Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029655.01.T01 | XP_024639201.1 | 73.333 | 60 | 13 | 1 | 22 | 78 | 181 | 240 | 6.88E-21 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029655.01.T01 | Q9SS37 | 43.103 | 58 | 28 | 1 | 8 | 65 | 175 | 227 | 5.01E-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029655.01.T01 | A0A396HYP0 | 73.333 | 60 | 13 | 1 | 22 | 78 | 183 | 242 | 3.57e-21 | 93.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580029655.01 | MsG0780041120.01 | 0.824586 | 7.447793e-54 | 6.625961e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029655.01.T01 | MTR_5g090340 | 95.349 | 43 | 2 | 0 | 23 | 65 | 229 | 271 | 1.11e-23 | 91.3 |
| MsG0580029655.01.T01 | MTR_5g090340 | 95.349 | 43 | 2 | 0 | 23 | 65 | 232 | 274 | 1.26e-23 | 91.3 |
| MsG0580029655.01.T01 | MTR_3g449880 | 59.649 | 57 | 17 | 1 | 9 | 65 | 175 | 225 | 1.33e-18 | 77.0 |
| MsG0580029655.01.T01 | MTR_5g090440 | 79.070 | 43 | 9 | 0 | 31 | 73 | 11 | 53 | 1.53e-18 | 72.8 |
| MsG0580029655.01.T01 | MTR_3g449880 | 59.649 | 57 | 17 | 1 | 9 | 65 | 153 | 203 | 1.62e-18 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029655.01.T01 | AT3G10260 | 43.103 | 58 | 28 | 1 | 8 | 65 | 175 | 227 | 5.11e-13 | 62.4 |
| MsG0580029655.01.T01 | AT3G10260 | 43.103 | 58 | 28 | 1 | 8 | 65 | 175 | 227 | 5.11e-13 | 62.4 |
| MsG0580029655.01.T01 | AT3G10260 | 43.860 | 57 | 27 | 1 | 9 | 65 | 196 | 247 | 6.89e-13 | 62.4 |
Find 23 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATTCATGTTTATGTAAA+TGG | 0.146269 | 5:-100395356 | MsG0580029655.01.T01:CDS |
| TTTCTCACTTGTGATGAAAT+TGG | 0.188450 | 5:-100395315 | MsG0580029655.01.T01:three_prime_UTR |
| TGCAGGTTGTAGTATGCTTA+TGG | 0.271495 | 5:-100396012 | MsG0580029655.01.T01:CDS |
| CCAGTATAGGAGGTGGATTC+TGG | 0.278199 | 5:-100395415 | MsG0580029655.01.T01:CDS |
| CTATCCAAGCTTCTTTCCTT+TGG | 0.317711 | 5:+100395376 | None:intergenic |
| TATCCAAGCTTCTTTCCTTT+GGG | 0.342722 | 5:+100395377 | None:intergenic |
| CCAGAATCCACCTCCTATAC+TGG | 0.380334 | 5:+100395415 | None:intergenic |
| CTGGTGCTGTGATTGGGAAC+TGG | 0.386885 | 5:-100395988 | MsG0580029655.01.T01:CDS |
| TTATGGGCTGGTGCTGTGAT+TGG | 0.407728 | 5:-100395995 | MsG0580029655.01.T01:CDS |
| TCATACCTTTCGTAGAGAAC+CGG | 0.437296 | 5:+100395486 | None:intergenic |
| GCAGGTTGTAGTATGCTTAT+GGG | 0.447620 | 5:-100396011 | MsG0580029655.01.T01:CDS |
| TATGGGCTGGTGCTGTGATT+GGG | 0.451379 | 5:-100395994 | MsG0580029655.01.T01:CDS |
| ATCAGATGCAAAACCAGTAT+AGG | 0.521173 | 5:-100395428 | MsG0580029655.01.T01:CDS |
| AATCCCAAAGGAAAGAAGCT+TGG | 0.522570 | 5:-100395380 | MsG0580029655.01.T01:CDS |
| TGTTGCTGCACATACACTCC+CGG | 0.528499 | 5:-100395505 | MsG0580029655.01.T01:CDS |
| GATTGATAACTTCGTGTACA+AGG | 0.536766 | 5:-100395457 | MsG0580029655.01.T01:CDS |
| ATCATGCTTAAAACATCTGC+AGG | 0.552931 | 5:-100396029 | MsG0580029655.01.T01:CDS |
