AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029735.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029735.01.T01 MTR_5g091650 74.678 233 8 2 1 230 1 185 2.78e-101 294
MsG0580029735.01.T01 MTR_5g091650 74.678 233 8 2 1 230 1 185 1.68e-100 295
MsG0580029735.01.T01 MTR_3g048280 67.052 173 48 4 68 232 20 191 3.22e-72 221
MsG0580029735.01.T01 MTR_7g111600 63.750 80 29 0 153 232 160 239 7.96e-29 110
MsG0580029735.01.T01 MTR_2g049670 63.750 80 28 1 153 232 158 236 1.38e-28 111
MsG0580029735.01.T01 MTR_7g111600 63.750 80 29 0 153 232 160 239 1.79e-28 110
MsG0580029735.01.T01 MTR_7g111600 63.750 80 29 0 153 232 160 239 2.15e-28 110
MsG0580029735.01.T01 MTR_1g084820 56.818 88 37 1 143 230 410 496 3.51e-27 110
MsG0580029735.01.T01 MTR_1g084820 56.818 88 37 1 143 230 410 496 6.03e-27 110
MsG0580029735.01.T01 MTR_8g088120 61.429 70 27 0 161 230 56 125 1.70e-24 98.2
MsG0580029735.01.T01 MTR_8g089400 49.038 104 51 2 129 230 32 135 2.87e-24 96.7
MsG0580029735.01.T01 MTR_8g089400 49.038 104 51 2 129 230 12 115 2.98e-24 95.9
MsG0580029735.01.T01 MTR_1g056640 50.617 81 39 1 150 229 403 483 2.40e-21 93.6
MsG0580029735.01.T01 MTR_4g079530 53.165 79 36 1 153 230 37 115 3.94e-21 86.7
MsG0580029735.01.T01 MTR_4g079530 46.392 97 48 2 153 245 37 133 5.07e-21 87.8
MsG0580029735.01.T01 MTR_8g085420 52.500 80 36 2 152 230 31 109 8.51e-21 86.3
MsG0580029735.01.T01 MTR_8g085440 52.500 80 36 2 152 230 31 109 1.49e-20 84.7
MsG0580029735.01.T01 MTR_5g015720 52.500 80 36 2 153 230 329 408 1.87e-20 90.5
MsG0580029735.01.T01 MTR_4g132280 50.000 82 40 1 152 232 381 462 2.24e-20 90.9
MsG0580029735.01.T01 MTR_8g085390 54.545 88 37 3 145 230 21 107 3.85e-20 85.9
MsG0580029735.01.T01 MTR_8g102340 48.148 81 41 1 151 230 332 412 7.13e-20 89.0
MsG0580029735.01.T01 MTR_3g082080 47.500 80 40 2 153 231 157 235 1.16e-19 85.9
MsG0580029735.01.T01 MTR_1g022160 47.959 98 43 4 152 246 390 482 3.40e-19 87.0
MsG0580029735.01.T01 MTR_5g085580 48.148 81 41 1 150 229 379 459 4.75e-19 86.7
MsG0580029735.01.T01 MTR_6g088885 45.783 83 43 2 150 230 366 448 4.84e-19 86.7
MsG0580029735.01.T01 MTR_8g085400 51.899 79 35 2 152 230 32 107 1.32e-18 79.7
MsG0580029735.01.T01 MTR_5g097420 49.367 79 39 1 153 230 30 108 2.89e-18 79.0
MsG0580029735.01.T01 MTR_1g022160 48.780 82 36 3 152 230 390 468 3.66e-18 84.3
MsG0580029735.01.T01 MTR_1g022160 48.148 81 36 3 152 229 390 467 5.69e-18 83.6
MsG0580029735.01.T01 MTR_3g083580 41.905 105 60 1 152 255 361 465 1.02e-17 82.8
MsG0580029735.01.T01 MTR_8g085720 50.633 79 38 1 153 230 364 442 5.67e-17 80.9
MsG0580029735.01.T01 MTR_4g069940 46.914 81 42 1 151 230 373 453 6.33e-17 80.5
MsG0580029735.01.T01 MTR_1g116870 46.341 82 42 2 150 230 273 353 1.70e-16 78.6
MsG0580029735.01.T01 MTR_4g083500 40.566 106 55 3 132 230 375 479 1.94e-16 79.3
MsG0580029735.01.T01 MTR_1g031900 42.308 104 57 2 132 232 338 441 2.27e-16 79.0
MsG0580029735.01.T01 MTR_1g031900 42.308 104 57 2 132 232 338 441 5.26e-16 77.8
MsG0580029735.01.T01 MTR_4g133735 45.000 80 43 1 152 230 27 106 2.51e-15 70.9
MsG0580029735.01.T01 MTR_3g065460 40.909 88 51 1 146 232 354 441 2.81e-15 75.9
