AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029404.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029404.01.T01 MTR_5g085580 96.667 60 1 1 37 95 232 291 9.43e-31 113
MsG0580029404.01.T01 MTR_3g080410 76.667 60 13 1 37 95 233 292 8.15e-24 93.6
MsG0580029404.01.T01 MTR_3g116510 66.102 59 19 1 39 96 103 161 1.17e-20 84.3
MsG0580029404.01.T01 MTR_3g116510 66.102 59 19 1 39 96 237 295 2.36e-20 84.7
MsG0580029404.01.T01 MTR_4g069940 64.407 59 20 1 39 96 238 296 1.56e-19 82.4
MsG0580029404.01.T01 MTR_1g056640 65.000 60 20 1 37 95 245 304 3.44e-19 81.3
MsG0580029404.01.T01 MTR_1g022160 54.839 62 27 1 36 96 231 292 5.21e-19 80.5
MsG0580029404.01.T01 MTR_1g022160 54.839 62 27 1 36 96 231 292 1.20e-18 79.7
MsG0580029404.01.T01 MTR_1g022160 54.839 62 27 1 36 96 231 292 1.34e-18 79.7
MsG0580029404.01.T01 MTR_1g022160 54.839 62 27 1 36 96 231 292 1.59e-18 79.3
MsG0580029404.01.T01 MTR_1g077990 58.730 63 25 1 34 95 234 296 8.95e-18 77.0
MsG0580029404.01.T01 MTR_4g083500 50.820 61 29 1 37 96 250 310 8.75e-17 74.7
MsG0580029404.01.T01 MTR_3g065460 55.738 61 26 1 37 96 233 293 1.13e-16 74.3
MsG0580029404.01.T01 MTR_4g010200 47.887 71 36 1 24 93 227 297 8.09e-16 71.6
MsG0580029404.01.T01 MTR_2g086530 56.667 60 25 1 37 95 233 292 3.29e-15 70.1
MsG0580029404.01.T01 MTR_2g086530 56.667 60 25 1 37 95 212 271 3.47e-15 69.7
MsG0580029404.01.T01 MTR_8g012400 46.377 69 36 1 37 104 157 225 4.69e-15 69.3
MsG0580029404.01.T01 MTR_8g012400 46.377 69 36 1 37 104 237 305 4.70e-15 69.7
MsG0580029404.01.T01 MTR_6g088885 52.459 61 28 1 37 96 233 293 7.56e-15 68.9
MsG0580029404.01.T01 MTR_5g078200 50.820 61 29 1 37 96 232 292 1.05e-14 68.6
MsG0580029404.01.T01 MTR_5g078200 50.820 61 29 1 37 96 199 259 1.16e-14 68.6
MsG0580029404.01.T01 MTR_4g088155 47.826 69 35 1 37 104 233 301 1.93e-14 67.8
MsG0580029404.01.T01 MTR_4g052970 53.333 60 27 1 37 95 235 294 1.98e-14 67.8
MsG0580029404.01.T01 MTR_1g007810 43.548 62 34 1 36 96 219 280 2.39e-14 67.4
MsG0580029404.01.T01 MTR_8g102340 52.459 61 28 1 37 96 200 260 4.81e-14 66.6
MsG0580029404.01.T01 MTR_7g053500 50.000 60 29 1 37 95 157 216 9.11e-14 65.9
MsG0580029404.01.T01 MTR_7g053500 50.000 60 29 1 37 95 234 293 9.45e-14 65.9
MsG0580029404.01.T01 MTR_4g084480 53.333 60 27 1 37 95 231 290 1.01e-13 65.9
MsG0580029404.01.T01 MTR_4g084480 53.333 60 27 1 37 95 238 297 1.29e-13 65.5
MsG0580029404.01.T01 MTR_4g084480 53.333 60 27 1 37 95 238 297 1.33e-13 65.5
MsG0580029404.01.T01 MTR_4g114850 54.237 59 26 1 36 93 232 290 1.42e-13 65.5
MsG0580029404.01.T01 MTR_4g114850 54.237 59 26 1 36 93 232 290 1.66e-13 65.1
MsG0580029404.01.T01 MTR_4g114850 54.237 59 26 1 36 93 232 290 1.71e-13 65.1
MsG0580029404.01.T01 MTR_1g031900 49.180 61 30 1 37 96 233 293 2.15e-13 64.7
MsG0580029404.01.T01 MTR_1g031900 49.180 61 30 1 37 96 233 293 3.69e-13 64.3
MsG0580029404.01.T01 MTR_1g052430 42.683 82 43 2 22 102 222 300 5.28e-13 63.5
MsG0580029404.01.T01 MTR_3g083580 46.667 60 31 1 37 95 235 294 8.36e-13 63.2
MsG0580029404.01.T01 MTR_2g019345 53.333 60 27 1 37 95 229 288 1.18e-12 62.8
MsG0580029404.01.T01 MTR_4g132280 45.902 61 32 1 37 96 254 314 3.19e-12 61.6
MsG0580029404.01.T01 MTR_8g085720 49.153 59 29 1 38 95 236 294 3.78e-12 61.2
MsG0580029404.01.T01 MTR_7g081350 44.262 61 31 2 37 96 228 286 9.71e-12 60.1
MsG0580029404.01.T01 MTR_4g076470 47.692 65 30 2 39 102 224 285 1.04e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029404.01.T01 AT2G05790 73.333 60 15 1 37 95 232 291 1.08e-23 94.0
