AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030009.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030009.01.T01 MTR_5g096560 90.345 145 13 1 1 144 1 145 9.88e-94 273
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCCTACGGCACAAATTT+TGG 0.150016 5:-105047123 MsG0580030009.01.T01:five_prime_UTR
AAGATAAATGATGGGAAAAT+TGG 0.155313 5:-105046312 MsG0580030009.01.T01:CDS
TTAGTTCGTGTGTTGGTGTT+AGG 0.315723 5:-105046410 MsG0580030009.01.T01:CDS
TTAGGAACATTTGGAAGTTT+CGG 0.323492 5:-105046458 MsG0580030009.01.T01:intron
GTATGAACTTCATCTGGATT+TGG 0.326325 5:+105046258 None:intergenic
GGTTGGAGATTCTTGTGAAC+AGG 0.371729 5:-105044855 MsG0580030009.01.T01:CDS
AGCCGATTGTTCAGCATCAT+TGG 0.373844 5:+105044808 None:intergenic
TTCACCCTGCATGATGTGTT+AGG 0.382312 5:+105046381 None:intergenic
AAATTGAAATTAGGAACATT+TGG 0.383207 5:-105046467 MsG0580030009.01.T01:intron
CAAAGCTACAATACTCCAAA+TGG 0.386271 5:+105046352 None:intergenic
CAGGGTGAAAATTCCCCATT+TGG 0.401072 5:-105046367 MsG0580030009.01.T01:CDS
AACTCAGTATGAACTTCATC+TGG 0.406135 5:+105046252 None:intergenic
AGATAAATGATGGGAAAATT+GGG 0.425380 5:-105046311 MsG0580030009.01.T01:CDS
CCAAAATTTGTGCCGTAGGA+AGG 0.431418 5:+105047123 None:intergenic
GATTCTAGGTTCTGAACAAA+TGG 0.438649 5:-105044882 MsG0580030009.01.T01:intron
CTCTTTGATGATAGTGAAGA+AGG 0.444371 5:-105046228 MsG0580030009.01.T01:intron
AAAGCTACAATACTCCAAAT+GGG 0.474481 5:+105046353 None:intergenic
TACCAATGATGCTGAACAAT+CGG 0.477064 5:-105044810 MsG0580030009.01.T01:CDS
AGTGTAATCAAGATAAATGA+TGG 0.484873 5:-105046321 MsG0580030009.01.T01:CDS
GCTGTCAATTACTTGCATCT+AGG 0.497614 5:-105044788 MsG0580030009.01.T01:CDS
CAAAATTTGTGCCGTAGGAA+GGG 0.500964 5:+105047124 None:intergenic
AGAGACTTTAGTTCGTGTGT+TGG 0.535118 5:-105046417 MsG0580030009.01.T01:CDS
AGAAGTTTAGCGGGAAACAT+TGG 0.537917 5:-105047084 MsG0580030009.01.T01:CDS
AGGTTCTGAACAAATGGAAG+AGG 0.550123 5:-105044876 MsG0580030009.01.T01:CDS
TCTGAACAAATGGAAGAGGT+TGG 0.556064 5:-105044872 MsG0580030009.01.T01:CDS
AACATGGCAAGAAGTTTAGC+GGG 0.560092 5:-105047093 MsG0580030009.01.T01:CDS
ACATTCCTAACACATCATGC+AGG 0.560654 5:-105046386 MsG0580030009.01.T01:CDS
GTAACCAAAATTTGTGCCGT+AGG 0.564041 5:+105047119 None:intergenic
GAGTATTGTAGCTTTGCTGA+TGG 0.583649 5:-105046345 MsG0580030009.01.T01:CDS
TTCGGATTGAATGCTTCAAG+AGG 0.601314 5:-105046440 MsG0580030009.01.T01:CDS
GAACATGGCAAGAAGTTTAG+CGG 0.604379 5:-105047094 MsG0580030009.01.T01:CDS
GTGTAATCAAGATAAATGAT+GGG 0.609791 5:-105046320 MsG0580030009.01.T01:CDS
