AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025200.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025200.01.T01 MTR_5g024070 86.835 357 20 1 1 357 1 330 0.0 619
MsG0580025200.01.T01 MTR_3g435550 36.296 135 78 2 208 341 38 165 1.88e-21 90.9
MsG0580025200.01.T01 MTR_3g091700 37.600 125 60 2 217 341 148 254 6.94e-19 85.5
MsG0580025200.01.T01 MTR_3g104830 48.276 87 40 2 226 310 393 476 2.59e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025200.01.T01 AT4G35590 46.067 356 135 10 1 340 40 354 4.04e-86 265
MsG0580025200.01.T01 AT4G35590 46.199 342 127 10 15 340 4 304 1.52e-82 254
MsG0580025200.01.T01 AT1G18790 42.500 120 63 2 220 339 123 236 8.76e-22 94.0
MsG0580025200.01.T01 AT1G74480 45.217 115 58 2 226 338 131 242 1.02e-21 94.4
MsG0580025200.01.T01 AT5G66990 41.818 110 60 2 233 341 164 270 1.49e-19 87.8
MsG0580025200.01.T01 AT5G53040 41.739 115 54 1 227 341 147 248 2.23e-19 87.0
MsG0580025200.01.T01 AT5G16100 50.000 64 32 0 220 283 346 409 2.30e-13 71.2

Find 62 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATTACTTTATCAGATT+TGG 0.267461 5:-15164395 MsG0580025200.01.T01:CDS
AAAAGGAAGTGCAGAGAATT+TGG 0.277802 5:-15164307 MsG0580025200.01.T01:CDS
CAGTCTCATTCATGATCTTC+AGG 0.329225 5:-15164245 MsG0580025200.01.T01:intron
TTGAGATCATTAAGCAGGTT+TGG 0.348731 5:+15164911 None:intergenic
TTCTTTGACGCTTCAGCAAT+TGG 0.353848 5:+15164355 None:intergenic
TTTGGAATGGCCTGAAGCTT+TGG 0.353863 5:+15164929 None:intergenic
GATTTCAACAATCTAAGAAT+TGG 0.356192 5:+15166297 None:intergenic
AGAAGACGAGTCTGAGTTAC+TGG 0.356355 5:-15164738 MsG0580025200.01.T01:CDS
TTTCAGGAATCCAAAGCTTC+AGG 0.357774 5:-15164939 MsG0580025200.01.T01:intron
GAGCTCTGCAAGGGAAGAAT+TGG 0.370187 5:-15164840 MsG0580025200.01.T01:CDS
ATACCTTGTAACCAACAAAA+AGG 0.370413 5:+15166173 None:intergenic
GTATTCCTCGCTGGCCTCAT+AGG 0.379146 5:-15164285 MsG0580025200.01.T01:CDS
AAGGGTATAAGAATGGAGTT+TGG 0.381031 5:-15166251 MsG0580025200.01.T01:CDS
AGATGCTAATTGCTAGGTTT+AGG 0.384292 5:+15163483 None:intergenic
TCTTCCCTTGCAGAGCTCTC+TGG 0.396713 5:+15164845 None:intergenic
AGTCTTGCCTGTGCTTAATC+AGG 0.396954 5:-15164783 MsG0580025200.01.T01:CDS
CATAGAGAAGAGGCTCTTCA+TGG 0.402183 5:-15163601 MsG0580025200.01.T01:CDS
CAGCACACAGAGCAGCAAAA+TGG 0.403276 5:-15165613 MsG0580025200.01.T01:intron
CAATACAAATTGATTAAAGC+AGG 0.436782 5:+15165641 None:intergenic
TTTCTCCTCCATCACATTCA+CGG 0.441238 5:+15166382 None:intergenic
CGTCAAAGAATCTAAATGTT+GGG 0.449206 5:-15164342 MsG0580025200.01.T01:CDS
TTGCCTTCTTATCACTGCCA+TGG 0.457172 5:+15163650 None:intergenic
AAAGATGTTGGAAAGTGAAA+AGG 0.459498 5:-15163628 MsG0580025200.01.T01:CDS
TTGGGCTTACTGTTCTCAAA+AGG 0.466425 5:-15164324 MsG0580025200.01.T01:CDS
ACAGGCAAGACTATCTTTAG+TGG 0.472900 5:+15164794 None:intergenic