| TGCAAAACCAGTATAGGAGG+TGG | 0.557512 | 5:-100395422 | MsG0580029655.01.T01:CDS |
| GTTGTAGTATGCTTATGGGC+TGG | 0.564353 | 5:-100396007 | MsG0580029655.01.T01:CDS |
| CATACCTTTCGTAGAGAACC+GGG | 0.565792 | 5:+100395487 | None:intergenic |
| CACTCCCGGTTCTCTACGAA+AGG | 0.578629 | 5:-100395491 | MsG0580029655.01.T01:CDS |
| AGATGCAAAACCAGTATAGG+AGG | 0.599832 | 5:-100395425 | MsG0580029655.01.T01:CDS |
| TTTGCTTAGCAAAATCCCAA+AGG | 0.677295 | 5:-100395392 | MsG0580029655.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTAGAGATAATATTTTT+AGG | + | Chr5:100395447-100395466 | None:intergenic | 10.0% |
| !!! | TATAATTTCTTTTTCTATGT+TGG | - | Chr5:100396020-100396039 | MsG0580029655.01.T01:CDS | 15.0% |
| !! | AATAATTAGAGAAAACATCA+TGG | + | Chr5:100395381-100395400 | None:intergenic | 20.0% |
| !! | TAGATTCATGTTTATGTAAA+TGG | - | Chr5:100395920-100395939 | MsG0580029655.01.T01:intron | 20.0% |
| !!! | GTTAAAACTTTGTTATGTTA+CGG | - | Chr5:100395689-100395708 | MsG0580029655.01.T01:intron | 20.0% |
| !!! | GTTTTCTCTAATTATTAAGT+TGG | - | Chr5:100395385-100395404 | MsG0580029655.01.T01:CDS | 20.0% |
| ! | AACACATGTATCAACAAAAA+AGG | + | Chr5:100395356-100395375 | None:intergenic | 25.0% |
| ! | CTGTTGAAATAGTATTTCAT+CGG | - | Chr5:100395482-100395501 | MsG0580029655.01.T01:CDS | 25.0% |
| !!! | CTTTGTTTTGTTATGTCTTA+AGG | - | Chr5:100395745-100395764 | MsG0580029655.01.T01:intron | 25.0% |
| !!! | TTTTTTACTGTCGTGTATAT+CGG | - | Chr5:100395316-100395335 | MsG0580029655.01.T01:three_prime_UTR | 25.0% |
| !!! | TTTTTTGTTGATACATGTGT+TGG | - | Chr5:100395355-100395374 | MsG0580029655.01.T01:CDS | 25.0% |
| ACATGTATCAACAAAAAAGG+AGG | + | Chr5:100395353-100395372 | None:intergenic | 30.0% | |
| TCAAGAATTCTCTCTTTGTT+TGG | + | Chr5:100395528-100395547 | None:intergenic | 30.0% | |
| TTTCTCACTTGTGATGAAAT+TGG | - | Chr5:100395961-100395980 | MsG0580029655.01.T01:CDS | 30.0% | |
| ! | CAAGAATTCTCTCTTTGTTT+GGG | + | Chr5:100395527-100395546 | None:intergenic | 30.0% |
| !!! | TGTGTTTGTTGCTTTTACTT+TGG | - | Chr5:100395991-100396010 | MsG0580029655.01.T01:CDS | 30.0% |
| AACAGCAAACTCGGTTAATT+GGG | + | Chr5:100395594-100395613 | None:intergenic | 35.0% | |
| ATCAGATGCAAAACCAGTAT+AGG | - | Chr5:100395848-100395867 | MsG0580029655.01.T01:intron | 35.0% | |
| TAACAGCAAACTCGGTTAAT+TGG | + | Chr5:100395595-100395614 | None:intergenic | 35.0% | |
| TATCCAAGCTTCTTTCCTTT+GGG | + | Chr5:100395902-100395921 | None:intergenic | 35.0% | |
| TTACCACGAAACACTTAACA+AGG | + | Chr5:100395657-100395676 | None:intergenic | 35.0% | |
| TTTGCTTAGCAAAATCCCAA+AGG | - | Chr5:100395884-100395903 | MsG0580029655.01.T01:intron | 35.0% | |
| ! | GATTGATAACTTCGTGTACA+AGG | - | Chr5:100395819-100395838 | MsG0580029655.01.T01:intron | 35.0% |
| AATCCCAAAGGAAAGAAGCT+TGG | - | Chr5:100395896-100395915 | MsG0580029655.01.T01:intron | 40.0% | |
