AlfalfaGEDB | MsG0580025299.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025299.01

KEGG: -
GO: GO:0004672 GO:0005515 GO:0005524 GO:0006468
PFAM: PF00069 PF00560 PF08263

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025299.01.T01	XP_003612525.1	75.932	993	226	7	1	983	22	1011	0	1486

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025299.01.T01	C0LGP4	43.763	994	525	12	7	980	22	1001	0	801

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025299.01.T01	G7K5D3	75.932	993	226	7	1	983	22	1011	0.0	1486

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180001578.01	MsG0580025299.01	PPI
MsG0180001578.01	MsG0580025299.01	PPI
MsG0180001580.01	MsG0580025299.01	PPI
MsG0180001581.01	MsG0580025299.01	PPI
MsG0180001654.01	MsG0580025299.01	PPI
MsG0380015449.01	MsG0580025299.01	PPI
MsG0380015449.01	MsG0580025299.01	PPI
MsG0580025299.01	MsG0180001594.01	PPI
MsG0580025299.01	MsG0180001650.01	PPI
MsG0580025299.01	MsG0180001581.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0580025299.01.T01	MTR_5g026000	75.932	993	226	7	1	983	22	1011	1486
MsG0580025299.01.T01	MTR_5g019070	76.583	995	221	6	2	986	23	1015	1475
MsG0580025299.01.T01	MTR_5g026010	75.276	995	235	5	1	986	22	1014	1458
MsG0580025299.01.T01	MTR_5g044680	66.229	989	323	5	4	983	37	1023	1247
MsG0580025299.01.T01	MTR_5g026150	65.660	993	329	6	2	984	4	994	1243
MsG0580025299.01.T01	MTR_5g026160	64.343	990	335	7	2	982	11	991	1237
MsG0580025299.01.T01	MTR_2g040910	64.729	998	334	7	2	986	35	1027	1234
MsG0580025299.01.T01	MTR_5g024450	65.319	989	332	5	4	983	43	1029	1233
MsG0580025299.01.T01	MTR_5g025950	64.914	989	336	5	4	983	38	1024	1228
MsG0580025299.01.T01	MTR_6g036840	63.928	998	342	7	2	986	35	1027	1227

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025299.01.T01	AT3G47570	43.763	994	525	12	7	980	22	1001	0.0	801
MsG0580025299.01.T01	AT5G20480	42.388	1005	532	11	3	984	24	1004	0.0	763
MsG0580025299.01.T01	AT5G20480	42.388	1005	532	11	3	984	24	1004	0.0	763
MsG0580025299.01.T01	AT3G47090	42.528	997	540	13	7	984	22	1004	0.0	748
MsG0580025299.01.T01	AT3G47580	42.042	999	544	11	7	984	22	1006	0.0	747
MsG0580025299.01.T01	AT3G47110	43.682	1005	525	14	2	981	32	1020	0.0	738
MsG0580025299.01.T01	AT2G24130	37.486	915	490	22	127	982	71	962	1.58e-164	508
MsG0580025299.01.T01	AT2G24130	34.717	265	169	4	52	312	299	563	1.66e-24	111
MsG0580025299.01.T01	AT2G24130	27.064	436	227	11	41	441	185	564	1.68e-24	111
MsG0580025299.01.T01	AT2G24130	33.597	253	152	7	342	579	65	316	1.35e-23	108

