Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029886.01.T01 | GAU19045.1 | 63.333 | 60 | 22 | 0 | 6 | 65 | 1 | 60 | 6.48E-17 | 81.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029886.01.T01 | A0A2Z6MS45 | 63.333 | 60 | 22 | 0 | 6 | 65 | 1 | 60 | 3.09e-17 | 81.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021832.01 | MsG0580029886.01 | 0.803976 | 2.696218e-49 | 1.373809e-46 |
| MsG0580029886.01 | MsG0780039340.01 | 0.806165 | 9.391026e-50 | 5.062054e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029886.01.T01 | MTR_5g070620 | 45.833 | 72 | 39 | 0 | 1 | 72 | 21 | 92 | 2.31e-19 | 75.1 |
| MsG0580029886.01.T01 | MTR_8g059025 | 64.000 | 50 | 18 | 0 | 6 | 55 | 1 | 50 | 2.55e-18 | 73.6 |
| MsG0580029886.01.T01 | MTR_8g467450 | 64.000 | 50 | 18 | 0 | 6 | 55 | 1 | 50 | 3.75e-18 | 73.2 |
| MsG0580029886.01.T01 | MTR_5g078250 | 61.224 | 49 | 19 | 0 | 6 | 54 | 1 | 49 | 1.42e-15 | 69.7 |
| MsG0580029886.01.T01 | MTR_8g062030 | 56.250 | 48 | 21 | 0 | 6 | 53 | 1 | 48 | 1.11e-14 | 67.0 |
| MsG0580029886.01.T01 | MTR_1g075850 | 59.091 | 44 | 18 | 0 | 6 | 49 | 1 | 44 | 7.27e-14 | 64.7 |
| MsG0580029886.01.T01 | MTR_6g051890 | 43.243 | 74 | 41 | 1 | 3 | 75 | 2 | 75 | 8.01e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACCATCTCTATGGAAT+TGG | 0.309307 | 5:-103413334 | None:intergenic |
| ATCAATGAGAACCATCTCTA+TGG | 0.370825 | 5:-103413340 | None:intergenic |
| AGGTAAGGGTTCTGAGGTTG+TGG | 0.428800 | 5:+103413280 | MsG0580029886.01.T01:CDS |
| TCTATGGAATTGGGCTGCTC+AGG | 0.436111 | 5:-103413324 | None:intergenic |
| GATGGTTCTCATTGATGATA+AGG | 0.446818 | 5:+103413347 | MsG0580029886.01.T01:CDS |
| TATCACCTGGTAGGGAGAAA+TGG | 0.447319 | 5:+103413250 | MsG0580029886.01.T01:CDS |
| AGAACCATCTCTATGGAATT+GGG | 0.470154 | 5:-103413333 | None:intergenic |
| AGAAATGGTGGCTTAAGGTA+AGG | 0.470561 | 5:+103413265 | MsG0580029886.01.T01:CDS |
| TGCTCAGGATTCAAGAAGCT+TGG | 0.480765 | 5:-103413309 | None:intergenic |
| GGTAAGGGTTCTGAGGTTGT+GGG | 0.499506 | 5:+103413281 | MsG0580029886.01.T01:CDS |
| AGCCACCATTTCTCCCTACC+AGG | 0.509535 | 5:-103413255 | None:intergenic |
| GGCTTAAGGTAAGGGTTCTG+AGG | 0.522560 | 5:+103413274 | MsG0580029886.01.T01:CDS |
| TGTCCGCTGTATCACCTGGT+AGG | 0.525200 | 5:+103413241 | MsG0580029886.01.T01:CDS |
| TAGGGAGAAATGGTGGCTTA+AGG | 0.528302 | 5:+103413260 | MsG0580029886.01.T01:CDS |
| GAAATGGTGGCTTAAGGTAA+GGG | 0.561830 | 5:+103413266 | MsG0580029886.01.T01:CDS |
| AAGGGTTCTGAGGTTGTGGG+AGG | 0.577564 | 5:+103413284 | MsG0580029886.01.T01:CDS |
| TCAGTGTCCGCTGTATCACC+TGG | 0.580487 | 5:+103413237 | MsG0580029886.01.T01:CDS |
| GTCCGCTGTATCACCTGGTA+GGG | 0.583124 | 5:+103413242 | MsG0580029886.01.T01:CDS |
| GCAGCCCAATTCCATAGAGA+TGG | 0.623094 | 5:+103413329 | MsG0580029886.01.T01:CDS |
