AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030101.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030101.01.T01 MTR_5g097620 98.649 370 5 0 1 370 73 442 0.0 748
MsG0580030101.01.T01 MTR_8g009880 85.366 369 50 3 1 369 36 400 0.0 649
MsG0580030101.01.T01 MTR_4g133620 34.332 367 218 7 2 360 41 392 6.86e-65 211
MsG0580030101.01.T01 MTR_2g026080 33.875 369 219 9 2 362 45 396 3.39e-60 199
MsG0580030101.01.T01 MTR_7g051910 30.749 374 236 5 1 370 40 394 1.75e-59 197
MsG0580030101.01.T01 MTR_5g093520 35.143 350 202 11 3 342 54 388 1.92e-54 185
MsG0580030101.01.T01 MTR_3g058510 30.226 354 223 8 2 348 42 378 4.17e-51 175
MsG0580030101.01.T01 MTR_8g028075 30.453 243 156 6 102 339 319 553 6.53e-29 118
MsG0580030101.01.T01 MTR_7g117375 33.649 211 130 5 143 351 184 386 6.02e-21 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030101.01.T01 AT2G04850 70.492 366 106 2 1 365 38 402 0.0 533
MsG0580030101.01.T01 AT3G25290 32.222 360 223 6 1 352 43 389 7.67e-68 219
MsG0580030101.01.T01 AT3G25290 32.222 360 223 6 1 352 43 389 7.67e-68 219
MsG0580030101.01.T01 AT5G47530 32.973 370 222 7 2 367 43 390 2.44e-67 218
MsG0580030101.01.T01 AT4G12980 32.687 361 220 6 1 352 44 390 1.87e-66 216
MsG0580030101.01.T01 AT4G17280 33.056 360 218 7 2 355 50 392 3.14e-59 197
MsG0580030101.01.T01 AT5G35735 34.118 340 201 9 12 348 55 374 4.49e-59 196
MsG0580030101.01.T01 AT3G59070 30.959 365 221 11 21 370 72 420 1.54e-47 168
MsG0580030101.01.T01 AT3G07570 28.803 309 199 9 33 340 79 367 1.67e-31 122
MsG0580030101.01.T01 AT3G61750 29.508 305 192 10 33 336 11 293 1.48e-28 114
MsG0580030101.01.T01 AT3G61750 29.508 305 192 10 33 336 88 370 8.00e-28 113
MsG0580030101.01.T01 AT5G48750 28.899 218 138 4 1 216 49 251 7.18e-25 102
MsG0580030101.01.T01 AT5G54830 28.042 189 113 6 161 343 670 841 3.27e-13 71.6

Find 109 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACTATTTGTCCTGTATT+TGG 0.114722 5:+106692281 None:intergenic
TTAGGGCTTTCTACTCTTAT+TGG 0.174160 5:-106691492 MsG0580030101.01.T01:CDS
ATTCCAAACAAGGTGGATTT+TGG 0.191207 5:+106692079 None:intergenic
CAAATGTTTGGTTTCATTCT+TGG 0.192480 5:-106691792 MsG0580030101.01.T01:CDS
CCAAATTGAGTTATTGTTTA+GGG 0.198532 5:-106691509 MsG0580030101.01.T01:CDS
CTAAACTTGTTCGTAGTATT+TGG 0.209570 5:+106691639 None:intergenic
CCTTCTGGTTGGGTTGGATT+TGG 0.240455 5:-106692362 MsG0580030101.01.T01:CDS
GGTACTTTCATTTCACCTTC+TGG 0.252833 5:-106692377 MsG0580030101.01.T01:CDS
ATGAGGAAAGAGGGACTTAT+TGG 0.256520 5:-106691405 MsG0580030101.01.T01:CDS
ACCTTAGAACTAATCTTCTT+TGG 0.258541 5:-106692398 MsG0580030101.01.T01:CDS
GGTTACTCTTGTGTGGTTCT+TGG 0.269844 5:-106691585 MsG0580030101.01.T01:CDS
