Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026001.01.T01 | XP_024627564.1 | 97.561 | 123 | 3 | 0 | 55 | 177 | 143 | 265 | 4.69E-81 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026001.01.T01 | Q5XF11 | 53.247 | 154 | 62 | 6 | 32 | 176 | 168 | 320 | 3.97E-43 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026001.01.T01 | G7LB69 | 95.935 | 123 | 4 | 1 | 55 | 177 | 143 | 264 | 4.45e-77 | 240 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007596.01 | MsG0580026001.01 | -0.818959 | 1.492865e-52 | 1.133784e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026001.01.T01 | MTR_8g075190 | 95.935 | 123 | 4 | 1 | 55 | 177 | 143 | 264 | 1.13e-80 | 240 |
| MsG0580026001.01.T01 | MTR_3g117710 | 87.037 | 54 | 7 | 0 | 1 | 54 | 290 | 343 | 2.23e-24 | 99.4 |
| MsG0580026001.01.T01 | MTR_1g096140 | 61.111 | 72 | 27 | 1 | 1 | 71 | 271 | 342 | 9.33e-20 | 85.9 |
| MsG0580026001.01.T01 | MTR_4g075540 | 52.113 | 71 | 34 | 0 | 1 | 71 | 230 | 300 | 1.01e-18 | 82.8 |
| MsG0580026001.01.T01 | MTR_1g095970 | 58.333 | 72 | 29 | 1 | 1 | 71 | 269 | 340 | 2.24e-18 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026001.01.T01 | AT4G35930 | 53.247 | 154 | 63 | 5 | 32 | 176 | 168 | 321 | 8.18e-46 | 153 |
| MsG0580026001.01.T01 | AT4G35930 | 53.247 | 154 | 63 | 5 | 32 | 176 | 168 | 321 | 8.18e-46 | 153 |
| MsG0580026001.01.T01 | AT4G35930 | 53.247 | 154 | 62 | 6 | 32 | 176 | 168 | 320 | 4.05e-44 | 149 |
| MsG0580026001.01.T01 | AT1G22650 | 66.197 | 71 | 24 | 0 | 1 | 71 | 252 | 322 | 2.41e-23 | 96.3 |
| MsG0580026001.01.T01 | AT1G22650 | 66.197 | 71 | 24 | 0 | 1 | 71 | 252 | 322 | 2.56e-23 | 95.9 |
| MsG0580026001.01.T01 | AT1G72000 | 63.380 | 71 | 26 | 0 | 1 | 71 | 217 | 287 | 1.21e-22 | 94.4 |
| MsG0580026001.01.T01 | AT4G09510 | 63.380 | 71 | 26 | 0 | 1 | 71 | 275 | 345 | 2.90e-22 | 92.8 |
| MsG0580026001.01.T01 | AT4G09510 | 63.380 | 71 | 26 | 0 | 1 | 71 | 275 | 345 | 3.29e-22 | 93.2 |
| MsG0580026001.01.T01 | AT1G35580 | 74.074 | 54 | 14 | 0 | 1 | 54 | 267 | 320 | 2.12e-21 | 90.5 |
| MsG0580026001.01.T01 | AT1G35580 | 74.074 | 54 | 14 | 0 | 1 | 54 | 267 | 320 | 2.52e-21 | 90.5 |
| MsG0580026001.01.T01 | AT1G35580 | 74.074 | 54 | 14 | 0 | 1 | 54 | 267 | 320 | 2.52e-21 | 90.5 |
| MsG0580026001.01.T01 | AT4G34860 | 64.789 | 71 | 25 | 0 | 1 | 71 | 288 | 358 | 2.62e-21 | 90.5 |
| MsG0580026001.01.T01 | AT4G34860 | 64.789 | 71 | 25 | 0 | 1 | 71 | 288 | 358 | 2.62e-21 | 90.5 |
| MsG0580026001.01.T01 | AT4G34860 | 64.789 | 71 | 25 | 0 | 1 | 71 | 288 | 358 | 2.62e-21 | 90.5 |
Find 42 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCAAGAATTGTTCTTTA+TGG | 0.080038 | 5:-28946974 | MsG0580026001.01.T01:CDS |
