Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029482.01.T01 | XP_024638785.1 | 91.489 | 47 | 4 | 0 | 1 | 47 | 464 | 510 | 2.89E-19 | 92.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029482.01.T01 | Q94EJ6 | 78.723 | 47 | 10 | 0 | 1 | 47 | 464 | 510 | 5.71E-18 | 80.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029482.01.T01 | A0A396IC22 | 91.489 | 47 | 4 | 0 | 1 | 47 | 464 | 510 | 1.38e-19 | 92.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580028990.01 | MsG0580029482.01 | 0.806969 | 6.355048e-50 | 3.496747e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029482.01.T01 | MTR_4g098890 | 91.489 | 47 | 4 | 0 | 1 | 47 | 464 | 510 | 3.70e-23 | 92.4 |
| MsG0580029482.01.T01 | MTR_2g008470 | 90.909 | 44 | 4 | 0 | 49 | 92 | 190 | 233 | 6.03e-20 | 83.2 |
| MsG0580029482.01.T01 | MTR_2g008470 | 88.889 | 45 | 5 | 0 | 48 | 92 | 170 | 214 | 6.38e-20 | 83.2 |
| MsG0580029482.01.T01 | MTR_8g055840 | 77.083 | 48 | 10 | 1 | 1 | 47 | 460 | 507 | 3.08e-17 | 75.5 |
| MsG0580029482.01.T01 | MTR_5g040360 | 70.833 | 48 | 13 | 1 | 1 | 47 | 459 | 506 | 2.21e-15 | 70.5 |
| MsG0580029482.01.T01 | MTR_3g040370 | 70.833 | 48 | 13 | 1 | 1 | 47 | 457 | 504 | 2.43e-15 | 70.1 |
| MsG0580029482.01.T01 | MTR_5g061880 | 67.391 | 46 | 15 | 0 | 47 | 92 | 165 | 210 | 3.68e-13 | 63.9 |
| MsG0580029482.01.T01 | MTR_3g073780 | 53.333 | 60 | 23 | 1 | 33 | 92 | 159 | 213 | 9.47e-13 | 62.8 |
| MsG0580029482.01.T01 | MTR_3g073780 | 53.333 | 60 | 23 | 1 | 33 | 92 | 159 | 213 | 1.05e-12 | 62.8 |
| MsG0580029482.01.T01 | MTR_3g073780 | 53.333 | 60 | 23 | 1 | 33 | 92 | 159 | 213 | 1.13e-12 | 62.4 |
| MsG0580029482.01.T01 | MTR_3g071530 | 61.702 | 47 | 18 | 0 | 1 | 47 | 515 | 561 | 4.15e-12 | 60.8 |
| MsG0580029482.01.T01 | MTR_3g068990 | 61.224 | 49 | 17 | 1 | 1 | 47 | 527 | 575 | 9.34e-12 | 60.1 |
| MsG0580029482.01.T01 | MTR_8g076650 | 61.702 | 47 | 18 | 0 | 1 | 47 | 755 | 801 | 1.76e-11 | 59.3 |
| MsG0580029482.01.T01 | MTR_3g096480 | 59.574 | 47 | 19 | 0 | 1 | 47 | 641 | 687 | 2.76e-11 | 58.5 |
| MsG0580029482.01.T01 | MTR_4g011970 | 73.684 | 38 | 10 | 0 | 55 | 92 | 173 | 210 | 3.61e-11 | 58.2 |
| MsG0580029482.01.T01 | MTR_8g432620 | 57.447 | 47 | 20 | 0 | 1 | 47 | 455 | 501 | 7.02e-11 | 57.4 |
| MsG0580029482.01.T01 | MTR_8g071280 | 57.447 | 47 | 20 | 0 | 1 | 47 | 450 | 496 | 7.29e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029482.01.T01 | AT4G18030 | 78.723 | 47 | 10 | 0 | 1 | 47 | 464 | 510 | 5.82e-19 | 80.5 |
| MsG0580029482.01.T01 | AT1G26850 | 79.167 | 48 | 9 | 1 | 1 | 47 | 468 | 515 | 3.29e-18 | 78.6 |
| MsG0580029482.01.T01 | AT1G26850 | 79.167 | 48 | 9 | 1 | 1 | 47 | 468 | 515 | 3.29e-18 | 78.6 |
