Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027997.01.T01 | XP_003614528.1 | 87.764 | 237 | 28 | 1 | 1 | 236 | 1 | 237 | 3.17E-152 | 432 |
| MsG0580027997.01.T02 | XP_003614528.1 | 87.444 | 223 | 27 | 1 | 1 | 222 | 15 | 237 | 4.60E-142 | 406 |
Co-expression Network
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380013711.01 | MsG0580027997.01 | PPI |
| MsG0380013711.01 | MsG0580027997.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027997.01.T01 | MTR_5g055100 | 87.764 | 237 | 28 | 1 | 1 | 236 | 1 | 237 | 3.84e-156 | 432 |
| MsG0580027997.01.T01 | MTR_2g085280 | 81.356 | 236 | 43 | 1 | 1 | 235 | 1 | 236 | 6.45e-142 | 397 |
| MsG0580027997.01.T01 | MTR_2g085250 | 81.356 | 236 | 43 | 1 | 1 | 235 | 1 | 236 | 2.35e-141 | 395 |
| MsG0580027997.01.T01 | MTR_5g045560 | 76.889 | 225 | 52 | 0 | 1 | 225 | 1 | 225 | 1.98e-126 | 357 |
| MsG0580027997.01.T01 | MTR_5g053390 | 76.000 | 225 | 54 | 0 | 1 | 225 | 1 | 225 | 1.49e-124 | 353 |
| MsG0580027997.01.T01 | MTR_1g077360 | 70.222 | 225 | 67 | 0 | 1 | 225 | 1 | 225 | 1.85e-113 | 325 |
| MsG0580027997.01.T01 | MTR_1g075600 | 70.222 | 225 | 67 | 0 | 1 | 225 | 1 | 225 | 2.55e-113 | 324 |
| MsG0580027997.01.T01 | MTR_8g043650 | 64.557 | 237 | 83 | 1 | 1 | 236 | 1 | 237 | 1.63e-111 | 320 |
| MsG0580027997.01.T01 | MTR_1g105920 | 67.421 | 221 | 72 | 0 | 1 | 221 | 1 | 221 | 1.58e-108 | 312 |
| MsG0580027997.01.T01 | MTR_1g105910 | 63.556 | 225 | 82 | 0 | 1 | 225 | 1 | 225 | 9.05e-107 | 308 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027997.01.T01 | AT5G60440 | 48.677 | 189 | 90 | 3 | 5 | 190 | 3 | 187 | 4.38e-52 | 171 |
| MsG0580027997.01.T01 | AT1G65360 | 41.410 | 227 | 118 | 6 | 5 | 225 | 3 | 220 | 3.76e-42 | 143 |
| MsG0580027997.01.T01 | AT2G24840 | 48.734 | 158 | 76 | 2 | 9 | 163 | 63 | 218 | 3.00e-39 | 137 |
| MsG0580027997.01.T01 | AT1G01530 | 40.526 | 190 | 105 | 3 | 4 | 192 | 2 | 184 | 6.67e-38 | 132 |
| MsG0580027997.01.T01 | AT4G36590 | 36.820 | 239 | 122 | 8 | 5 | 226 | 3 | 229 | 1.74e-34 | 124 |
| MsG0580027997.01.T01 | AT3G04100 | 31.056 | 161 | 107 | 2 | 2 | 158 | 7 | 167 | 6.37e-26 | 100 |
| MsG0580027997.01.T01 | AT1G72350 | 35.542 | 166 | 69 | 5 | 9 | 169 | 43 | 175 | 3.20e-22 | 91.3 |
| MsG0580027997.01.T01 | AT2G34440 | 38.739 | 111 | 67 | 1 | 9 | 119 | 2 | 111 | 4.18e-21 | 87.0 |
| MsG0580027997.01.T01 | AT3G66656 | 55.882 | 68 | 30 | 0 | 9 | 76 | 2 | 69 | 1.23e-20 | 85.9 |
