AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029330.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029330.01.T01 MTR_5g082660 95.298 319 1 3 8 326 1 305 0.0 603
MsG0580029330.01.T01 MTR_3g070810 64.918 305 74 6 20 323 4 276 1.08e-129 371
MsG0580029330.01.T01 MTR_3g006690 43.066 137 74 4 174 309 332 465 5.58e-21 93.6
MsG0580029330.01.T01 MTR_2g049420 40.441 136 77 4 174 308 196 328 1.02e-20 91.3
MsG0580029330.01.T01 MTR_2g049420 40.441 136 77 4 174 308 333 465 2.72e-20 91.7
MsG0580029330.01.T01 MTR_2g049420 40.441 136 77 4 174 308 324 456 3.16e-20 91.3
MsG0580029330.01.T01 MTR_5g045920 37.857 140 83 4 174 312 162 298 1.61e-19 87.8
MsG0580029330.01.T01 MTR_2g017810 42.593 108 59 2 166 273 139 243 3.52e-16 78.2
MsG0580029330.01.T01 MTR_3g085395 36.296 135 82 4 179 312 241 372 8.08e-14 72.0
MsG0580029330.01.T01 MTR_3g085400 35.714 140 86 4 174 312 358 494 2.75e-13 70.9
MsG0580029330.01.T01 MTR_5g095530 28.244 131 90 3 176 304 215 343 3.39e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029330.01.T01 AT3G55960 62.848 323 94 5 8 324 1 303 3.33e-140 399
MsG0580029330.01.T01 AT1G29780 37.073 205 94 9 92 287 21 199 1.42e-22 94.4
MsG0580029330.01.T01 AT1G29770 38.125 160 74 5 115 273 105 240 5.57e-20 88.6
MsG0580029330.01.T01 AT5G11860 37.324 142 85 4 172 312 146 284 1.93e-19 87.4
MsG0580029330.01.T01 AT5G11860 37.324 142 85 4 172 312 146 284 1.93e-19 87.4
MsG0580029330.01.T01 AT5G11860 37.324 142 85 4 172 312 146 284 1.93e-19 87.4
MsG0580029330.01.T01 AT5G11860 37.324 142 85 4 172 312 146 284 1.93e-19 87.4
MsG0580029330.01.T01 AT5G11860 37.324 142 85 4 172 312 146 284 1.93e-19 87.4
MsG0580029330.01.T01 AT4G18140 40.714 140 79 4 172 310 303 439 6.89e-19 87.4
MsG0580029330.01.T01 AT4G18140 41.304 138 77 4 172 308 303 437 1.04e-18 86.7
MsG0580029330.01.T01 AT4G18140 41.304 138 77 4 172 308 303 437 1.04e-18 86.7
MsG0580029330.01.T01 AT4G18140 41.304 138 77 4 172 308 303 437 1.04e-18 86.7
MsG0580029330.01.T01 AT4G18140 41.304 138 77 4 172 308 303 437 1.10e-18 86.7
MsG0580029330.01.T01 AT4G18140 41.304 138 77 4 172 308 303 437 1.10e-18 86.7
MsG0580029330.01.T01 AT5G45700 47.000 100 51 2 176 274 137 235 1.81e-18 84.3
MsG0580029330.01.T01 AT5G46410 40.580 138 78 4 172 308 318 452 4.45e-18 85.1
MsG0580029330.01.T01 AT5G46410 40.580 138 78 4 172 308 315 449 5.31e-18 84.7
MsG0580029330.01.T01 AT5G46410 40.580 138 78 4 172 308 315 449 5.31e-18 84.7
MsG0580029330.01.T01 AT1G55900 31.333 150 93 6 176 318 214 360 1.52e-15 77.0
MsG0580029330.01.T01 AT1G55900 32.061 131 84 4 176 304 214 341 2.45e-15 76.6

Find 79 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCCTTCCGTTGAACTTC+AGG 0.280216 5:-95365029 MsG0580029330.01.T01:CDS
GGAATCGATTATTGTTGTTA+TGG 0.291494 5:-95365248 MsG0580029330.01.T01:CDS
ATACATGGAATTCCATTTAC+TGG 0.300795 5:+95361497 None:intergenic
CAGATGCTCAAGAAATAAAT+TGG 0.307573 5:+95361091 None:intergenic
TGGCAATCTACATACATAAA+TGG 0.316489 5:+95361029 None:intergenic
TGGTTTGAATTGGTTTGATA+TGG 0.319744 5:-95363436 MsG0580029330.01.T01:CDS
AAGCTGGAATCCCTTGCTTC+TGG 0.326322 5:+95361240 None:intergenic
CAATCGAAGCTGGTTTGAAT+TGG 0.345306 5:-95363446 MsG0580029330.01.T01:CDS
TGTGGATGCCCAGCAGAAAA+TGG 0.348481 5:+95361473 None:intergenic
TTTGTATTGCAACCAGTAAA+TGG 0.348553 5:-95361509 MsG0580029330.01.T01:CDS
ATAGACAAGAATTAAAATTC+TGG 0.353569 5:+95361009 None:intergenic
GAATCGATTATTGTTGTTAT+GGG 0.359959 5:-95365247 MsG0580029330.01.T01:CDS
GAAATTCTTAGTTATATTGC+TGG 0.368052 5:-95361157 MsG0580029330.01.T01:three_prime_UTR
GATTCAGTTTACGACTTTAC+CGG 0.385425 5:-95363062 MsG0580029330.01.T01:CDS
TTGATCGGCGGCGGGAAAAT+CGG 0.396899 5:+95364970 None:intergenic
CTTTGAACGGCCTGGGTTGA+AGG 0.399020 5:-95363280 MsG0580029330.01.T01:CDS
