AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030950.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030950.01.T01 MTR_6g015020 90.714 280 24 1 1 280 1 278 7.75e-167 463
MsG0680030950.01.T01 MTR_7g013100 59.532 299 90 7 1 280 1 287 1.52e-104 306
MsG0680030950.01.T01 MTR_7g080290 56.364 220 88 4 62 280 64 276 4.04e-66 207
MsG0680030950.01.T01 MTR_6g014880 58.974 78 29 1 155 232 1 75 4.95e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030950.01.T01 AT5G15530 47.973 296 97 12 1 280 1 255 3.43e-63 199
MsG0680030950.01.T01 AT5G16390 49.160 238 99 5 55 280 53 280 4.13e-58 187
MsG0680030950.01.T01 AT5G16390 47.847 209 87 5 55 251 53 251 3.65e-43 148

Find 86 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGTTCCTTCTTCCCTTT+TGG 0.121552 6:+12066956 MsG0680030950.01.T01:CDS
CACCAGAGACACTCCCATTT+TGG 0.190050 6:-12068535 None:intergenic
TTCAGCTTCATTTGGAATTC+AGG 0.228329 6:+12067058 MsG0680030950.01.T01:CDS
TAGAAGAAAACATTAATTAA+TGG 0.248480 6:-12070668 None:intergenic
TCAATAAATTTCTGATTAAC+AGG 0.266233 6:-12070493 None:intergenic
AAGGAGAAAGAAAACCAAAA+TGG 0.288031 6:+12068521 MsG0680030950.01.T01:CDS
TCTGCTGATTCAGCTTCATT+TGG 0.288792 6:+12067050 MsG0680030950.01.T01:CDS
TTGAAGAATTCTCAATCATT+TGG 0.310506 6:+12067020 MsG0680030950.01.T01:CDS
GGAAGAAGGAACAGCACATT+TGG 0.323479 6:-12066949 None:intergenic
AGGTCCAGGGCACCTGTAAA+AGG 0.353246 6:-12069372 None:intergenic
TGTGGGTCTACGAGATTATA+AGG 0.359362 6:-12066807 None:intergenic
TCCTTTGGTGAAGCAACATT+AGG 0.383801 6:-12068503 None:intergenic
GAAGAAGGAACAGCACATTT+GGG 0.396829 6:-12066948 None:intergenic
TTGTCGCAGGCTGATCAATC+AGG 0.415128 6:+12070028 MsG0680030950.01.T01:intron
AATGGAGGTTCGCCAGGTCC+AGG 0.421870 6:-12069386 None:intergenic
TCGAAACGAGTCTCACTTGT+TGG 0.423245 6:+12070442 MsG0680030950.01.T01:three_prime_UTR
GACTGCGGCATAGCCGTGGC+TGG 0.425804 6:-12069185 None:intergenic
AGTTGTGCTTGAACCTTGAC+AGG 0.434402 6:-12068309 None:intergenic
CATAAGCATCTGCCGATGGC+GGG 0.440146 6:-12069250 None:intergenic
ACTTTGAAATGAGGTTCCTT+AGG 0.440284 6:-12070420 None:intergenic
AACCAAAATGGGAGTGTCTC+TGG 0.440368 6:+12068533 MsG0680030950.01.T01:CDS
TGTTCACATACCTGGACAAA+TGG 0.445104 6:-12069404 None:intergenic
AACAAGATCTTGTTTCAAAG+TGG 0.445857 6:+12066990 MsG0680030950.01.T01:CDS
GGGAGTGTCTCTGGTGGCAC+TGG 0.457886 6:+12068542 MsG0680030950.01.T01:CDS
TCAAGTTGTTTGCATTATTG+AGG 0.458853 6:+12069854 MsG0680030950.01.T01:CDS
TGTTAGATTTCAATCCCAAA+AGG 0.460081 6:-12066971 None:intergenic
TTCAAAGGTGGCGAGCTTGT+AGG 0.463522 6:-12069275 None:intergenic
AGGAGAAAGAAAACCAAAAT+GGG 0.464491 6:+12068522 MsG0680030950.01.T01:CDS
GGCATAGCCGTGGCTGGTGG+AGG 0.467033 6:-12069179 None:intergenic
CATGGAACTATTACGAAAAG+AGG 0.469679 6:-12070393 None:intergenic
AAATAATGAAAATTGTTATG+TGG 0.473773 6:-12066825 None:intergenic
AATAATGAAAATTGTTATGT+GGG 0.477644 6:-12066824 None:intergenic
TATTTCCATGGCTAGACTTG+TGG 0.479550 6:+12069079 MsG0680030950.01.T01:intron
TTCAATCCCAAAAGGGAAGA+AGG 0.481168 6:-12066963 None:intergenic
CAAGAAACAATTTCCTGTCA+AGG 0.490969 6:+12068296 MsG0680030950.01.T01:CDS
TGGACAAATGGAGGTTCGCC+AGG 0.492799 6:-12069392 None:intergenic
CTCGTTTCGACTTTGAAATG+AGG 0.495638 6:-12070429 None:intergenic
TAAGCATCTGCCGATGGCGG+GGG 0.496000 6:-12069248 None:intergenic
