Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028129.01.T01 | XP_003615979.1 | 95.046 | 323 | 16 | 0 | 1 | 323 | 1 | 323 | 0 | 636 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580028129.01 | MsG0680034728.01 | 0.800815 | 1.207150e-48 | 5.674728e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028129.01.T01 | MTR_5g074740 | 95.046 | 323 | 16 | 0 | 1 | 323 | 1 | 323 | 0.0 | 636 |
| MsG0580028129.01.T01 | MTR_5g074760 | 93.789 | 322 | 20 | 0 | 1 | 322 | 1 | 322 | 0.0 | 628 |
| MsG0580028129.01.T01 | MTR_5g074710 | 86.068 | 323 | 45 | 0 | 1 | 323 | 1 | 323 | 0.0 | 584 |
| MsG0580028129.01.T01 | MTR_5g074860 | 71.293 | 317 | 91 | 0 | 4 | 320 | 8 | 324 | 3.62e-168 | 470 |
| MsG0580028129.01.T01 | MTR_5g074700 | 85.841 | 226 | 18 | 1 | 98 | 323 | 5 | 216 | 1.96e-140 | 396 |
| MsG0580028129.01.T01 | MTR_3g467600 | 62.813 | 320 | 117 | 2 | 4 | 321 | 5 | 324 | 4.06e-140 | 399 |
| MsG0580028129.01.T01 | MTR_5g074860 | 61.830 | 317 | 82 | 1 | 4 | 320 | 8 | 285 | 3.37e-136 | 388 |
| MsG0580028129.01.T01 | MTR_4g029170 | 59.385 | 325 | 124 | 5 | 1 | 321 | 1 | 321 | 5.49e-130 | 374 |
| MsG0580028129.01.T01 | MTR_4g029180 | 62.838 | 296 | 104 | 4 | 28 | 321 | 30 | 321 | 8.05e-125 | 360 |
| MsG0580028129.01.T01 | MTR_5g074970 | 54.348 | 322 | 137 | 7 | 5 | 321 | 10 | 326 | 2.67e-115 | 336 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028129.01.T01 | AT5G05340 | 55.960 | 302 | 125 | 5 | 23 | 321 | 28 | 324 | 1.13e-113 | 332 |
| MsG0580028129.01.T01 | AT1G14550 | 50.313 | 320 | 152 | 6 | 5 | 321 | 6 | 321 | 7.09e-102 | 302 |
| MsG0580028129.01.T01 | AT5G58390 | 51.572 | 318 | 145 | 6 | 8 | 321 | 4 | 316 | 6.32e-99 | 294 |
| MsG0580028129.01.T01 | AT2G18150 | 48.534 | 307 | 146 | 5 | 23 | 321 | 34 | 336 | 3.01e-98 | 293 |
| MsG0580028129.01.T01 | AT5G66390 | 50.654 | 306 | 139 | 5 | 25 | 322 | 33 | 334 | 1.41e-97 | 291 |
| MsG0580028129.01.T01 | AT5G58400 | 48.765 | 324 | 156 | 7 | 3 | 321 | 7 | 325 | 1.84e-97 | 291 |
| MsG0580028129.01.T01 | AT4G36430 | 48.860 | 307 | 145 | 5 | 23 | 321 | 28 | 330 | 3.29e-95 | 285 |
| MsG0580028129.01.T01 | AT2G18140 | 49.180 | 305 | 143 | 5 | 25 | 321 | 35 | 335 | 1.00e-94 | 285 |
| MsG0580028129.01.T01 | AT1G14540 | 49.169 | 301 | 147 | 5 | 23 | 321 | 19 | 315 | 2.90e-93 | 280 |
| MsG0580028129.01.T01 | AT5G06720 | 43.344 | 323 | 172 | 4 | 7 | 321 | 14 | 333 | 2.28e-89 | 271 |
Find 54 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACTTAGTTGTTCTATC+TGG | 0.291396 | 5:-75092759 | MsG0580028129.01.T01:CDS |
| AAAGCTCTTGATCAGAATGA+AGG | 0.528130 | 5:+75092535 | None:intergenic |
| AAGACATGCCCTAGAATCGG+CGG | 0.670665 | 5:-75092642 | MsG0580028129.01.T01:CDS |
| AAGGCTTTGTTGTATAAACA+AGG | 0.598286 | 5:-75092561 | MsG0580028129.01.T01:CDS |
| AATTACTCGTGAGCCACGCA+TGG | 0.562987 | 5:-75094209 | MsG0580028129.01.T01:CDS |
| AGAAAGACTTACTATGGCTA+CGG | 0.596519 | 5:+75093151 | None:intergenic |
| AGATGACACATCCTATTATA+AGG | 0.395008 | 5:-75092580 | MsG0580028129.01.T01:CDS |
| AGATTCAAGATTTGCAAGCA+GGG | 0.563486 | 5:-75092865 | MsG0580028129.01.T01:CDS |
| AGCAACGATGCTCCCATGCG+TGG | 0.625399 | 5:+75094196 | None:intergenic |
| AGTTGGGTGGTAAGCAATAT+TGG | 0.363597 | 5:-75092911 | MsG0580028129.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGACTTAGTTGTTCTATC+TGG | - | Chr5:75093958-75093977 | MsG0580028129.01.T01:intron | 30.0% | |