CACCACGAACTCAACAAGTT+TGG 0.410118 5:-95365195 MsG0580029330.01.T01:CDS
GTTACAGTCTTTGAACGGCC+TGG 0.419543 5:-95363288 MsG0580029330.01.T01:CDS
GAAAATCGGCGGTGATTTCA+AGG 0.443582 5:+95364984 None:intergenic
TTACAGTCTTTGAACGGCCT+GGG 0.443967 5:-95363287 MsG0580029330.01.T01:CDS
TCTATAGGCTTGCAATCTGT+TGG 0.451533 5:-95360991 MsG0580029330.01.T01:three_prime_UTR
TACAATGTCCAACTGGAAGC+TGG 0.452345 5:+95361224 None:intergenic
GAAAGGGCGTTGATCGGCGG+CGG 0.453268 5:+95364961 None:intergenic
GTCAAGATCCTTCGATATGC+AGG 0.466355 5:+95361565 None:intergenic
AGCTTCCAGTTGGACATTGT+AGG 0.472847 5:-95361222 MsG0580029330.01.T01:exon
TCTCAGATTATTCGAACCCT+TGG 0.474766 5:-95365130 MsG0580029330.01.T01:CDS
ACCTACGTGCTAATTGTTGA+AGG 0.482376 5:+95363039 None:intergenic
GTGTTCTCATTTAGGCGTGT+TGG 0.483399 5:-95363350 MsG0580029330.01.T01:intron
CTGTTTACTGCGGGTCTCGA+AGG 0.487308 5:-95363219 MsG0580029330.01.T01:intron
TAAATCCTACAATGTCCAAC+TGG 0.492585 5:+95361217 None:intergenic
CGTTCTCAAGCAATCGAAGC+TGG 0.492682 5:-95363456 MsG0580029330.01.T01:CDS
GGTGATTTCAAGGGCGGACG+AGG 0.493305 5:+95364994 None:intergenic
CTTGTAGGTAGTACTTGACT+TGG 0.496374 5:-95363529 MsG0580029330.01.T01:intron
GATGATGAAGAAGAGAGTAA+AGG 0.499279 5:+95365100 None:intergenic
TATATGCTTGCAGGGAACAT+AGG 0.502070 5:-95361607 MsG0580029330.01.T01:intron
TTCCAAACTTGTTGAGTTCG+TGG 0.502398 5:+95365193 None:intergenic
GCTTGAGAACGTAGTGCAGC+TGG 0.502523 5:+95363468 None:intergenic
CCAGCAGAAAATGGAATACA+TGG 0.507302 5:+95361482 None:intergenic
AAAGGGCGTTGATCGGCGGC+GGG 0.519336 5:+95364962 None:intergenic
ATATGCTTGCAGGGAACATA+GGG 0.531954 5:-95361606 MsG0580029330.01.T01:intron
TTTATTTCTTGAGCATCTGA+AGG 0.533215 5:-95361087 MsG0580029330.01.T01:three_prime_UTR
TGAAGTTCAACGGAAGGAAG+AGG 0.534102 5:+95365031 None:intergenic
GTTAAGAATTCCTTCAACCC+AGG 0.544659 5:+95363270 None:intergenic
GACCTGGTGCTGTTTACTGC+GGG 0.551658 5:-95363228 MsG0580029330.01.T01:CDS
CTGATTGGTTCCAGAAGCAA+GGG 0.555776 5:-95361250 MsG0580029330.01.T01:CDS
GATTTAAATTGTTTGCCGCA+AGG 0.556155 5:-95361200 MsG0580029330.01.T01:three_prime_UTR
AGAGTAAAGGATAGTGACCA+AGG 0.564333 5:+95365113 None:intergenic
ACTGATTGGTTCCAGAAGCA+AGG 0.564868 5:-95361251 MsG0580029330.01.T01:CDS
TATGGGCGAGTTGACTCAAA+CGG 0.567062 5:-95365230 MsG0580029330.01.T01:CDS
TTGGAAAGGGCGTTGATCGG+CGG 0.569223 5:+95364958 None:intergenic
CTGGAAAGTGCAGATGCTGA+TGG 0.573639 5:-95361138 MsG0580029330.01.T01:three_prime_UTR
TCAGAGAGTTGCTTGAGAAG+TGG 0.576195 5:+95361317 None:intergenic
ATTATGTTACAGTCTTTGAA+CGG 0.582885 5:-95363293 MsG0580029330.01.T01:CDS
ACTTAGTGAATTTGCTGACC+TGG 0.585034 5:-95363244 MsG0580029330.01.T01:CDS
ACGTGCTAATTGTTGAAGGC+CGG 0.585930 5:+95363043 None:intergenic
ATCGAAATCCTGAAGTTCAA+CGG 0.591634 5:+95365021 None:intergenic
AAAATCGGCGGTGATTTCAA+GGG 0.591714 5:+95364985 None:intergenic
TAGTGTACCTGGGTGTCATG+TGG 0.595285 5:+95361455 None:intergenic
GACCCGCAGTAAACAGCACC+AGG 0.597074 5:+95363226 None:intergenic
GGAACATAGGGAACATGTCA+AGG 0.601739 5:-95361594 MsG0580029330.01.T01:CDS
TGACCTGGTGCTGTTTACTG+CGG 0.602861 5:-95363229 MsG0580029330.01.T01:CDS
AAGGGATTCCAGCTTCCAGT+TGG 0.603406 5:-95361232 MsG0580029330.01.T01:CDS
AAATCCTGAAGTTCAACGGA+AGG 0.606458 5:+95365025 None:intergenic
GATTTCAAGGGCGGACGAGG+AGG 0.610160 5:+95364997 None:intergenic
GGGCGAGTTGACTCAAACGG+AGG 0.611472 5:-95365227 MsG0580029330.01.T01:CDS
GGTTGCAATACAAAGCTGAA+AGG 0.613648 5:+95361518 None:intergenic
GGATCTTACCTGCATATCGA+AGG 0.615597 5:-95361573 MsG0580029330.01.T01:CDS
CAAACTTGTTGAGTTCGTGG+TGG 0.618165 5:+95365196 None:intergenic
GAGTAAAGGATAGTGACCAA+GGG 0.620789 5:+95365114 None:intergenic
TCTGGAACCAATCAGTCATG+TGG 0.624098 5:+95361258 None:intergenic
ATCGGCGGCGGGAAAATCGG+CGG 0.631500 5:+95364973 None:intergenic