GAGAGTAGAGACCCTTCCTA+CGG 0.642738 5:-105047135 MsG0580030009.01.T01:five_prime_UTR
CATTCCTAACACATCATGCA+GGG 0.662603 5:-105046385 MsG0580030009.01.T01:CDS
AAGCTACAATACTCCAAATG+GGG 0.674118 5:+105046354 None:intergenic
TTTGGAGAAGTTTCACAATG+CGG 0.677880 5:+105046276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTGAAATTGAAATT+AGG - Chr5:105045468-105045487 MsG0580030009.01.T01:intron 10.0%
!!! AACAAAATTTGATAATGTTA+CGG - Chr5:105046698-105046717 MsG0580030009.01.T01:intron 15.0%
!!! ACTTAATGCTAAAATTTTAA+AGG + Chr5:105046881-105046900 None:intergenic 15.0%
!!! CATAATTTTTTTGAGATAAA+TGG + Chr5:105044955-105044974 None:intergenic 15.0%
!!! TTTCAGCTTTTAATTAATAA+CGG - Chr5:105045151-105045170 MsG0580030009.01.T01:intron 15.0%
!! AAATAAGTTGTTTATCCAAA+CGG - Chr5:105045371-105045390 MsG0580030009.01.T01:intron 20.0%
!! AAATTGAAATTAGGAACATT+TGG - Chr5:105045477-105045496 MsG0580030009.01.T01:intron 20.0%
!! AAGCATCAAATTTAATTACA+AGG + Chr5:105046159-105046178 None:intergenic 20.0%
!! ATAAAATAGAATGATGTCAT+AGG + Chr5:105045864-105045883 None:intergenic 20.0%
!! ATCCTAGATATTAATGATTA+AGG + Chr5:105046800-105046819 None:intergenic 20.0%
!! ATTCAACTTATCAAATACAA+TGG + Chr5:105046664-105046683 None:intergenic 20.0%
!! GACAAAAACATTACAAAATA+TGG + Chr5:105045399-105045418 None:intergenic 20.0%
!! TATATAAAATAGTAGTACCT+GGG + Chr5:105044929-105044948 None:intergenic 20.0%
!! TTAATCATTAATATCTAGGA+TGG - Chr5:105046799-105046818 MsG0580030009.01.T01:intron 20.0%
!! TTATATAAAATAGTAGTACC+TGG + Chr5:105044930-105044949 None:intergenic 20.0%
!! TTGTAATTAAATTTGATGCT+TGG - Chr5:105046158-105046177 MsG0580030009.01.T01:intron 20.0%
!!! AAAGGAAAAAAGTATATCTT+AGG - Chr5:105046736-105046755 MsG0580030009.01.T01:intron 20.0%
!!! AAGGAAAAAAGTATATCTTA+GGG - Chr5:105046737-105046756 MsG0580030009.01.T01:intron 20.0%
!!! ACAAAATTTGATAATGTTAC+GGG - Chr5:105046699-105046718 MsG0580030009.01.T01:intron 20.0%
!!! TGCTAAAATTTTAAAGGTAA+TGG + Chr5:105046875-105046894 None:intergenic 20.0%
!!! TTTTGTATATGTATAAAGAC+TGG - Chr5:105046581-105046600 MsG0580030009.01.T01:intron 20.0%
! AAATAACCGACCTATAATAA+AGG - Chr5:105045980-105045999 MsG0580030009.01.T01:intron 25.0%
! AATAAGTTGTTTATCCAAAC+GGG - Chr5:105045372-105045391 MsG0580030009.01.T01:intron 25.0%
! AATCATGACCAAAAACATTA+TGG + Chr5:105045428-105045447 None:intergenic 25.0%
! AATGGAAAAACAAATGAACA+TGG + Chr5:105046857-105046876 None:intergenic 25.0%
! ACATTAATACTACCAATTCA+TGG - Chr5:105046100-105046119 MsG0580030009.01.T01:intron 25.0%
