AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030314.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030314.01.T01 MTR_6g004420 75.000 220 3 2 1 220 1 168 1.68e-108 309
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030314.01.T01 AT3G27520 36.937 222 97 6 5 219 13 198 3.81e-36 126

Find 59 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTTGGGTTTGAAAATT+TGG 0.051627 6:-502072 None:intergenic
GAAGATGAAATCAAGGATTT+TGG 0.190683 6:+503230 MsG0680030314.01.T01:CDS
TTGTCGATTCCTTCTACTTC+TGG 0.230489 6:+502375 MsG0680030314.01.T01:CDS
TTCGGCTACGTCGCATAAAT+TGG 0.237128 6:-506267 None:intergenic
CAACATAAGCTAATTCTAAT+TGG 0.239132 6:-506596 None:intergenic
TTGGCCGGTGCGGTGCGGTC+TGG 0.253283 6:+503178 MsG0680030314.01.T01:CDS
ATTTAAAAGCATCCAACTTC+TGG 0.281553 6:+506563 MsG0680030314.01.T01:three_prime_UTR
CGTCGTCTTCGGATTCCATT+TGG 0.294775 6:-502086 None:intergenic
TTCTCTAGCGCGTTCTGATC+TGG 0.317517 6:-502195 None:intergenic
TGGCCGGTGCGGTGCGGTCT+GGG 0.319067 6:+503179 MsG0680030314.01.T01:CDS
CAATAGATAGAAGAAGAAAA+AGG 0.343186 6:+501992 None:intergenic
AAGCTCCCATTCGTCGTCTT+CGG 0.371753 6:-502097 None:intergenic
CTTTGTTTGATCTACCAATC+TGG 0.375451 6:+506327 MsG0680030314.01.T01:CDS
CGGTCAAAAGCGGCCGCTAT+TGG 0.389306 6:+503159 MsG0680030314.01.T01:CDS
GGCGCTGTTGAGGAGGGTTC+CGG 0.396139 6:-502174 None:intergenic
GTCGTCTTCGGATTCCATTT+GGG 0.399546 6:-502085 None:intergenic
AGATTAGACCATTGGCAGAT+TGG 0.406199 6:+506510 MsG0680030314.01.T01:three_prime_UTR
AGAAAGCTGCAATGCACTTA+AGG 0.416394 6:-502307 None:intergenic
TGAAAAGGAAATTGTTCAAT+GGG 0.430462 6:+502269 MsG0680030314.01.T01:CDS
GGTGCGGTGCGGTCTGGGAG+CGG 0.434513 6:+503184 MsG0680030314.01.T01:CDS
TTGTTTAGAACACATTGCTT+CGG 0.440413 6:-506285 None:intergenic
TCTTGATGATATTCTCTCTC+AGG 0.446892 6:+502416 MsG0680030314.01.T01:CDS
GGAATCCGAAGACGACGAAT+GGG 0.447320 6:+502092 MsG0680030314.01.T01:CDS
AAACTTGCAGGTGGAAGCTC+AGG 0.449746 6:+506223 MsG0680030314.01.T01:intron
TCAGCAGATCCAGAAGTAGA+AGG 0.457904 6:-502384 None:intergenic
CAAAAGCGGCCGCTATTGGC+CGG 0.465209 6:+503163 MsG0680030314.01.T01:CDS
ATGAAAAGGAAATTGTTCAA+TGG 0.466824 6:+502268 MsG0680030314.01.T01:CDS
CTGATCTGGCGCTGTTGAGG+AGG 0.476115 6:-502181 None:intergenic
AGTTGAGACGACGCCGTTTG+CGG 0.489787 6:-502149 None:intergenic
AGAAAATGAAGATGAAATCA+AGG 0.495928 6:+503223 MsG0680030314.01.T01:CDS
TGGAATCCGAAGACGACGAA+TGG 0.496694 6:+502091 MsG0680030314.01.T01:CDS
TCGTCTACAAACGCCGCAAA+CGG 0.498988 6:+502136 MsG0680030314.01.T01:CDS
CGCTATTGGCCGGTGCGGTG+CGG 0.501810 6:+503173 MsG0680030314.01.T01:CDS
GGATGACTCCAGCCAGAAGT+TGG 0.507093 6:-506575 None:intergenic
GCTTAAATCCAAGTATGAAA+AGG 0.514430 6:+502254 MsG0680030314.01.T01:CDS
GCTCCCAGACCGCACCGCAC+CGG 0.515324 6:-503182 None:intergenic
GGTAATTATGTCAAGTTCAA+AGG 0.516300 6:+506423 MsG0680030314.01.T01:three_prime_UTR
GCCGGTGCGTGTCAGATGGC+CGG 0.518316 6:+503134 MsG0680030314.01.T01:intron
TGTTAAATAGATTAGACCAT+TGG 0.520855 6:+506502 MsG0680030314.01.T01:three_prime_UTR
TGATCTGGCGCTGTTGAGGA+GGG 0.521322 6:-502180 None:intergenic
TGACATAATTACCTAGTGAA+TGG 0.533749 6:-506413 None:intergenic
GTTCTGATCTGGCGCTGTTG+AGG 0.533842 6:-502184 None:intergenic
AAAAGCATCCAACTTCTGGC+TGG 0.534898 6:+506567 MsG0680030314.01.T01:three_prime_UTR
GCGGCCGCTATTGGCCGGTG+CGG 0.537975 6:+503168 MsG0680030314.01.T01:CDS
AAAACTTGCATTAGCTCAAG+TGG 0.545556 6:-506356 None:intergenic
CGCACCGCACCGGCCAATAG+CGG 0.571042 6:-503172 None:intergenic
TCAAGTGGTGTAGACCAGAT+TGG 0.583060 6:-506341 None:intergenic
AATTATGTCAAGTTCAAAGG+AGG 0.592310 6:+506426 MsG0680030314.01.T01:three_prime_UTR
ATGGCCGGCGCGGTCAAAAG+CGG 0.593867 6:+503149 MsG0680030314.01.T01:CDS