MsG0580029735.01.T01 MTR_5g078200 39.773 88 52 1 146 232 353 440 5.16e-15 75.1
MsG0580029735.01.T01 MTR_5g078200 39.773 88 52 1 146 232 320 407 5.81e-15 75.1
MsG0580029735.01.T01 MTR_7g026340 45.679 81 42 2 151 230 363 442 7.10e-15 74.3
MsG0580029735.01.T01 MTR_4g133730 41.463 82 47 1 152 232 27 108 8.86e-15 69.3
MsG0580029735.01.T01 MTR_8g012400 37.209 86 53 1 146 230 367 452 4.88e-14 72.0
MsG0580029735.01.T01 MTR_8g012400 37.209 86 53 1 146 230 287 372 6.42e-14 71.2
MsG0580029735.01.T01 MTR_4g088155 36.697 109 65 2 134 238 354 462 2.40e-13 70.1
MsG0580029735.01.T01 MTR_4g083070 45.679 81 42 2 153 232 21 100 5.71e-13 66.2
MsG0580029735.01.T01 MTR_4g109490 43.210 81 43 3 151 230 22 100 9.29e-13 66.2
MsG0580029735.01.T01 MTR_7g053500 35.644 101 63 2 132 230 275 375 1.27e-12 67.4
MsG0580029735.01.T01 MTR_7g053500 36.667 90 56 1 142 230 363 452 1.30e-12 67.8
MsG0580029735.01.T01 MTR_3g116510 39.583 96 52 3 138 230 222 314 1.73e-12 67.0
MsG0580029735.01.T01 MTR_3g116510 39.583 96 52 3 138 230 356 448 1.88e-12 67.4
MsG0580029735.01.T01 MTR_2g072500 38.750 80 46 2 152 230 7 84 4.52e-12 61.2
MsG0580029735.01.T01 MTR_5g044530 39.286 84 49 2 150 232 19 101 4.54e-12 65.1
MsG0580029735.01.T01 MTR_1g053320 33.884 121 67 6 153 268 374 486 3.69e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029735.01.T01 AT2G30933 55.340 103 43 2 131 230 57 159 6.69e-34 121
MsG0580029735.01.T01 AT2G30933 55.340 103 43 2 131 230 57 159 6.69e-34 121
MsG0580029735.01.T01 AT2G30933 55.340 103 43 2 131 230 57 159 4.24e-33 121
MsG0580029735.01.T01 AT1G29380 64.557 79 28 0 153 231 148 226 1.72e-30 114
MsG0580029735.01.T01 AT1G29380 64.557 79 28 0 153 231 148 226 1.72e-30 114
MsG0580029735.01.T01 AT1G29380 63.750 80 29 0 153 232 148 227 3.01e-30 114
MsG0580029735.01.T01 AT1G29380 63.750 80 29 0 153 232 148 227 5.73e-30 115
MsG0580029735.01.T01 AT1G09460 60.241 83 31 1 150 232 134 214 2.60e-27 108
MsG0580029735.01.T01 AT5G55180 51.087 92 44 1 147 237 366 457 1.02e-24 102
MsG0580029735.01.T01 AT4G05430 48.544 103 50 2 130 231 27 127 5.37e-24 94.4
MsG0580029735.01.T01 AT4G05430 48.039 102 50 2 130 230 2 101 1.98e-23 93.2
MsG0580029735.01.T01 AT4G05430 48.039 102 50 2 130 230 27 126 2.56e-23 94.0
MsG0580029735.01.T01 AT5G55180 53.571 84 38 1 147 229 366 449 6.03e-23 97.8
MsG0580029735.01.T01 AT2G05790 41.818 110 63 1 121 229 352 461 8.32e-21 92.0
MsG0580029735.01.T01 AT5G67460 50.000 80 39 1 153 231 293 372 1.24e-20 90.5
MsG0580029735.01.T01 AT4G34480 50.617 81 38 2 151 230 251 330 2.32e-20 90.1
MsG0580029735.01.T01 AT4G26830 50.617 81 39 1 150 229 364 444 3.32e-20 90.1
MsG0580029735.01.T01 AT4G26830 50.617 81 39 1 150 229 368 448 3.64e-20 90.1
MsG0580029735.01.T01 AT4G34480 50.617 81 38 2 151 230 362 441 4.06e-20 90.1
MsG0580029735.01.T01 AT3G58100 46.914 81 42 1 153 232 41 121 5.05e-20 85.5
MsG0580029735.01.T01 AT5G67460 49.367 79 39 1 153 230 293 371 6.47e-20 88.6
MsG0580029735.01.T01 AT3G58100 46.914 81 42 1 153 232 117 197 7.53e-20 86.7
MsG0580029735.01.T01 AT1G11820 50.617 81 39 1 152 231 380 460 8.70e-20 89.0
MsG0580029735.01.T01 AT4G13600 50.633 79 37 2 153 230 61 138 3.10e-19 84.3