MsG0580029404.01.T01 AT5G42720 55.556 63 27 1 37 98 234 296 5.31e-19 80.9
MsG0580029404.01.T01 AT2G16230 57.377 61 25 1 37 96 232 292 5.69e-19 80.9
MsG0580029404.01.T01 AT4G34480 55.738 61 26 1 37 96 233 293 1.56e-18 79.0
MsG0580029404.01.T01 AT4G34480 55.738 61 26 1 37 96 122 182 2.48e-18 78.6
MsG0580029404.01.T01 AT4G34480 55.738 61 26 1 37 96 233 293 2.94e-18 79.0
MsG0580029404.01.T01 AT4G26830 65.000 60 20 1 37 95 235 294 4.23e-18 78.2
MsG0580029404.01.T01 AT4G26830 65.000 60 20 1 37 95 231 290 4.83e-18 78.2
MsG0580029404.01.T01 AT3G46570 51.613 62 29 1 36 96 232 293 7.24e-18 77.0
MsG0580029404.01.T01 AT5G55180 61.667 60 22 1 37 95 234 293 2.11e-17 76.3
MsG0580029404.01.T01 AT1G30080 60.656 61 23 1 36 95 241 301 2.38e-17 75.9
MsG0580029404.01.T01 AT5G55180 61.667 60 22 1 37 95 234 293 2.69e-17 75.9
MsG0580029404.01.T01 AT1G30080 60.656 61 23 1 36 95 241 301 2.70e-17 75.9
MsG0580029404.01.T01 AT2G27500 58.333 60 24 1 37 95 158 217 8.47e-17 73.9
MsG0580029404.01.T01 AT2G27500 58.333 60 24 1 37 95 236 295 1.42e-16 73.9
MsG0580029404.01.T01 AT2G27500 58.333 60 24 1 37 95 236 295 1.51e-16 73.6
MsG0580029404.01.T01 AT3G15800 55.738 61 26 1 34 93 248 308 2.81e-15 70.1
MsG0580029404.01.T01 AT2G01630 52.459 61 28 1 37 96 232 292 2.10e-14 67.8
MsG0580029404.01.T01 AT1G32860 53.333 60 27 1 37 95 235 294 2.15e-14 67.8
MsG0580029404.01.T01 AT2G01630 52.459 61 28 1 37 96 232 292 2.20e-14 67.8
MsG0580029404.01.T01 AT4G29360 49.180 61 30 1 37 96 243 303 2.40e-14 67.8
MsG0580029404.01.T01 AT2G01630 52.459 61 28 1 37 96 232 292 2.42e-14 67.8
MsG0580029404.01.T01 AT5G24318 43.333 60 33 1 36 94 233 292 2.54e-14 67.4
MsG0580029404.01.T01 AT4G29360 49.180 61 30 1 37 96 233 293 2.59e-14 67.4
MsG0580029404.01.T01 AT5G24318 43.333 60 33 1 36 94 233 292 4.22e-14 67.0
MsG0580029404.01.T01 AT3G23770 40.244 82 48 1 24 104 234 315 4.88e-14 66.6
MsG0580029404.01.T01 AT4G18340 58.333 60 24 1 36 94 239 298 5.39e-14 66.6
MsG0580029404.01.T01 AT5G24318 43.333 60 33 1 36 94 233 292 5.83e-14 66.6
MsG0580029404.01.T01 AT5G24318 43.333 60 33 1 36 94 94 153 7.53e-14 65.9
MsG0580029404.01.T01 AT3G07320 47.826 69 35 1 37 104 233 301 1.01e-13 65.9
MsG0580029404.01.T01 AT4G18340 58.333 60 24 1 36 94 239 298 1.21e-13 65.5
MsG0580029404.01.T01 AT1G66250 47.541 61 31 1 37 96 241 301 2.84e-13 64.7
MsG0580029404.01.T01 AT1G11820 44.262 61 33 1 37 96 253 313 3.83e-13 64.3
MsG0580029404.01.T01 AT1G11820 44.262 61 33 1 37 96 253 313 5.30e-13 63.9
MsG0580029404.01.T01 AT1G11820 44.262 61 33 1 37 96 253 313 5.46e-13 63.9
MsG0580029404.01.T01 AT4G14080 31.579 133 60 3 3 104 185 317 2.05e-12 62.0
MsG0580029404.01.T01 AT5G42100 48.387 62 29 1 37 95 233 294 3.14e-12 61.6
MsG0580029404.01.T01 AT5G42100 48.387 62 29 1 37 95 233 294 3.24e-12 61.6
MsG0580029404.01.T01 AT2G26600 47.541 61 31 1 34 93 144 204 8.74e-12 60.1
MsG0580029404.01.T01 AT3G57260 50.769 65 28 2 39 102 250 311 9.15e-12 60.1
MsG0580029404.01.T01 AT3G57260 50.769 65 28 2 39 102 234 295 1.41e-11 59.7
MsG0580029404.01.T01 AT2G26600 47.541 61 31 1 34 93 238 298 1.76e-11 59.3
MsG0580029404.01.T01 AT3G13560 45.000 60 32 1 37 95 235 294 1.99e-11 59.3
MsG0580029404.01.T01 AT3G13560 45.000 60 32 1 37 95 235 294 1.99e-11 59.3
MsG0580029404.01.T01 AT3G13560 45.000 60 32 1 37 95 235 294 1.99e-11 59.3
MsG0580029404.01.T01 AT3G13560 45.000 60 32 1 37 95 235 294 1.99e-11 59.3
MsG0580029404.01.T01 AT3G13560 45.000 60 32 1 37 95 235 294 1.99e-11 59.3
