AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028722.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028722.01.T01 MTR_5g076590 79.470 151 3 1 15 165 45 167 6.28e-79 234
MsG0580028722.01.T01 MTR_3g064390 72.848 151 13 1 15 165 45 167 1.98e-73 220
MsG0580028722.01.T01 MTR_3g064390 84.536 97 15 0 16 112 46 142 7.68e-58 179
MsG0580028722.01.T01 MTR_8g103550 55.629 151 39 1 15 165 44 166 8.29e-56 175
MsG0580028722.01.T01 MTR_2g102060 52.318 151 44 1 16 166 46 168 3.43e-52 166
MsG0580028722.01.T01 MTR_1g094220 52.632 152 43 2 15 165 66 189 1.11e-48 157
MsG0580028722.01.T01 MTR_1g094220 51.974 152 44 2 15 165 66 189 4.70e-48 155
MsG0580028722.01.T01 MTR_1g094220 48.235 170 55 4 16 184 67 204 9.29e-48 155
MsG0580028722.01.T01 MTR_1g094220 50.000 166 47 4 16 173 67 204 4.54e-47 154
MsG0580028722.01.T01 MTR_6g021790 36.076 158 71 2 19 176 45 172 2.54e-30 110
MsG0580028722.01.T01 MTR_8g089985 38.776 147 62 2 19 165 56 174 3.88e-30 110
MsG0580028722.01.T01 MTR_8g090215 33.742 163 80 1 18 180 47 181 1.10e-29 108
MsG0580028722.01.T01 MTR_8g031660 35.570 149 68 1 22 170 48 168 1.18e-29 108
MsG0580028722.01.T01 MTR_2g083860 36.735 147 65 2 19 165 52 170 1.50e-29 108
MsG0580028722.01.T01 MTR_3g087160 34.437 151 71 1 18 168 47 169 3.00e-29 107
MsG0580028722.01.T01 MTR_3g086980 38.776 147 62 2 19 165 55 173 4.32e-29 107
MsG0580028722.01.T01 MTR_8g027220 38.776 147 62 2 19 165 65 183 1.03e-28 107
MsG0580028722.01.T01 MTR_6g079550 37.671 146 63 1 20 165 52 169 2.54e-28 105
MsG0580028722.01.T01 MTR_2g075950 36.735 147 65 2 19 165 52 170 2.74e-28 105
MsG0580028722.01.T01 MTR_2g027660 34.211 152 72 1 19 170 45 168 3.49e-28 105
MsG0580028722.01.T01 MTR_4g099470 35.811 148 67 1 20 167 24 143 8.81e-28 103
MsG0580028722.01.T01 MTR_8g031870 34.965 143 65 1 20 162 46 160 1.49e-27 102
MsG0580028722.01.T01 MTR_4g064897 36.735 147 65 1 19 165 51 169 1.95e-27 103
MsG0580028722.01.T01 MTR_0262s0050 33.728 169 80 3 1 165 32 172 2.29e-27 103
MsG0580028722.01.T01 MTR_2g005510 38.889 144 60 1 19 162 54 169 2.70e-27 103
MsG0580028722.01.T01 MTR_4g079350 34.694 147 68 1 19 165 52 170 7.25e-27 102
MsG0580028722.01.T01 MTR_2g014990 35.374 147 67 1 19 165 52 170 1.08e-26 101
MsG0580028722.01.T01 MTR_5g013900 32.278 158 79 1 18 175 47 176 1.10e-26 101
MsG0580028722.01.T01 MTR_4g098950 32.278 158 79 1 18 175 47 176 1.26e-26 100
MsG0580028722.01.T01 MTR_3g069510 36.735 147 65 1 19 165 51 169 1.63e-26 100
MsG0580028722.01.T01 MTR_8g031680 32.500 160 80 1 20 179 46 177 2.36e-26 100
MsG0580028722.01.T01 MTR_8g036220 37.931 145 62 2 18 162 54 170 5.97e-26 99.8
MsG0580028722.01.T01 MTR_1g078200 37.143 140 60 2 19 158 53 164 6.83e-26 99.8
MsG0580028722.01.T01 MTR_1g052475 36.054 147 66 2 19 165 54 172 6.97e-26 99.4
MsG0580028722.01.T01 MTR_4g088090 34.694 147 68 1 19 165 52 170 1.46e-25 98.6
MsG0580028722.01.T01 MTR_7g051940 36.111 144 64 1 19 162 52 167 1.62e-25 98.2
MsG0580028722.01.T01 MTR_7g081700 34.228 149 70 1 20 168 48 168 3.88e-25 97.1
MsG0580028722.01.T01 MTR_2g005500 34.459 148 69 1 20 167 49 168 5.95e-25 96.7
MsG0580028722.01.T01 MTR_7g405790 34.722 144 66 1 19 162 54 169 1.51e-24 95.9
MsG0580028722.01.T01 MTR_1g068730 31.507 146 73 2 20 165 55 173 1.79e-24 95.5
MsG0580028722.01.T01 MTR_8g006970 34.228 149 70 1 20 168 48 168 2.34e-24 94.4
MsG0580028722.01.T01 MTR_2g090865 32.099 162 82 2 19 180 50 183 3.53e-24 94.7
MsG0580028722.01.T01 MTR_8g006970 34.228 149 70 1 20 168 62 182 3.80e-24 94.7
MsG0580028722.01.T01 MTR_8g031830 33.099 142 67 1 20 161 46 159 3.80e-24 94.4
MsG0580028722.01.T01 MTR_1g068740 31.507 146 73 2 20 165 55 173 4.18e-24 94.7
MsG0580028722.01.T01 MTR_7g100020 30.822 146 74 2 20 165 55 173 7.73e-24 94.0
MsG0580028722.01.T01 MTR_1g115325 30.822 146 74 2 20 165 55 173 1.26e-23 93.6
MsG0580028722.01.T01 MTR_7g117910 43.529 85 48 0 19 103 59 143 8.03e-23 91.3