ATATCTCTAGATAAATTAAG+AGG 0.500277 6:-12070572 None:intergenic
GCAAGAACATAACATCCAAC+AGG 0.501865 6:-12070695 None:intergenic
GTGGCGGCGAAGGAAGTGTC+GGG 0.508506 6:-12069226 None:intergenic
TGAGGTTCCTTAGGTACTCA+TGG 0.510365 6:-12070411 None:intergenic
ACAAGATCTTGTTTCAAAGT+GGG 0.510434 6:+12066991 MsG0680030950.01.T01:CDS
GTGGGAGATAAAGTGCAAAA+AGG 0.511435 6:+12069831 MsG0680030950.01.T01:CDS
CTACGAGATTATAAGGTTGA+AGG 0.517596 6:-12066800 None:intergenic
ATAAGCATCTGCCGATGGCG+GGG 0.522509 6:-12069249 None:intergenic
GTTAGATTTCAATCCCAAAA+GGG 0.522887 6:-12066970 None:intergenic
GCCATCGGACATTTCAGCGG+TGG 0.524841 6:-12069344 None:intergenic
CCGCTGAAATGTCCGATGGC+AGG 0.527637 6:+12069347 MsG0680030950.01.T01:CDS
GGTGGCGGCGAAGGAAGTGT+CGG 0.528417 6:-12069227 None:intergenic
TGTGGATTCAAGAGATATTG+TGG 0.535318 6:+12069097 MsG0680030950.01.T01:CDS
CCATCGGACATTTCAGCGGT+GGG 0.536910 6:-12069343 None:intergenic
GAAACTGATGAATGAAATTG+AGG 0.538664 6:+12069881 MsG0680030950.01.T01:CDS
TGCTGACTGCGGCATAGCCG+TGG 0.539462 6:-12069189 None:intergenic
CTAGATAAATTAAGAGGAGG+AGG 0.539690 6:-12070566 None:intergenic
AAGGCAGGTGTTGCTTTCAA+AGG 0.540647 6:-12069290 None:intergenic
GTGATTAATCTTACATTACA+AGG 0.544381 6:-12068616 None:intergenic
GTCTCACTTGTTGGATAGCG+TGG 0.544781 6:+12070451 MsG0680030950.01.T01:three_prime_UTR
AAAGCCGCAGCATATAAGAA+TGG 0.545225 6:+12070605 MsG0680030950.01.T01:three_prime_UTR
CTGTAAAAGGTTCCTGCCAT+CGG 0.545550 6:-12069359 None:intergenic
TGGCGAACCTCCATTTGTCC+AGG 0.551244 6:+12069394 MsG0680030950.01.T01:CDS
AATAGTTCCATGAGTACCTA+AGG 0.558063 6:+12070404 MsG0680030950.01.T01:exon
ATGGAGGTTCGCCAGGTCCA+GGG 0.559545 6:-12069385 None:intergenic
GAGGAGGTGAAGACCTAAGA+AGG 0.560143 6:-12070550 None:intergenic
GCATAAGCATCTGCCGATGG+CGG 0.563766 6:-12069251 None:intergenic
TGCGGCATAGCCGTGGCTGG+TGG 0.566443 6:-12069182 None:intergenic
ATATCCATTCTTATATGCTG+CGG 0.582371 6:-12070609 None:intergenic
GCAGGTGTTGCTTTCAAAGG+TGG 0.583909 6:-12069287 None:intergenic
GGAGCATAAGCATCTGCCGA+TGG 0.589113 6:-12069254 None:intergenic
TTTGCAGCCTCCACCAGCCA+CGG 0.589147 6:+12069172 MsG0680030950.01.T01:CDS
GGAACAGCACATTTGGGGCA+TGG 0.591789 6:-12066942 None:intergenic
AAAACCAGTCAGTGTAGACT+TGG 0.593822 6:+12070081 MsG0680030950.01.T01:CDS
CGTACCAAGTCTACACTGAC+TGG 0.595780 6:-12070085 None:intergenic
GCTGAGATACTAGTTGAGGA+TGG 0.596071 6:+12070058 MsG0680030950.01.T01:CDS
GGAAGTGTCGGGTAGTACAT+TGG 0.596331 6:-12069215 None:intergenic
ACCTAATGTTGCTTCACCAA+AGG 0.608619 6:+12068502 MsG0680030950.01.T01:CDS
TACATTGGTGGTGCTGACTG+CGG 0.631260 6:-12069200 None:intergenic
CCTGCCATCGGACATTTCAG+CGG 0.634633 6:-12069347 None:intergenic
TCTCTAGATAAATTAAGAGG+AGG 0.637110 6:-12070569 None:intergenic
AGTGTCGGGTAGTACATTGG+TGG 0.641200 6:-12069212 None:intergenic
AATAGCTGAGATACTAGTTG+AGG 0.649065 6:+12070054 MsG0680030950.01.T01:CDS
TCACATACCTGGACAAATGG+AGG 0.651456 6:-12069401 None:intergenic
AAGAAGGAACAGCACATTTG+GGG 0.668203 6:-12066947 None:intergenic
CCCACCGCTGAAATGTCCGA+TGG 0.677903 6:+12069343 MsG0680030950.01.T01:CDS
CAAAATGGGAGTGTCTCTGG+TGG 0.691345 6:+12068536 MsG0680030950.01.T01:CDS
GGTTCAAGCACAACTTAACG+AGG 0.719957 6:+12068317 MsG0680030950.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAATAAGTAGTATATT+TGG - Chr6:12069546-12069565 None:intergenic 10.0%
