AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026244.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026244.01.T01 MTR_5g042590 79.078 282 28 2 10 260 121 402 2.22e-143 431
MsG0580026244.01.T01 MTR_5g042590 97.727 88 2 0 263 350 491 578 2.63e-52 186
MsG0580026244.01.T01 MTR_4g056470 31.356 236 126 7 51 252 163 396 7.76e-23 100
MsG0580026244.01.T01 MTR_4g056430 30.638 235 126 8 51 251 125 356 1.92e-21 96.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026244.01.T01 AT1G69440 50.382 262 80 4 41 260 148 401 1.17e-72 243
MsG0580026244.01.T01 AT1G69440 76.471 68 16 0 263 330 490 557 5.30e-26 109
MsG0580026244.01.T01 AT2G27880 31.727 249 120 8 51 259 154 392 5.31e-21 95.1
MsG0580026244.01.T01 AT1G31290 30.415 217 121 9 44 234 298 510 1.37e-11 66.2
MsG0580026244.01.T01 AT1G31290 30.415 217 121 9 44 234 334 546 1.38e-11 66.2
MsG0580026244.01.T01 AT1G31290 30.415 217 121 9 44 234 260 472 1.38e-11 66.2

Find 79 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCCATCAACTTCTTTAA+AGG 0.105814 5:-33115093 None:intergenic
AGAACTCAAGCTTATCATTT+TGG 0.173580 5:-33115397 None:intergenic
TAGAGAAATCAAGCATAAAT+TGG 0.332646 5:+33115295 MsG0580026244.01.T01:CDS
GTTTGCTTCTGAATTTCCTT+TGG 0.334202 5:-33115062 None:intergenic
TTCTCTAGCAACATCCTTAG+AGG 0.337003 5:-33115279 None:intergenic
CCATCACCAAGCTTTAGTTT+TGG 0.355041 5:-33116651 None:intergenic
TGTTGTTCACGTTTCTCATA+AGG 0.358507 5:-33115458 None:intergenic
GTCGATTTGCTAGTTGGAAT+TGG 0.361272 5:-33115431 None:intergenic
GGCGCTTTGCCGGCATATGA+TGG 0.365628 5:+33115344 MsG0580026244.01.T01:CDS
TGCTCTCAATGTGGATTTCT+CGG 0.371590 5:+33115755 MsG0580026244.01.T01:CDS
GTTGCAAGGAGACCAGATTC+TGG 0.386600 5:+33115152 MsG0580026244.01.T01:CDS
CCTTCTTGTCCACCAGAATC+TGG 0.394128 5:-33115164 None:intergenic
AATTACTCCTCATCCCTCTA+AGG 0.396696 5:+33115265 MsG0580026244.01.T01:CDS
GTCATTCTATTCGAGTTCAA+TGG 0.405912 5:+33115652 MsG0580026244.01.T01:CDS
TCGATTTGCTAGTTGGAATT+GGG 0.409961 5:-33115430 None:intergenic
CCATCCTTTAAAGAAGTTGA+TGG 0.415148 5:+33115089 MsG0580026244.01.T01:CDS
TCATTCTATTCGAGTTCAAT+GGG 0.415940 5:+33115653 MsG0580026244.01.T01:CDS
CTGTGAAGGACAGAAGTTCC+TGG 0.421859 5:+33116140 MsG0580026244.01.T01:intron
GGTGATGTCGATTTGCTAGT+TGG 0.436112 5:-33115437 None:intergenic
CGGTGGAGGAGCTGTTGGAT+TGG 0.451562 5:+33115691 MsG0580026244.01.T01:CDS
ACTAAATACTTGAGCAAAGA+AGG 0.452270 5:+33115536 MsG0580026244.01.T01:CDS
GAGTTGTCTGCCTTTGAGAA+AGG 0.457326 5:-33115845 None:intergenic
TCTCATGACACCTTCGATGA+TGG 0.458848 5:-33116231 None:intergenic
