Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030107.01.T01 | XP_024632833.1 | 42.126 | 254 | 138 | 5 | 7 | 256 | 9 | 257 | 6.62E-61 | 203 |
| MsG0580030107.01.T02 | RHN55246.1 | 63.049 | 387 | 108 | 9 | 9 | 366 | 7 | 387 | 3.33E-156 | 454 |
| MsG0580030107.01.T03 | XP_024640535.1 | 61.081 | 185 | 48 | 4 | 4 | 164 | 157 | 341 | 3.43E-67 | 218 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030107.01.T01 | G7K4W8 | 72.535 | 142 | 31 | 2 | 2 | 143 | 14 | 147 | 4.93e-59 | 194 |
| MsG0580030107.01.T02 | A0A396HPH8 | 63.049 | 387 | 108 | 9 | 9 | 366 | 7 | 387 | 1.59e-156 | 454 |
| MsG0580030107.01.T03 | A0A396HPH8 | 61.081 | 185 | 48 | 4 | 4 | 164 | 203 | 387 | 5.07e-67 | 218 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030107.01.T01 | MTR_5g097650 | 72.535 | 142 | 31 | 2 | 2 | 143 | 14 | 147 | 1.25e-62 | 194 |
| MsG0580030107.01.T01 | MTR_5g097820 | 59.211 | 152 | 51 | 3 | 3 | 143 | 16 | 167 | 5.64e-52 | 170 |
| MsG0580030107.01.T01 | MTR_5g037680 | 61.842 | 152 | 43 | 3 | 7 | 143 | 5 | 156 | 2.67e-51 | 174 |
| MsG0580030107.01.T01 | MTR_5g037680 | 68.807 | 109 | 30 | 1 | 146 | 250 | 272 | 380 | 1.52e-45 | 158 |
| MsG0580030107.01.T01 | MTR_6g090260 | 38.433 | 268 | 125 | 7 | 5 | 253 | 15 | 261 | 7.28e-50 | 164 |
| MsG0580030107.01.T01 | MTR_5g059430 | 70.642 | 109 | 31 | 1 | 150 | 257 | 319 | 427 | 4.64e-47 | 162 |
| MsG0580030107.01.T01 | MTR_5g059430 | 51.366 | 183 | 60 | 5 | 3 | 157 | 18 | 199 | 6.79e-47 | 161 |
| MsG0580030107.01.T01 | MTR_5g069390 | 56.329 | 158 | 52 | 3 | 3 | 143 | 18 | 175 | 1.00e-45 | 160 |
| MsG0580030107.01.T01 | MTR_5g069390 | 70.755 | 106 | 31 | 0 | 151 | 256 | 312 | 417 | 1.23e-44 | 157 |
| MsG0580030107.01.T01 | MTR_5g097660 | 65.179 | 112 | 38 | 1 | 147 | 257 | 10 | 121 | 1.55e-45 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030107.01.T01 | AT3G16210 | 34.058 | 138 | 75 | 5 | 7 | 144 | 2 | 123 | 2.08e-12 | 66.6 |
| MsG0580030107.01.T01 | AT1G47790 | 29.054 | 148 | 92 | 4 | 13 | 157 | 28 | 165 | 1.11e-11 | 64.7 |
| MsG0580030107.01.T02 | AT3G16210 | 27.815 | 302 | 170 | 13 | 7 | 297 | 2 | 266 | 5.05e-21 | 93.6 |
| MsG0580030107.01.T02 | AT4G12560 | 23.773 | 387 | 220 | 16 | 13 | 353 | 7 | 364 | 1.15e-16 | 81.3 |
| MsG0580030107.01.T02 | AT4G12560 | 23.773 | 387 | 220 | 16 | 13 | 353 | 7 | 364 | 1.15e-16 | 81.3 |
| MsG0580030107.01.T02 | AT4G12560 | 23.773 | 387 | 220 | 16 | 13 | 353 | 7 | 364 | 1.15e-16 | 81.3 |