!!! TAATTTTCTCAAATTATAAT+CGG - Chr6:12067257-12067276 None:intergenic 10.0%
!! AAACTAATAGTAATTTGAAA+AGG - Chr6:12069511-12069530 None:intergenic 15.0%
!! AAATAAAATGATGAATAGAA+TGG + Chr6:12069788-12069807 MsG0680030950.01.T01:intron 15.0%
!! AAATAATGAAAATTGTTATG+TGG - Chr6:12066828-12066847 None:intergenic 15.0%
!! AACTAATAGTAATTTGAAAA+GGG - Chr6:12069510-12069529 None:intergenic 15.0%
!! AATAATGAAAATTGTTATGT+GGG - Chr6:12066827-12066846 None:intergenic 15.0%
!! AATTAAATCCTAAATAACAT+TGG - Chr6:12068874-12068893 None:intergenic 15.0%
!! ACATAATTCAATGTAATTAT+AGG + Chr6:12067276-12067295 MsG0680030950.01.T01:intron 15.0%
!! ATTAATTGAATTAAATAACC+AGG - Chr6:12068238-12068257 None:intergenic 15.0%
!! GAATTTATATGAATTTGATT+TGG + Chr6:12067095-12067114 MsG0680030950.01.T01:intron 15.0%
!! GACAAAATAACTAAATAAAA+CGG - Chr6:12068667-12068686 None:intergenic 15.0%
!! TAGAAGAAAACATTAATTAA+TGG - Chr6:12070671-12070690 None:intergenic 15.0%
!! TATTTACTATTATATTTCCA+TGG + Chr6:12069067-12069086 MsG0680030950.01.T01:intron 15.0%
!! TTAAAAATCACATATATGAT+TGG + Chr6:12068986-12069005 MsG0680030950.01.T01:intron 15.0%
!!! AAATTAGAATTTTCAGTTAT+TGG - Chr6:12069986-12070005 None:intergenic 15.0%
!!! AAATTTTGAAATAATCACAT+TGG - Chr6:12067497-12067516 None:intergenic 15.0%
!!! AATGTTATTTAGGATTTAAT+TGG + Chr6:12068873-12068892 MsG0680030950.01.T01:intron 15.0%
!!! AGTTAAATCTGAAATAAAAA+AGG - Chr6:12068712-12068731 None:intergenic 15.0%
!!! TAATATTGTTAATTTGTCTT+AGG + Chr6:12069560-12069579 MsG0680030950.01.T01:intron 15.0%
!!! TAGTTGAGATTTATAATTTT+TGG + Chr6:12068786-12068805 MsG0680030950.01.T01:intron 15.0%
!! ACTAATAGTAATTTGAAAAG+GGG - Chr6:12069509-12069528 None:intergenic 20.0%
!! ATAAATAGTAATGTGTCAAA+AGG + Chr6:12068189-12068208 MsG0680030950.01.T01:intron 20.0%
!! ATATCTCTAGATAAATTAAG+AGG - Chr6:12070575-12070594 None:intergenic 20.0%
!! ATATTTGGATTATCTACATT+TGG - Chr6:12070288-12070307 None:intergenic 20.0%
!! GTTATCTATAAAAAAACCTT+TGG + Chr6:12068136-12068155 MsG0680030950.01.T01:intron 20.0%
!! TATTTGGATTATCTACATTT+GGG - Chr6:12070287-12070306 None:intergenic 20.0%
!! TCAATAAATTTCTGATTAAC+AGG - Chr6:12070496-12070515 None:intergenic 20.0%
!! TGTAGAATATATATATGCAA+GGG + Chr6:12067364-12067383 MsG0680030950.01.T01:intron 20.0%
!! TTAAAACGTCTATATACAAA+TGG + Chr6:12069599-12069618 MsG0680030950.01.T01:intron 20.0%
!! TTGTAGAATATATATATGCA+AGG + Chr6:12067363-12067382 MsG0680030950.01.T01:intron 20.0%
!!! AACTGAAAATTCTAATTTGT+TGG + Chr6:12069988-12070007 MsG0680030950.01.T01:intron 20.0%
!!! AAGAAAGTGTAGTTTTTTAT+TGG + Chr6:12066882-12066901 MsG0680030950.01.T01:five_prime_UTR 20.0%
!!! TTGATGTGACTAATTTTTTA+AGG + Chr6:12067850-12067869 MsG0680030950.01.T01:intron 20.0%
! ACAGAAATAGTACTATTACA+AGG + Chr6:12069718-12069737 MsG0680030950.01.T01:intron 25.0%
! ACTAAATTGACAGATTGAAA+AGG + Chr6:12067470-12067489 MsG0680030950.01.T01:intron 25.0%
! AGGATAATAATAGAAGCTAA+GGG + Chr6:12067631-12067650 MsG0680030950.01.T01:intron 25.0%
! CAGAAATAGTACTATTACAA+GGG + Chr6:12069719-12069738 MsG0680030950.01.T01:intron 25.0%
! GTGATTAATCTTACATTACA+AGG - Chr6:12068619-12068638 None:intergenic 25.0%
! TAAAAAATCATTGCCTTCTT+AGG + Chr6:12070537-12070556 MsG0680030950.01.T01:three_prime_UTR 25.0%
! TAGGATAATAATAGAAGCTA+AGG + Chr6:12067630-12067649 MsG0680030950.01.T01:intron 25.0%