MsG0580029735.01.T01 AT1G11820 48.780 82 41 1 152 232 380 461 4.87e-19 87.0
MsG0580029735.01.T01 AT2G04910 42.857 98 55 1 151 247 13 110 5.64e-18 78.2
MsG0580029735.01.T01 AT5G63250 39.640 111 61 3 130 237 22 129 1.43e-17 77.4
MsG0580029735.01.T01 AT4G16165 43.902 82 45 1 152 232 25 106 1.49e-17 76.6
MsG0580029735.01.T01 AT5G63230 45.000 80 42 2 153 231 26 104 2.07e-17 76.3
MsG0580029735.01.T01 AT4G29360 46.835 79 41 1 153 230 401 479 2.67e-17 81.6
MsG0580029735.01.T01 AT1G66250 42.718 103 56 2 133 232 346 448 2.83e-17 81.6
MsG0580029735.01.T01 AT4G29360 46.835 79 41 1 153 230 391 469 2.95e-17 81.6
MsG0580029735.01.T01 AT2G42930 43.590 78 43 1 153 230 40 116 4.00e-17 76.3
MsG0580029735.01.T01 AT5G35740 49.383 81 37 3 153 230 30 109 4.10e-17 75.9
MsG0580029735.01.T01 AT5G56590 44.304 79 43 1 153 230 369 447 4.45e-17 81.3
MsG0580029735.01.T01 AT1G66870 40.000 80 47 1 152 230 52 131 5.04e-17 76.3
MsG0580029735.01.T01 AT2G01630 43.902 82 45 1 152 232 359 440 6.94e-17 80.5
MsG0580029735.01.T01 AT2G01630 45.000 80 43 1 152 230 359 438 7.63e-17 80.1
MsG0580029735.01.T01 AT2G04910 46.914 81 42 1 151 230 13 93 8.17e-17 74.7
MsG0580029735.01.T01 AT1G66855 41.463 82 47 1 150 230 23 104 9.02e-17 74.7
MsG0580029735.01.T01 AT1G66870 40.000 80 47 1 152 230 25 104 1.16e-16 74.3
MsG0580029735.01.T01 AT2G16230 47.500 80 40 2 152 230 365 443 1.48e-16 79.7
MsG0580029735.01.T01 AT1G66852 39.241 79 48 0 152 230 25 103 2.94e-16 73.2
MsG0580029735.01.T01 AT5G63225 43.210 81 44 2 153 232 25 104 2.97e-16 73.2
MsG0580029735.01.T01 AT3G13560 45.679 81 43 1 151 230 360 440 1.71e-15 76.3
MsG0580029735.01.T01 AT3G13560 45.679 81 43 1 151 230 360 440 1.71e-15 76.3
MsG0580029735.01.T01 AT3G13560 45.679 81 43 1 151 230 360 440 1.71e-15 76.3
MsG0580029735.01.T01 AT3G13560 45.679 81 43 1 151 230 360 440 1.71e-15 76.3
MsG0580029735.01.T01 AT3G13560 45.679 81 43 1 151 230 360 440 1.71e-15 76.3
MsG0580029735.01.T01 AT1G79480 43.038 79 43 2 153 230 310 387 3.06e-15 75.5
MsG0580029735.01.T01 AT1G79480 43.038 79 43 2 153 230 311 388 3.13e-15 75.5
MsG0580029735.01.T01 AT3G55430 41.667 84 47 2 150 232 363 445 6.66e-15 74.7
MsG0580029735.01.T01 AT5G61130 44.318 88 44 3 151 234 19 105 9.32e-15 71.6
MsG0580029735.01.T01 AT2G39640 43.038 79 43 2 153 230 359 436 1.50e-14 73.6
MsG0580029735.01.T01 AT2G39640 35.802 81 50 2 151 230 458 537 2.22e-12 67.4
MsG0580029735.01.T01 AT5G63240 37.500 88 52 2 153 237 40 127 1.58e-14 69.3
MsG0580029735.01.T01 AT1G18650 40.741 81 46 2 151 230 19 98 3.14e-14 68.6
MsG0580029735.01.T01 AT1G18650 40.741 81 46 2 151 230 19 98 4.95e-14 69.3
MsG0580029735.01.T01 AT4G09462 40.000 80 46 2 152 230 28 106 1.57e-13 65.9
MsG0580029735.01.T01 AT5G53600 40.000 80 45 2 152 230 28 105 1.79e-13 65.9
MsG0580029735.01.T01 AT5G24318 34.058 138 67 6 98 230 192 310 3.70e-13 68.9
MsG0580029735.01.T01 AT3G23770 41.667 84 48 1 148 230 382 465 4.20e-13 69.3
MsG0580029735.01.T01 AT5G24318 34.058 138 67 6 98 230 331 449 4.36e-13 69.3
MsG0580029735.01.T01 AT5G24318 34.058 138 67 6 98 230 331 449 4.70e-13 68.9
MsG0580029735.01.T01 AT4G09465 38.750 80 47 2 152 230 30 108 6.25e-13 64.3