AACATCTTGTCTGAATCTCT+TGG 0.478887 5:+15163563 None:intergenic
TCAGACTCGTCTTCTTCAAA+AGG 0.484709 5:+15164746 None:intergenic
GAGAATTTGGTATTCCTCGC+TGG 0.487057 5:-15164294 MsG0580025200.01.T01:CDS
TCATGTTGAGATCATTAAGC+AGG 0.496279 5:+15164906 None:intergenic
CAGCCATGGCAGTGATAAGA+AGG 0.499451 5:-15163653 MsG0580025200.01.T01:CDS
GCAGGAAGAGGCAAAACATC+AGG 0.500010 5:-15163694 MsG0580025200.01.T01:intron
ATCATTAAGCAGGTTTGGAA+TGG 0.504221 5:+15164916 None:intergenic
GATAAGAAGGCAAAAGATGT+TGG 0.505266 5:-15163640 MsG0580025200.01.T01:CDS
TGATTAAAGCAGGGAAACCT+TGG 0.511760 5:+15165651 None:intergenic
TGAATGTGATGGAGGAGAAA+AGG 0.512445 5:-15166379 MsG0580025200.01.T01:CDS
TCTCAACATGATATACAAGT+TGG 0.522938 5:-15164894 MsG0580025200.01.T01:CDS
AATTGGCATATCAAAGTACT+TGG 0.526594 5:+15164372 None:intergenic
AATCTTTGCAACATGGTCAC+TGG 0.527182 5:+15164411 None:intergenic
TTGGAATGTGAGAAAATTGA+GGG 0.529395 5:+15166196 None:intergenic
ATACAAGTTGGAACCGAAGA+AGG 0.529415 5:-15164882 MsG0580025200.01.T01:CDS
AGAGGCAAAACATCAGGAAA+GGG 0.535237 5:-15163688 MsG0580025200.01.T01:CDS
ATCAGAAGATGCTAATTGCT+AGG 0.535738 5:+15163477 None:intergenic
AAAGCAGGGAAACCTTGGTC+AGG 0.544103 5:+15165656 None:intergenic
GCGTCAAAGAATCTAAATGT+TGG 0.549395 5:-15164343 MsG0580025200.01.T01:CDS
GTTGGAATGTGAGAAAATTG+AGG 0.552092 5:+15166195 None:intergenic
AAGAGGCAAAACATCAGGAA+AGG 0.561722 5:-15163689 MsG0580025200.01.T01:CDS
TTGGTCAGGCCTAAAAGCTA+AGG 0.570200 5:+15165670 None:intergenic
AAAAGGAAAACATAGAGAAG+AGG 0.578123 5:-15163611 MsG0580025200.01.T01:CDS
GAGAAGAAGAGTAATGAGCG+AGG 0.586084 5:+15166615 None:intergenic
AATACAAATTGATTAAAGCA+GGG 0.588381 5:+15165642 None:intergenic
GGAGGATAAAGATGCAGCCA+TGG 0.600209 5:-15163667 MsG0580025200.01.T01:CDS
AGTGATTTGATCTTCCTATG+AGG 0.604696 5:+15164271 None:intergenic
TTGAGATCCTGATTAAGCAC+AGG 0.605314 5:+15164776 None:intergenic
GGCAAAACATCAGGAAAGGG+AGG 0.624378 5:-15163685 MsG0580025200.01.T01:CDS
TACACCAGAGAGCTCTGCAA+GGG 0.628005 5:-15164849 MsG0580025200.01.T01:CDS
CATGTGTACCGTGAATGTGA+TGG 0.635852 5:-15166390 MsG0580025200.01.T01:CDS
ATACACCAGAGAGCTCTGCA+AGG 0.645197 5:-15164850 MsG0580025200.01.T01:CDS
ATAAAGTAATCTTTGCAACA+TGG 0.664575 5:+15164404 None:intergenic
TTCAACAACACTATTCAACC+AGG 0.665257 5:-15166592 MsG0580025200.01.T01:intron
ATCTTCCTATGAGGCCAGCG+AGG 0.669880 5:+15164280 None:intergenic
TCTGCAAGGGAAGAATTGGA+AGG 0.677605 5:-15164836 MsG0580025200.01.T01:CDS
GTGTACCGTGAATGTGATGG+AGG 0.689782 5:-15166387 MsG0580025200.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATACACTTATTTTATTAAT+AGG - Chr5:15165523-15165542 MsG0580025200.01.T01:intron 10.0%
!! AACAATTCAAATTCAATAAA+AGG + Chr5:15163977-15163996 None:intergenic 15.0%
!! ACAATTCAAATTCAATAAAA+GGG + Chr5:15163976-15163995 None:intergenic 15.0%
!! AGTCATAATAAATAAAAAAG+TGG + Chr5:15165898-15165917 None:intergenic 15.0%