! TGTCCATCAATTATAATTGT+CGG + Chr6:12068928-12068947 MsG0680030950.01.T01:intron 25.0%
! TTCAATGTAATTATAGGTGT+CGG + Chr6:12067282-12067301 MsG0680030950.01.T01:intron 25.0%
! TTGAAGAATTCTCAATCATT+TGG + Chr6:12067020-12067039 MsG0680030950.01.T01:CDS 25.0%
! TTGAATCCAAAATACAAAGT+TGG + Chr6:12070218-12070237 MsG0680030950.01.T01:intron 25.0%
! TTGGATTCAAATTACAAAGT+CGG - Chr6:12070208-12070227 None:intergenic 25.0%
!! AAATCTGAAACAAATCAACA+TGG - Chr6:12067132-12067151 None:intergenic 25.0%
!! ATCTGTTTTGTACATATCAT+AGG + Chr6:12068451-12068470 MsG0680030950.01.T01:intron 25.0%
!! TTCTTGCATGTAATTTTCAT+AGG + Chr6:12070711-12070730 MsG0680030950.01.T01:three_prime_UTR 25.0%
!!! AAAGTGTAGTTTTTTATTGG+TGG + Chr6:12066885-12066904 MsG0680030950.01.T01:five_prime_UTR 25.0%
!!! AAGTGTAGTTTTTTATTGGT+GGG + Chr6:12066886-12066905 MsG0680030950.01.T01:five_prime_UTR 25.0%
!!! ATACATGTGAAAAGTTTTAG+AGG + Chr6:12069680-12069699 MsG0680030950.01.T01:intron 25.0%
!!! TGTAAATAAAAGTGTCACAT+TGG + Chr6:12068841-12068860 MsG0680030950.01.T01:intron 25.0%
!!! TGTTGATTTGTTTCAGATTT+TGG + Chr6:12067132-12067151 MsG0680030950.01.T01:intron 25.0%
AAACAAAGATATCAGCAACA+AGG - Chr6:12067757-12067776 None:intergenic 30.0%
AAGGAGAAAGAAAACCAAAA+TGG + Chr6:12068521-12068540 MsG0680030950.01.T01:CDS 30.0%
AAGTTGTGTGTTATATTTCC+TGG + Chr6:12068217-12068236 MsG0680030950.01.T01:intron 30.0%
AATTTGGATTCTATATGGTC+AGG + Chr6:12070159-12070178 MsG0680030950.01.T01:intron 30.0%
ACAGCTGAATTGTAGAAATT+TGG + Chr6:12067728-12067747 MsG0680030950.01.T01:intron 30.0%
AGGAGAAAGAAAACCAAAAT+GGG + Chr6:12068522-12068541 MsG0680030950.01.T01:CDS 30.0%
ATAGTACTATTACAAGGGAA+AGG + Chr6:12069724-12069743 MsG0680030950.01.T01:intron 30.0%
ATATCCATTCTTATATGCTG+CGG - Chr6:12070612-12070631 None:intergenic 30.0%
ATCCAAAATACAAAGTTGGA+AGG + Chr6:12070222-12070241 MsG0680030950.01.T01:intron 30.0%
ATCCTAAATAACATTGGTGT+AGG - Chr6:12068868-12068887 None:intergenic 30.0%
ATTAGTTAGAGAATTGGCAA+AGG - Chr6:12070354-12070373 None:intergenic 30.0%
ATTTGGATTCTATATGGTCA+GGG + Chr6:12070160-12070179 MsG0680030950.01.T01:intron 30.0%
GAAACTGATGAATGAAATTG+AGG + Chr6:12069881-12069900 MsG0680030950.01.T01:CDS 30.0%
GATCCGACAATTATAATTGA+TGG - Chr6:12068934-12068953 None:intergenic 30.0%
GTTAGATTTCAATCCCAAAA+GGG - Chr6:12066973-12066992 None:intergenic 30.0%
TCTCTAGATAAATTAAGAGG+AGG - Chr6:12070572-12070591 None:intergenic 30.0%
TGTTAGATTTCAATCCCAAA+AGG - Chr6:12066974-12066993 None:intergenic 30.0%
TTGAAAAGGGGTTCATATTT+AGG - Chr6:12069497-12069516 None:intergenic 30.0%
! AACAAGATCTTGTTTCAAAG+TGG + Chr6:12066990-12067009 MsG0680030950.01.T01:CDS 30.0%
! ACAAGATCTTGTTTCAAAGT+GGG + Chr6:12066991-12067010 MsG0680030950.01.T01:CDS 30.0%
! TCAAGTTGTTTGCATTATTG+AGG + Chr6:12069854-12069873 MsG0680030950.01.T01:CDS 30.0%
! TTCTTTCTTTTCAGCTCAAA+GGG - Chr6:12066867-12066886 None:intergenic 30.0%
! TTTCTTTCTTTTCAGCTCAA+AGG - Chr6:12066868-12066887 None:intergenic 30.0%
! TTTTACCACATTGAAACTGA+TGG - Chr6:12068097-12068116 None:intergenic 30.0%
!! AAAAACCTTTGGTTTGTAGA+TGG + Chr6:12068147-12068166 MsG0680030950.01.T01:intron 30.0%
!! AAGTGGATTAGTTAGAGAAT+TGG - Chr6:12070360-12070379 None:intergenic 30.0%
!!! AAAAGTTTTAGAGGAGCTTA+CGG + Chr6:12069689-12069708 MsG0680030950.01.T01:intron 30.0%