MsG0580029735.01.T01 AT4G09467 38.750 80 47 2 152 230 30 108 6.45e-13 64.3
MsG0580029735.01.T01 AT4G09464 38.750 80 47 2 152 230 30 108 6.45e-13 64.3
MsG0580029735.01.T01 AT4G09466 38.750 80 47 2 152 230 30 108 2.33e-12 62.8
MsG0580029735.01.T01 AT5G08000 45.652 92 45 3 147 234 34 124 2.47e-12 65.1
MsG0580029735.01.T01 AT1G69295 39.080 87 50 3 147 232 15 99 3.04e-12 65.1
MsG0580029735.01.T01 AT1G78520 41.250 80 44 2 152 230 31 108 3.28e-12 62.4
MsG0580029735.01.T01 AT1G69295 40.964 83 46 3 151 232 15 95 3.90e-12 64.7
MsG0580029735.01.T01 AT5G53610 40.506 79 44 2 153 230 28 104 6.94e-12 61.2
MsG0580029735.01.T01 AT4G09090 40.506 79 45 2 153 230 31 108 9.59e-12 61.2
MsG0580029735.01.T01 AT4G14080 38.298 94 57 1 138 230 374 467 2.37e-11 63.9
MsG0580029735.01.T01 AT2G43670 41.975 81 44 2 151 230 33 111 4.10e-11 59.7
MsG0580029735.01.T01 AT1G26450 39.241 79 45 3 147 224 15 91 4.76e-11 61.2
MsG0580029735.01.T01 AT5G08000 45.455 88 43 3 151 234 19 105 7.81e-11 60.5

Find 47 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCAAATAGCTGCAATTT+TGG 0.156361 5:+101355885 MsG0580029735.01.T01:CDS
CCACCAAAATTGCAGCTATT+TGG 0.187614 5:-101355888 None:intergenic
GCAAGATCATGAATAGAATT+TGG 0.189393 5:-101355825 None:intergenic
CTTATGATAATACTTATTAA+AGG 0.218122 5:-101355856 None:intergenic
TGCTTTGACTGTTACATATA+GGG 0.244232 5:+101354455 MsG0580029735.01.T01:CDS
TCAATTATGATTTCAAATTC+AGG 0.287653 5:+101355435 MsG0580029735.01.T01:CDS
CTGCTTTGACTGTTACATAT+AGG 0.299560 5:+101354454 MsG0580029735.01.T01:CDS
TTGGAGTGGTAATATCCTTT+TGG 0.304034 5:-101355554 None:intergenic
TTAGACTATGCTTGTGGATA+TGG 0.306358 5:+101355756 MsG0580029735.01.T01:CDS
ACTGTGTCTGGATTGTAATT+AGG 0.347083 5:-101355621 None:intergenic
AGTCCAAGTGCGTCGCAAAT+TGG 0.359009 5:+101355720 MsG0580029735.01.T01:CDS
ATTGTTGGAATGGTAGTAAT+TGG 0.380518 5:-101355573 None:intergenic
ATGATAGGAAAAGCCTTACT+TGG 0.385179 5:-101355936 None:intergenic
TGCAATTAATATTATTGTAA+AGG 0.385670 5:-101355973 None:intergenic
ACTGTGGTTGGATTGGTATA+TGG 0.399145 5:-101355663 None:intergenic
TTTGAATTTGATATAACTAA+AGG 0.415363 5:-101355997 None:intergenic
GTTGCAATGAAAATTATGAT+AGG 0.438492 5:-101355951 None:intergenic
AAATTGCAGCTATTTGGAAC+AGG 0.446661 5:-101355882 None:intergenic
GGATCAGGTGTTGTAATTGT+TGG 0.447684 5:-101355588 None:intergenic
AGAATTCACTGTGGTTGGAT+TGG 0.457116 5:-101355670 None:intergenic
CAATAAAATCAAAGATGAAA+AGG 0.460561 5:+101353781 None:intergenic
GAAACTGCATGTGGAAGTAC+TGG 0.461877 5:-101354409 None:intergenic
CACTAGCACTAATCCAAGTA+AGG 0.469500 5:+101355923 MsG0580029735.01.T01:CDS
GGAAAAGAATTCACTGTGGT+TGG 0.470603 5:-101355675 None:intergenic
CAGTTTCTTGAAAACATACA+AGG 0.471693 5:+101354425 MsG0580029735.01.T01:CDS
AAACCAATTTGCGACGCACT+TGG 0.472200 5:-101355723 None:intergenic
GCAACACACCAACTTGAACC+TGG 0.494893 5:-101355696 None:intergenic
AGCAAAGGATTTGAGTTAGT+AGG 0.502237 5:-101353830 None:intergenic
TTTCAAATTCAGGTTCAAGT+AGG 0.505311 5:+101355445 MsG0580029735.01.T01:CDS
AGGTTCAAGTAGGACAGAAA+AGG 0.511879 5:+101355455 MsG0580029735.01.T01:CDS
GGAAGTACTGGAGAAAAGGT+TGG 0.517972 5:-101354397 None:intergenic
GAAGTTGGAGAAACTGTGTC+TGG 0.524569 5:-101355633 None:intergenic