!! TTATAAACCAAATACTTTAT+AGG + Chr5:15166308-15166327 None:intergenic 15.0%
!!! ATAACTATGTTTTTAAATAG+CGG - Chr5:15164054-15164073 MsG0580025200.01.T01:intron 15.0%
!!! TATTTTATTAATAGGAATCA+TGG - Chr5:15165531-15165550 MsG0580025200.01.T01:intron 15.0%
!! AAAGATTACTTTATCAGATT+TGG - Chr5:15165715-15165734 MsG0580025200.01.T01:intron 20.0%
!! AATACAAATTGATTAAAGCA+GGG + Chr5:15164471-15164490 None:intergenic 20.0%
!! ACATTTATATATCATTCACA+GGG + Chr5:15164545-15164564 None:intergenic 20.0%
!! ATGAAAATTTATAGTATCTG+AGG - Chr5:15166080-15166099 MsG0580025200.01.T01:intron 20.0%
!! CTAAATATTAGTTCAATAGT+TGG + Chr5:15165994-15166013 None:intergenic 20.0%
!! TAAAATAAGTGTATAAAGCT+AGG + Chr5:15165518-15165537 None:intergenic 20.0%
!! TACATTTATATATCATTCAC+AGG + Chr5:15164546-15164565 None:intergenic 20.0%
!! TATTTCAACCAAATACTATA+AGG + Chr5:15166055-15166074 None:intergenic 20.0%
!! TTGTAACTTAAACAAAATCA+CGG + Chr5:15166206-15166225 None:intergenic 20.0%
!!! GAATATGTTTTTTGATTTCT+TGG - Chr5:15165113-15165132 MsG0580025200.01.T01:intron 20.0%
!!! TATTGATTTCTTTATGTATG+TGG - Chr5:15164730-15164749 MsG0580025200.01.T01:CDS 20.0%
!!! TTTTTTAGGAAAGAAAAAGA+GGG - Chr5:15165673-15165692 MsG0580025200.01.T01:CDS 20.0%
!!! TTTTTTGTTGTTTTGTTTGT+GGG - Chr5:15163640-15163659 MsG0580025200.01.T01:CDS 20.0%
!!! TTTTTTTGTTGTTTTGTTTG+TGG - Chr5:15163639-15163658 MsG0580025200.01.T01:CDS 20.0%
! AGTTATCTAATCTAACAAAC+AGG + Chr5:15164688-15164707 None:intergenic 25.0%
! ATAAAGTAATCTTTGCAACA+TGG + Chr5:15165709-15165728 None:intergenic 25.0%
! ATTGCAAACCTTATAGTATT+TGG - Chr5:15166044-15166063 MsG0580025200.01.T01:intron 25.0%
! CAATACAAATTGATTAAAGC+AGG + Chr5:15164472-15164491 None:intergenic 25.0%
! GACATAATTAACTCAACATT+AGG + Chr5:15165607-15165626 None:intergenic 25.0%
! GATTTCAACAATCTAAGAAT+TGG + Chr5:15163816-15163835 None:intergenic 25.0%
! GTACAGAAAATATTTGTACA+AGG + Chr5:15166123-15166142 None:intergenic 25.0%
! GTTGTCAATTAGTATATGTT+TGG - Chr5:15166147-15166166 MsG0580025200.01.T01:intron 25.0%
!! GTAAATCATAGAAGTTTTCT+TGG + Chr5:15165969-15165988 None:intergenic 25.0%
!!! ATTTTTGAAGGGTATAAGAA+TGG - Chr5:15163852-15163871 MsG0580025200.01.T01:intron 25.0%
!!! CATGTTCTTCTTTAATTTTC+AGG - Chr5:15165155-15165174 MsG0580025200.01.T01:intron 25.0%
!!! GTTTTTTAGGAAAGAAAAAG+AGG - Chr5:15165672-15165691 MsG0580025200.01.T01:CDS 25.0%
!!! TTTCTGAAGAGATTTTTGAA+GGG - Chr5:15163841-15163860 MsG0580025200.01.T01:intron 25.0%
!!! TTTTTGTTGTTTTGTTTGTG+GGG - Chr5:15163641-15163660 MsG0580025200.01.T01:CDS 25.0%
AAAAGGAAAACATAGAGAAG+AGG - Chr5:15166499-15166518 MsG0580025200.01.T01:intron 30.0%
AAAGATGTTGGAAAGTGAAA+AGG - Chr5:15166482-15166501 MsG0580025200.01.T01:intron 30.0%
AAGTATGTTGTAGAACAGTT+AGG + Chr5:15164962-15164981 None:intergenic 30.0%