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Find 190 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAGATTGCCAACAT+GGG	0.557558	5:+16658924	None:intergenic
AAAATATGTATAAGATTATT+AGG	0.330447	5:+16656291	None:intergenic
AAACGGAGTGAAATTGCCAC+TGG	0.623161	5:+16656215	None:intergenic
AAACTGGAAGCTTAGAACAA+TGG	0.635382	5:-16656845	MsG0580025299.01.T01:CDS
AAACTTAGTCAACTAGATAT+TGG	0.282952	5:-16658097	MsG0580025299.01.T01:CDS
AAAGAGAGATTGCCAACATG+GGG	0.658204	5:+16658925	None:intergenic
AAATATGAGACATCGAAATT+TGG	0.225167	5:-16656943	MsG0580025299.01.T01:CDS
AAATCCCACAGGAACTTGGT+CGG	0.648889	5:-16658861	MsG0580025299.01.T01:CDS
AACAACCGACCAAGTTCCTG+TGG	0.617878	5:+16658856	None:intergenic
AACGTATAATAGTTGAAGAT+GGG	0.548172	5:+16658405	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCTTGTAGAGTATGGAAT+TGG	-	Chr5:16658753-16658772	MsG0580025299.01.T01:CDS	30.0%
AATGCAACTATTAGGATTGA+TGG	-	Chr5:16657249-16657268	MsG0580025299.01.T01:CDS	30.0%
AGCCAATGTAAGAATAGTTA+GGG	+	Chr5:16656588-16656607	None:intergenic	30.0%
ATCCCTAACTATTCTTACAT+TGG	-	Chr5:16656583-16656602	MsG0580025299.01.T01:CDS	30.0%
ATCTAGTTGACTAAGTTTAG+TGG	+	Chr5:16657101-16657120	None:intergenic	30.0%
ATGCAACTATTAGGATTGAT+GGG	-	Chr5:16657250-16657269	MsG0580025299.01.T01:CDS	30.0%
ATTAGATTTCCTAATTCTGC+AGG	+	Chr5:16657154-16657173	None:intergenic	30.0%
CAAAGAAACTATTGTTTGCA+AGG	+	Chr5:16656316-16656335	None:intergenic	30.0%
CAACGTATAATAGTTGAAGA+TGG	+	Chr5:16656799-16656818	None:intergenic	30.0%
CAGATTTATTATCTATGGCA+TGG	+	Chr5:16657900-16657919	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	16656074	16659148	16656074	ID=MsG0580025299.01;Name=MsG0580025299.01
Chr5	mRNA	16656074	16659148	16656074	ID=MsG0580025299.01.T01;Parent=MsG0580025299.01;Name=MsG0580025299.01.T01;_AED=0.35;_eAED=0.35;_QI=0\|0\|0\|1\|1\|1\|2\|0\|986
Chr5	exon	16656574	16659148	16656574	ID=MsG0580025299.01.T01:exon:30250;Parent=MsG0580025299.01.T01
Chr5	exon	16656074	16656459	16656074	ID=MsG0580025299.01.T01:exon:30249;Parent=MsG0580025299.01.T01
Chr5	CDS	16656574	16659148	16656574	ID=MsG0580025299.01.T01:cds;Parent=MsG0580025299.01.T01
Chr5	CDS	16656074	16656459	16656074	ID=MsG0580025299.01.T01:cds;Parent=MsG0580025299.01.T01

