Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029144.01.T01 | XP_003616568.1 | 98.529 | 272 | 3 | 1 | 1 | 272 | 1 | 271 | 0 | 558 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029144.01.T01 | Q8S9H7 | 58.411 | 214 | 78 | 4 | 3 | 207 | 1 | 212 | 2.17E-80 | 247 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029144.01.T01 | G7KBX0 | 98.529 | 272 | 3 | 1 | 1 | 272 | 1 | 271 | 0.0 | 558 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380017667.01 | MsG0580029144.01 | 0.808648 | 2.793042e-50 | 1.605708e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029144.01.T01 | MTR_5g081860 | 98.529 | 272 | 3 | 1 | 1 | 272 | 1 | 271 | 0.0 | 558 |
| MsG0580029144.01.T01 | MTR_7g067080 | 62.150 | 214 | 72 | 2 | 3 | 209 | 11 | 222 | 3.17e-90 | 270 |
| MsG0580029144.01.T01 | MTR_5g069710 | 61.386 | 202 | 71 | 3 | 2 | 198 | 4 | 203 | 9.91e-85 | 256 |
| MsG0580029144.01.T01 | MTR_3g462790 | 61.611 | 211 | 68 | 4 | 16 | 215 | 27 | 235 | 4.81e-83 | 252 |
| MsG0580029144.01.T01 | MTR_1g111830 | 48.020 | 202 | 91 | 4 | 25 | 219 | 22 | 216 | 4.40e-56 | 181 |
| MsG0580029144.01.T01 | MTR_8g063600 | 48.634 | 183 | 92 | 1 | 20 | 202 | 3 | 183 | 9.80e-51 | 168 |
| MsG0580029144.01.T01 | MTR_4g111975 | 42.381 | 210 | 81 | 5 | 27 | 201 | 8 | 212 | 2.54e-47 | 160 |
| MsG0580029144.01.T01 | MTR_8g063870 | 46.111 | 180 | 79 | 3 | 25 | 202 | 6 | 169 | 4.19e-42 | 145 |
| MsG0580029144.01.T01 | MTR_6g092540 | 61.538 | 91 | 32 | 2 | 119 | 208 | 91 | 179 | 2.90e-29 | 112 |
| MsG0580029144.01.T01 | MTR_2g100930 | 64.634 | 82 | 27 | 1 | 120 | 201 | 104 | 183 | 6.03e-29 | 113 |
| MsG0580029144.01.T01 | MTR_4g100630 | 64.198 | 81 | 27 | 1 | 120 | 200 | 90 | 168 | 8.17e-28 | 109 |
| MsG0580029144.01.T01 | MTR_4g015130 | 65.385 | 78 | 25 | 1 | 134 | 211 | 2 | 77 | 9.09e-28 | 102 |
| MsG0580029144.01.T01 | MTR_8g101650 | 60.714 | 84 | 30 | 1 | 116 | 199 | 96 | 176 | 8.34e-27 | 105 |
| MsG0580029144.01.T01 | MTR_5g037080 | 57.778 | 90 | 36 | 1 | 112 | 201 | 89 | 176 | 8.69e-26 | 103 |
| MsG0580029144.01.T01 | MTR_4g107230 | 71.186 | 59 | 17 | 0 | 113 | 171 | 72 | 130 | 1.24e-24 | 100 |
| MsG0580029144.01.T01 | MTR_5g027570 | 49.524 | 105 | 49 | 1 | 92 | 192 | 55 | 159 | 1.08e-23 | 96.3 |
| MsG0580029144.01.T01 | MTR_5g088010 | 58.904 | 73 | 29 | 1 | 121 | 192 | 56 | 128 | 4.84e-23 | 95.1 |
| MsG0580029144.01.T01 | MTR_1g033600 | 39.695 | 131 | 65 | 3 | 25 | 142 | 10 | 139 | 5.62e-23 | 92.0 |
| MsG0580029144.01.T01 | MTR_3g104370 | 51.429 | 105 | 44 | 4 | 90 | 191 | 58 | 158 | 1.21e-22 | 93.6 |
| MsG0580029144.01.T01 | MTR_5g088060 | 58.025 | 81 | 32 | 2 | 113 | 192 | 261 | 340 | 1.14e-21 | 94.4 |
| MsG0580029144.01.T01 | MTR_5g488160 | 58.025 | 81 | 32 | 2 | 113 | 192 | 261 | 340 | 1.14e-21 | 94.4 |
| MsG0580029144.01.T01 | MTR_1g033620 | 70.968 | 62 | 16 | 1 | 141 | 202 | 21 | 80 | 2.49e-21 | 90.5 |
| MsG0580029144.01.T01 | MTR_1g022290 | 55.882 | 68 | 30 | 0 | 22 | 89 | 2 | 69 | 4.99e-21 | 85.5 |
| MsG0580029144.01.T01 | MTR_0036s0260 | 57.971 | 69 | 27 | 1 | 125 | 193 | 28 | 94 | 2.97e-20 | 86.7 |
| MsG0580029144.01.T01 | MTR_2g090305 | 57.971 | 69 | 27 | 1 | 125 | 193 | 28 | 94 | 9.22e-20 | 84.7 |
| MsG0580029144.01.T01 | MTR_3g111880 | 53.846 | 65 | 30 | 0 | 25 | 89 | 5 | 69 | 1.69e-19 | 81.3 |
| MsG0580029144.01.T01 | MTR_5g027550 | 44.737 | 114 | 51 | 2 | 121 | 233 | 73 | 175 | 5.01e-19 | 84.0 |