CCCTAAACAATAACTCAATT+TGG 0.277592 5:+106691509 None:intergenic
ATTCTTGGAACCATTGGTTT+TGG 0.279995 5:-106691777 MsG0580030101.01.T01:CDS
TATTGGTGTTTCCATTGCTT+TGG 0.291938 5:-106691475 MsG0580030101.01.T01:CDS
TTGCTTTGGAGGTCAATTCT+TGG 0.293222 5:-106691461 MsG0580030101.01.T01:CDS
AGACTTTGGCATTGTTGTTT+AGG 0.306216 5:-106691662 MsG0580030101.01.T01:CDS
AAACCAAAATCCACCTTGTT+TGG 0.312634 5:-106692082 MsG0580030101.01.T01:CDS
GCCAAATTGAGTTATTGTTT+AGG 0.317349 5:-106691510 MsG0580030101.01.T01:CDS
GAACTATTTGTCCTGTATTT+GGG 0.317546 5:+106692282 None:intergenic
ACCAAAGAAGATTAGTTCTA+AGG 0.327439 5:+106692397 None:intergenic
CAAATACTACGAACAAGTTT+AGG 0.331418 5:-106691638 MsG0580030101.01.T01:CDS
CCTTGGAGCTTTGCAGACTT+TGG 0.336139 5:-106691676 MsG0580030101.01.T01:CDS
TGCTTTGGAGGTCAATTCTT+GGG 0.347626 5:-106691460 MsG0580030101.01.T01:CDS
TTTCATTTCACCTTCTGGTT+GGG 0.360777 5:-106692372 MsG0580030101.01.T01:CDS
CGAACAAGTTTAGGAAGTAT+TGG 0.369522 5:-106691629 MsG0580030101.01.T01:CDS
CACAAGAGTAACCAACAAAA+TGG 0.369888 5:+106691595 None:intergenic
CTTCGACACTTTCAAACATT+AGG 0.375405 5:-106691846 MsG0580030101.01.T01:CDS
ATTTGTCCTGTATTTGGGTC+TGG 0.375488 5:+106692287 None:intergenic
TCTTCCTCTGCAGCCATGTA+TGG 0.375881 5:-106692176 MsG0580030101.01.T01:CDS
CATGCAGGAATACAAATGTT+TGG 0.389583 5:-106691804 MsG0580030101.01.T01:CDS
GGCTTTGTGAATGTGTTTCA+AGG 0.390916 5:-106691564 MsG0580030101.01.T01:CDS
GGTTTCATTCTTGGAACCAT+TGG 0.393037 5:-106691783 MsG0580030101.01.T01:CDS
ATCCACCTTGTTTGGAATAG+AGG 0.394891 5:-106692074 MsG0580030101.01.T01:CDS
TGTTCTAAATGCAGTCTCTT+GGG 0.395962 5:-106691907 MsG0580030101.01.T01:CDS
ATTTCACCTTCTGGTTGGGT+TGG 0.397941 5:-106692368 MsG0580030101.01.T01:CDS
ATTCGACTCGAGTTTGAGTT+TGG 0.399789 5:+106692106 None:intergenic
GATAGATCAATTGATGTTGT+TGG 0.401706 5:+106692017 None:intergenic
TGGTGTTTCCATTGCTTTGG+AGG 0.402874 5:-106691472 MsG0580030101.01.T01:CDS
AGTTTGGCGTAGATTTGAAT+AGG 0.405047 5:+106692122 None:intergenic
AATAGAGGTCTTTATGTTCA+AGG 0.414486 5:-106692059 MsG0580030101.01.T01:CDS
TTGGTATTGGTATTCAGCTT+GGG 0.420179 5:-106691758 MsG0580030101.01.T01:CDS
GTGTTCTAAATGCAGTCTCT+TGG 0.422091 5:-106691908 MsG0580030101.01.T01:CDS
CTTTCATTTCACCTTCTGGT+TGG 0.424491 5:-106692373 MsG0580030101.01.T01:CDS
TTTCAAGGGTTTGAAGTGAT+TGG 0.434245 5:-106691549 MsG0580030101.01.T01:CDS
CCTGTCATTTGTGGTGAAGT+AGG 0.437815 5:+106692332 None:intergenic
TTTGGTATTGGTATTCAGCT+TGG 0.448672 5:-106691759 MsG0580030101.01.T01:CDS
AGCCATGTATGGTGGCAAAA+TGG 0.449205 5:-106692165 MsG0580030101.01.T01:CDS
GAAGAGAAAATGAGGAAAGA+GGG 0.453995 5:-106691414 MsG0580030101.01.T01:CDS
