	32	0	220	283	346	409	2.30e-13	71.2

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Find 62 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAAAACATAGAGAAG+AGG	0.578123	5:-15163611	MsG0580025200.01.T01:CDS
AAAAGGAAGTGCAGAGAATT+TGG	0.277802	5:-15164307	MsG0580025200.01.T01:CDS
AAAGATGTTGGAAAGTGAAA+AGG	0.459498	5:-15163628	MsG0580025200.01.T01:CDS
AAAGATTACTTTATCAGATT+TGG	0.267461	5:-15164395	MsG0580025200.01.T01:CDS
AAAGCAGGGAAACCTTGGTC+AGG	0.544103	5:+15165656	None:intergenic
AACATCTTGTCTGAATCTCT+TGG	0.478887	5:+15163563	None:intergenic
AAGAGGCAAAACATCAGGAA+AGG	0.561722	5:-15163689	MsG0580025200.01.T01:CDS
AAGGGTATAAGAATGGAGTT+TGG	0.381031	5:-15166251	MsG0580025200.01.T01:CDS
AATACAAATTGATTAAAGCA+GGG	0.588381	5:+15165642	None:intergenic
AATCTTTGCAACATGGTCAC+TGG	0.527182	5:+15164411	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGGAAAACATAGAGAAG+AGG	-	Chr5:15166499-15166518	MsG0580025200.01.T01:intron	30.0%
AAAGATGTTGGAAAGTGAAA+AGG	-	Chr5:15166482-15166501	MsG0580025200.01.T01:intron	30.0%
AAGTATGTTGTAGAACAGTT+AGG	+	Chr5:15164962-15164981	None:intergenic	30.0%
AGAGTTTGATAAAATCACAG+TGG	-	Chr5:15166178-15166197	MsG0580025200.01.T01:CDS	30.0%
AGTATGTTGTAGAACAGTTA+GGG	+	Chr5:15164961-15164980	None:intergenic	30.0%
ATACCTTGTAACCAACAAAA+AGG	+	Chr5:15163940-15163959	None:intergenic	30.0%
ATTAAAGAAGAACATGACAG+AGG	+	Chr5:15165151-15165170	None:intergenic	30.0%
ATTAGGAAGAAGTTATGCAT+TGG	+	Chr5:15165590-15165609	None:intergenic	30.0%
CGTCAAAGAATCTAAATGTT+GGG	-	Chr5:15165768-15165787	MsG0580025200.01.T01:intron	30.0%
CTCAGATTTCAAATCGTATT+TGG	+	Chr5:15164871-15164890	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	15163488	15166644	15163488	ID=MsG0580025200.01;Name=MsG0580025200.01
Chr5	mRNA	15163488	15166644	15163488	ID=MsG0580025200.01.T01;Parent=MsG0580025200.01;Name=MsG0580025200.01.T01;_AED=0.36;_eAED=0.43;_QI=0\|0\|0\|1\|0.8\|0.66\|6\|0\|357
Chr5	exon	15166593	15166644	15166593	ID=MsG0580025200.01.T01:exon:6195;Parent=MsG0580025200.01.T01
Chr5	exon	15166177	15166429	15166177	ID=MsG0580025200.01.T01:exon:6194;Parent=MsG0580025200.01.T01
Chr5	exon	15165614	15165694	15165614	ID=MsG0580025200.01.T01:exon:6193;Parent=MsG0580025200.01.T01
Chr5	exon	15164708	15164955	15164708	ID=MsG0580025200.01.T01:exon:6192;Parent=MsG0580025200.01.T01
Chr5	exon	15164246	15164460	15164246	ID=MsG0580025200.01.T01:exon:6191;Parent=MsG0580025200.01.T01
Chr5	exon	15163488	15163712	15163488	ID=MsG0580025200.01.T01:exon:6190;Parent=MsG0580025200.01.T01
Chr5	CDS	15166593	15166644	15166593	ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5	CDS	15166177	15166429	15166177	ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5	CDS	15165614	15165694	15165614	ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5	CDS	15164708	15164955	15164708	ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5	CDS	15164246	15164460	15164246	ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5	CDS	15163488	15163712	15163488	ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01

Gene Sequence

>MsG0580025200.01.T01

ATGGATTCCTCGCTCATTACTCTTCTTCTCTTCAACAACACTATTCAACCAGAGTTGATGAGAAGCGTGCATGTGTACCGTGAATGTGATGGAGGAGAAAAGGAAGTTGAAAGAGAGTTTGTGTTTTCTGAGAATGGTTCTTATGTTGAAATGAAAGCTACTCCAATTCTTAGATTGTTGAAATCTTTTGTTTCTGAAGAGATTTTTGAAGGGTATAAGAATGGAGTTTGGCTTTGTGTTTTTGCTTTTCATGCTTGTAATGTCCCTCAATTTTCTCACATTCCAACCTTTTTGTTGGTTACAAGGGCCTTAGCTTTTAGGCCTGACCAAGGTTTCCCTGCTTTAATCAATTTGTATTGTAAAACAGCACACAGAGCAGCAAAATGGAATCCAAAGCTTCAGGCCATTCCAAACCTGCTTAATGATCTCAACATGATATACAAGTTGGAACCGAAGAAGGAACGAAAAGATACACCAGAGAGCTCTGCAAGGGAAGAATTGGAAGGAAACAACAATGATTTCGAACCACTAAAGATAGTCTTGCCTGTGCTTAATCAGGATCTCAATTGTCTTCCTTTTGAAGAAGACGAGTCTGAGTTACTGGATGATGAGACAGATTTTGAAAGTTCAGCAGGTTTTTTAGGAAAGAAAAAGAGGGCAGCCAGTGACCATGTTGCAAAGATTACTTTATCAGATTTGGCCAAGTACTTTGATATGCCAATTGCTGAAGCGTCAAAGAATCTAAATGTTGGGCTTACTGTTCTCAAAAGGAAGTGCAGAGAATTTGGTATTCCTCGCTGGCCTCATAGGAAGATCAAATCACTCGACAGTCTCATTCATGATCTTCAGGAAGAGGCAAAACATCAGGAAAGGGAGGATAAAGATGCAGCCATGGCAGTGATAAGAAGGCAAAAGATGTTGGAAAGTGAAAAGGAAAACATAGAGAAGAGGCTCTTCATGGACATTCAGATTGAGACCAAGAGATTCAGACAAGATGTTTTCAAGAGAAGGCACAGAGCTAGAGTTATTGAAAACAGAATTCTACAGTTGCAAGCACCTAAACCTAGCAATTAG

Protein sequence

>MsG0580025200.01.T01

MDSSLITLLLFNNTIQPELMRSVHVYRECDGGEKEVEREFVFSENGSYVEMKATPILRLLKSFVSEEIFEGYKNGVWLCVFAFHACNVPQFSHIPTFLLVTRALAFRPDQGFPALINLYCKTAHRAAKWNPKLQAIPNLLNDLNMIYKLEPKKERKDTPESSAREELEGNNNDFEPLKIVLPVLNQDLNCLPFEEDESELLDDETDFESSAGFLGKKKRAASDHVAKITLSDLAKYFDMPIAEASKNLNVGLTVLKRKCREFGIPRWPHRKIKSLDSLIHDLQEEAKHQEREDKDAAMAVIRRQKMLESEKENIEKRLFMDIQIETKRFRQDVFKRRHRARVIENRILQLQAPKPSN*