| CACCTGGTAGGGAGAAATGG+TGG | 0.663854 | 5:+103413253 | MsG0580029886.01.T01:CDS |
| CTCCCTACCAGGTGATACAG+CGG | 0.675902 | 5:-103413244 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTTATTAGTTTATCACT+TGG | + | Chr5:103413401-103413420 | MsG0580029886.01.T01:CDS | 20.0% |
| AGAACCATCTCTATGGAATT+GGG | - | Chr5:103413336-103413355 | None:intergenic | 35.0% | |
| ATCAATGAGAACCATCTCTA+TGG | - | Chr5:103413343-103413362 | None:intergenic | 35.0% | |
| ! | GATGGTTCTCATTGATGATA+AGG | + | Chr5:103413347-103413366 | MsG0580029886.01.T01:CDS | 35.0% |
| AGAAATGGTGGCTTAAGGTA+AGG | + | Chr5:103413265-103413284 | MsG0580029886.01.T01:CDS | 40.0% | |
| GAAATGGTGGCTTAAGGTAA+GGG | + | Chr5:103413266-103413285 | MsG0580029886.01.T01:CDS | 40.0% | |
| GAGAACCATCTCTATGGAAT+TGG | - | Chr5:103413337-103413356 | None:intergenic | 40.0% | |
| TAGGGAGAAATGGTGGCTTA+AGG | + | Chr5:103413260-103413279 | MsG0580029886.01.T01:CDS | 45.0% | |
| TATCACCTGGTAGGGAGAAA+TGG | + | Chr5:103413250-103413269 | MsG0580029886.01.T01:CDS | 45.0% | |
| TGCTCAGGATTCAAGAAGCT+TGG | - | Chr5:103413312-103413331 | None:intergenic | 45.0% | |
| GCAGCCCAATTCCATAGAGA+TGG | + | Chr5:103413329-103413348 | MsG0580029886.01.T01:CDS | 50.0% | |
| GGCTTAAGGTAAGGGTTCTG+AGG | + | Chr5:103413274-103413293 | MsG0580029886.01.T01:CDS | 50.0% | |
| TCTATGGAATTGGGCTGCTC+AGG | - | Chr5:103413327-103413346 | None:intergenic | 50.0% | |
| !! | AGGTAAGGGTTCTGAGGTTG+TGG | + | Chr5:103413280-103413299 | MsG0580029886.01.T01:CDS | 50.0% |
| !! | GGTAAGGGTTCTGAGGTTGT+GGG | + | Chr5:103413281-103413300 | MsG0580029886.01.T01:CDS | 50.0% |
| AGCCACCATTTCTCCCTACC+AGG | - | Chr5:103413258-103413277 | None:intergenic | 55.0% | |
| CACCTGGTAGGGAGAAATGG+TGG | + | Chr5:103413253-103413272 | MsG0580029886.01.T01:CDS | 55.0% | |
| CTCCCTACCAGGTGATACAG+CGG | - | Chr5:103413247-103413266 | None:intergenic | 55.0% | |
| GTCCGCTGTATCACCTGGTA+GGG | + | Chr5:103413242-103413261 | MsG0580029886.01.T01:CDS | 55.0% | |
| TCAGTGTCCGCTGTATCACC+TGG | + | Chr5:103413237-103413256 | MsG0580029886.01.T01:CDS | 55.0% | |
| TGTCCGCTGTATCACCTGGT+AGG | + | Chr5:103413241-103413260 | MsG0580029886.01.T01:CDS | 55.0% | |
| !! | AAGGGTTCTGAGGTTGTGGG+AGG | + | Chr5:103413284-103413303 | MsG0580029886.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 103413204 | 103413431 | 103413204 | ID=MsG0580029886.01;Name=MsG0580029886.01 |
| Chr5 | mRNA | 103413204 | 103413431 | 103413204 | ID=MsG0580029886.01.T01;Parent=MsG0580029886.01;Name=MsG0580029886.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|75 |
| Chr5 | exon | 103413204 | 103413431 | 103413204 | ID=MsG0580029886.01.T01:exon:7077;Parent=MsG0580029886.01.T01 |
| Chr5 | CDS | 103413204 | 103413431 | 103413204 | ID=MsG0580029886.01.T01:cds;Parent=MsG0580029886.01.T01 |
| Gene Sequence |
| Protein sequence |