GTTGAATGTAAAGAGTGAGA+AGG 0.595423 6:+506615 MsG0680030314.01.T01:three_prime_UTR
AATATTTACCAATCTGCCAA+TGG 0.601157 6:-506518 None:intergenic
TTGGAATGAAGCGCGAACGC+GGG 0.611428 6:-502053 None:intergenic
TGGGAGCTTTGCTACGACGA+CGG 0.619489 6:+502111 MsG0680030314.01.T01:CDS
ACGGCGTCGTCTCAACTCAC+CGG 0.636523 6:+502155 MsG0680030314.01.T01:CDS
TTTGGAATGAAGCGCGAACG+CGG 0.640443 6:-502054 None:intergenic
ATTATGTCAAGTTCAAAGGA+GGG 0.658492 6:+506427 MsG0680030314.01.T01:three_prime_UTR
TGCGTGTCAGATGGCCGGCG+CGG 0.688056 6:+503139 MsG0680030314.01.T01:intron
CAATGTGAAACTGCATGTTG+CGG 0.703399 6:+505741 MsG0680030314.01.T01:CDS
ATGAGATGATGACTATTGTG+CGG 0.723375 6:+506685 MsG0680030314.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAATAATAATAATA+AGG + Chr6:503543-503562 MsG0680030314.01.T01:intron 0.0%
!! TAAAATAATAATAATAATAA+GGG + Chr6:503544-503563 MsG0680030314.01.T01:intron 0.0%
!!! ATTTTTAATAAAAAATATAT+AGG + Chr6:505046-505065 MsG0680030314.01.T01:intron 0.0%
!!! ATGATTTTTGATTAATTTTA+AGG + Chr6:504992-505011 MsG0680030314.01.T01:intron 10.0%
!!! GAAGTATTAAAAAATATTTA+TGG + Chr6:505181-505200 MsG0680030314.01.T01:intron 10.0%
!!! TTATTTTTTATTTTTAAGTG+TGG - Chr6:503829-503848 None:intergenic 10.0%
!!! TTTAATTTCTGATTTAAATT+AGG - Chr6:503784-503803 None:intergenic 10.0%
!! AAAAACATTTATTGAAATGT+AGG + Chr6:504574-504593 MsG0680030314.01.T01:intron 15.0%
!! AAAAACATTTATTGAAATGT+AGG - Chr6:504593-504574 None:intergenic 15.0%
!! AAAACATTTATTGAAATGTA+GGG + Chr6:504575-504594 MsG0680030314.01.T01:intron 15.0%
!! AAAACATTTATTGAAATGTA+GGG - Chr6:504594-504575 None:intergenic 15.0%
!! AAAACATTTGTCATATATTA+TGG + Chr6:505588-505607 MsG0680030314.01.T01:intron 15.0%
!! AACCATAAATCTTAAATAAA+AGG - Chr6:504948-504967 None:intergenic 15.0%
!! AAGAAATAATTTCTACATTT+GGG + Chr6:505948-505967 MsG0680030314.01.T01:intron 15.0%
!! AATTTGAAAAATTGGTAAAT+TGG + Chr6:502835-502854 MsG0680030314.01.T01:intron 15.0%
!! ATTTGAAAAATTGGTAAATT+GGG + Chr6:502836-502855 MsG0680030314.01.T01:intron 15.0%
!! GTATTAAAAAATATTTATGG+AGG + Chr6:505184-505203 MsG0680030314.01.T01:intron 15.0%
!! TAAGAAATAATTTCTACATT+TGG + Chr6:505947-505966 MsG0680030314.01.T01:intron 15.0%
!!! TAAAATGTTTTTTTTTTGGA+AGG + Chr6:504348-504367 MsG0680030314.01.T01:intron 15.0%
!!! TACCTTTTATTTAAGATTTA+TGG + Chr6:504943-504962 MsG0680030314.01.T01:intron 15.0%
!! AAAGAAAGTTTGAAAATTTG+GGG + Chr6:502562-502581 MsG0680030314.01.T01:intron 20.0%
!! AAGAGAGATGTATTATTATT+AGG - Chr6:504650-504669 None:intergenic 20.0%
!! ACTGAAAGAAAAAAAAATGA+TGG - Chr6:503933-503952 None:intergenic 20.0%
!! AGAGAGATGTATTATTATTA+GGG - Chr6:504649-504668 None:intergenic 20.0%
!! AGTCTTCTAATTTGAAAAAT+TGG + Chr6:502827-502846 MsG0680030314.01.T01:intron 20.0%
!! ATCATGAACAAAATAGATAA+CGG - Chr6:502529-502548 None:intergenic 20.0%
!! GAAAGAAAGTTTGAAAATTT+GGG + Chr6:502561-502580 MsG0680030314.01.T01:intron 20.0%
!! TGAAAGAAAGTTTGAAAATT+TGG + Chr6:502560-502579 MsG0680030314.01.T01:intron 20.0%
!! TTTGAAAAATTGGTAAATTG+GGG + Chr6:502837-502856 MsG0680030314.01.T01:intron 20.0%
!! TTTGTCATATATTATGGATT+TGG + Chr6:505594-505613 MsG0680030314.01.T01:intron 20.0%
!!! ATTAGGGTTAAATATGTTTT+TGG - Chr6:504633-504652 None:intergenic 20.0%
!!! ATTTTGTTGAATAAAAGACA+AGG - Chr6:504919-504938 None:intergenic 20.0%
!!! GAAAGAAATTTTGTTTTTGT+GGG + Chr6:502464-502483 MsG0680030314.01.T01:intron 20.0%
!!! TAAATTAGGATTTTAACATG+TGG - Chr6:503770-503789 None:intergenic 20.0%
!!! TAGAATGAATTTCTGAAATT+AGG + Chr6:504281-504300 MsG0680030314.01.T01:intron 20.0%
!!! TATGTTTTGTTGATATTTGA+GGG + Chr6:504204-504223 MsG0680030314.01.T01:intron 20.0%