| TTATGGTTTCAGGGTGTTTA+TGG | 0.155228 | 5:-28947009 | None:intergenic |
| GTCGCATCTCAGAAACTTTA+AGG | 0.207710 | 5:+28946250 | None:intergenic |
| TGCTGAAGGGAAAGAGTTTA+TGG | 0.259291 | 5:-28946917 | MsG0580026001.01.T01:CDS |
| AACTCTTTCCCTTCAGCATC+TGG | 0.317968 | 5:+28946922 | None:intergenic |
| ATCATATGAGAAGCCACTTT+TGG | 0.334217 | 5:-28946861 | MsG0580026001.01.T01:CDS |
| AACAATTCTTGAATTTCAAT+TGG | 0.347066 | 5:+28946982 | None:intergenic |
| ACTGATTTGCACTGAGGCTT+TGG | 0.358652 | 5:+28946162 | None:intergenic |
| ATCTCTAGCCGAGAGCGGTC+TGG | 0.359600 | 5:+28946478 | None:intergenic |
| GGCCCATGACAAGGTGCTTT+AGG | 0.389444 | 5:+28946288 | None:intergenic |
| GCTTTAGGTGTATGCGGGCT+TGG | 0.400069 | 5:+28946303 | None:intergenic |
| TCTCTAGCCGAGAGCGGTCT+GGG | 0.400881 | 5:+28946479 | None:intergenic |
| ATCTGGCTTAAGCAATTTCA+AGG | 0.441923 | 5:+28946939 | None:intergenic |
| GGCATTCGCTTCTCGTTGCA+TGG | 0.467088 | 5:-28946199 | MsG0580026001.01.T01:CDS |
| TTCCAGCAGGGGTGATGGGA+AGG | 0.471360 | 5:-28946337 | MsG0580026001.01.T01:intron |
| CTCCTCGTCCTTCTGAGCAT+TGG | 0.480952 | 5:-28946441 | MsG0580026001.01.T01:CDS |
| TACTTCAAACCAACCAAAAG+TGG | 0.484244 | 5:+28946848 | None:intergenic |
| GGGGTGATGGGAAGGGACTA+AGG | 0.492038 | 5:-28946329 | MsG0580026001.01.T01:CDS |
| TTTATTCCAGCAGGGGTGAT+GGG | 0.511432 | 5:-28946341 | MsG0580026001.01.T01:intron |
| ATATTACCCCAGACCGCTCT+CGG | 0.514078 | 5:-28946486 | MsG0580026001.01.T01:CDS |
| TAAAGTTTCTGAGATGCGAC+AGG | 0.519219 | 5:-28946247 | MsG0580026001.01.T01:CDS |
| AGAAACACTTACCACAGAAA+AGG | 0.519980 | 5:+28946418 | None:intergenic |
| AGAAAAGGCCAATGCTCAGA+AGG | 0.546721 | 5:+28946433 | None:intergenic |
| ACACCTAAAGCACCTTGTCA+TGG | 0.549616 | 5:-28946291 | MsG0580026001.01.T01:CDS |
| AAGGTGCTTTAGGTGTATGC+GGG | 0.553664 | 5:+28946298 | None:intergenic |
| CACCTAAAGCACCTTGTCAT+GGG | 0.571278 | 5:-28946290 | MsG0580026001.01.T01:CDS |
| TCCAGCAGGGGTGATGGGAA+GGG | 0.571362 | 5:-28946336 | MsG0580026001.01.T01:intron |
| TTGCTTAAGCCAGATGCTGA+AGG | 0.574006 | 5:-28946931 | MsG0580026001.01.T01:CDS |
| CTGTGAAGATGAACTATGCA+AGG | 0.576710 | 5:-28946118 | MsG0580026001.01.T01:CDS |
| TGCTTAAGCCAGATGCTGAA+GGG | 0.592809 | 5:-28946930 | MsG0580026001.01.T01:CDS |
| TTAAGGCGAGAAGGAGGTCG+AGG | 0.593863 | 5:+28946267 | None:intergenic |
| TGAGGCTTTGGTATAACTGA+TGG | 0.602425 | 5:+28946174 | None:intergenic |
| CAAGGTGCTTTAGGTGTATG+CGG | 0.606559 | 5:+28946297 | None:intergenic |
| TCAGAAACTTTAAGGCGAGA+AGG | 0.612804 | 5:+28946258 | None:intergenic |
| CTCTAGCCGAGAGCGGTCTG+GGG | 0.623421 | 5:+28946480 | None:intergenic |