TCGAGGCGTTTCTGCAAATA+CGG 0.479257 5:-33115810 None:intergenic
TACCCTCCAAAACTAAAGCT+TGG 0.483606 5:+33116645 MsG0580026244.01.T01:CDS
CCATCAACTTCTTTAAAGGA+TGG 0.485273 5:-33115089 None:intergenic
TTTCGAAGATTGACGGGAAG+GGG 0.485680 5:+33115510 MsG0580026244.01.T01:CDS
AGCTAACTGTGAAGAAAGAA+AGG 0.488444 5:+33115868 MsG0580026244.01.T01:CDS
TGATCATCCGACAGTTTCCC+AGG 0.489154 5:-33116158 None:intergenic
AACTAGTTTCGAAGATTGAC+GGG 0.490566 5:+33115504 MsG0580026244.01.T01:CDS
AGAGAAATGACAAAGTTGAC+TGG 0.491533 5:+33116612 MsG0580026244.01.T01:CDS
CAAGATGGGCTGTCAAAGAC+CGG 0.493342 5:+33116196 MsG0580026244.01.T01:CDS
GAATAACTCCTATACTCTCA+TGG 0.500379 5:-33115788 None:intergenic
GTTTCGAAGATTGACGGGAA+GGG 0.504847 5:+33115509 MsG0580026244.01.T01:CDS
GAGGAAATGTTGGCCCTACC+AGG 0.508892 5:+33116252 MsG0580026244.01.T01:CDS
GCATTGCAGTTATACCTGGT+AGG 0.509794 5:-33116266 None:intergenic
ACTGCTTTCCATGAGAGTAT+AGG 0.509877 5:+33115780 MsG0580026244.01.T01:CDS
TTGTCATTTCTCTTGAGACT+TGG 0.511045 5:-33116602 None:intergenic
CATCCTTTAAAGAAGTTGAT+GGG 0.514420 5:+33115090 MsG0580026244.01.T01:CDS
TGATAATGATTTAGCACCAA+AGG 0.516884 5:+33115046 MsG0580026244.01.T01:CDS
AGAGCAAGTCCTTGTTGTGT+TGG 0.520077 5:-33115738 None:intergenic
GCGCTTTGCCGGCATATGAT+GGG 0.527480 5:+33115345 MsG0580026244.01.T01:CDS
GATATCGGTGGAGGAGCTGT+TGG 0.528041 5:+33115686 MsG0580026244.01.T01:CDS
CTTGCAACAATTACTTCATG+TGG 0.531075 5:-33115137 None:intergenic
ATGGGGAGAAGCAAAGATAT+CGG 0.538077 5:+33115671 MsG0580026244.01.T01:CDS
GAGGAGCTGTTGGATTGGAG+AGG 0.550186 5:+33115696 MsG0580026244.01.T01:CDS
CCAGATTCTGGTGGACAAGA+AGG 0.553420 5:+33115164 MsG0580026244.01.T01:CDS
CTTCGCGTCACGATCGCCAA+TGG 0.561844 5:+33116697 MsG0580026244.01.T01:CDS
AAACTAGTTTCGAAGATTGA+CGG 0.563664 5:+33115503 MsG0580026244.01.T01:CDS
CCAAAACTAAAGCTTGGTGA+TGG 0.566399 5:+33116651 MsG0580026244.01.T01:CDS
CATTGCAGTTATACCTGGTA+GGG 0.568372 5:-33116265 None:intergenic
GAACGAAAAGCCATCATCGA+AGG 0.569372 5:+33116221 MsG0580026244.01.T01:CDS
TGGCCATCTAGAAGTTCCAT+TGG 0.569890 5:-33116713 None:intergenic
TCTCTAGCAACATCCTTAGA+GGG 0.573670 5:-33115278 None:intergenic
GAGAAATGACAAAGTTGACT+GGG 0.575964 5:+33116613 MsG0580026244.01.T01:CDS
ATTTGCTAGTTGGAATTGGG+AGG 0.582837 5:-33115427 None:intergenic
GCAACATCCTTAGAGGGATG+AGG 0.583299 5:-33115272 None:intergenic
AATCATTATCATAAATAAGA+CGG 0.583342 5:-33115034 None:intergenic
TGTGAAGGACAGAAGTTCCT+GGG 0.585013 5:+33116141 MsG0580026244.01.T01:intron
AGTTTCGAAGATTGACGGGA+AGG 0.591637 5:+33115508 MsG0580026244.01.T01:CDS