TGGAAAGTGCAGATGCTGAT+GGG 0.635572 5:-95361137 MsG0580029330.01.T01:three_prime_UTR
TTGTCTATTATGTCAACTAG+CGG 0.643882 5:+95363093 None:intergenic
TGGGCATCCACATGACACCC+AGG 0.657885 5:-95361462 MsG0580029330.01.T01:intron
ATCGGCGGTGATTTCAAGGG+CGG 0.661076 5:+95364988 None:intergenic
GGAATGTGTATCTTCTGACA+AGG 0.672929 5:-95363415 MsG0580029330.01.T01:intron
GCTAGTTGACATAATAGACA+AGG 0.714010 5:-95363091 MsG0580029330.01.T01:CDS
AATGAAACAAAAGAGCCTTG+CGG 0.771468 5:+95361185 None:intergenic
TGTTGTCTACAATTACAATG+CGG 0.772106 5:+95361543 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTTATAATAATTAACTA+AGG + Chr5:95363517-95363536 None:intergenic 10.0%
!! ATAATAAAAGTAAAAAACAT+TGG + Chr5:95362432-95362451 None:intergenic 10.0%
!! TAATAAAAGTAAAAAACATT+GGG + Chr5:95362431-95362450 None:intergenic 10.0%
!! ATTACACAAATATATAACAT+TGG + Chr5:95363181-95363200 None:intergenic 15.0%
!! TGTTATTATATAATACAACA+TGG - Chr5:95361529-95361548 MsG0580029330.01.T01:CDS 15.0%
!! TTTATAATAATTAACTAAGG+AGG + Chr5:95363514-95363533 None:intergenic 15.0%
!!! AAAATTTTTGAAATACAACA+AGG + Chr5:95361762-95361781 None:intergenic 15.0%
!!! ACTGTAACATAATTAATTTT+TGG + Chr5:95362874-95362893 None:intergenic 15.0%
!! AAAACATAAGAATACTAGAT+GGG + Chr5:95364786-95364805 None:intergenic 20.0%
!! ACAAATATATAACATTGGAA+TGG + Chr5:95363176-95363195 None:intergenic 20.0%
!! ATAACATTTATAAGAATGCA+TGG + Chr5:95364550-95364569 None:intergenic 20.0%
!! ATAATTGTAAAATTCGAGAA+AGG + Chr5:95364480-95364499 None:intergenic 20.0%
!! ATAGACAAGAATTAAAATTC+TGG + Chr5:95365168-95365187 None:intergenic 20.0%
!! TAAAACATAAGAATACTAGA+TGG + Chr5:95364787-95364806 None:intergenic 20.0%
!! TGTAAGCACTATAATTAATA+AGG + Chr5:95363772-95363791 None:intergenic 20.0%
!! TTTATATGTCAAAGTTTCTT+AGG - Chr5:95361437-95361456 MsG0580029330.01.T01:intron 20.0%
!!! GAATTTTAATTCTTGTCTAT+AGG - Chr5:95365168-95365187 MsG0580029330.01.T01:CDS 20.0%
!!! GGGTGATTTTATTTTTTTAT+GGG + Chr5:95364766-95364785 None:intergenic 20.0%
!!! TATTTAATACCGTAAGTTTT+TGG + Chr5:95361238-95361257 None:intergenic 20.0%
!!! TGGGTGATTTTATTTTTTTA+TGG + Chr5:95364767-95364786 None:intergenic 20.0%
!!! TGTCTGTATTTAAAATATCA+TGG - Chr5:95364375-95364394 MsG0580029330.01.T01:intron 20.0%
!!! TTTACGTACTTTTGATTTTA+TGG - Chr5:95363958-95363977 MsG0580029330.01.T01:intron 20.0%
!!! TTTTTTGTTCGTTGAAAATT+TGG - Chr5:95363869-95363888 MsG0580029330.01.T01:intron 20.0%
! AACTAAAAAAAACTCACATC+TGG - Chr5:95362341-95362360 MsG0580029330.01.T01:intron 25.0%
! AATACACAACAAAGCAAATA+AGG + Chr5:95361817-95361836 None:intergenic 25.0%
! ACTTATTAACTCGCATTTAA+GGG + Chr5:95361847-95361866 None:intergenic 25.0%
! AGAGCAACAGTTAAATATTA+AGG + Chr5:95363573-95363592 None:intergenic 25.0%
! ATAAATGTTATATGCTTGCA+GGG - Chr5:95364559-95364578 MsG0580029330.01.T01:intron 25.0%
! ATCATAGAAAATAGTCTTCT+GGG - Chr5:95362033-95362052 MsG0580029330.01.T01:intron 25.0%
! ATTATGTTACAGTCTTTGAA+CGG - Chr5:95362881-95362900 MsG0580029330.01.T01:intron 25.0%
! GAAATTCTTAGTTATATTGC+TGG - Chr5:95365017-95365036 MsG0580029330.01.T01:CDS 25.0%
! TAATATCTTCAAAGTCCTAA+GGG - Chr5:95363935-95363954 MsG0580029330.01.T01:intron 25.0%
! TACTATCAAATTAGAACTAC+TGG - Chr5:95364269-95364288 MsG0580029330.01.T01:intron 25.0%
! TATAAATGTTATATGCTTGC+AGG - Chr5:95364558-95364577 MsG0580029330.01.T01:intron 25.0%
! TGACTATAACTCAAGTAATT+GGG + Chr5:95364134-95364153 None:intergenic 25.0%
! TTAACTAAAGAGACTTATTG+TGG + Chr5:95363645-95363664 None:intergenic 25.0%
! TTTAAATACAGACAGACATA+TGG + Chr5:95364369-95364388 None:intergenic 25.0%
!! AAACAGTTGTGTTGATAAAA+AGG + Chr5:95363904-95363923 None:intergenic 25.0%
!! ACTGTTGCATATTTGTATTT+TGG - Chr5:95363000-95363019 MsG0580029330.01.T01:intron 25.0%