! AGTGTAATCAAGATAAATGA+TGG - Chr5:105045623-105045642 MsG0580030009.01.T01:intron 25.0%
! ATATAAAATAGTAGTACCTG+GGG + Chr5:105044928-105044947 None:intergenic 25.0%
! ATTAATACTACCAATTCATG+GGG - Chr5:105046102-105046121 MsG0580030009.01.T01:intron 25.0%
! CATTAATACTACCAATTCAT+GGG - Chr5:105046101-105046120 MsG0580030009.01.T01:intron 25.0%
! CTCCTTAATCATTAATATCT+AGG - Chr5:105046795-105046814 MsG0580030009.01.T01:intron 25.0%
! GAAGTTATAACTTATCTATG+TGG - Chr5:105046261-105046280 MsG0580030009.01.T01:CDS 25.0%
! GAGAACATTCTCTAAATAAA+TGG + Chr5:105046613-105046632 None:intergenic 25.0%
! GTGTAATCAAGATAAATGAT+GGG - Chr5:105045624-105045643 MsG0580030009.01.T01:intron 25.0%
! TCGGTTAATCATAGTTTAAA+TGG + Chr5:105046543-105046562 None:intergenic 25.0%
! TTCAACTATAGCAAACAAAT+GGG + Chr5:105044979-105044998 None:intergenic 25.0%
! TTTCAACTATAGCAAACAAA+TGG + Chr5:105044980-105044999 None:intergenic 25.0%
!! AAACACGGATTTTATCATTT+TGG - Chr5:105046046-105046065 MsG0580030009.01.T01:intron 25.0%
!! AACACGGATTTTATCATTTT+GGG - Chr5:105046047-105046066 MsG0580030009.01.T01:intron 25.0%
!! AAGATAAATGATGGGAAAAT+TGG - Chr5:105045632-105045651 MsG0580030009.01.T01:intron 25.0%
!! AATCAAACTTTTCAGTGATT+TGG + Chr5:105045005-105045024 None:intergenic 25.0%
!! AGATAAATGATGGGAAAATT+GGG - Chr5:105045633-105045652 MsG0580030009.01.T01:intron 25.0%
!! ATCAGAACAACTTTATTTCT+TGG + Chr5:105047028-105047047 None:intergenic 25.0%
!! TCATGATTCCATAATGTTTT+TGG - Chr5:105045417-105045436 MsG0580030009.01.T01:intron 25.0%
!! TGTTTTCAAAACTGAATGAA+TGG + Chr5:105046031-105046050 None:intergenic 25.0%
!!! AAATTTTGGTTACATGAACA+TGG - Chr5:105044835-105044854 MsG0580030009.01.T01:CDS 25.0%
!!! AGATATTTGTTTTATCTCAC+GGG - Chr5:105046393-105046412 MsG0580030009.01.T01:CDS 25.0%
!!! CAAAATTTGATAATGTTACG+GGG - Chr5:105046700-105046719 MsG0580030009.01.T01:intron 25.0%
!!! GAGATATTTGTTTTATCTCA+CGG - Chr5:105046392-105046411 MsG0580030009.01.T01:CDS 25.0%
!!! GCTCTAGAGAAAATTAATAA+TGG + Chr5:105046765-105046784 None:intergenic 25.0%
!!! TAGTTCGGTTTTAAAAACAT+TGG + Chr5:105045887-105045906 None:intergenic 25.0%
!!! TTCATTCAGTTTTGAAAACA+CGG - Chr5:105046031-105046050 MsG0580030009.01.T01:intron 25.0%
AAAGCTACAATACTCCAAAT+GGG + Chr5:105045594-105045613 None:intergenic 30.0%
AACCAATTGGGTATCTAAAT+TGG + Chr5:105045057-105045076 None:intergenic 30.0%
ACAGATCAAGTGTATAAACT+AGG + Chr5:105046346-105046365 None:intergenic 30.0%
AGAAGTTAATGAGCTAAAAC+AGG + Chr5:105046916-105046935 None:intergenic 30.0%
AGTTTATACACTTGATCTGT+AGG - Chr5:105046346-105046365 MsG0580030009.01.T01:CDS 30.0%
