AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025512.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025512.01.T01 MTR_5g030010 93.634 377 18 3 1 376 1 372 0.0 672
MsG0580025512.01.T01 MTR_8g066780 55.733 375 136 10 7 376 7 356 2.61e-129 375
MsG0580025512.01.T01 MTR_5g064890 44.560 193 88 8 18 198 20 205 2.01e-44 157
MsG0580025512.01.T01 MTR_4g093830 41.848 184 93 5 17 192 20 197 1.80e-39 144
MsG0580025512.01.T01 MTR_2g009310 33.746 323 182 10 60 364 65 373 5.23e-37 138
MsG0580025512.01.T01 MTR_8g089880 38.462 195 92 4 37 204 17 210 7.36e-35 129
MsG0580025512.01.T01 MTR_1g043410 29.720 286 148 9 34 288 11 274 1.56e-30 119
MsG0580025512.01.T01 MTR_1g074190 31.707 287 148 11 70 322 53 325 9.74e-28 112
MsG0580025512.01.T01 MTR_4g106680 29.595 321 177 10 34 341 15 299 3.26e-25 104
MsG0580025512.01.T01 MTR_5g034790 29.773 309 186 10 34 333 20 306 4.97e-25 105
MsG0580025512.01.T01 MTR_1g070140 28.483 323 174 15 34 326 25 320 1.45e-24 103
MsG0580025512.01.T01 MTR_4g129420 38.824 170 84 5 102 251 98 267 3.07e-23 101
MsG0580025512.01.T01 MTR_6g007737 38.095 147 89 2 47 192 59 204 3.38e-23 100
MsG0580025512.01.T01 MTR_6g087150 34.395 157 86 3 49 198 31 177 1.13e-22 98.6
MsG0580025512.01.T01 MTR_7g096690 27.619 315 183 11 58 340 13 314 2.51e-21 94.0
MsG0580025512.01.T01 MTR_8g063660 34.054 185 113 3 58 235 4 186 3.36e-21 93.6
MsG0580025512.01.T01 MTR_3g099650 29.213 178 99 4 39 203 29 192 6.60e-21 93.6
MsG0580025512.01.T01 MTR_3g464780 48.113 106 49 4 104 203 83 188 6.84e-21 91.3
MsG0580025512.01.T01 MTR_7g113800 34.868 152 77 4 48 192 29 165 8.81e-21 92.8
MsG0580025512.01.T01 MTR_3g110190 40.179 112 56 3 121 225 66 173 9.08e-21 92.4
MsG0580025512.01.T01 MTR_5g064980 35.766 137 77 3 59 192 54 182 2.37e-20 92.0
MsG0580025512.01.T01 MTR_1g082550 30.570 193 96 5 56 245 46 203 2.41e-20 91.7
MsG0580025512.01.T01 MTR_3g091620 52.000 75 36 0 119 193 99 173 3.87e-20 90.9
MsG0580025512.01.T01 MTR_3g094000 41.304 92 52 1 116 205 86 177 5.90e-20 86.7
MsG0580025512.01.T01 MTR_5g066250 35.417 144 81 2 49 192 74 205 1.48e-19 90.1
MsG0580025512.01.T01 MTR_4g063070 33.766 154 89 6 54 200 242 389 3.36e-19 88.6
MsG0580025512.01.T01 MTR_5g064990 29.897 194 117 5 41 229 48 227 4.81e-19 88.2
MsG0580025512.01.T01 MTR_4g120270 48.684 76 37 1 119 194 78 151 6.60e-19 86.3
MsG0580025512.01.T01 MTR_3g100400 30.061 163 103 3 37 192 43 201 1.00e-18 87.4
MsG0580025512.01.T01 MTR_7g076250 34.595 185 91 9 58 235 8 169 1.02e-18 83.6
MsG0580025512.01.T01 MTR_4g056590 38.614 101 57 2 120 220 99 194 8.87e-18 81.3
MsG0580025512.01.T01 MTR_3g071300 30.657 137 78 2 60 192 28 151 1.07e-17 80.5
MsG0580025512.01.T01 MTR_5g026730 26.289 194 116 5 48 239 4 172 1.11e-17 80.5
MsG0580025512.01.T01 MTR_7g073200 38.144 97 60 0 116 212 76 172 1.27e-17 80.5
MsG0580025512.01.T01 MTR_4g022410 33.333 186 88 8 34 214 21 175 1.56e-17 80.5
MsG0580025512.01.T01 MTR_3g078470 33.094 139 80 4 56 192 22 149 1.73e-17 80.9
MsG0580025512.01.T01 MTR_2g436100 34.286 140 87 2 119 253 91 230 2.51e-17 82.0
MsG0580025512.01.T01 MTR_2g090905 35.537 121 66 3 78 192 2 116 2.82e-17 77.8
MsG0580025512.01.T01 MTR_5g082940 40.000 95 47 3 106 192 56 148 3.24e-17 79.0
MsG0580025512.01.T01 MTR_7g116220 47.436 78 34 2 120 192 69 144 7.76e-17 78.2
MsG0580025512.01.T01 MTR_8g023400 48.611 72 36 1 121 192 92 162 7.84e-17 80.9
MsG0580025512.01.T01 MTR_8g073120 30.137 146 82 3 47 190 14 141 2.04e-16 77.4
MsG0580025512.01.T01 MTR_5g026720 28.667 150 85 4 48 191 4 137 2.68e-16 75.9