MsG0580028722.01.T01 MTR_8g107070 33.125 160 79 1 20 179 54 185 2.30e-22 91.3
MsG0580028722.01.T01 MTR_1g068730 32.231 121 69 2 20 140 55 162 5.86e-21 85.5
MsG0580028722.01.T01 MTR_4g063160 30.405 148 74 3 20 165 55 175 2.26e-20 84.7
MsG0580028722.01.T01 MTR_7g103070 33.136 169 79 4 22 184 2 142 3.62e-20 82.8
MsG0580028722.01.T01 MTR_1g062760 34.932 146 65 3 22 165 55 172 4.75e-20 84.0
MsG0580028722.01.T01 MTR_1g068740 35.484 93 59 1 20 112 55 146 5.62e-20 83.6
MsG0580028722.01.T01 MTR_8g093740 33.333 165 72 4 22 184 21 149 7.15e-20 82.8
MsG0580028722.01.T01 MTR_7g103070 34.459 148 67 3 20 165 56 175 9.57e-20 83.2
MsG0580028722.01.T01 MTR_8g093740 33.750 160 68 4 22 179 54 177 2.31e-19 82.0
MsG0580028722.01.T01 MTR_3g096350 32.877 146 68 3 22 165 53 170 1.56e-18 79.7
MsG0580028722.01.T01 MTR_2g096550 27.027 148 80 3 19 165 54 174 1.77e-18 79.7
MsG0580028722.01.T01 MTR_1g029450 34.532 139 61 3 29 165 65 175 2.99e-18 79.3
MsG0580028722.01.T01 MTR_1g090717 31.507 146 70 3 22 165 58 175 1.02e-17 77.8
MsG0580028722.01.T01 MTR_8g092020 32.450 151 67 5 19 162 51 173 6.33e-17 75.5
MsG0580028722.01.T01 MTR_1g097420 31.613 155 75 4 19 168 74 202 4.15e-16 73.9
MsG0580028722.01.T01 MTR_1g097420 32.237 152 72 4 19 165 47 172 4.31e-16 73.6
MsG0580028722.01.T01 MTR_3g078260 31.507 146 81 4 18 162 45 172 7.14e-16 72.8
MsG0580028722.01.T01 MTR_8g075240 32.192 146 80 4 18 162 43 170 1.45e-15 71.6
MsG0580028722.01.T01 MTR_5g022600 31.507 146 81 4 18 162 43 170 5.16e-15 70.5
MsG0580028722.01.T01 MTR_4g069850 29.221 154 76 4 18 165 46 172 1.59e-14 69.3
MsG0580028722.01.T01 MTR_4g088055 28.395 162 91 5 3 162 32 170 1.60e-14 68.9
MsG0580028722.01.T01 MTR_1452s0010 42.857 63 36 0 19 81 20 82 2.25e-14 66.2
MsG0580028722.01.T01 MTR_2g090875 28.571 161 92 5 3 162 32 170 5.47e-14 67.4
MsG0580028722.01.T01 MTR_6g087980 31.507 146 81 4 18 162 43 170 8.71e-14 67.4
MsG0580028722.01.T01 MTR_4g015360 47.761 67 35 0 118 184 5 71 1.99e-13 64.3
MsG0580028722.01.T01 MTR_2g101890 36.471 85 50 1 18 98 48 132 2.36e-13 66.2
MsG0580028722.01.T01 MTR_4g073250 32.192 146 80 5 18 162 43 170 2.66e-13 65.9
MsG0580028722.01.T01 MTR_3g096350 37.662 77 46 2 22 96 53 129 5.55e-13 63.9
MsG0580028722.01.T01 MTR_3g096350 37.662 77 46 2 22 96 53 129 5.81e-13 63.9
MsG0580028722.01.T01 MTR_1686s0010 39.344 61 37 0 18 78 19 79 1.55e-12 61.2
MsG0580028722.01.T01 MTR_4g012940 35.294 85 51 1 18 98 46 130 1.95e-12 63.5
MsG0580028722.01.T01 MTR_3g078260 38.462 78 46 2 18 94 45 121 5.58e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028722.01.T01 AT5G45130 68.212 151 20 1 15 165 45 167 6.59e-66 201
MsG0580028722.01.T01 AT5G45130 68.212 151 20 1 15 165 53 175 1.22e-65 201
MsG0580028722.01.T01 AT4G19640 66.447 152 23 1 15 166 45 168 1.67e-65 200
MsG0580028722.01.T01 AT4G19640 77.143 105 24 0 15 119 45 149 9.89e-57 176
MsG0580028722.01.T01 AT4G19640 83.871 93 15 0 15 107 45 137 4.55e-53 168
MsG0580028722.01.T01 AT3G54840 52.941 153 43 3 15 166 34 158 6.45e-49 157
MsG0580028722.01.T01 AT3G54840 52.941 153 43 3 15 166 68 192 2.58e-48 156
MsG0580028722.01.T01 AT3G54840 52.703 148 41 3 16 162 69 188 3.60e-46 150
MsG0580028722.01.T01 AT1G16920 39.456 147 61 2 19 165 52 170 2.29e-31 113
MsG0580028722.01.T01 AT4G39990 38.889 144 60 2 19 162 56 171 1.65e-30 111
MsG0580028722.01.T01 AT4G17160 34.969 163 78 1 19 181 45 179 8.93e-30 109
MsG0580028722.01.T01 AT4G35860 36.774 155 68 2 22 176 2 126 1.11e-29 107
MsG0580028722.01.T01 AT4G35860 36.076 158 71 2 19 176 45 172 1.97e-29 108
MsG0580028722.01.T01 AT5G45750 36.735 147 65 2 19 165 52 170 3.96e-29 107
MsG0580028722.01.T01 AT4G18800 36.054 147 66 2 19 165 52 170 5.61e-29 107
MsG0580028722.01.T01 AT2G44610 35.443 158 74 1 18 175 47 176 7.13e-29 107
MsG0580028722.01.T01 AT5G65270 37.415 147 64 2 19 165 56 174 8.20e-29 107
MsG0580028722.01.T01 AT5G60860 34.161 161 78 1 19 179 52 184 9.34e-29 107