AAACTAAAGCTTGGTGATGG+AGG 0.594557 5:+33116654 MsG0580026244.01.T01:CDS
AGAAGCAAAGATATCGGTGG+AGG 0.598026 5:+33115677 MsG0580026244.01.T01:CDS
GGTGTCATGAGAGGAAATGT+TGG 0.600741 5:+33116242 MsG0580026244.01.T01:CDS
CATTGGCGATCGTGACGCGA+AGG 0.601741 5:-33116696 None:intergenic
TTCTTAGAGACCTTTCTCAA+AGG 0.606629 5:+33115835 MsG0580026244.01.T01:CDS
CACATGAAGTAATTGTTGCA+AGG 0.609332 5:+33115138 MsG0580026244.01.T01:CDS
GAAACTGTCGGATGATCAGA+CGG 0.623032 5:+33116163 MsG0580026244.01.T01:CDS
ATCATCGAAGGTGTCATGAG+AGG 0.629665 5:+33116233 MsG0580026244.01.T01:CDS
AAGATGGGCTGTCAAAGACC+GGG 0.633687 5:+33116197 MsG0580026244.01.T01:CDS
AGAAGTTCCTGGGAAACTGT+CGG 0.634410 5:+33116151 MsG0580026244.01.T01:CDS
ACAAGGACTTGCTCTCAATG+TGG 0.644006 5:+33115746 MsG0580026244.01.T01:CDS
AGTCTTAGACCAACACAACA+AGG 0.644925 5:+33115729 MsG0580026244.01.T01:CDS
GGAAGAAGCTTATTTCAACA+AGG 0.658895 5:+33115111 MsG0580026244.01.T01:CDS
CTTTGCCGGCATATGATGGG+AGG 0.659001 5:+33115348 MsG0580026244.01.T01:CDS
GGGAGAAGCAAAGATATCGG+TGG 0.666198 5:+33115674 MsG0580026244.01.T01:CDS
GCAAGGAGACCAGATTCTGG+TGG 0.670025 5:+33115155 MsG0580026244.01.T01:CDS
AAAGGTCTCTAAGAAACTCG+AGG 0.671596 5:-33115827 None:intergenic
TTGGCAAGGAGAGAGATCAC+AGG 0.686931 5:-33115188 None:intergenic
CATTCTATTCGAGTTCAATG+GGG 0.697995 5:+33115654 MsG0580026244.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATTATCATAAATAAGA+CGG - Chr5:33115037-33115056 None:intergenic 15.0%
!!! TTATCATTTTGGAATTCAAT+TGG - Chr5:33115389-33115408 None:intergenic 20.0%
! AATCAAACTTCACCAAAAAA+TGG - Chr5:33115214-33115233 None:intergenic 25.0%
! ATAACTATGACATTCAATTC+AGG + Chr5:33116052-33116071 MsG0580026244.01.T01:intron 25.0%
! TAGAGAAATCAAGCATAAAT+TGG + Chr5:33115295-33115314 MsG0580026244.01.T01:CDS 25.0%
!! GTGAACAACATAAACTTTTT+CGG + Chr5:33115471-33115490 MsG0580026244.01.T01:CDS 25.0%
!! TAACAATGCTGAGATTTTAT+CGG + Chr5:33115322-33115341 MsG0580026244.01.T01:CDS 25.0%
AAACTTCACCAAAAAATGGT+TGG - Chr5:33115210-33115229 None:intergenic 30.0%
ACTAAATACTTGAGCAAAGA+AGG + Chr5:33115536-33115555 MsG0580026244.01.T01:CDS 30.0%
CCACGTAATCACTTAATAAA+CGG - Chr5:33116448-33116467 None:intergenic 30.0%
TCACTTAATAAACGGTTTCA+CGG - Chr5:33116440-33116459 None:intergenic 30.0%
TTATTAAGTGATTACGTGGA+CGG + Chr5:33116449-33116468 MsG0580026244.01.T01:intron 30.0%
! AAAAGTAATCCTTTTGACAG+TGG + Chr5:33116555-33116574 MsG0580026244.01.T01:intron 30.0%
! AAACTAGTTTCGAAGATTGA+CGG + Chr5:33115503-33115522 MsG0580026244.01.T01:CDS 30.0%