MsG0580025512.01.T01 MTR_3g086700 39.252 107 52 2 146 250 74 169 3.21e-16 76.6
MsG0580025512.01.T01 MTR_7g116180 37.607 117 60 3 120 233 66 172 4.05e-16 76.3
MsG0580025512.01.T01 MTR_8g093010 32.857 140 75 4 55 193 59 180 4.23e-16 77.0
MsG0580025512.01.T01 MTR_2g055790 28.495 186 98 6 49 225 25 184 4.25e-16 76.6
MsG0580025512.01.T01 MTR_2g096350 39.241 79 46 1 117 193 103 181 5.45e-16 76.6
MsG0580025512.01.T01 MTR_1g070425 45.570 79 40 1 120 195 67 145 5.56e-16 75.9
MsG0580025512.01.T01 MTR_4g030070 34.906 106 64 2 104 209 72 172 5.65e-16 76.6
MsG0580025512.01.T01 MTR_8g092970 44.578 83 45 1 145 227 129 210 7.92e-16 76.3
MsG0580025512.01.T01 MTR_1g492830 31.333 150 82 4 58 201 8 142 9.32e-16 76.3
MsG0580025512.01.T01 MTR_3g086730 38.261 115 52 4 146 250 74 179 9.61e-16 76.3
MsG0580025512.01.T01 MTR_2g044570 47.692 65 34 0 130 194 66 130 9.68e-16 77.4
MsG0580025512.01.T01 MTR_3g095560 30.288 208 107 8 37 227 34 220 9.74e-16 76.3
MsG0580025512.01.T01 MTR_1g100737 38.750 80 44 1 118 192 89 168 1.03e-15 75.5
MsG0580025512.01.T01 MTR_8g092840 40.506 79 46 1 116 193 108 186 1.20e-15 75.9
MsG0580025512.01.T01 MTR_7g117540 45.205 73 39 1 122 194 77 148 1.60e-15 76.6
MsG0580025512.01.T01 MTR_8g092960 41.558 77 44 1 118 193 100 176 1.86e-15 75.1
MsG0580025512.01.T01 MTR_5g024730 27.950 161 85 4 34 192 19 150 2.16e-15 74.7
MsG0580025512.01.T01 MTR_8g071815 31.544 149 77 3 48 190 4 133 3.41e-15 73.6
MsG0580025512.01.T01 MTR_3g078540 30.769 143 68 5 55 191 6 123 3.50e-15 72.8
MsG0580025512.01.T01 MTR_2g041200 32.609 138 72 4 60 192 33 154 5.14e-15 72.8
MsG0580025512.01.T01 MTR_7g116210 50.000 70 34 1 121 190 10 78 6.87e-15 70.9
MsG0580025512.01.T01 MTR_7g079280 42.254 71 39 1 121 189 80 150 7.68e-15 72.4
MsG0580025512.01.T01 MTR_8g028465 46.377 69 35 1 121 189 90 156 8.31e-15 72.8
MsG0580025512.01.T01 MTR_8g009190 30.675 163 92 5 36 193 5 151 9.14e-15 74.7
MsG0580025512.01.T01 MTR_8g092990 45.714 70 36 1 145 212 139 208 9.97e-15 73.2
MsG0580025512.01.T01 MTR_3g078450 33.871 124 71 3 71 192 35 149 1.40e-14 72.8
MsG0580025512.01.T01 MTR_7g116200 63.636 44 16 0 147 190 93 136 1.75e-14 71.2
MsG0580025512.01.T01 MTR_2g090950 37.037 81 45 1 118 192 88 168 1.77e-14 72.8
MsG0580025512.01.T01 MTR_3g114500 55.769 52 23 0 141 192 65 116 1.90e-14 70.1
MsG0580025512.01.T01 MTR_3g012550 31.098 164 87 6 58 213 23 168 2.50e-14 70.9
MsG0580025512.01.T01 MTR_5g022920 62.222 45 17 0 145 189 187 231 2.58e-14 72.0
MsG0580025512.01.T01 MTR_8g092985 40.000 75 44 1 120 193 110 184 3.58e-14 71.2
MsG0580025512.01.T01 MTR_8g092930 40.000 75 44 1 120 193 111 185 3.59e-14 71.6
MsG0580025512.01.T01 MTR_8g019710 54.902 51 23 0 142 192 49 99 4.55e-14 69.3
MsG0580025512.01.T01 MTR_3g116000 52.941 51 24 0 142 192 99 149 4.62e-14 70.5
MsG0580025512.01.T01 MTR_5g092790 37.179 78 47 1 117 192 153 230 4.91e-14 71.2
MsG0580025512.01.T01 MTR_7g108855 43.836 73 39 1 122 192 67 139 6.60e-14 70.1
MsG0580025512.01.T01 MTR_8g074900 60.000 45 18 0 145 189 178 222 9.83e-14 70.5
MsG0580025512.01.T01 MTR_5g092750 54.348 46 21 0 147 192 185 230 1.02e-13 70.5
MsG0580025512.01.T01 MTR_3g078520 47.619 63 30 1 129 191 88 147 1.10e-13 68.9
MsG0580025512.01.T01 MTR_6g055030 55.319 47 21 0 146 192 125 171 1.66e-13 71.2
MsG0580025512.01.T01 MTR_5g071930 36.111 108 59 4 104 210 54 152 2.30e-13 68.6
MsG0580025512.01.T01 MTR_8g027980 53.030 66 27 2 146 209 116 179 2.48e-13 69.7