Gene Sequence

>MsG0580025299.01.T01

ATGACCTCTACCTTAGGAAATGAGACCGATAACTTGGCGTTGCTCAAATTCAAAGAATCAATATCTAATGATTATCTGTATGGAATCTTAGCCTCTTGGAATAGGTCAACTCACTACTGTAATTGGCATGGAATTACATGTAGTCAACAAAGAGTTACAAAGTTAGACTTACAAGGGTATAAGTTTCATGGATTCATATCTCCCCATGTTGGCAATCTCTCTTTTTTGACAACTCTAAACCTTGCAAACAATAGTTTCTTTGGAAAAATCCCACAGGAACTTGGTCGGTTGTTTCGATTGCAACAACTCATTCTCACCAATAACTCCTTTACAGGGGAGATTCCAACAAACTTGTCAAGTTGCTCTGATCTTGAATACTTATTCTTATCTGGAAATCATCTGATTGGTAAAATACCAATTGGAATCAACTTTCTTCATAAGCTTCTGTTGTTTGACATTACAAATAACAATTTAACTGGAAGAATTTCACCATTCATAGGGAATCTTTCATCCCTAACTATTCTTACATTGGCTATGAATAACTTAGAAGGAGATATACCACAAGAAATCTGTAGCCTCAAAAATTTGAAAAAAATTAGAGTGGCTATAAACAGACTATCAGGTACATTTCATTCTTGTCTTTATAATATGTCATCTCTTACTATGATCGCAGCTGCAATGAATGAGTTTAATGGATCTCTTCCATTCAATATGTTCAACACCCATCTTCAACTATTATACGTTGGAGGAAATAAATTCTCAGGTGCAATACCTATTTCCATTGCAAATGCTTCTGCCTTACAACAACTCGACTTAAGTGGAAATAAATTGTCCGGACAAGTTCCAAATCTAGGGAAGTTACATGATCTTCAATATCTAAATCTAGAACTAAACAATTTAGGTGACAATTCAACTAAGGATTTAGAGTTCTTAAAATCCATGACAAATTGTAGTAACCTACAAGTGATTTCTATATATGATAATAATTTTGGCGGAAATTTGCCAAACTCTGTAGGCAATTTATCCACTAAACTTAGTCAACTAGATATTGGGCTCAATAAGATATCAGGAAATGTTCCTGCAGAATTAGGAAATCTAATTGGCTTAATTCTATTGAGCCTGAATTTTAACCACTTCGTAGGAATTATTCCTGATACTTTTGGGAAGCTTGAGAAAATGCAACTATTAGGATTGATGGGAAATAAGTTGTTCGGAGAGATACCACCCTTCATAGGAAACCTTAGTCAATTATATCGTTTGCAAATAGGACTTAATCTGTTCGAAGGAAATGTTCCTTCAAGTATAGGAAATTGCACAAAGTTACAACACCTAGATCTTTCACAAAACAATCTTAGAGGAACTATACCATTAGAGGTTTTCAGGCTTTCTTCGTTATCAATCTTATTGGACTTGTCGAGAAACTCATTGAGTGGCAGCTTGCCGAGAGAAGTGGGTAAGTTAATAAGCATCAACAAGATAGATTTCTCGGAGAATCATCTATCTGGTGAAATTCCTAGAACGATTGGTGAATGCATAGGCTTAGAATACCTCTTTTTCCAAGGGAACTCATTCAATGGAGCCATACCATCCTCGTTGGATTCACTAAAAGGTCTTCAATATTTAGACTTGTCAAGAAATCGCCTATCTGGACCAATTCCTAATGTTCTTCAAAATATATCAGTTTTAGAACACTTGAATGTTTCTTTCAAAATGTTGGAAGGAGAGGTACCAAAAGAAGGTGTCTTTGGAAATGTAAGCAGATTCATTGTGACTGGAAACAATAAGCTTTGTGGAGGAATTTCAAAGCTTCATCTACAACCATGCCATGCCATAGATAATAAATCTGCAAAACACCATACCCTCAAGTTGATAGTAGTGATAGTTAGCGTGGCATCTATTCTTATTATGGTGACAGTTATTCTAACTATCTACCAGTTGATGAAAAGAAACAAGAAACAATATTCCGATCCACCAATAACTGACCCGCTTGCCAGGGTTTCATACAAAGACCTTCACAAAGGAACTGATGGATTTTCTGATAGAAACTTGGTTGGATCTGGAAATTTTGGCTGTGTGTACAAAGGATATATGTTCTCAGAATGTAAATATGTTGCCATAAAGGTCCTAAACCTTCAGCAGAAGGGAGCTCACAAAAGTTTCATTACTGAATGTAATGCACTGAAAAATATGAGACATCGAAATTTGGTTAAAATTTTGACATGTTGTTCAAGTACAGATTACAATGGTCAAGAATTCAAGGCTTTAGTGTTTGAATACATGAAAACTGGAAGCTTAGAACAATGGCTATATCCAGGGAATCAAAGAAAGTTGGACCTTGATCAAAGATTGAATATCATTGTGGACATTGCTTCTGTATTACATTACCTTCATCATGAATGTGAGCAATCGGTCATTCATTGTGATCTGAAGCCAAGCAATGTCCTTCTTGATGACGACATGGTTGCTCATGTTAGTGATTTTGGCATAGCAAGACTTGTCTCAGTTATTGAAGACTCATCTCATCAGGAAACTAGTACAATTGGAATTAAAGGGACTGTTGGCTATGCCCCTCCAGAGTATGGAATTGGTTCTGAAGTATCCACATATGGTGACATGTATAGCTTTGGGATGCTTTTGTTGGAAATTCTTACAGGAAGGAGACCAGTTGATGAAATGTTTGATGATTCTCAAAATCTGCGTATGTTTGTTGAAACTTCATTGCCTAATAATCTTATACATATTTTGGACCGATATCTTGTACCAAGAAATCTAGAACCAACAATAGAAGATGACATCACCAGTGGCAATTTCACTCCGTTTGTAGAGAAGTGCTTAGTTTCACTTTTTAGAATTGGACTTGCCTGTTCAGTGGAGACACCAAAAGAAAGAATGGATATAATTGATGTCACCAGAGAATTAAGCATAATCAAAAAGGCTTATCTCGCTGGTAAGTTTGATTGA