MsG0580028722.01.T01 AT5G10260 34.177 158 76 1 18 175 47 176 9.49e-29 106
MsG0580028722.01.T01 AT1G06400 37.415 147 64 2 19 165 52 170 1.38e-28 106
MsG0580028722.01.T01 AT1G07410 38.095 147 63 1 19 165 51 169 5.95e-28 104
MsG0580028722.01.T01 AT4G17170 34.211 152 72 1 19 170 45 168 6.79e-28 104
MsG0580028722.01.T01 AT4G18430 33.129 163 81 2 19 181 52 186 8.08e-28 104
MsG0580028722.01.T01 AT5G59150 37.500 152 67 1 19 170 51 174 1.25e-27 103
MsG0580028722.01.T01 AT1G28550 36.054 147 66 2 19 165 52 170 1.27e-27 103
MsG0580028722.01.T01 AT4G39890 33.553 152 72 2 18 168 47 170 1.37e-27 103
MsG0580028722.01.T01 AT1G09630 38.776 147 62 1 19 165 51 169 1.71e-27 103
MsG0580028722.01.T01 AT3G12160 38.095 147 63 2 19 165 54 172 1.78e-27 103
MsG0580028722.01.T01 AT3G15060 33.540 161 79 1 19 179 52 184 2.40e-27 103
MsG0580028722.01.T01 AT3G07410 38.889 144 60 1 19 162 51 166 4.80e-27 102
MsG0580028722.01.T01 AT2G22290 33.775 151 72 1 18 168 47 169 6.41e-27 101
MsG0580028722.01.T01 AT2G33870 34.161 161 78 2 19 179 52 184 6.76e-27 102
MsG0580028722.01.T01 AT5G59150 36.913 149 66 1 22 170 3 123 1.15e-26 100
MsG0580028722.01.T01 AT3G46830 36.735 147 65 1 19 165 51 169 1.71e-26 100
MsG0580028722.01.T01 AT3G46830 36.735 147 65 1 19 165 51 169 1.71e-26 100
MsG0580028722.01.T01 AT4G17530 34.228 149 70 1 20 168 48 168 5.47e-26 99.4
MsG0580028722.01.T01 AT1G02130 35.570 149 68 1 20 168 48 168 9.86e-26 98.6
MsG0580028722.01.T01 AT5G47960 36.054 147 66 2 19 165 54 172 1.07e-25 99.0
MsG0580028722.01.T01 AT5G47200 34.228 149 70 1 20 168 48 168 1.31e-25 98.2
MsG0580028722.01.T01 AT1G18200 36.025 161 74 2 20 179 53 185 2.51e-25 98.2
MsG0580028722.01.T01 AT1G73640 36.364 143 63 1 20 162 53 167 3.04e-25 98.2
MsG0580028722.01.T01 AT1G05810 35.417 144 65 1 19 162 51 166 4.11e-25 97.4
MsG0580028722.01.T01 AT5G47520 34.722 144 66 1 19 162 53 168 4.78e-25 97.4
MsG0580028722.01.T01 AT1G05810 35.417 144 65 1 19 162 97 212 6.15e-25 98.2
MsG0580028722.01.T01 AT3G46060 31.293 147 74 2 20 166 55 174 1.98e-24 95.5
MsG0580028722.01.T01 AT3G46060 31.293 147 74 2 20 166 55 174 1.98e-24 95.5
MsG0580028722.01.T01 AT3G46060 31.293 147 74 2 20 166 55 174 1.98e-24 95.5
MsG0580028722.01.T01 AT5G64990 33.108 148 71 1 18 165 45 164 4.93e-24 94.4
MsG0580028722.01.T01 AT2G43130 34.722 144 66 1 19 162 51 166 6.01e-24 94.4
MsG0580028722.01.T01 AT3G11730 33.784 148 70 1 20 167 48 167 6.03e-24 94.0
MsG0580028722.01.T01 AT1G01200 34.694 147 69 2 19 165 67 186 9.43e-24 94.4
MsG0580028722.01.T01 AT2G31680 34.028 144 67 1 19 162 51 166 1.58e-23 93.2
MsG0580028722.01.T01 AT5G59840 30.137 146 75 2 20 165 55 173 4.55e-23 92.0
MsG0580028722.01.T01 AT5G03520 30.612 147 75 2 20 166 45 164 1.29e-22 90.5
MsG0580028722.01.T01 AT5G03520 30.822 146 74 2 20 165 55 173 1.96e-22 90.5
MsG0580028722.01.T01 AT3G53610 29.452 146 76 2 20 165 55 173 2.07e-22 90.5
MsG0580028722.01.T01 AT3G53610 29.452 146 76 2 20 165 55 173 2.07e-22 90.5
MsG0580028722.01.T01 AT3G53610 29.452 146 76 2 20 165 55 173 2.07e-22 90.5
MsG0580028722.01.T01 AT3G09900 30.137 146 75 2 20 165 55 173 1.31e-21 88.2
MsG0580028722.01.T01 AT5G64990 31.613 155 71 2 18 165 45 171 2.20e-21 87.4
MsG0580028722.01.T01 AT1G43890 32.877 146 68 3 22 165 54 171 3.58e-19 81.6
MsG0580028722.01.T01 AT1G43890 32.877 146 68 3 22 165 54 171 3.58e-19 81.6
MsG0580028722.01.T01 AT1G43890 32.877 146 68 3 22 165 54 171 3.58e-19 81.6
MsG0580028722.01.T01 AT5G03530 33.784 148 68 3 20 165 52 171 1.30e-18 80.1
MsG0580028722.01.T01 AT5G03530 33.784 148 68 3 20 165 52 171 1.30e-18 80.1
MsG0580028722.01.T01 AT1G22740 32.026 153 71 4 19 165 47 172 1.14e-17 77.4
MsG0580028722.01.T01 AT3G09910 31.081 148 72 3 20 165 52 171 3.03e-17 76.6
MsG0580028722.01.T01 AT3G09910 31.081 148 72 3 20 165 52 171 3.03e-17 76.6
MsG0580028722.01.T01 AT3G09910 31.081 148 72 3 20 165 52 171 3.03e-17 76.6