MsG0580025512.01.T01 MTR_8g032690 36.364 77 48 1 144 219 16 92 2.60e-13 66.2
MsG0580025512.01.T01 MTR_2g035800 54.167 48 22 0 145 192 100 147 9.61e-13 66.2
MsG0580025512.01.T01 MTR_6g055030 50.909 55 22 1 143 192 86 140 1.03e-12 68.2
MsG0580025512.01.T01 MTR_7g059360 35.099 151 83 4 45 192 65 203 1.04e-12 67.4
MsG0580025512.01.T01 MTR_8g099515 31.081 148 85 4 63 205 260 395 1.31e-12 68.9
MsG0580025512.01.T01 MTR_5g009210 34.307 137 73 5 55 189 236 357 1.33e-12 68.6
MsG0580025512.01.T01 MTR_1g056350 40.964 83 45 2 113 191 65 147 1.68e-12 65.5
MsG0580025512.01.T01 MTR_1g090480 29.655 145 89 4 49 189 9 144 3.65e-12 64.7
MsG0580025512.01.T01 MTR_4g094725 60.465 43 17 0 147 189 106 148 5.09e-12 63.9
MsG0580025512.01.T01 MTR_8g099495 33.028 109 64 2 83 189 288 389 5.39e-12 67.0
MsG0580025512.01.T01 MTR_8g072020 45.333 75 38 3 125 197 383 456 6.59e-12 67.0
MsG0580025512.01.T01 MTR_5g076990 39.744 78 43 1 119 192 74 151 6.60e-12 66.2
MsG0580025512.01.T01 MTR_8g072020 45.333 75 38 3 125 197 422 495 6.65e-12 67.0
MsG0580025512.01.T01 MTR_7g079290 33.750 80 49 1 118 193 122 201 1.24e-11 63.9
MsG0580025512.01.T01 MTR_3g078120 37.179 78 43 2 136 210 77 151 1.66e-11 62.4
MsG0580025512.01.T01 MTR_1g026070 48.936 47 24 0 146 192 70 116 3.92e-11 60.8
MsG0580025512.01.T01 MTR_5g009200 41.667 84 44 3 109 189 311 392 3.92e-11 64.3
MsG0580025512.01.T01 MTR_5g009190 39.759 83 47 2 109 189 255 336 3.99e-11 63.9
MsG0580025512.01.T01 MTR_8g066740 48.936 47 24 0 146 192 105 151 9.92e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025512.01.T01 AT5G17600 42.368 380 189 12 6 376 4 362 2.05e-71 228
MsG0580025512.01.T01 AT3G03550 54.825 228 89 4 1 216 1 226 2.26e-69 222
MsG0580025512.01.T01 AT4G17905 36.148 379 157 14 1 370 1 303 2.48e-60 197
MsG0580025512.01.T01 AT4G17905 36.675 379 155 14 1 370 27 329 5.48e-60 197
MsG0580025512.01.T01 AT1G72220 37.008 254 119 8 18 240 22 265 1.22e-41 151
MsG0580025512.01.T01 AT4G33565 38.785 214 94 5 112 324 182 359 6.93e-33 126
MsG0580025512.01.T01 AT1G04360 40.000 190 93 6 46 224 31 210 1.13e-32 126
MsG0580025512.01.T01 AT5G10380 38.222 225 92 7 111 326 99 285 2.15e-32 124
MsG0580025512.01.T01 AT5G43420 31.741 293 135 11 51 290 30 310 2.04e-29 117
MsG0580025512.01.T01 AT1G23980 39.583 144 82 3 56 195 50 192 1.23e-28 115
MsG0580025512.01.T01 AT5G40250 31.727 249 136 6 56 287 42 273 2.36e-25 106
MsG0580025512.01.T01 AT1G72200 31.429 175 95 5 52 218 56 213 1.39e-23 101
MsG0580025512.01.T01 AT2G18650 42.453 106 60 1 90 194 68 173 4.93e-22 97.4
MsG0580025512.01.T01 AT5G57750 33.333 156 96 3 56 206 33 185 1.75e-21 92.0
MsG0580025512.01.T01 AT4G28890 37.255 153 88 2 37 189 21 165 1.84e-21 95.5
MsG0580025512.01.T01 AT3G48030 36.842 171 86 5 48 208 110 268 3.21e-21 94.0
MsG0580025512.01.T01 AT3G48030 36.842 171 86 5 48 208 32 190 3.52e-21 92.4
MsG0580025512.01.T01 AT3G18930 30.455 220 107 6 55 245 38 240 4.27e-21 94.4
MsG0580025512.01.T01 AT3G18930 30.455 220 107 6 55 245 38 240 4.27e-21 94.4
MsG0580025512.01.T01 AT3G18930 30.455 220 107 6 55 245 38 240 4.27e-21 94.4
MsG0580025512.01.T01 AT4G30400 46.939 98 50 2 93 189 80 176 1.14e-20 93.6
MsG0580025512.01.T01 AT3G05200 30.625 160 88 4 37 192 33 173 6.56e-20 90.9
MsG0580025512.01.T01 AT2G20030 32.704 159 89 4 38 192 25 169 7.94e-20 90.5
MsG0580025512.01.T01 AT3G16720 33.333 159 95 3 108 257 81 237 9.78e-20 89.0
MsG0580025512.01.T01 AT5G05810 32.624 141 95 0 52 192 51 191 2.69e-19 89.0
MsG0580025512.01.T01 AT5G01880 42.500 80 46 0 121 200 79 158 4.40e-19 84.0