! AACAATGCTGAGATTTTATC+GGG + Chr5:33115323-33115342 MsG0580026244.01.T01:CDS 30.0%
! AGAACTCAAGCTTATCATTT+TGG - Chr5:33115400-33115419 None:intergenic 30.0%
! CATCCTTTAAAGAAGTTGAT+GGG + Chr5:33115090-33115109 MsG0580026244.01.T01:CDS 30.0%
! TCATTCTATTCGAGTTCAAT+GGG + Chr5:33115653-33115672 MsG0580026244.01.T01:CDS 30.0%
! TGATAATGATTTAGCACCAA+AGG + Chr5:33115046-33115065 MsG0580026244.01.T01:CDS 30.0%
AAAGCATGTAGATAATCCTG+CGG - Chr5:33115581-33115600 None:intergenic 35.0%
ACAGGTATACATTTCTCAGT+CGG - Chr5:33115626-33115645 None:intergenic 35.0%
ACGGCAAAAATACTCAAGAT+GGG + Chr5:33116182-33116201 MsG0580026244.01.T01:CDS 35.0%
ACTGAGAAATGTATACCTGT+TGG + Chr5:33115626-33115645 MsG0580026244.01.T01:intron 35.0%
AGAAATGTATACCTGTTGGA+AGG + Chr5:33115630-33115649 MsG0580026244.01.T01:intron 35.0%
AGAGAAATGACAAAGTTGAC+TGG + Chr5:33116612-33116631 MsG0580026244.01.T01:CDS 35.0%
AGATTCTTTCCATCTCTATC+AGG - Chr5:33116003-33116022 None:intergenic 35.0%
AGCTAACTGTGAAGAAAGAA+AGG + Chr5:33115868-33115887 MsG0580026244.01.T01:CDS 35.0%
ATAGAGATGGAAAGAATCTG+AGG + Chr5:33116004-33116023 MsG0580026244.01.T01:intron 35.0%
ATGACATTCAATTCAGGAAG+TGG + Chr5:33116058-33116077 MsG0580026244.01.T01:intron 35.0%
CACATGAAGTAATTGTTGCA+AGG + Chr5:33115138-33115157 MsG0580026244.01.T01:CDS 35.0%
CCGTTTATTAAGTGATTACG+TGG + Chr5:33116445-33116464 MsG0580026244.01.T01:intron 35.0%
CTTCCCATCAACTTCTTTAA+AGG - Chr5:33115096-33115115 None:intergenic 35.0%
CTTGCAACAATTACTTCATG+TGG - Chr5:33115140-33115159 None:intergenic 35.0%
GAATAACTCCTATACTCTCA+TGG - Chr5:33115791-33115810 None:intergenic 35.0%
GAGAAATGACAAAGTTGACT+GGG + Chr5:33116613-33116632 MsG0580026244.01.T01:CDS 35.0%
GGAAGAAGCTTATTTCAACA+AGG + Chr5:33115111-33115130 MsG0580026244.01.T01:CDS 35.0%
GTTTGCTTCTGAATTTCCTT+TGG - Chr5:33115065-33115084 None:intergenic 35.0%
TGTTGTTCACGTTTCTCATA+AGG - Chr5:33115461-33115480 None:intergenic 35.0%
TTCTTAGAGACCTTTCTCAA+AGG + Chr5:33115835-33115854 MsG0580026244.01.T01:CDS 35.0%
! AACTAGTTTCGAAGATTGAC+GGG + Chr5:33115504-33115523 MsG0580026244.01.T01:CDS 35.0%
! CATTCTATTCGAGTTCAATG+GGG + Chr5:33115654-33115673 MsG0580026244.01.T01:CDS 35.0%
! CCATCAACTTCTTTAAAGGA+TGG - Chr5:33115092-33115111 None:intergenic 35.0%
! CCATCCTTTAAAGAAGTTGA+TGG + Chr5:33115089-33115108 MsG0580026244.01.T01:CDS 35.0%
! GGAGTTTGAATTCATTTTCC+TGG - Chr5:33116584-33116603 None:intergenic 35.0%
! GTCATTCTATTCGAGTTCAA+TGG + Chr5:33115652-33115671 MsG0580026244.01.T01:CDS 35.0%