MsG0580028722.01.T01 AT2G21880 41.667 84 45 1 19 98 23 106 3.85e-17 75.9
MsG0580028722.01.T01 AT2G21880 41.667 84 45 1 19 98 23 106 3.85e-17 75.9
MsG0580028722.01.T01 AT2G21880 41.667 84 45 1 19 98 48 131 5.56e-17 75.9
MsG0580028722.01.T01 AT3G09910 31.081 148 72 3 20 165 52 171 6.24e-17 75.5
MsG0580028722.01.T01 AT3G09910 31.081 148 72 3 20 165 52 171 6.24e-17 75.5
MsG0580028722.01.T01 AT4G35950 35.246 122 73 4 18 135 43 162 6.03e-16 72.8
MsG0580028722.01.T01 AT2G17800 33.557 149 74 5 18 162 43 170 6.69e-16 72.8
MsG0580028722.01.T01 AT2G17800 33.557 149 74 5 18 162 43 170 6.69e-16 72.8
MsG0580028722.01.T01 AT3G51300 32.192 146 80 4 18 162 43 170 7.51e-16 72.4
MsG0580028722.01.T01 AT2G44690 32.237 152 76 6 18 164 55 184 9.76e-16 72.4
MsG0580028722.01.T01 AT4G35020 32.886 149 75 5 18 162 43 170 3.71e-15 70.9
MsG0580028722.01.T01 AT4G35020 32.886 149 75 5 18 162 43 170 3.71e-15 70.9
MsG0580028722.01.T01 AT4G35020 32.886 149 75 5 18 162 43 170 3.71e-15 70.9
MsG0580028722.01.T01 AT5G39620 27.976 168 91 4 16 179 41 182 5.72e-15 70.5
MsG0580028722.01.T01 AT3G18820 30.070 143 67 4 26 162 54 169 7.82e-15 70.1
MsG0580028722.01.T01 AT3G51290 32.192 146 80 4 18 162 644 771 1.24e-14 71.6
MsG0580028722.01.T01 AT4G09720 39.286 84 47 1 19 98 13 96 1.40e-14 68.6
MsG0580028722.01.T01 AT4G09720 39.286 84 47 1 19 98 13 96 1.40e-14 68.6
MsG0580028722.01.T01 AT4G09720 39.286 84 47 1 19 98 13 96 1.40e-14 68.6
MsG0580028722.01.T01 AT4G09720 39.286 84 47 1 19 98 47 130 2.19e-14 68.9
MsG0580028722.01.T01 AT1G52280 28.302 159 81 4 18 170 46 177 2.67e-14 68.6
MsG0580028722.01.T01 AT1G20090 31.507 146 81 4 18 162 42 169 2.85e-14 68.2
MsG0580028722.01.T01 AT1G75840 31.507 146 81 4 18 162 43 170 2.93e-14 68.2
MsG0580028722.01.T01 AT3G48040 30.137 146 83 4 18 162 45 172 3.25e-14 68.6
MsG0580028722.01.T01 AT5G55080 26.946 167 106 6 16 172 49 209 3.59e-14 68.6
MsG0580028722.01.T01 AT1G49300 30.769 143 66 4 26 162 54 169 6.67e-14 67.4
MsG0580028722.01.T01 AT1G49300 30.769 143 66 4 26 162 54 169 6.67e-14 67.4
MsG0580028722.01.T01 AT5G45970 29.012 162 90 5 3 162 32 170 7.97e-14 67.4
MsG0580028722.01.T01 AT5G62880 30.137 146 83 4 18 162 45 172 1.33e-13 67.0
MsG0580028722.01.T01 AT4G28950 30.137 146 81 4 18 162 43 168 3.15e-13 65.9
MsG0580028722.01.T01 AT3G16100 34.043 94 57 2 18 106 46 139 1.08e-12 64.3
MsG0580028722.01.T01 AT3G48040 37.647 85 51 2 18 101 45 128 1.27e-12 63.5
MsG0580028722.01.T01 AT4G09720 42.857 63 36 0 19 81 47 109 3.32e-12 63.2
MsG0580028722.01.T01 AT5G62880 35.556 90 56 2 18 106 45 133 4.17e-12 62.4
MsG0580028722.01.T01 AT4G09720 42.857 63 36 0 19 81 47 109 4.59e-12 62.8

Find 42 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATACGCCAGTTACATTTAT+TGG 0.106564 5:+85356378 MsG0580028722.01.T01:CDS
CCACAGTGAAATTTGAGATT+TGG 0.262162 5:+85354300 MsG0580028722.01.T01:CDS
CACAGTGAAATTTGAGATTT+GGG 0.299158 5:+85354301 MsG0580028722.01.T01:CDS
CGTCATTTACTGCCAACGTC+TGG 0.316274 5:-85354277 None:intergenic
GTCTTTACCCTCCGGTTCCC+AGG 0.343615 5:-85354029 None:intergenic
TTACCATGGGGCCTTTCCCC+TGG 0.350595 5:+85354011 None:intergenic
GGGGCCTTTCCCCTGGGAAC+CGG 0.380047 5:+85354018 MsG0580028722.01.T01:CDS
CATTTACACGAGCGAAGAAA+TGG 0.391385 5:+85354622 MsG0580028722.01.T01:CDS
GTAGTTCCAATAAATGTAAC+TGG 0.409678 5:-85356384 None:intergenic
CGTGTATATGCTGAGGAAAA+TGG 0.414206 5:+85356448 MsG0580028722.01.T01:CDS
GCAAAAGCCACGACCATGTT+CGG 0.437301 5:-85355193 None:intergenic
TACCTTGCTTCTGAAGCTCT+TGG 0.443949 5:-85354647 None:intergenic
TGAAAACAGGAATCAACAAT+AGG 0.444976 5:+85354240 MsG0580028722.01.T01:intron
GCCTTTCCCCTGGGAACCGG+AGG 0.464249 5:+85354021 MsG0580028722.01.T01:CDS
TGTCTATGATATTACTAACT+TGG 0.471350 5:+85354394 MsG0580028722.01.T01:CDS
TGCTGGTAACAAAGCTGACT+TGG 0.472663 5:+85355213 MsG0580028722.01.T01:CDS
TACCATGGGGCCTTTCCCCT+GGG 0.473105 5:+85354012 None:intergenic
