Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026090.01.T01 | GAU51297.1 | 67.925 | 212 | 27 | 7 | 2 | 178 | 187 | 392 | 2.78E-70 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026090.01.T01 | A0A2Z6PVP9 | 67.925 | 212 | 27 | 7 | 2 | 178 | 187 | 392 | 1.33e-70 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580026090.01 | MsG0580030006.01 | 0.818750 | 1.665143e-52 | 1.257188e-49 |
| MsG0580026090.01 | MsG0680031702.01 | 0.822193 | 2.699630e-53 | 2.243732e-50 |
| MsG0580026090.01 | MsG0780041262.01 | 0.818175 | 2.248464e-52 | 1.670884e-49 |
| MsG0580026090.01 | MsG0880044043.01 | 0.818860 | 1.572597e-52 | 1.190998e-49 |
| MsG0580026090.01 | MsG0880047432.01 | 0.811759 | 5.962892e-51 | 3.722389e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026090.01.T01 | MTR_5g039600 | 73.558 | 208 | 16 | 3 | 3 | 174 | 189 | 393 | 4.36e-73 | 226 |
| MsG0580026090.01.T01 | MTR_4g108370 | 41.259 | 143 | 67 | 6 | 60 | 192 | 301 | 436 | 8.57e-18 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 63 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCCTTCTGTTGGAAATAA+TGG | 0.210892 | 5:-30596049 | MsG0580026090.01.T01:CDS |
| AGGAGGAGGTGGTGGTATTT+TGG | 0.273038 | 5:-30595909 | MsG0580026090.01.T01:CDS |
| GTAATGGAAATGATCCAATT+TGG | 0.290902 | 5:-30596075 | MsG0580026090.01.T01:CDS |
| CCTGAACCTAACTGACTTCC+AGG | 0.299306 | 5:+30595950 | None:intergenic |
| ACAGAAGGAATTGCCCAAAT+TGG | 0.300472 | 5:+30596061 | None:intergenic |
| CTTCAAATACGGCAGCGTTT+TGG | 0.303402 | 5:-30596141 | MsG0580026090.01.T01:CDS |
| AACCAAGGTGTTAATGTTAA+TGG | 0.358090 | 5:-30596100 | MsG0580026090.01.T01:CDS |
| CTTCCAGGCATAAGATTCAT+TGG | 0.360056 | 5:+30595965 | None:intergenic |
| CTTATGCCTGGAAGTCAGTT+AGG | 0.381627 | 5:-30595956 | MsG0580026090.01.T01:CDS |
| AAGCATACTCAGATTGCTTT+CGG | 0.383994 | 5:+30595886 | None:intergenic |
| GGTGGAGGAGGAGGAGGTGG+TGG | 0.394715 | 5:-30595917 | MsG0580026090.01.T01:CDS |
| CAGCATCACGGCGGAGGTGG+AGG | 0.398279 | 5:-30595806 | MsG0580026090.01.T01:CDS |
| GGAAATAATGGTTTATATAG+AGG | 0.402512 | 5:-30596037 | MsG0580026090.01.T01:CDS |
| TACAAGCAAAACAAGAGCTT+CGG | 0.403614 | 5:-30596329 | MsG0580026090.01.T01:CDS |
| TCAGGAATCGTCGGCGGTGG+AGG | 0.405826 | 5:-30595932 | MsG0580026090.01.T01:CDS |
| GGCGGTGGAGGAGGAGGAGG+TGG | 0.408059 | 5:-30595920 | MsG0580026090.01.T01:CDS |
| TAATGGAAATGATCCAATTT+GGG | 0.409142 | 5:-30596074 | MsG0580026090.01.T01:CDS |
| CACGGCGGAGGTGGAGGAGG+AGG | 0.417922 | 5:-30595800 | MsG0580026090.01.T01:CDS |
| CATCACGGCGGAGGTGGAGG+AGG | 0.423459 | 5:-30595803 | MsG0580026090.01.T01:CDS |
| TTTGGATGGTAACTGGTAAT+GGG | 0.423705 | 5:-30596123 | MsG0580026090.01.T01:CDS |
| TTCAAGCCATGCTTCAAATA+CGG | 0.431597 | 5:-30596152 | MsG0580026090.01.T01:CDS |
| AACATTGCTGATAAAGAAGA+AGG | 0.434505 | 5:+30596381 | None:intergenic |