Protein sequence

>MsG0580025299.01.T01

MTSTLGNETDNLALLKFKESISNDYLYGILASWNRSTHYCNWHGITCSQQRVTKLDLQGYKFHGFISPHVGNLSFLTTLNLANNSFFGKIPQELGRLFRLQQLILTNNSFTGEIPTNLSSCSDLEYLFLSGNHLIGKIPIGINFLHKLLLFDITNNNLTGRISPFIGNLSSLTILTLAMNNLEGDIPQEICSLKNLKKIRVAINRLSGTFHSCLYNMSSLTMIAAAMNEFNGSLPFNMFNTHLQLLYVGGNKFSGAIPISIANASALQQLDLSGNKLSGQVPNLGKLHDLQYLNLELNNLGDNSTKDLEFLKSMTNCSNLQVISIYDNNFGGNLPNSVGNLSTKLSQLDIGLNKISGNVPAELGNLIGLILLSLNFNHFVGIIPDTFGKLEKMQLLGLMGNKLFGEIPPFIGNLSQLYRLQIGLNLFEGNVPSSIGNCTKLQHLDLSQNNLRGTIPLEVFRLSSLSILLDLSRNSLSGSLPREVGKLISINKIDFSENHLSGEIPRTIGECIGLEYLFFQGNSFNGAIPSSLDSLKGLQYLDLSRNRLSGPIPNVLQNISVLEHLNVSFKMLEGEVPKEGVFGNVSRFIVTGNNKLCGGISKLHLQPCHAIDNKSAKHHTLKLIVVIVSVASILIMVTVILTIYQLMKRNKKQYSDPPITDPLARVSYKDLHKGTDGFSDRNLVGSGNFGCVYKGYMFSECKYVAIKVLNLQQKGAHKSFITECNALKNMRHRNLVKILTCCSSTDYNGQEFKALVFEYMKTGSLEQWLYPGNQRKLDLDQRLNIIVDIASVLHYLHHECEQSVIHCDLKPSNVLLDDDMVAHVSDFGIARLVSVIEDSSHQETSTIGIKGTVGYAPPEYGIGSEVSTYGDMYSFGMLLLEILTGRRPVDEMFDDSQNLRMFVETSLPNNLIHILDRYLVPRNLEPTIEDDITSGNFTPFVEKCLVSLFRIGLACSVETPKERMDIIDVTRELSIIKKAYLAGKFD*