GTATATGGAAGTGTTGAAGT+TGG 0.524882 5:-101355648 None:intergenic
CTAACTCAAATCCTTTGCTC+AGG 0.540740 5:+101353834 MsG0580029735.01.T01:CDS
TATGTAACAGTCAAAGCAGT+TGG 0.542439 5:-101354450 None:intergenic
AGGTGTTGTAATTGTTGGAA+TGG 0.560140 5:-101355583 None:intergenic
GCTTTGACTGTTACATATAG+GGG 0.563106 5:+101354456 MsG0580029735.01.T01:CDS
GGATTGTAACAGCTTCCACC+AGG 0.569678 5:-101355804 None:intergenic
ATGTGGAAGTACTGGAGAAA+AGG 0.575987 5:-101354401 None:intergenic
GTTGCATTAGACTATGCTTG+TGG 0.587339 5:+101355750 MsG0580029735.01.T01:CDS
GATTGTTCAGCAATTCAACC+TGG 0.623093 5:+101355786 MsG0580029735.01.T01:CDS
TGTTCAGCAATTCAACCTGG+TGG 0.630105 5:+101355789 MsG0580029735.01.T01:CDS
GACTATGCTTGTGGATATGG+TGG 0.650066 5:+101355759 MsG0580029735.01.T01:CDS
AGTTTCTTGAAAACATACAA+GGG 0.693701 5:+101354426 MsG0580029735.01.T01:CDS
TGGAATGGTAGTAATTGGAG+TGG 0.718590 5:-101355568 None:intergenic
ATATAGGGGTCTCAGTACAG+AGG 0.732537 5:+101354470 MsG0580029735.01.T01:CDS
ACCTGGAAAAGAATTCACTG+TGG 0.818426 5:-101355679 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAAGATATAAATTTA+TGG - Chr5:101354228-101354247 None:intergenic 10.0%
!! AACTAATTAGTATTATTATA+TGG - Chr5:101354781-101354800 None:intergenic 10.0%
!! ACTAATTAGTATTATTATAT+GGG - Chr5:101354780-101354799 None:intergenic 10.0%
!!! AAATTTATATCTTCTTTTTA+TGG + Chr5:101354229-101354248 MsG0580029735.01.T01:intron 10.0%
!! CTTATGATAATACTTATTAA+AGG - Chr5:101355859-101355878 None:intergenic 15.0%
!! TGCAATTAATATTATTGTAA+AGG - Chr5:101355976-101355995 None:intergenic 15.0%
!! TTTGAATTTGATATAACTAA+AGG - Chr5:101356000-101356019 None:intergenic 15.0%
!! AACATTTCCCTTATTATATT+AGG - Chr5:101354586-101354605 None:intergenic 20.0%
!! ACATGGTTATATATTTATGT+TGG + Chr5:101354149-101354168 MsG0580029735.01.T01:intron 20.0%
!! GTTAATGTAGTTTCAATATT+TGG - Chr5:101355229-101355248 None:intergenic 20.0%
!! TATATTTATGTTGGACTAAT+TGG + Chr5:101354158-101354177 MsG0580029735.01.T01:intron 20.0%
!! TCAATTATGATTTCAAATTC+AGG + Chr5:101355435-101355454 MsG0580029735.01.T01:CDS 20.0%
!! TGTCATAATATTCATTAACT+AGG + Chr5:101355074-101355093 MsG0580029735.01.T01:intron 20.0%
!! TTGTAATTAGGATTGAAAAA+TGG - Chr5:101355612-101355631 None:intergenic 20.0%
!!! ATTTTTTAGTTCAACTAACA+TGG + Chr5:101354132-101354151 MsG0580029735.01.T01:intron 20.0%
!!! CAAATGTAGAAAGTTTTTTT+TGG + Chr5:101354638-101354657 MsG0580029735.01.T01:intron 20.0%
!!! TTCATATATACATGATTTTC+AGG + Chr5:101355051-101355070 MsG0580029735.01.T01:intron 20.0%
! AAATTTGACAATGCAATAGA+TGG - Chr5:101355252-101355271 None:intergenic 25.0%
! AACACGATTTAGATAGTTAA+TGG - Chr5:101355033-101355052 None:intergenic 25.0%
! AAGGCTCAAATCAATATATT+TGG + Chr5:101355369-101355388 MsG0580029735.01.T01:intron 25.0%
! AATTTGACAATGCAATAGAT+GGG - Chr5:101355251-101355270 None:intergenic 25.0%
! AGTTTCTTGAAAACATACAA+GGG + Chr5:101354426-101354445 MsG0580029735.01.T01:CDS 25.0%
! ATCTAAATTGTACTCGAATT+TGG - Chr5:101354897-101354916 None:intergenic 25.0%
! ATTCATTATTATCATCTGCA+TGG + Chr5:101354012-101354031 MsG0580029735.01.T01:intron 25.0%
! GTTGCAATGAAAATTATGAT+AGG - Chr5:101355954-101355973 None:intergenic 25.0%