| AAGTTTGCAAGAAAAGTAGT+TGG | - | Chr5:75093139-75093158 | MsG0580028129.01.T01:intron | 30.0% | |
| AGATGACACATCCTATTATA+AGG | - | Chr5:75094137-75094156 | MsG0580028129.01.T01:intron | 30.0% | |
| AGTCGATGTATTCAAAATTG+CGG | + | Chr5:75093673-75093692 | None:intergenic | 30.0% | |
| AGTTGTGAGAAGTTGAAAAA+TGG | + | Chr5:75093904-75093923 | None:intergenic | 30.0% | |
| AGTTTGCAAGAAAAGTAGTT+GGG | - | Chr5:75093140-75093159 | MsG0580028129.01.T01:intron | 30.0% | |
| CAATTCAATTAGAGGATTTG+AGG | - | Chr5:75093453-75093472 | MsG0580028129.01.T01:intron | 30.0% | |
| GGAGTTTCTATGATTAAGAT+GGG | - | Chr5:75094282-75094301 | MsG0580028129.01.T01:CDS | 30.0% | |
| GTCAAACAATTAGGTATCAA+TGG | - | Chr5:75093113-75093132 | MsG0580028129.01.T01:intron | 30.0% | |
| TACTTGCATAACTTGGAATA+TGG | - | Chr5:75092853-75092872 | MsG0580028129.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 75092365 | 75094374 | 75092365 | ID=MsG0580028129.01;Name=MsG0580028129.01 |
| Chr5 | mRNA | 75092365 | 75094374 | 75092365 | ID=MsG0580028129.01.T01;Parent=MsG0580028129.01;Name=MsG0580028129.01.T01;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|1|3|0|323 |
| Chr5 | exon | 75094165 | 75094374 | 75094165 | ID=MsG0580028129.01.T01:exon:45785;Parent=MsG0580028129.01.T01 |
| Chr5 | exon | 75093163 | 75093357 | 75093163 | ID=MsG0580028129.01.T01:exon:45784;Parent=MsG0580028129.01.T01 |
| Chr5 | exon | 75092365 | 75092931 | 75092365 | ID=MsG0580028129.01.T01:exon:45783;Parent=MsG0580028129.01.T01 |
| Chr5 | CDS | 75094165 | 75094374 | 75094165 | ID=MsG0580028129.01.T01:cds;Parent=MsG0580028129.01.T01 |
| Chr5 | CDS | 75093163 | 75093357 | 75093163 | ID=MsG0580028129.01.T01:cds;Parent=MsG0580028129.01.T01 |
| Chr5 | CDS | 75092365 | 75092931 | 75092365 | ID=MsG0580028129.01.T01:cds;Parent=MsG0580028129.01.T01 |
| Gene Sequence |
>MsG0580028129.01.T01 ATGGATTCTCGTATCCAATTTGTGCTTTTTGTGGTCACTTTTGCAACAATTTTGAGTCCTACCATTGCAAAGCTGACTCCAAATTATTACGACAGAATTTGCCCTAAGGCATTGCCAATCATAAAGTCAATTGTTAAGCAAGCAATTACTCGTGAGCCACGCATGGGAGCATCGTTGCTACGTTTGCATTTCCATGACTTCTTTGTTAACGGTTGTGATGGATCGGTTCTGCTAGATGATACTCCTACCTTCATTGGTGAGAAGACTGCATTTCCAAATATCAATTCAATTAGAGGATTTGAGGTTGTTGATCAAATTAAAGAAGCAGTTACGAAAGCTTGCAAACGCGATGTAGTATCATGTGCAGATATTTTAGCAATAGCAGCTCGAGACTCCGTAGCCATATTGGGTGGTAAGCAATATTGGTACCAAGTGTTACTAGGAAGAAGAGATTCAAGATTTGCAAGCAGGGATGCAGCAAATACAAATCTTACTCCTCCATTTTTCAACTTCTCACAACTCATCACAAATTTCAAGTCACATGGAGTTAACCTTAAAGACTTAGTTGTTCTATCTGGTGGTCACACAATTGGATTTTCCCAATGCACTAACTTTAGAAACAGAATCTACAATGAAACCCACATAGACACCAATTTTGCAGCAAATTTGCAAAAGACATGCCCTAGAATCGGCGGCGATGGTAATTTAGCACCATTTGATTCTACACCTAACAAAGATGACACATCCTATTATAAGGCTTTGTTGTATAAACAAGGTCTCCTTCATTCTGATCAAGAGCTTTTTAAAGGTGATGGTTCTCAAAGTGATAAGTTGGTGCAACTATATAGTAAAAATTCATATGCTTTTGCATATGATTTTGGAGTTTCTATGATTAAGATGGGTAACTTTAAGCCTCTTACCGGGAAAAAGGGGGAGATCAGGTGCAATTGCAGAAAAGTTAACCAGATATGA |
| Protein sequence |
>MsG0580028129.01.T01 MDSRIQFVLFVVTFATILSPTIAKLTPNYYDRICPKALPIIKSIVKQAITREPRMGASLLRLHFHDFFVNGCDGSVLLDDTPTFIGEKTAFPNINSIRGFEVVDQIKEAVTKACKRDVVSCADILAIAARDSVAILGGKQYWYQVLLGRRDSRFASRDAANTNLTPPFFNFSQLITNFKSHGVNLKDLVVLSGGHTIGFSQCTNFRNRIYNETHIDTNFAANLQKTCPRIGGDGNLAPFDSTPNKDDTSYYKALLYKQGLLHSDQELFKGDGSQSDKLVQLYSKNSYAFAYDFGVSMIKMGNFKPLTGKKGEIRCNCRKVNQI* |