MsG0580025512.01.T01 AT1G22500 31.677 161 94 4 45 202 26 173 7.34e-19 87.4
MsG0580025512.01.T01 AT4G40070 32.075 159 85 6 40 194 32 171 2.00e-18 85.5
MsG0580025512.01.T01 AT1G35330 46.154 78 39 1 118 192 97 174 5.74e-18 84.3
MsG0580025512.01.T01 AT4G15975 47.368 76 39 1 120 195 50 124 6.40e-18 82.4
MsG0580025512.01.T01 AT1G49230 28.497 193 115 5 37 225 39 212 7.25e-18 82.0
MsG0580025512.01.T01 AT3G10910 44.872 78 39 2 118 193 85 160 1.03e-17 80.5
MsG0580025512.01.T01 AT4G09100 46.053 76 38 1 120 192 55 130 1.20e-17 79.0
MsG0580025512.01.T01 AT2G35000 32.117 137 83 3 59 192 51 180 1.66e-17 83.6
MsG0580025512.01.T01 AT1G53820 44.578 83 43 2 110 192 86 165 2.97e-17 82.0
MsG0580025512.01.T01 AT2G34990 33.571 140 81 3 56 192 11 141 3.99e-17 81.6
MsG0580025512.01.T01 AT2G34990 33.571 140 81 3 56 192 33 163 4.15e-17 81.6
MsG0580025512.01.T01 AT4G09110 30.811 185 97 5 121 291 94 261 4.57e-17 81.3
MsG0580025512.01.T01 AT1G76410 29.655 145 88 4 50 193 19 150 5.11e-17 79.0
MsG0580025512.01.T01 AT5G27420 28.421 190 98 6 47 215 28 200 8.74e-17 81.3
MsG0580025512.01.T01 AT3G62690 46.479 71 38 0 122 192 88 158 1.81e-16 79.0
MsG0580025512.01.T01 AT4G10160 33.333 132 78 4 121 244 72 201 2.07e-16 78.2
MsG0580025512.01.T01 AT5G66070 53.571 56 26 0 134 189 163 218 4.97e-16 77.0
MsG0580025512.01.T01 AT5G66070 53.571 56 26 0 134 189 163 218 4.97e-16 77.0
MsG0580025512.01.T01 AT2G47560 45.333 75 39 1 120 192 79 153 6.08e-16 76.6
MsG0580025512.01.T01 AT2G17730 38.889 108 57 3 88 192 140 241 7.11e-16 77.0
MsG0580025512.01.T01 AT5G66070 53.571 56 26 0 134 189 187 242 1.01e-15 76.6
MsG0580025512.01.T01 AT5G05280 43.836 73 40 1 121 193 88 159 1.32e-15 74.7
MsG0580025512.01.T01 AT1G72310 40.476 84 49 1 117 200 98 180 1.67e-15 77.0
MsG0580025512.01.T01 AT4G09120 44.000 75 39 1 121 192 94 168 1.73e-15 77.4
MsG0580025512.01.T01 AT4G10150 34.146 123 73 4 121 237 86 206 3.32e-15 74.7
MsG0580025512.01.T01 AT2G27940 40.845 71 41 1 120 190 114 183 4.02e-15 74.7
MsG0580025512.01.T01 AT2G42360 28.986 138 84 3 56 192 29 153 4.75e-15 74.3
MsG0580025512.01.T01 AT2G35420 36.441 118 55 4 116 217 68 181 1.49e-14 73.2
MsG0580025512.01.T01 AT2G35910 34.959 123 75 1 80 202 85 202 1.54e-14 72.4
MsG0580025512.01.T01 AT1G32361 46.377 69 35 1 121 189 130 196 2.77e-14 73.6
MsG0580025512.01.T01 AT4G09130 42.045 88 41 3 112 192 81 165 2.99e-14 73.6
MsG0580025512.01.T01 AT2G46495 32.680 153 78 4 46 189 226 362 3.69e-14 73.6
MsG0580025512.01.T01 AT1G33480 35.238 105 62 3 121 225 75 173 3.89e-14 72.4
MsG0580025512.01.T01 AT1G20823 26.623 154 101 4 40 192 14 156 4.69e-14 70.9
MsG0580025512.01.T01 AT4G35840 44.000 75 39 1 118 189 159 233 5.61e-14 71.2
MsG0580025512.01.T01 AT2G17450 29.412 153 87 3 37 189 13 144 5.83e-14 70.1
MsG0580025512.01.T01 AT1G28040 30.573 157 72 6 47 189 162 295 6.23e-14 72.0
MsG0580025512.01.T01 AT5G06490 29.870 154 76 4 63 191 27 173 7.18e-14 70.1
MsG0580025512.01.T01 AT3G60220 57.143 49 21 0 146 194 116 164 7.26e-14 72.4
MsG0580025512.01.T01 AT4G35480 60.000 45 18 0 145 189 111 155 1.67e-13 69.3
MsG0580025512.01.T01 AT2G46495 43.210 81 33 2 117 189 259 334 2.12e-13 71.2
MsG0580025512.01.T01 AT3G18773 35.780 109 67 2 120 225 102 210 2.21e-13 69.3
MsG0580025512.01.T01 AT2G42350 45.070 71 36 2 121 190 75 143 2.66e-13 68.9
MsG0580025512.01.T01 AT1G49200 30.921 152 86 5 40 189 44 178 3.33e-13 68.9
MsG0580025512.01.T01 AT3G61550 36.364 88 45 1 113 189 91 178 3.88e-13 68.6