! TAAACCTTGTTATCTCCCTA+TGG + Chr5:33116104-33116123 MsG0580026244.01.T01:intron 35.0%
! TTGTCATTTCTCTTGAGACT+TGG - Chr5:33116605-33116624 None:intergenic 35.0%
!! TCGATTTGCTAGTTGGAATT+GGG - Chr5:33115433-33115452 None:intergenic 35.0%
AATTACTCCTCATCCCTCTA+AGG + Chr5:33115265-33115284 MsG0580026244.01.T01:CDS 40.0%
AGATCACACAAAGCTCCATA+GGG - Chr5:33116122-33116141 None:intergenic 40.0%
AGGAGGCAACTGAAAATCTT+TGG + Chr5:33115974-33115993 MsG0580026244.01.T01:intron 40.0%
AGTCTTAGACCAACACAACA+AGG + Chr5:33115729-33115748 MsG0580026244.01.T01:CDS 40.0%
ATCTCTGAACGGTTTCTCTA+TGG - Chr5:33115934-33115953 None:intergenic 40.0%
ATGGGGAGAAGCAAAGATAT+CGG + Chr5:33115671-33115690 MsG0580026244.01.T01:CDS 40.0%
CATTGCAGTTATACCTGGTA+GGG - Chr5:33116268-33116287 None:intergenic 40.0%
CCTCATAAAAACCAGAACAC+TGG + Chr5:33116386-33116405 MsG0580026244.01.T01:intron 40.0%
GAATAGAATGACCTTCCAAC+AGG - Chr5:33115644-33115663 None:intergenic 40.0%
GACGGCAAAAATACTCAAGA+TGG + Chr5:33116181-33116200 MsG0580026244.01.T01:CDS 40.0%
GCAGGATTATCTACATGCTT+TGG + Chr5:33115580-33115599 MsG0580026244.01.T01:intron 40.0%
GTTAAGATACTCCAGTGTTC+TGG - Chr5:33116400-33116419 None:intergenic 40.0%
TACCCTCCAAAACTAAAGCT+TGG + Chr5:33116645-33116664 MsG0580026244.01.T01:CDS 40.0%
TCACCAAAAAATGGTTGGCA+AGG - Chr5:33115205-33115224 None:intergenic 40.0%
TCTCTAGCAACATCCTTAGA+GGG - Chr5:33115281-33115300 None:intergenic 40.0%
TGATTACGTGGACGGTTAAA+TGG + Chr5:33116457-33116476 MsG0580026244.01.T01:intron 40.0%
TGCTCTCAATGTGGATTTCT+CGG + Chr5:33115755-33115774 MsG0580026244.01.T01:CDS 40.0%
TTCTCTAGCAACATCCTTAG+AGG - Chr5:33115282-33115301 None:intergenic 40.0%
! AAAGGTCTCTAAGAAACTCG+AGG - Chr5:33115830-33115849 None:intergenic 40.0%
! ACTGCTTTCCATGAGAGTAT+AGG + Chr5:33115780-33115799 MsG0580026244.01.T01:CDS 40.0%
! ATGCTTTGGATGTAGTCTTG+AGG + Chr5:33115594-33115613 MsG0580026244.01.T01:intron 40.0%
! ATTTGCTAGTTGGAATTGGG+AGG - Chr5:33115430-33115449 None:intergenic 40.0%
! CCAAAACTAAAGCTTGGTGA+TGG + Chr5:33116651-33116670 MsG0580026244.01.T01:CDS 40.0%
! CTCCTACTAATTTGCAAGCA+TGG - Chr5:33116084-33116103 None:intergenic 40.0%
! CTTTGGTTTCCTGATAGAGA+TGG + Chr5:33115991-33116010 MsG0580026244.01.T01:intron 40.0%
! TGCTTTGGATGTAGTCTTGA+GGG + Chr5:33115595-33115614 MsG0580026244.01.T01:intron 40.0%
! TTTTTCCTCCCATCATATGC+CGG - Chr5:33115356-33115375 None:intergenic 40.0%
!! AAACTAAAGCTTGGTGATGG+AGG + Chr5:33116654-33116673 MsG0580026244.01.T01:CDS 40.0%
!! CCATCACCAAGCTTTAGTTT+TGG - Chr5:33116654-33116673 None:intergenic 40.0%