!!! AGTGTAGTTTTTTATTGGTG+GGG + Chr6:12066887-12066906 MsG0680030950.01.T01:five_prime_UTR 30.0%
!!! TGAGAATGATGCCATTTTTT+TGG - Chr6:12066919-12066938 None:intergenic 30.0%
!!! TTTTGGTTTTCTTTCTCCTT+TGG - Chr6:12068521-12068540 None:intergenic 30.0%
AATAGCTGAGATACTAGTTG+AGG + Chr6:12070054-12070073 MsG0680030950.01.T01:CDS 35.0%
AATAGTTCCATGAGTACCTA+AGG + Chr6:12070404-12070423 MsG0680030950.01.T01:exon 35.0%
AATGTCTATGTTCACATACC+TGG - Chr6:12069415-12069434 None:intergenic 35.0%
AGAAACCATCTACAAACCAA+AGG - Chr6:12068155-12068174 None:intergenic 35.0%
AGAGAGACTGATATGATTCA+AGG - Chr6:12067435-12067454 None:intergenic 35.0%
ATACACCATCAGTTTCAATG+TGG + Chr6:12068089-12068108 MsG0680030950.01.T01:intron 35.0%
ATCTTCTCTGACTATTTCAG+TGG + Chr6:12067922-12067941 MsG0680030950.01.T01:intron 35.0%
CAAATTACAAAGTCGGAAGT+TGG - Chr6:12070201-12070220 None:intergenic 35.0%
CAACTTATGAAGATACTCCT+CGG - Chr6:12067346-12067365 None:intergenic 35.0%
CAAGAAACAATTTCCTGTCA+AGG + Chr6:12068296-12068315 MsG0680030950.01.T01:CDS 35.0%
CATGGAACTATTACGAAAAG+AGG - Chr6:12070396-12070415 None:intergenic 35.0%
CTACGAGATTATAAGGTTGA+AGG - Chr6:12066803-12066822 None:intergenic 35.0%
CTAGATAAATTAAGAGGAGG+AGG - Chr6:12070569-12070588 None:intergenic 35.0%
CTGCGACAAAAGCAATAAAA+AGG - Chr6:12070017-12070036 None:intergenic 35.0%
TAGTCCTGAAAATGTAAGCA+TGG - Chr6:12067545-12067564 None:intergenic 35.0%
TGTGGATTCAAGAGATATTG+TGG + Chr6:12069097-12069116 MsG0680030950.01.T01:CDS 35.0%
! ACATTGAGCTTTATGCTGAA+TGG + Chr6:12068374-12068393 MsG0680030950.01.T01:intron 35.0%
! CACCTTCCAACTTTGTATTT+TGG - Chr6:12070227-12070246 None:intergenic 35.0%
! GACCTACACCAATGTTATTT+AGG + Chr6:12068863-12068882 MsG0680030950.01.T01:intron 35.0%
! GTTTTCTTCTATGAACCTGT+TGG + Chr6:12070680-12070699 MsG0680030950.01.T01:three_prime_UTR 35.0%
! TGCTGAATGGAAATTTTACG+CGG + Chr6:12068387-12068406 MsG0680030950.01.T01:intron 35.0%
! TTCAGCTTCATTTGGAATTC+AGG + Chr6:12067058-12067077 MsG0680030950.01.T01:CDS 35.0%
! TTCTTAGTCTAAGACTAAGC+TGG - Chr6:12067211-12067230 None:intergenic 35.0%
!! AAAACTGAGTATTGACTGCT+CGG - Chr6:12069450-12069469 None:intergenic 35.0%
!! ACTTTGAAATGAGGTTCCTT+AGG - Chr6:12070423-12070442 None:intergenic 35.0%
!! CTTTTTATTGCTTTTGTCGC+AGG + Chr6:12070015-12070034 MsG0680030950.01.T01:intron 35.0%
!! TGAGATGATGCACTGTTTTT+GGG - Chr6:12069323-12069342 None:intergenic 35.0%
!!! TAGTTTTTTATTGGTGGGGT+TGG + Chr6:12066891-12066910 MsG0680030950.01.T01:five_prime_UTR 35.0%
AAAACCAGTCAGTGTAGACT+TGG + Chr6:12070081-12070100 MsG0680030950.01.T01:CDS 40.0%
AAAGCCGCAGCATATAAGAA+TGG + Chr6:12070605-12070624 MsG0680030950.01.T01:three_prime_UTR 40.0%
AAGAAGGAACAGCACATTTG+GGG - Chr6:12066950-12066969 None:intergenic 40.0%
CGCGCAATTTGGATTCTATA+TGG + Chr6:12070154-12070173 MsG0680030950.01.T01:intron 40.0%
GAAGAAGGAACAGCACATTT+GGG - Chr6:12066951-12066970 None:intergenic 40.0%
GCAAGAACATAACATCCAAC+AGG - Chr6:12070698-12070717 None:intergenic 40.0%
GGGTTCATATTTAGGCACAA+AGG - Chr6:12069489-12069508 None:intergenic 40.0%
GTGGGAGATAAAGTGCAAAA+AGG + Chr6:12069831-12069850 MsG0680030950.01.T01:CDS 40.0%
TATTTCCATGGCTAGACTTG+TGG + Chr6:12069079-12069098 MsG0680030950.01.T01:intron 40.0%
TCCACTATGCGAATTACACA+AGG - Chr6:12067900-12067919 None:intergenic 40.0%
TCCTTTGGTGAAGCAACATT+AGG - Chr6:12068506-12068525 None:intergenic 40.0%