| GGAGGTCGAGGCCCATGACA+AGG | 0.632998 | 5:+28946279 | None:intergenic |
| GGCCAATGCTCAGAAGGACG+AGG | 0.635262 | 5:+28946439 | None:intergenic |
| GCTTCTCATATGATAGCTCA+AGG | 0.639444 | 5:+28946870 | None:intergenic |
| GAAACTTTAAGGCGAGAAGG+AGG | 0.650069 | 5:+28946261 | None:intergenic |
| AAAGCCTCAGTGCAAATCAG+TGG | 0.651345 | 5:-28946160 | MsG0580026001.01.T01:CDS |
| GAAGCCACTGATTTGCACTG+AGG | 0.675121 | 5:+28946156 | None:intergenic |
| TTAACATCTCTAGCCGAGAG+CGG | 0.681988 | 5:+28946473 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAATTCTTGAATTTCAAT+TGG | + | Chr5:28946110-28946129 | None:intergenic | 20.0% |
| !! | AAGACATGATTTAAAAAACA+CGG | + | Chr5:28946714-28946733 | None:intergenic | 20.0% |
| !! | AATTCAAGAATTGTTCTTTA+TGG | - | Chr5:28946115-28946134 | MsG0580026001.01.T01:CDS | 20.0% |
| ! | AGACATGATTTAAAAAACAC+GGG | + | Chr5:28946713-28946732 | None:intergenic | 25.0% |
| ! | AGTATGCTTCATATTAACTT+TGG | - | Chr5:28946325-28946344 | MsG0580026001.01.T01:CDS | 25.0% |
| !!! | CTAACTTTTCTAGTGTTTTT+AGG | - | Chr5:28946550-28946569 | MsG0580026001.01.T01:intron | 25.0% |
| !!! | TTTTCTAATTCTGAGAAACA+TGG | + | Chr5:28946443-28946462 | None:intergenic | 25.0% |
| AGCATACTCAATATAGAATC+AGG | + | Chr5:28946313-28946332 | None:intergenic | 30.0% | |
| CCACTTCAATTCATCATAAA+CGG | + | Chr5:28946511-28946530 | None:intergenic | 30.0% | |
| CCTCAAATTGTGAAAGAAAA+TGG | + | Chr5:28946374-28946393 | None:intergenic | 30.0% | |
| ! | CCATTTTCTTTCACAATTTG+AGG | - | Chr5:28946371-28946390 | MsG0580026001.01.T01:intron | 30.0% |
| ! | CTATAATTTTATTCCAGCAG+GGG | - | Chr5:28946741-28946760 | MsG0580026001.01.T01:intron | 30.0% |
| ! | GCTATAATTTTATTCCAGCA+GGG | - | Chr5:28946740-28946759 | MsG0580026001.01.T01:intron | 30.0% |
| ! | TGCTATAATTTTATTCCAGC+AGG | - | Chr5:28946739-28946758 | MsG0580026001.01.T01:intron | 30.0% |
| !! | AACTGTTCTTTTCAAGGAAT+CGG | - | Chr5:28946869-28946888 | MsG0580026001.01.T01:CDS | 30.0% |
| !!! | GTTTTTAGGTTATTGTTGCA+AGG | - | Chr5:28946564-28946583 | MsG0580026001.01.T01:intron | 30.0% |
| AAACACGGGAAAGAGATAAT+CGG | + | Chr5:28946699-28946718 | None:intergenic | 35.0% | |
| AGAAACACTTACCACAGAAA+AGG | + | Chr5:28946674-28946693 | None:intergenic | 35.0% | |
| CATCATAAACGGATACTCAT+GGG | + | Chr5:28946500-28946519 | None:intergenic | 35.0% | |
| CCGTTTATGATGAATTGAAG+TGG | - | Chr5:28946508-28946527 | MsG0580026001.01.T01:CDS | 35.0% | |
| TACTTCAAACCAACCAAAAG+TGG | + | Chr5:28946244-28946263 | None:intergenic | 35.0% | |
| TCATCATAAACGGATACTCA+TGG | + | Chr5:28946501-28946520 | None:intergenic | 35.0% | |
| ! | ATCTGGCTTAAGCAATTTCA+AGG | + | Chr5:28946153-28946172 | None:intergenic | 35.0% |
