Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028556.01.T01 | PNX76348.1 | 67.442 | 86 | 28 | 0 | 1 | 86 | 269 | 354 | 8.77E-33 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028556.01.T01 | A0A2K3LCT6 | 67.442 | 86 | 28 | 0 | 1 | 86 | 269 | 354 | 4.19e-33 | 127 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005457.01 | MsG0580028556.01 | 0.812277 | 4.596662e-51 | 2.910181e-48 |
| MsG0280008437.01 | MsG0580028556.01 | 0.824559 | 7.558933e-54 | 6.719104e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028556.01.T01 | MTR_6g012560 | 64.557 | 79 | 28 | 0 | 24 | 102 | 109 | 187 | 4.12e-29 | 104 |
| MsG0580028556.01.T01 | MTR_4g125750 | 81.818 | 55 | 10 | 0 | 1 | 55 | 145 | 199 | 6.99e-25 | 93.2 |
| MsG0580028556.01.T01 | MTR_2g021870 | 51.562 | 64 | 24 | 1 | 23 | 86 | 1 | 57 | 9.35e-15 | 63.9 |
| MsG0580028556.01.T01 | MTR_6g012540 | 52.459 | 61 | 26 | 1 | 39 | 99 | 169 | 226 | 2.24e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACATTTATTCCCGTGTAAA+AGG | 0.219497 | 5:+82265193 | MsG0580028556.01.T01:CDS |
| AGAGAGGACAAATGTGTTTA+TGG | 0.232453 | 5:+82265336 | MsG0580028556.01.T01:CDS |
| AGGGGACATGTGCGTTGTAT+AGG | 0.380197 | 5:+82265226 | MsG0580028556.01.T01:CDS |
| GTCTGAAAATCAAGAATTGA+GGG | 0.452871 | 5:+82265285 | MsG0580028556.01.T01:CDS |
| TGTCTGAAAATCAAGAATTG+AGG | 0.457495 | 5:+82265284 | MsG0580028556.01.T01:CDS |
| GGACACAAAGTGATATGCCT+TGG | 0.530352 | 5:+82265164 | None:intergenic |
| GTGTAAAAGGAGCTGAAAAG+AGG | 0.564728 | 5:+82265206 | MsG0580028556.01.T01:CDS |
| TGTAAAAGGAGCTGAAAAGA+GGG | 0.578637 | 5:+82265207 | MsG0580028556.01.T01:CDS |
| GAATAAATGTCATTCTTCCA+AGG | 0.592306 | 5:-82265181 | None:intergenic |
| AAATGCGAGAAAGTCAAGAG+AGG | 0.636328 | 5:+82265320 | MsG0580028556.01.T01:CDS |
| GTAAAAGGAGCTGAAAAGAG+GGG | 0.720928 | 5:+82265208 | MsG0580028556.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAAGCTTTTGATTTCTT+AGG | - | Chr5:82265265-82265284 | None:intergenic | 25.0% |
| GAATAAATGTCATTCTTCCA+AGG | - | Chr5:82265184-82265203 | None:intergenic | 30.0% | |
| GTCTGAAAATCAAGAATTGA+GGG | + | Chr5:82265285-82265304 | MsG0580028556.01.T01:CDS | 30.0% | |
| TGTCTGAAAATCAAGAATTG+AGG | + | Chr5:82265284-82265303 | MsG0580028556.01.T01:CDS | 30.0% | |
| ! | TATTTTACAAGCTGCAAAAC+AGG | + | Chr5:82265411-82265430 | MsG0580028556.01.T01:CDS | 30.0% |
| !!! | AGCTTTTGATTTCTTAGGTT+TGG | - | Chr5:82265260-82265279 | None:intergenic | 30.0% |
| !!! | GCTTTTGATTTCTTAGGTTT+GGG | - | Chr5:82265259-82265278 | None:intergenic | 30.0% |
| AGAGAGGACAAATGTGTTTA+TGG | + | Chr5:82265336-82265355 | MsG0580028556.01.T01:CDS | 35.0% | |
| GACATTTATTCCCGTGTAAA+AGG | + | Chr5:82265193-82265212 | MsG0580028556.01.T01:CDS | 35.0% | |
| TGTAAAAGGAGCTGAAAAGA+GGG | + | Chr5:82265207-82265226 | MsG0580028556.01.T01:CDS | 35.0% | |
| ! | TCTTTTCAGCTCCTTTTACA+CGG | - | Chr5:82265207-82265226 | None:intergenic | 35.0% |
| AAATGCGAGAAAGTCAAGAG+AGG | + | Chr5:82265320-82265339 | MsG0580028556.01.T01:CDS | 40.0% | |
| GTAAAAGGAGCTGAAAAGAG+GGG | + | Chr5:82265208-82265227 | MsG0580028556.01.T01:CDS | 40.0% | |
| GTGTAAAAGGAGCTGAAAAG+AGG | + | Chr5:82265206-82265225 | MsG0580028556.01.T01:CDS | 40.0% | |
| ! | CTTTTCAGCTCCTTTTACAC+GGG | - | Chr5:82265206-82265225 | None:intergenic | 40.0% |
| AGGGGACATGTGCGTTGTAT+AGG | + | Chr5:82265226-82265245 | MsG0580028556.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 82265178 | 82265486 | 82265178 | ID=MsG0580028556.01;Name=MsG0580028556.01 |
| Chr5 | mRNA | 82265178 | 82265486 | 82265178 | ID=MsG0580028556.01.T01;Parent=MsG0580028556.01;Name=MsG0580028556.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|0|1|0|102 |
| Chr5 | exon | 82265178 | 82265486 | 82265178 | ID=MsG0580028556.01.T01:exon:24852;Parent=MsG0580028556.01.T01 |
| Chr5 | CDS | 82265178 | 82265486 | 82265178 | ID=MsG0580028556.01.T01:cds;Parent=MsG0580028556.01.T01 |
| Gene Sequence |
| Protein sequence |