| MsG0580029144.01.T01 | MTR_6g004250 | 52.941 | 68 | 32 | 0 | 22 | 89 | 5 | 72 | 1.12e-18 | 78.6 |
| MsG0580029144.01.T01 | MTR_7g089210 | 52.941 | 68 | 32 | 0 | 30 | 97 | 15 | 82 | 5.99e-18 | 77.0 |
| MsG0580029144.01.T01 | MTR_5g075760 | 34.706 | 170 | 102 | 6 | 26 | 194 | 12 | 173 | 7.88e-18 | 80.1 |
| MsG0580029144.01.T01 | MTR_3g070110 | 40.764 | 157 | 79 | 7 | 48 | 196 | 21 | 171 | 1.08e-17 | 79.7 |
| MsG0580029144.01.T01 | MTR_3g116720 | 52.381 | 63 | 30 | 0 | 27 | 89 | 16 | 78 | 1.98e-17 | 75.5 |
| MsG0580029144.01.T01 | MTR_5g020170 | 45.333 | 75 | 41 | 0 | 15 | 89 | 7 | 81 | 5.59e-17 | 74.7 |
| MsG0580029144.01.T01 | MTR_3g013440 | 47.945 | 73 | 37 | 1 | 18 | 89 | 1 | 73 | 1.48e-16 | 73.6 |
| MsG0580029144.01.T01 | MTR_1g048660 | 53.030 | 66 | 29 | 1 | 126 | 191 | 13 | 76 | 1.79e-16 | 73.2 |
| MsG0580029144.01.T01 | MTR_5g075790 | 31.176 | 170 | 107 | 4 | 26 | 194 | 21 | 181 | 3.63e-16 | 75.9 |
| MsG0580029144.01.T01 | MTR_8g077360 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.79e-15 | 69.3 |
| MsG0580029144.01.T01 | MTR_8g077380 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.95e-15 | 69.3 |
| MsG0580029144.01.T01 | MTR_8g077390 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.95e-15 | 69.3 |
| MsG0580029144.01.T01 | MTR_8g077420 | 50.820 | 61 | 29 | 1 | 27 | 87 | 5 | 64 | 5.75e-14 | 65.9 |
| MsG0580029144.01.T01 | MTR_5g020540 | 50.820 | 61 | 29 | 1 | 27 | 87 | 4 | 63 | 2.70e-13 | 63.9 |
| MsG0580029144.01.T01 | MTR_1g083180 | 55.714 | 70 | 29 | 2 | 127 | 195 | 63 | 131 | 3.26e-13 | 67.0 |
| MsG0580029144.01.T01 | MTR_3g111920 | 41.270 | 63 | 37 | 0 | 27 | 89 | 3 | 65 | 1.11e-12 | 62.8 |
| MsG0580029144.01.T01 | MTR_6g043490 | 41.026 | 78 | 41 | 2 | 125 | 197 | 107 | 184 | 6.19e-12 | 64.3 |
| MsG0580029144.01.T01 | MTR_6g043480 | 53.191 | 47 | 22 | 0 | 125 | 171 | 107 | 153 | 7.17e-12 | 63.9 |
| MsG0580029144.01.T01 | MTR_8g072990 | 50.000 | 50 | 25 | 0 | 122 | 171 | 97 | 146 | 1.06e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029144.01.T01 | AT2G38090 | 59.716 | 211 | 69 | 2 | 3 | 199 | 5 | 213 | 5.79e-86 | 259 |
| MsG0580029144.01.T01 | AT3G11280 | 64.039 | 203 | 57 | 5 | 3 | 196 | 1 | 196 | 5.12e-81 | 245 |
| MsG0580029144.01.T01 | AT3G11280 | 64.039 | 203 | 57 | 5 | 3 | 196 | 1 | 196 | 5.12e-81 | 245 |
| MsG0580029144.01.T01 | AT5G05790 | 58.605 | 215 | 80 | 5 | 3 | 210 | 4 | 216 | 7.24e-79 | 240 |
| MsG0580029144.01.T01 | AT5G05790 | 58.605 | 215 | 80 | 5 | 3 | 210 | 4 | 216 | 7.24e-79 | 240 |
| MsG0580029144.01.T01 | AT5G58900 | 57.277 | 213 | 72 | 4 | 16 | 211 | 15 | 225 | 9.42e-77 | 235 |
| MsG0580029144.01.T01 | AT5G01200 | 54.067 | 209 | 73 | 6 | 6 | 196 | 12 | 215 | 8.68e-67 | 209 |
| MsG0580029144.01.T01 | AT5G08520 | 51.087 | 184 | 80 | 2 | 27 | 202 | 12 | 193 | 7.58e-54 | 177 |
| MsG0580029144.01.T01 | AT1G49010 | 45.050 | 202 | 81 | 4 | 21 | 194 | 2 | 201 | 1.28e-49 | 166 |
| MsG0580029144.01.T01 | AT5G04760 | 44.898 | 196 | 94 | 5 | 25 | 217 | 4 | 188 | 1.54e-46 | 155 |
| MsG0580029144.01.T01 | AT5G23650 | 45.745 | 188 | 79 | 4 | 20 | 192 | 6 | 185 | 3.95e-44 | 152 |
| MsG0580029144.01.T01 | AT3G10580 | 37.981 | 208 | 98 | 7 | 27 | 232 | 8 | 186 | 4.68e-35 | 127 |
| MsG0580029144.01.T01 | AT3G10580 | 37.981 | 208 | 98 | 7 | 27 | 232 | 8 | 186 | 9.08e-35 | 127 |