ATAAGTTGTTTATCCAAACG+GGG - Chr5:105045373-105045392 MsG0580030009.01.T01:intron 30.0%
ATCTATGTGGTGAGTTTATT+AGG - Chr5:105046274-105046293 MsG0580030009.01.T01:CDS 30.0%
ATCTTTAACAGTTTAGCACT+CGG + Chr5:105046562-105046581 None:intergenic 30.0%
ATTAAATTTGATGCTTGGCA+CGG - Chr5:105046163-105046182 MsG0580030009.01.T01:intron 30.0%
CAGATCAAGTGTATAAACTA+GGG + Chr5:105046345-105046364 None:intergenic 30.0%
CCAAACTTGGATTAACTTAA+TGG + Chr5:105045841-105045860 None:intergenic 30.0%
CCATTAAGTTAATCCAAGTT+TGG - Chr5:105045838-105045857 MsG0580030009.01.T01:intron 30.0%
GGAAAATAGTCAACTTTCTA+GGG + Chr5:105046522-105046541 None:intergenic 30.0%
TGGAAAATAGTCAACTTTCT+AGG + Chr5:105046523-105046542 None:intergenic 30.0%
TTAGGAACATTTGGAAGTTT+CGG - Chr5:105045486-105045505 MsG0580030009.01.T01:intron 30.0%
TTCAAATTTAGCTACCCAAT+TGG - Chr5:105045176-105045195 MsG0580030009.01.T01:intron 30.0%
TTCCAATTTAGATACCCAAT+TGG - Chr5:105045052-105045071 MsG0580030009.01.T01:intron 30.0%
! ACACGGATTTTATCATTTTG+GGG - Chr5:105046048-105046067 MsG0580030009.01.T01:intron 30.0%
! GGAGATATGCTTATAGTTTT+TGG + Chr5:105046324-105046343 None:intergenic 30.0%
! GTTCTGATCATCTTAATTTG+TGG - Chr5:105047040-105047059 MsG0580030009.01.T01:CDS 30.0%
! TTATGGCTGACTATACATTT+TGG - Chr5:105046469-105046488 MsG0580030009.01.T01:intron 30.0%
!! ATAAGTTTTGTGAGACTGTT+TGG - Chr5:105045275-105045294 MsG0580030009.01.T01:intron 30.0%
!! CATCTTAATTTGTGGATTCT+AGG - Chr5:105047048-105047067 MsG0580030009.01.T01:CDS 30.0%
!! TAAGTTTTGTGAGACTGTTT+GGG - Chr5:105045276-105045295 MsG0580030009.01.T01:intron 30.0%
!!! AATTTTCTCTAGAGCTTGAT+AGG - Chr5:105046770-105046789 MsG0580030009.01.T01:intron 30.0%
!!! TTATAGGTCGGTTATTTTTG+CGG + Chr5:105045977-105045996 None:intergenic 30.0%
AACCGACCTATAATAAAGGT+CGG - Chr5:105045984-105046003 MsG0580030009.01.T01:intron 35.0%
AACCGACCTTTATTATAGGT+CGG + Chr5:105045989-105046008 None:intergenic 35.0%
AACTCAGTATGAACTTCATC+TGG + Chr5:105045695-105045714 None:intergenic 35.0%
AACTGAATGAATGGATCAAC+CGG + Chr5:105046022-105046041 None:intergenic 35.0%
AAGCTACAATACTCCAAATG+GGG + Chr5:105045593-105045612 None:intergenic 35.0%
AGACACACATAGATAACCAT+CGG - Chr5:105046438-105046457 MsG0580030009.01.T01:CDS 35.0%
CAAAGCTACAATACTCCAAA+TGG + Chr5:105045595-105045614 None:intergenic 35.0%
CCAATTGGTTGCATTGTAAT+GGG - Chr5:105045067-105045086 MsG0580030009.01.T01:intron 35.0%
CCATTACAATGCAACCAATT+GGG + Chr5:105045069-105045088 None:intergenic 35.0%