TGTGGGTCTACGAGATTATA+AGG - Chr6:12066810-12066829 None:intergenic 40.0%
TGTTCACATACCTGGACAAA+TGG - Chr6:12069407-12069426 None:intergenic 40.0%
TTCAATCCCAAAAGGGAAGA+AGG - Chr6:12066966-12066985 None:intergenic 40.0%
! AAGGGAAAGGACTTTTGTTC+TGG + Chr6:12069737-12069756 MsG0680030950.01.T01:intron 40.0%
! ACCTAATGTTGCTTCACCAA+AGG + Chr6:12068502-12068521 MsG0680030950.01.T01:CDS 40.0%
! GTGAGATGATGCACTGTTTT+TGG - Chr6:12069324-12069343 None:intergenic 40.0%
! GTGTCCATGCTTACATTTTC+AGG + Chr6:12067538-12067557 MsG0680030950.01.T01:intron 40.0%
!! CTCGTTTCGACTTTGAAATG+AGG - Chr6:12070432-12070451 None:intergenic 40.0%
!! GTAATTATAGGTGTCGGTGT+CGG + Chr6:12067288-12067307 MsG0680030950.01.T01:intron 40.0%
!! TCTGCTGATTCAGCTTCATT+TGG + Chr6:12067050-12067069 MsG0680030950.01.T01:CDS 40.0%
!! TGGGGTTGGTTCCAAAAAAA+TGG + Chr6:12066905-12066924 MsG0680030950.01.T01:exon 40.0%
!!! AGATGATGCACTGTTTTTGG+GGG - Chr6:12069321-12069340 None:intergenic 40.0%
!!! GAGATGATGCACTGTTTTTG+GGG - Chr6:12069322-12069341 None:intergenic 40.0%
AGTTGTGCTTGAACCTTGAC+AGG - Chr6:12068312-12068331 None:intergenic 45.0%
CTGTAAAAGGTTCCTGCCAT+CGG - Chr6:12069362-12069381 None:intergenic 45.0%
GAACCTGTGCAACGTATAGA+GGG + Chr6:12068740-12068759 MsG0680030950.01.T01:intron 45.0%
GAAGATACTCCTCGGATAAG+AGG - Chr6:12067338-12067357 None:intergenic 45.0%
GCCTTGTGTAATTCGCATAG+TGG + Chr6:12067896-12067915 MsG0680030950.01.T01:intron 45.0%
GCTGAGATACTAGTTGAGGA+TGG + Chr6:12070058-12070077 MsG0680030950.01.T01:CDS 45.0%
GGAAGAAGGAACAGCACATT+TGG - Chr6:12066952-12066971 None:intergenic 45.0%
GGTTCAAGCACAACTTAACG+AGG + Chr6:12068317-12068336 MsG0680030950.01.T01:CDS 45.0%
TCACATACCTGGACAAATGG+AGG - Chr6:12069404-12069423 None:intergenic 45.0%
TCGAAACGAGTCTCACTTGT+TGG + Chr6:12070442-12070461 MsG0680030950.01.T01:three_prime_UTR 45.0%
TGAACCTGTGCAACGTATAG+AGG + Chr6:12068739-12068758 MsG0680030950.01.T01:intron 45.0%
TGAATCCACAAGTCTAGCCA+TGG - Chr6:12069087-12069106 None:intergenic 45.0%
TGAGGTTCCTTAGGTACTCA+TGG - Chr6:12070414-12070433 None:intergenic 45.0%
TTTCCCTCTATACGTTGCAC+AGG - Chr6:12068746-12068765 None:intergenic 45.0%
! ATTTTTCTGTCGCTGCAGGT+GGG + Chr6:12069813-12069832 MsG0680030950.01.T01:intron 45.0%
! GCTGTTCCTTCTTCCCTTTT+GGG + Chr6:12066957-12066976 MsG0680030950.01.T01:CDS 45.0%
! TATTTTTCTGTCGCTGCAGG+TGG + Chr6:12069812-12069831 MsG0680030950.01.T01:intron 45.0%
! TGCTGTTCCTTCTTCCCTTT+TGG + Chr6:12066956-12066975 MsG0680030950.01.T01:CDS 45.0%
!! AACCAAAATGGGAGTGTCTC+TGG + Chr6:12068533-12068552 MsG0680030950.01.T01:CDS 45.0%
!! AAGGCAGGTGTTGCTTTCAA+AGG - Chr6:12069293-12069312 None:intergenic 45.0%
!!! GATGATGCACTGTTTTTGGG+GGG - Chr6:12069320-12069339 None:intergenic 45.0%
!!! GGTTATTTTTCTGTCGCTGC+AGG + Chr6:12069809-12069828 MsG0680030950.01.T01:intron 45.0%
!! TTAATTAATATATTAATCAT+AGG + Chr6:12067611-12067630 MsG0680030950.01.T01:intron 5.0%
ATGTGTGTGTGCGCGCAATT+TGG + Chr6:12070143-12070162 MsG0680030950.01.T01:intron 50.0%
CGTACCAAGTCTACACTGAC+TGG - Chr6:12070088-12070107 None:intergenic 50.0%
CTCGCGCACACACACATATT+TGG - Chr6:12070303-12070322 None:intergenic 50.0%
GAGGAGGTGAAGACCTAAGA+AGG - Chr6:12070553-12070572 None:intergenic 50.0%
GAGGCTGCATTGAGTACAAG+TGG - Chr6:12070377-12070396 None:intergenic 50.0%