| MsG0580029144.01.T01 | AT4G09450 | 42.515 | 167 | 80 | 4 | 26 | 192 | 6 | 156 | 6.11e-32 | 117 |
| MsG0580029144.01.T01 | AT5G56840 | 63.415 | 82 | 29 | 1 | 120 | 201 | 86 | 166 | 1.24e-27 | 107 |
| MsG0580029144.01.T01 | AT5G47390 | 66.250 | 80 | 25 | 1 | 112 | 191 | 83 | 160 | 1.28e-27 | 109 |
| MsG0580029144.01.T01 | AT5G61620 | 54.639 | 97 | 42 | 1 | 96 | 192 | 79 | 173 | 8.63e-27 | 106 |
| MsG0580029144.01.T01 | AT1G19000 | 51.327 | 113 | 49 | 3 | 112 | 220 | 89 | 199 | 1.95e-25 | 102 |
| MsG0580029144.01.T01 | AT1G19000 | 51.327 | 113 | 49 | 3 | 112 | 220 | 89 | 199 | 1.95e-25 | 102 |
| MsG0580029144.01.T01 | AT1G70000 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 6.84e-25 | 100 |
| MsG0580029144.01.T01 | AT1G70000 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 6.84e-25 | 100 |
| MsG0580029144.01.T01 | AT3G16350 | 57.732 | 97 | 36 | 2 | 116 | 209 | 125 | 219 | 2.79e-24 | 100 |
| MsG0580029144.01.T01 | AT3G10590 | 42.222 | 180 | 79 | 11 | 27 | 192 | 7 | 175 | 6.07e-23 | 94.0 |
| MsG0580029144.01.T01 | AT1G74840 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 5.98e-22 | 92.0 |
| MsG0580029144.01.T01 | AT1G74840 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 8.38e-22 | 92.0 |
| MsG0580029144.01.T01 | AT1G75250 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 6.82e-18 | 77.4 |
| MsG0580029144.01.T01 | AT1G75250 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 8.48e-18 | 77.8 |
| MsG0580029144.01.T01 | AT4G39250 | 48.485 | 66 | 34 | 0 | 27 | 92 | 14 | 79 | 1.56e-17 | 76.3 |
| MsG0580029144.01.T01 | AT2G21650 | 47.761 | 67 | 35 | 0 | 27 | 93 | 14 | 80 | 4.18e-16 | 72.4 |
| MsG0580029144.01.T01 | AT2G18328 | 54.098 | 61 | 27 | 1 | 29 | 89 | 14 | 73 | 4.45e-14 | 66.2 |
| MsG0580029144.01.T01 | AT1G19510 | 51.724 | 58 | 28 | 0 | 32 | 89 | 17 | 74 | 3.97e-13 | 64.7 |
| MsG0580029144.01.T01 | AT3G10595 | 29.054 | 148 | 81 | 5 | 24 | 171 | 3 | 126 | 3.34e-12 | 63.9 |
Find 73 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATTGCAGTTTGCTTTA+AGG | 0.090941 | 5:-92840567 | None:intergenic |
| CTCAATCCTTTAACAGAATT+TGG | 0.092721 | 5:+92840591 | MsG0580029144.01.T01:three_prime_UTR |
| TCCACTCCAAATTCTGTTAA+AGG | 0.150880 | 5:-92840597 | None:intergenic |
| GTAGTAGTATTTGTGATTTA+AGG | 0.198334 | 5:+92838947 | MsG0580029144.01.T01:five_prime_UTR |
| GTCTCCTATGCAGCAGAAAA+TGG | 0.222210 | 5:+92840345 | MsG0580029144.01.T01:CDS |
| TATGCTAAGCTGAAAGTTTC+GGG | 0.251091 | 5:+92840442 | MsG0580029144.01.T01:CDS |
| TCGGGTTTCGCGACAGCTTC+TGG | 0.263182 | 5:+92840460 | MsG0580029144.01.T01:CDS |
| ACCAGGAATCGGAACATTTC+CGG | 0.268577 | 5:-92839212 | None:intergenic |
| TGCAGTTTGCTTTAAGGTTC+AGG | 0.269442 | 5:-92840561 | None:intergenic |
| AGAAGATATGAAAATATTAT+AGG | 0.300464 | 5:-92838590 | None:intergenic |
| GCCGGAAATGTTCCGATTCC+TGG | 0.317971 | 5:+92839211 | MsG0580029144.01.T01:CDS |
| TTATGCTAAGCTGAAAGTTT+CGG | 0.326332 | 5:+92840441 | MsG0580029144.01.T01:CDS |
| CTTCTCTACTCCATTCTGTT+TGG | 0.332735 | 5:-92839039 | None:intergenic |
| TAGTGATCATGTCTATCTTT+TGG | 0.334038 | 5:+92840665 | MsG0580029144.01.T01:three_prime_UTR |
| AGGAGACACATTGACTAATA+AGG | 0.335368 | 5:-92840329 | None:intergenic |
| TGATGTGATTAAGCAGTATA+GGG | 0.335951 | 5:+92839159 | MsG0580029144.01.T01:CDS |