!!! TATTATTAACCAAGCATTTT+TGG - Chr6:503490-503509 None:intergenic 20.0%
!!! TCATTTCTTTTACAGTTAAT+TGG + Chr6:504131-504150 MsG0680030314.01.T01:intron 20.0%
!!! TGAAAGAAATTTTGTTTTTG+TGG + Chr6:502463-502482 MsG0680030314.01.T01:intron 20.0%
!!! TTTTTTTTGGAAGGAAAAAT+TGG + Chr6:504357-504376 MsG0680030314.01.T01:intron 20.0%
! AAAATATTTATGGAGGTTTC+TGG + Chr6:505191-505210 MsG0680030314.01.T01:intron 25.0%
! ACAAGTCTATAATTCTACAA+AGG - Chr6:506128-506147 None:intergenic 25.0%
! AGAAAATGAAGATGAAATCA+AGG + Chr6:503223-503242 MsG0680030314.01.T01:CDS 25.0%
! AGTATTTACATGGTAAGATA+TGG - Chr6:505787-505806 None:intergenic 25.0%
! ATATCTTACCATGTAAATAC+TGG + Chr6:505786-505805 MsG0680030314.01.T01:intron 25.0%
! ATGAAAAGGAAATTGTTCAA+TGG + Chr6:502268-502287 MsG0680030314.01.T01:CDS 25.0%
! ATGAGCTTAAATATTATGCA+TGG + Chr6:505278-505297 MsG0680030314.01.T01:intron 25.0%
! CAACATAAGCTAATTCTAAT+TGG - Chr6:506599-506618 None:intergenic 25.0%
! GAAGATGAAATCAAAGATAT+TGG + Chr6:503372-503391 MsG0680030314.01.T01:intron 25.0%
! TCATGAACAAAATAGATAAC+GGG - Chr6:502528-502547 None:intergenic 25.0%
! TCTTTCAAAATTACGAAGAA+TGG - Chr6:502450-502469 None:intergenic 25.0%
! TGAAAAGGAAATTGTTCAAT+GGG + Chr6:502269-502288 MsG0680030314.01.T01:CDS 25.0%
! TGTTAAATAGATTAGACCAT+TGG + Chr6:506502-506521 MsG0680030314.01.T01:three_prime_UTR 25.0%
! TTAAAGTCACGCAAAATATA+GGG + Chr6:504606-504625 MsG0680030314.01.T01:intron 25.0%
! TTAAAGTCACGCAAAATATA+GGG - Chr6:504625-504606 None:intergenic 25.0%
! TTTAGATATGTTTGTGTTGT+AGG + Chr6:504164-504183 MsG0680030314.01.T01:intron 25.0%
! TTTGTGTAAATTTACTGCTT+AGG + Chr6:502794-502813 MsG0680030314.01.T01:intron 25.0%
!! AACAATTTCCTTTTCATACT+TGG - Chr6:502265-502284 None:intergenic 25.0%
!! GTGTTTGAATTTTTCTTTTG+TGG + Chr6:502867-502886 MsG0680030314.01.T01:intron 25.0%
!! TATTATGGATTTGGATTAGA+CGG + Chr6:505603-505622 MsG0680030314.01.T01:intron 25.0%
!! TCTATTTTATACCATTCACT+AGG + Chr6:506402-506421 MsG0680030314.01.T01:three_prime_UTR 25.0%
!! TTGATTTCATCTTCATTTTC+TGG - Chr6:503224-503243 None:intergenic 25.0%
!! TTTAAAGTCACGCAAAATAT+AGG + Chr6:504605-504624 MsG0680030314.01.T01:intron 25.0%
!! TTTAAAGTCACGCAAAATAT+AGG - Chr6:504624-504605 None:intergenic 25.0%
!!! ATGTTTTGTTGATATTTGAG+GGG + Chr6:504205-504224 MsG0680030314.01.T01:intron 25.0%
!!! CTATGTTTTGTTGATATTTG+AGG + Chr6:504203-504222 MsG0680030314.01.T01:intron 25.0%
!!! TTTTTTCTTTGTGTTTCTGT+TGG + Chr6:502651-502670 MsG0680030314.01.T01:intron 25.0%
AAAACATTCACCGTATCTTA+TGG + Chr6:505320-505339 MsG0680030314.01.T01:intron 30.0%
AAGATGCCACTCAAAAATTA+CGG + Chr6:504454-504473 MsG0680030314.01.T01:intron 30.0%
AATATTTACCAATCTGCCAA+TGG - Chr6:506521-506540 None:intergenic 30.0%
AATTATGTCAAGTTCAAAGG+AGG + Chr6:506426-506445 MsG0680030314.01.T01:three_prime_UTR 30.0%
ACTAACGGAGTAAAAATGTA+GGG - Chr6:503647-503666 None:intergenic 30.0%
AGGATGCAACATGATAAAAA+AGG + Chr6:504310-504329 MsG0680030314.01.T01:intron 30.0%
ATAATGATGAAGATGGTGAT+GGG + Chr6:504241-504260 MsG0680030314.01.T01:intron 30.0%
ATATCATTAATGAGACCATG+TGG - Chr6:503741-503760 None:intergenic 30.0%
ATTATGTCAAGTTCAAAGGA+GGG + Chr6:506427-506446 MsG0680030314.01.T01:three_prime_UTR 30.0%
ATTCTTTCATTCACTTGTTC+TGG + Chr6:504725-504744 MsG0680030314.01.T01:intron 30.0%
GAGAAGTAGAAACATATTGT+TGG + Chr6:503446-503465 MsG0680030314.01.T01:intron 30.0%
GCTTAAATCCAAGTATGAAA+AGG + Chr6:502254-502273 MsG0680030314.01.T01:CDS 30.0%
GGTAATTATGTCAAGTTCAA+AGG + Chr6:506423-506442 MsG0680030314.01.T01:three_prime_UTR 30.0%
TACTAACGGAGTAAAAATGT+AGG - Chr6:503648-503667 None:intergenic 30.0%