| MsG0580029482.01.T01 | AT1G22150 | 69.565 | 46 | 14 | 0 | 47 | 92 | 166 | 211 | 1.59e-13 | 65.1 |
| MsG0580029482.01.T01 | AT1G22150 | 69.565 | 46 | 14 | 0 | 47 | 92 | 166 | 211 | 1.65e-13 | 65.1 |
| MsG0580029482.01.T01 | AT1G22150 | 69.565 | 46 | 14 | 0 | 47 | 92 | 166 | 211 | 1.65e-13 | 65.1 |
| MsG0580029482.01.T01 | AT1G22150 | 69.565 | 46 | 14 | 0 | 47 | 92 | 166 | 211 | 1.65e-13 | 65.1 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 28 | 73 | 5.44e-13 | 63.5 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 163 | 208 | 6.12e-13 | 63.5 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 163 | 208 | 6.16e-13 | 63.5 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 163 | 208 | 6.52e-13 | 63.2 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 163 | 208 | 6.52e-13 | 63.2 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 163 | 208 | 6.52e-13 | 63.2 |
| MsG0580029482.01.T01 | AT1G78000 | 65.217 | 46 | 16 | 0 | 47 | 92 | 163 | 208 | 8.38e-13 | 63.2 |
| MsG0580029482.01.T01 | AT3G51895 | 67.442 | 43 | 13 | 1 | 51 | 92 | 203 | 245 | 4.73e-12 | 60.8 |
| MsG0580029482.01.T01 | AT3G56080 | 57.447 | 47 | 20 | 0 | 1 | 47 | 477 | 523 | 5.42e-12 | 60.8 |
| MsG0580029482.01.T01 | AT2G40280 | 59.574 | 47 | 19 | 0 | 1 | 47 | 450 | 496 | 5.45e-12 | 60.8 |
| MsG0580029482.01.T01 | AT3G56080 | 57.447 | 47 | 20 | 0 | 1 | 47 | 477 | 523 | 5.47e-12 | 60.8 |
| MsG0580029482.01.T01 | AT3G51895 | 67.442 | 43 | 13 | 1 | 51 | 92 | 158 | 200 | 5.66e-12 | 60.5 |
| MsG0580029482.01.T01 | AT4G08620 | 69.767 | 43 | 12 | 1 | 51 | 92 | 161 | 203 | 6.94e-12 | 60.5 |
| MsG0580029482.01.T01 | AT2G34300 | 61.702 | 47 | 18 | 0 | 1 | 47 | 622 | 668 | 2.11e-11 | 58.9 |
| MsG0580029482.01.T01 | AT2G34300 | 61.702 | 47 | 18 | 0 | 1 | 47 | 622 | 668 | 2.11e-11 | 58.9 |
| MsG0580029482.01.T01 | AT2G34300 | 61.702 | 47 | 18 | 0 | 1 | 47 | 622 | 668 | 2.11e-11 | 58.9 |
| MsG0580029482.01.T01 | AT5G64030 | 59.574 | 47 | 19 | 0 | 1 | 47 | 681 | 727 | 3.22e-11 | 58.5 |
| MsG0580029482.01.T01 | AT4G19120 | 57.447 | 47 | 20 | 0 | 1 | 47 | 452 | 498 | 3.99e-11 | 58.2 |
| MsG0580029482.01.T01 | AT4G19120 | 57.447 | 47 | 20 | 0 | 1 | 47 | 452 | 498 | 4.06e-11 | 58.2 |
| MsG0580029482.01.T01 | AT4G19120 | 57.447 | 47 | 20 | 0 | 1 | 47 | 452 | 498 | 4.06e-11 | 58.2 |
| MsG0580029482.01.T01 | AT3G15990 | 71.053 | 38 | 11 | 0 | 55 | 92 | 170 | 207 | 5.64e-11 | 57.8 |
| MsG0580029482.01.T01 | AT1G29470 | 59.574 | 47 | 19 | 0 | 1 | 47 | 622 | 668 | 6.86e-11 | 57.8 |
| MsG0580029482.01.T01 | AT1G29470 | 59.574 | 47 | 19 | 0 | 1 | 47 | 622 | 668 | 6.86e-11 | 57.8 |