| AAGCAATCTGAGTATGCTTT+CGG | 0.438504 | 5:-30595882 | MsG0580026090.01.T01:CDS |
| ATCGTCGGCGGTGGAGGAGG+AGG | 0.438624 | 5:-30595926 | MsG0580026090.01.T01:CDS |
| CTCCGCCACCAAGGCGGTGG+CGG | 0.438645 | 5:+30596296 | None:intergenic |
| GGAATCGTCGGCGGTGGAGG+AGG | 0.439995 | 5:-30595929 | MsG0580026090.01.T01:CDS |
| CCTGGAAGTCAGTTAGGTTC+AGG | 0.442518 | 5:-30595950 | MsG0580026090.01.T01:CDS |
| ATTTGGGCAATTCCTTCTGT+TGG | 0.444072 | 5:-30596058 | MsG0580026090.01.T01:CDS |
| GGAGCTATGTCATCTCCTAA+TGG | 0.444498 | 5:-30596016 | MsG0580026090.01.T01:CDS |
| GGACAGCATCACGGCGGAGG+TGG | 0.445597 | 5:-30595809 | MsG0580026090.01.T01:CDS |
| CGCCACCGCCTTGGTGGCGG+AGG | 0.455969 | 5:-30596295 | MsG0580026090.01.T01:CDS |
| ACAAGCAAAACAAGAGCTTC+GGG | 0.465254 | 5:-30596328 | MsG0580026090.01.T01:CDS |
| GTTAATGTTAATGGTGGTAA+TGG | 0.467651 | 5:-30596091 | MsG0580026090.01.T01:CDS |
| GGTGGAGGAGGAGGAGATGA+TGG | 0.469014 | 5:-30595791 | MsG0580026090.01.T01:CDS |
| CACCATTAACATTAACACCT+TGG | 0.473185 | 5:+30596098 | None:intergenic |
| GTTAGTCGAAATCTGCCTAT+AGG | 0.476356 | 5:+30595850 | None:intergenic |
| GTCGGCGGTGGAGGAGGAGG+AGG | 0.480664 | 5:-30595923 | MsG0580026090.01.T01:CDS |
| TTGTAGCTGATGATTCTAGA+AGG | 0.490333 | 5:-30596431 | MsG0580026090.01.T01:CDS |
| TTCATGAAATGAAATCCATT+AGG | 0.492064 | 5:+30596001 | None:intergenic |
| CACCGCCACCGCCTTGGTGG+CGG | 0.495885 | 5:-30596298 | MsG0580026090.01.T01:CDS |
| AGAAAACACCGCCACCGCCT+TGG | 0.502644 | 5:-30596304 | MsG0580026090.01.T01:CDS |
| GGTTCAGGAATCGTCGGCGG+TGG | 0.509951 | 5:-30595935 | MsG0580026090.01.T01:CDS |
| GTATTTGAAGCATGGCTTGA+AGG | 0.510350 | 5:+30596154 | None:intergenic |
| TAACGGTATGTCAGATTCGT+CGG | 0.514126 | 5:-30595831 | MsG0580026090.01.T01:CDS |
| ATCCTCCGCCACCAAGGCGG+TGG | 0.514521 | 5:+30596293 | None:intergenic |
| TCGGGACAGCATCACGGCGG+AGG | 0.519932 | 5:-30595812 | MsG0580026090.01.T01:CDS |
| TTTCGGCTATGAATGCCTAT+AGG | 0.521533 | 5:-30595865 | MsG0580026090.01.T01:CDS |
| AAACCATTATTTCCAACAGA+AGG | 0.534853 | 5:+30596046 | None:intergenic |
| TCTCCAATGAATCTTATGCC+TGG | 0.535265 | 5:-30595968 | MsG0580026090.01.T01:CDS |
| AAACACCGCCACCGCCTTGG+TGG | 0.535970 | 5:-30596301 | MsG0580026090.01.T01:CDS |
| AACGGTATGTCAGATTCGTC+GGG | 0.554182 | 5:-30595830 | MsG0580026090.01.T01:CDS |
| ACGCTGCCGTATTTGAAGCA+TGG | 0.572979 | 5:+30596146 | None:intergenic |
| TCAAGCAGTCAGCATTCCTA+AGG | 0.577498 | 5:-30595761 | None:intergenic |
| CAGTTAGGTTCAGGAATCGT+CGG | 0.577894 | 5:-30595941 | MsG0580026090.01.T01:CDS |
| CAAGGTGTTAATGTTAATGG+TGG | 0.607105 | 5:-30596097 | MsG0580026090.01.T01:CDS |
| GAGTAGTAACTCTACCATGT+TGG | 0.612610 | 5:-30596409 | MsG0580026090.01.T01:CDS |
| TATAGGCAGATTTCGACTAA+CGG | 0.622768 | 5:-30595848 | MsG0580026090.01.T01:CDS |