!! AGAATTTTGTGTTCTCATTT+AGG - Chr5:95362816-95362835 MsG0580029330.01.T01:intron 25.0%
!! ATTTTCAACGAACAAAAAAG+AGG + Chr5:95363868-95363887 None:intergenic 25.0%
!! GAATCGATTATTGTTGTTAT+GGG - Chr5:95360927-95360946 MsG0580029330.01.T01:three_prime_UTR 25.0%
!! GCTAAAAATCCATAATGTTT+TGG - Chr5:95362141-95362160 MsG0580029330.01.T01:intron 25.0%
!! TCAATCATCTTAAAGGTTTT+TGG - Chr5:95361955-95361974 MsG0580029330.01.T01:intron 25.0%
!! TGGGTGATTATAATTTTCAA+TGG + Chr5:95364747-95364766 None:intergenic 25.0%
!! TTTCTTAGGTGAATTGTTTT+GGG - Chr5:95361451-95361470 MsG0580029330.01.T01:intron 25.0%
!! TTTTTATCAACACAACTGTT+TGG - Chr5:95363903-95363922 MsG0580029330.01.T01:intron 25.0%
!! TTTTTGTGTCATATTCTTAC+AGG - Chr5:95363048-95363067 MsG0580029330.01.T01:CDS 25.0%
!!! TAAAATATTTTGACCCTTGT+TGG - Chr5:95363981-95364000 MsG0580029330.01.T01:intron 25.0%
!!! TTCATCTAAGTTTTAGAGAT+TGG + Chr5:95364182-95364201 None:intergenic 25.0%
!!! TTTGAAAGAAGTTCAAGTTT+TGG + Chr5:95364099-95364118 None:intergenic 25.0%
!!! TTTGGAAATCACTTTTAAGT+TGG - Chr5:95360997-95361016 MsG0580029330.01.T01:three_prime_UTR 25.0%
!!! TTTGTGCCAATGTTTTAAAA+CGG + Chr5:95362323-95362342 None:intergenic 25.0%
AAGTGAATCTGTGAATAGAA+GGG - Chr5:95362469-95362488 MsG0580029330.01.T01:intron 30.0%
ACAAATTAAAGAACCAGTTC+AGG + Chr5:95362229-95362248 None:intergenic 30.0%
AGCGCAACAGTTAAATATTA+AGG + Chr5:95363432-95363451 None:intergenic 30.0%
ATAACATTGGAATGGAAACA+AGG + Chr5:95363168-95363187 None:intergenic 30.0%
ATACATGGAATTCCATTTAC+TGG + Chr5:95364680-95364699 None:intergenic 30.0%
CAATTACTTGAGTTATAGTC+AGG - Chr5:95364133-95364152 MsG0580029330.01.T01:intron 30.0%
CACCAAATATTAGTGTCTTT+GGG + Chr5:95363358-95363377 None:intergenic 30.0%
CACGTTATTATCAGAAAACA+TGG - Chr5:95364407-95364426 MsG0580029330.01.T01:intron 30.0%
CACTTATTAACTCGCATTTA+AGG + Chr5:95361848-95361867 None:intergenic 30.0%
CAGATGCTCAAGAAATAAAT+TGG + Chr5:95365086-95365105 None:intergenic 30.0%
CATAAGAAGCCAAAACATTA+TGG + Chr5:95362153-95362172 None:intergenic 30.0%
CTGACTATAACTCAAGTAAT+TGG + Chr5:95364135-95364154 None:intergenic 30.0%
GATCATAGAAAATAGTCTTC+TGG - Chr5:95362032-95362051 MsG0580029330.01.T01:intron 30.0%
GTAATATCTTCAAAGTCCTA+AGG - Chr5:95363934-95363953 MsG0580029330.01.T01:intron 30.0%
GTGTAATTTATCTCTCTTTC+TGG - Chr5:95363194-95363213 MsG0580029330.01.T01:intron 30.0%
TGGCAATCTACATACATAAA+TGG + Chr5:95365148-95365167 None:intergenic 30.0%
TGTTGTCTACAATTACAATG+CGG + Chr5:95364634-95364653 None:intergenic 30.0%
TTGAGTTATAGTCAGGTAAT+AGG - Chr5:95364140-95364159 MsG0580029330.01.T01:intron 30.0%
TTGTCTATTATGTCAACTAG+CGG + Chr5:95363084-95363103 None:intergenic 30.0%
TTTGTATTGCAACCAGTAAA+TGG - Chr5:95364665-95364684 MsG0580029330.01.T01:intron 30.0%
! AAAAGTGTAAATACAGGAGA+TGG + Chr5:95364814-95364833 None:intergenic 30.0%
! CAACATGGAAATTTTGTGTT+TGG - Chr5:95361544-95361563 MsG0580029330.01.T01:CDS 30.0%
! GCTGATAAAAGTGTAAATAC+AGG + Chr5:95364820-95364839 None:intergenic 30.0%
! GGAATCGATTATTGTTGTTA+TGG - Chr5:95360926-95360945 MsG0580029330.01.T01:three_prime_UTR 30.0%
! GTTGACTTCAATCATCTTAA+AGG - Chr5:95361948-95361967 MsG0580029330.01.T01:intron 30.0%
! GTTTCTTAGGTGAATTGTTT+TGG - Chr5:95361450-95361469 MsG0580029330.01.T01:intron 30.0%
! TGACAAACATTATTTTCAGC+TGG + Chr5:95362509-95362528 None:intergenic 30.0%
! TGGAATTTTTCATAGAGCAA+AGG + Chr5:95364899-95364918 None:intergenic 30.0%
! TGGTGTTAAGTTATTAGGTT+TGG - Chr5:95361890-95361909 MsG0580029330.01.T01:intron 30.0%
! TTTATTTCTTGAGCATCTGA+AGG - Chr5:95365087-95365106 MsG0580029330.01.T01:CDS 30.0%
!! AACAGTGAAGCTTAGTTTTT+AGG + Chr5:95362986-95363005 None:intergenic 30.0%
!! AATACCGTAAGTTTTTGGAA+AGG + Chr5:95361233-95361252 None:intergenic 30.0%