| AGATGCAAAACCAGTATAGG+AGG | - | Chr5:100395851-100395870 | MsG0580029655.01.T01:intron | 40.0% | |
| CTATCCAAGCTTCTTTCCTT+TGG | + | Chr5:100395903-100395922 | None:intergenic | 40.0% | |
| GCAGGTTGTAGTATGCTTAT+GGG | - | Chr5:100395265-100395284 | MsG0580029655.01.T01:three_prime_UTR | 40.0% | |
| TCACCTTGTTAAGTGTTTCG+TGG | - | Chr5:100395651-100395670 | MsG0580029655.01.T01:intron | 40.0% | |
| TCATACCTTTCGTAGAGAAC+CGG | + | Chr5:100395793-100395812 | None:intergenic | 40.0% | |
| TGCAGGTTGTAGTATGCTTA+TGG | - | Chr5:100395264-100395283 | MsG0580029655.01.T01:three_prime_UTR | 40.0% | |
| !! | AACCGAGTTTGCTGTTATCT+CGG | - | Chr5:100395598-100395617 | MsG0580029655.01.T01:intron | 40.0% |
| CACCGAGATAACAGCAAACT+CGG | + | Chr5:100395603-100395622 | None:intergenic | 45.0% | |
| CATACCTTTCGTAGAGAACC+GGG | + | Chr5:100395792-100395811 | None:intergenic | 45.0% | |
| GTTGTAGTATGCTTATGGGC+TGG | - | Chr5:100395269-100395288 | MsG0580029655.01.T01:three_prime_UTR | 45.0% | |
| TGCAAAACCAGTATAGGAGG+TGG | - | Chr5:100395854-100395873 | MsG0580029655.01.T01:intron | 45.0% | |
| !! | TTTGCTGTTATCTCGGTGTG+TGG | - | Chr5:100395605-100395624 | MsG0580029655.01.T01:intron | 45.0% |
| CCAGAATCCACCTCCTATAC+TGG | + | Chr5:100395864-100395883 | None:intergenic | 50.0% | |
| TGTTGCTGCACATACACTCC+CGG | - | Chr5:100395771-100395790 | MsG0580029655.01.T01:intron | 50.0% | |
| ! | CCAGTATAGGAGGTGGATTC+TGG | - | Chr5:100395861-100395880 | MsG0580029655.01.T01:intron | 50.0% |
| !! | TATGGGCTGGTGCTGTGATT+GGG | - | Chr5:100395282-100395301 | MsG0580029655.01.T01:three_prime_UTR | 50.0% |
| !! | TTATGGGCTGGTGCTGTGAT+TGG | - | Chr5:100395281-100395300 | MsG0580029655.01.T01:three_prime_UTR | 50.0% |
| CACTCCCGGTTCTCTACGAA+AGG | - | Chr5:100395785-100395804 | MsG0580029655.01.T01:intron | 55.0% | |
| !! | CTGGTGCTGTGATTGGGAAC+TGG | - | Chr5:100395288-100395307 | MsG0580029655.01.T01:three_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 100395250 | 100396048 | 100395250 | ID=MsG0580029655.01;Name=MsG0580029655.01 |
| Chr5 | mRNA | 100395250 | 100396048 | 100395250 | ID=MsG0580029655.01.T01;Parent=MsG0580029655.01;Name=MsG0580029655.01.T01;_AED=0.38;_eAED=0.38;_QI=0|1|0.5|1|0|0|2|94|91 |
| Chr5 | exon | 100395250 | 100395531 | 100395250 | ID=MsG0580029655.01.T01:exon:26436;Parent=MsG0580029655.01.T01 |
| Chr5 | exon | 100395961 | 100396048 | 100395961 | ID=MsG0580029655.01.T01:exon:26435;Parent=MsG0580029655.01.T01 |
| Chr5 | CDS | 100395961 | 100396048 | 100395961 | ID=MsG0580029655.01.T01:cds;Parent=MsG0580029655.01.T01 |
| Chr5 | CDS | 100395344 | 100395531 | 100395344 | ID=MsG0580029655.01.T01:cds;Parent=MsG0580029655.01.T01 |
| Chr5 | three_prime_UTR | 100395250 | 100395343 | 100395250 | ID=MsG0580029655.01.T01:three_prime_utr;Parent=MsG0580029655.01.T01 |
| Gene Sequence |
| Protein sequence |