MsG0580029404.01.T01 AT3G61810 47.458 59 30 1 39 96 268 326 8.47e-11 57.4

Find 33 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATATATATTACTTAAATT+TGG 0.144347 5:+96587030 None:intergenic
ACAAAGTGGGTCCCACTTAC+AGG 0.303756 5:-96589426 MsG0580029404.01.T01:intron
CGATATAAACGTCGTCGTTT+CGG 0.332732 5:-96588493 MsG0580029404.01.T01:CDS
AACGTCGTCGTTTCGGAAAC+GGG 0.333129 5:-96588486 MsG0580029404.01.T01:CDS
ACAGAAGGACGAGTAATAAT+AGG 0.344881 5:+96589457 None:intergenic
AAACGTCGTCGTTTCGGAAA+CGG 0.380698 5:-96588487 MsG0580029404.01.T01:CDS
GAATATAGCTATAGGTCATT+TGG 0.386718 5:-96587315 MsG0580029404.01.T01:three_prime_UTR
CGTCGATTTGTGCGTCGAAA+AGG 0.406146 5:+96588547 None:intergenic
TGTTATTGAGTTGGTGGAAG+TGG 0.411662 5:-96587216 MsG0580029404.01.T01:three_prime_UTR
TTAGTCTCTAAAAGTAAAGT+TGG 0.418175 5:+96587188 None:intergenic
GGGTCAGGTGGATGCGGGTA+AGG 0.418663 5:-96588584 MsG0580029404.01.T01:intron
ACTTCCACCAACTCAATAAC+AGG 0.421821 5:+96587218 None:intergenic
GCTAGAAAGAATATAGCTAT+AGG 0.460223 5:-96587323 MsG0580029404.01.T01:three_prime_UTR
GGTAAATGTGAATTTCCAAC+CGG 0.506534 5:-96587418 MsG0580029404.01.T01:CDS
TACTAGTAAAATCTGAAGAA+AGG 0.510243 5:-96587394 MsG0580029404.01.T01:exon
AAACCCGGGTCAGGTGGATG+CGG 0.527345 5:-96588590 MsG0580029404.01.T01:intron
AACCCGGGTCAGGTGGATGC+GGG 0.531806 5:-96588589 MsG0580029404.01.T01:intron
CTGAAGAAAGGACTGTGTAA+AGG 0.543170 5:-96587382 MsG0580029404.01.T01:three_prime_UTR
TTGACCTGTTATTGAGTTGG+TGG 0.543555 5:-96587222 MsG0580029404.01.T01:three_prime_UTR
AAGTTGGTGGAGTATCTTGA+AGG 0.548481 5:-96587352 MsG0580029404.01.T01:three_prime_UTR
AATTTGACCTGTTATTGAGT+TGG 0.560205 5:-96587225 MsG0580029404.01.T01:three_prime_UTR
GTGTAAAGGTTACAAGAAGT+TGG 0.563863 5:-96587368 MsG0580029404.01.T01:three_prime_UTR
TCATTACCATCACCTTTCGA+AGG 0.568639 5:+96588459 None:intergenic
GAAACGGGTTGGCCTTCGAA+AGG 0.578195 5:-96588471 MsG0580029404.01.T01:CDS
ACCTCGTGTTGTAAAAGACT+CGG 0.584347 5:+96589485 None:intergenic
GGTTGGCCTTCGAAAGGTGA+TGG 0.601053 5:-96588465 MsG0580029404.01.T01:CDS
TAAAGGTTACAAGAAGTTGG+TGG 0.601092 5:-96587365 MsG0580029404.01.T01:three_prime_UTR
GTTATTGAGTTGGTGGAAGT+GGG 0.604081 5:-96587215 MsG0580029404.01.T01:three_prime_UTR
GGTGTGGTCAGACCTGTAAG+TGG 0.609366 5:+96589414 None:intergenic
TCGTCGTTTCGGAAACGGGT+TGG 0.611751 5:-96588482 MsG0580029404.01.T01:CDS
GAAAGGTGATGGTAATGAAG+TGG 0.650157 5:-96588454 MsG0580029404.01.T01:CDS
GTGTGGTCAGACCTGTAAGT+GGG 0.657042 5:+96589415 None:intergenic
AAAGGTGATGGTAATGAAGT+GGG 0.661071 5:-96588453 MsG0580029404.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACAAAAAACAATGAGAAA+AGG + Chr5:96588668-96588687 None:intergenic 20.0%
!! TAATCTTCATAATGATATCA+AGG - Chr5:96587712-96587731 MsG0580029404.01.T01:intron 20.0%
!! TTTGTTAGATTCTTGTAATA+AGG - Chr5:96588681-96588700 MsG0580029404.01.T01:intron 20.0%
!!! ACTTACTAGTATTTTGATTA+CGG - Chr5:96589400-96589419 MsG0580029404.01.T01:intron 20.0%
!!! ATTCTTTTATGATATTGACA+TGG - Chr5:96588783-96588802 MsG0580029404.01.T01:intron 20.0%
!!! CTTTTAATGAAAAAAAATCC+CGG - Chr5:96588212-96588231 MsG0580029404.01.T01:intron 20.0%
!!! TAAAAGTGCGAATAAAAAAA+CGG + Chr5:96588198-96588217 None:intergenic 20.0%
!!! TAAATTGCATGTAAGTTTTT+AGG - Chr5:96589367-96589386 MsG0580029404.01.T01:intron 20.0%
!!! TGCTTTTATTATTGTTACTA+GGG - Chr5:96588892-96588911 MsG0580029404.01.T01:intron 20.0%
!!! TTGCTTTTATTATTGTTACT+AGG - Chr5:96588891-96588910 MsG0580029404.01.T01:intron 20.0%