TATAATGATGAAGATGGTGA+TGG + Chr6:504240-504259 MsG0680030314.01.T01:intron 30.0%
TCAAGCTATAATGATGAAGA+TGG + Chr6:504234-504253 MsG0680030314.01.T01:intron 30.0%
TGACATAATTACCTAGTGAA+TGG - Chr6:506416-506435 None:intergenic 30.0%
TGATCCAAATCCATAAGATA+CGG - Chr6:505333-505352 None:intergenic 30.0%
TTTAGTATTCTCAAACTTGC+AGG + Chr6:506211-506230 MsG0680030314.01.T01:intron 30.0%
TTTATCCAGCAAAAATTGTG+AGG + Chr6:504059-504078 MsG0680030314.01.T01:intron 30.0%
! AATTAAGTAGCCGTTATTTC+TGG + Chr6:506091-506110 MsG0680030314.01.T01:intron 30.0%
! ATAGATTGCGTCAATTTTGT+TGG + Chr6:502747-502766 MsG0680030314.01.T01:intron 30.0%
! GAAGATGAAATCAAGGATTT+TGG + Chr6:503230-503249 MsG0680030314.01.T01:CDS 30.0%
! TCCAAATTTTCAAACCCAAA+TGG + Chr6:502071-502090 MsG0680030314.01.T01:exon 30.0%
! TCCATTTGGGTTTGAAAATT+TGG - Chr6:502075-502094 None:intergenic 30.0%
! TTCATCTTCATTTTCTGGAT+CGG - Chr6:503219-503238 None:intergenic 30.0%
! TTGTTTAGAACACATTGCTT+CGG - Chr6:506288-506307 None:intergenic 30.0%
! TTTTACTCCGTTAGTACAAT+TGG + Chr6:503652-503671 MsG0680030314.01.T01:intron 30.0%
!! ATTTAAAAGCATCCAACTTC+TGG + Chr6:506563-506582 MsG0680030314.01.T01:three_prime_UTR 30.0%
!! ATTTTTGATGACTAGGCTAA+CGG + Chr6:503687-503706 MsG0680030314.01.T01:intron 30.0%
!! GAATTTCTGAAATTAGGCTT+GGG + Chr6:504287-504306 MsG0680030314.01.T01:intron 30.0%
!! GATGGGAATTGAGTTATTTT+TGG + Chr6:504258-504277 MsG0680030314.01.T01:intron 30.0%
!! TGAATTTCTGAAATTAGGCT+TGG + Chr6:504286-504305 MsG0680030314.01.T01:intron 30.0%
!!! GTTCAGTAGTTTTGTATCTT+TGG + Chr6:506646-506665 MsG0680030314.01.T01:three_prime_UTR 30.0%
!!! GTTTTGTATCTTTGGAATGT+TGG + Chr6:506654-506673 MsG0680030314.01.T01:three_prime_UTR 30.0%
AAAACTTGCATTAGCTCAAG+TGG - Chr6:506359-506378 None:intergenic 35.0%
AAATTGGTAAATTGGGGCTT+AGG + Chr6:502843-502862 MsG0680030314.01.T01:intron 35.0%
AAGACAGATCCAAAAATGCT+TGG + Chr6:503478-503497 MsG0680030314.01.T01:intron 35.0%
AATGTTGATGTGGCATATTG+TGG - Chr6:504483-504502 None:intergenic 35.0%
ACTAACGGAGCAAAAATGTA+GGG + Chr6:504546-504565 MsG0680030314.01.T01:intron 35.0%
ACTATCCGATCTCAAATCAA+TGG + Chr6:505436-505455 MsG0680030314.01.T01:intron 35.0%
ACTATGTCTGAAGGAAAAAG+AGG - Chr6:504026-504045 None:intergenic 35.0%
AGATGCCACTCAAAAATTAC+GGG + Chr6:504455-504474 MsG0680030314.01.T01:intron 35.0%
AGGTTGTTCTATGATGAAGA+AGG - Chr6:503982-504001 None:intergenic 35.0%
ATCCTGGAACAAGAAGAAAA+GGG - Chr6:503859-503878 None:intergenic 35.0%
ATGAGATGATGACTATTGTG+CGG + Chr6:506685-506704 MsG0680030314.01.T01:three_prime_UTR 35.0%
ATGATGACATGTATGTATGC+TGG + Chr6:502705-502724 MsG0680030314.01.T01:intron 35.0%
ATGTTGATGTGGCATATTGT+GGG - Chr6:504482-504501 None:intergenic 35.0%
ATTACACAGTTAACCGATCT+CGG - Chr6:505681-505700 None:intergenic 35.0%
ATTCTACAAAGGATGTTCCA+AGG - Chr6:506117-506136 None:intergenic 35.0%
CCTACTCATCATTTCTCAAA+GGG - Chr6:506012-506031 None:intergenic 35.0%
GCAGTAAATTTACACAAACC+AGG - Chr6:502792-502811 None:intergenic 35.0%
GCCTAGTCATCAAAAATAGA+CGG - Chr6:503684-503703 None:intergenic 35.0%
GTTGAATGTAAAGAGTGAGA+AGG + Chr6:506615-506634 MsG0680030314.01.T01:three_prime_UTR 35.0%
GTTGATTGAACTATGTCTGA+AGG - Chr6:504035-504054 None:intergenic 35.0%
TACTAACGGAGCAAAAATGT+AGG + Chr6:504545-504564 MsG0680030314.01.T01:intron 35.0%
TATCTGCACCAGTATTTACA+TGG - Chr6:505797-505816 None:intergenic 35.0%
TATCTTGACCGTAGTTACTT+TGG - Chr6:505492-505511 None:intergenic 35.0%
TCCTACTCATCATTTCTCAA+AGG - Chr6:506013-506032 None:intergenic 35.0%
TCTAACCATAGTTGTCAATG+CGG - Chr6:505156-505175 None:intergenic 35.0%
TGAACTGCATATGTAGTGAA+TGG + Chr6:506157-506176 MsG0680030314.01.T01:intron 35.0%
TGAAGTCAATCTATGAGAAG+AGG - Chr6:504002-504021 None:intergenic 35.0%