| ! | GGTTACAACTGTTCTTTTCA+AGG | - | Chr5:28946863-28946882 | MsG0580026001.01.T01:CDS | 35.0% |
| !! | ATCATATGAGAAGCCACTTT+TGG | - | Chr5:28946228-28946247 | MsG0580026001.01.T01:CDS | 35.0% |
| AAACAGCTCTCAGTTGATCA+TGG | + | Chr5:28946419-28946438 | None:intergenic | 40.0% | |
| CTGTGAAGATGAACTATGCA+AGG | - | Chr5:28946971-28946990 | MsG0580026001.01.T01:CDS | 40.0% | |
| GCTCAGAATAAACTTCTCTG+AGG | - | Chr5:28946999-28947018 | MsG0580026001.01.T01:CDS | 40.0% | |
| GCTTCTCATATGATAGCTCA+AGG | + | Chr5:28946222-28946241 | None:intergenic | 40.0% | |
| GTCGCATCTCAGAAACTTTA+AGG | + | Chr5:28946842-28946861 | None:intergenic | 40.0% | |
| TCATGGGCATATAAACAGCA+GGG | + | Chr5:28946484-28946503 | None:intergenic | 40.0% | |
| TGCTGAAGGGAAAGAGTTTA+TGG | - | Chr5:28946172-28946191 | MsG0580026001.01.T01:CDS | 40.0% | |
| ! | TGAGGCTTTGGTATAACTGA+TGG | + | Chr5:28946918-28946937 | None:intergenic | 40.0% |
| !! | TAAAGTTTCTGAGATGCGAC+AGG | - | Chr5:28946842-28946861 | MsG0580026001.01.T01:intron | 40.0% |
| !! | TCAGAAACTTTAAGGCGAGA+AGG | + | Chr5:28946834-28946853 | None:intergenic | 40.0% |
| !!! | TATGAGAAGCCACTTTTGGT+TGG | - | Chr5:28946232-28946251 | MsG0580026001.01.T01:CDS | 40.0% |
| AAAGCCTCAGTGCAAATCAG+TGG | - | Chr5:28946929-28946948 | MsG0580026001.01.T01:CDS | 45.0% | |
| AACTCTTTCCCTTCAGCATC+TGG | + | Chr5:28946170-28946189 | None:intergenic | 45.0% | |
| AGAAAAGGCCAATGCTCAGA+AGG | + | Chr5:28946659-28946678 | None:intergenic | 45.0% | |
| CTCATGGGCATATAAACAGC+AGG | + | Chr5:28946485-28946504 | None:intergenic | 45.0% | |
| TGCTTAAGCCAGATGCTGAA+GGG | - | Chr5:28946159-28946178 | MsG0580026001.01.T01:CDS | 45.0% | |
| TTAACATCTCTAGCCGAGAG+CGG | + | Chr5:28946619-28946638 | None:intergenic | 45.0% | |
| TTGCTTAAGCCAGATGCTGA+AGG | - | Chr5:28946158-28946177 | MsG0580026001.01.T01:CDS | 45.0% | |
| TTTATTCCAGCAGGGGTGAT+GGG | - | Chr5:28946748-28946767 | MsG0580026001.01.T01:intron | 45.0% | |
| ! | AAGGTGCTTTAGGTGTATGC+GGG | + | Chr5:28946794-28946813 | None:intergenic | 45.0% |
| ! | ACACCTAAAGCACCTTGTCA+TGG | - | Chr5:28946798-28946817 | MsG0580026001.01.T01:intron | 45.0% |
| ! | CAAGGTGCTTTAGGTGTATG+CGG | + | Chr5:28946795-28946814 | None:intergenic | 45.0% |
| ! | CACCTAAAGCACCTTGTCAT+GGG | - | Chr5:28946799-28946818 | MsG0580026001.01.T01:intron | 45.0% |
| ! | TTTTATTCCAGCAGGGGTGA+TGG | - | Chr5:28946747-28946766 | MsG0580026001.01.T01:intron | 45.0% |
| !! | ACTGATTTGCACTGAGGCTT+TGG | + | Chr5:28946930-28946949 | None:intergenic | 45.0% |
| !! | GAAACTTTAAGGCGAGAAGG+AGG | + | Chr5:28946831-28946850 | None:intergenic | 45.0% |