| ACATAAAATAAGGAGAATAA+AGG | 0.337256 | 5:-92838985 | None:intergenic |
| TGTGATTTAAGGTATGGATT+TGG | 0.345223 | 5:+92838958 | MsG0580029144.01.T01:exon |
| AGGGTGGTGTATAATATTCT+TGG | 0.349156 | 5:+92838670 | MsG0580029144.01.T01:five_prime_UTR |
| AGATTCTGTATGATACTAAA+AGG | 0.365142 | 5:+92838778 | MsG0580029144.01.T01:five_prime_UTR |
| AGAAGGCATGTGACTGTTAG+AGG | 0.384719 | 5:+92839295 | MsG0580029144.01.T01:CDS |
| TTGATGTGATTAAGCAGTAT+AGG | 0.386148 | 5:+92839158 | MsG0580029144.01.T01:CDS |
| AAATACTACTACAAAAGGTT+TGG | 0.386778 | 5:-92838936 | None:intergenic |
| AAGATTGCTGAGATGATTCC+TGG | 0.391655 | 5:+92839124 | MsG0580029144.01.T01:CDS |
| ATATTCAACCTTTCTTTAAG+AGG | 0.395823 | 5:+92838650 | MsG0580029144.01.T01:five_prime_UTR |
| AGTATTTGTGATTTAAGGTA+TGG | 0.403756 | 5:+92838952 | MsG0580029144.01.T01:exon |
| TTTGTATCCAACATAAAATA+AGG | 0.405866 | 5:-92838995 | None:intergenic |
| ATCACAAATACTACTACAAA+AGG | 0.412501 | 5:-92838941 | None:intergenic |
| ATAAGGCAAAAGGTTTCTTC+TGG | 0.415949 | 5:+92840220 | MsG0580029144.01.T01:CDS |
| GCAATCTTAAGCCATCTATC+TGG | 0.417904 | 5:-92839109 | None:intergenic |
| TAAAGTATGGCAAAGGCGAC+TGG | 0.445487 | 5:+92840125 | MsG0580029144.01.T01:CDS |
| CATAAAATAAGGAGAATAAA+GGG | 0.445828 | 5:-92838984 | None:intergenic |
| GATTAAGCAGTATAGGGAAT+TGG | 0.449715 | 5:+92839165 | MsG0580029144.01.T01:CDS |
| GCATGACTTGCTACTTGTGT+TGG | 0.455619 | 5:-92840184 | None:intergenic |
| TGCTCTTGCTATATATGATA+AGG | 0.457525 | 5:+92839081 | MsG0580029144.01.T01:CDS |
| TGAGACACAGCATGTCTGAA+TGG | 0.469648 | 5:-92840714 | None:intergenic |
| TACACCACCCTCTTAAAGAA+AGG | 0.470360 | 5:-92838658 | None:intergenic |
| ATAAGGATACACCAGATAGA+TGG | 0.475384 | 5:+92839098 | MsG0580029144.01.T01:CDS |
| ATGCACAAAAGTACTACATA+AGG | 0.480834 | 5:+92840203 | MsG0580029144.01.T01:CDS |
| AAGGACTTGTTGTTATCTGA+TGG | 0.489147 | 5:-92840310 | None:intergenic |
| AGAGGAAGAAAGGTGTTCCT+TGG | 0.489408 | 5:+92839329 | MsG0580029144.01.T01:CDS |
| CAAGAGCAAGAGGGTGAAAG+AGG | 0.493861 | 5:-92838561 | None:intergenic |
| GAGACACAGCATGTCTGAAT+GGG | 0.499137 | 5:-92840713 | None:intergenic |
| AAAGTACTACATAAGGCAAA+AGG | 0.510073 | 5:+92840210 | MsG0580029144.01.T01:CDS |
| TATTCAACCTTTCTTTAAGA+GGG | 0.510102 | 5:+92838651 | MsG0580029144.01.T01:five_prime_UTR |
| GTTGATGACATGAAGCTACT+TGG | 0.515565 | 5:+92840527 | MsG0580029144.01.T01:three_prime_UTR |
| GAAGCAAGATAACCAGGAAT+CGG | 0.518051 | 5:-92839223 | None:intergenic |
| TGATGGATCACCACAAGGGC+AGG | 0.524353 | 5:+92840390 | MsG0580029144.01.T01:CDS |
| GCTTCTGGAGACTTCAACAA+AGG | 0.531264 | 5:+92840475 | MsG0580029144.01.T01:CDS |
| TCTCCTATGCAGCAGAAAAT+GGG | 0.535185 | 5:+92840346 | MsG0580029144.01.T01:CDS |
| AGTACTAGCCACTACAATGA+TGG | 0.540673 | 5:+92840373 | MsG0580029144.01.T01:CDS |
| TCTGATCATGAGAGGAAGAA+AGG | 0.544690 | 5:+92839319 | MsG0580029144.01.T01:CDS |
| TCAACCTTTCTTTAAGAGGG+TGG | 0.551667 | 5:+92838654 | MsG0580029144.01.T01:five_prime_UTR |
| AAGAGAGTTACCAAACAGAA+TGG | 0.556624 | 5:+92839029 | MsG0580029144.01.T01:CDS |
| TCCTTTAACAGAATTTGGAG+TGG | 0.565291 | 5:+92840596 | MsG0580029144.01.T01:three_prime_UTR |
| AGGCAAAAGGTTTCTTCTGG+AGG | 0.582472 | 5:+92840223 | MsG0580029144.01.T01:CDS |
| AAGAGCAAGAGGGTGAAAGA+GGG | 0.582574 | 5:-92838560 | None:intergenic |