| GATTCGTCGGGACAGCATCA+CGG | 0.641317 | 5:-30595818 | MsG0580026090.01.T01:CDS |
| ATTCAAATCCTCCGCCACCA+AGG | 0.641339 | 5:+30596287 | None:intergenic |
| GTAACTGGTAATGGGAACCA+AGG | 0.670023 | 5:-30596115 | MsG0580026090.01.T01:CDS |
| TTAGGTTCAGGAATCGTCGG+CGG | 0.697792 | 5:-30595938 | MsG0580026090.01.T01:CDS |
| CAAATCCTCCGCCACCAAGG+CGG | 0.701522 | 5:+30596290 | None:intergenic |
| TCGTCGGGACAGCATCACGG+CGG | 0.801947 | 5:-30595815 | MsG0580026090.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATTATTATCACAAAATA+TGG | - | Chr5:30595989-30596008 | MsG0580026090.01.T01:CDS | 15.0% |
| !! | GAATTATTATCACAAAATAT+GGG | - | Chr5:30595990-30596009 | MsG0580026090.01.T01:CDS | 15.0% |
| !!! | ATTTGTTACAATCAAATGTA+GGG | - | Chr5:30596018-30596037 | MsG0580026090.01.T01:CDS | 20.0% |
| !!! | TATTTGTTACAATCAAATGT+AGG | - | Chr5:30596017-30596036 | MsG0580026090.01.T01:CDS | 20.0% |
| ! | GGAAATAATGGTTTATATAG+AGG | - | Chr5:30596164-30596183 | MsG0580026090.01.T01:CDS | 25.0% |
| ! | TAATGGAAATGATCCAATTT+GGG | - | Chr5:30596127-30596146 | MsG0580026090.01.T01:CDS | 25.0% |
| ! | TTCATGAAATGAAATCCATT+AGG | + | Chr5:30596203-30596222 | None:intergenic | 25.0% |
| AAACCATTATTTCCAACAGA+AGG | + | Chr5:30596158-30596177 | None:intergenic | 30.0% | |
| AACATTGCTGATAAAGAAGA+AGG | + | Chr5:30595823-30595842 | None:intergenic | 30.0% | |
| ATTCCTTCTGTTGGAAATAA+TGG | - | Chr5:30596152-30596171 | MsG0580026090.01.T01:CDS | 30.0% | |
| GTTAATGTTAATGGTGGTAA+TGG | - | Chr5:30596110-30596129 | MsG0580026090.01.T01:CDS | 30.0% | |
| ! | AACCAAGGTGTTAATGTTAA+TGG | - | Chr5:30596101-30596120 | MsG0580026090.01.T01:CDS | 30.0% |
| ! | GTAATGGAAATGATCCAATT+TGG | - | Chr5:30596126-30596145 | MsG0580026090.01.T01:CDS | 30.0% |
| AAGCAATCTGAGTATGCTTT+CGG | - | Chr5:30596319-30596338 | MsG0580026090.01.T01:CDS | 35.0% | |
| AAGCATACTCAGATTGCTTT+CGG | + | Chr5:30596318-30596337 | None:intergenic | 35.0% | |
| CACCATTAACATTAACACCT+TGG | + | Chr5:30596106-30596125 | None:intergenic | 35.0% | |
| TACAAGCAAAACAAGAGCTT+CGG | - | Chr5:30595872-30595891 | MsG0580026090.01.T01:CDS | 35.0% | |
| TATAGGCAGATTTCGACTAA+CGG | - | Chr5:30596353-30596372 | MsG0580026090.01.T01:CDS | 35.0% | |
| TTCAAGCCATGCTTCAAATA+CGG | - | Chr5:30596049-30596068 | MsG0580026090.01.T01:CDS | 35.0% | |
| TTGTAGCTGATGATTCTAGA+AGG | - | Chr5:30595770-30595789 | MsG0580026090.01.T01:CDS | 35.0% | |
| TTGTTAGGTTCAGATTCAGA+TGG | - | Chr5:30595936-30595955 | MsG0580026090.01.T01:CDS | 35.0% | |
| TTTGGATGGTAACTGGTAAT+GGG | - | Chr5:30596078-30596097 | MsG0580026090.01.T01:CDS | 35.0% | |
| ! | AGAAGAAGGATTTTCCAACA+TGG | + | Chr5:30595809-30595828 | None:intergenic | 35.0% |
| ! | CAAGGTGTTAATGTTAATGG+TGG | - | Chr5:30596104-30596123 | MsG0580026090.01.T01:CDS | 35.0% |