!!! TTTTAATGAAAAAAAATCCC+GGG - Chr5:96588213-96588232 MsG0580029404.01.T01:intron 20.0%
! ACTTTGTAACAAAAAACAGA+AGG + Chr5:96587091-96587110 None:intergenic 25.0%
! ATTGTATACTTTCTTCTCAT+CGG + Chr5:96588270-96588289 None:intergenic 25.0%
! CAATAGTATTATCAGAAAAC+AGG - Chr5:96588563-96588582 MsG0580029404.01.T01:CDS 25.0%
! GAAAAAAGTCACTGATAAAT+AGG + Chr5:96588992-96589011 None:intergenic 25.0%
! GTGTATTTATCTGTGATTAA+TGG + Chr5:96587159-96587178 None:intergenic 25.0%
! TAAAATGAGCTTGTAGTATA+TGG - Chr5:96588725-96588744 MsG0580029404.01.T01:intron 25.0%
! TACTAGTAAAATCTGAAGAA+AGG - Chr5:96589136-96589155 MsG0580029404.01.T01:intron 25.0%
!! ACTAAGTTTTACTTTGTAGA+TGG - Chr5:96589453-96589472 MsG0580029404.01.T01:CDS 25.0%
!! GTTCTAAATTAGTCAAAGAT+TGG - Chr5:96588947-96588966 MsG0580029404.01.T01:intron 25.0%
!! TTAGTCTCTAAAAGTAAAGT+TGG + Chr5:96589345-96589364 None:intergenic 25.0%
!! TTTAGGGTTAATTTACTTGA+GGG - Chr5:96588760-96588779 MsG0580029404.01.T01:intron 25.0%
!!! GATCTGTAAATGTTTTTTCA+TGG + Chr5:96587680-96587699 None:intergenic 25.0%
!!! TCTGTTTTTTGTTACAAAGT+GGG - Chr5:96587091-96587110 MsG0580029404.01.T01:three_prime_UTR 25.0%
!!! TTCTGTTTTTTGTTACAAAG+TGG - Chr5:96587090-96587109 MsG0580029404.01.T01:three_prime_UTR 25.0%
!!! TTTTAGGGTTAATTTACTTG+AGG - Chr5:96588759-96588778 MsG0580029404.01.T01:intron 25.0%
AATTTGACCTGTTATTGAGT+TGG - Chr5:96589305-96589324 MsG0580029404.01.T01:intron 30.0%
ACAAGAGTAATTGGAATTGT+TGG - Chr5:96588915-96588934 MsG0580029404.01.T01:intron 30.0%
ATCAAGCATGGGTTTAAAAA+CGG + Chr5:96587817-96587836 None:intergenic 30.0%
CTACTTCAGAAAGAAACTTT+GGG - Chr5:96588237-96588256 MsG0580029404.01.T01:intron 30.0%
GAATAGAAACAGAGGATTTA+GGG + Chr5:96587254-96587273 None:intergenic 30.0%
GAATATAGCTATAGGTCATT+TGG - Chr5:96589215-96589234 MsG0580029404.01.T01:intron 30.0%
GCTAGAAAGAATATAGCTAT+AGG - Chr5:96589207-96589226 MsG0580029404.01.T01:intron 30.0%
GCTATCAATGTACAAACTAT+AGG + Chr5:96588865-96588884 None:intergenic 30.0%
GTAAGATCTTGACATCAAAA+GGG - Chr5:96588144-96588163 MsG0580029404.01.T01:intron 30.0%
GTGATTATAGAATGCTTACT+AGG + Chr5:96588646-96588665 None:intergenic 30.0%
GTTAACCATTAGATAAGATC+CGG + Chr5:96587853-96587872 None:intergenic 30.0%
GTTTGACTCATAAGCAAAAA+CGG + Chr5:96587882-96587901 None:intergenic 30.0%
TGAATAGAAACAGAGGATTT+AGG + Chr5:96587255-96587274 None:intergenic 30.0%
TTTGACTCATAAGCAAAAAC+GGG + Chr5:96587881-96587900 None:intergenic 30.0%
! ATAGTTTGTACATTGATAGC+TGG - Chr5:96588865-96588884 MsG0580029404.01.T01:intron 30.0%
! CTTCAGATTTTACTAGTATC+CGG + Chr5:96589134-96589153 None:intergenic 30.0%
! CTTTTGATGTCAAGATCTTA+CGG + Chr5:96588145-96588164 None:intergenic 30.0%
! TGTTAATTTTGTGCAGAGTA+TGG - Chr5:96589091-96589110 MsG0580029404.01.T01:intron 30.0%
!! ATCTGTGATTAATGGATTGA+GGG + Chr5:96587151-96587170 None:intergenic 30.0%
!! TAAAAACGGGTTGAATAACT+CGG + Chr5:96587803-96587822 None:intergenic 30.0%
!! TATCTGTGATTAATGGATTG+AGG + Chr5:96587152-96587171 None:intergenic 30.0%
!!! AGCTTGTAGCTTAGTTTTAT+CGG + Chr5:96588439-96588458 None:intergenic 30.0%
!!! CTGTAAATGTTTTTTCATGG+CGG + Chr5:96587677-96587696 None:intergenic 30.0%
!!! GATTGGTTTTGTGTTAACAT+TGG - Chr5:96588964-96588983 MsG0580029404.01.T01:intron 30.0%
!!! GTAAGTTTTTAGGTTTGTCT+TGG - Chr5:96589377-96589396 MsG0580029404.01.T01:intron 30.0%
!!! GTATTTTGATTACGGTAGTA+AGG - Chr5:96589408-96589427 MsG0580029404.01.T01:intron 30.0%