ATAAAATATGTCATTAACAT+TGG 0.476631 5:-85356506 None:intergenic
GAGTCATGAGCTGGATAAGT+TGG 0.498525 5:-85356406 None:intergenic
TGAAGTGTATGTAATAGAAA+AGG 0.502053 5:-85356544 None:intergenic
CCCTCCGGTTCCCAGGGGAA+AGG 0.503338 5:-85354022 None:intergenic
AAGCTGACTTGGAAGATAAG+AGG 0.510951 5:+85355224 MsG0580028722.01.T01:CDS
TCTTTACCCTCCGGTTCCCA+GGG 0.516430 5:-85354028 None:intergenic
TCATGACTCTTGTACAATGC+AGG 0.516982 5:+85356420 MsG0580028722.01.T01:CDS
AGTAATATCATAGACAATGA+TGG 0.531370 5:-85354387 None:intergenic
GTCATTTACTGCCAACGTCT+GGG 0.532033 5:-85354276 None:intergenic
TTAGCTCCCATGTACTATAG+AGG 0.537219 5:+85354353 MsG0580028722.01.T01:CDS
TTTGAGATTTGGGACACAGC+AGG 0.538081 5:+85354311 MsG0580028722.01.T01:CDS
AATAAATGTAACTGGCGTAT+CGG 0.549137 5:-85356376 None:intergenic
ATTTACACGAGCGAAGAAAT+GGG 0.562436 5:+85354623 MsG0580028722.01.T01:CDS
AGCAGCGCCTCTATAGTACA+TGG 0.565513 5:-85354360 None:intergenic
AGGGAATCCGAACATGGTCG+TGG 0.578957 5:+85355186 MsG0580028722.01.T01:intron
TGTTGCAGGGAATCCGAACA+TGG 0.580245 5:+85355180 MsG0580028722.01.T01:intron
TTCCCAGGGGAAAGGCCCCA+TGG 0.589240 5:-85354014 None:intergenic
CCTTTCCCCTGGGAACCGGA+GGG 0.589485 5:+85354022 MsG0580028722.01.T01:CDS
AGTACATGGGAGCTAAGCTA+TGG 0.598496 5:-85354346 None:intergenic
GTCCAAGAGCTTCAGAAGCA+AGG 0.599811 5:+85354645 MsG0580028722.01.T01:CDS
ATTGTACAAGAGTCATGAGC+TGG 0.619001 5:-85356415 None:intergenic
GGAAGCACGTGTATATGCTG+AGG 0.658380 5:+85356441 MsG0580028722.01.T01:CDS
CTTTACCCTCCGGTTCCCAG+GGG 0.687665 5:-85354027 None:intergenic
GCAGCGCCTCTATAGTACAT+GGG 0.698335 5:-85354359 None:intergenic
CCAAATCTCAAATTTCACTG+TGG 0.747675 5:-85354300 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATAGATTGTAAAA+AGG + Chr5:85354723-85354742 MsG0580028722.01.T01:intron 10.0%
!! AACTAAGAAATATTAGAATA+TGG - Chr5:85354811-85354830 None:intergenic 15.0%
!! ATAAAATATGTCATTAACAT+TGG - Chr5:85356509-85356528 None:intergenic 15.0%
!! ATTAATATATGAATTGTGAA+CGG + Chr5:85354942-85354961 MsG0580028722.01.T01:intron 15.0%
!!! AAACAATAGCTAAAAATTTT+CGG - Chr5:85355058-85355077 None:intergenic 15.0%
!!! AATGACATATTTTATGAAAT+AGG + Chr5:85356514-85356533 MsG0580028722.01.T01:CDS 15.0%
!!! TCTAGATGATTTTTTTTTTT+TGG - Chr5:85354054-85354073 None:intergenic 15.0%
!! AGTCTATATGTTTAAAGAAT+TGG - Chr5:85354178-85354197 None:intergenic 20.0%
!! TTATAGTTTCTTCAACTATT+TGG + Chr5:85355305-85355324 MsG0580028722.01.T01:intron 20.0%
!!! CTAAAATAACCTTTTCAATA+AGG - Chr5:85355967-85355986 None:intergenic 20.0%
!!! GTTTATTTAGTTTATTACTG+TGG + Chr5:85354559-85354578 MsG0580028722.01.T01:intron 20.0%
!!! TATTTTGTATATACTAGTGT+GGG + Chr5:85354114-85354133 MsG0580028722.01.T01:intron 20.0%
!!! TTATTTTGTATATACTAGTG+TGG + Chr5:85354113-85354132 MsG0580028722.01.T01:intron 20.0%
!!! TTCTTATTCTCACTTTTTTT+TGG + Chr5:85354692-85354711 MsG0580028722.01.T01:intron 20.0%
!!! TTTTCTTTTCCTTATTGAAA+AGG + Chr5:85355955-85355974 MsG0580028722.01.T01:intron 20.0%
! AACTAACCCATGATATTTAA+TGG + Chr5:85355029-85355048 MsG0580028722.01.T01:intron 25.0%
! AAGAGCTTCTTAAGAAATTA+AGG + Chr5:85355852-85355871 MsG0580028722.01.T01:intron 25.0%
! AATTTAGCAGAAAAAATGTG+TGG - Chr5:85355352-85355371 None:intergenic 25.0%
! AGTAATATCATAGACAATGA+TGG - Chr5:85354390-85354409 None:intergenic 25.0%
! ATAAACTCCTCGAATAAAAT+GGG + Chr5:85356061-85356080 MsG0580028722.01.T01:intron 25.0%
! CTAAAATAATGAACTCATTC+TGG + Chr5:85355154-85355173 MsG0580028722.01.T01:intron 25.0%
! GCAACTGTTTAAATACATAT+AGG - Chr5:85356342-85356361 None:intergenic 25.0%
! TATAAACTCCTCGAATAAAA+TGG + Chr5:85356060-85356079 MsG0580028722.01.T01:intron 25.0%
! TCTTTAAACATATAGACTTG+TGG + Chr5:85354180-85354199 MsG0580028722.01.T01:intron 25.0%
! TGAAGTGTATGTAATAGAAA+AGG - Chr5:85356547-85356566 None:intergenic 25.0%
! TGTCTATGATATTACTAACT+TGG + Chr5:85354394-85354413 MsG0580028722.01.T01:CDS 25.0%