TGTCATGTGCATTAAGAGTA+AGG - Chr6:504869-504888 None:intergenic 35.0%
TTACATGGTAAGATATGGAG+CGG - Chr6:505782-505801 None:intergenic 35.0%
TTAGGAATCATTGCGTACAT+GGG + Chr6:504094-504113 MsG0680030314.01.T01:intron 35.0%
TTCACCGTATCTTATGGATT+TGG + Chr6:505326-505345 MsG0680030314.01.T01:intron 35.0%
TTCTTCCAATTCCAAACACT+CGG - Chr6:503029-503048 None:intergenic 35.0%
TTTGTTGATATTTGAGGGGA+AGG + Chr6:504209-504228 MsG0680030314.01.T01:intron 35.0%
! ATTTTCGATGACTAGGCTAA+CGG - Chr6:504506-504525 None:intergenic 35.0%
! CTTTGTTTGATCTACCAATC+TGG + Chr6:506327-506346 MsG0680030314.01.T01:CDS 35.0%
! TCTTGATGATATTCTCTCTC+AGG + Chr6:502416-502435 MsG0680030314.01.T01:CDS 35.0%
! TTTGTGTTTCTGTTGGATGT+TGG + Chr6:502658-502677 MsG0680030314.01.T01:intron 35.0%
! TTTTGCTCCGTTAGTACAAT+TGG - Chr6:504541-504560 None:intergenic 35.0%
!! AATTTGGGGTTTTTGTGAAG+TGG + Chr6:502576-502595 MsG0680030314.01.T01:intron 35.0%
!! ATTTGGGGTTTTTGTGAAGT+GGG + Chr6:502577-502596 MsG0680030314.01.T01:intron 35.0%
!! TGGTGCAGATATTTTGAGAA+AGG + Chr6:505806-505825 MsG0680030314.01.T01:intron 35.0%
!! TTTCAAGCTTTAAGCGATCT+TGG - Chr6:505462-505481 None:intergenic 35.0%
!!! AAATCAAGGATTTTGGTTGC+TGG + Chr6:503237-503256 MsG0680030314.01.T01:intron 35.0%
!!! GCGTCAATTTTGTTGGTTTT+AGG + Chr6:502754-502773 MsG0680030314.01.T01:intron 35.0%
AAGAAGACAGATCAAGGATG+AGG + Chr6:503346-503365 MsG0680030314.01.T01:intron 40.0%
ACCACAATGTCGAAATTGCT+AGG - Chr6:502983-503002 None:intergenic 40.0%
ACCTAGCAATTTCGACATTG+TGG + Chr6:502979-502998 MsG0680030314.01.T01:intron 40.0%
ACTGTGTGCCAAAGTAACTA+CGG + Chr6:505481-505500 MsG0680030314.01.T01:intron 40.0%
AGAAAGCTGCAATGCACTTA+AGG - Chr6:502310-502329 None:intergenic 40.0%
AGATTAGACCATTGGCAGAT+TGG + Chr6:506510-506529 MsG0680030314.01.T01:three_prime_UTR 40.0%
AGTATTCTCAAACTTGCAGG+TGG + Chr6:506214-506233 MsG0680030314.01.T01:intron 40.0%
ATGAAGATGACGATGATGCT+GGG - Chr6:503903-503922 None:intergenic 40.0%
CAATGTGAAACTGCATGTTG+CGG + Chr6:505741-505760 MsG0680030314.01.T01:CDS 40.0%
CTTAGGAATCATTGCGTACA+TGG + Chr6:504093-504112 MsG0680030314.01.T01:intron 40.0%
GAAACTCACGCATAATTGTG+AGG + Chr6:502925-502944 MsG0680030314.01.T01:intron 40.0%
GATCCTGGAACAAGAAGAAA+AGG - Chr6:503860-503879 None:intergenic 40.0%
GATGCCACTCAAAAATTACG+GGG + Chr6:504456-504475 MsG0680030314.01.T01:intron 40.0%
GCAAGGACCAATTGTACTAA+CGG + Chr6:504531-504550 MsG0680030314.01.T01:intron 40.0%
GCAAGGACCAATTGTACTAA+CGG - Chr6:504550-504531 None:intergenic 40.0%
GCATTGAGAGAATGAAAGAC+AGG - Chr6:503515-503534 None:intergenic 40.0%
GCCTAGTCATCGAAAATAGA+CGG + Chr6:504509-504528 MsG0680030314.01.T01:intron 40.0%
GTCATCAAAAATAGACGGCA+AGG - Chr6:503679-503698 None:intergenic 40.0%
GTGAGGCTTAGATTGTTCTT+AGG + Chr6:504076-504095 MsG0680030314.01.T01:intron 40.0%
GTTGTTTACCACCTTGTCTA+CGG - Chr6:502894-502913 None:intergenic 40.0%
TACATGGTAAGATATGGAGC+GGG - Chr6:505781-505800 None:intergenic 40.0%
TAGTTGTCAATGCGGAAATG+CGG - Chr6:505148-505167 None:intergenic 40.0%
TCTCGGCCATCAATTCAAAT+CGG - Chr6:505664-505683 None:intergenic 40.0%
TCTTCCAATTCCAAACACTC+GGG - Chr6:503028-503047 None:intergenic 40.0%
TGCCAAAAAAAACGCTATCG+CGG - Chr6:503097-503116 None:intergenic 40.0%
TGGAAGAAGAAGACAGATCA+AGG + Chr6:503340-503359 MsG0680030314.01.T01:intron 40.0%
TGTTCCAAGGCCAGAAATAA+CGG - Chr6:506104-506123 None:intergenic 40.0%
! ATCCCTTTTCTTCTTGTTCC+AGG + Chr6:503854-503873 MsG0680030314.01.T01:intron 40.0%
! CATTTCCGCATTGACAACTA+TGG + Chr6:505148-505167 MsG0680030314.01.T01:intron 40.0%
! CCCTTTGAGAAATGATGAGT+AGG + Chr6:506009-506028 MsG0680030314.01.T01:intron 40.0%