TACTCCTCGGATAAGAGGTG+TGG - Chr6:12067333-12067352 None:intergenic 50.0%
TTCAAAGGTGGCGAGCTTGT+AGG - Chr6:12069278-12069297 None:intergenic 50.0%
TTGTCGCAGGCTGATCAATC+AGG + Chr6:12070028-12070047 MsG0680030950.01.T01:intron 50.0%
! AGTGTCGGGTAGTACATTGG+TGG - Chr6:12069215-12069234 None:intergenic 50.0%
! CACCAGAGACACTCCCATTT+TGG - Chr6:12068538-12068557 None:intergenic 50.0%
! CGATGGCAGGAACCTTTTAC+AGG + Chr6:12069360-12069379 MsG0680030950.01.T01:CDS 50.0%
! GTCTCACTTGTTGGATAGCG+TGG + Chr6:12070451-12070470 MsG0680030950.01.T01:three_prime_UTR 50.0%
!! CAAAATGGGAGTGTCTCTGG+TGG + Chr6:12068536-12068555 MsG0680030950.01.T01:CDS 50.0%
!! GCAGGTGTTGCTTTCAAAGG+TGG - Chr6:12069290-12069309 None:intergenic 50.0%
!! GGAAGTGTCGGGTAGTACAT+TGG - Chr6:12069218-12069237 None:intergenic 50.0%
!! TACATTGGTGGTGCTGACTG+CGG - Chr6:12069203-12069222 None:intergenic 50.0%
!!! ACTGTTTTTGGGGGGAGGTA+AGG - Chr6:12069312-12069331 None:intergenic 50.0%
ATAAGCATCTGCCGATGGCG+GGG - Chr6:12069252-12069271 None:intergenic 55.0%
CATAAGCATCTGCCGATGGC+GGG - Chr6:12069253-12069272 None:intergenic 55.0%
CCATCGGACATTTCAGCGGT+GGG - Chr6:12069346-12069365 None:intergenic 55.0%
CCTGCCATCGGACATTTCAG+CGG - Chr6:12069350-12069369 None:intergenic 55.0%
GACACCACACCTCTTATCCG+AGG + Chr6:12067326-12067345 MsG0680030950.01.T01:intron 55.0%
GCATAAGCATCTGCCGATGG+CGG - Chr6:12069254-12069273 None:intergenic 55.0%
GGAGCATAAGCATCTGCCGA+TGG - Chr6:12069257-12069276 None:intergenic 55.0%
TGGACAAATGGAGGTTCGCC+AGG - Chr6:12069395-12069414 None:intergenic 55.0%
TGGCGAACCTCCATTTGTCC+AGG + Chr6:12069394-12069413 MsG0680030950.01.T01:CDS 55.0%
TGTACTTGCATGCAGAGCGC+TGG - Chr6:12068641-12068660 None:intergenic 55.0%
! AGGTCCAGGGCACCTGTAAA+AGG - Chr6:12069375-12069394 None:intergenic 55.0%
! GGAACAGCACATTTGGGGCA+TGG - Chr6:12066945-12066964 None:intergenic 55.0%
! GGAACCTTTTACAGGTGCCC+TGG + Chr6:12069368-12069387 MsG0680030950.01.T01:CDS 55.0%
! TTTTACAGGTGCCCTGGACC+TGG + Chr6:12069374-12069393 MsG0680030950.01.T01:CDS 55.0%
!! TTTTTGGGGGGAGGTAAGGC+AGG - Chr6:12069308-12069327 None:intergenic 55.0%
!!! GATGCACTGTTTTTGGGGGG+AGG - Chr6:12069317-12069336 None:intergenic 55.0%
AATGGAGGTTCGCCAGGTCC+AGG - Chr6:12069389-12069408 None:intergenic 60.0%
ATGGAGGTTCGCCAGGTCCA+GGG - Chr6:12069388-12069407 None:intergenic 60.0%
CCCACCGCTGAAATGTCCGA+TGG + Chr6:12069343-12069362 MsG0680030950.01.T01:CDS 60.0%
CCGCTGAAATGTCCGATGGC+AGG + Chr6:12069347-12069366 MsG0680030950.01.T01:CDS 60.0%
GCCATCGGACATTTCAGCGG+TGG - Chr6:12069347-12069366 None:intergenic 60.0%
TTTGCAGCCTCCACCAGCCA+CGG + Chr6:12069172-12069191 MsG0680030950.01.T01:CDS 60.0%
! TAAGCATCTGCCGATGGCGG+GGG - Chr6:12069251-12069270 None:intergenic 60.0%
! GGTGGCGGCGAAGGAAGTGT+CGG - Chr6:12069230-12069249 None:intergenic 65.0%
! TGCTGACTGCGGCATAGCCG+TGG - Chr6:12069192-12069211 None:intergenic 65.0%
!! GGGAGTGTCTCTGGTGGCAC+TGG + Chr6:12068542-12068561 MsG0680030950.01.T01:CDS 65.0%
!! GTGGCGGCGAAGGAAGTGTC+GGG - Chr6:12069229-12069248 None:intergenic 65.0%
GACTGCGGCATAGCCGTGGC+TGG - Chr6:12069188-12069207 None:intergenic 70.0%
GGCATAGCCGTGGCTGGTGG+AGG - Chr6:12069182-12069201 None:intergenic 70.0%
TGCGGCATAGCCGTGGCTGG+TGG - Chr6:12069185-12069204 None:intergenic 70.0%
TTCGCCGCCACCCCCGCCAT+CGG + Chr6:12069238-12069257 MsG0680030950.01.T01:CDS 75.0%