GCATTAGTTCCTGTCATTTG+TGG 0.456046 5:+106692323 None:intergenic
TACGGACTCCACAGGAAGCT+TGG 0.471361 5:-106691714 MsG0580030101.01.T01:CDS
CTGTCATTTGTGGTGAAGTA+GGG 0.474757 5:+106692333 None:intergenic
AATAGGCGCACCGTTATGAA+TGG 0.475616 5:+106692139 None:intergenic
TTGAAGTGATTGGTGCTAGT+AGG 0.475727 5:-106691539 MsG0580030101.01.T01:CDS
ACAATGTTAAGAGTGATTCA+TGG 0.476434 5:-106691930 MsG0580030101.01.T01:CDS
AGGAGGTTGATATTATCAAA+AGG 0.484868 5:+106692200 None:intergenic
GGAAGAGAAAATGAGGAAAG+AGG 0.485997 5:-106691415 MsG0580030101.01.T01:CDS
AATTGATGTTGTTGGATGGA+TGG 0.487039 5:+106692025 None:intergenic
TGAATCACTCTTAACATTGT+GGG 0.490877 5:+106691933 None:intergenic
TGGGAAAATGACGCCGGGAG+TGG 0.491162 5:-106691739 MsG0580030101.01.T01:CDS
AATCTAAGGAAGAGAAAATG+AGG 0.507309 5:-106691422 MsG0580030101.01.T01:CDS
TGCTATTGAGGCTTGTTGTG+TGG 0.507444 5:+106692445 None:intergenic
GCATGAGCATAAAACCATGC+AGG 0.507446 5:+106691822 None:intergenic
TTGAGGCTTGTTGTGTGGGA+AGG 0.508773 5:+106692450 None:intergenic
TCATTTCCAGACCCAAATAC+AGG 0.515763 5:-106692293 MsG0580030101.01.T01:CDS
TTGGAACTAGTTCTGACAAA+GGG 0.523954 5:-106691386 MsG0580030101.01.T01:CDS
ACCTGACAAAACATCAAAAG+TGG 0.524778 5:+106691986 None:intergenic
GCTATTGAGGCTTGTTGTGT+GGG 0.533375 5:+106692446 None:intergenic
GTTCTAAATGCAGTCTCTTG+GGG 0.536425 5:-106691906 MsG0580030101.01.T01:CDS
GATGTTGTTGGATGGATGGT+TGG 0.537684 5:+106692029 None:intergenic
ATGAATCACTCTTAACATTG+TGG 0.537907 5:+106691932 None:intergenic
GATCAATTGATGTTGTTGGA+TGG 0.539796 5:+106692021 None:intergenic
GCTTTGTGAATGTGTTTCAA+GGG 0.546917 5:-106691563 MsG0580030101.01.T01:CDS
ACAGTAGTGTCAAGAATGAA+AGG 0.550198 5:+106692254 None:intergenic
TGGGTCTGGAAATGATATCA+AGG 0.553986 5:+106692301 None:intergenic
ATGACGCCGGGAGTGGAGTA+CGG 0.557724 5:-106691732 MsG0580030101.01.T01:CDS
GTTCTAAGGTGGAGCTGTGA+GGG 0.559595 5:+106692411 None:intergenic
CACAACAAGCCTCAATAGCA+TGG 0.563316 5:-106692442 MsG0580030101.01.T01:CDS
TTCAAACATTAGGCCCTGCA+TGG 0.563413 5:-106691836 MsG0580030101.01.T01:CDS
TCCTCTGCAGCCATGTATGG+TGG 0.565035 5:-106692173 MsG0580030101.01.T01:CDS
GCCACCATACATGGCTGCAG+AGG 0.571339 5:+106692172 None:intergenic
CAGCTTGGGAAAATGACGCC+GGG 0.580014 5:-106691744 MsG0580030101.01.T01:CDS
AGAATTGACCTCCAAAGCAA+TGG 0.580565 5:+106691464 None:intergenic
AAAGAAGATTAGTTCTAAGG+TGG 0.580824 5:+106692400 None:intergenic
TGGAGTCCGTACTCCACTCC+CGG 0.581144 5:+106691726 None:intergenic
ACATGGCTGCAGAGGAAGAG+AGG 0.585108 5:+106692180 None:intergenic
GTGGAAAGTCCATGCTATTG+AGG 0.587691 5:+106692433 None:intergenic
CATTGTTGCTCTCTCAAATG+TGG 0.591191 5:+106691352 None:intergenic
GAGTGGAGTACGGACTCCAC+AGG 0.594153 5:-106691722 MsG0580030101.01.T01:CDS