!! AGAACACAAAATTCTAGTTC+TGG + Chr5:95362811-95362830 None:intergenic 30.0%
!! ATACCGTAAGTTTTTGGAAA+GGG + Chr5:95361232-95361251 None:intergenic 30.0%
!! TATGGATTGGGATTTAATAG+AGG + Chr5:95364351-95364370 None:intergenic 30.0%
!! TGGTTTGAATTGGTTTGATA+TGG - Chr5:95362738-95362757 MsG0580029330.01.T01:intron 30.0%
!! TTAAGTTATTAGGTTTGGTG+TGG - Chr5:95361895-95361914 MsG0580029330.01.T01:intron 30.0%
!!! AATGTTTTGGCTTCTTATGA+TGG - Chr5:95362154-95362173 MsG0580029330.01.T01:intron 30.0%
!!! TTTTGGGTTTGTTATGAGTA+TGG - Chr5:95361467-95361486 MsG0580029330.01.T01:CDS 30.0%
AAACTGAAGTGTATGCTACA+TGG + Chr5:95361590-95361609 None:intergenic 35.0%
AAATACATACTAACACGCGT+TGG + Chr5:95361632-95361651 None:intergenic 35.0%
AACGAACAAAAAAGAGGTTG+AGG + Chr5:95363862-95363881 None:intergenic 35.0%
AATGAAACAAAAGAGCCTTG+CGG + Chr5:95364992-95365011 None:intergenic 35.0%
AGAAAATAGTCTTCTGGGTT+TGG - Chr5:95362038-95362057 MsG0580029330.01.T01:intron 35.0%
ATACAGACAGACATATGGAT+TGG + Chr5:95364364-95364383 None:intergenic 35.0%
ATCGAAATCCTGAAGTTCAA+CGG + Chr5:95361156-95361175 None:intergenic 35.0%
CACCCAAAGACACTAATATT+TGG - Chr5:95363353-95363372 MsG0580029330.01.T01:intron 35.0%
CTGGCTATAGATCAATAACA+CGG - Chr5:95364288-95364307 MsG0580029330.01.T01:intron 35.0%
GAAGTGAATCTGTGAATAGA+AGG - Chr5:95362468-95362487 MsG0580029330.01.T01:intron 35.0%
GATCTCATGTATTCACTTGT+AGG - Chr5:95362630-95362649 MsG0580029330.01.T01:intron 35.0%
GATGATGAAGAAGAGAGTAA+AGG + Chr5:95361077-95361096 None:intergenic 35.0%
GATTCAGTTTACGACTTTAC+CGG - Chr5:95363112-95363131 MsG0580029330.01.T01:intron 35.0%
GATTTAAATTGTTTGCCGCA+AGG - Chr5:95364974-95364993 MsG0580029330.01.T01:CDS 35.0%
GCACCAAATATTAGTGTCTT+TGG + Chr5:95363359-95363378 None:intergenic 35.0%
GCTAGTTGACATAATAGACA+AGG - Chr5:95363083-95363102 MsG0580029330.01.T01:CDS 35.0%
TAAATCCTACAATGTCCAAC+TGG + Chr5:95364960-95364979 None:intergenic 35.0%
TACAGACAGACATATGGATT+GGG + Chr5:95364363-95364382 None:intergenic 35.0%
TAGTCAGGTAATAGGTAACT+AGG - Chr5:95364148-95364167 MsG0580029330.01.T01:intron 35.0%
TCCAACTTATAGAGAAGATG+AGG + Chr5:95363298-95363317 None:intergenic 35.0%
TCCTCATCTTCTCTATAAGT+TGG - Chr5:95363294-95363313 MsG0580029330.01.T01:CDS 35.0%
TGGCTATAGATCAATAACAC+GGG - Chr5:95364289-95364308 MsG0580029330.01.T01:intron 35.0%
TTTCTTGTTCACTCAACACT+CGG - Chr5:95363833-95363852 MsG0580029330.01.T01:intron 35.0%
TTTGAGCTTGAAGTTTAGAG+TGG - Chr5:95364507-95364526 MsG0580029330.01.T01:intron 35.0%
TTTGCAAACGTATAACAGCT+CGG + Chr5:95362574-95362593 None:intergenic 35.0%
! AAGTTTAGAGTGGAGTCTAT+TGG - Chr5:95364517-95364536 MsG0580029330.01.T01:intron 35.0%
! CATGTATTCCATTTTCTGCT+GGG - Chr5:95364693-95364712 MsG0580029330.01.T01:intron 35.0%
! TCAAAAGTACGTAAACCCTT+AGG + Chr5:95363953-95363972 None:intergenic 35.0%
! TGCTCTGATGTTAGCTTATT+GGG - Chr5:95361328-95361347 MsG0580029330.01.T01:CDS 35.0%
! TTGCTCTGATGTTAGCTTAT+TGG - Chr5:95361327-95361346 MsG0580029330.01.T01:CDS 35.0%
!! CAAGGCTCTTTTGTTTCATT+TGG - Chr5:95364992-95365011 MsG0580029330.01.T01:CDS 35.0%
!! CTAGTTCTGGAAAAGCAATT+AGG + Chr5:95362798-95362817 None:intergenic 35.0%
!! GAATTTAATGAGAGTACTCG+AGG + Chr5:95364235-95364254 None:intergenic 35.0%
!! TTCAATGGATTAGTGTACCT+GGG + Chr5:95364732-95364751 None:intergenic 35.0%
!! TTTCAATGGATTAGTGTACC+TGG + Chr5:95364733-95364752 None:intergenic 35.0%
!!! GTCTGTCCGTTTTAAAACAT+TGG - Chr5:95362314-95362333 MsG0580029330.01.T01:intron 35.0%
AAAATCGGCGGTGATTTCAA+GGG + Chr5:95361192-95361211 None:intergenic 40.0%
AAATCAACACTGCAGCTCAA+TGG + Chr5:95362286-95362305 None:intergenic 40.0%
AAATCCTGAAGTTCAACGGA+AGG + Chr5:95361152-95361171 None:intergenic 40.0%
AACTTGAGTGCAAGTCTTGA+AGG - Chr5:95363791-95363810 MsG0580029330.01.T01:intron 40.0%