! CTAAGCCTCACAATTTTTGC+TGG - Chr6:504067-504086 None:intergenic 40.0%
! GATTGTTTGTGTCACGCTTT+TGG + Chr6:506040-506059 MsG0680030314.01.T01:intron 40.0%
! GTGAAGTGGGTCTGAAATTT+GGG + Chr6:502590-502609 MsG0680030314.01.T01:intron 40.0%
! TGTGAAGTGGGTCTGAAATT+TGG + Chr6:502589-502608 MsG0680030314.01.T01:intron 40.0%
! TTTAGGTTCAGCTGTAAACC+TGG + Chr6:502771-502790 MsG0680030314.01.T01:intron 40.0%
! TTTGAATTGATGGCCGAGAT+CGG + Chr6:505665-505684 MsG0680030314.01.T01:intron 40.0%
!! ACTTCTTAACTTGTGCCTTG+AGG - Chr6:505849-505868 None:intergenic 40.0%
!! CTTCTTAACTTGTGCCTTGA+GGG - Chr6:505848-505867 None:intergenic 40.0%
!! CTTGGCCATTGATTTGAGAT+CGG - Chr6:505444-505463 None:intergenic 40.0%
!! TTGTCGATTCCTTCTACTTC+TGG + Chr6:502375-502394 MsG0680030314.01.T01:CDS 40.0%
!! TTTCTGAAATTAGGCTTGGG+AGG + Chr6:504290-504309 MsG0680030314.01.T01:intron 40.0%
!!! GCCGTCTATTTTTGATGACT+AGG + Chr6:503680-503699 MsG0680030314.01.T01:intron 40.0%
!!! TTTTTGAGTGGCATCTTGTG+TGG - Chr6:504451-504470 None:intergenic 40.0%
AATGTTAACTCGTGCCCTCA+AGG + Chr6:505831-505850 MsG0680030314.01.T01:intron 45.0%
ACTTTACAGACCCGAGTGTT+TGG + Chr6:503015-503034 MsG0680030314.01.T01:intron 45.0%
AGAGAGAGAGAAAGAGAGAG+AGG + Chr6:503302-503321 MsG0680030314.01.T01:intron 45.0%
AGAGAGAGAGAGAAAGGATG+AGG + Chr6:503414-503433 MsG0680030314.01.T01:intron 45.0%
AGAGAGAGAGAGAGAGAGAA+AGG + Chr6:503294-503313 MsG0680030314.01.T01:intron 45.0%
AGAGAGAGGTAGAAAACGCA+TGG + Chr6:503316-503335 MsG0680030314.01.T01:intron 45.0%
AGGCTAACGGAATGTTGATG+TGG - Chr6:504493-504512 None:intergenic 45.0%
CTAGGTTAGACACCTTGACT+CGG - Chr6:502965-502984 None:intergenic 45.0%
GACATTGTGGTGAGTTTAGC+TGG + Chr6:502992-503011 MsG0680030314.01.T01:intron 45.0%
GATGAAGATGACGATGATGC+TGG - Chr6:503904-503923 None:intergenic 45.0%
GCCAAAAAAAACGCTATCGC+GGG - Chr6:503096-503115 None:intergenic 45.0%
GCCATCAATTCAAATCGGAC+GGG - Chr6:505659-505678 None:intergenic 45.0%
GGCCATCAATTCAAATCGGA+CGG - Chr6:505660-505679 None:intergenic 45.0%
GTCATCGAAAATAGACGGCA+AGG + Chr6:504514-504533 MsG0680030314.01.T01:intron 45.0%
GTCGTCTTCGGATTCCATTT+GGG - Chr6:502088-502107 None:intergenic 45.0%
TAGGTTAGACACCTTGACTC+GGG - Chr6:502964-502983 None:intergenic 45.0%
TCAAGTGGTGTAGACCAGAT+TGG - Chr6:506344-506363 None:intergenic 45.0%
TCAGCAGATCCAGAAGTAGA+AGG - Chr6:502387-502406 None:intergenic 45.0%
TTCGGCTACGTCGCATAAAT+TGG - Chr6:506270-506289 None:intergenic 45.0%
TTGCTTAACATGTGCCCCAA+GGG + Chr6:505971-505990 MsG0680030314.01.T01:intron 45.0%
! ATTTTCTGGATCGGCGCTTT+TGG - Chr6:503210-503229 None:intergenic 45.0%
! GCCGTCTATTTTCGATGACT+AGG - Chr6:504513-504532 None:intergenic 45.0%
! TCTTTTGTGGTCCGTAGACA+AGG + Chr6:502880-502899 MsG0680030314.01.T01:intron 45.0%
! TGATGTTGGTAGAGAGATCC+TGG - Chr6:503875-503894 None:intergenic 45.0%
!! AAAAGCATCCAACTTCTGGC+TGG + Chr6:506567-506586 MsG0680030314.01.T01:three_prime_UTR 45.0%
!! TCTCAGCCATCGATTCAGAT+CGG - Chr6:505395-505414 None:intergenic 45.0%
!!! TTTATAAAATGTTTTTTTTT+TGG + Chr6:504344-504363 MsG0680030314.01.T01:intron 5.0%
AAACTTGCAGGTGGAAGCTC+AGG + Chr6:506223-506242 MsG0680030314.01.T01:intron 50.0%
AAGATGACGATGATGCTGGG+TGG - Chr6:503900-503919 None:intergenic 50.0%
AAGCTCCCATTCGTCGTCTT+CGG - Chr6:502100-502119 None:intergenic 50.0%
AGAGGTAGAAAACGCATGGC+TGG + Chr6:503320-503339 MsG0680030314.01.T01:intron 50.0%
CACGCATAATTGTGAGGTCC+TGG + Chr6:502931-502950 MsG0680030314.01.T01:intron 50.0%
CGTACATGGGATTCTCTCAG+TGG + Chr6:504107-504126 MsG0680030314.01.T01:intron 50.0%
CGTCGTCTTCGGATTCCATT+TGG - Chr6:502089-502108 None:intergenic 50.0%
GGAATCCGAAGACGACGAAT+GGG + Chr6:502092-502111 MsG0680030314.01.T01:CDS 50.0%