AGAGTTTGATAAAATCACAG+TGG - Chr5:15166178-15166197 MsG0580025200.01.T01:CDS 30.0%
AGTATGTTGTAGAACAGTTA+GGG + Chr5:15164961-15164980 None:intergenic 30.0%
ATACCTTGTAACCAACAAAA+AGG + Chr5:15163940-15163959 None:intergenic 30.0%
ATTAAAGAAGAACATGACAG+AGG + Chr5:15165151-15165170 None:intergenic 30.0%
ATTAGGAAGAAGTTATGCAT+TGG + Chr5:15165590-15165609 None:intergenic 30.0%
CGTCAAAGAATCTAAATGTT+GGG - Chr5:15165768-15165787 MsG0580025200.01.T01:intron 30.0%
CTCAGATTTCAAATCGTATT+TGG + Chr5:15164871-15164890 None:intergenic 30.0%
GGAATAAAATGTTCAAAAGC+AGG + Chr5:15165484-15165503 None:intergenic 30.0%
GTTCAAAAATCAGTTACCAA+TGG + Chr5:15164756-15164775 None:intergenic 30.0%
TAAGGAGTTCACTTAAAGAT+TGG + Chr5:15166377-15166396 None:intergenic 30.0%
TCTCAACATGATATACAAGT+TGG - Chr5:15165216-15165235 MsG0580025200.01.T01:intron 30.0%
TTATACAAAACAGTGTATCG+TGG + Chr5:15164136-15164155 None:intergenic 30.0%
TTATGTATTCTGCGTTTGTT+TGG - Chr5:15164013-15164032 MsG0580025200.01.T01:intron 30.0%
TTGGAATGTGAGAAAATTGA+GGG + Chr5:15163917-15163936 None:intergenic 30.0%
TTGGTGAAGTTAGAATTTGA+GGG - Chr5:15164982-15165001 MsG0580025200.01.T01:intron 30.0%
TTGTGAAATAGCAACTATAG+CGG - Chr5:15164160-15164179 MsG0580025200.01.T01:intron 30.0%
TTTGGTGAAGTTAGAATTTG+AGG - Chr5:15164981-15165000 MsG0580025200.01.T01:intron 30.0%
! AATTGGCATATCAAAGTACT+TGG + Chr5:15165741-15165760 None:intergenic 30.0%
! ACTGAAGCCTATAAAGTATT+TGG - Chr5:15166298-15166317 MsG0580025200.01.T01:CDS 30.0%
! ACTGTTCTACAACATACTTT+TGG - Chr5:15164963-15164982 MsG0580025200.01.T01:intron 30.0%
! TTTTTAAATAGCGGCTATAG+CGG - Chr5:15164063-15164082 MsG0580025200.01.T01:intron 30.0%
!! GTTGTTTTCATAAGCTATCT+TGG + Chr5:15164323-15164342 None:intergenic 30.0%
!! GTTTCTGAAGAGATTTTTGA+AGG - Chr5:15163840-15163859 MsG0580025200.01.T01:intron 30.0%
AACATCTTGTCTGAATCTCT+TGG + Chr5:15166550-15166569 None:intergenic 35.0%
ACTAAGTTGTTTATCCTGAC+AGG - Chr5:15164416-15164435 MsG0580025200.01.T01:CDS 35.0%
AGTGATTTGATCTTCCTATG+AGG + Chr5:15165842-15165861 None:intergenic 35.0%
ATCATTAAGCAGGTTTGGAA+TGG + Chr5:15165197-15165216 None:intergenic 35.0%
CTAAGTTGTTTATCCTGACA+GGG - Chr5:15164417-15164436 MsG0580025200.01.T01:CDS 35.0%
CTTGTAACCAACAAAAAGGT+TGG + Chr5:15163936-15163955 None:intergenic 35.0%
GAACAACGTGATAATTGACT+GGG + Chr5:15165462-15165481 None:intergenic 35.0%
GATAAGAAGGCAAAAGATGT+TGG - Chr5:15166470-15166489 MsG0580025200.01.T01:intron 35.0%
GATTCCTTACAGTTACGAAA+TGG - Chr5:15164779-15164798 MsG0580025200.01.T01:CDS 35.0%
GCGTCAAAGAATCTAAATGT+TGG - Chr5:15165767-15165786 MsG0580025200.01.T01:intron 35.0%
GTTGGAATGTGAGAAAATTG+AGG + Chr5:15163918-15163937 None:intergenic 35.0%
TAATGTCCCTACACAAGTAA+CGG - Chr5:15164593-15164612 MsG0580025200.01.T01:intron 35.0%