! TTATAAGAGAAGCTATTAGT+TGG + Chr5:85355894-85355913 MsG0580028722.01.T01:intron 25.0%
!! GTTTTCTATACATGTTACAA+AGG + Chr5:85355074-85355093 MsG0580028722.01.T01:intron 25.0%
!! TGCTAAATTTTCAAATACCA+GGG + Chr5:85355363-85355382 MsG0580028722.01.T01:intron 25.0%
!! TTTTCTATTACATACACTTC+AGG + Chr5:85356546-85356565 MsG0580028722.01.T01:CDS 25.0%
!!! ATGGGTTAGTTTCAAATTAA+CGG - Chr5:85355020-85355039 None:intergenic 25.0%
AAATTAACGGAAAGTTCAGT+AGG - Chr5:85355007-85355026 None:intergenic 30.0%
AATAAATGTAACTGGCGTAT+CGG - Chr5:85356379-85356398 None:intergenic 30.0%
AATATAGCATAGCACAAACA+TGG + Chr5:85354889-85354908 MsG0580028722.01.T01:intron 30.0%
ACACTAAAATATTCCAAGCA+TGG - Chr5:85354532-85354551 None:intergenic 30.0%
ATACACTTACTATGTCACAA+GGG - Chr5:85355812-85355831 None:intergenic 30.0%
CGGTAACCATTAAATATCAT+GGG - Chr5:85355038-85355057 None:intergenic 30.0%
GACATAGTAAGTGTATAAGT+GGG + Chr5:85355816-85355835 MsG0580028722.01.T01:intron 30.0%
GTAGTTCCAATAAATGTAAC+TGG - Chr5:85356387-85356406 None:intergenic 30.0%
TATACACTTACTATGTCACA+AGG - Chr5:85355813-85355832 None:intergenic 30.0%
TATATGAATTGTGAACGGTT+AGG + Chr5:85354947-85354966 MsG0580028722.01.T01:intron 30.0%
TCGGTAACCATTAAATATCA+TGG - Chr5:85355039-85355058 None:intergenic 30.0%
TGAAAACAGGAATCAACAAT+AGG + Chr5:85354240-85354259 MsG0580028722.01.T01:intron 30.0%
TGACATAGTAAGTGTATAAG+TGG + Chr5:85355815-85355834 MsG0580028722.01.T01:intron 30.0%
TTCAATGTACAAGCTAAAGT+CGG - Chr5:85356199-85356218 None:intergenic 30.0%
! AAATTTTCAAATACCAGGGA+TGG + Chr5:85355367-85355386 MsG0580028722.01.T01:intron 30.0%
! AATTGTACTTCAAGTTCTAG+TGG + Chr5:85355112-85355131 MsG0580028722.01.T01:intron 30.0%
! AGCTTATGCGATTTTCATTT+TGG - Chr5:85356147-85356166 None:intergenic 30.0%
! CACAGTGAAATTTGAGATTT+GGG + Chr5:85354301-85354320 MsG0580028722.01.T01:CDS 30.0%
! CACTGCTTTACTGAATATTT+TGG + Chr5:85354210-85354229 MsG0580028722.01.T01:intron 30.0%
! CTGCTAAATTTTCAAATACC+AGG + Chr5:85355362-85355381 MsG0580028722.01.T01:intron 30.0%
! TGATTATTTCTTCTGTTCAC+AGG + Chr5:85354596-85354615 MsG0580028722.01.T01:intron 30.0%
! TTTTGGTCTATTGTGAAAAC+AGG + Chr5:85354227-85354246 MsG0580028722.01.T01:intron 30.0%
!! CATGTTGATAGTTCTACTAT+CGG + Chr5:85356002-85356021 MsG0580028722.01.T01:intron 30.0%
!!! CTGTCATTTTCCTTTCTTTA+GGG + Chr5:85354491-85354510 MsG0580028722.01.T01:intron 30.0%
!!! TCTGTCATTTTCCTTTCTTT+AGG + Chr5:85354490-85354509 MsG0580028722.01.T01:intron 30.0%
AACATATAGACTTGTGGTTG+TGG + Chr5:85354186-85354205 MsG0580028722.01.T01:intron 35.0%
AATCCTTTGTACAACCTGAT+TGG + Chr5:85355543-85355562 MsG0580028722.01.T01:intron 35.0%
ACATATAGACTTGTGGTTGT+GGG + Chr5:85354187-85354206 MsG0580028722.01.T01:intron 35.0%
ACTTGAAGATGCTCAGATAT+AGG - Chr5:85355597-85355616 None:intergenic 35.0%
AGTGTATAAGTGGGATTTGT+TGG + Chr5:85355825-85355844 MsG0580028722.01.T01:intron 35.0%
ATCCTTTGTACAACCTGATT+GGG + Chr5:85355544-85355563 MsG0580028722.01.T01:intron 35.0%
ATGTTACAAAGGACTCATTC+TGG + Chr5:85355085-85355104 MsG0580028722.01.T01:intron 35.0%
ATTTACACGAGCGAAGAAAT+GGG + Chr5:85354623-85354642 MsG0580028722.01.T01:CDS 35.0%
CCAAATCTCAAATTTCACTG+TGG - Chr5:85354303-85354322 None:intergenic 35.0%
GATACGCCAGTTACATTTAT+TGG + Chr5:85356378-85356397 MsG0580028722.01.T01:CDS 35.0%
TAGAACTAAATGTCCCAATC+AGG - Chr5:85355560-85355579 None:intergenic 35.0%
TCAGTTCACTATAGCATCAT+AGG + Chr5:85355280-85355299 MsG0580028722.01.T01:intron 35.0%
TGGTCAAGATTGCTTTACAT+AGG - Chr5:85354791-85354810 None:intergenic 35.0%
TTATCAAATGAAACCATCCC+TGG - Chr5:85355383-85355402 None:intergenic 35.0%
! ACTTAAAGCATCTTCTGTGT+TGG - Chr5:85356271-85356290 None:intergenic 35.0%
! ACTTTTATGCTGCTACGTTT+TGG + Chr5:85355402-85355421 MsG0580028722.01.T01:intron 35.0%
! CCACAGTGAAATTTGAGATT+TGG + Chr5:85354300-85354319 MsG0580028722.01.T01:CDS 35.0%