GTTGCTTAACATGTGCCCCA+AGG + Chr6:505970-505989 MsG0680030314.01.T01:intron 50.0%
TCGTCTACAAACGCCGCAAA+CGG + Chr6:502136-502155 MsG0680030314.01.T01:CDS 50.0%
TGGAATCCGAAGACGACGAA+TGG + Chr6:502091-502110 MsG0680030314.01.T01:CDS 50.0%
TGTCGTCCGATCTGAATCGA+TGG + Chr6:505386-505405 MsG0680030314.01.T01:intron 50.0%
TTCTCTAGCGCGTTCTGATC+TGG - Chr6:502198-502217 None:intergenic 50.0%
TTTGTGGTCCGTAGACAAGG+TGG + Chr6:502883-502902 MsG0680030314.01.T01:intron 50.0%
! CAGACCCGAGTGTTTGGAAT+TGG + Chr6:503021-503040 MsG0680030314.01.T01:intron 50.0%
! GCCCGTCCGATTTGAATTGA+TGG + Chr6:505655-505674 MsG0680030314.01.T01:intron 50.0%
! TTTGGAATGAAGCGCGAACG+CGG - Chr6:502057-502076 None:intergenic 50.0%
!! GTAGCCGTTATTTCTGGCCT+TGG + Chr6:506097-506116 MsG0680030314.01.T01:intron 50.0%
!! GTGTCACGCTTTTGGTGCAT+TGG + Chr6:506048-506067 MsG0680030314.01.T01:intron 50.0%
!! TGGGCCCCGTAATTTTTGAG+TGG - Chr6:504463-504482 None:intergenic 50.0%
!!! GCCCGCGATAGCGTTTTTTT+TGG + Chr6:503092-503111 MsG0680030314.01.T01:intron 50.0%
AGTTGAGACGACGCCGTTTG+CGG - Chr6:502152-502171 None:intergenic 55.0%
CTGGATTCGAACCCGAGTCA+AGG + Chr6:502950-502969 MsG0680030314.01.T01:intron 55.0%
GAGACCATGTGGCATCCTAG+TGG - Chr6:503730-503749 None:intergenic 55.0%
GATGCTGGGTGGTGTGATGT+TGG - Chr6:503889-503908 None:intergenic 55.0%
GGATGACTCCAGCCAGAAGT+TGG - Chr6:506578-506597 None:intergenic 55.0%
GGCCACCCCAAAATTTGACG+CGG - Chr6:505117-505136 None:intergenic 55.0%
GTGGCATCTTGTGTGGACAC+AGG - Chr6:504444-504463 None:intergenic 55.0%
GTTCTGATCTGGCGCTGTTG+AGG - Chr6:502187-502206 None:intergenic 55.0%
TGATCTGGCGCTGTTGAGGA+GGG - Chr6:502183-502202 None:intergenic 55.0%
TGGGAGCTTTGCTACGACGA+CGG + Chr6:502111-502130 MsG0680030314.01.T01:CDS 55.0%
TTGGAATGAAGCGCGAACGC+GGG - Chr6:502056-502075 None:intergenic 55.0%
!! CTTGACTCGGGTTCGAATCC+AGG - Chr6:502952-502971 None:intergenic 55.0%
!!! GCGTAACGGGCGCTTTTCAA+TGG - Chr6:505080-505099 None:intergenic 55.0%
ACGGCGTCGTCTCAACTCAC+CGG + Chr6:502155-502174 MsG0680030314.01.T01:CDS 60.0%
CAAAAGCGGCCGCTATTGGC+CGG + Chr6:503163-503182 MsG0680030314.01.T01:CDS 60.0%
CCCAAAATTTGACGCGGCGC+GGG - Chr6:505111-505130 None:intergenic 60.0%
CCCCAAAATTTGACGCGGCG+CGG - Chr6:505112-505131 None:intergenic 60.0%
CGGTCAAAAGCGGCCGCTAT+TGG + Chr6:503159-503178 MsG0680030314.01.T01:CDS 60.0%
CTGATCTGGCGCTGTTGAGG+AGG - Chr6:502184-502203 None:intergenic 60.0%
GACGCCACTAGGATGCCACA+TGG + Chr6:503723-503742 MsG0680030314.01.T01:intron 60.0%
GATGCCACCTAGACGCCACT+AGG + Chr6:503712-503731 MsG0680030314.01.T01:intron 60.0%
GCATCCTAGTGGCGTCTAGG+TGG - Chr6:503719-503738 None:intergenic 60.0%
GTCAATGCGGAAATGCGGCG+CGG - Chr6:505143-505162 None:intergenic 60.0%
GTGGCATCCTAGTGGCGTCT+AGG - Chr6:503722-503741 None:intergenic 60.0%
! ACCTCCGCGTTGATCTGTGC+CGG + Chr6:503116-503135 MsG0680030314.01.T01:intron 60.0%
! ACGGGCGCTTTTCAATGGCG+CGG - Chr6:505075-505094 None:intergenic 60.0%
! CCCGCGCCGCGTCAAATTTT+GGG + Chr6:505108-505127 MsG0680030314.01.T01:intron 60.0%
! CCGCGCCGCGTCAAATTTTG+GGG + Chr6:505109-505128 MsG0680030314.01.T01:intron 60.0%
! TCCCGCGCCGCGTCAAATTT+TGG + Chr6:505107-505126 MsG0680030314.01.T01:intron 60.0%
!! CGCCGCGTCAAATTTTGGGG+TGG + Chr6:505112-505131 MsG0680030314.01.T01:intron 60.0%
ACCGGCACAGATCAACGCGG+AGG - Chr6:503120-503139 None:intergenic 65.0%
ATGGCCGGCGCGGTCAAAAG+CGG + Chr6:503149-503168 MsG0680030314.01.T01:CDS 65.0%
ATTTGACGCGGCGCGGGATG+CGG - Chr6:505105-505124 None:intergenic 65.0%
GGCGCTGTTGAGGAGGGTTC+CGG - Chr6:502177-502196 None:intergenic 65.0%
! CGCACCGGCACAGATCAACG+CGG - Chr6:503123-503142 None:intergenic 65.0%