Find 15 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACTGTCAATTTCTTTGC+AGG | 0.250873 | 5:-97585817 | MsG0580029482.01.T01:CDS |
| AAAGCAAGAGCAAGGTATTC+AGG | 0.293778 | 5:+97585838 | None:intergenic |
| CTATCTCATGCTACCATTGT+TGG | 0.308689 | 5:-97585661 | MsG0580029482.01.T01:CDS |
| ACAATTGCTGACAACACTTT+AGG | 0.337595 | 5:-97586695 | MsG0580029482.01.T01:CDS |
| ATGGACATGAATGCAGGCCT+TGG | 0.423346 | 5:-97586773 | MsG0580029482.01.T01:CDS |
| CAACAATGGTAGCATGAGAT+AGG | 0.448200 | 5:+97585662 | None:intergenic |
| TAAAGTGTTGTCAGCAATTG+TGG | 0.482690 | 5:+97586697 | None:intergenic |
| GACATGAATGCAGGCCTTGG+AGG | 0.485877 | 5:-97586770 | MsG0580029482.01.T01:CDS |
| CTTTGCAGGAGTCACTCAAA+TGG | 0.487638 | 5:-97585803 | MsG0580029482.01.T01:CDS |
| TCATTGGTATTTATCATGAC+TGG | 0.490759 | 5:-97586652 | MsG0580029482.01.T01:intron |
| TATATAATGGACATGAATGC+AGG | 0.539417 | 5:-97586779 | None:intergenic |
| ATCTATGAAAGAGATCTCAT+TGG | 0.556011 | 5:-97586668 | MsG0580029482.01.T01:CDS |
| GGAGTCACTCAAATGGCACT+TGG | 0.578405 | 5:-97585796 | MsG0580029482.01.T01:CDS |
| GGGCAGCTGCAAATCCTCCA+AGG | 0.600004 | 5:+97586756 | None:intergenic |
| TGACAGTAAAAGCAAGAGCA+AGG | 0.617031 | 5:+97585830 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAATCATTTTTTTAGTAT+AGG | + | Chr5:97585851-97585870 | None:intergenic | 10.0% |
| !! | AATTAATTTATTTGATAAGC+AGG | + | Chr5:97586394-97586413 | None:intergenic | 15.0% |
| !!! | ATAGAAAGTTTTTTGATTAT+AGG | - | Chr5:97586036-97586055 | MsG0580029482.01.T01:intron | 15.0% |
| !!! | ATTTTTTTTCTTCATTTTTC+AGG | - | Chr5:97586704-97586723 | MsG0580029482.01.T01:CDS | 15.0% |
| !!! | GATGAATTTTATGTATTAAT+TGG | - | Chr5:97586070-97586089 | MsG0580029482.01.T01:intron | 15.0% |
| !!! | TAACTGATGATTATTTTAAA+TGG | - | Chr5:97585876-97585895 | MsG0580029482.01.T01:intron | 15.0% |
| !!! | TATACAAAATGGTTTTATTT+TGG | - | Chr5:97586413-97586432 | MsG0580029482.01.T01:intron | 15.0% |
| !!! | TTTATGATATAGAAAGTTTT+TGG | - | Chr5:97585945-97585964 | MsG0580029482.01.T01:intron | 15.0% |
| !! | AAGAACCTAATATGATAAAT+TGG | - | Chr5:97586230-97586249 | MsG0580029482.01.T01:intron | 20.0% |
| !! | TGTTTCCAATTTATCATATT+AGG | + | Chr5:97586238-97586257 | None:intergenic | 20.0% |
| ! | TCTGAGAAAATTAGTGAAAT+TGG | + | Chr5:97586155-97586174 | None:intergenic | 25.0% |
| ! | TTATGTATTAATTGGTGAGA+CGG | - | Chr5:97586078-97586097 | MsG0580029482.01.T01:intron | 25.0% |
| ! | TTCAATAACACAAAGTTAGT+TGG | + | Chr5:97586309-97586328 | None:intergenic | 25.0% |
| !! | TCACTAATTTTCTCAGAATT+TGG | - | Chr5:97586158-97586177 | MsG0580029482.01.T01:intron | 25.0% |
| !!! | AAAATGGTTTTATTTTGGCT+TGG | - | Chr5:97586418-97586437 | MsG0580029482.01.T01:intron | 25.0% |