| ! | TTTTGGATGGTAACTGGTAA+TGG | - | Chr5:30596077-30596096 | MsG0580026090.01.T01:CDS | 35.0% |
| ACAAGCAAAACAAGAGCTTC+GGG | - | Chr5:30595873-30595892 | MsG0580026090.01.T01:CDS | 40.0% | |
| ACAGAAGGAATTGCCCAAAT+TGG | + | Chr5:30596143-30596162 | None:intergenic | 40.0% | |
| ATTTGGGCAATTCCTTCTGT+TGG | - | Chr5:30596143-30596162 | MsG0580026090.01.T01:CDS | 40.0% | |
| CTTCCAGGCATAAGATTCAT+TGG | + | Chr5:30596239-30596258 | None:intergenic | 40.0% | |
| GAAGCATAGGGAGAAAAAGA+AGG | - | Chr5:30595958-30595977 | MsG0580026090.01.T01:CDS | 40.0% | |
| GAGTAGTAACTCTACCATGT+TGG | - | Chr5:30595792-30595811 | MsG0580026090.01.T01:CDS | 40.0% | |
| GTTAGTCGAAATCTGCCTAT+AGG | + | Chr5:30596354-30596373 | None:intergenic | 40.0% | |
| TAACGGTATGTCAGATTCGT+CGG | - | Chr5:30596370-30596389 | MsG0580026090.01.T01:CDS | 40.0% | |
| TCTCCAATGAATCTTATGCC+TGG | - | Chr5:30596233-30596252 | MsG0580026090.01.T01:intron | 40.0% | |
| TTTCGGCTATGAATGCCTAT+AGG | - | Chr5:30596336-30596355 | MsG0580026090.01.T01:CDS | 40.0% | |
| ! | CAGATTCAGATGGAAGCATA+GGG | - | Chr5:30595946-30595965 | MsG0580026090.01.T01:CDS | 40.0% |
| ! | GTATTTGAAGCATGGCTTGA+AGG | + | Chr5:30596050-30596069 | None:intergenic | 40.0% |
| ! | TCAGATTCAGATGGAAGCAT+AGG | - | Chr5:30595945-30595964 | MsG0580026090.01.T01:CDS | 40.0% |
| AACGGTATGTCAGATTCGTC+GGG | - | Chr5:30596371-30596390 | MsG0580026090.01.T01:CDS | 45.0% | |
| CAGTTAGGTTCAGGAATCGT+CGG | - | Chr5:30596260-30596279 | MsG0580026090.01.T01:intron | 45.0% | |
| CTTATGCCTGGAAGTCAGTT+AGG | - | Chr5:30596245-30596264 | MsG0580026090.01.T01:intron | 45.0% | |
| GGAGCTATGTCATCTCCTAA+TGG | - | Chr5:30596185-30596204 | MsG0580026090.01.T01:intron | 45.0% | |
| GGCGGAGGATTTGAATTGTT+AGG | - | Chr5:30595921-30595940 | MsG0580026090.01.T01:CDS | 45.0% | |
| ! | CTTCAAATACGGCAGCGTTT+TGG | - | Chr5:30596060-30596079 | MsG0580026090.01.T01:CDS | 45.0% |
| !! | AAATACGGCAGCGTTTTGGA+TGG | - | Chr5:30596064-30596083 | MsG0580026090.01.T01:CDS | 45.0% |
| !! | GTAACTGGTAATGGGAACCA+AGG | - | Chr5:30596086-30596105 | MsG0580026090.01.T01:CDS | 45.0% |
| ATTCAAATCCTCCGCCACCA+AGG | + | Chr5:30595917-30595936 | None:intergenic | 50.0% | |
| CCTGGAAGTCAGTTAGGTTC+AGG | - | Chr5:30596251-30596270 | MsG0580026090.01.T01:intron | 50.0% | |
| TTAGGTTCAGGAATCGTCGG+CGG | - | Chr5:30596263-30596282 | MsG0580026090.01.T01:intron | 50.0% | |
| ! | ACGCTGCCGTATTTGAAGCA+TGG | + | Chr5:30596058-30596077 | None:intergenic | 50.0% |
| ! | CCTGAACCTAACTGACTTCC+AGG | + | Chr5:30596254-30596273 | None:intergenic | 50.0% |
| !! | AGGAGGAGGTGGTGGTATTT+TGG | - | Chr5:30596292-30596311 | MsG0580026090.01.T01:CDS | 50.0% |
| !! | GCAGCGTTTTGGATGGTAAC+TGG | - | Chr5:30596071-30596090 | MsG0580026090.01.T01:CDS | 50.0% |
| GATTCGTCGGGACAGCATCA+CGG | - | Chr5:30596383-30596402 | MsG0580026090.01.T01:CDS | 55.0% | |