! TAAAGCAATTGAATCCTATA+AGG - Chr5:101354101-101354120 None:intergenic 25.0%
! TGATTATCATCTTTCACATT+GGG + Chr5:101354365-101354384 MsG0580029735.01.T01:intron 25.0%
! TTGATTATCATCTTTCACAT+TGG + Chr5:101354364-101354383 MsG0580029735.01.T01:intron 25.0%
! TTGGTTAATTATGTTTAGCA+TGG - Chr5:101355210-101355229 None:intergenic 25.0%
!! CAAACATTTTGTTGCAATTA+AGG - Chr5:101354269-101354288 None:intergenic 25.0%
!! TTCACTTCTTCTATAGTTTT+TGG + Chr5:101353868-101353887 MsG0580029735.01.T01:intron 25.0%
!!! GTTGATGATGTTCAAAATTA+TGG + Chr5:101355129-101355148 MsG0580029735.01.T01:intron 25.0%
AAACACACACTTCAAAATTG+TGG + Chr5:101355107-101355126 MsG0580029735.01.T01:intron 30.0%
AATGTGAGATACAACTTACA+AGG - Chr5:101353963-101353982 None:intergenic 30.0%
ATTGTTGGAATGGTAGTAAT+TGG - Chr5:101355576-101355595 None:intergenic 30.0%
ATTTGAACACTTGAAAATCG+TGG - Chr5:101355000-101355019 None:intergenic 30.0%
CAGTTTCTTGAAAACATACA+AGG + Chr5:101354425-101354444 MsG0580029735.01.T01:CDS 30.0%
CCAAAGGCCTAATATAATAA+GGG + Chr5:101354576-101354595 MsG0580029735.01.T01:intron 30.0%
GCAAGATCATGAATAGAATT+TGG - Chr5:101355828-101355847 None:intergenic 30.0%
GGTTAATTGTGATCAATGAA+TGG + Chr5:101355150-101355169 MsG0580029735.01.T01:intron 30.0%
TCAAAATCGTGTATGTAAGA+TGG - Chr5:101354967-101354986 None:intergenic 30.0%
TCCAAAGGCCTAATATAATA+AGG + Chr5:101354575-101354594 MsG0580029735.01.T01:intron 30.0%
TGAAACAATAGAGATTAGTG+AGG + Chr5:101354552-101354571 MsG0580029735.01.T01:intron 30.0%
TGCTTTGACTGTTACATATA+GGG + Chr5:101354455-101354474 MsG0580029735.01.T01:CDS 30.0%
TTAGGATTGAAAAATGGATC+AGG - Chr5:101355606-101355625 None:intergenic 30.0%
TTCCTATTCACTAGAAAAGT+TGG - Chr5:101354825-101354844 None:intergenic 30.0%
! CAAATTTTCTACTGTCCAAA+AGG + Chr5:101355539-101355558 MsG0580029735.01.T01:CDS 30.0%
! TACAAGATGCTTTTCCTTAT+AGG + Chr5:101354084-101354103 MsG0580029735.01.T01:intron 30.0%
! TTTCAAATTCAGGTTCAAGT+AGG + Chr5:101355445-101355464 MsG0580029735.01.T01:CDS 30.0%
!! ATATTGATTTGAGCCTTAAG+TGG - Chr5:101355366-101355385 None:intergenic 30.0%
!! ATTTTGTATAGAGCATCTTC+TGG - Chr5:101354522-101354541 None:intergenic 30.0%
!! GTTCATTTTTATGCCACTTA+AGG + Chr5:101355350-101355369 MsG0580029735.01.T01:intron 30.0%
!!! AGGAAGACAGTTTTTTGATT+TGG + Chr5:101354840-101354859 MsG0580029735.01.T01:intron 30.0%
!!! TTGGATGCAATGTACTTTTT+TGG + Chr5:101354177-101354196 MsG0580029735.01.T01:intron 30.0%
ACAAGTTAAGGAGCTTGAAA+AGG - Chr5:101354732-101354751 None:intergenic 35.0%
ACTGTGTCTGGATTGTAATT+AGG - Chr5:101355624-101355643 None:intergenic 35.0%
ATGAGTTACAAAGTTGTCTC+TGG + Chr5:101353917-101353936 MsG0580029735.01.T01:intron 35.0%
ATGATAGGAAAAGCCTTACT+TGG - Chr5:101355939-101355958 None:intergenic 35.0%
ATTGGACAATAGAGACATGT+GGG - Chr5:101354499-101354518 None:intergenic 35.0%
CCCTTATTATATTAGGCCTT+TGG - Chr5:101354579-101354598 None:intergenic 35.0%
CTGCTTTGACTGTTACATAT+AGG + Chr5:101354454-101354473 MsG0580029735.01.T01:CDS 35.0%
GAAAAAAATCACCTGAGCAA+AGG - Chr5:101353848-101353867 None:intergenic 35.0%
GCTTTGACTGTTACATATAG+GGG + Chr5:101354456-101354475 MsG0580029735.01.T01:CDS 35.0%
TATGTAACAGTCAAAGCAGT+TGG - Chr5:101354453-101354472 None:intergenic 35.0%