ACCACTCAACTCAATGAAGT+AGG + Chr5:95362093-95362112 None:intergenic 40.0%
ACCTACGTGCTAATTGTTGA+AGG + Chr5:95363138-95363157 None:intergenic 40.0%
ACGCCCTTTCCAAAAACTTA+CGG - Chr5:95361226-95361245 MsG0580029330.01.T01:CDS 40.0%
ACTCAAGTAATTGGGATGAG+TGG + Chr5:95364126-95364145 None:intergenic 40.0%
AGAGTAAAGGATAGTGACCA+AGG + Chr5:95361064-95361083 None:intergenic 40.0%
ATAATCTCGTGGTGCAATAG+AGG - Chr5:95363612-95363631 MsG0580029330.01.T01:intron 40.0%
ATATGCTTGCAGGGAACATA+GGG - Chr5:95364568-95364587 MsG0580029330.01.T01:intron 40.0%
CCAGCAGAAAATGGAATACA+TGG + Chr5:95364695-95364714 None:intergenic 40.0%
CTCAAGTAATTGGGATGAGT+GGG + Chr5:95364125-95364144 None:intergenic 40.0%
CTTGTAGGTAGTACTTGACT+TGG - Chr5:95362645-95362664 MsG0580029330.01.T01:intron 40.0%
GAGTAAAGGATAGTGACCAA+GGG + Chr5:95361063-95361082 None:intergenic 40.0%
GCTTCTGGTAAAACCAACAA+GGG + Chr5:95363997-95364016 None:intergenic 40.0%
GGAATGTGTATCTTCTGACA+AGG - Chr5:95362759-95362778 MsG0580029330.01.T01:intron 40.0%
GGTTGCAATACAAAGCTGAA+AGG + Chr5:95364659-95364678 None:intergenic 40.0%
GTAAGTGTTAGTGTTGTGTC+TGG - Chr5:95361659-95361678 MsG0580029330.01.T01:intron 40.0%
GTAATTCGTTGCTGATAGCT+GGG - Chr5:95363216-95363235 MsG0580029330.01.T01:intron 40.0%
GTTAAGAATTCCTTCAACCC+AGG + Chr5:95362907-95362926 None:intergenic 40.0%
TACATTGTTGTGTCCTGAAC+TGG - Chr5:95362213-95362232 MsG0580029330.01.T01:intron 40.0%
TATATGCTTGCAGGGAACAT+AGG - Chr5:95364567-95364586 MsG0580029330.01.T01:intron 40.0%
TCAAGTAATTGGGATGAGTG+GGG + Chr5:95364124-95364143 None:intergenic 40.0%
TCTATAGGCTTGCAATCTGT+TGG - Chr5:95365183-95365202 MsG0580029330.01.T01:CDS 40.0%
TCTCAGATTATTCGAACCCT+TGG - Chr5:95361044-95361063 MsG0580029330.01.T01:three_prime_UTR 40.0%
TTCAGTGGATGATAATCTCG+TGG - Chr5:95363460-95363479 MsG0580029330.01.T01:CDS 40.0%
TTGAGTGGATGATAATCTCG+TGG - Chr5:95363601-95363620 MsG0580029330.01.T01:intron 40.0%
TTGCTCTTTGCATAGTTGAG+TGG - Chr5:95363586-95363605 MsG0580029330.01.T01:intron 40.0%
! AGAGGTGAATCTGGATTAGA+CGG - Chr5:95364021-95364040 MsG0580029330.01.T01:intron 40.0%
! CCATGTATTCCATTTTCTGC+TGG - Chr5:95364692-95364711 MsG0580029330.01.T01:intron 40.0%
! TTAGGCGTGTTGGATTTTCA+AGG - Chr5:95362834-95362853 MsG0580029330.01.T01:intron 40.0%
!! ACCTACTTCATTGAGTTGAG+TGG - Chr5:95362089-95362108 MsG0580029330.01.T01:intron 40.0%
!! ACTTAGTGAATTTGCTGACC+TGG - Chr5:95362930-95362949 MsG0580029330.01.T01:intron 40.0%
!! ATAATCTCGTGGTGCTATAG+AGG - Chr5:95363471-95363490 MsG0580029330.01.T01:CDS 40.0%
!! CAATCGAAGCTGGTTTGAAT+TGG - Chr5:95362728-95362747 MsG0580029330.01.T01:intron 40.0%
!! CGCGTTGGTGTTAAGTTATT+AGG - Chr5:95361885-95361904 MsG0580029330.01.T01:intron 40.0%
!! TGGATTTTCAAGGAAGGAGA+AGG - Chr5:95362844-95362863 MsG0580029330.01.T01:intron 40.0%
!! TTCCAAACTTGTTGAGTTCG+TGG + Chr5:95360984-95361003 None:intergenic 40.0%
!!! TTTTTGGAAAGGGCGTTGAT+CGG + Chr5:95361222-95361241 None:intergenic 40.0%
ACGTGCTAATTGTTGAAGGC+CGG + Chr5:95363134-95363153 None:intergenic 45.0%
ACTCGAGGAGAACTAAAGAG+TGG + Chr5:95364220-95364239 None:intergenic 45.0%
AGCTTCCAGTTGGACATTGT+AGG - Chr5:95364952-95364971 MsG0580029330.01.T01:CDS 45.0%
CACCACGAACTCAACAAGTT+TGG - Chr5:95360979-95360998 MsG0580029330.01.T01:three_prime_UTR 45.0%
CGCTTCTGGTAAAACCAACA+AGG + Chr5:95363998-95364017 None:intergenic 45.0%
GAAAACATAGACGAACCAGC+TGG + Chr5:95364057-95364076 None:intergenic 45.0%
GAAAATCGGCGGTGATTTCA+AGG + Chr5:95361193-95361212 None:intergenic 45.0%
GCCTTCAACAATTAGCACGT+AGG - Chr5:95363134-95363153 MsG0580029330.01.T01:intron 45.0%
GGAACATAGGGAACATGTCA+AGG - Chr5:95364580-95364599 MsG0580029330.01.T01:intron 45.0%
GGATCTTACCTGCATATCGA+AGG - Chr5:95364601-95364620 MsG0580029330.01.T01:intron 45.0%