!! GTCGATTTGCTAGTTGGAAT+TGG - Chr5:33115434-33115453 None:intergenic 40.0%
!!! CACCAAGCTTTAGTTTTGGA+GGG - Chr5:33116650-33116669 None:intergenic 40.0%
!!! CCAGTGTTCTGGTTTTTATG+AGG - Chr5:33116389-33116408 None:intergenic 40.0%
!!! TCACCAAGCTTTAGTTTTGG+AGG - Chr5:33116651-33116670 None:intergenic 40.0%
!!! TCTCCTTGCCAACCATTTTT+TGG + Chr5:33115199-33115218 MsG0580026244.01.T01:CDS 40.0%
ACGGTTTCACGGTTTGTGAA+TGG - Chr5:33116429-33116448 None:intergenic 45.0%
AGAAGCAAAGATATCGGTGG+AGG + Chr5:33115677-33115696 MsG0580026244.01.T01:CDS 45.0%
AGAAGTTCCTGGGAAACTGT+CGG + Chr5:33116151-33116170 MsG0580026244.01.T01:CDS 45.0%
AGCTCCATAGGGAGATAACA+AGG - Chr5:33116111-33116130 None:intergenic 45.0%
AGTTTCGAAGATTGACGGGA+AGG + Chr5:33115508-33115527 MsG0580026244.01.T01:CDS 45.0%
CACGGTTTGTGAATGGATGT+TGG - Chr5:33116422-33116441 None:intergenic 45.0%
CAGATCACACAAAGCTCCAT+AGG - Chr5:33116123-33116142 None:intergenic 45.0%
CTAGACACGGTATCTCTGAA+CGG - Chr5:33115945-33115964 None:intergenic 45.0%
GAAACTGTCGGATGATCAGA+CGG + Chr5:33116163-33116182 MsG0580026244.01.T01:CDS 45.0%
GAACGAAAAGCCATCATCGA+AGG + Chr5:33116221-33116240 MsG0580026244.01.T01:CDS 45.0%
GCATTGCAGTTATACCTGGT+AGG - Chr5:33116269-33116288 None:intergenic 45.0%
GGCCATGCTTGCAAATTAGT+AGG + Chr5:33116079-33116098 MsG0580026244.01.T01:intron 45.0%
GTTTCGAAGATTGACGGGAA+GGG + Chr5:33115509-33115528 MsG0580026244.01.T01:CDS 45.0%
TCCTCAGTTAAGCCTAGACA+CGG - Chr5:33115958-33115977 None:intergenic 45.0%
TCCTGGTTACCACTGTCAAA+AGG - Chr5:33116567-33116586 None:intergenic 45.0%
TCTCATGACACCTTCGATGA+TGG - Chr5:33116234-33116253 None:intergenic 45.0%
TGAGCAAAGAAGGTGACGAA+GGG + Chr5:33115546-33115565 MsG0580026244.01.T01:intron 45.0%
TGCTGCATTGCAGTTATACC+TGG - Chr5:33116273-33116292 None:intergenic 45.0%
TGTAGATAATCCTGCGGAAG+TGG - Chr5:33115575-33115594 None:intergenic 45.0%
TGTGAAGGACAGAAGTTCCT+GGG + Chr5:33116141-33116160 MsG0580026244.01.T01:intron 45.0%
TTGAGCAAAGAAGGTGACGA+AGG + Chr5:33115545-33115564 MsG0580026244.01.T01:intron 45.0%
TTTCGAAGATTGACGGGAAG+GGG + Chr5:33115510-33115529 MsG0580026244.01.T01:CDS 45.0%
! ACAAGGACTTGCTCTCAATG+TGG + Chr5:33115746-33115765 MsG0580026244.01.T01:CDS 45.0%
! AGAGCAAGTCCTTGTTGTGT+TGG - Chr5:33115741-33115760 None:intergenic 45.0%
! ATGATGGCTTTTCGTTCTCC+CGG - Chr5:33116218-33116237 None:intergenic 45.0%
! GAGCTTTGTGTGATCTGTGA+AGG + Chr5:33116126-33116145 MsG0580026244.01.T01:intron 45.0%
! GAGTTGTCTGCCTTTGAGAA+AGG - Chr5:33115848-33115867 None:intergenic 45.0%