TTAGACTATGCTTGTGGATA+TGG + Chr5:101355756-101355775 MsG0580029735.01.T01:CDS 35.0%
TTTGTTAACTTATGCCCCAA+GGG - Chr5:101354617-101354636 None:intergenic 35.0%
! AAATTGCAGCTATTTGGAAC+AGG - Chr5:101355885-101355904 None:intergenic 35.0%
! AGCAAAGGATTTGAGTTAGT+AGG - Chr5:101353833-101353852 None:intergenic 35.0%
! AGGTGTTGTAATTGTTGGAA+TGG - Chr5:101355586-101355605 None:intergenic 35.0%
! ATTCTTTTCCAGGTTCAAGT+TGG + Chr5:101355688-101355707 MsG0580029735.01.T01:CDS 35.0%
! GTTCCAAATAGCTGCAATTT+TGG + Chr5:101355885-101355904 MsG0580029735.01.T01:CDS 35.0%
! TCATATCAGAACAGTACTGA+AGG - Chr5:101353901-101353920 None:intergenic 35.0%
! TTGGAGTGGTAATATCCTTT+TGG - Chr5:101355557-101355576 None:intergenic 35.0%
!! AAATGTTAACTTGTGCCCTT+GGG + Chr5:101354599-101354618 MsG0580029735.01.T01:intron 35.0%
!! CTCCAACTTTTCTAGTGAAT+AGG + Chr5:101354820-101354839 MsG0580029735.01.T01:intron 35.0%
!! GTATATGGAAGTGTTGAAGT+TGG - Chr5:101355651-101355670 None:intergenic 35.0%
!! TGTTTTCAAGAAACTGCATG+TGG - Chr5:101354421-101354440 None:intergenic 35.0%
!! TTTGAGTTGAGTTTCATGCA+AGG + Chr5:101355295-101355314 MsG0580029735.01.T01:intron 35.0%
ACCTGGAAAAGAATTCACTG+TGG - Chr5:101355682-101355701 None:intergenic 40.0%
AGAATTCACTGTGGTTGGAT+TGG - Chr5:101355673-101355692 None:intergenic 40.0%
AGGTTCAAGTAGGACAGAAA+AGG + Chr5:101355455-101355474 MsG0580029735.01.T01:CDS 40.0%
CACTAGCACTAATCCAAGTA+AGG + Chr5:101355923-101355942 MsG0580029735.01.T01:CDS 40.0%
CCACCAAAATTGCAGCTATT+TGG - Chr5:101355891-101355910 None:intergenic 40.0%
CTAACTCAAATCCTTTGCTC+AGG + Chr5:101353834-101353853 MsG0580029735.01.T01:CDS 40.0%
CTTTGTTAACTTATGCCCCA+AGG - Chr5:101354618-101354637 None:intergenic 40.0%
GATTGGACAATAGAGACATG+TGG - Chr5:101354500-101354519 None:intergenic 40.0%
GATTGTTCAGCAATTCAACC+TGG + Chr5:101355786-101355805 MsG0580029735.01.T01:CDS 40.0%
GGAAAAGAATTCACTGTGGT+TGG - Chr5:101355678-101355697 None:intergenic 40.0%
GTTGCATTAGACTATGCTTG+TGG + Chr5:101355750-101355769 MsG0580029735.01.T01:CDS 40.0%
TAGAGATTAGTGAGGTCCAA+AGG + Chr5:101354560-101354579 MsG0580029735.01.T01:intron 40.0%
TGGAATGGTAGTAATTGGAG+TGG - Chr5:101355571-101355590 None:intergenic 40.0%
TTGGACAATAGAGACATGTG+GGG - Chr5:101354498-101354517 None:intergenic 40.0%
! ACCACAGTGAATTCTTTTCC+AGG + Chr5:101355678-101355697 MsG0580029735.01.T01:CDS 40.0%
! CCAAATAGCTGCAATTTTGG+TGG + Chr5:101355888-101355907 MsG0580029735.01.T01:CDS 40.0%
! GGATCAGGTGTTGTAATTGT+TGG - Chr5:101355591-101355610 None:intergenic 40.0%
! GTATAGAGCATCTTCTGGAT+TGG - Chr5:101354517-101354536 None:intergenic 40.0%
!! AATGTTAACTTGTGCCCTTG+GGG + Chr5:101354600-101354619 MsG0580029735.01.T01:intron 40.0%
!! ACTGTGGTTGGATTGGTATA+TGG - Chr5:101355666-101355685 None:intergenic 40.0%
!! ATGTGGAAGTACTGGAGAAA+AGG - Chr5:101354404-101354423 None:intergenic 40.0%
!! CATGTTAACTTGTGCCCTTA+GGG - Chr5:101354756-101354775 None:intergenic 40.0%
!! GAAATGTTAACTTGTGCCCT+TGG + Chr5:101354598-101354617 MsG0580029735.01.T01:intron 40.0%
!! TCATGTTAACTTGTGCCCTT+AGG - Chr5:101354757-101354776 None:intergenic 40.0%
AAACCAATTTGCGACGCACT+TGG - Chr5:101355726-101355745 None:intergenic 45.0%