| ACTATGATGGAAACAGAAGA+AGG | 0.582942 | 5:+92839278 | MsG0580029144.01.T01:CDS |
| TCATAGAGATCCTGCCCTTG+TGG | 0.585420 | 5:-92840400 | None:intergenic |
| CTTGGACTGAAGAGGAACAT+AGG | 0.585629 | 5:+92839347 | MsG0580029144.01.T01:CDS |
| CTATGTTCCTCTTCAGTCCA+AGG | 0.586053 | 5:-92839346 | None:intergenic |
| CTATGCAGCAGAAAATGGGT+TGG | 0.587490 | 5:+92840350 | MsG0580029144.01.T01:CDS |
| AGGTGTTCCTTGGACTGAAG+AGG | 0.592742 | 5:+92839339 | MsG0580029144.01.T01:CDS |
| TAGGAACATTCAAGAGCAAG+AGG | 0.598552 | 5:-92838571 | None:intergenic |
| GTTGTGATATCATGAATGCT+TGG | 0.600782 | 5:-92840262 | None:intergenic |
| AAAGAAGAAGCAAGATAACC+AGG | 0.607746 | 5:-92839229 | None:intergenic |
| GATGTGAGTGAAATTGAGGC+CGG | 0.613302 | 5:+92839193 | MsG0580029144.01.T01:CDS |
| TACAATGATGGATCACCACA+AGG | 0.622159 | 5:+92840385 | MsG0580029144.01.T01:CDS |
| TTAGAGGTTCTGATCATGAG+AGG | 0.633143 | 5:+92839311 | MsG0580029144.01.T01:CDS |
| GTGGTGATCCATCATTGTAG+TGG | 0.633599 | 5:-92840381 | None:intergenic |
| AGAAGATGTGAGTGAAATTG+AGG | 0.635385 | 5:+92839189 | MsG0580029144.01.T01:CDS |
| AGGAACATTCAAGAGCAAGA+GGG | 0.687281 | 5:-92838570 | None:intergenic |
| ACAATGATGGATCACCACAA+GGG | 0.720384 | 5:+92840386 | MsG0580029144.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAAAACCTAAAATCT+AGG | - | Chr5:92839741-92839760 | None:intergenic | 15.0% |
| !! | AGAAGATATGAAAATATTAT+AGG | - | Chr5:92838593-92838612 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+AGG | - | Chr5:92839916-92839935 | None:intergenic | 15.0% |
| !!! | ATAACAAGTAACTTTTAAAA+GGG | - | Chr5:92839915-92839934 | None:intergenic | 15.0% |
| !! | ACATAAAATAAGGAGAATAA+AGG | - | Chr5:92838988-92839007 | None:intergenic | 20.0% |
| !! | AGTAAAAGAACAAAAAGAAA+AGG | - | Chr5:92839404-92839423 | None:intergenic | 20.0% |
| !! | ATTGAACTTGATTATTAGTT+TGG | + | Chr5:92839933-92839952 | MsG0580029144.01.T01:intron | 20.0% |
| !! | CATAAAATAAGGAGAATAAA+GGG | - | Chr5:92838987-92839006 | None:intergenic | 20.0% |
| !! | TTTGTATCCAACATAAAATA+AGG | - | Chr5:92838998-92839017 | None:intergenic | 20.0% |
| !!! | ATCTATCTTTTTTTCTCTAT+TGG | + | Chr5:92838520-92838539 | MsG0580029144.01.T01:five_prime_UTR | 20.0% |
| !!! | ATTTCATTTTCCTAGATTTT+AGG | + | Chr5:92839728-92839747 | MsG0580029144.01.T01:intron | 20.0% |
| !!! | ATTTTATGTTGGATACAAAT+TGG | + | Chr5:92838999-92839018 | MsG0580029144.01.T01:CDS | 20.0% |
| !!! | TAACAAGTAACTTTTAAAAG+GGG | - | Chr5:92839914-92839933 | None:intergenic | 20.0% |
| !!! | TTATTCTCCTTATTTTATGT+TGG | + | Chr5:92838988-92839007 | MsG0580029144.01.T01:CDS | 20.0% |
| !!! | TTCATATCTTCTATCTTTTT+TGG | + | Chr5:92838601-92838620 | MsG0580029144.01.T01:five_prime_UTR | 20.0% |
| !!! | TTTTTCTAGTAGTATACTAT+AGG | - | Chr5:92839471-92839490 | None:intergenic | 20.0% |
| ! | AAATACTACTACAAAAGGTT+TGG | - | Chr5:92838939-92838958 | None:intergenic | 25.0% |
| ! | AGAAAAATTGAAGTAAGACA+TGG | + | Chr5:92839484-92839503 | MsG0580029144.01.T01:intron | 25.0% |
| ! | AGAGACGGAAAAAATATAAT+CGG | + | Chr5:92839512-92839531 | MsG0580029144.01.T01:intron | 25.0% |
| ! | AGATTCTGTATGATACTAAA+AGG | + | Chr5:92838778-92838797 | MsG0580029144.01.T01:five_prime_UTR | 25.0% |
| ! | ATATTCAACCTTTCTTTAAG+AGG | + | Chr5:92838650-92838669 | MsG0580029144.01.T01:five_prime_UTR | 25.0% |