AATACCAATACCAAAACCAA+TGG 0.596565 5:+106691767 None:intergenic
CCAAAGTCTGCAAAGCTCCA+AGG 0.604381 5:+106691676 None:intergenic
GTCAGTGTGTTGAGATGATG+AGG 0.606185 5:+106691956 None:intergenic
CCGCTATTCCAAGCTTCCTG+TGG 0.608097 5:+106691706 None:intergenic
CATGAGCATAAAACCATGCA+GGG 0.608239 5:+106691823 None:intergenic
CCACAGGAAGCTTGGAATAG+CGG 0.609878 5:-106691706 MsG0580030101.01.T01:CDS
TCAGCTTGGGAAAATGACGC+CGG 0.614573 5:-106691745 MsG0580030101.01.T01:CDS
TCAAAAGGTTGAGAGAGAAG+TGG 0.614618 5:+106692215 None:intergenic
AAAATGGCAACCATTCATAA+CGG 0.616375 5:-106692149 MsG0580030101.01.T01:CDS
CCAAATCCAACCCAACCAGA+AGG 0.618606 5:+106692362 None:intergenic
CCTACTTCACCACAAATGAC+AGG 0.618913 5:-106692332 MsG0580030101.01.T01:CDS
AGTTCTGACAAAGGGAGTAG+TGG 0.620980 5:-106691378 MsG0580030101.01.T01:CDS
CTAAGGTGGAGCTGTGAGGG+TGG 0.630452 5:+106692414 None:intergenic
AGTTCTAAGGTGGAGCTGTG+AGG 0.642313 5:+106692410 None:intergenic
GAATCACTCTTAACATTGTG+GGG 0.643050 5:+106691934 None:intergenic
ATTGGAACTAGTTCTGACAA+AGG 0.648605 5:-106691387 MsG0580030101.01.T01:CDS
AAAGACCTCTATTCCAAACA+AGG 0.655433 5:+106692069 None:intergenic
CGTGCTGTTATCGCTCCCGT+AGG 0.660166 5:+106691873 None:intergenic
GACCTCTATTCCAAACAAGG+TGG 0.670337 5:+106692072 None:intergenic
AAGAGTAACCAACAAAATGG+TGG 0.683274 5:+106691598 None:intergenic
TGGCTGCAGAGGAAGAGAGG+AGG 0.691481 5:+106692183 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTGTAGGAAATCTA+AGG - Chr5:106692384-106692403 MsG0580030101.01.T01:CDS 20.0%
! CCAAATTGAGTTATTGTTTA+GGG - Chr5:106692311-106692330 MsG0580030101.01.T01:CDS 25.0%
!!! ATTCTTGGGTTATTTTTTGT+AGG - Chr5:106692374-106692393 MsG0580030101.01.T01:CDS 25.0%
AAAATGGCAACCATTCATAA+CGG - Chr5:106691671-106691690 MsG0580030101.01.T01:CDS 30.0%
AATACCAATACCAAAACCAA+TGG + Chr5:106692056-106692075 None:intergenic 30.0%
AATAGAGGTCTTTATGTTCA+AGG - Chr5:106691761-106691780 MsG0580030101.01.T01:CDS 30.0%
AATCTAAGGAAGAGAAAATG+AGG - Chr5:106692398-106692417 MsG0580030101.01.T01:CDS 30.0%
ACAATGTTAAGAGTGATTCA+TGG - Chr5:106691890-106691909 MsG0580030101.01.T01:CDS 30.0%
ACCTTAGAACTAATCTTCTT+TGG - Chr5:106691422-106691441 MsG0580030101.01.T01:CDS 30.0%
AGAACTATTTGTCCTGTATT+TGG + Chr5:106691542-106691561 None:intergenic 30.0%
ATGAATCACTCTTAACATTG+TGG + Chr5:106691891-106691910 None:intergenic 30.0%
CAAATACTACGAACAAGTTT+AGG - Chr5:106692182-106692201 MsG0580030101.01.T01:CDS 30.0%
CCCTAAACAATAACTCAATT+TGG + Chr5:106692314-106692333 None:intergenic 30.0%
GAACTATTTGTCCTGTATTT+GGG + Chr5:106691541-106691560 None:intergenic 30.0%
GATAGATCAATTGATGTTGT+TGG + Chr5:106691806-106691825 None:intergenic 30.0%