!!! TGTAAATGTTTTTTCATGGC+GGG + Chr5:96587676-96587695 None:intergenic 30.0%
AAACGCTGATGTTATCTCAT+TGG - Chr5:96587898-96587917 MsG0580029404.01.T01:intron 35.0%
AACAGCAATGGCTTCTATAA+TGG + Chr5:96587605-96587624 None:intergenic 35.0%
AACTTCATTTCCAACAGCAA+TGG + Chr5:96587617-96587636 None:intergenic 35.0%
AAGTTTGTGTTAAGAGGATG+TGG + Chr5:96587214-96587233 None:intergenic 35.0%
ACAGAAGGACGAGTAATAAT+AGG + Chr5:96587076-96587095 None:intergenic 35.0%
AGATGGAAGTTTGTGTTAAG+AGG + Chr5:96587220-96587239 None:intergenic 35.0%
ATAATGCACGTAGAACTGAT+GGG + Chr5:96587463-96587482 None:intergenic 35.0%
ATGGCTTCTATAATGGTGTT+CGG + Chr5:96587598-96587617 None:intergenic 35.0%
ATGTATTAGGATCAAACACG+TGG + Chr5:96588519-96588538 None:intergenic 35.0%
ATTATAGAAGCCATTGCTGT+TGG - Chr5:96587604-96587623 MsG0580029404.01.T01:intron 35.0%
CCTACTTCAGAAAGAAACTT+TGG - Chr5:96588236-96588255 MsG0580029404.01.T01:intron 35.0%
CGTAAGATCTTGACATCAAA+AGG - Chr5:96588143-96588162 MsG0580029404.01.T01:intron 35.0%
CGTGTTTGATCCTAATACAT+TGG - Chr5:96588519-96588538 MsG0580029404.01.T01:CDS 35.0%
GGAGCATGTTGTATAATGTA+GGG - Chr5:96588838-96588857 MsG0580029404.01.T01:intron 35.0%
GGTAAATGTGAATTTCCAAC+CGG - Chr5:96589112-96589131 MsG0580029404.01.T01:intron 35.0%
GTGTAAAGGTTACAAGAAGT+TGG - Chr5:96589162-96589181 MsG0580029404.01.T01:intron 35.0%
GTTCAACTCCTAAAATCACA+AGG - Chr5:96587407-96587426 MsG0580029404.01.T01:exon 35.0%
GTTTCTGTGAATAGAAACAG+AGG + Chr5:96587262-96587281 None:intergenic 35.0%
GTTTGATCCTAATACATTGG+TGG - Chr5:96588522-96588541 MsG0580029404.01.T01:CDS 35.0%
TAAAGGTTACAAGAAGTTGG+TGG - Chr5:96589165-96589184 MsG0580029404.01.T01:intron 35.0%
TATACAATGTTCCTTAACCG+TGG - Chr5:96588282-96588301 MsG0580029404.01.T01:intron 35.0%
TCAAGCATGGGTTTAAAAAC+GGG + Chr5:96587816-96587835 None:intergenic 35.0%
TCACGAAGAAAATCAAGCAT+GGG + Chr5:96587828-96587847 None:intergenic 35.0%
TGAAACCGGATCTTATCTAA+TGG - Chr5:96587845-96587864 MsG0580029404.01.T01:intron 35.0%
TGACATGGCGTAACTTTATT+GGG - Chr5:96588798-96588817 MsG0580029404.01.T01:intron 35.0%
TGGAGCATGTTGTATAATGT+AGG - Chr5:96588837-96588856 MsG0580029404.01.T01:intron 35.0%
TTACTAGGGACAAGAGTAAT+TGG - Chr5:96588906-96588925 MsG0580029404.01.T01:intron 35.0%
TTAGGGTTTGGAAATGTAGA+TGG + Chr5:96587237-96587256 None:intergenic 35.0%
TTCACGAAGAAAATCAAGCA+TGG + Chr5:96587829-96587848 None:intergenic 35.0%
TTGACATGGCGTAACTTTAT+TGG - Chr5:96588797-96588816 MsG0580029404.01.T01:intron 35.0%
! AAAGGTGATGGTAATGAAGT+GGG - Chr5:96588077-96588096 MsG0580029404.01.T01:intron 35.0%
! AGATTTTACTAGTATCCGGT+TGG + Chr5:96589130-96589149 None:intergenic 35.0%
! ATGGTAAGCACGTGATTTTA+GGG - Chr5:96588744-96588763 MsG0580029404.01.T01:intron 35.0%
! CTTGATTTTCTTCGTGAAAC+CGG - Chr5:96587831-96587850 MsG0580029404.01.T01:intron 35.0%
! GGGTTATACCTTGTGATTTT+AGG + Chr5:96587418-96587437 None:intergenic 35.0%
! TATGGTAAGCACGTGATTTT+AGG - Chr5:96588743-96588762 MsG0580029404.01.T01:intron 35.0%
! TCAATCTTTTGCACTCTCAT+GGG - Chr5:96587546-96587565 MsG0580029404.01.T01:intron 35.0%
! TGGGTAGAGTGTACTATTTT+TGG - Chr5:96588817-96588836 MsG0580029404.01.T01:intron 35.0%
!! ATCGACAGTGAGTTTGATTT+TGG + Chr5:96587492-96587511 None:intergenic 35.0%
!! CCAAAGTTTCTTTCTGAAGT+AGG + Chr5:96588239-96588258 None:intergenic 35.0%
!! GTAATTTTTCAAAGCCTCCA+CGG + Chr5:96588302-96588321 None:intergenic 35.0%
!! TCTGTGATTAATGGATTGAG+GGG + Chr5:96587150-96587169 None:intergenic 35.0%
!! TGTGTCGTAGATTTTGACAT+GGG + Chr5:96587438-96587457 None:intergenic 35.0%