| MsG0580027997.01.T01 | AT4G37940 | 32.948 | 173 | 85 | 5 | 9 | 154 | 2 | 170 | 3.29e-19 | 83.2 |
Find 56 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAATGTGGTTGCAACAT+AGG | 0.466580 | 5:+72718216 | None:intergenic |
| AAGGTGGACATGGTCCCCAT+GGG | 0.619822 | 5:-72718173 | MsG0580027997.01.T01:CDS |
| AATATGGGAGAAGGTGGACA+TGG | 0.617316 | 5:-72718183 | MsG0580027997.01.T01:CDS |
| AATGAAAATACTTTCTCAGC+AGG | 0.517037 | 5:+72718684 | None:intergenic |
| ACAAATGTGGTTGCAACATA+GGG | 0.521813 | 5:+72718217 | None:intergenic |
| ACCATAAAATGGAAGGGTTT+GGG | 0.373429 | 5:+72718326 | None:intergenic |
| ACCATTGAGCTCGCGCACCT+TGG | 0.535093 | 5:+72718563 | None:intergenic |
| ACCTTATTCATCTCATAAAT+GGG | 0.419947 | 5:+72718438 | None:intergenic |
| ACGCAATATATGCACCACAA+AGG | 0.628806 | 5:+72718721 | None:intergenic |
| ACTGGTATGAAAGATGCAAT+CGG | 0.573096 | 5:-72718534 | MsG0580027997.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGAAAATACTTTCTCAGC+AGG | + | Chr5:72718335-72718354 | None:intergenic | 30.0% | |
| ATTTCCACCATAAAATGGAA+GGG | + | Chr5:72718699-72718718 | None:intergenic | 30.0% | |
| CCAACTTGAATTATACAAGA+AGG | - | Chr5:72718601-72718620 | MsG0580027997.01.T01:CDS | 30.0% | |
| CCTTCTTGTATAATTCAAGT+TGG | + | Chr5:72718604-72718623 | None:intergenic | 30.0% | |
| GACCTTATTCATCTCATAAA+TGG | + | Chr5:72718582-72718601 | None:intergenic | 30.0% | |
| TCCCATTTATGAGATGAATA+AGG | - | Chr5:72718577-72718596 | MsG0580027997.01.T01:CDS | 30.0% | |
| TCTATGATCGTTTCAACATT+TGG | + | Chr5:72718365-72718384 | None:intergenic | 30.0% | |
| TGACATCATGCAATTAATTG+AGG | - | Chr5:72718418-72718437 | MsG0580027997.01.T01:CDS | 30.0% | |
| TTGTTGAAACCAAACAAATG+TGG | + | Chr5:72718815-72718834 | None:intergenic | 30.0% | |
| AACAAATGTGGTTGCAACAT+AGG | + | Chr5:72718803-72718822 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 72718164 | 72718874 | 72718164 | ID=MsG0580027997.01;Name=MsG0580027997.01 |
| Chr5 | mRNA | 72718164 | 72718874 | 72718164 | ID=MsG0580027997.01.T01;Parent=MsG0580027997.01;Name=MsG0580027997.01.T01;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|236 |
| Chr5 | exon | 72718164 | 72718874 | 72718164 | ID=MsG0580027997.01.T01:exon:4631;Parent=MsG0580027997.01.T01 |
| Chr5 | CDS | 72718164 | 72718874 | 72718164 | ID=MsG0580027997.01.T01:cds;Parent=MsG0580027997.01.T01 |