| ! | ATCACAAATACTACTACAAA+AGG | - | Chr5:92838944-92838963 | None:intergenic | 25.0% |
| ! | CATAAAAAACCACAAAAAAG+AGG | - | Chr5:92838905-92838924 | None:intergenic | 25.0% |
| ! | GTAGTAGTATTTGTGATTTA+AGG | + | Chr5:92838947-92838966 | MsG0580029144.01.T01:five_prime_UTR | 25.0% |
| ! | TAGCTAATGAATCATATTCA+TGG | + | Chr5:92839677-92839696 | MsG0580029144.01.T01:intron | 25.0% |
| !! | AGTATTTGTGATTTAAGGTA+TGG | + | Chr5:92838952-92838971 | MsG0580029144.01.T01:exon | 25.0% |
| !! | TATTCAACCTTTCTTTAAGA+GGG | + | Chr5:92838651-92838670 | MsG0580029144.01.T01:five_prime_UTR | 25.0% |
| !!! | ATGTTAAATTGAGTTGGTTT+AGG | + | Chr5:92840005-92840024 | MsG0580029144.01.T01:intron | 25.0% |
| !!! | CTATAATTTCCTCTTTTTTG+TGG | + | Chr5:92838893-92838912 | MsG0580029144.01.T01:five_prime_UTR | 25.0% |
| !!! | TTTGAGACTATTGATTTTGA+TGG | - | Chr5:92838702-92838721 | None:intergenic | 25.0% |
| ATCGACATGTTAAATTGAGT+TGG | + | Chr5:92839999-92840018 | MsG0580029144.01.T01:intron | 30.0% | |
| ATTTACTCTTTGTGCATGTT+TGG | + | Chr5:92840067-92840086 | MsG0580029144.01.T01:intron | 30.0% | |
| CTCAATCCTTTAACAGAATT+TGG | + | Chr5:92840591-92840610 | MsG0580029144.01.T01:three_prime_UTR | 30.0% | |
| GAAAAAACTTCCATAGAGAA+AGG | - | Chr5:92839427-92839446 | None:intergenic | 30.0% | |
| GTCGAAAAACAAAACTATGA+TGG | + | Chr5:92839265-92839284 | MsG0580029144.01.T01:CDS | 30.0% | |
| TGATGTGATTAAGCAGTATA+GGG | + | Chr5:92839159-92839178 | MsG0580029144.01.T01:CDS | 30.0% | |
| TTATGCTAAGCTGAAAGTTT+CGG | + | Chr5:92840441-92840460 | MsG0580029144.01.T01:CDS | 30.0% | |
| TTGATGTGATTAAGCAGTAT+AGG | + | Chr5:92839158-92839177 | MsG0580029144.01.T01:CDS | 30.0% | |
| ! | AAAGTACTACATAAGGCAAA+AGG | + | Chr5:92840210-92840229 | MsG0580029144.01.T01:CDS | 30.0% |
| ! | CATTATTGCAGTTTGCTTTA+AGG | - | Chr5:92840570-92840589 | None:intergenic | 30.0% |
| ! | TAGTGATCATGTCTATCTTT+TGG | + | Chr5:92840665-92840684 | MsG0580029144.01.T01:three_prime_UTR | 30.0% |
| ! | TGCTCTTGCTATATATGATA+AGG | + | Chr5:92839081-92839100 | MsG0580029144.01.T01:CDS | 30.0% |
| ! | TGTGATTTAAGGTATGGATT+TGG | + | Chr5:92838958-92838977 | MsG0580029144.01.T01:exon | 30.0% |
| ! | TTCTTTTACTCCTTTCTCTA+TGG | + | Chr5:92839414-92839433 | MsG0580029144.01.T01:intron | 30.0% |
| !! | ATGCACAAAAGTACTACATA+AGG | + | Chr5:92840203-92840222 | MsG0580029144.01.T01:CDS | 30.0% |
| !! | GTAACTTTTAAAAGGGGTAA+AGG | - | Chr5:92839908-92839927 | None:intergenic | 30.0% |
| !! | TTGTTTTTCGACAACTTCAA+AGG | - | Chr5:92839257-92839276 | None:intergenic | 30.0% |
| !!! | GGAAGTTTTTTCTTAGATTG+TGG | + | Chr5:92839435-92839454 | MsG0580029144.01.T01:intron | 30.0% |
| AAAGAAGAAGCAAGATAACC+AGG | - | Chr5:92839232-92839251 | None:intergenic | 35.0% | |
| AAGAGAGTTACCAAACAGAA+TGG | + | Chr5:92839029-92839048 | MsG0580029144.01.T01:CDS | 35.0% | |
| ACATCAAAAACAGTCTTACC+AGG | - | Chr5:92839145-92839164 | None:intergenic | 35.0% | |
| AGAAGATGTGAGTGAAATTG+AGG | + | Chr5:92839189-92839208 | MsG0580029144.01.T01:CDS | 35.0% | |
| AGGGTGGTGTATAATATTCT+TGG | + | Chr5:92838670-92838689 | MsG0580029144.01.T01:five_prime_UTR | 35.0% | |
| ATAAGGATACACCAGATAGA+TGG | + | Chr5:92839098-92839117 | MsG0580029144.01.T01:CDS | 35.0% | |
| ATAAGGCAAAAGGTTTCTTC+TGG | + | Chr5:92840220-92840239 | MsG0580029144.01.T01:CDS | 35.0% | |