GCCAAATTGAGTTATTGTTT+AGG - Chr5:106692310-106692329 MsG0580030101.01.T01:CDS 30.0%
TGAATCACTCTTAACATTGT+GGG + Chr5:106691890-106691909 None:intergenic 30.0%
! AAATCTTACCACCATTTTGT+TGG - Chr5:106692214-106692233 MsG0580030101.01.T01:CDS 30.0%
! CAAATGTTTGGTTTCATTCT+TGG - Chr5:106692028-106692047 MsG0580030101.01.T01:CDS 30.0%
! CTAAACTTGTTCGTAGTATT+TGG + Chr5:106692184-106692203 None:intergenic 30.0%
!! AAAGAAGATTAGTTCTAAGG+TGG + Chr5:106691423-106691442 None:intergenic 30.0%
!! ACCAAAGAAGATTAGTTCTA+AGG + Chr5:106691426-106691445 None:intergenic 30.0%
!! AGGAGGTTGATATTATCAAA+AGG + Chr5:106691623-106691642 None:intergenic 30.0%
AAACCAAAATCCACCTTGTT+TGG - Chr5:106691738-106691757 MsG0580030101.01.T01:CDS 35.0%
AAAGACCTCTATTCCAAACA+AGG + Chr5:106691754-106691773 None:intergenic 35.0%
AAGAGTAACCAACAAAATGG+TGG + Chr5:106692225-106692244 None:intergenic 35.0%
AATTGATGTTGTTGGATGGA+TGG + Chr5:106691798-106691817 None:intergenic 35.0%
ACAGTAGTGTCAAGAATGAA+AGG + Chr5:106691569-106691588 None:intergenic 35.0%
ACCTGACAAAACATCAAAAG+TGG + Chr5:106691837-106691856 None:intergenic 35.0%
AGTTTGGCGTAGATTTGAAT+AGG + Chr5:106691701-106691720 None:intergenic 35.0%
CACAAGAGTAACCAACAAAA+TGG + Chr5:106692228-106692247 None:intergenic 35.0%
CATGCAGGAATACAAATGTT+TGG - Chr5:106692016-106692035 MsG0580030101.01.T01:CDS 35.0%
CTTCGACACTTTCAAACATT+AGG - Chr5:106691974-106691993 MsG0580030101.01.T01:CDS 35.0%
GAAGAGAAAATGAGGAAAGA+GGG - Chr5:106692406-106692425 MsG0580030101.01.T01:CDS 35.0%
GAATCACTCTTAACATTGTG+GGG + Chr5:106691889-106691908 None:intergenic 35.0%
GATCAATTGATGTTGTTGGA+TGG + Chr5:106691802-106691821 None:intergenic 35.0%
GCTTTGTGAATGTGTTTCAA+GGG - Chr5:106692257-106692276 MsG0580030101.01.T01:CDS 35.0%
TTAGGGCTTTCTACTCTTAT+TGG - Chr5:106692328-106692347 MsG0580030101.01.T01:CDS 35.0%
! CGAACAAGTTTAGGAAGTAT+TGG - Chr5:106692191-106692210 MsG0580030101.01.T01:CDS 35.0%
! TATTGGTGTTTCCATTGCTT+TGG - Chr5:106692345-106692364 MsG0580030101.01.T01:CDS 35.0%
! TGTTCTAAATGCAGTCTCTT+GGG - Chr5:106691913-106691932 MsG0580030101.01.T01:CDS 35.0%
! TTGGGGTATACTTTTACCTA+CGG - Chr5:106691931-106691950 MsG0580030101.01.T01:CDS 35.0%
! TTTCAAGGGTTTGAAGTGAT+TGG - Chr5:106692271-106692290 MsG0580030101.01.T01:CDS 35.0%
! TTTCATTTCACCTTCTGGTT+GGG - Chr5:106691448-106691467 MsG0580030101.01.T01:CDS 35.0%
!! AGACTTTGGCATTGTTGTTT+AGG - Chr5:106692158-106692177 MsG0580030101.01.T01:CDS 35.0%
!! ATTCCAAACAAGGTGGATTT+TGG + Chr5:106691744-106691763 None:intergenic 35.0%
!! ATTGGAACTAGTTCTGACAA+AGG - Chr5:106692433-106692452 MsG0580030101.01.T01:CDS 35.0%
!! TTGGAACTAGTTCTGACAAA+GGG - Chr5:106692434-106692453 MsG0580030101.01.T01:CDS 35.0%