| AGAAAACACCGCCACCGCCT+TGG | - | Chr5:30595897-30595916 | MsG0580026090.01.T01:CDS | 60.0% | |
| CAAATCCTCCGCCACCAAGG+CGG | + | Chr5:30595914-30595933 | None:intergenic | 60.0% | |
| GGTGGAGGAGGAGGAGATGA+TGG | - | Chr5:30596410-30596429 | MsG0580026090.01.T01:CDS | 60.0% | |
| AAACACCGCCACCGCCTTGG+TGG | - | Chr5:30595900-30595919 | MsG0580026090.01.T01:CDS | 65.0% | |
| GGTTCAGGAATCGTCGGCGG+TGG | - | Chr5:30596266-30596285 | MsG0580026090.01.T01:intron | 65.0% | |
| TCAGGAATCGTCGGCGGTGG+AGG | - | Chr5:30596269-30596288 | MsG0580026090.01.T01:intron | 65.0% | |
| TCGTCGGGACAGCATCACGG+CGG | - | Chr5:30596386-30596405 | MsG0580026090.01.T01:CDS | 65.0% | |
| ATCCTCCGCCACCAAGGCGG+TGG | + | Chr5:30595911-30595930 | None:intergenic | 70.0% | |
| ATCGTCGGCGGTGGAGGAGG+AGG | - | Chr5:30596275-30596294 | MsG0580026090.01.T01:intron | 70.0% | |
| CAGCATCACGGCGGAGGTGG+AGG | - | Chr5:30596395-30596414 | MsG0580026090.01.T01:CDS | 70.0% | |
| CATCACGGCGGAGGTGGAGG+AGG | - | Chr5:30596398-30596417 | MsG0580026090.01.T01:CDS | 70.0% | |
| GGAATCGTCGGCGGTGGAGG+AGG | - | Chr5:30596272-30596291 | MsG0580026090.01.T01:intron | 70.0% | |
| GGACAGCATCACGGCGGAGG+TGG | - | Chr5:30596392-30596411 | MsG0580026090.01.T01:CDS | 70.0% | |
| GGTGGAGGAGGAGGAGGTGG+TGG | - | Chr5:30596284-30596303 | MsG0580026090.01.T01:intron | 70.0% | |
| TCGGGACAGCATCACGGCGG+AGG | - | Chr5:30596389-30596408 | MsG0580026090.01.T01:CDS | 70.0% | |
| CACCGCCACCGCCTTGGTGG+CGG | - | Chr5:30595903-30595922 | MsG0580026090.01.T01:CDS | 75.0% | |
| CACGGCGGAGGTGGAGGAGG+AGG | - | Chr5:30596401-30596420 | MsG0580026090.01.T01:CDS | 75.0% | |
| CTCCGCCACCAAGGCGGTGG+CGG | + | Chr5:30595908-30595927 | None:intergenic | 75.0% | |
| GGCGGTGGAGGAGGAGGAGG+TGG | - | Chr5:30596281-30596300 | MsG0580026090.01.T01:intron | 75.0% | |
| GTCGGCGGTGGAGGAGGAGG+AGG | - | Chr5:30596278-30596297 | MsG0580026090.01.T01:intron | 75.0% | |
| !!! | CGCCACCGCCTTGGTGGCGG+AGG | - | Chr5:30595906-30595925 | MsG0580026090.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 30595763 | 30596460 | 30595763 | ID=MsG0580026090.01;Name=MsG0580026090.01 |
| Chr5 | mRNA | 30595763 | 30596460 | 30595763 | ID=MsG0580026090.01.T01;Parent=MsG0580026090.01;Name=MsG0580026090.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|198 |
| Chr5 | exon | 30596286 | 30596460 | 30596286 | ID=MsG0580026090.01.T01:exon:33858;Parent=MsG0580026090.01.T01 |
| Chr5 | exon | 30595763 | 30596184 | 30595763 | ID=MsG0580026090.01.T01:exon:33857;Parent=MsG0580026090.01.T01 |
| Chr5 | CDS | 30596286 | 30596460 | 30596286 | ID=MsG0580026090.01.T01:cds;Parent=MsG0580026090.01.T01 |
| Chr5 | CDS | 30595763 | 30596184 | 30595763 | ID=MsG0580026090.01.T01:cds;Parent=MsG0580026090.01.T01 |
| Gene Sequence |
| Protein sequence |