CTTGAACCGACCTTTATTAT+AGG + Chr5:105045993-105046012 None:intergenic 35.0%
GTATGAACTTCATCTGGATT+TGG + Chr5:105045689-105045708 None:intergenic 35.0%
TACCAATGATGCTGAACAAT+CGG - Chr5:105047134-105047153 MsG0580030009.01.T01:five_prime_UTR 35.0%
TCTCACAAAACTTATGAGAG+TGG + Chr5:105045270-105045289 None:intergenic 35.0%
TTAAATTTGATGCTTGGCAC+GGG - Chr5:105046164-105046183 MsG0580030009.01.T01:intron 35.0%
TTTGGAGAAGTTTCACAATG+CGG + Chr5:105045671-105045690 None:intergenic 35.0%
! AATAGTCAACTTTCTAGGGA+TGG + Chr5:105046518-105046537 None:intergenic 35.0%
! CATACTGGTACTTTGGTAAT+TGG + Chr5:105046233-105046252 None:intergenic 35.0%
! CTATAATAAAGGTCGGTTCA+AGG - Chr5:105045991-105046010 MsG0580030009.01.T01:intron 35.0%
! CTCTTTGATGATAGTGAAGA+AGG - Chr5:105045716-105045735 MsG0580030009.01.T01:intron 35.0%
!! AATTTGATAATGTTACGGGG+AGG - Chr5:105046703-105046722 MsG0580030009.01.T01:intron 35.0%
!! GATTCTAGGTTCTGAACAAA+TGG - Chr5:105047062-105047081 MsG0580030009.01.T01:CDS 35.0%
!!! GCTTTTAGTTCGCTTATTTG+AGG - Chr5:105045240-105045259 MsG0580030009.01.T01:intron 35.0%
AACATGGCAAGAAGTTTAGC+GGG - Chr5:105044851-105044870 MsG0580030009.01.T01:CDS 40.0%
AACCATCGGAGCTTACTTTA+TGG - Chr5:105046452-105046471 MsG0580030009.01.T01:CDS 40.0%
AATGTTACGGGGAGGAAATT+GGG - Chr5:105046711-105046730 MsG0580030009.01.T01:intron 40.0%
ACATTCCTAACACATCATGC+AGG - Chr5:105045558-105045577 MsG0580030009.01.T01:intron 40.0%
ACTTATGCTTCACTTCACAG+AGG - Chr5:105046073-105046092 MsG0580030009.01.T01:intron 40.0%
AGAAGTTTAGCGGGAAACAT+TGG - Chr5:105044860-105044879 MsG0580030009.01.T01:CDS 40.0%
AGACTGTTTGGGAGAACTTA+TGG - Chr5:105045287-105045306 MsG0580030009.01.T01:intron 40.0%
AGTCTAGATACCCCATGAAT+TGG + Chr5:105046115-105046134 None:intergenic 40.0%
ATGATGTCATAGGCCAAACT+TGG + Chr5:105045854-105045873 None:intergenic 40.0%
CAAAATTTGTGCCGTAGGAA+GGG + Chr5:105044823-105044842 None:intergenic 40.0%
CAGAGGCTTCATTATAGTTG+AGG - Chr5:105046637-105046656 MsG0580030009.01.T01:intron 40.0%
CATTCCTAACACATCATGCA+GGG - Chr5:105045559-105045578 MsG0580030009.01.T01:intron 40.0%
CCCAATTGGTTGCATTGTAA+TGG - Chr5:105045066-105045085 MsG0580030009.01.T01:intron 40.0%
CCCATTACAATGCAACCAAT+TGG + Chr5:105045070-105045089 None:intergenic 40.0%
CTAGGATGGATGATCATGAT+AGG - Chr5:105046813-105046832 MsG0580030009.01.T01:intron 40.0%
CTGGGGATTGAAAAAATGGA+AGG + Chr5:105044911-105044930 None:intergenic 40.0%
GAACATGGCAAGAAGTTTAG+CGG - Chr5:105044850-105044869 MsG0580030009.01.T01:CDS 40.0%
GCTGTCAATTACTTGCATCT+AGG - Chr5:105047156-105047175 MsG0580030009.01.T01:five_prime_UTR 40.0%