| GAGTCTGTAACACTTAATGA+TGG | + | Chr5:92839546-92839565 | MsG0580029144.01.T01:intron | 35.0% | |
| GATTAAGCAGTATAGGGAAT+TGG | + | Chr5:92839165-92839184 | MsG0580029144.01.T01:CDS | 35.0% | |
| GTTGTGATATCATGAATGCT+TGG | - | Chr5:92840265-92840284 | None:intergenic | 35.0% | |
| TATGCTAAGCTGAAAGTTTC+GGG | + | Chr5:92840442-92840461 | MsG0580029144.01.T01:CDS | 35.0% | |
| TCCACTCCAAATTCTGTTAA+AGG | - | Chr5:92840600-92840619 | None:intergenic | 35.0% | |
| TCCTTTAACAGAATTTGGAG+TGG | + | Chr5:92840596-92840615 | MsG0580029144.01.T01:three_prime_UTR | 35.0% | |
| ! | ACTATGATGGAAACAGAAGA+AGG | + | Chr5:92839278-92839297 | MsG0580029144.01.T01:CDS | 35.0% |
| ! | AGGAGACACATTGACTAATA+AGG | - | Chr5:92840332-92840351 | None:intergenic | 35.0% |
| ! | TTTGTGCATGTTTGGAATCA+CGG | + | Chr5:92840075-92840094 | MsG0580029144.01.T01:intron | 35.0% |
| !! | AAGGACTTGTTGTTATCTGA+TGG | - | Chr5:92840313-92840332 | None:intergenic | 35.0% |
| !! | GTAACTTTTAAAAGGGGCAA+AGG | - | Chr5:92840037-92840056 | None:intergenic | 35.0% |
| !! | TTCAACAAAGGAGCTGTTTT+TGG | + | Chr5:92840487-92840506 | MsG0580029144.01.T01:CDS | 35.0% |
| AAGATTGCTGAGATGATTCC+TGG | + | Chr5:92839124-92839143 | MsG0580029144.01.T01:CDS | 40.0% | |
| AGGAACATTCAAGAGCAAGA+GGG | - | Chr5:92838573-92838592 | None:intergenic | 40.0% | |
| AGTACTAGCCACTACAATGA+TGG | + | Chr5:92840373-92840392 | MsG0580029144.01.T01:CDS | 40.0% | |
| CTTCTCTACTCCATTCTGTT+TGG | - | Chr5:92839042-92839061 | None:intergenic | 40.0% | |
| GAAGCAAGATAACCAGGAAT+CGG | - | Chr5:92839226-92839245 | None:intergenic | 40.0% | |
| GCAATCTTAAGCCATCTATC+TGG | - | Chr5:92839112-92839131 | None:intergenic | 40.0% | |
| GTAAAGGCAAACTCCAATTG+AGG | - | Chr5:92839892-92839911 | None:intergenic | 40.0% | |
| TAAGACATGGAAGCTAGAGA+CGG | + | Chr5:92839497-92839516 | MsG0580029144.01.T01:intron | 40.0% | |
| TACACCACCCTCTTAAAGAA+AGG | - | Chr5:92838661-92838680 | None:intergenic | 40.0% | |
| TAGGAACATTCAAGAGCAAG+AGG | - | Chr5:92838574-92838593 | None:intergenic | 40.0% | |
| TCTCCTATGCAGCAGAAAAT+GGG | + | Chr5:92840346-92840365 | MsG0580029144.01.T01:CDS | 40.0% | |
| TCTGATCATGAGAGGAAGAA+AGG | + | Chr5:92839319-92839338 | MsG0580029144.01.T01:CDS | 40.0% | |
| ! | CGTATAGACGTTTTCTGATG+GGG | + | Chr5:92840098-92840117 | MsG0580029144.01.T01:intron | 40.0% |
| ! | CTATGACTGCTCTTTTCATG+AGG | + | Chr5:92840417-92840436 | MsG0580029144.01.T01:CDS | 40.0% |
| ! | GCGTATAGACGTTTTCTGAT+GGG | + | Chr5:92840097-92840116 | MsG0580029144.01.T01:intron | 40.0% |
| ! | TCAACCTTTCTTTAAGAGGG+TGG | + | Chr5:92838654-92838673 | MsG0580029144.01.T01:five_prime_UTR | 40.0% |
| ! | TTAGAGGTTCTGATCATGAG+AGG | + | Chr5:92839311-92839330 | MsG0580029144.01.T01:CDS | 40.0% |
| !! | ACAATGATGGATCACCACAA+GGG | + | Chr5:92840386-92840405 | MsG0580029144.01.T01:CDS | 40.0% |
| !! | GTTGATGACATGAAGCTACT+TGG | + | Chr5:92840527-92840546 | MsG0580029144.01.T01:three_prime_UTR | 40.0% |
| !! | TACAATGATGGATCACCACA+AGG | + | Chr5:92840385-92840404 | MsG0580029144.01.T01:CDS | 40.0% |
| !! | TGCAGTTTGCTTTAAGGTTC+AGG | - | Chr5:92840564-92840583 | None:intergenic | 40.0% |
| !!! | CTGATGGGGCTTTTAAAGTA+TGG | + | Chr5:92840112-92840131 | MsG0580029144.01.T01:CDS | 40.0% |
| !!! | GGGCTTTTAAAGTATGGCAA+AGG | + | Chr5:92840118-92840137 | MsG0580029144.01.T01:CDS | 40.0% |