!! TTGGTATTGGTATTCAGCTT+GGG - Chr5:106692062-106692081 MsG0580030101.01.T01:CDS 35.0%
!! TTTGGTATTGGTATTCAGCT+TGG - Chr5:106692061-106692080 MsG0580030101.01.T01:CDS 35.0%
!! TTTTGTTGGTTACTCTTGTG+TGG - Chr5:106692228-106692247 MsG0580030101.01.T01:CDS 35.0%
!!! ATTCTTGGAACCATTGGTTT+TGG - Chr5:106692043-106692062 MsG0580030101.01.T01:CDS 35.0%
AGAATTGACCTCCAAAGCAA+TGG + Chr5:106692359-106692378 None:intergenic 40.0%
ATGAGGAAAGAGGGACTTAT+TGG - Chr5:106692415-106692434 MsG0580030101.01.T01:CDS 40.0%
ATTTGTCCTGTATTTGGGTC+TGG + Chr5:106691536-106691555 None:intergenic 40.0%
CATGAGCATAAAACCATGCA+GGG + Chr5:106692000-106692019 None:intergenic 40.0%
CTTTCATTTCACCTTCTGGT+TGG - Chr5:106691447-106691466 MsG0580030101.01.T01:CDS 40.0%
GGAAGAGAAAATGAGGAAAG+AGG - Chr5:106692405-106692424 MsG0580030101.01.T01:CDS 40.0%
GGTACTTTCATTTCACCTTC+TGG - Chr5:106691443-106691462 MsG0580030101.01.T01:CDS 40.0%
GGTTTCATTCTTGGAACCAT+TGG - Chr5:106692037-106692056 MsG0580030101.01.T01:CDS 40.0%
TCAAAAGGTTGAGAGAGAAG+TGG + Chr5:106691608-106691627 None:intergenic 40.0%
TCATTTCCAGACCCAAATAC+AGG - Chr5:106691527-106691546 MsG0580030101.01.T01:CDS 40.0%
TGGGTCTGGAAATGATATCA+AGG + Chr5:106691522-106691541 None:intergenic 40.0%
! ATCCACCTTGTTTGGAATAG+AGG - Chr5:106691746-106691765 MsG0580030101.01.T01:CDS 40.0%
! ATTCGACTCGAGTTTGAGTT+TGG + Chr5:106691717-106691736 None:intergenic 40.0%
! CTGTCATTTGTGGTGAAGTA+GGG + Chr5:106691490-106691509 None:intergenic 40.0%
! GAGAGAGAAGTGGTGATTTT+TGG + Chr5:106691598-106691617 None:intergenic 40.0%
! GCATTAGTTCCTGTCATTTG+TGG + Chr5:106691500-106691519 None:intergenic 40.0%
! GGCTTTGTGAATGTGTTTCA+AGG - Chr5:106692256-106692275 MsG0580030101.01.T01:CDS 40.0%
! GTGTTCTAAATGCAGTCTCT+TGG - Chr5:106691912-106691931 MsG0580030101.01.T01:CDS 40.0%
! GTTCTAAATGCAGTCTCTTG+GGG - Chr5:106691914-106691933 MsG0580030101.01.T01:CDS 40.0%
! TGGGGTATACTTTTACCTAC+GGG - Chr5:106691932-106691951 MsG0580030101.01.T01:CDS 40.0%
!! TGCTTTGGAGGTCAATTCTT+GGG - Chr5:106692360-106692379 MsG0580030101.01.T01:CDS 40.0%
!! TTGAAGTGATTGGTGCTAGT+AGG - Chr5:106692281-106692300 MsG0580030101.01.T01:CDS 40.0%
!! TTGCTTTGGAGGTCAATTCT+TGG - Chr5:106692359-106692378 MsG0580030101.01.T01:CDS 40.0%
!!! GCCACTTTTGATGTTTTGTC+AGG - Chr5:106691833-106691852 MsG0580030101.01.T01:CDS 40.0%
!!! GGAACCATTGGTTTTGGTAT+TGG - Chr5:106692049-106692068 MsG0580030101.01.T01:CDS 40.0%
AATAGGCGCACCGTTATGAA+TGG + Chr5:106691684-106691703 None:intergenic 45.0%
AGCCATGTATGGTGGCAAAA+TGG - Chr5:106691655-106691674 MsG0580030101.01.T01:CDS 45.0%
CACAACAAGCCTCAATAGCA+TGG - Chr5:106691378-106691397 MsG0580030101.01.T01:CDS 45.0%