MsG0580025512.01.T01 AT3G14320 40.278 72 42 1 121 192 63 133 4.80e-13 68.2
MsG0580025512.01.T01 AT2G46493 37.349 83 47 3 111 189 99 180 5.21e-13 67.4
MsG0580025512.01.T01 AT5G07040 31.343 134 71 5 63 189 17 136 5.82e-13 66.6
MsG0580025512.01.T01 AT1G49220 37.805 82 50 1 109 189 96 177 6.42e-13 68.6
MsG0580025512.01.T01 AT5G47610 51.020 49 24 0 146 194 106 154 6.80e-13 66.6
MsG0580025512.01.T01 AT2G37580 41.558 77 44 1 117 192 111 187 8.08e-13 67.8
MsG0580025512.01.T01 AT1G49210 39.437 71 42 1 120 189 107 177 1.25e-12 67.4
MsG0580025512.01.T01 AT2G46494 30.370 135 77 4 59 189 237 358 1.29e-12 68.9
MsG0580025512.01.T01 AT2G25410 44.048 84 32 3 116 190 293 370 1.33e-12 68.9
MsG0580025512.01.T01 AT1G74410 48.980 49 25 0 144 192 173 221 1.68e-12 66.6
MsG0580025512.01.T01 AT3G19140 37.363 91 55 2 102 192 36 124 3.55e-12 63.9
MsG0580025512.01.T01 AT2G46160 37.662 77 40 1 121 189 104 180 3.73e-12 65.5
MsG0580025512.01.T01 AT2G46160 37.662 77 40 1 121 189 104 180 3.73e-12 65.5
MsG0580025512.01.T01 AT5G42200 50.000 48 24 0 145 192 102 149 7.22e-12 63.5
MsG0580025512.01.T01 AT4G17920 29.677 155 95 7 41 189 6 152 1.15e-11 65.5
MsG0580025512.01.T01 AT2G18670 36.842 76 47 1 118 192 80 155 1.32e-11 63.2
MsG0580025512.01.T01 AT4G30370 35.065 77 50 0 119 195 87 163 1.33e-11 63.2
MsG0580025512.01.T01 AT4G17245 45.833 72 31 3 146 215 101 166 1.55e-11 62.8
MsG0580025512.01.T01 AT5G58580 34.545 110 68 2 121 229 111 217 1.87e-11 65.1
MsG0580025512.01.T01 AT2G17730 37.755 98 52 3 88 182 140 231 2.05e-11 63.9
MsG0580025512.01.T01 AT5G53110 33.588 131 74 4 61 189 253 372 2.53e-11 65.1
MsG0580025512.01.T01 AT1G51930 52.174 46 22 0 147 192 80 125 3.25e-11 60.8
MsG0580025512.01.T01 AT5G46650 29.730 148 81 7 57 192 24 160 3.56e-11 63.9
MsG0580025512.01.T01 AT3G60966 56.522 46 20 0 147 192 62 107 5.22e-11 60.5
MsG0580025512.01.T01 AT3G11110 38.667 75 45 1 119 192 78 152 8.79e-11 60.5

Find 64 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACAGTACTTTGATATTA+TGG 0.213148 5:+19850298 None:intergenic
CAATCTTTGCTGTTCATATA+TGG 0.238266 5:+19850494 None:intergenic
GTCGATGTTGAAAGAGAAAA+TGG 0.250803 5:-19849837 MsG0580025512.01.T01:CDS
CGAAATTCTGTCTTCGGAAT+TGG 0.278129 5:+19849792 None:intergenic
ATTTCGTGCTTTAAGCGATT+TGG 0.294763 5:-19849775 MsG0580025512.01.T01:CDS
AAAGAAACTTCATTCGTTGA+TGG 0.297827 5:+19849885 None:intergenic
CCTCTTGTCATTGCAATCAT+TGG 0.318007 5:-19850359 MsG0580025512.01.T01:CDS
AATGATCTCTTCATTGCAAT+TGG 0.323935 5:+19849483 None:intergenic
ATATATGGAACCCAAGCTTT+TGG 0.326959 5:+19850509 None:intergenic
TTTCGTGCTTTAAGCGATTT+GGG 0.328808 5:-19849774 MsG0580025512.01.T01:CDS
TGAAAAGCATGGCTACATTT+AGG 0.329173 5:+19850038 None:intergenic
ATGACAAGAGGAGAGAAATT+TGG 0.339314 5:+19850371 None:intergenic
TGAAGCGTTGAAGGCGAATA+TGG 0.343293 5:+19849956 None:intergenic
TTTGTATTTGCAAACTGTTA+TGG 0.359980 5:+19850142 None:intergenic
TTGAAACATGCCATGGTTCA+TGG 0.360551 5:+19850195 None:intergenic
CCATGGCATGTTTCAACAAA+TGG 0.374002 5:-19850188 MsG0580025512.01.T01:CDS
CTCTTGGAAATCCAAAAGCT+TGG 0.375567 5:-19850520 MsG0580025512.01.T01:CDS
TTTGGACTTGAATCTTCATC+AGG 0.383193 5:+19850389 None:intergenic
CAAATGTAGTTATAGAAGAA+AGG 0.410341 5:-19849526 MsG0580025512.01.T01:CDS
ACTTGAACTTGAAGCGTTGA+AGG 0.414135 5:+19849947 None:intergenic
TATTGTACTATGGCTTCTCT+TGG 0.426468 5:-19850536 None:intergenic
TCTTGGAAATCCAAAAGCTT+GGG 0.428511 5:-19850519 MsG0580025512.01.T01:CDS
AAAGCACGAAATTCTGTCTT+CGG 0.430339 5:+19849786 None:intergenic
