Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025839.01.T01 | XP_024639710.1 | 91.545 | 1100 | 93 | 0 | 1 | 1100 | 1 | 1100 | 0 | 2013 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180006054.01 | MsG0580025839.01 | 0.803655 | 3.142517e-49 | 1.587979e-46 |
| MsG0380017220.01 | MsG0580025839.01 | 0.805652 | 1.203560e-49 | 6.402794e-47 |
| MsG0580025839.01 | MsG0580025841.01 | 0.852759 | 3.739544e-61 | 7.895294e-58 |
| MsG0580025839.01 | MsG0880046439.01 | 0.845370 | 4.237963e-59 | 7.046696e-56 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025839.01.T01 | MTR_5g035280 | 91.636 | 1088 | 91 | 0 | 1 | 1088 | 1 | 1088 | 0.0 | 1988 |
| MsG0580025839.01.T01 | MTR_3g026660 | 69.150 | 1141 | 308 | 3 | 1 | 1097 | 1 | 1141 | 0.0 | 1566 |
| MsG0580025839.01.T01 | MTR_5g035240 | 75.692 | 975 | 235 | 2 | 1 | 975 | 1 | 973 | 0.0 | 1454 |
| MsG0580025839.01.T01 | MTR_3g026660 | 64.680 | 1141 | 302 | 5 | 1 | 1097 | 1 | 1084 | 0.0 | 1429 |
| MsG0580025839.01.T01 | MTR_5g037450 | 65.797 | 1111 | 365 | 5 | 1 | 1100 | 1 | 1107 | 0.0 | 1425 |
| MsG0580025839.01.T01 | MTR_5g037500 | 64.461 | 1103 | 268 | 12 | 1 | 1096 | 1 | 986 | 0.0 | 1267 |
| MsG0580025839.01.T01 | MTR_5g035530 | 61.735 | 980 | 365 | 3 | 122 | 1094 | 1 | 977 | 0.0 | 1193 |
| MsG0580025839.01.T01 | MTR_5g035480 | 59.195 | 1093 | 344 | 9 | 1 | 1088 | 1 | 996 | 0.0 | 1184 |
| MsG0580025839.01.T01 | MTR_5g034770 | 63.727 | 907 | 269 | 12 | 1 | 900 | 1 | 854 | 0.0 | 1061 |
| MsG0580025839.01.T01 | MTR_5g034770 | 66.038 | 106 | 36 | 0 | 983 | 1088 | 768 | 873 | 4.74e-28 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025839.01.T01 | AT3G14470 | 33.436 | 972 | 577 | 22 | 31 | 952 | 39 | 990 | 4.30e-145 | 463 |
| MsG0580025839.01.T01 | AT3G14470 | 33.436 | 972 | 577 | 22 | 31 | 952 | 39 | 990 | 4.30e-145 | 463 |
| MsG0580025839.01.T01 | AT3G14460 | 31.116 | 887 | 527 | 18 | 31 | 855 | 36 | 900 | 1.82e-114 | 387 |
| MsG0580025839.01.T01 | AT3G50950 | 29.913 | 809 | 487 | 24 | 1 | 768 | 1 | 770 | 1.74e-78 | 276 |
| MsG0580025839.01.T01 | AT3G50950 | 29.913 | 809 | 487 | 24 | 1 | 768 | 1 | 770 | 1.74e-78 | 276 |
| MsG0580025839.01.T01 | AT1G53350 | 30.199 | 702 | 427 | 20 | 1 | 663 | 1 | 678 | 8.97e-64 | 234 |
| MsG0580025839.01.T01 | AT3G07040 | 28.213 | 677 | 423 | 20 | 1 | 636 | 1 | 655 | 2.66e-63 | 233 |