| ATATTACCCCAGACCGCTCT+CGG | - | Chr5:28946603-28946622 | MsG0580026001.01.T01:intron | 50.0% | |
| CTCTCAGTTGATCATGGTGC+AGG | + | Chr5:28946413-28946432 | None:intergenic | 50.0% | |
| !! | GAAGCCACTGATTTGCACTG+AGG | + | Chr5:28946936-28946955 | None:intergenic | 50.0% |
| !!! | CTGAGCATTGGCCTTTTCTG+TGG | - | Chr5:28946660-28946679 | MsG0580026001.01.T01:intron | 50.0% |
| CTCCTCGTCCTTCTGAGCAT+TGG | - | Chr5:28946648-28946667 | MsG0580026001.01.T01:intron | 55.0% | |
| GGCATTCGCTTCTCGTTGCA+TGG | - | Chr5:28946890-28946909 | MsG0580026001.01.T01:CDS | 55.0% | |
| TTAAGGCGAGAAGGAGGTCG+AGG | + | Chr5:28946825-28946844 | None:intergenic | 55.0% | |
| ! | GCTTTAGGTGTATGCGGGCT+TGG | + | Chr5:28946789-28946808 | None:intergenic | 55.0% |
| ! | GGCCCATGACAAGGTGCTTT+AGG | + | Chr5:28946804-28946823 | None:intergenic | 55.0% |
| ATCTCTAGCCGAGAGCGGTC+TGG | + | Chr5:28946614-28946633 | None:intergenic | 60.0% | |
| GGCCAATGCTCAGAAGGACG+AGG | + | Chr5:28946653-28946672 | None:intergenic | 60.0% | |
| TCTCTAGCCGAGAGCGGTCT+GGG | + | Chr5:28946613-28946632 | None:intergenic | 60.0% | |
| !! | GGGGTGATGGGAAGGGACTA+AGG | - | Chr5:28946760-28946779 | MsG0580026001.01.T01:intron | 60.0% |
| !! | TCCAGCAGGGGTGATGGGAA+GGG | - | Chr5:28946753-28946772 | MsG0580026001.01.T01:intron | 60.0% |
| !! | TTCCAGCAGGGGTGATGGGA+AGG | - | Chr5:28946752-28946771 | MsG0580026001.01.T01:intron | 60.0% |
| CTCTAGCCGAGAGCGGTCTG+GGG | + | Chr5:28946612-28946631 | None:intergenic | 65.0% | |
| GGAGGTCGAGGCCCATGACA+AGG | + | Chr5:28946813-28946832 | None:intergenic | 65.0% | |
| TCCCTTCCCATCACCCCTGC+TGG | + | Chr5:28946757-28946776 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 28946092 | 28947019 | 28946092 | ID=MsG0580026001.01;Name=MsG0580026001.01 |
| Chr5 | mRNA | 28946092 | 28947019 | 28946092 | ID=MsG0580026001.01.T01;Parent=MsG0580026001.01;Name=MsG0580026001.01.T01;_AED=0.24;_eAED=0.24;_QI=0|0|0|0.66|0.5|0.66|3|0|177 |
| Chr5 | exon | 28946858 | 28947019 | 28946858 | ID=MsG0580026001.01.T01:exon:33366;Parent=MsG0580026001.01.T01 |
| Chr5 | exon | 28946430 | 28946539 | 28946430 | ID=MsG0580026001.01.T01:exon:33365;Parent=MsG0580026001.01.T01 |
| Chr5 | exon | 28946092 | 28946353 | 28946092 | ID=MsG0580026001.01.T01:exon:33364;Parent=MsG0580026001.01.T01 |
| Chr5 | CDS | 28946858 | 28947019 | 28946858 | ID=MsG0580026001.01.T01:cds;Parent=MsG0580026001.01.T01 |
| Chr5 | CDS | 28946430 | 28946539 | 28946430 | ID=MsG0580026001.01.T01:cds;Parent=MsG0580026001.01.T01 |
| Chr5 | CDS | 28946092 | 28946353 | 28946092 | ID=MsG0580026001.01.T01:cds;Parent=MsG0580026001.01.T01 |
| Gene Sequence |
| Protein sequence |