!!! AGCTTAGTTTTATCGGCATA+AGG + Chr5:96588432-96588451 None:intergenic 35.0%
!!! AGTGAGTTTGATTTTGGAAC+CGG + Chr5:96587486-96587505 None:intergenic 35.0%
AAAAACGGTGAGTTCTGCTT+TGG + Chr5:96588183-96588202 None:intergenic 40.0%
AAAACGGTGAGTTCTGCTTT+GGG + Chr5:96588182-96588201 None:intergenic 40.0%
AACTAAGCTACAAGCTGCAT+TGG - Chr5:96588443-96588462 MsG0580029404.01.T01:CDS 40.0%
AACTCTTACCCTTCTTCATC+CGG - Chr5:96587767-96587786 MsG0580029404.01.T01:intron 40.0%
ACCTCGTGTTGTAAAAGACT+CGG + Chr5:96587048-96587067 None:intergenic 40.0%
ACTTCCACCAACTCAATAAC+AGG + Chr5:96589315-96589334 None:intergenic 40.0%
CAATGAAGTGAGGAAGATGA+AGG + Chr5:96587313-96587332 None:intergenic 40.0%
CGATATAAACGTCGTCGTTT+CGG - Chr5:96588037-96588056 MsG0580029404.01.T01:intron 40.0%
CTGAAGAAAGGACTGTGTAA+AGG - Chr5:96589148-96589167 MsG0580029404.01.T01:intron 40.0%
CTTCTATAATGGTGTTCGGT+TGG + Chr5:96587594-96587613 None:intergenic 40.0%
GATAATGCACGTAGAACTGA+TGG + Chr5:96587464-96587483 None:intergenic 40.0%
GGCAACATAGGCATAAACTA+CGG - Chr5:96587347-96587366 MsG0580029404.01.T01:three_prime_UTR 40.0%
TCAGTTCTACGTGCATTATC+CGG - Chr5:96587464-96587483 MsG0580029404.01.T01:intron 40.0%
TCATTACCATCACCTTTCGA+AGG + Chr5:96588074-96588093 None:intergenic 40.0%
TTCATTGCTCTCACAAATGC+TGG - Chr5:96587326-96587345 MsG0580029404.01.T01:three_prime_UTR 40.0%
TTTGTGAGAGCAATGAAGTG+AGG + Chr5:96587323-96587342 None:intergenic 40.0%
! AGGTATTTCGTCGAGTTGTT+TGG + Chr5:96587649-96587668 None:intergenic 40.0%
! ATTGGATTTTGCTTGTGGTG+AGG - Chr5:96588461-96588480 MsG0580029404.01.T01:CDS 40.0%
! CTCAATCTTTTGCACTCTCA+TGG - Chr5:96587545-96587564 MsG0580029404.01.T01:intron 40.0%
! GAAACAGAGGATTTAGGGTT+TGG + Chr5:96587249-96587268 None:intergenic 40.0%
! GAAAGGTGATGGTAATGAAG+TGG - Chr5:96588076-96588095 MsG0580029404.01.T01:intron 40.0%
! TTGGATTTTGCTTGTGGTGA+GGG - Chr5:96588462-96588481 MsG0580029404.01.T01:CDS 40.0%
!! AAGTTGGTGGAGTATCTTGA+AGG - Chr5:96589178-96589197 MsG0580029404.01.T01:intron 40.0%
!! CTGTGTCGTAGATTTTGACA+TGG + Chr5:96587439-96587458 None:intergenic 40.0%
!! GTTATTGAGTTGGTGGAAGT+GGG - Chr5:96589315-96589334 MsG0580029404.01.T01:intron 40.0%
!! TGAACTGCTTTTGTTGCTGA+GGG + Chr5:96587392-96587411 None:intergenic 40.0%
!! TGCTCTTTTTCGTCAAAACC+CGG - Chr5:96587925-96587944 MsG0580029404.01.T01:intron 40.0%
!! TGGTGTTTCTAAGAGTACCA+CGG - Chr5:96588384-96588403 MsG0580029404.01.T01:intron 40.0%
!! TGTTATTGAGTTGGTGGAAG+TGG - Chr5:96589314-96589333 MsG0580029404.01.T01:intron 40.0%
!! TTGAACTGCTTTTGTTGCTG+AGG + Chr5:96587393-96587412 None:intergenic 40.0%
!! TTGACCTGTTATTGAGTTGG+TGG - Chr5:96589308-96589327 MsG0580029404.01.T01:intron 40.0%
!!! AGAGGATGTGGTTTGTTTTG+TGG + Chr5:96587202-96587221 None:intergenic 40.0%
!!! CACGTGGCATTGTTTTGAAT+CGG + Chr5:96588503-96588522 None:intergenic 40.0%
!!! GTTTGTTTTGTGGAGCAAGT+GGG + Chr5:96587192-96587211 None:intergenic 40.0%
!!! TGGCATTGTTTTGAATCGGA+CGG + Chr5:96588499-96588518 None:intergenic 40.0%
AAACGTCGTCGTTTCGGAAA+CGG - Chr5:96588043-96588062 MsG0580029404.01.T01:intron 45.0%
ACAATGTTCCTTAACCGTGG+AGG - Chr5:96588285-96588304 MsG0580029404.01.T01:intron 45.0%
ACTCTTACCCTTCTTCATCC+GGG - Chr5:96587768-96587787 MsG0580029404.01.T01:intron 45.0%
AGGGTAAGAGTTACCAAGAG+CGG + Chr5:96587759-96587778 None:intergenic 45.0%
CTCACAAATGCTGGCAACAT+AGG - Chr5:96587335-96587354 MsG0580029404.01.T01:three_prime_UTR 45.0%
GCATGTGCCACCAATGTATT+AGG + Chr5:96588532-96588551 None:intergenic 45.0%