CCTACTTCACCACAAATGAC+AGG - Chr5:106691488-106691507 MsG0580030101.01.T01:CDS 45.0%
GACCTCTATTCCAAACAAGG+TGG + Chr5:106691751-106691770 None:intergenic 45.0%
GATGTTGTTGGATGGATGGT+TGG + Chr5:106691794-106691813 None:intergenic 45.0%
GCATGAGCATAAAACCATGC+AGG + Chr5:106692001-106692020 None:intergenic 45.0%
GGTTACTCTTGTGTGGTTCT+TGG - Chr5:106692235-106692254 MsG0580030101.01.T01:CDS 45.0%
GTCAGTGTGTTGAGATGATG+AGG + Chr5:106691867-106691886 None:intergenic 45.0%
GTGGAAAGTCCATGCTATTG+AGG + Chr5:106691390-106691409 None:intergenic 45.0%
TGGTGTTTCCATTGCTTTGG+AGG - Chr5:106692348-106692367 MsG0580030101.01.T01:CDS 45.0%
TTCAAACATTAGGCCCTGCA+TGG - Chr5:106691984-106692003 MsG0580030101.01.T01:CDS 45.0%
! AGTTCTGACAAAGGGAGTAG+TGG - Chr5:106692442-106692461 MsG0580030101.01.T01:CDS 45.0%
! ATTTCACCTTCTGGTTGGGT+TGG - Chr5:106691452-106691471 MsG0580030101.01.T01:CDS 45.0%
! CCTGTCATTTGTGGTGAAGT+AGG + Chr5:106691491-106691510 None:intergenic 45.0%
!! GCTATTGAGGCTTGTTGTGT+GGG + Chr5:106691377-106691396 None:intergenic 45.0%
!! TGCTATTGAGGCTTGTTGTG+TGG + Chr5:106691378-106691397 None:intergenic 45.0%
!!! GCATGGTTTTATGCTCATGC+AGG - Chr5:106692001-106692020 MsG0580030101.01.T01:CDS 45.0%
!!! TGCCATTTTGCCACCATACA+TGG + Chr5:106691660-106691679 None:intergenic 45.0%
CCAAAGTCTGCAAAGCTCCA+AGG + Chr5:106692147-106692166 None:intergenic 50.0%
CCAAATCCAACCCAACCAGA+AGG + Chr5:106691461-106691480 None:intergenic 50.0%
CCACAGGAAGCTTGGAATAG+CGG - Chr5:106692114-106692133 MsG0580030101.01.T01:CDS 50.0%
CCTTGGAGCTTTGCAGACTT+TGG - Chr5:106692144-106692163 MsG0580030101.01.T01:CDS 50.0%
TCAGCTTGGGAAAATGACGC+CGG - Chr5:106692075-106692094 MsG0580030101.01.T01:CDS 50.0%
TCTTCCTCTGCAGCCATGTA+TGG - Chr5:106691644-106691663 MsG0580030101.01.T01:CDS 50.0%
! GGAATAGCGGTGTTTTGCCT+TGG - Chr5:106692127-106692146 MsG0580030101.01.T01:CDS 50.0%
!! AGTTCTAAGGTGGAGCTGTG+AGG + Chr5:106691413-106691432 None:intergenic 50.0%
!! CCTTCTGGTTGGGTTGGATT+TGG - Chr5:106691458-106691477 MsG0580030101.01.T01:CDS 50.0%
!! GTTCTAAGGTGGAGCTGTGA+GGG + Chr5:106691412-106691431 None:intergenic 50.0%
!! TTGAGGCTTGTTGTGTGGGA+AGG + Chr5:106691373-106691392 None:intergenic 50.0%
ACATGGCTGCAGAGGAAGAG+AGG + Chr5:106691643-106691662 None:intergenic 55.0%
CAGCTTGGGAAAATGACGCC+GGG - Chr5:106692076-106692095 MsG0580030101.01.T01:CDS 55.0%
CCGCTATTCCAAGCTTCCTG+TGG + Chr5:106692117-106692136 None:intergenic 55.0%
TACGGACTCCACAGGAAGCT+TGG - Chr5:106692106-106692125 MsG0580030101.01.T01:CDS 55.0%
TCCTCTGCAGCCATGTATGG+TGG - Chr5:106691647-106691666 MsG0580030101.01.T01:CDS 55.0%