GGTAATTCGTTGCTGATAGC+TGG - Chr5:95363215-95363234 MsG0580029330.01.T01:intron 45.0%
GTCAAGATCCTTCGATATGC+AGG + Chr5:95364612-95364631 None:intergenic 45.0%
GTGTTCTCATTTAGGCGTGT+TGG - Chr5:95362824-95362843 MsG0580029330.01.T01:intron 45.0%
TACAATGTCCAACTGGAAGC+TGG + Chr5:95364953-95364972 None:intergenic 45.0%
TCAGAGAGTTGCTTGAGAAG+TGG + Chr5:95364860-95364879 None:intergenic 45.0%
TCTTCCTTCCGTTGAACTTC+AGG - Chr5:95361145-95361164 MsG0580029330.01.T01:three_prime_UTR 45.0%
TGAAGTTCAACGGAAGGAAG+AGG + Chr5:95361146-95361165 None:intergenic 45.0%
TGAGTGCAAGTCTTGAAGGA+TGG - Chr5:95363795-95363814 MsG0580029330.01.T01:intron 45.0%
TGGCTGGTGGAATTTGGTAA+TGG - Chr5:95361926-95361945 MsG0580029330.01.T01:intron 45.0%
TTACAGTCTTTGAACGGCCT+GGG - Chr5:95362887-95362906 MsG0580029330.01.T01:intron 45.0%
! ACATTATTTTCAGCTGGCGC+TGG + Chr5:95362503-95362522 None:intergenic 45.0%
! CTGATTGGTTCCAGAAGCAA+GGG - Chr5:95364924-95364943 MsG0580029330.01.T01:intron 45.0%
! TATGGGCGAGTTGACTCAAA+CGG - Chr5:95360944-95360963 MsG0580029330.01.T01:three_prime_UTR 45.0%
! TGGAAAGTGCAGATGCTGAT+GGG - Chr5:95365037-95365056 MsG0580029330.01.T01:CDS 45.0%
! TGTTGTTGGCTGGTGGAATT+TGG - Chr5:95361920-95361939 MsG0580029330.01.T01:intron 45.0%
!! CAAACTTGTTGAGTTCGTGG+TGG + Chr5:95360981-95361000 None:intergenic 45.0%
!! GCGTGTTGGATTTTCAAGGA+AGG - Chr5:95362838-95362857 MsG0580029330.01.T01:intron 45.0%
!!! GTTTTACCAGAAGCGCTTAG+AGG - Chr5:95364003-95364022 MsG0580029330.01.T01:intron 45.0%
AAGCTGGAATCCCTTGCTTC+TGG + Chr5:95364937-95364956 None:intergenic 50.0%
AAGGGATTCCAGCTTCCAGT+TGG - Chr5:95364942-95364961 MsG0580029330.01.T01:CDS 50.0%
CGAGGAGAACTAAAGAGTGG+AGG + Chr5:95364217-95364236 None:intergenic 50.0%
CGCGTTGGACACTAAACACA+TGG + Chr5:95361617-95361636 None:intergenic 50.0%
CGTTCTCAAGCAATCGAAGC+TGG - Chr5:95362718-95362737 MsG0580029330.01.T01:intron 50.0%
GAGTCGAGACCATTGAATCG+TGG - Chr5:95363316-95363335 MsG0580029330.01.T01:CDS 50.0%
GATTCACCTCTAAGCGCTTC+TGG + Chr5:95364012-95364031 None:intergenic 50.0%
GTTACAGTCTTTGAACGGCC+TGG - Chr5:95362886-95362905 MsG0580029330.01.T01:intron 50.0%
GTTGTGTCTGGTGTCTGTGT+CGG - Chr5:95361671-95361690 MsG0580029330.01.T01:intron 50.0%
TCAGTGCTGCCACGATTCAA+TGG + Chr5:95363328-95363347 None:intergenic 50.0%
TGTGGATGCCCAGCAGAAAA+TGG + Chr5:95364704-95364723 None:intergenic 50.0%
TTGCGCTTTGCGTAGTTCAG+TGG - Chr5:95363445-95363464 MsG0580029330.01.T01:CDS 50.0%
TTGTGTCTGGTGTCTGTGTC+GGG - Chr5:95361672-95361691 MsG0580029330.01.T01:intron 50.0%
! CTGGAAAGTGCAGATGCTGA+TGG - Chr5:95365036-95365055 MsG0580029330.01.T01:CDS 50.0%
! GAAGCGCTTAGAGGTGAATC+TGG - Chr5:95364012-95364031 MsG0580029330.01.T01:intron 50.0%
! TAGTGTACCTGGGTGTCATG+TGG + Chr5:95364722-95364741 None:intergenic 50.0%
! TTTTCTGAGTGTGTGCGCGT+TGG - Chr5:95361870-95361889 MsG0580029330.01.T01:intron 50.0%
!! GGTTTGGTGTGGTGTGTTGT+TGG - Chr5:95361906-95361925 MsG0580029330.01.T01:intron 50.0%
!! TGACCTGGTGCTGTTTACTG+CGG - Chr5:95362945-95362964 MsG0580029330.01.T01:intron 50.0%
ATCGGCGGTGATTTCAAGGG+CGG + Chr5:95361189-95361208 None:intergenic 55.0%
CTGTTTACTGCGGGTCTCGA+AGG - Chr5:95362955-95362974 MsG0580029330.01.T01:intron 55.0%
GACGGACAAAACTGTCCAGC+TGG - Chr5:95364039-95364058 MsG0580029330.01.T01:intron 55.0%
GCTTGAGAACGTAGTGCAGC+TGG + Chr5:95362709-95362728 None:intergenic 55.0%
TGTGTCTGGTGTCTGTGTCG+GGG - Chr5:95361673-95361692 MsG0580029330.01.T01:intron 55.0%
TTGATCGGCGGCGGGAAAAT+CGG + Chr5:95361207-95361226 None:intergenic 55.0%
! CTTTGAACGGCCTGGGTTGA+AGG - Chr5:95362894-95362913 MsG0580029330.01.T01:intron 55.0%
! TGGTGTGGTGTGTTGTTGGC+TGG - Chr5:95361910-95361929 MsG0580029330.01.T01:intron 55.0%
! TGTGGTGTGTTGTTGGCTGG+TGG - Chr5:95361913-95361932 MsG0580029330.01.T01:intron 55.0%