TATAGGCTTCAGTGCATATT+TGG + Chr5:15166291-15166310 None:intergenic 35.0%
TCATGTTGAGATCATTAAGC+AGG + Chr5:15165207-15165226 None:intergenic 35.0%
TTCAACAACACTATTCAACC+AGG - Chr5:15163518-15163537 MsG0580025200.01.T01:CDS 35.0%
TTCTTTATGTATGTGGCCAT+TGG - Chr5:15164737-15164756 MsG0580025200.01.T01:CDS 35.0%
TTGAGATCATTAAGCAGGTT+TGG + Chr5:15165202-15165221 None:intergenic 35.0%
TTGATAAAGTGAGTGTTTGC+AGG - Chr5:15165650-15165669 MsG0580025200.01.T01:CDS 35.0%
TTGTAGAACAGTTAGGGAAA+TGG + Chr5:15164955-15164974 None:intergenic 35.0%
TTTCGTAACTGTAAGGAATC+CGG + Chr5:15164779-15164798 None:intergenic 35.0%
! AAAAGGAAGTGCAGAGAATT+TGG - Chr5:15165803-15165822 MsG0580025200.01.T01:intron 35.0%
! AAGGGTATAAGAATGGAGTT+TGG - Chr5:15163859-15163878 MsG0580025200.01.T01:intron 35.0%
! CATAGCGGAATTTGAACAAA+CGG - Chr5:15164102-15164121 MsG0580025200.01.T01:intron 35.0%
! GTATCTTTTCGTTCCTTCTT+CGG + Chr5:15165244-15165263 None:intergenic 35.0%
! GTTATAGTGCTATTGCATAG+CGG - Chr5:15164087-15164106 MsG0580025200.01.T01:intron 35.0%
! TAGCTTTTGTGCCAAAATCA+CGG - Chr5:15166230-15166249 MsG0580025200.01.T01:CDS 35.0%
! TATCTGAGGCTTCTTTAGAT+AGG - Chr5:15166094-15166113 MsG0580025200.01.T01:intron 35.0%
! TCACATTCCAACCTTTTTGT+TGG - Chr5:15163926-15163945 MsG0580025200.01.T01:intron 35.0%
!! ACAGATTTTGAAAGTTCAGC+AGG - Chr5:15165403-15165422 MsG0580025200.01.T01:intron 35.0%
!! CAACCTTTTTGTTGGTTACA+AGG - Chr5:15163934-15163953 MsG0580025200.01.T01:intron 35.0%
!! GACAAGATGTTTTCAAGAGA+AGG - Chr5:15166559-15166578 MsG0580025200.01.T01:intron 35.0%
!! GAGTTTGTGTTTTCTGAGAA+TGG - Chr5:15163765-15163784 MsG0580025200.01.T01:intron 35.0%
!! TGGTAACTGATTTTTGAACC+CGG - Chr5:15164757-15164776 MsG0580025200.01.T01:CDS 35.0%
!!! GTGGGGTGTTTTTTGTTTTT+GGG - Chr5:15163658-15163677 MsG0580025200.01.T01:CDS 35.0%
!!! TGAGTGTTTGCAGGTTTTTT+AGG - Chr5:15165659-15165678 MsG0580025200.01.T01:CDS 35.0%
!!! TGGGGTGTTTTTTGTTTTTG+GGG - Chr5:15163659-15163678 MsG0580025200.01.T01:CDS 35.0%
!!! TGTGGGGTGTTTTTTGTTTT+TGG - Chr5:15163657-15163676 MsG0580025200.01.T01:CDS 35.0%
AAGAGGCAAAACATCAGGAA+AGG - Chr5:15166421-15166440 MsG0580025200.01.T01:intron 40.0%
AATCTTTGCAACATGGTCAC+TGG + Chr5:15165702-15165721 None:intergenic 40.0%
ACAGGCAAGACTATCTTTAG+TGG + Chr5:15165319-15165338 None:intergenic 40.0%
AGAACATGACAGAGGAAGTT+AGG + Chr5:15165143-15165162 None:intergenic 40.0%
AGAGGCAAAACATCAGGAAA+GGG - Chr5:15166422-15166441 MsG0580025200.01.T01:intron 40.0%
AGCAGGTTTGAAAGTTTACC+AGG - Chr5:15165420-15165439 MsG0580025200.01.T01:intron 40.0%
CAGTCTCATTCATGATCTTC+AGG - Chr5:15165865-15165884 MsG0580025200.01.T01:intron 40.0%
GAAATGGTGCAGTAGATGTT+TGG - Chr5:15164795-15164814 MsG0580025200.01.T01:CDS 40.0%
GAAGTTAGAATTTGAGGGCA+TGG - Chr5:15164987-15165006 MsG0580025200.01.T01:intron 40.0%