GGGATCGGTTACTTAAAGTA+CGG - Chr5:105046184-105046203 MsG0580030009.01.T01:intron 40.0%
GTAACCAAAATTTGTGCCGT+AGG + Chr5:105044828-105044847 None:intergenic 40.0%
GTACCTGGGGATTGAAAAAA+TGG + Chr5:105044915-105044934 None:intergenic 40.0%
GTTTATTAGGATACACCTGC+AGG - Chr5:105046287-105046306 MsG0580030009.01.T01:CDS 40.0%
TAACTTCACCTACAGCATAC+TGG + Chr5:105046248-105046267 None:intergenic 40.0%
TAATGTTACGGGGAGGAAAT+TGG - Chr5:105046710-105046729 MsG0580030009.01.T01:intron 40.0%
TCTGAACAAATGGAAGAGGT+TGG - Chr5:105047072-105047091 MsG0580030009.01.T01:CDS 40.0%
TTACAAAATATGGCCCCGTT+TGG + Chr5:105045389-105045408 None:intergenic 40.0%
TTCGGATTGAATGCTTCAAG+AGG - Chr5:105045504-105045523 MsG0580030009.01.T01:intron 40.0%
TTGGTTACATTCAATCCTGC+AGG + Chr5:105046305-105046324 None:intergenic 40.0%
! AGAGACTTTAGTTCGTGTGT+TGG - Chr5:105045527-105045546 MsG0580030009.01.T01:intron 40.0%
! GAGTATTGTAGCTTTGCTGA+TGG - Chr5:105045599-105045618 MsG0580030009.01.T01:intron 40.0%
! TTAGTTCGTGTGTTGGTGTT+AGG - Chr5:105045534-105045553 MsG0580030009.01.T01:intron 40.0%
!! AGGTTCTGAACAAATGGAAG+AGG - Chr5:105047068-105047087 MsG0580030009.01.T01:CDS 40.0%
!! GTTGCATTGTAATGGGTTGA+TGG - Chr5:105045074-105045093 MsG0580030009.01.T01:intron 40.0%
!! TTGTTTTATCTCACGGGTCA+GGG - Chr5:105046399-105046418 MsG0580030009.01.T01:CDS 40.0%
!!! CTTCCATTTTTTCAATCCCC+AGG - Chr5:105044909-105044928 MsG0580030009.01.T01:intron 40.0%
!!! TTTGTTTTATCTCACGGGTC+AGG - Chr5:105046398-105046417 MsG0580030009.01.T01:CDS 40.0%
AGAGAATGTTCTCGCTTCAG+AGG - Chr5:105046620-105046639 MsG0580030009.01.T01:intron 45.0%
AGCCATAAAGTAAGCTCCGA+TGG + Chr5:105046457-105046476 None:intergenic 45.0%
AGCCGATTGTTCAGCATCAT+TGG + Chr5:105047139-105047158 None:intergenic 45.0%
CAGGGTGAAAATTCCCCATT+TGG - Chr5:105045577-105045596 MsG0580030009.01.T01:intron 45.0%
CCAAAATTTGTGCCGTAGGA+AGG + Chr5:105044824-105044843 None:intergenic 45.0%
CCTACAGCATACTGGTACTT+TGG + Chr5:105046240-105046259 None:intergenic 45.0%
GGTTGGAGATTCTTGTGAAC+AGG - Chr5:105047089-105047108 MsG0580030009.01.T01:CDS 45.0%
TTCACCCTGCATGATGTGTT+AGG + Chr5:105045566-105045585 None:intergenic 45.0%
! CCAAAGTACCAGTATGCTGT+AGG - Chr5:105046237-105046256 MsG0580030009.01.T01:CDS 45.0%
! CCTTCCTACGGCACAAATTT+TGG - Chr5:105044821-105044840 MsG0580030009.01.T01:CDS 45.0%
! TGTTTTATCTCACGGGTCAG+GGG - Chr5:105046400-105046419 MsG0580030009.01.T01:CDS 45.0%
CGCGAGTTCAACGTCTAGTT+CGG + Chr5:105045902-105045921 None:intergenic 50.0%