GGTGAAAGTGAAAGTGAAAG+TGG 0.439110 5:-19849555 MsG0580025512.01.T01:CDS
AGCGATTTGGGAAATTTAAG+AGG 0.447948 5:-19849762 MsG0580025512.01.T01:CDS
AAGGGAGAAGGAGAAGGTGG+AGG 0.449613 5:+19850419 None:intergenic
ATCTTCCATGTATTGATACA+TGG 0.451685 5:-19850016 MsG0580025512.01.T01:CDS
GGTTCATCAAAGAGGTTAAG+AGG 0.469232 5:-19849576 MsG0580025512.01.T01:CDS
AGGGAGAAGGAGAAGGTGGA+GGG 0.470684 5:+19850420 None:intergenic
TCAAAGGGAGAAGGAGAAGG+TGG 0.474436 5:+19850416 None:intergenic
AACAGCAAAGATTGTTCTCA+AGG 0.476931 5:-19850485 MsG0580025512.01.T01:CDS
TTCCTTAGCAGAAGTGGAAG+AGG 0.482749 5:-19849444 MsG0580025512.01.T01:CDS
GAACAAGATTCTTCTTCTCA+AGG 0.503397 5:-19849603 MsG0580025512.01.T01:CDS
ACAATTTCGTTCAAATGTTG+AGG 0.508817 5:+19849858 None:intergenic
TGCCATGGTTCATGGATTGA+AGG 0.509549 5:+19850203 None:intergenic
GATTCTTCTTCTCAAGGTGA+AGG 0.514978 5:-19849597 MsG0580025512.01.T01:CDS
AGAAGTGGAAGAGGAAGGCT+TGG 0.516144 5:-19849435 MsG0580025512.01.T01:CDS
TTCCTCTTCCACTTCTGCTA+AGG 0.523273 5:+19849442 None:intergenic
AATTCAAAGGGAGAAGGAGA+AGG 0.529200 5:+19850413 None:intergenic
TCTTCATCAGGGAATTCAAA+GGG 0.533476 5:+19850401 None:intergenic
ATTTGGGAAATTTAAGAGGA+AGG 0.540826 5:-19849758 MsG0580025512.01.T01:CDS
TGTTGAAAGAGAAAATGGAT+TGG 0.545432 5:-19849832 MsG0580025512.01.T01:CDS
CTGTGGTCGCAGAGAATCAT+CGG 0.546315 5:-19850282 MsG0580025512.01.T01:CDS
GCCATGGTTCATGGATTGAA+GGG 0.555580 5:+19850204 None:intergenic
TTGAGCCATGTATCAATACA+TGG 0.560560 5:+19850011 None:intergenic
CCATTTGTTGAAACATGCCA+TGG 0.561652 5:+19850188 None:intergenic
CCAATGATTGCAATGACAAG+AGG 0.570515 5:+19850359 None:intergenic
TGATGGATTGGTTGATGTAA+CGG 0.572425 5:-19850114 MsG0580025512.01.T01:CDS
ATCTTCATCAGGGAATTCAA+AGG 0.579122 5:+19850400 None:intergenic
TTGGACTTGAATCTTCATCA+GGG 0.582191 5:+19850390 None:intergenic
GGAAATTTAAGAGGAAGGCA+TGG 0.585730 5:-19849753 MsG0580025512.01.T01:CDS
TTAGCAGAAGTGGAAGAGGA+AGG 0.594760 5:-19849440 MsG0580025512.01.T01:CDS
TCAGGGAATTCAAAGGGAGA+AGG 0.600780 5:+19850407 None:intergenic
AGAAAATGGATTGGATGTGG+TGG 0.605576 5:-19849823 MsG0580025512.01.T01:CDS
ATGGATCATTCATTTCAAAG+TGG 0.610339 5:-19849669 MsG0580025512.01.T01:CDS
TAGATTACGTAGCACCATTG+TGG 0.619510 5:+19850443 None:intergenic
AATATGGTAGCGCGGCAAAG+AGG 0.626932 5:+19849972 None:intergenic
AAGAGAAAATGGATTGGATG+TGG 0.642882 5:-19849826 MsG0580025512.01.T01:CDS
AAGGTGAAGGTTCATCAAAG+AGG 0.650314 5:-19849584 MsG0580025512.01.T01:CDS
GTAGCGTCTATTGTCCACAA+TGG 0.651497 5:-19850457 MsG0580025512.01.T01:CDS
ACCCTTCAATCCATGAACCA+TGG 0.655334 5:-19850205 MsG0580025512.01.T01:CDS
TACATGGAAGATGAAAAGCA+TGG 0.666064 5:+19850027 None:intergenic
TGAAGGCGAATATGGTAGCG+CGG 0.688727 5:+19849964 None:intergenic
ACCATAATATCAAAGTACTG+TGG 0.714267 5:-19850299 MsG0580025512.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAATGTAGTTATAGAAGAA+AGG - Chr5:19850420-19850439 MsG0580025512.01.T01:CDS 25.0%
! TGCAAATACAAAAAGAATGA+TGG - Chr5:19849815-19849834 MsG0580025512.01.T01:CDS 25.0%
! TTTGTATTTGCAAACTGTTA+TGG + Chr5:19849807-19849826 None:intergenic 25.0%
!!! ATACAAAAAGAATGATGGAT+TGG - Chr5:19849820-19849839 MsG0580025512.01.T01:CDS 25.0%
AAAGAAACTTCATTCGTTGA+TGG + Chr5:19850064-19850083 None:intergenic 30.0%
AATGATCTCTTCATTGCAAT+TGG + Chr5:19850466-19850485 None:intergenic 30.0%
ACAATTTCGTTCAAATGTTG+AGG + Chr5:19850091-19850110 None:intergenic 30.0%
ACCACAGTACTTTGATATTA+TGG + Chr5:19849651-19849670 None:intergenic 30.0%