GCGATGTTGTCATGTGTATA+AGG + Chr5:15166395-15166414 None:intergenic 40.0%
GCTTCAGTGCATATTTGGAT+TGG + Chr5:15166286-15166305 None:intergenic 40.0%
GGAACAACGTGATAATTGAC+TGG + Chr5:15165463-15165482 None:intergenic 40.0%
GGTGTGTACTGTGTACAAAT+TGG - Chr5:15164518-15164537 MsG0580025200.01.T01:intron 40.0%
TAAAGCTAGGACTGAGTTGT+TGG + Chr5:15165505-15165524 None:intergenic 40.0%
TCAGACTCGTCTTCTTCAAA+AGG + Chr5:15165367-15165386 None:intergenic 40.0%
TCATCCTACAGTTGCAATTG+CGG - Chr5:15164902-15164921 MsG0580025200.01.T01:CDS 40.0%
TGATTAAAGCAGGGAAACCT+TGG + Chr5:15164462-15164481 None:intergenic 40.0%
TGCACCATTTCGTAACTGTA+AGG + Chr5:15164786-15164805 None:intergenic 40.0%
TTCGTAACTGTAAGGAATCC+GGG + Chr5:15164778-15164797 None:intergenic 40.0%
TTCTTTGACGCTTCAGCAAT+TGG + Chr5:15165758-15165777 None:intergenic 40.0%
TTGAGATCCTGATTAAGCAC+AGG + Chr5:15165337-15165356 None:intergenic 40.0%
TTGGGCTTACTGTTCTCAAA+AGG - Chr5:15165786-15165805 MsG0580025200.01.T01:intron 40.0%
TTTCAGGAATCCAAAGCTTC+AGG - Chr5:15165171-15165190 MsG0580025200.01.T01:intron 40.0%
TTTCTCCTCCATCACATTCA+CGG + Chr5:15163731-15163750 None:intergenic 40.0%
! ATACAAGTTGGAACCGAAGA+AGG - Chr5:15165228-15165247 MsG0580025200.01.T01:intron 40.0%
! GGAGCATTTGAATCTAAACC+TGG + Chr5:15165441-15165460 None:intergenic 40.0%
! TGAATGTGATGGAGGAGAAA+AGG - Chr5:15163731-15163750 MsG0580025200.01.T01:intron 40.0%
!!! GGGGTGTTTTTTGTTTTTGG+GGG - Chr5:15163660-15163679 MsG0580025200.01.T01:CDS 40.0%
!!! GGGTGTTTTTTGTTTTTGGG+GGG - Chr5:15163661-15163680 MsG0580025200.01.T01:CDS 40.0%
AGAAGACGAGTCTGAGTTAC+TGG - Chr5:15165372-15165391 MsG0580025200.01.T01:intron 45.0%
AGTCTTGCCTGTGCTTAATC+AGG - Chr5:15165327-15165346 MsG0580025200.01.T01:intron 45.0%
CATAGAGAAGAGGCTCTTCA+TGG - Chr5:15166509-15166528 MsG0580025200.01.T01:intron 45.0%
CATGTGTACCGTGAATGTGA+TGG - Chr5:15163720-15163739 MsG0580025200.01.T01:intron 45.0%
GAGAAGAAGAGTAATGAGCG+AGG + Chr5:15163498-15163517 None:intergenic 45.0%
GAGAATTTGGTATTCCTCGC+TGG - Chr5:15165816-15165835 MsG0580025200.01.T01:intron 45.0%
GATTCGCCGTTACTTGTGTA+GGG + Chr5:15164602-15164621 None:intergenic 45.0%
GGTATTGCATTGCATGTACC+TGG + Chr5:15163539-15163558 None:intergenic 45.0%
GTGCAGTAGATGTTTGGCAT+TGG - Chr5:15164801-15164820 MsG0580025200.01.T01:CDS 45.0%
TCTGCAAGGGAAGAATTGGA+AGG - Chr5:15165274-15165293 MsG0580025200.01.T01:intron 45.0%
TGATTCGCCGTTACTTGTGT+AGG + Chr5:15164603-15164622 None:intergenic 45.0%
TTGCCTTCTTATCACTGCCA+TGG + Chr5:15166463-15166482 None:intergenic 45.0%
TTGGTCAGGCCTAAAAGCTA+AGG + Chr5:15164443-15164462 None:intergenic 45.0%
! TGCTGCAAATGTTGACTGCA+GGG - Chr5:15164926-15164945 MsG0580025200.01.T01:CDS 45.0%
! TTGAGGGCATGGTTCAACAA+TGG - Chr5:15164998-15165017 MsG0580025200.01.T01:intron 45.0%