| MsG0580025839.01.T01 | AT5G43470 | 30.280 | 644 | 384 | 16 | 1 | 603 | 1 | 620 | 2.27e-61 | 227 |
| MsG0580025839.01.T01 | AT5G43470 | 30.280 | 644 | 384 | 16 | 1 | 603 | 1 | 620 | 2.27e-61 | 227 |
| MsG0580025839.01.T01 | AT5G43470 | 30.280 | 644 | 384 | 16 | 1 | 603 | 1 | 620 | 2.27e-61 | 227 |
Find 219 sgRNAs with CRISPR-Local
Find 248 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGGTGTAAAGAGAAAAC+AGG | 0.506903 | 5:-24939617 | MsG0580025839.01.T01:CDS |
| AAATTGCTAAGAAAATGAAG+AGG | 0.672871 | 5:-24942478 | MsG0580025839.01.T01:CDS |
| AACATTCTTAGGATTTAAAG+AGG | 0.471285 | 5:+24942514 | None:intergenic |
| AACTAACTTGCTTTCCAGAT+GGG | 0.499249 | 5:-24940132 | MsG0580025839.01.T01:CDS |
| AACTCAGCAACTTCGAAGCC+TGG | 0.441177 | 5:-24940614 | MsG0580025839.01.T01:CDS |
| AAGAGAAAACAGGTGAGGAT+TGG | 0.513788 | 5:-24939607 | MsG0580025839.01.T01:CDS |
| AAGCAAGTGCGTGGACAAGT+TGG | 0.548969 | 5:-24940713 | MsG0580025839.01.T01:CDS |
| AAGCACTGGGAAGTTTATTG+CGG | 0.616162 | 5:-24941719 | MsG0580025839.01.T01:CDS |
| AAGGAGCTTGGCCTATTTCT+AGG | 0.250653 | 5:-24942773 | MsG0580025839.01.T01:CDS |
| AAGTAACTTAATCTCAATGC+AGG | 0.541024 | 5:+24941618 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTAACTTAATCTCAATGC+AGG | + | Chr5:24940759-24940778 | None:intergenic | 30.0% | |
| AGATACTGTTAGACTCTTTA+GGG | + | Chr5:24941915-24941934 | None:intergenic | 30.0% | |
| AGCAGTTACTAATTGATCTT+TGG | - | Chr5:24940850-24940869 | MsG0580025839.01.T01:CDS | 30.0% | |
| AGGTACTTGAATCTTTCTTA+TGG | - | Chr5:24941305-24941324 | MsG0580025839.01.T01:CDS | 30.0% | |
| AGGTTGAGTAATAATTGATG+TGG | + | Chr5:24940022-24940041 | None:intergenic | 30.0% | |
| AGTAAGCTATGGAATTTACA+AGG | - | Chr5:24940717-24940736 | MsG0580025839.01.T01:CDS | 30.0% | |
| ATGTAGTTGGCAAGAAAAAA+GGG | - | Chr5:24941534-24941553 | MsG0580025839.01.T01:CDS | 30.0% | |
| ATTACTCAACCTCAAGTTTA+TGG | - | Chr5:24940030-24940049 | MsG0580025839.01.T01:CDS | 30.0% | |
| ATTGTAAATTGAGAAAGGTG+TGG | + | Chr5:24942079-24942098 | None:intergenic | 30.0% | |
| CAGCAATTGTAAATTGAGAA+AGG | + | Chr5:24942084-24942103 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 24939547 | 24942849 | 24939547 | ID=MsG0580025839.01;Name=MsG0580025839.01 |
| Chr5 | mRNA | 24939547 | 24942849 | 24939547 | ID=MsG0580025839.01.T01;Parent=MsG0580025839.01;Name=MsG0580025839.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|1100 |