ATGACGCCGGGAGTGGAGTA+CGG - Chr5:106692088-106692107 MsG0580030101.01.T01:CDS 60.0%
CTAAGGTGGAGCTGTGAGGG+TGG + Chr5:106691409-106691428 None:intergenic 60.0%
GAGTGGAGTACGGACTCCAC+AGG - Chr5:106692098-106692117 MsG0580030101.01.T01:CDS 60.0%
GCCACCATACATGGCTGCAG+AGG + Chr5:106691651-106691670 None:intergenic 60.0%
TGGAGTCCGTACTCCACTCC+CGG + Chr5:106692097-106692116 None:intergenic 60.0%
TGGCTGCAGAGGAAGAGAGG+AGG + Chr5:106691640-106691659 None:intergenic 60.0%
TGGGAAAATGACGCCGGGAG+TGG - Chr5:106692081-106692100 MsG0580030101.01.T01:CDS 60.0%
! CGTGCTGTTATCGCTCCCGT+AGG + Chr5:106691950-106691969 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 106691365 106692477 106691365 ID=MsG0580030101.01;Name=MsG0580030101.01
Chr5 mRNA 106691365 106692477 106691365 ID=MsG0580030101.01.T01;Parent=MsG0580030101.01;Name=MsG0580030101.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|370
Chr5 exon 106691365 106692477 106691365 ID=MsG0580030101.01.T01:exon:17254;Parent=MsG0580030101.01.T01
Chr5 CDS 106691365 106692477 106691365 ID=MsG0580030101.01.T01:cds;Parent=MsG0580030101.01.T01
Gene Sequence

>MsG0580030101.01.T01

ATGAACCTTCCCACACAACAAGCCTCAATAGCATGGACTTTCCACCCTCACAGCTCCACCTTAGAACTAATCTTCTTTGGTACTTTCATTTCACCTTCTGGTTGGGTTGGATTTGGCATCAACCCTACTTCACCACAAATGACAGGAACTAATGCCTTGATATCATTTCCAGACCCAAATACAGGACAAATAGTTCTTCTACCTTTCATTCTTGACACTACTGTAAAACTCCAAAAATCACCACTTCTCTCTCAACCTTTTGATAATATCAACCTCCTCTCTTCCTCTGCAGCCATGTATGGTGGCAAAATGGCAACCATTCATAACGGTGCGCCTATTCAAATCTACGCCAAACTCAAACTCGAGTCGAATAAAACCAAAATCCACCTTGTTTGGAATAGAGGTCTTTATGTTCAAGGCTACTCACCAACCATCCATCCAACAACATCAATTGATCTATCTTCTATTGCCACTTTTGATGTTTTGTCAGGTTCATCATCCTCATCATCTCAACACACTGACCCCACAATGTTAAGAGTGATTCATGGTGTTCTAAATGCAGTCTCTTGGGGTATACTTTTACCTACGGGAGCGATAACAGCACGTTATCTTCGACACTTTCAAACATTAGGCCCTGCATGGTTTTATGCTCATGCAGGAATACAAATGTTTGGTTTCATTCTTGGAACCATTGGTTTTGGTATTGGTATTCAGCTTGGGAAAATGACGCCGGGAGTGGAGTACGGACTCCACAGGAAGCTTGGAATAGCGGTGTTTTGCCTTGGAGCTTTGCAGACTTTGGCATTGTTGTTTAGGCCAAATACTACGAACAAGTTTAGGAAGTATTGGAAATCTTACCACCATTTTGTTGGTTACTCTTGTGTGGTTCTTGGCTTTGTGAATGTGTTTCAAGGGTTTGAAGTGATTGGTGCTAGTAGGTCTTATGCCAAATTGAGTTATTGTTTAGGGCTTTCTACTCTTATTGGTGTTTCCATTGCTTTGGAGGTCAATTCTTGGGTTATTTTTTGTAGGAAATCTAAGGAAGAGAAAATGAGGAAAGAGGGACTTATTGGAACTAGTTCTGACAAAGGGAGTAGTGGCATCCACATTTGA
Protein sequence

>MsG0580030101.01.T01

MNLPTQQASIAWTFHPHSSTLELIFFGTFISPSGWVGFGINPTSPQMTGTNALISFPDPNTGQIVLLPFILDTTVKLQKSPLLSQPFDNINLLSSSAAMYGGKMATIHNGAPIQIYAKLKLESNKTKIHLVWNRGLYVQGYSPTIHPTTSIDLSSIATFDVLSGSSSSSSQHTDPTMLRVIHGVLNAVSWGILLPTGAITARYLRHFQTLGPAWFYAHAGIQMFGFILGTIGFGIGIQLGKMTPGVEYGLHRKLGIAVFCLGALQTLALLFRPNTTNKFRKYWKSYHHFVGYSCVVLGFVNVFQGFEVIGASRSYAKLSYCLGLSTLIGVSIALEVNSWVIFCRKSKEEKMRKEGLIGTSSDKGSSGIHI*