! TTTGGAATGGCCTGAAGCTT+TGG + Chr5:15165184-15165203 None:intergenic 45.0%
!! TTAGCTTTTAGGCCTGACCA+AGG - Chr5:15164442-15164461 MsG0580025200.01.T01:intron 45.0%
!! AAAATTGAAAAAATAAAAAA+TGG + Chr5:15163561-15163580 None:intergenic 5.0%
!! AAATTGAAAAAATAAAAAAT+GGG + Chr5:15163560-15163579 None:intergenic 5.0%
!!! AAATTTTGATTTTTTTTTAA+TGG - Chr5:15163597-15163616 MsG0580025200.01.T01:CDS 5.0%
!!! AATTTTGATTTTTTTTTAAT+GGG - Chr5:15163598-15163617 MsG0580025200.01.T01:CDS 5.0%
AAAGCAGGGAAACCTTGGTC+AGG + Chr5:15164457-15164476 None:intergenic 50.0%
ACACATGACAACATCGCTGC+AGG - Chr5:15166398-15166417 MsG0580025200.01.T01:CDS 50.0%
ATACACCAGAGAGCTCTGCA+AGG - Chr5:15165260-15165279 MsG0580025200.01.T01:intron 50.0%
CAGCACACAGAGCAGCAAAA+TGG - Chr5:15164497-15164516 MsG0580025200.01.T01:intron 50.0%
CAGCCATGGCAGTGATAAGA+AGG - Chr5:15166457-15166476 MsG0580025200.01.T01:intron 50.0%
CTAAAAGCTAAGGCCCTGTC+AGG + Chr5:15164433-15164452 None:intergenic 50.0%
GAGCTCTGCAAGGGAAGAAT+TGG - Chr5:15165270-15165289 MsG0580025200.01.T01:intron 50.0%
GCAGGAAGAGGCAAAACATC+AGG - Chr5:15166416-15166435 MsG0580025200.01.T01:intron 50.0%
GGAGGATAAAGATGCAGCCA+TGG - Chr5:15166443-15166462 MsG0580025200.01.T01:intron 50.0%
GGCAAAACATCAGGAAAGGG+AGG - Chr5:15166425-15166444 MsG0580025200.01.T01:intron 50.0%
GGGCATGGTTCAACAATGGA+TGG - Chr5:15165002-15165021 MsG0580025200.01.T01:intron 50.0%
GTGTACCGTGAATGTGATGG+AGG - Chr5:15163723-15163742 MsG0580025200.01.T01:intron 50.0%
TACACCAGAGAGCTCTGCAA+GGG - Chr5:15165261-15165280 MsG0580025200.01.T01:intron 50.0%
TAGCAACTATAGCGGCGCTA+TGG - Chr5:15164168-15164187 MsG0580025200.01.T01:intron 50.0%
! ACGGAGAGCAACCGTGATTT+TGG + Chr5:15166244-15166263 None:intergenic 50.0%
! CTGCTGCAAATGTTGACTGC+AGG - Chr5:15164925-15164944 MsG0580025200.01.T01:CDS 50.0%
!! CTGACAGGGCCTTAGCTTTT+AGG - Chr5:15164431-15164450 MsG0580025200.01.T01:CDS 50.0%
ACGGTTGCTCTCCGTGATTC+GGG - Chr5:15166249-15166268 MsG0580025200.01.T01:CDS 55.0%
ATCTTCCTATGAGGCCAGCG+AGG + Chr5:15165833-15165852 None:intergenic 55.0%
CACGGTTGCTCTCCGTGATT+CGG - Chr5:15166248-15166267 MsG0580025200.01.T01:CDS 55.0%
CGTTGAGACTGCCCGAATCA+CGG + Chr5:15166263-15166282 None:intergenic 55.0%
GACAACATCGCTGCAGGAAG+AGG - Chr5:15166404-15166423 MsG0580025200.01.T01:CDS 55.0%
GCAGCCGCAATTGCAACTGT+AGG + Chr5:15164909-15164928 None:intergenic 55.0%
GCTATGGCAGTGTTGCATGG+CGG - Chr5:15164184-15164203 MsG0580025200.01.T01:intron 55.0%
TCTTCCCTTGCAGAGCTCTC+TGG + Chr5:15165268-15165287 None:intergenic 55.0%
! GTATTCCTCGCTGGCCTCAT+AGG - Chr5:15165825-15165844 MsG0580025200.01.T01:intron 55.0%
GGCGCTATGGCAGTGTTGCA+TGG - Chr5:15164181-15164200 MsG0580025200.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 15163488 15166644 15163488 ID=MsG0580025200.01;Name=MsG0580025200.01