| Chr5 | exon | 24939547 | 24942849 | 24939547 | ID=MsG0580025839.01.T01:exon:4985;Parent=MsG0580025839.01.T01 |
| Chr5 | CDS | 24939547 | 24942849 | 24939547 | ID=MsG0580025839.01.T01:cds;Parent=MsG0580025839.01.T01 |
| Gene Sequence |
>MsG0580025839.01.T01 ATGGCTGAGGCAGTCATTGAAGTTGTCCTTGATAATTTGAGCACACTCATTCAGAAGGAGCTTGGCCTATTTCTAGGTGTTGATCGTGAATTGAGAAGTCTTTCTAGCTTGCTAGCTACAATCAAGGCTACCCTTGAAGATGCTGAGGAGAAGCAATTCTCAAACAGAGCTATCAAGGATTGGCTGCTTAAGCTCAAAGATGCTGCTCACATCCTCGATGACATCTTGGACGAGTGTGCCACCAAAGCACTAGAACTGGAGTACAAAGGATTCAAGTATGGACCATCACACAAGGTACAAAGCTCTTGCTTATCCTCTTTAAATCCTAAGAATGTTGCTTTCCGTTACAAAATTGCTAAGAAAATGAAGAGGATAAGGGAGAGATTGGATGGAATAGCTGAAGAAAGGAGTAAGTTTCATTTGACTGAGATAGTCAGAGAAAGGAGATGCGAAGTCCTTGACTGGCGACAAACCACATCAATTATTACTCAACCTCAAGTTTATGGAAGAGATGAAGATAAGAGTAAAATTGTAGACTTTTTGGTTGATGATGCTTCTAGTTTTGAGGATTTATCAGTTTATCCAATTGTCGGTTTAGGTGGACTTGGGAAAACAACACTTGCCCAACTTGTGTTCAATCATGAGAAGGTAGTCAACCACTTTGAGCGAAGAATTTGGGTATGCGTTTCAGAAGATTTCAGTTTGAAGAGAATGACAAAAGCCATCATAGAAGCTGAATCTGGCCATGCTTCCGAGGAATTAGATCTAGAACCGCTGCAAAGAAAACTGTTGGATTTGCTACGAAGAAAAAGATACTTGCTGGTGTTGGATGATGTATGGGACGATGGGCAAGAGAATTGGGAGAGATTGAGATCTGTATTGGATTATGGAGGCAAGGGTGCTTCGGTTTTGGTAACCACTCGTCTTCCCAAGGTTGCAGCAATCATGGGAACGATGCCCTCTCATGATTTATCAATGCTATCTGACACTGATTGTTGGGAATTGTTTAAACAACGGGCATTTGGACCAACTGAGGCTGAACGGTCAGATCTTGCAGTGATAGGAAAGGAGATAGTAAAAAAGTGTAGGGGAGTCCCTCTCGCAGCGAAAGCACTGGGAAGTTTATTGCGGTTTAAACGAGAAGAAATGGAATGGCTCTATGTCAAGGAAAGTAAGCTATGGAATTTACAAGGTGAGAATTCTGTCATGCCTGCATTGAGATTAAGTTACTTGAATTTGCCGGTAAAATTGAGACAGTGTTTTGCCTTCTGCGCACTATTTCCCAAAGATGAAAGAATAAGCAAGCAGTTACTAATTGATCTTTGGATGGCTAATGGATTTATTCCATCCAATGGAATGTTGGAAGCAGAGGATATTGGTAATGAGGCGTGGAATGAGTTATACTGTAGATCATTTTTTCAAGATACTCAGACAGATGAATTTGGACAAATTGCATATTTCTATATGCATGATCTTGTTCATGATCTTGCTCAATCTATCACAGAAGAGGTCTGTCACATTACAAATGATAGTGGCATACCTAGTATGTCTGAAAGAATCCGCCATCTCTCAATTTGTAGGAGCGACTTTTTTGGGAAAGTTGGCTCAATCAGGTTACATAATGTTGAAGCGTTAAAGACATGTATACATAATGTTGACCAACTTTCACCTCATGTATTGAAATGTTATAATTTACGAGTGCTTGACTTTAATTGGGGAAAAAAGTTGCCGTCTTCAATTGGTCGTTTAAAATATCTAAGGTACTTGAATCTTTCTTATGGAGAATTCAAAACTCTTCCAGAATCCTTATGTGAGTTGTGGAATTTGCAGATTTTAAAGTTAGACTATTGTCAAAATCTCCAAAAGTTGCCTAATAGTCTAGTACACTTGAAAGCTCTACAACATCTATATTTGAGAAGCTGCTATTCACTATCAAGTTTGCCCCCACATGTAAGGATGTTGGCATCCTTGAAAACTTTAACATTGTATGTAGTTGGCAAGAAAAAAGGGTTTCTTTTAGCAGAGTTAGGACAGATGATCCTTCAAGGAGACCTTCACATTGAGCACTTGGAGAGAGTTAAAAGTGTAATGAATGCCGCAGAAGCTAATATGTCAAGCAAGTGCGTGGACAAGTTGGAATTGTCATGGGATAGAAATGAAGAGTCACAATTACAGAAAAATGTTGAGGAGATTCTTGAAGTGCTTCAACCACAAACTCAGCAACTTCGAAGCCTGGGTGTTCGAGGATATACCGGTTCCTGTTTCCCAGAATGGATGTCTAGTCCTACTCTAAAATATTTAACTAGTTTACAACTCGTGGATTGCAAAAGTTGTTTACACCTTCCGCATTTGGGGAAGCTACCATCCCTAAAGAGTCTAACAGTATCTAACATGAGTCATGTAAAATACTTAGACGAGGAGTCATGCGATGATGGAGTTGCAGGAGGTTTTATATGTCTAGAAAAACTGGTTCTAGTGGAGCTGCCAAACCTGATAATTTTATCAAGGGATGATAGAGAAAACATGCTCCCACACCTTTCTCAATTTACAATTGCTGAATGTCCTAAATTGTTGGGCTTGCCTTGCCTTCCATCTCTCATTGATATGATTATATTCGGAAAATGCAACACGGGTTTACTAAGTTCAATTCAAAAACTCGTCAGTCTTGAATCCCTTATGTTCATTGGCAATGAAGAACTAACTTGCTTTCCAGATGGGATGCTAAGAAACCTCAATTCTCTAAAAAAAATTGACATTCGTAGGCTTTCCAGACTTGAAAGCTTCCCAACTGAAATCATTAACCTTAGTGCTGTCCAAGAAATATGTATTACTGCATGTGAAAACCTCAAGTCATTGACAGATGAAGTATTACAATGGCTGCACTCTCTTAAGAGATTGAGTATTGTGAAGTACCAAAAGTTCAACCTATCTGAAAATTTTCAATACCTCACTTGTCTTGAGAAATTGGTGATCCAAAGTTGCTCAGAAATAGAAGTTTTGCATGAGAGTTTACAGCATATGACTTCCCTTCAATCACTGACATTGTGTGATCTTCCAAATCTTGCATCCATACCTGACTGGTTAGGAAACCTAAGTTTGCTTCAAGAATTAAATATTTCTCAGTGTCCAAAGCTGACGTGCCTTCCCATGAGCATTCAATGCCTTACTGGTCTGAAACGTTTGAGTATTTACAGTTGCAGTGAGTTAGAGAAACGGTGTAAAGAGAAAACAGGTGAGGATTGGCCAAAAATAGCTCACATTCAATGCATTGCAGTAGAAAACATCAGAATGTTCTACGGATAA |
| Protein sequence |
>MsG0580025839.01.T01 MAEAVIEVVLDNLSTLIQKELGLFLGVDRELRSLSSLLATIKATLEDAEEKQFSNRAIKDWLLKLKDAAHILDDILDECATKALELEYKGFKYGPSHKVQSSCLSSLNPKNVAFRYKIAKKMKRIRERLDGIAEERSKFHLTEIVRERRCEVLDWRQTTSIITQPQVYGRDEDKSKIVDFLVDDASSFEDLSVYPIVGLGGLGKTTLAQLVFNHEKVVNHFERRIWVCVSEDFSLKRMTKAIIEAESGHASEELDLEPLQRKLLDLLRRKRYLLVLDDVWDDGQENWERLRSVLDYGGKGASVLVTTRLPKVAAIMGTMPSHDLSMLSDTDCWELFKQRAFGPTEAERSDLAVIGKEIVKKCRGVPLAAKALGSLLRFKREEMEWLYVKESKLWNLQGENSVMPALRLSYLNLPVKLRQCFAFCALFPKDERISKQLLIDLWMANGFIPSNGMLEAEDIGNEAWNELYCRSFFQDTQTDEFGQIAYFYMHDLVHDLAQSITEEVCHITNDSGIPSMSERIRHLSICRSDFFGKVGSIRLHNVEALKTCIHNVDQLSPHVLKCYNLRVLDFNWGKKLPSSIGRLKYLRYLNLSYGEFKTLPESLCELWNLQILKLDYCQNLQKLPNSLVHLKALQHLYLRSCYSLSSLPPHVRMLASLKTLTLYVVGKKKGFLLAELGQMILQGDLHIEHLERVKSVMNAAEANMSSKCVDKLELSWDRNEESQLQKNVEEILEVLQPQTQQLRSLGVRGYTGSCFPEWMSSPTLKYLTSLQLVDCKSCLHLPHLGKLPSLKSLTVSNMSHVKYLDEESCDDGVAGGFICLEKLVLVELPNLIILSRDDRENMLPHLSQFTIAECPKLLGLPCLPSLIDMIIFGKCNTGLLSSIQKLVSLESLMFIGNEELTCFPDGMLRNLNSLKKIDIRRLSRLESFPTEIINLSAVQEICITACENLKSLTDEVLQWLHSLKRLSIVKYQKFNLSENFQYLTCLEKLVIQSCSEIEVLHESLQHMTSLQSLTLCDLPNLASIPDWLGNLSLLQELNISQCPKLTCLPMSIQCLTGLKRLSIYSCSELEKRCKEKTGEDWPKIAHIQCIAVENIRMFYG* |