| MsG0580030107.01.T02 | AT3G17530 | 24.514 | 257 | 151 | 10 | 13 | 251 | 9 | 240 | 6.43e-12 | 66.6 |
| MsG0580030107.01.T02 | AT1G47790 | 29.609 | 179 | 98 | 7 | 13 | 186 | 28 | 183 | 1.33e-11 | 65.9 |
Find 68 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGATGGTCCTTGGGA+TGG | 0.530576 | 5:-106756611 | MsG0580030107.01.T02:CDS |
| AAAGTCCATCCGTGTACAGT+TGG | 0.577168 | 5:+106756183 | None:intergenic |
| AAAGTGATGCGTCTTGGTGT+AGG | 0.508647 | 5:-106756296 | MsG0580030107.01.T02:CDS |
| AACCGATACGTAACAACTAT+TGG | 0.427509 | 5:-106756214 | MsG0580030107.01.T02:CDS |
| AACTCGGTGTAATGGAATCA+TGG | 0.517397 | 5:-106755965 | MsG0580030107.01.T02:CDS |
| AAGAGTTCCATGAACACTTC+TGG | 0.346665 | 5:+106756454 | None:intergenic |
| AAGTGATGCGTCTTGGTGTA+GGG | 0.514281 | 5:-106756295 | MsG0580030107.01.T02:CDS |
| AATCGTTTGAAAGATTTAAT+AGG | 0.281695 | 5:+106756758 | None:intergenic |
| AATCTGAGATATATAGTCTA+AGG | 0.422967 | 5:-106756271 | MsG0580030107.01.T02:CDS |
| ACACGGATGGACTTTCTCAC+TGG | 0.548528 | 5:-106756175 | MsG0580030107.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATCTTAAATCATACAGACA+CGG | - | Chr5:106756555-106756574 | MsG0580030107.01.T02:CDS | 30.0% | |
| GGACTAAAATCTTCATTGTA+GGG | - | Chr5:106756626-106756645 | MsG0580030107.01.T02:CDS | 30.0% | |
| TGGACTAAAATCTTCATTGT+AGG | - | Chr5:106756625-106756644 | MsG0580030107.01.T02:CDS | 30.0% | |
| TGGTAAAAGGTTTGAGAATA+TGG | - | Chr5:106756021-106756040 | MsG0580030107.01.T02:CDS | 30.0% | |
| TTTAAATTCCCAAACGATTG+AGG | - | Chr5:106756738-106756757 | MsG0580030107.01.T02:CDS | 30.0% | |
| AACCGATACGTAACAACTAT+TGG | - | Chr5:106756356-106756375 | MsG0580030107.01.T02:CDS | 35.0% | |
| AGATTCAGTGTGGAAACATT+TGG | - | Chr5:106756507-106756526 | MsG0580030107.01.T02:CDS | 35.0% | |
| ATTGTGGAATCCATCTACTA+AGG | - | Chr5:106756171-106756190 | MsG0580030107.01.T02:CDS | 35.0% | |
| GAAAGTCTTCTTCCATTTGA+AGG | - | Chr5:106756811-106756830 | MsG0580030107.01.T02:CDS | 35.0% | |
| GAATTCAAAATCATTCCTCC+TGG | - | Chr5:106756193-106756212 | MsG0580030107.01.T02:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 106755746 | 106756846 | 106755746 | ID=MsG0580030107.01;Name=MsG0580030107.01 |
| Chr5 | mRNA | 106755746 | 106756846 | 106755746 | ID=MsG0580030107.01.T01;Parent=MsG0580030107.01;Name=MsG0580030107.01.T01;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|0|0|3|0|257 |