| !!! | AAAGTTTTTGGATCATAGTA+AGG | - | Chr5:97585957-97585976 | MsG0580029482.01.T01:intron | 25.0% |
| !!! | ATTACCTCTTTTTGAAATGA+GGG | - | Chr5:97586115-97586134 | MsG0580029482.01.T01:intron | 25.0% |
| AATTTGGATGCTTAATTACC+AGG | - | Chr5:97586456-97586475 | MsG0580029482.01.T01:intron | 30.0% | |
| ATCTATGAAAGAGATCTCAT+TGG | - | Chr5:97585741-97585760 | MsG0580029482.01.T01:intron | 30.0% | |
| ATTATGCAACTCACCATATT+AGG | + | Chr5:97586334-97586353 | None:intergenic | 30.0% | |
| CAAATATATGTACCTTGTAC+TGG | + | Chr5:97585923-97585942 | None:intergenic | 30.0% | |
| CCTTGAATCACAAAAATCTT+GGG | - | Chr5:97585674-97585693 | MsG0580029482.01.T01:CDS | 30.0% | |
| GGAAAAATGATTGCAAATTG+AGG | - | Chr5:97586477-97586496 | MsG0580029482.01.T01:intron | 30.0% | |
| TCATTGGTATTTATCATGAC+TGG | - | Chr5:97585757-97585776 | MsG0580029482.01.T01:intron | 30.0% | |
| TTTACTGTCAATTTCTTTGC+AGG | - | Chr5:97586592-97586611 | MsG0580029482.01.T01:intron | 30.0% | |
| ! | CCAAGATTTTTGTGATTCAA+GGG | + | Chr5:97585676-97585695 | None:intergenic | 30.0% |
| ! | GTGTTATTGAATGCCTAATA+TGG | - | Chr5:97586318-97586337 | MsG0580029482.01.T01:intron | 30.0% |
| ! | TTTTGAACAATTCCAGTACA+AGG | - | Chr5:97585908-97585927 | MsG0580029482.01.T01:intron | 30.0% |
| !! | CAATTTGCAATCATTTTTCC+TGG | + | Chr5:97586477-97586496 | None:intergenic | 30.0% |
| !!! | GATTACCTCTTTTTGAAATG+AGG | - | Chr5:97586114-97586133 | MsG0580029482.01.T01:intron | 30.0% |
| !!! | GTTTTAAGGTAAACTACCAT+TGG | - | Chr5:97586637-97586656 | MsG0580029482.01.T01:intron | 30.0% |
| !!! | TTTTCTTCATTTTTCAGGCT+TGG | - | Chr5:97586709-97586728 | MsG0580029482.01.T01:CDS | 30.0% |
| AAGGTAAACTACCATTGGAT+TGG | - | Chr5:97586642-97586661 | MsG0580029482.01.T01:intron | 35.0% | |
| AATCACAAAAATCTTGGGTG+AGG | - | Chr5:97585679-97585698 | MsG0580029482.01.T01:CDS | 35.0% | |
| ACAATTGCTGACAACACTTT+AGG | - | Chr5:97585714-97585733 | MsG0580029482.01.T01:intron | 35.0% | |
| CCCTTGAATCACAAAAATCT+TGG | - | Chr5:97585673-97585692 | MsG0580029482.01.T01:CDS | 35.0% | |
| GCTGATTACGATAGAGAATT+TGG | - | Chr5:97586440-97586459 | MsG0580029482.01.T01:intron | 35.0% | |
| ! | AAATGGCACTTGGAGTTTTA+AGG | - | Chr5:97586623-97586642 | MsG0580029482.01.T01:intron | 35.0% |
| ! | ACATTTTGCTAAACTTCGAG+AGG | - | Chr5:97586195-97586214 | MsG0580029482.01.T01:intron | 35.0% |
| ! | CATGCATTTTACCAATCCAA+TGG | + | Chr5:97586656-97586675 | None:intergenic | 35.0% |
| ! | CCCAAGATTTTTGTGATTCA+AGG | + | Chr5:97585677-97585696 | None:intergenic | 35.0% |
| !! | CATTGGATTGGTAAAATGCA+TGG | - | Chr5:97586654-97586673 | MsG0580029482.01.T01:CDS | 35.0% |