!! GCATCTGCCGATGGCGGGGG+TGG - Chr6:12069248-12069267 None:intergenic 75.0%
!! TCTGCCGATGGCGGGGGTGG+CGG - Chr6:12069245-12069264 None:intergenic 75.0%
!!! GATGGCGGGGGTGGCGGCGA+AGG - Chr6:12069239-12069258 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr6 gene 12066743 12070830 12066743 ID=MsG0680030950.01;Name=MsG0680030950.01
Chr6 mRNA 12066743 12070830 12066743 ID=MsG0680030950.01.T01;Parent=MsG0680030950.01;Name=MsG0680030950.01.T01;_AED=0.41;_eAED=0.41;_QI=181|1|1|1|0.83|0.71|7|414|280
Chr6 exon 12066743 12067079 12066743 ID=MsG0680030950.01.T01:exon:8687;Parent=MsG0680030950.01.T01
Chr6 exon 12068288 12068338 12068288 ID=MsG0680030950.01.T01:exon:8688;Parent=MsG0680030950.01.T01
Chr6 exon 12068473 12068624 12068473 ID=MsG0680030950.01.T01:exon:8689;Parent=MsG0680030950.01.T01
Chr6 exon 12069094 12069415 12069094 ID=MsG0680030950.01.T01:exon:8690;Parent=MsG0680030950.01.T01
Chr6 exon 12069831 12069902 12069831 ID=MsG0680030950.01.T01:exon:8691;Parent=MsG0680030950.01.T01
Chr6 exon 12070037 12070102 12070037 ID=MsG0680030950.01.T01:exon:8692;Parent=MsG0680030950.01.T01
Chr6 exon 12070393 12070830 12070393 ID=MsG0680030950.01.T01:exon:8693;Parent=MsG0680030950.01.T01
Chr6 five_prime_UTR 12066743 12066923 12066743 ID=MsG0680030950.01.T01:five_prime_utr;Parent=MsG0680030950.01.T01
Chr6 CDS 12066924 12067079 12066924 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 CDS 12068288 12068338 12068288 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 CDS 12068473 12068624 12068473 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 CDS 12069094 12069415 12069094 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 CDS 12069831 12069902 12069831 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 CDS 12070037 12070102 12070037 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 CDS 12070393 12070416 12070393 ID=MsG0680030950.01.T01:cds;Parent=MsG0680030950.01.T01
Chr6 three_prime_UTR 12070417 12070830 12070417 ID=MsG0680030950.01.T01:three_prime_utr;Parent=MsG0680030950.01.T01
Gene Sequence

>MsG0680030950.01.T01

ATGGCATCATTCTCAGTTCCATGCCCCAAATGTGCTGTTCCTTCTTCCCTTTTGGGATTGAAATCTAACAAGATCTTGTTTCAAAGTGGGTTGAGTTTGAAGAATTCTCAATCATTTGGATCTTTGTCTGCTGATTCAGCTTCATTTGGAATTCAGTGTCTTAACAAGAAACAATTTCCTGTCAAGGTTCAAGCACAACTTAACGAGGCTGCTGTTGTCGAAAATTCGAACTCTGCACCTAATGTTGCTTCACCAAAGGAGAAAGAAAACCAAAATGGGAGTGTCTCTGGTGGCACTGGCACTGTGTCAGATGAATCTGCAGTTTCTGCGTTCATGTCTCAAGTTGCTGACCTTGTAATACTTGTGGATTCAAGAGATATTGTGGAGCTGCAACTAAAGCAATCAGATTATGAACTTGTGATAAGAAAAAAAGAAGCTTTGCAGCCTCCACCAGCCACGGCTATGCCGCAGTCAGCACCACCAATGTACTACCCGACACTTCCTTCGCCGCCACCCCCGCCATCGGCAGATGCTTATGCTCCTACAAGCTCGCCACCTTTGAAAGCAACACCTGCCTTACCTCCCCCCAAAAACAGTGCATCATCTCACCCACCGCTGAAATGTCCGATGGCAGGAACCTTTTACAGGTGCCCTGGACCTGGCGAACCTCCATTTGTCCAGGTGGGAGATAAAGTGCAAAAAGGTCAAGTTGTTTGCATTATTGAGGCTATGAAACTGATGAATGAAATTGAGGCTGATCAATCAGGAACAATAGCTGAGATACTAGTTGAGGATGGAAAACCAGTCAGTGTAGACTTGCCTCTTTTCGTAATAGTTCCATGA
Protein sequence

>MsG0680030950.01.T01

MASFSVPCPKCAVPSSLLGLKSNKILFQSGLSLKNSQSFGSLSADSASFGIQCLNKKQFPVKVQAQLNEAAVVENSNSAPNVASPKEKENQNGSVSGGTGTVSDESAVSAFMSQVADLVILVDSRDIVELQLKQSDYELVIRKKEALQPPPATAMPQSAPPMYYPTLPSPPPPPSADAYAPTSSPPLKATPALPPPKNSASSHPPLKCPMAGTFYRCPGPGEPPFVQVGDKVQKGQVVCIIEAMKLMNEIEADQSGTIAEILVEDGKPVSVDLPLFVIVP*