!! GACCTGGTGCTGTTTACTGC+GGG - Chr5:95362946-95362965 MsG0580029330.01.T01:intron 55.0%
!! TTGGAAAGGGCGTTGATCGG+CGG + Chr5:95361219-95361238 None:intergenic 55.0%
GACCCGCAGTAAACAGCACC+AGG + Chr5:95362951-95362970 None:intergenic 60.0%
GATTTCAAGGGCGGACGAGG+AGG + Chr5:95361180-95361199 None:intergenic 60.0%
GGTGATTTCAAGGGCGGACG+AGG + Chr5:95361183-95361202 None:intergenic 60.0%
TGGGCATCCACATGACACCC+AGG - Chr5:95364712-95364731 MsG0580029330.01.T01:intron 60.0%
! GGGCGAGTTGACTCAAACGG+AGG - Chr5:95360947-95360966 MsG0580029330.01.T01:three_prime_UTR 60.0%
ATCGGCGGCGGGAAAATCGG+CGG + Chr5:95361204-95361223 None:intergenic 65.0%
!! AAAGGGCGTTGATCGGCGGC+GGG + Chr5:95361215-95361234 None:intergenic 65.0%
!! GAAAGGGCGTTGATCGGCGG+CGG + Chr5:95361216-95361235 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 95360924 95365272 95360924 ID=MsG0580029330.01;Name=MsG0580029330.01
Chr5 mRNA 95360924 95365272 95360924 ID=MsG0580029330.01.T01;Parent=MsG0580029330.01;Name=MsG0580029330.01.T01;_AED=0.39;_eAED=0.41;_QI=0|0.6|0.66|1|0.8|0.83|6|299|326
Chr5 exon 95360924 95361357 95360924 ID=MsG0580029330.01.T01:exon:12180;Parent=MsG0580029330.01.T01
Chr5 exon 95361463 95361616 95361463 ID=MsG0580029330.01.T01:exon:12179;Parent=MsG0580029330.01.T01
Chr5 exon 95363045 95363126 95363045 ID=MsG0580029330.01.T01:exon:12178;Parent=MsG0580029330.01.T01
Chr5 exon 95363220 95363358 95363220 ID=MsG0580029330.01.T01:exon:12177;Parent=MsG0580029330.01.T01
Chr5 exon 95363416 95363544 95363416 ID=MsG0580029330.01.T01:exon:12176;Parent=MsG0580029330.01.T01
Chr5 exon 95364931 95365272 95364931 ID=MsG0580029330.01.T01:exon:12175;Parent=MsG0580029330.01.T01
Chr5 CDS 95364931 95365272 95364931 ID=MsG0580029330.01.T01:cds;Parent=MsG0580029330.01.T01
Chr5 CDS 95363416 95363544 95363416 ID=MsG0580029330.01.T01:cds;Parent=MsG0580029330.01.T01
Chr5 CDS 95363220 95363358 95363220 ID=MsG0580029330.01.T01:cds;Parent=MsG0580029330.01.T01
Chr5 CDS 95363045 95363126 95363045 ID=MsG0580029330.01.T01:cds;Parent=MsG0580029330.01.T01
Chr5 CDS 95361463 95361616 95361463 ID=MsG0580029330.01.T01:cds;Parent=MsG0580029330.01.T01
Chr5 CDS 95361223 95361357 95361223 ID=MsG0580029330.01.T01:cds;Parent=MsG0580029330.01.T01
Chr5 three_prime_UTR 95360924 95361222 95360924 ID=MsG0580029330.01.T01:three_prime_utr;Parent=MsG0580029330.01.T01
Gene Sequence

>MsG0580029330.01.T01

TTGGAATCGATTATTGTTGTTATGGGCGAGTTGACTCAAACGGAGGTGTACTCGCCACCACGAACTCAACAAGTTTGGAAATCACTTTTAAGTTGGTTCGCTTTTTTCTTTCAGATCTTCTCTCAGATTATTCGAACCCTTGGTCACTATCCTTTACTCTCTTCTTCATCATCTTCTTCTTCTTCTTCTTCGTCTTCTTCAACTTCTTCTTCTTTCAAACCTCTTCCTTCCGTTGAACTTCAGGATTTCGATTCCTCCTCGTCCGCCCTTGAAATCACCGCCGATTTTCCCGCCGCCGATCAACGCCCTTTCCAAAAACTTACGGTATTAAATATGTTTTTAGTAGTACTTGACTTGGATGAAACACTTGTATGTGCATACGAGACATCAAGTCTGCCAGCTGCACTACGTTCTCAAGCAATCGAAGCTGGTTTGAATTGGTTTGATATGGAATGTGTATCTTCTGACAAGGCGTGTTGGATTTTCAAGGAAGGAGAAGGAAGACCAAAAATTAATTATGTTACAGTCTTTGAACGGCCTGGGTTGAAGGAATTCTTAACGAAACTTAGTGAATTTGCTGACCTGGTGCTGTTTACTGCGGGTCTCGAAGGCTATGCTAGACCGCTAGTTGACATAATAGACAAGGAAAATCGATTCAGTTTACGACTTTACCGGCCTTCAACAATTAGCACGGAACATAGGGAACATGTCAAGGATCTTACCTGCATATCGAAGGATCTTGACCGCATTGTAATTGTAGACAACAATCCTTTCAGCTTTGTATTGCAACCAGTAAATGGAATTCCATGTATTCCATTTTCTGCTGGGCATCCACATGACACCCAGCTTCTAGATGTCATTCTTCCACTTCTCAAGCAACTCTCTGAGCAGAATGATGTAAGACCTTTGCTCTATGAAAAATTCCACATGACTGATTGGTTCCAGAAGCAAGGGATTCCAGCTTCCAGTTGGACATTGTAG
Protein sequence

>MsG0580029330.01.T01

LESIIVVMGELTQTEVYSPPRTQQVWKSLLSWFAFFFQIFSQIIRTLGHYPLLSSSSSSSSSSSSSSTSSSFKPLPSVELQDFDSSSSALEITADFPAADQRPFQKLTVLNMFLVVLDLDETLVCAYETSSLPAALRSQAIEAGLNWFDMECVSSDKACWIFKEGEGRPKINYVTVFERPGLKEFLTKLSEFADLVLFTAGLEGYARPLVDIIDKENRFSLRLYRPSTISTEHREHVKDLTCISKDLDRIVIVDNNPFSFVLQPVNGIPCIPFSAGHPHDTQLLDVILPLLKQLSEQNDVRPLLYEKFHMTDWFQKQGIPASSWTL*