ATATAGGGGTCTCAGTACAG+AGG + Chr5:101354470-101354489 MsG0580029735.01.T01:CDS 45.0%
GAAGTTGGAGAAACTGTGTC+TGG - Chr5:101355636-101355655 None:intergenic 45.0%
GACTATGCTTGTGGATATGG+TGG + Chr5:101355759-101355778 MsG0580029735.01.T01:CDS 45.0%
TGTTCAGCAATTCAACCTGG+TGG + Chr5:101355789-101355808 MsG0580029735.01.T01:CDS 45.0%
! GAAACTGCATGTGGAAGTAC+TGG - Chr5:101354412-101354431 None:intergenic 45.0%
!! CTCCTTAACTTGTGCCCTAA+GGG + Chr5:101354739-101354758 MsG0580029735.01.T01:intron 45.0%
!! GGAAGTACTGGAGAAAAGGT+TGG - Chr5:101354400-101354419 None:intergenic 45.0%
!!! TTCTTTTATATTTATTATTT+TGG + Chr5:101354041-101354060 MsG0580029735.01.T01:intron 5.0%
AGTCCAAGTGCGTCGCAAAT+TGG + Chr5:101355720-101355739 MsG0580029735.01.T01:CDS 50.0%
GCAACACACCAACTTGAACC+TGG - Chr5:101355699-101355718 None:intergenic 50.0%
GGATTGTAACAGCTTCCACC+AGG - Chr5:101355807-101355826 None:intergenic 50.0%
TGCCCTTAGGGCACAAGTTA+AGG - Chr5:101354744-101354763 None:intergenic 50.0%
!! GCTCCTTAACTTGTGCCCTA+AGG + Chr5:101354738-101354757 MsG0580029735.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 101353795 101356073 101353795 ID=MsG0580029735.01;Name=MsG0580029735.01
Chr5 mRNA 101353795 101356073 101353795 ID=MsG0580029735.01.T01;Parent=MsG0580029735.01;Name=MsG0580029735.01.T01;_AED=0.40;_eAED=0.54;_QI=0|0|0|1|0|0|3|0|273
Chr5 exon 101353795 101353855 101353795 ID=MsG0580029735.01.T01:exon:26709;Parent=MsG0580029735.01.T01
Chr5 exon 101354391 101354491 101354391 ID=MsG0580029735.01.T01:exon:26710;Parent=MsG0580029735.01.T01
Chr5 exon 101355414 101356073 101355414 ID=MsG0580029735.01.T01:exon:26711;Parent=MsG0580029735.01.T01
Chr5 CDS 101353795 101353855 101353795 ID=MsG0580029735.01.T01:cds;Parent=MsG0580029735.01.T01
Chr5 CDS 101354391 101354491 101354391 ID=MsG0580029735.01.T01:cds;Parent=MsG0580029735.01.T01
Chr5 CDS 101355414 101356073 101355414 ID=MsG0580029735.01.T01:cds;Parent=MsG0580029735.01.T01
Gene Sequence

>MsG0580029735.01.T01

ATGAAAAGGAGTGTATATCACTATGCAATTTTTCTCCTACTAACTCAAATCCTTTGCTCAGTTGAATCCAACCTTTTCTCCAGTACTTCCACATGCAGTTTCTTGAAAACATACAAGGGTCCAACTGCTTTGACTGTTACATATAGGGGTCTCAGTACAGAGAATCAGAACAGACAAAGTAATTCAATTATGATTTCAAATTCAGGTTCAAGTAGGACAGAAAAGGAAGCACAAAGACATGTTAATGTACTACAAAGACATGAAGTGAAACAAGAATTTATTACTGACAAATTTTCTACTGTCCAAAAGGATATTACCACTCCAATTACTACCATTCCAACAATTACAACACCTGATCCATTTTTCAATCCTAATTACAATCCAGACACAGTTTCTCCAACTTCAACACTTCCATATACCAATCCAACCACAGTGAATTCTTTTCCAGGTTCAAGTTGGTGTGTTGCAAGTCCAAGTGCGTCGCAAATTGGTTTACAAGTTGCATTAGACTATGCTTGTGGATATGGTGGCACTGATTGTTCAGCAATTCAACCTGGTGGAAGCTGTTACAATCCAAATTCTATTCATGATCTTGCTTCTTATGCCTTTAATAAGTATTATCATAAGAATCCTGTTCCAAATAGCTGCAATTTTGGTGGAACTGCTGTCATCACTAGCACTAATCCAAGTAAGGCTTTTCCTATCATAATTTTCATTGCAACCTTTACAATAATATTAATTGCATCCTTTAGTTATATCAAATTCAAAAGCATGCATATGTTGTTAATTAGATTGAGAATGATAGAAAATATAAACCCTTAA
Protein sequence

>MsG0580029735.01.T01

MKRSVYHYAIFLLLTQILCSVESNLFSSTSTCSFLKTYKGPTALTVTYRGLSTENQNRQSNSIMISNSGSSRTEKEAQRHVNVLQRHEVKQEFITDKFSTVQKDITTPITTIPTITTPDPFFNPNYNPDTVSPTSTLPYTNPTTVNSFPGSSWCVASPSASQIGLQVALDYACGYGGTDCSAIQPGGSCYNPNSIHDLASYAFNKYYHKNPVPNSCNFGGTAVITSTNPSKAFPIIIFIATFTIILIASFSYIKFKSMHMLLIRLRMIENINP*