ATGGATCATTCATTTCAAAG+TGG - Chr5:19850277-19850296 MsG0580025512.01.T01:CDS 30.0%
ATTTGGGAAATTTAAGAGGA+AGG - Chr5:19850188-19850207 MsG0580025512.01.T01:CDS 30.0%
TGTTGAAAGAGAAAATGGAT+TGG - Chr5:19850114-19850133 MsG0580025512.01.T01:CDS 30.0%
! ATCTTCCATGTATTGATACA+TGG - Chr5:19849930-19849949 MsG0580025512.01.T01:CDS 30.0%
!! ACCATAATATCAAAGTACTG+TGG - Chr5:19849647-19849666 MsG0580025512.01.T01:CDS 30.0%
!! ATGAAGAGATCATTTTCAAG+TGG - Chr5:19850472-19850491 MsG0580025512.01.T01:CDS 30.0%
!! CAATCTTTGCTGTTCATATA+TGG + Chr5:19849455-19849474 None:intergenic 30.0%
AAAGCACGAAATTCTGTCTT+CGG + Chr5:19850163-19850182 None:intergenic 35.0%
AACAGCAAAGATTGTTCTCA+AGG - Chr5:19849461-19849480 MsG0580025512.01.T01:CDS 35.0%
ATCTTCATCAGGGAATTCAA+AGG + Chr5:19849549-19849568 None:intergenic 35.0%
ATGACAAGAGGAGAGAAATT+TGG + Chr5:19849578-19849597 None:intergenic 35.0%
GAACAAGATTCTTCTTCTCA+AGG - Chr5:19850343-19850362 MsG0580025512.01.T01:CDS 35.0%
GTCGATGTTGAAAGAGAAAA+TGG - Chr5:19850109-19850128 MsG0580025512.01.T01:CDS 35.0%
TACATGGAAGATGAAAAGCA+TGG + Chr5:19849922-19849941 None:intergenic 35.0%
TCTTCATCAGGGAATTCAAA+GGG + Chr5:19849548-19849567 None:intergenic 35.0%
TCTTGGAAATCCAAAAGCTT+GGG - Chr5:19849427-19849446 MsG0580025512.01.T01:CDS 35.0%
TTGAGCCATGTATCAATACA+TGG + Chr5:19849938-19849957 None:intergenic 35.0%
TTGGACTTGAATCTTCATCA+GGG + Chr5:19849559-19849578 None:intergenic 35.0%
TTTGGACTTGAATCTTCATC+AGG + Chr5:19849560-19849579 None:intergenic 35.0%
! AGATTTTTCCTTAGCAGAAG+TGG - Chr5:19850496-19850515 MsG0580025512.01.T01:CDS 35.0%
! AGCGATTTGGGAAATTTAAG+AGG - Chr5:19850184-19850203 MsG0580025512.01.T01:CDS 35.0%
! ATATATGGAACCCAAGCTTT+TGG + Chr5:19849440-19849459 None:intergenic 35.0%
! ATTTCGTGCTTTAAGCGATT+TGG - Chr5:19850171-19850190 MsG0580025512.01.T01:CDS 35.0%
! TGAAAAGCATGGCTACATTT+AGG + Chr5:19849911-19849930 None:intergenic 35.0%
! TTTCGTGCTTTAAGCGATTT+GGG - Chr5:19850172-19850191 MsG0580025512.01.T01:CDS 35.0%
!! AAGAGAAAATGGATTGGATG+TGG - Chr5:19850120-19850139 MsG0580025512.01.T01:CDS 35.0%
!! TGATGGATTGGTTGATGTAA+CGG - Chr5:19849832-19849851 MsG0580025512.01.T01:CDS 35.0%
!!! GTTGATGGATTTTCTGTAAC+AGG + Chr5:19850049-19850068 None:intergenic 35.0%
AAGGTGAAGGTTCATCAAAG+AGG - Chr5:19850362-19850381 MsG0580025512.01.T01:CDS 40.0%
AATTCAAAGGGAGAAGGAGA+AGG + Chr5:19849536-19849555 None:intergenic 40.0%
CCAATGATTGCAATGACAAG+AGG + Chr5:19849590-19849609 None:intergenic 40.0%
CCATGGCATGTTTCAACAAA+TGG - Chr5:19849758-19849777 MsG0580025512.01.T01:CDS 40.0%
CCTCTTGTCATTGCAATCAT+TGG - Chr5:19849587-19849606 MsG0580025512.01.T01:CDS 40.0%
CGAAATTCTGTCTTCGGAAT+TGG + Chr5:19850157-19850176 None:intergenic 40.0%
CTCTTGGAAATCCAAAAGCT+TGG - Chr5:19849426-19849445 MsG0580025512.01.T01:CDS 40.0%
GATTCTTCTTCTCAAGGTGA+AGG - Chr5:19850349-19850368 MsG0580025512.01.T01:CDS 40.0%
GGAAATTTAAGAGGAAGGCA+TGG - Chr5:19850193-19850212 MsG0580025512.01.T01:CDS 40.0%
GGTGAAAGTGAAAGTGAAAG+TGG - Chr5:19850391-19850410 MsG0580025512.01.T01:CDS 40.0%
GGTTCATCAAAGAGGTTAAG+AGG - Chr5:19850370-19850389 MsG0580025512.01.T01:CDS 40.0%
TAGATTACGTAGCACCATTG+TGG + Chr5:19849506-19849525 None:intergenic 40.0%
TTGAAACATGCCATGGTTCA+TGG + Chr5:19849754-19849773 None:intergenic 40.0%
! CCATTTGTTGAAACATGCCA+TGG + Chr5:19849761-19849780 None:intergenic 40.0%
! GATTCGACGATCGTTTTCTA+TGG - Chr5:19850258-19850277 MsG0580025512.01.T01:CDS 40.0%
!! ACTTGAACTTGAAGCGTTGA+AGG + Chr5:19850002-19850021 None:intergenic 40.0%