TGCTGCAAAGAGTTGTTGGT+TGG + Chr5:96587518-96587537 None:intergenic 45.0%
TTGCTGCTGCAAAGAGTTGT+TGG + Chr5:96587522-96587541 None:intergenic 45.0%
TTTCAAAGCCTCCACGGTTA+AGG + Chr5:96588296-96588315 None:intergenic 45.0%
! ACCGAGTCTTTTACAACACG+AGG - Chr5:96587044-96587063 MsG0580029404.01.T01:three_prime_UTR 45.0%
! GCTGCATTGGATTTTGCTTG+TGG - Chr5:96588456-96588475 MsG0580029404.01.T01:CDS 45.0%
! GTCTTTTACAACACGAGGTG+TGG - Chr5:96587049-96587068 MsG0580029404.01.T01:three_prime_UTR 45.0%
!! AACGGGTTGAATAACTCGGT+CGG + Chr5:96587799-96587818 None:intergenic 45.0%
!! GCTCTTTTTCGTCAAAACCC+GGG - Chr5:96587926-96587945 MsG0580029404.01.T01:intron 45.0%
!! GGTGTTTCTAAGAGTACCAC+GGG - Chr5:96588385-96588404 MsG0580029404.01.T01:intron 45.0%
!! GTTTCTTTCTGAAGTAGGCC+CGG + Chr5:96588234-96588253 None:intergenic 45.0%
!! TTAATGGATTGAGGGGAACG+AGG + Chr5:96587143-96587162 None:intergenic 45.0%
!! TTTCTTTCTGAAGTAGGCCC+GGG + Chr5:96588233-96588252 None:intergenic 45.0%
!!! GCTGGTGGAAGTTGCTATTT+TGG - Chr5:96588592-96588611 MsG0580029404.01.T01:intron 45.0%
!!! GGTGGAAGTTGCTATTTTGG+TGG - Chr5:96588595-96588614 MsG0580029404.01.T01:intron 45.0%
!!! GGTTTGTTTTGTGGAGCAAG+TGG + Chr5:96587193-96587212 None:intergenic 45.0%
!!! GTTTTTTCATGGCGGGTACT+AGG + Chr5:96587669-96587688 None:intergenic 45.0%
AACGTCGTCGTTTCGGAAAC+GGG - Chr5:96588044-96588063 MsG0580029404.01.T01:intron 50.0%
ACAAAGTGGGTCCCACTTAC+AGG - Chr5:96587104-96587123 MsG0580029404.01.T01:three_prime_UTR 50.0%
ACCAAGAGCGGAAAGAGCAA+TGG + Chr5:96587747-96587766 None:intergenic 50.0%
ATAACTCGGTCGGAATGACC+CGG + Chr5:96587789-96587808 None:intergenic 50.0%
CATCAAAAGGGACCCCACTT+AGG - Chr5:96588156-96588175 MsG0580029404.01.T01:intron 50.0%
CCAAGAGCGGAAAGAGCAAT+GGG + Chr5:96587746-96587765 None:intergenic 50.0%
GGAATGACCCGGATGAAGAA+GGG + Chr5:96587778-96587797 None:intergenic 50.0%
GTGTGGTCAGACCTGTAAGT+GGG + Chr5:96587118-96587137 None:intergenic 50.0%
TTACCATGATGACCCAGCAC+CGG - Chr5:96588318-96588337 MsG0580029404.01.T01:intron 50.0%
! AGTTCTGCTTTGGGCCTAAG+TGG + Chr5:96588173-96588192 None:intergenic 50.0%
! GATTTTGCTTGTGGTGAGGG+TGG - Chr5:96588465-96588484 MsG0580029404.01.T01:CDS 50.0%
! GTTCTGCTTTGGGCCTAAGT+GGG + Chr5:96588172-96588191 None:intergenic 50.0%
! TCAGAAAACAGGCACTGTGC+TGG - Chr5:96588574-96588593 MsG0580029404.01.T01:CDS 50.0%
! TTCTGCTTTGGGCCTAAGTG+GGG + Chr5:96588171-96588190 None:intergenic 50.0%
! TTTTCGTCAAAACCCGGGTC+AGG - Chr5:96587931-96587950 MsG0580029404.01.T01:intron 50.0%
!! CGTCGATTTGTGCGTCGAAA+AGG + Chr5:96587986-96588005 None:intergenic 50.0%
!! CTGCTTTTGTTGCTGAGGGT+AGG + Chr5:96587388-96587407 None:intergenic 50.0%
!! TTTTGGTGGTACGTCGTACG+TGG - Chr5:96588609-96588628 MsG0580029404.01.T01:intron 50.0%
CCCATTGCTCTTTCCGCTCT+TGG - Chr5:96587743-96587762 MsG0580029404.01.T01:intron 55.0%
CGGAATGACCCGGATGAAGA+AGG + Chr5:96587779-96587798 None:intergenic 55.0%
GGTGTGGTCAGACCTGTAAG+TGG + Chr5:96587119-96587138 None:intergenic 55.0%
TCGTCGTTTCGGAAACGGGT+TGG - Chr5:96588048-96588067 MsG0580029404.01.T01:intron 55.0%
! ATGGATTGAGGGGAACGAGG+AGG + Chr5:96587140-96587159 None:intergenic 55.0%
! GAAAACAGGCACTGTGCTGG+TGG - Chr5:96588577-96588596 MsG0580029404.01.T01:CDS 55.0%
!! AAGAGTACCACGGGAACACG+TGG - Chr5:96588394-96588413 MsG0580029404.01.T01:intron 55.0%
!! GAAACGGGTTGGCCTTCGAA+AGG - Chr5:96588059-96588078 MsG0580029404.01.T01:intron 55.0%
!! GGTTGGCCTTCGAAAGGTGA+TGG - Chr5:96588065-96588084 MsG0580029404.01.T01:intron 55.0%