! CTTTTATGCTGCTACGTTTT+GGG + Chr5:85355403-85355422 MsG0580028722.01.T01:intron 35.0%
! TAATCCACCCATTTTATTCG+AGG - Chr5:85356071-85356090 None:intergenic 35.0%
! TTCTGGGTTAGACTAACAAA+TGG + Chr5:85356028-85356047 MsG0580028722.01.T01:intron 35.0%
!! GAAATGGATTACTAGGATCT+AGG - Chr5:85356249-85356268 None:intergenic 35.0%
!! GAAGCAAGGTATTTTCTGAT+TGG + Chr5:85354659-85354678 MsG0580028722.01.T01:intron 35.0%
!! TGGGTGGATTAATTCTTTGT+TGG + Chr5:85356080-85356099 MsG0580028722.01.T01:intron 35.0%
!! TGTGTTGGAAATGGATTACT+AGG - Chr5:85356256-85356275 None:intergenic 35.0%
!!! TTTTTTTGGTCTTTACCCTC+CGG - Chr5:85354040-85354059 None:intergenic 35.0%
AACTCCTCGAATAAAATGGG+TGG + Chr5:85356064-85356083 MsG0580028722.01.T01:intron 40.0%
AAGCTCAACTTGAAGAAGCT+CGG - Chr5:85355678-85355697 None:intergenic 40.0%
AATATTCCAAGCATGGTCTC+TGG - Chr5:85354525-85354544 None:intergenic 40.0%
ACTTGAAGAAGCTCGGATAT+AGG - Chr5:85355671-85355690 None:intergenic 40.0%
AGCATCTTCTGTGTTGGAAA+TGG - Chr5:85356265-85356284 None:intergenic 40.0%
ATACACAGAGCTATAGAGAC+TGG - Chr5:85355447-85355466 None:intergenic 40.0%
ATTGTACAAGAGTCATGAGC+TGG - Chr5:85356418-85356437 None:intergenic 40.0%
CATTTACACGAGCGAAGAAA+TGG + Chr5:85354622-85354641 MsG0580028722.01.T01:CDS 40.0%
CGTGTATATGCTGAGGAAAA+TGG + Chr5:85356448-85356467 MsG0580028722.01.T01:CDS 40.0%
CTAGAAGTTCGTCCAAAATG+AGG - Chr5:85355741-85355760 None:intergenic 40.0%
CTTAAGAAATTAAGGCGCAG+AGG + Chr5:85355860-85355879 MsG0580028722.01.T01:intron 40.0%
TACACAGAGCTATAGAGACT+GGG - Chr5:85355446-85355465 None:intergenic 40.0%
TCATGACTCTTGTACAATGC+AGG + Chr5:85356420-85356439 MsG0580028722.01.T01:CDS 40.0%
TTAGCTCCCATGTACTATAG+AGG + Chr5:85354353-85354372 MsG0580028722.01.T01:CDS 40.0%
! ACTTGTTTCGAGCCTCATTT+TGG + Chr5:85355726-85355745 MsG0580028722.01.T01:intron 40.0%
! CTAGAGTATCACTACTTACG+AGG + Chr5:85354069-85354088 MsG0580028722.01.T01:intron 40.0%
! TCTTCCTTGTTTGAAGATGC+TGG - Chr5:85355495-85355514 None:intergenic 40.0%
! TGGTTGGTTACGGTTAGAAA+AGG - Chr5:85355475-85355494 None:intergenic 40.0%
! TTGAAGATGCTGGTTGGTTA+CGG - Chr5:85355485-85355504 None:intergenic 40.0%
!! AAGCTGACTTGGAAGATAAG+AGG + Chr5:85355224-85355243 MsG0580028722.01.T01:CDS 40.0%
!! TTGGACGAACTTCTAGCTTA+CGG + Chr5:85355745-85355764 MsG0580028722.01.T01:intron 40.0%
!!! ACTCATTCTGGTTTTGTTGC+AGG + Chr5:85355166-85355185 MsG0580028722.01.T01:intron 40.0%
!!! CTCATTCTGGTTTTGTTGCA+GGG + Chr5:85355167-85355186 MsG0580028722.01.T01:intron 40.0%
AGTACATGGGAGCTAAGCTA+TGG - Chr5:85354349-85354368 None:intergenic 45.0%
CCAACCAGCATCTTCAAACA+AGG + Chr5:85355488-85355507 MsG0580028722.01.T01:intron 45.0%
CGAATCAAAGCTGCAATGCA+TGG - Chr5:85356111-85356130 None:intergenic 45.0%
GAGTCATGAGCTGGATAAGT+TGG - Chr5:85356409-85356428 None:intergenic 45.0%
GTCATTTACTGCCAACGTCT+GGG - Chr5:85354279-85354298 None:intergenic 45.0%
GTTAGGCAGATTCGACTTCA+AGG + Chr5:85354964-85354983 MsG0580028722.01.T01:intron 45.0%
TACTAGTGTGGGTGCATCAA+TGG + Chr5:85354125-85354144 MsG0580028722.01.T01:intron 45.0%
TGCTGGTAACAAAGCTGACT+TGG + Chr5:85355213-85355232 MsG0580028722.01.T01:CDS 45.0%
! AGTTCTACTATCGGTGCTTC+TGG + Chr5:85356011-85356030 MsG0580028722.01.T01:intron 45.0%
! CCTTGTTTGAAGATGCTGGT+TGG - Chr5:85355491-85355510 None:intergenic 45.0%
! GTCCCAATCAGGTTGTACAA+AGG - Chr5:85355549-85355568 None:intergenic 45.0%
! GTGGGTGCATCAATGGTTTT+GGG + Chr5:85354132-85354151 MsG0580028722.01.T01:intron 45.0%
! GTTCTACTATCGGTGCTTCT+GGG + Chr5:85356012-85356031 MsG0580028722.01.T01:intron 45.0%
! TGTCACAAGGGCTTTTCAGT+TGG - Chr5:85355800-85355819 None:intergenic 45.0%
! TGTGGGTGCATCAATGGTTT+TGG + Chr5:85354131-85354150 MsG0580028722.01.T01:intron 45.0%
! TTTGAGATTTGGGACACAGC+AGG + Chr5:85354311-85354330 MsG0580028722.01.T01:CDS 45.0%
!! TACCTTGCTTCTGAAGCTCT+TGG - Chr5:85354650-85354669 None:intergenic 45.0%
!! TTATGCTGCTACGTTTTGGG+TGG + Chr5:85355406-85355425 MsG0580028722.01.T01:intron 45.0%