| Chr5 | exon | 106755746 | 106756089 | 106755746 | ID=MsG0580030107.01.T01:exon:17264;Parent=MsG0580030107.01.T01 |
| Chr5 | exon | 106756381 | 106756575 | 106756381 | ID=MsG0580030107.01.T01:exon:17263;Parent=MsG0580030107.01.T01 |
| Chr5 | exon | 106756612 | 106756846 | 106756612 | ID=MsG0580030107.01.T01:exon:17262;Parent=MsG0580030107.01.T01 |
| Chr5 | CDS | 106756612 | 106756846 | 106756612 | ID=MsG0580030107.01.T01:cds;Parent=MsG0580030107.01.T01 |
| Chr5 | CDS | 106756381 | 106756575 | 106756381 | ID=MsG0580030107.01.T01:cds;Parent=MsG0580030107.01.T01 |
| Chr5 | CDS | 106755746 | 106756089 | 106755746 | ID=MsG0580030107.01.T01:cds;Parent=MsG0580030107.01.T01 |
| Chr5 | mRNA | 106755746 | 106756846 | 106755746 | ID=MsG0580030107.01.T02;Parent=MsG0580030107.01;Name=MsG0580030107.01.T02;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|366 |
| Chr5 | exon | 106755746 | 106756846 | 106755746 | ID=MsG0580030107.01.T02:exon:17265;Parent=MsG0580030107.01.T02 |
| Chr5 | CDS | 106755746 | 106756846 | 106755746 | ID=MsG0580030107.01.T02:cds;Parent=MsG0580030107.01.T02 |
| Chr5 | mRNA | 106755746 | 106756240 | 106755746 | ID=MsG0580030107.01.T03;Parent=MsG0580030107.01;Name=MsG0580030107.01.T03;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|0|1|0|164 |
| Chr5 | exon | 106755746 | 106756240 | 106755746 | ID=MsG0580030107.01.T03:exon:17266;Parent=MsG0580030107.01.T03 |
| Chr5 | CDS | 106755746 | 106756240 | 106755746 | ID=MsG0580030107.01.T03:cds;Parent=MsG0580030107.01.T03 |
| Gene Sequence |
>MsG0580030107.01.T02 ATGGAAAGGGTAATAAAGAGTGAATATATACATGATGATATTGCCTTCTCTATTCTCTCAAAATTACCTATTAAATCTTTCAAACGATTTGAATGTGTGTGCAAATCATGGTCTCTCTTGTTTGATAACCCTAACTTCATGAAGGTCTACGGCAAGAGTTTCTTAACAAAGGATCATTCTATTTATGACGATACATCTCTCCTCTTACATAAAAAAATTGATGGTCCTTGGGATGGTTATTATTGGGATGAAACATTTGAGTTATATTCCGTTTCTGGTAAAAGGTTTGAGAATATGGTCAAATTAGATTGGCCAAGTGTCAAAATAGATTTTAAAATGGAGTATGATTCTGGTTTTAATATTTTAGGTTCCAGAAGTGTTCATGGAACTCTTTGTCTATTTTGTGCATTTAAAACAAATATTATATTGTGGAATCCATCTACTAAGGAATTCAAAATCATTCCTCCTGGCTCGCTTTCTTGGGCTGTTGGGCATGCGTTTGGTTACGACAGTGTTAAAGAAGACTATAAAGTGATGCGTCTTGGTGTAGGGCAATCTGAGATATATAGTCTAAGGTGTAACTCTTGGAGAAAAATTGATGTCAATATGCAACCGATACGTAACAACTATTGGCACTATGACCAACTGTACACGGATGGACTTTCTCACTGGCTCAGTCAAAGTGAAACACATAATGTAGCATTGACTTCATTTGACTGGAGCAATGAGGTTTTCATTAAAACATCCTTACCTTCAGACACAGATTCAGTGTGGAAACATTTGGTGCTTTTAAATGGATCTATTACTTTGATCTTAAATCATACAGACACGGCTACGTTTCACATTTCAATTTTAGGTGAACTCGGTGTAATGGAATCATGGACTAAAATCTTCATTGTAGGGCCTTTTCCTTGCCTCGACTATCCTATCGGAGCGGGAAAGAAGGGTGATCTGTTTTTCAGAAAAAAAGACGGGGGGCTAGTTTGGTTTGATTTAAATTCCCAAACGATTGAGGACATTGGTGTTACAGCCGAACAATTTTGTTGTGAGATAGTAATTCATAAGGAAAGTCTTCTTCCATTTGAAGGAGAAAATATCTAA >MsG0580030107.01.T01 ATGGAAAGGGTAATAAAGAGTGAATATATACATGATGATATTGCCTTCTCTATTCTCTCAAAATTACCTATTAAATCTTTCAAACGATTTGAATGTGTGTGCAAATCATGGTCTCTCTTGTTTGATAACCCTAACTTCATGAAGGTCTACGGCAAGAGTTTCTTAACAAAGGATCATTCTATTTATGACGATACATCTCTCCTCTTACATAAAAAAATTGATGGTCCTTGGGATGTTTCTGGTAAAAGGTTTGAGAATATGGTCAAATTAGATTGGCCAAGTGTCAAAATAGATTTTAAAATGGAGTATGATTCTGGTTTTAATATTTTAGGTTCCAGAAGTGTTCATGGAACTCTTTGTCTATTTTGTGCATTTAAAACAAATATTATATTGTGGAATCCATCTACTAAGGAATTCAAAATCATTCCTCACACAGATTCAGTGTGGAAACATTTGGTGCTTTTAAATGGATCTATTACTTTGATCTTAAATCATACAGACACGGCTACGTTTCACATTTCAATTTTAGGTGAACTCGGTGTAATGGAATCATGGACTAAAATCTTCATTGTAGGGCCTTTTCCTTGCCTCGACTATCCTATCGGAGCGGGAAAGAAGGGTGATCTGTTTTTCAGAAAAAAAGACGGGGGGCTAGTTTGGTTTGATTTAAATTCCCAAACGATTGAGGACATTGGTGTTACAGCCGAACAATTTTGTTGTGAGATAGTAATTCATAAGGAAAGTCTTCTTCCATTTGAAGGAGAAAATATCTAA >MsG0580030107.01.T03 ATGCAACCGATACGTAACAACTATTGGCACTATGACCAACTGTACACGGATGGACTTTCTCACTGGCTCAGTCAAAGTGAAACACATAATGTAGCATTGACTTCATTTGACTGGAGCAATGAGGTTTTCATTAAAACATCCTTACCTTCAGACACAGATTCAGTGTGGAAACATTTGGTGCTTTTAAATGGATCTATTACTTTGATCTTAAATCATACAGACACGGCTACGTTTCACATTTCAATTTTAGGTGAACTCGGTGTAATGGAATCATGGACTAAAATCTTCATTGTAGGGCCTTTTCCTTGCCTCGACTATCCTATCGGAGCGGGAAAGAAGGGTGATCTGTTTTTCAGAAAAAAAGACGGGGGGCTAGTTTGGTTTGATTTAAATTCCCAAACGATTGAGGACATTGGTGTTACAGCCGAACAATTTTGTTGTGAGATAGTAATTCATAAGGAAAGTCTTCTTCCATTTGAAGGAGAAAATATCTAA |
| Protein sequence |
>MsG0580030107.01.T02 MERVIKSEYIHDDIAFSILSKLPIKSFKRFECVCKSWSLLFDNPNFMKVYGKSFLTKDHSIYDDTSLLLHKKIDGPWDGYYWDETFELYSVSGKRFENMVKLDWPSVKIDFKMEYDSGFNILGSRSVHGTLCLFCAFKTNIILWNPSTKEFKIIPPGSLSWAVGHAFGYDSVKEDYKVMRLGVGQSEIYSLRCNSWRKIDVNMQPIRNNYWHYDQLYTDGLSHWLSQSETHNVALTSFDWSNEVFIKTSLPSDTDSVWKHLVLLNGSITLILNHTDTATFHISILGELGVMESWTKIFIVGPFPCLDYPIGAGKKGDLFFRKKDGGLVWFDLNSQTIEDIGVTAEQFCCEIVIHKESLLPFEGENI* >MsG0580030107.01.T01 MERVIKSEYIHDDIAFSILSKLPIKSFKRFECVCKSWSLLFDNPNFMKVYGKSFLTKDHSIYDDTSLLLHKKIDGPWDVSGKRFENMVKLDWPSVKIDFKMEYDSGFNILGSRSVHGTLCLFCAFKTNIILWNPSTKEFKIIPHTDSVWKHLVLLNGSITLILNHTDTATFHISILGELGVMESWTKIFIVGPFPCLDYPIGAGKKGDLFFRKKDGGLVWFDLNSQTIEDIGVTAEQFCCEIVIHKESLLPFEGENI* >MsG0580030107.01.T03 MQPIRNNYWHYDQLYTDGLSHWLSQSETHNVALTSFDWSNEVFIKTSLPSDTDSVWKHLVLLNGSITLILNHTDTATFHISILGELGVMESWTKIFIVGPFPCLDYPIGAGKKGDLFFRKKDGGLVWFDLNSQTIEDIGVTAEQFCCEIVIHKESLLPFEGENI* |