! GGTGTCATGAGAGGAAATGT+TGG + Chr5:33116242-33116261 MsG0580026244.01.T01:CDS 45.0%
! TCCTTTTGACAGTGGTAACC+AGG + Chr5:33116563-33116582 MsG0580026244.01.T01:intron 45.0%
! TCGAGGCGTTTCTGCAAATA+CGG - Chr5:33115813-33115832 None:intergenic 45.0%
!! ATCATCGAAGGTGTCATGAG+AGG + Chr5:33116233-33116252 MsG0580026244.01.T01:CDS 45.0%
!! GGTGATGTCGATTTGCTAGT+TGG - Chr5:33115440-33115459 None:intergenic 45.0%
ACCGTGTCTAGGCTTAACTG+AGG + Chr5:33115954-33115973 MsG0580026244.01.T01:intron 50.0%
CCTTCTTGTCCACCAGAATC+TGG - Chr5:33115167-33115186 None:intergenic 50.0%
CGTTCAGAGATACCGTGTCT+AGG + Chr5:33115943-33115962 MsG0580026244.01.T01:intron 50.0%
CTGTGAAGGACAGAAGTTCC+TGG + Chr5:33116140-33116159 MsG0580026244.01.T01:intron 50.0%
GCAACATCCTTAGAGGGATG+AGG - Chr5:33115275-33115294 None:intergenic 50.0%
GGGAGAAGCAAAGATATCGG+TGG + Chr5:33115674-33115693 MsG0580026244.01.T01:CDS 50.0%
GTGTCTAGGCTTAACTGAGG+AGG + Chr5:33115957-33115976 MsG0580026244.01.T01:intron 50.0%
GTTGCAAGGAGACCAGATTC+TGG + Chr5:33115152-33115171 MsG0580026244.01.T01:CDS 50.0%
TGATCATCCGACAGTTTCCC+AGG - Chr5:33116161-33116180 None:intergenic 50.0%
TTGGCAAGGAGAGAGATCAC+AGG - Chr5:33115191-33115210 None:intergenic 50.0%
! CCAGATTCTGGTGGACAAGA+AGG + Chr5:33115164-33115183 MsG0580026244.01.T01:CDS 50.0%
! GATTTTATCGGGCGCTTTGC+CGG + Chr5:33115334-33115353 MsG0580026244.01.T01:CDS 50.0%
!! AAGATGGGCTGTCAAAGACC+GGG + Chr5:33116197-33116216 MsG0580026244.01.T01:CDS 50.0%
!! CAAGATGGGCTGTCAAAGAC+CGG + Chr5:33116196-33116215 MsG0580026244.01.T01:CDS 50.0%
!! GGACGGTTAAATGGACTACG+TGG + Chr5:33116466-33116485 MsG0580026244.01.T01:intron 50.0%
GAGGAAATGTTGGCCCTACC+AGG + Chr5:33116252-33116271 MsG0580026244.01.T01:CDS 55.0%
GATATCGGTGGAGGAGCTGT+TGG + Chr5:33115686-33115705 MsG0580026244.01.T01:CDS 55.0%
GCAAGGAGACCAGATTCTGG+TGG + Chr5:33115155-33115174 MsG0580026244.01.T01:CDS 55.0%
GCGCTTTGCCGGCATATGAT+GGG + Chr5:33115345-33115364 MsG0580026244.01.T01:CDS 55.0%
! CTTTGCCGGCATATGATGGG+AGG + Chr5:33115348-33115367 MsG0580026244.01.T01:CDS 55.0%
! GAGGAGCTGTTGGATTGGAG+AGG + Chr5:33115696-33115715 MsG0580026244.01.T01:CDS 55.0%
CATTGGCGATCGTGACGCGA+AGG - Chr5:33116699-33116718 None:intergenic 60.0%
CGAAGGGATTCCACTTCCGC+AGG + Chr5:33115562-33115581 MsG0580026244.01.T01:intron 60.0%
CGGTGGAGGAGCTGTTGGAT+TGG + Chr5:33115691-33115710 MsG0580026244.01.T01:CDS 60.0%
CTTCGCGTCACGATCGCCAA+TGG + Chr5:33116697-33116716 MsG0580026244.01.T01:CDS 60.0%
GGCGCTTTGCCGGCATATGA+TGG + Chr5:33115344-33115363 MsG0580026244.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 33115026 33116728 33115026 ID=MsG0580026244.01;Name=MsG0580026244.01