| !! | TAAAGTGTTGTCAGCAATTG+TGG | + | Chr5:97585715-97585734 | None:intergenic | 35.0% |
| !! | TATAGGAAGCTGACTTGTAA+GGG | + | Chr5:97585834-97585853 | None:intergenic | 35.0% |
| !!! | ATGAGTCTTATTTTGCAGGT+AGG | - | Chr5:97586528-97586547 | MsG0580029482.01.T01:intron | 35.0% |
| !!! | GTACATGAGTCTTATTTTGC+AGG | - | Chr5:97586524-97586543 | MsG0580029482.01.T01:intron | 35.0% |
| CAACAATGGTAGCATGAGAT+AGG | + | Chr5:97586750-97586769 | None:intergenic | 40.0% | |
| CTAGCCCTCATTTCAAAAAG+AGG | + | Chr5:97586122-97586141 | None:intergenic | 40.0% | |
| CTATCTCATGCTACCATTGT+TGG | - | Chr5:97586748-97586767 | MsG0580029482.01.T01:CDS | 40.0% | |
| TGAAATGAGGGCTAGAGATT+TGG | - | Chr5:97586127-97586146 | MsG0580029482.01.T01:intron | 40.0% | |
| TGACAGTAAAAGCAAGAGCA+AGG | + | Chr5:97586582-97586601 | None:intergenic | 40.0% | |
| ! | AAAGCAAGAGCAAGGTATTC+AGG | + | Chr5:97586574-97586593 | None:intergenic | 40.0% |
| !! | GTATAGGAAGCTGACTTGTA+AGG | + | Chr5:97585835-97585854 | None:intergenic | 40.0% |
| !!! | GATGTGTCGTTTTCCAACAA+TGG | + | Chr5:97586764-97586783 | None:intergenic | 40.0% |
| CTTTGCAGGAGTCACTCAAA+TGG | - | Chr5:97586606-97586625 | MsG0580029482.01.T01:intron | 45.0% | |
| !! | AATAAATTAATTATACAAAA+TGG | - | Chr5:97586402-97586421 | MsG0580029482.01.T01:intron | 5.0% |
| ATGGACATGAATGCAGGCCT+TGG | - | Chr5:97585636-97585655 | MsG0580029482.01.T01:CDS | 50.0% | |
| GGAGTCACTCAAATGGCACT+TGG | - | Chr5:97586613-97586632 | MsG0580029482.01.T01:intron | 50.0% | |
| GACATGAATGCAGGCCTTGG+AGG | - | Chr5:97585639-97585658 | MsG0580029482.01.T01:CDS | 55.0% | |
| GGGCAGCTGCAAATCCTCCA+AGG | + | Chr5:97585656-97585675 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 97585636 | 97586795 | 97585636 | ID=MsG0580029482.01;Name=MsG0580029482.01 |
| Chr5 | mRNA | 97585636 | 97586795 | 97585636 | ID=MsG0580029482.01.T01;Parent=MsG0580029482.01;Name=MsG0580029482.01.T01;_AED=0.56;_eAED=0.56;_QI=0|0|0|0.33|1|1|3|0|99 |
| Chr5 | exon | 97586653 | 97586795 | 97586653 | ID=MsG0580029482.01.T01:exon:25157;Parent=MsG0580029482.01.T01 |
| Chr5 | exon | 97585787 | 97585873 | 97585787 | ID=MsG0580029482.01.T01:exon:25156;Parent=MsG0580029482.01.T01 |
| Chr5 | exon | 97585636 | 97585705 | 97585636 | ID=MsG0580029482.01.T01:exon:25155;Parent=MsG0580029482.01.T01 |
| Chr5 | CDS | 97586653 | 97586795 | 97586653 | ID=MsG0580029482.01.T01:cds;Parent=MsG0580029482.01.T01 |
| Chr5 | CDS | 97585787 | 97585873 | 97585787 | ID=MsG0580029482.01.T01:cds;Parent=MsG0580029482.01.T01 |
| Chr5 | CDS | 97585636 | 97585705 | 97585636 | ID=MsG0580029482.01.T01:cds;Parent=MsG0580029482.01.T01 |
| Gene Sequence |
| Protein sequence |