| AAGAGCAAGAGGGTGAAAGA+GGG | - | Chr5:92838563-92838582 | None:intergenic | 45.0% | |
| ACCAGGAATCGGAACATTTC+CGG | - | Chr5:92839215-92839234 | None:intergenic | 45.0% | |
| AGGCAAAAGGTTTCTTCTGG+AGG | + | Chr5:92840223-92840242 | MsG0580029144.01.T01:CDS | 45.0% | |
| CTATGCAGCAGAAAATGGGT+TGG | + | Chr5:92840350-92840369 | MsG0580029144.01.T01:CDS | 45.0% | |
| CTATGTTCCTCTTCAGTCCA+AGG | - | Chr5:92839349-92839368 | None:intergenic | 45.0% | |
| CTTGGACTGAAGAGGAACAT+AGG | + | Chr5:92839347-92839366 | MsG0580029144.01.T01:CDS | 45.0% | |
| GAGACACAGCATGTCTGAAT+GGG | - | Chr5:92840716-92840735 | None:intergenic | 45.0% | |
| GATGTGAGTGAAATTGAGGC+CGG | + | Chr5:92839193-92839212 | MsG0580029144.01.T01:CDS | 45.0% | |
| GCAGATAATGCAGCCTCAAT+TGG | + | Chr5:92839876-92839895 | MsG0580029144.01.T01:intron | 45.0% | |
| GTCTCCTATGCAGCAGAAAA+TGG | + | Chr5:92840345-92840364 | MsG0580029144.01.T01:CDS | 45.0% | |
| GTGGTGATCCATCATTGTAG+TGG | - | Chr5:92840384-92840403 | None:intergenic | 45.0% | |
| TAAAGTATGGCAAAGGCGAC+TGG | + | Chr5:92840125-92840144 | MsG0580029144.01.T01:CDS | 45.0% | |
| TGAGACACAGCATGTCTGAA+TGG | - | Chr5:92840717-92840736 | None:intergenic | 45.0% | |
| ! | AGAAGGCATGTGACTGTTAG+AGG | + | Chr5:92839295-92839314 | MsG0580029144.01.T01:CDS | 45.0% |
| ! | CAACCCATTTTCTGCTGCAT+AGG | - | Chr5:92840352-92840371 | None:intergenic | 45.0% |
| ! | GCTTCTGGAGACTTCAACAA+AGG | + | Chr5:92840475-92840494 | MsG0580029144.01.T01:CDS | 45.0% |
| ! | GGCGTATAGACGTTTTCTGA+TGG | + | Chr5:92840096-92840115 | MsG0580029144.01.T01:intron | 45.0% |
| !! | AGAGGAAGAAAGGTGTTCCT+TGG | + | Chr5:92839329-92839348 | MsG0580029144.01.T01:CDS | 45.0% |
| !! | GCATGACTTGCTACTTGTGT+TGG | - | Chr5:92840187-92840206 | None:intergenic | 45.0% |
| AGGTGTTCCTTGGACTGAAG+AGG | + | Chr5:92839339-92839358 | MsG0580029144.01.T01:CDS | 50.0% | |
| CAAGAGCAAGAGGGTGAAAG+AGG | - | Chr5:92838564-92838583 | None:intergenic | 50.0% | |
| TCATAGAGATCCTGCCCTTG+TGG | - | Chr5:92840403-92840422 | None:intergenic | 50.0% | |
| GCCGGAAATGTTCCGATTCC+TGG | + | Chr5:92839211-92839230 | MsG0580029144.01.T01:CDS | 55.0% | |
| ! | TGATGGATCACCACAAGGGC+AGG | + | Chr5:92840390-92840409 | MsG0580029144.01.T01:CDS | 55.0% |
| TCGGGTTTCGCGACAGCTTC+TGG | + | Chr5:92840460-92840479 | MsG0580029144.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 92838514 | 92840793 | 92838514 | ID=MsG0580029144.01;Name=MsG0580029144.01 |
| Chr5 | mRNA | 92838514 | 92840793 | 92838514 | ID=MsG0580029144.01.T01;Parent=MsG0580029144.01;Name=MsG0580029144.01.T01;_AED=0.38;_eAED=0.38;_QI=457|1|1|1|1|1|2|268|272 |
| Chr5 | exon | 92838514 | 92839368 | 92838514 | ID=MsG0580029144.01.T01:exon:17911;Parent=MsG0580029144.01.T01 |
| Chr5 | exon | 92840105 | 92840793 | 92840105 | ID=MsG0580029144.01.T01:exon:17912;Parent=MsG0580029144.01.T01 |
| Chr5 | five_prime_UTR | 92838514 | 92838970 | 92838514 | ID=MsG0580029144.01.T01:five_prime_utr;Parent=MsG0580029144.01.T01 |
| Chr5 | CDS | 92838971 | 92839368 | 92838971 | ID=MsG0580029144.01.T01:cds;Parent=MsG0580029144.01.T01 |
| Chr5 | CDS | 92840105 | 92840525 | 92840105 | ID=MsG0580029144.01.T01:cds;Parent=MsG0580029144.01.T01 |
| Chr5 | three_prime_UTR | 92840526 | 92840793 | 92840526 | ID=MsG0580029144.01.T01:three_prime_utr;Parent=MsG0580029144.01.T01 |
| Gene Sequence |
| Protein sequence |