AAACCCGGGTCAGGTGGATG+CGG - Chr5:96587940-96587959 MsG0580029404.01.T01:intron 60.0%
AAACCGGTGAAGCCGGTGCT+GGG + Chr5:96588333-96588352 None:intergenic 60.0%
CAACACACCACGTGTTCCCG+TGG + Chr5:96588404-96588423 None:intergenic 60.0%
TCGTCAAAACCCGGGTCAGG+TGG - Chr5:96587934-96587953 MsG0580029404.01.T01:intron 60.0%
TTACCCGCATCCACCTGACC+CGG + Chr5:96587947-96587966 None:intergenic 60.0%
TTGAGGGGAACGAGGAGGTG+TGG + Chr5:96587135-96587154 None:intergenic 60.0%
! AAGCCGGTGCTGGGTCATCA+TGG + Chr5:96588324-96588343 None:intergenic 60.0%
AACCCGGGTCAGGTGGATGC+GGG - Chr5:96587941-96587960 MsG0580029404.01.T01:intron 65.0%
CCACCGGAAACCGGTGAAGC+CGG + Chr5:96588340-96588359 None:intergenic 65.0%
GAAACCGGTGAAGCCGGTGC+TGG + Chr5:96588334-96588353 None:intergenic 65.0%
GGGTCAGGTGGATGCGGGTA+AGG - Chr5:96587946-96587965 MsG0580029404.01.T01:intron 65.0%
TACCCGCATCCACCTGACCC+GGG + Chr5:96587946-96587965 None:intergenic 65.0%
!! GCACCGGCTTCACCGGTTTC+CGG - Chr5:96588334-96588353 MsG0580029404.01.T01:intron 65.0%
!! GCTTCACCGGTTTCCGGTGG+CGG - Chr5:96588340-96588359 MsG0580029404.01.T01:intron 65.0%
!! TGACCCAGCACCGGCTTCAC+CGG - Chr5:96588327-96588346 MsG0580029404.01.T01:intron 65.0%
ACTGCCACTGCCACCGCCAC+CGG + Chr5:96588356-96588375 None:intergenic 70.0%
CTGCCACCGCCACCGGAAAC+CGG + Chr5:96588349-96588368 None:intergenic 70.0%
GGTGGCAGTGGCAGTGGTGG+TGG - Chr5:96588361-96588380 MsG0580029404.01.T01:intron 70.0%
! GTTTCCGGTGGCGGTGGCAG+TGG - Chr5:96588349-96588368 MsG0580029404.01.T01:intron 70.0%
!! CCGGCTTCACCGGTTTCCGG+TGG - Chr5:96588337-96588356 MsG0580029404.01.T01:intron 70.0%
!! GGCAGTGGCAGTGGTGGTGG+TGG - Chr5:96588364-96588383 MsG0580029404.01.T01:intron 70.0%
!! TCACCGGTTTCCGGTGGCGG+TGG - Chr5:96588343-96588362 MsG0580029404.01.T01:intron 70.0%
GGCGGTGGCAGTGGCAGTGG+TGG - Chr5:96588358-96588377 MsG0580029404.01.T01:intron 75.0%
! GGTGGCGGTGGCAGTGGCAG+TGG - Chr5:96588355-96588374 MsG0580029404.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 96587034 96589518 96587034 ID=MsG0580029404.01;Name=MsG0580029404.01
Chr5 mRNA 96587034 96589518 96587034 ID=MsG0580029404.01.T01;Parent=MsG0580029404.01;Name=MsG0580029404.01.T01;_AED=0.40;_eAED=0.44;_QI=0|0|0.33|1|0|0|3|377|105
Chr5 exon 96587034 96587445 96587034 ID=MsG0580029404.01.T01:exon:24533;Parent=MsG0580029404.01.T01
Chr5 exon 96588409 96588599 96588409 ID=MsG0580029404.01.T01:exon:24532;Parent=MsG0580029404.01.T01
Chr5 exon 96589427 96589518 96589427 ID=MsG0580029404.01.T01:exon:24531;Parent=MsG0580029404.01.T01
Chr5 CDS 96589427 96589518 96589427 ID=MsG0580029404.01.T01:cds;Parent=MsG0580029404.01.T01
Chr5 CDS 96588409 96588599 96588409 ID=MsG0580029404.01.T01:cds;Parent=MsG0580029404.01.T01
Chr5 CDS 96587411 96587445 96587411 ID=MsG0580029404.01.T01:cds;Parent=MsG0580029404.01.T01
Chr5 three_prime_UTR 96587034 96587410 96587034 ID=MsG0580029404.01.T01:three_prime_utr;Parent=MsG0580029404.01.T01
Gene Sequence

>MsG0580029404.01.T01

TTGCTCTCAAACCGAGTCTTTTACAACACGAGGTGTGGTCCTATTATTACTCGTCCTTCTGTTTTTTGTTACAAAGTGGGTCCCACTTACAGGTGGATGCGGGTAAGGTTGAGATACTTGAACCTTTTCGACGCACAAATCGACGCTGTTTTCGCTGCACTATCTCGTTTAAAATACGATATAAACGTCGTCGTTTCGGAAACGGGTTGGCCTTCGAAAGGTGATGGTAATGAAGTGGGTGCGAGTGTAGAGAATGCAGCTGCATACAACGCAAATCTCGTCCAGTATGGTAAATGTGAATTTCCAACCGGATACTAG
Protein sequence

>MsG0580029404.01.T01

LLSNRVFYNTRCGPIITRPSVFCYKVGPTYRWMRVRLRYLNLFDAQIDAVFAALSRLKYDINVVVSETGWPSKGDGNEVGASVENAAAYNANLVQYGKCEFPTGY*