| Chr5 | mRNA | 72718164 | 72718832 | 72718164 | ID=MsG0580027997.01.T02;Parent=MsG0580027997.01;Name=MsG0580027997.01.T02;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|0|1|0|222 |
| Chr5 | exon | 72718164 | 72718832 | 72718164 | ID=MsG0580027997.01.T02:exon:4632;Parent=MsG0580027997.01.T02 |
| Chr5 | CDS | 72718164 | 72718832 | 72718164 | ID=MsG0580027997.01.T02:cds;Parent=MsG0580027997.01.T02 |
| Gene Sequence |
>MsG0580027997.01.T02 ATGAAAAAGATGAGCAATGAAAGTAACTTGCAAGTGACTTTCTCGAAGCGCCGTAGTGGGCTCTTCAAGAAAGCTAGCGAGCTTTGCACCCTTTGTGGTGCATATATTGCGTTAATCATATTCTCGCCTGCTGAGAAAGTATTTTCATTTGGTCATCCAAATGTTGAAACGATCATAGATCGTTATCTCTCTCAAGTTCCAGCCCAAAATGATGACATCATGCAATTAATTGAGGATTACCGTAGAGCCAAGGTGCGCGAGCTCAATGGTATATTGACTGGTATGAAAGATGCAATCGGCATCGATAAGAACCGTGAGAATGAGCTAATCCAGGTGCGAAAGACAAATCAGGAACATTTTTGGTGGACTCGTCCCATTTATGAGATGAATAAGGTCCAACTTGAATTATACAAGAAGGCTTTAGAGGATCTTCTTAAACTTGTTGCTCAACATGCTGATAGGGTTGAAATGCAGGGTACTTCTACCCAAACCCTTCCATTTTATGGTGGAAATGGTTCATCCTCTGGCATGCCTCTTGAGCATCAGCCAAATCCTCAACAAGATTCAACCTTTCCAGCAGAGTTTTTTCATAACCCTATGTTGCAACCACATTTGTTTGGTTTCAACAATATGGGAGAAGGTGGACATGGTCCCCATGGGTTCTTTTGA >MsG0580027997.01.T01 ATGTCAAGCGGAAAGAAAACTCAAGGTCGTAAGAAGATTGAGATGAAAAAGATGAGCAATGAAAGTAACTTGCAAGTGACTTTCTCGAAGCGCCGTAGTGGGCTCTTCAAGAAAGCTAGCGAGCTTTGCACCCTTTGTGGTGCATATATTGCGTTAATCATATTCTCGCCTGCTGAGAAAGTATTTTCATTTGGTCATCCAAATGTTGAAACGATCATAGATCGTTATCTCTCTCAAGTTCCAGCCCAAAATGATGACATCATGCAATTAATTGAGGATTACCGTAGAGCCAAGGTGCGCGAGCTCAATGGTATATTGACTGGTATGAAAGATGCAATCGGCATCGATAAGAACCGTGAGAATGAGCTAATCCAGGTGCGAAAGACAAATCAGGAACATTTTTGGTGGACTCGTCCCATTTATGAGATGAATAAGGTCCAACTTGAATTATACAAGAAGGCTTTAGAGGATCTTCTTAAACTTGTTGCTCAACATGCTGATAGGGTTGAAATGCAGGGTACTTCTACCCAAACCCTTCCATTTTATGGTGGAAATGGTTCATCCTCTGGCATGCCTCTTGAGCATCAGCCAAATCCTCAACAAGATTCAACCTTTCCAGCAGAGTTTTTTCATAACCCTATGTTGCAACCACATTTGTTTGGTTTCAACAATATGGGAGAAGGTGGACATGGTCCCCATGGGTTCTTTTGA |
| Protein sequence |
>MsG0580027997.01.T02 MKKMSNESNLQVTFSKRRSGLFKKASELCTLCGAYIALIIFSPAEKVFSFGHPNVETIIDRYLSQVPAQNDDIMQLIEDYRRAKVRELNGILTGMKDAIGIDKNRENELIQVRKTNQEHFWWTRPIYEMNKVQLELYKKALEDLLKLVAQHADRVEMQGTSTQTLPFYGGNGSSSGMPLEHQPNPQQDSTFPAEFFHNPMLQPHLFGFNNMGEGGHGPHGFF* >MsG0580027997.01.T01 MSSGKKTQGRKKIEMKKMSNESNLQVTFSKRRSGLFKKASELCTLCGAYIALIIFSPAEKVFSFGHPNVETIIDRYLSQVPAQNDDIMQLIEDYRRAKVRELNGILTGMKDAIGIDKNRENELIQVRKTNQEHFWWTRPIYEMNKVQLELYKKALEDLLKLVAQHADRVEMQGTSTQTLPFYGGNGSSSGMPLEHQPNPQQDSTFPAEFFHNPMLQPHLFGFNNMGEGGHGPHGFF* |