!!! TTTTCCTTTCTTTAGGGCCC+TGG + Chr5:85354497-85354516 MsG0580028722.01.T01:intron 45.0%
AGCAGCGCCTCTATAGTACA+TGG - Chr5:85354363-85354382 None:intergenic 50.0%
CGTCATTTACTGCCAACGTC+TGG - Chr5:85354280-85354299 None:intergenic 50.0%
GCAAAAGCCACGACCATGTT+CGG - Chr5:85355196-85355215 None:intergenic 50.0%
GCAGCGCCTCTATAGTACAT+GGG - Chr5:85354362-85354381 None:intergenic 50.0%
GTCCAAGAGCTTCAGAAGCA+AGG + Chr5:85354645-85354664 MsG0580028722.01.T01:CDS 50.0%
TGTTGCAGGGAATCCGAACA+TGG + Chr5:85355180-85355199 MsG0580028722.01.T01:intron 50.0%
! AACATGGTCGTGGCTTTTGC+TGG + Chr5:85355196-85355215 MsG0580028722.01.T01:CDS 50.0%
! CAGCATTTTCTCCCAGACGT+TGG + Chr5:85354265-85354284 MsG0580028722.01.T01:CDS 50.0%
! GGAAGCACGTGTATATGCTG+AGG + Chr5:85356441-85356460 MsG0580028722.01.T01:CDS 50.0%
AGGGAATCCGAACATGGTCG+TGG + Chr5:85355186-85355205 MsG0580028722.01.T01:intron 55.0%
TCTTTACCCTCCGGTTCCCA+GGG - Chr5:85354031-85354050 None:intergenic 55.0%
!! AAGCATGGTCTCTGGAGCCA+GGG - Chr5:85354517-85354536 None:intergenic 55.0%
CTGGCTCCAGAGACCATGCT+TGG + Chr5:85354516-85354535 MsG0580028722.01.T01:intron 60.0%
CTTTACCCTCCGGTTCCCAG+GGG - Chr5:85354030-85354049 None:intergenic 60.0%
GGAGCCAGGGCCCTAAAGAA+AGG - Chr5:85354504-85354523 None:intergenic 60.0%
GTCTTTACCCTCCGGTTCCC+AGG - Chr5:85354032-85354051 None:intergenic 60.0%
!! CAAGCATGGTCTCTGGAGCC+AGG - Chr5:85354518-85354537 None:intergenic 60.0%
CCTTTCCCCTGGGAACCGGA+GGG + Chr5:85354022-85354041 MsG0580028722.01.T01:CDS 65.0%
CCCTCCGGTTCCCAGGGGAA+AGG - Chr5:85354025-85354044 None:intergenic 70.0%
GCCTTTCCCCTGGGAACCGG+AGG + Chr5:85354021-85354040 MsG0580028722.01.T01:CDS 70.0%
GGGGCCTTTCCCCTGGGAAC+CGG + Chr5:85354018-85354037 MsG0580028722.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 85354016 85356609 85354016 ID=MsG0580028722.01;Name=MsG0580028722.01
Chr5 mRNA 85354016 85356609 85354016 ID=MsG0580028722.01.T01;Parent=MsG0580028722.01;Name=MsG0580028722.01.T01;_AED=0.44;_eAED=0.62;_QI=0|0.5|0.6|1|1|1|5|0|189
Chr5 exon 85354016 85354043 85354016 ID=MsG0580028722.01.T01:exon:5252;Parent=MsG0580028722.01.T01
Chr5 exon 85354249 85354415 85354249 ID=MsG0580028722.01.T01:exon:5253;Parent=MsG0580028722.01.T01
Chr5 exon 85354618 85354666 85354618 ID=MsG0580028722.01.T01:exon:5254;Parent=MsG0580028722.01.T01
Chr5 exon 85355188 85355261 85355188 ID=MsG0580028722.01.T01:exon:5255;Parent=MsG0580028722.01.T01
Chr5 exon 85356358 85356609 85356358 ID=MsG0580028722.01.T01:exon:5256;Parent=MsG0580028722.01.T01
Chr5 CDS 85354016 85354043 85354016 ID=MsG0580028722.01.T01:cds;Parent=MsG0580028722.01.T01
Chr5 CDS 85354249 85354415 85354249 ID=MsG0580028722.01.T01:cds;Parent=MsG0580028722.01.T01
Chr5 CDS 85354618 85354666 85354618 ID=MsG0580028722.01.T01:cds;Parent=MsG0580028722.01.T01
Chr5 CDS 85355188 85355261 85355188 ID=MsG0580028722.01.T01:cds;Parent=MsG0580028722.01.T01
Chr5 CDS 85356358 85356609 85356358 ID=MsG0580028722.01.T01:cds;Parent=MsG0580028722.01.T01
Gene Sequence

>MsG0580028722.01.T01

ATGGGGCCTTTCCCCTGGGAACCGGAGGGAATCAACAATAGGTGCAGCATTTTCTCCCAGACGTTGGCAGTAAATGACGCCACAGTGAAATTTGAGATTTGGGACACAGCAGGACAAGAGAGATACCATAGCTTAGCTCCCATGTACTATAGAGGCGCTGCTGCTGCCATCATTGTCTATGATATTACTAACTTGGAGTCATTTACACGAGCGAAGAAATGGGTCCAAGAGCTTCAGAAGCAAGGGAATCCGAACATGGTCGTGGCTTTTGCTGGTAACAAAGCTGACTTGGAAGATAAGAGGAAAGTGACAGCTGAATTGCATATTTTATACATACCGATACGCCAGTTACATTTATTGGAACTACCAACTTATCCAGCTCATGACTCTTGTACAATGCAGGAAGCACGTGTATATGCTGAGGAAAATGGTCTGTTTTTCATAGAGACATCTGCGAAAACTGCAGCCAATGTTAATGACATATTTTATGAAATAGGTGCGAGTCCTTTTCTATTACATACACTTCAGGTGCTTGACAAGTCGTTTATTTTATTTTTACTCTTAATTTAA
Protein sequence

>MsG0580028722.01.T01

MGPFPWEPEGINNRCSIFSQTLAVNDATVKFEIWDTAGQERYHSLAPMYYRGAAAAIIVYDITNLESFTRAKKWVQELQKQGNPNMVVAFAGNKADLEDKRKVTAELHILYIPIRQLHLLELPTYPAHDSCTMQEARVYAEENGLFFIETSAKTAANVNDIFYEIGASPFLLHTLQVLDKSFILFLLLI*