Chr5 mRNA 33115026 33116728 33115026 ID=MsG0580026244.01.T01;Parent=MsG0580026244.01;Name=MsG0580026244.01.T01;_AED=0.18;_eAED=0.18;_QI=0|0.33|0.25|1|1|1|4|0|350
Chr5 exon 33115026 33115557 33115026 ID=MsG0580026244.01.T01:exon:25194;Parent=MsG0580026244.01.T01
Chr5 exon 33115652 33115892 33115652 ID=MsG0580026244.01.T01:exon:25195;Parent=MsG0580026244.01.T01
Chr5 exon 33116148 33116273 33116148 ID=MsG0580026244.01.T01:exon:25196;Parent=MsG0580026244.01.T01
Chr5 exon 33116575 33116728 33116575 ID=MsG0580026244.01.T01:exon:25197;Parent=MsG0580026244.01.T01
Chr5 CDS 33115026 33115557 33115026 ID=MsG0580026244.01.T01:cds;Parent=MsG0580026244.01.T01
Chr5 CDS 33115652 33115892 33115652 ID=MsG0580026244.01.T01:cds;Parent=MsG0580026244.01.T01
Chr5 CDS 33116148 33116273 33116148 ID=MsG0580026244.01.T01:cds;Parent=MsG0580026244.01.T01
Chr5 CDS 33116575 33116728 33116575 ID=MsG0580026244.01.T01:cds;Parent=MsG0580026244.01.T01
Gene Sequence

>MsG0580026244.01.T01

ATGATGAACCGTCTTATTTATGATAATGATTTAGCACCAAAGGAAATTCAGAAGCAAACTAGACCATCCTTTAAAGAAGTTGATGGGAAGAAGCTTATTTCAACAAGGAAGCCACATGAAGTAATTGTTGCAAGGAGACCAGATTCTGGTGGACAAGAAGGTCCTGTGATCTCTCTCCTTGCCAACCATTTTTTGGTGAAGTTTGATTCATCACAAAAAATATATCATTACAATGTTGAAATTACTCCTCATCCCTCTAAGGATGTTGCTAGAGAAATCAAGCATAAATTGGTAAATAACAATGCTGAGATTTTATCGGGCGCTTTGCCGGCATATGATGGGAGGAAAAATCTTTATAGTCCAATTGAATTCCAAAATGATAAGCTTGAGTTCTACATAAGCCTCCCAATTCCAACTAGCAAATCGACATCACCTTATGAGAAACGTGAACAACATAAACTTTTTCGGATAAATATCAAACTAGTTTCGAAGATTGACGGGAAGGGGTTGACTAAATACTTGAGCAAAGAAGGTCATTCTATTCGAGTTCAATGGGGAGAAGCAAAGATATCGGTGGAGGAGCTGTTGGATTGGAGAGGCTTCTTTCAGAGTCTTAGACCAACACAACAAGGACTTGCTCTCAATGTGGATTTCTCGGTAACTGCTTTCCATGAGAGTATAGGAGTTATTCCGTATTTGCAGAAACGCCTCGAGTTTCTTAGAGACCTTTCTCAAAGGCAGACAACTCAGCTAACTGTGAAGAAAGAAAGGAAGACAGAAGTTCCTGGGAAACTGTCGGATGATCAGACGGCAAAAATACTCAAGATGGGCTGTCAAAGACCGGGAGAACGAAAAGCCATCATCGAAGGTGTCATGAGAGGAAATGTTGGCCCTACCAGTGGTAACCAGGAAAATGAATTCAAACTCCAAGTCTCAAGAGAAATGACAAAGTTGACTGGGAGAATTCTTTACCCTCCAAAACTAAAGCTTGGTGATGGAGGTCATGTGAGAAATCTGACTCCTTCGCGTCACGATCGCCAATGGAACTTCTAG
Protein sequence

>MsG0580026244.01.T01

MMNRLIYDNDLAPKEIQKQTRPSFKEVDGKKLISTRKPHEVIVARRPDSGGQEGPVISLLANHFLVKFDSSQKIYHYNVEITPHPSKDVAREIKHKLVNNNAEILSGALPAYDGRKNLYSPIEFQNDKLEFYISLPIPTSKSTSPYEKREQHKLFRINIKLVSKIDGKGLTKYLSKEGHSIRVQWGEAKISVEELLDWRGFFQSLRPTQQGLALNVDFSVTAFHESIGVIPYLQKRLEFLRDLSQRQTTQLTVKKERKTEVPGKLSDDQTAKILKMGCQRPGERKAIIEGVMRGNVGPTSGNQENEFKLQVSREMTKLTGRILYPPKLKLGDGGHVRNLTPSRHDRQWNF*