! CTGTGCCGGTGCGTGTCAGA+TGG + Chr6:503130-503149 MsG0680030314.01.T01:intron 65.0%
ACGCTATCGCGGGCGCGAAG+CGG - Chr6:503086-503105 None:intergenic 70.0%
CGCTATTGGCCGGTGCGGTG+CGG + Chr6:503173-503192 MsG0680030314.01.T01:CDS 70.0%
GCCGGCCATCTGACACGCAC+CGG - Chr6:503138-503157 None:intergenic 70.0%
GCCGGTGCGTGTCAGATGGC+CGG + Chr6:503134-503153 MsG0680030314.01.T01:intron 70.0%
TGCGTGTCAGATGGCCGGCG+CGG + Chr6:503139-503158 MsG0680030314.01.T01:intron 70.0%
TTTGACGCGGCGCGGGATGC+GGG - Chr6:505104-505123 None:intergenic 70.0%
! CGCACCGCACCGGCCAATAG+CGG - Chr6:503175-503194 None:intergenic 70.0%
CGCGGGATGCGGGCGCGTAA+CGG - Chr6:505094-505113 None:intergenic 75.0%
CGCTATCGCGGGCGCGAAGC+GGG - Chr6:503085-503104 None:intergenic 75.0%
GCGGCCGCTATTGGCCGGTG+CGG + Chr6:503168-503187 MsG0680030314.01.T01:CDS 75.0%
GCGGGATGCGGGCGCGTAAC+GGG - Chr6:505093-505112 None:intergenic 75.0%
GCTCCCAGACCGCACCGCAC+CGG - Chr6:503185-503204 None:intergenic 75.0%
GGTGCGGTGCGGTCTGGGAG+CGG + Chr6:503184-503203 MsG0680030314.01.T01:CDS 75.0%
TATCGCGGGCGCGAAGCGGG+CGG - Chr6:503082-503101 None:intergenic 75.0%
TGCGGAAATGCGGCGCGGCG+CGG - Chr6:505138-505157 None:intergenic 75.0%
TGGCCGGTGCGGTGCGGTCT+GGG + Chr6:503179-503198 MsG0680030314.01.T01:CDS 75.0%
TTGGCCGGTGCGGTGCGGTC+TGG + Chr6:503178-503197 MsG0680030314.01.T01:CDS 75.0%
!!! GCGGCCGCTTTTGACCGCGC+CGG - Chr6:503156-503175 None:intergenic 75.0%
! CGGGCGCGAAGCGGGCGGCG+CGG - Chr6:503077-503096 None:intergenic 90.0%
Chromosome Type Strat End Strand Name
Chr6 gene 501997 506802 501997 ID=MsG0680030314.01;Name=MsG0680030314.01
Chr6 mRNA 501997 506802 501997 ID=MsG0680030314.01.T01;Parent=MsG0680030314.01;Name=MsG0680030314.01.T01;_AED=0.38;_eAED=0.46;_QI=93|0|0.5|1|1|1|4|408|220
Chr6 exon 501997 502437 501997 ID=MsG0680030314.01.T01:exon:2089;Parent=MsG0680030314.01.T01
Chr6 exon 503149 503251 503149 ID=MsG0680030314.01.T01:exon:2090;Parent=MsG0680030314.01.T01
Chr6 exon 505713 505762 505713 ID=MsG0680030314.01.T01:exon:2091;Parent=MsG0680030314.01.T01
Chr6 exon 506233 506802 506233 ID=MsG0680030314.01.T01:exon:2092;Parent=MsG0680030314.01.T01
Chr6 five_prime_UTR 501997 502089 501997 ID=MsG0680030314.01.T01:five_prime_utr;Parent=MsG0680030314.01.T01
Chr6 CDS 502090 502437 502090 ID=MsG0680030314.01.T01:cds;Parent=MsG0680030314.01.T01
Chr6 CDS 503149 503251 503149 ID=MsG0680030314.01.T01:cds;Parent=MsG0680030314.01.T01
Chr6 CDS 505713 505762 505713 ID=MsG0680030314.01.T01:cds;Parent=MsG0680030314.01.T01
Chr6 CDS 506233 506394 506233 ID=MsG0680030314.01.T01:cds;Parent=MsG0680030314.01.T01
Chr6 three_prime_UTR 506395 506802 506395 ID=MsG0680030314.01.T01:three_prime_utr;Parent=MsG0680030314.01.T01
Gene Sequence

>MsG0680030314.01.T01

ATGGAATCCGAAGACGACGAATGGGAGCTTTGCTACGACGACGGCTTCGTCTACAAACGCCGCAAACGGCGTCGTCTCAACTCACCGGAACCCTCCTCAACAGCGCCAGATCAGAACGCGCTAGAGAAGTTACGAAAAGAACGAAAGAAAACGACACTTTTGAAGCTTAAATCCAAGTATGAAAAGGAAATTGTTCAATGGGAACATTTATCAAACACCTTAAGTGCATTGCAGCTTTCTTCTTCAAATCAGCTTAATGAGCAGCAACAGAAGCAAAATCAAAACTTGTCGATTCCTTCTACTTCTGGATCTGCTGAATCGTCTCTTCTTGATGATATTCTCTCTCAGATGGCCGGCGCGGTCAAAAGCGGCCGCTATTGGCCGGTGCGGTGCGGTCTGGGAGCGGCCAAAAGCGCCGATCCAGAAAATGAAGATGAAATCAAGGATTTTGATCTCAAATCAACGAGTAAGATTACTTACAATGTGAAACTGCATGTTGCGGTGGAAGCTCAGGAAGCAATCATTCATGACATTTCCAATTTATGCGACGTAGCCGAAGCAATGTGTTCTAAACAAGAAGAACAATATAAACAATCTTTGTTTGATCTACCAATCTGGTCTACACCACTTGAGCTAATGCAAGTTTTATGCTATGATGATTGA
Protein sequence

>MsG0680030314.01.T01

MESEDDEWELCYDDGFVYKRRKRRRLNSPEPSSTAPDQNALEKLRKERKKTTLLKLKSKYEKEIVQWEHLSNTLSALQLSSSNQLNEQQQKQNQNLSIPSTSGSAESSLLDDILSQMAGAVKSGRYWPVRCGLGAAKSADPENEDEIKDFDLKSTSKITYNVKLHVAVEAQEAIIHDISNLCDVAEAMCSKQEEQYKQSLFDLPIWSTPLELMQVLCYDD*