!! AGAAAATGGATTGGATGTGG+TGG - Chr5:19850123-19850142 MsG0580025512.01.T01:CDS 40.0%
ACCCTTCAATCCATGAACCA+TGG - Chr5:19849741-19849760 MsG0580025512.01.T01:CDS 45.0%
GTAGCGTCTATTGTCCACAA+TGG - Chr5:19849489-19849508 MsG0580025512.01.T01:CDS 45.0%
TCAGGGAATTCAAAGGGAGA+AGG + Chr5:19849542-19849561 None:intergenic 45.0%
TTAGCAGAAGTGGAAGAGGA+AGG - Chr5:19850506-19850525 MsG0580025512.01.T01:CDS 45.0%
TTCCTCTTCCACTTCTGCTA+AGG + Chr5:19850507-19850526 None:intergenic 45.0%
TTCCTTAGCAGAAGTGGAAG+AGG - Chr5:19850502-19850521 MsG0580025512.01.T01:CDS 45.0%
! GCCATGGTTCATGGATTGAA+GGG + Chr5:19849745-19849764 None:intergenic 45.0%
! GGATTTTCTGTAACAGGAGC+TGG + Chr5:19850043-19850062 None:intergenic 45.0%
! TGCCATGGTTCATGGATTGA+AGG + Chr5:19849746-19849765 None:intergenic 45.0%
!! TGAAGCGTTGAAGGCGAATA+TGG + Chr5:19849993-19850012 None:intergenic 45.0%
AGAAGTGGAAGAGGAAGGCT+TGG - Chr5:19850511-19850530 MsG0580025512.01.T01:CDS 50.0%
CTGTGGTCGCAGAGAATCAT+CGG - Chr5:19849664-19849683 MsG0580025512.01.T01:CDS 50.0%
TCAAAGGGAGAAGGAGAAGG+TGG + Chr5:19849533-19849552 None:intergenic 50.0%
! AATATGGTAGCGCGGCAAAG+AGG + Chr5:19849977-19849996 None:intergenic 50.0%
! TGAAGGCGAATATGGTAGCG+CGG + Chr5:19849985-19850004 None:intergenic 50.0%
AAGGGAGAAGGAGAAGGTGG+AGG + Chr5:19849530-19849549 None:intergenic 55.0%
AGGGAGAAGGAGAAGGTGGA+GGG + Chr5:19849529-19849548 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 19849419 19850549 19849419 ID=MsG0580025512.01;Name=MsG0580025512.01
Chr5 mRNA 19849419 19850549 19849419 ID=MsG0580025512.01.T01;Parent=MsG0580025512.01;Name=MsG0580025512.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|376
Chr5 exon 19849419 19850549 19849419 ID=MsG0580025512.01.T01:exon:8347;Parent=MsG0580025512.01.T01
Chr5 CDS 19849419 19850549 19849419 ID=MsG0580025512.01.T01:cds;Parent=MsG0580025512.01.T01
Gene Sequence

>MsG0580025512.01.T01

ATGGCTTCTCTTGGAAATCCAAAAGCTTGGGTTCCATATATGAACAGCAAAGATTGTTCTCAAGGTTTTTGTAGCGTCTATTGTCCACAATGGTGCTACGTAATCTACCCTCCACCTTCTCCTTCTCCCTTTGAATTCCCTGATGAAGATTCAAGTCCAAATTTCTCTCCTCTTGTCATTGCAATCATTGGAATCTTAGCAAGTGCTTTTCTTCTTGTAACTTACTACACCATAATATCAAAGTACTGTGGTCGCAGAGAATCATCGGTAAGTGAATCGCGTGAAGCAAACGATGAATTTGAAGATGATCATCATCATCATAACCCTTCAATCCATGAACCATGGCATGTTTCAACAAATGGATTAGATGAAAATTTGATTAAATCCATAACAGTTTGCAAATACAAAAAGAATGATGGATTGGTTGATGTAACGGATTGTTCAGTTTGTCTAAACGAGTTTCAAGACGATGAAAGTATTAGACTTTTACCTAAATGTAGCCATGCTTTTCATCTTCCATGTATTGATACATGGCTCAAATCTCATTCTAACTGTCCTCTTTGCCGCGCTACCATATTCGCCTTCAACGCTTCAAGTTCAAGTGCTGCAACACTTCATGTGCCAGCTCCTGTTACAGAAAATCCATCAACGAATGAAGTTTCTTTAGAACCTCAACATTTGAACGAAATTGTCGATGTTGAAAGAGAAAATGGATTGGATGTGGTGGAAGAAGTTCCAATTCCGAAGACAGAATTTCGTGCTTTAAGCGATTTGGGAAATTTAAGAGGAAGGCATGGTGTGATTGAGATTAGAGATCATAATGAAGATTATCGTGAATCGATTCGACGATCGTTTTCTATGGATCATTCATTTCAAAGTGGTGTTTTAGTTGTTGATGATGTTAATGTTATGCATATGAATCAAGAACAAGATTCTTCTTCTCAAGGTGAAGGTTCATCAAAGAGGTTAAGAGGTGAAAGTGAAAGTGAAAGTGGAAGTAGCAAATGTAGTTATAGAAGAAAGGTTTTGCATTGTGTTTTAAGTCCAATTGCAATGAAGAGATCATTTTCAAGTGGAAGATTTTTCCTTAGCAGAAGTGGAAGAGGAAGGCTTGGAGTTTTGCCAGTCTAA
Protein sequence

>MsG0580025512.01.T01

MASLGNPKAWVPYMNSKDCSQGFCSVYCPQWCYVIYPPPSPSPFEFPDEDSSPNFSPLVIAIIGILASAFLLVTYYTIISKYCGRRESSVSESREANDEFEDDHHHHNPSIHEPWHVSTNGLDENLIKSITVCKYKKNDGLVDVTDCSVCLNEFQDDESIRLLPKCSHAFHLPCIDTWLKSHSNCPLCRATIFAFNASSSSAATLHVPAPVTENPSTNEVSLEPQHLNEIVDVERENGLDVVEEVPIPKTEFRALSDLGNLRGRHGVIEIRDHNEDYRESIRRSFSMDHSFQSGVLVVDDVNVMHMNQEQDSSSQGEGSSKRLRGESESESGSSKCSYRRKVLHCVLSPIAMKRSFSSGRFFLSRSGRGRLGVLPV*