Chr5 mRNA 15163488 15166644 15163488 ID=MsG0580025200.01.T01;Parent=MsG0580025200.01;Name=MsG0580025200.01.T01;_AED=0.36;_eAED=0.43;_QI=0|0|0|1|0.8|0.66|6|0|357
Chr5 exon 15166593 15166644 15166593 ID=MsG0580025200.01.T01:exon:6195;Parent=MsG0580025200.01.T01
Chr5 exon 15166177 15166429 15166177 ID=MsG0580025200.01.T01:exon:6194;Parent=MsG0580025200.01.T01
Chr5 exon 15165614 15165694 15165614 ID=MsG0580025200.01.T01:exon:6193;Parent=MsG0580025200.01.T01
Chr5 exon 15164708 15164955 15164708 ID=MsG0580025200.01.T01:exon:6192;Parent=MsG0580025200.01.T01
Chr5 exon 15164246 15164460 15164246 ID=MsG0580025200.01.T01:exon:6191;Parent=MsG0580025200.01.T01
Chr5 exon 15163488 15163712 15163488 ID=MsG0580025200.01.T01:exon:6190;Parent=MsG0580025200.01.T01
Chr5 CDS 15166593 15166644 15166593 ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5 CDS 15166177 15166429 15166177 ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5 CDS 15165614 15165694 15165614 ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5 CDS 15164708 15164955 15164708 ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5 CDS 15164246 15164460 15164246 ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Chr5 CDS 15163488 15163712 15163488 ID=MsG0580025200.01.T01:cds;Parent=MsG0580025200.01.T01
Gene Sequence

>MsG0580025200.01.T01

ATGGATTCCTCGCTCATTACTCTTCTTCTCTTCAACAACACTATTCAACCAGAGTTGATGAGAAGCGTGCATGTGTACCGTGAATGTGATGGAGGAGAAAAGGAAGTTGAAAGAGAGTTTGTGTTTTCTGAGAATGGTTCTTATGTTGAAATGAAAGCTACTCCAATTCTTAGATTGTTGAAATCTTTTGTTTCTGAAGAGATTTTTGAAGGGTATAAGAATGGAGTTTGGCTTTGTGTTTTTGCTTTTCATGCTTGTAATGTCCCTCAATTTTCTCACATTCCAACCTTTTTGTTGGTTACAAGGGCCTTAGCTTTTAGGCCTGACCAAGGTTTCCCTGCTTTAATCAATTTGTATTGTAAAACAGCACACAGAGCAGCAAAATGGAATCCAAAGCTTCAGGCCATTCCAAACCTGCTTAATGATCTCAACATGATATACAAGTTGGAACCGAAGAAGGAACGAAAAGATACACCAGAGAGCTCTGCAAGGGAAGAATTGGAAGGAAACAACAATGATTTCGAACCACTAAAGATAGTCTTGCCTGTGCTTAATCAGGATCTCAATTGTCTTCCTTTTGAAGAAGACGAGTCTGAGTTACTGGATGATGAGACAGATTTTGAAAGTTCAGCAGGTTTTTTAGGAAAGAAAAAGAGGGCAGCCAGTGACCATGTTGCAAAGATTACTTTATCAGATTTGGCCAAGTACTTTGATATGCCAATTGCTGAAGCGTCAAAGAATCTAAATGTTGGGCTTACTGTTCTCAAAAGGAAGTGCAGAGAATTTGGTATTCCTCGCTGGCCTCATAGGAAGATCAAATCACTCGACAGTCTCATTCATGATCTTCAGGAAGAGGCAAAACATCAGGAAAGGGAGGATAAAGATGCAGCCATGGCAGTGATAAGAAGGCAAAAGATGTTGGAAAGTGAAAAGGAAAACATAGAGAAGAGGCTCTTCATGGACATTCAGATTGAGACCAAGAGATTCAGACAAGATGTTTTCAAGAGAAGGCACAGAGCTAGAGTTATTGAAAACAGAATTCTACAGTTGCAAGCACCTAAACCTAGCAATTAG
Protein sequence

>MsG0580025200.01.T01

MDSSLITLLLFNNTIQPELMRSVHVYRECDGGEKEVEREFVFSENGSYVEMKATPILRLLKSFVSEEIFEGYKNGVWLCVFAFHACNVPQFSHIPTFLLVTRALAFRPDQGFPALINLYCKTAHRAAKWNPKLQAIPNLLNDLNMIYKLEPKKERKDTPESSAREELEGNNNDFEPLKIVLPVLNQDLNCLPFEEDESELLDDETDFESSAGFLGKKKRAASDHVAKITLSDLAKYFDMPIAEASKNLNVGLTVLKRKCREFGIPRWPHRKIKSLDSLIHDLQEEAKHQEREDKDAAMAVIRRQKMLESEKENIEKRLFMDIQIETKRFRQDVFKRRHRARVIENRILQLQAPKPSN*