CTTCTGGGTCATGGTTCAAC+TGG - Chr5:105045925-105045944 MsG0580030009.01.T01:intron 50.0%
GAGAGTAGAGACCCTTCCTA+CGG - Chr5:105044809-105044828 MsG0580030009.01.T01:CDS 50.0%
GTTGAACTCGCGAGACTTCT+GGG - Chr5:105045910-105045929 MsG0580030009.01.T01:intron 50.0%
TTTGATGCTTGGCACGGGAT+CGG - Chr5:105046169-105046188 MsG0580030009.01.T01:intron 50.0%
! AGGTCGGTTCAAGGTTCAAC+CGG - Chr5:105046000-105046019 MsG0580030009.01.T01:intron 50.0%
CGGGGAGGAAATTGGGAGAA+AGG - Chr5:105046718-105046737 MsG0580030009.01.T01:intron 55.0%
CGTTGAACTCGCGAGACTTC+TGG - Chr5:105045909-105045928 MsG0580030009.01.T01:intron 55.0%
! ACTGGCTTAAACCGCTCGAG+CGG - Chr5:105045943-105045962 MsG0580030009.01.T01:intron 55.0%
!! CGGTTTTATCTCCGCTCGAG+CGG + Chr5:105045957-105045976 None:intergenic 55.0%
CTCGCGAGACTTCTGGGTCA+TGG - Chr5:105045916-105045935 MsG0580030009.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 105044784 105047182 105044784 ID=MsG0580030009.01;Name=MsG0580030009.01
Chr5 mRNA 105044784 105047182 105044784 ID=MsG0580030009.01.T01;Parent=MsG0580030009.01;Name=MsG0580030009.01.T01;_AED=0.50;_eAED=0.50;_QI=64|1|0.66|1|1|1|3|0|147
Chr5 exon 105044784 105044896 105044784 ID=MsG0580030009.01.T01:exon:21905;Parent=MsG0580030009.01.T01
Chr5 exon 105046229 105046476 105046229 ID=MsG0580030009.01.T01:exon:21904;Parent=MsG0580030009.01.T01
Chr5 exon 105047036 105047182 105047036 ID=MsG0580030009.01.T01:exon:21903;Parent=MsG0580030009.01.T01
Chr5 five_prime_UTR 105047119 105047182 105047119 ID=MsG0580030009.01.T01:five_prime_utr;Parent=MsG0580030009.01.T01
Chr5 CDS 105047036 105047118 105047036 ID=MsG0580030009.01.T01:cds;Parent=MsG0580030009.01.T01
Chr5 CDS 105046229 105046476 105046229 ID=MsG0580030009.01.T01:cds;Parent=MsG0580030009.01.T01
Chr5 CDS 105044784 105044896 105044784 ID=MsG0580030009.01.T01:cds;Parent=MsG0580030009.01.T01
Gene Sequence

>MsG0580030009.01.T01

ATGAACATGGCAAGAAGTTTAGCGGGAAACATTGGCTTCAAAATCTCATCTCATCTTCAACCTTCCATTTTTTCAATCCCCAGGAACATTTGGAAGTTTCGGATTGAATGCTTCAAGAGGAGAGACTTTAGTTCGTGTGTTGGTGTTAGGTACATTCCTAACACATCATGCAGGGTGAAAATTCCCCATTTGGAGTATTGTAGCTTTGCTGATGGCAGTGTAATCAAGATAAATGATGGGAAAATTGGGCATGCAACAATTCCGCATTGTGAAACTTCTCCAAATCCAGATGAAGTTCATACTGAGTTACTCTTTGATGATAGTGAAGAAGGTTCTGAACAAATGGAAGAGGTTGGAGATTCTTGTGAACAGGATTTCATACGTGTTGACAAATTTACCAATGATGCTGAACAATCGGCTGTCAATTACTTGCATCTAGGTTAG
Protein sequence

>MsG0580030009.01.T01

MNMARSLAGNIGFKISSHLQPSIFSIPRNIWKFRIECFKRRDFSSCVGVRYIPNTSCRVKIPHLEYCSFADGSVIKINDGKIGHATIPHCETSPNPDEVHTELLFDDSEEGSEQMEEVGDSCEQDFIRVDKFTNDAEQSAVNYLHLG*