Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032105.01.T01 | XP_039686428.1 | 90.123 | 81 | 8 | 0 | 1 | 81 | 613 | 693 | 8.70E-41 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032105.01.T01 | A0A072V841 | 90.123 | 81 | 8 | 0 | 1 | 81 | 924 | 1004 | 7.22e-41 | 152 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032093.01 | MsG0680032105.01 | 0.838501 | 2.769742e-57 | 3.716854e-54 |
| MsG0680032100.01 | MsG0680032105.01 | 0.899249 | 2.389284e-77 | 2.929783e-73 |
| MsG0680032101.01 | MsG0680032105.01 | 0.881783 | 1.801520e-70 | 1.069496e-66 |
| MsG0680032103.01 | MsG0680032105.01 | 0.899503 | 1.857811e-77 | 2.304223e-73 |
| MsG0680032105.01 | MsG0680032108.01 | 0.871348 | 7.317987e-67 | 2.942463e-63 |
| MsG0680032105.01 | MsG0680032109.01 | 0.841932 | 3.518939e-58 | 5.251058e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032105.01.T01 | MTR_2g040910 | 90.123 | 81 | 8 | 0 | 1 | 81 | 924 | 1004 | 1.83e-44 | 152 |
| MsG0680032105.01.T01 | MTR_6g036890 | 90.123 | 81 | 8 | 0 | 1 | 81 | 889 | 969 | 2.17e-44 | 152 |
| MsG0680032105.01.T01 | MTR_6g036840 | 87.654 | 81 | 10 | 0 | 1 | 81 | 924 | 1004 | 1.50e-43 | 149 |
| MsG0680032105.01.T01 | MTR_6g036790 | 88.889 | 81 | 9 | 0 | 1 | 81 | 899 | 979 | 4.59e-43 | 148 |
| MsG0680032105.01.T01 | MTR_6g036780 | 89.024 | 82 | 8 | 1 | 1 | 81 | 886 | 967 | 7.30e-43 | 147 |
| MsG0680032105.01.T01 | MTR_5g026160 | 77.778 | 81 | 15 | 1 | 1 | 81 | 895 | 972 | 2.24e-36 | 129 |
| MsG0680032105.01.T01 | MTR_5g025880 | 74.074 | 81 | 21 | 0 | 1 | 81 | 1200 | 1280 | 3.16e-34 | 123 |
| MsG0680032105.01.T01 | MTR_5g025860 | 72.840 | 81 | 22 | 0 | 1 | 81 | 892 | 972 | 9.15e-34 | 121 |
| MsG0680032105.01.T01 | MTR_5g025930 | 71.605 | 81 | 23 | 0 | 1 | 81 | 895 | 975 | 1.48e-33 | 121 |
| MsG0680032105.01.T01 | MTR_5g024420 | 66.250 | 80 | 27 | 0 | 1 | 80 | 11 | 90 | 4.48e-33 | 110 |
| MsG0680032105.01.T01 | MTR_5g026150 | 69.136 | 81 | 25 | 0 | 1 | 81 | 893 | 973 | 1.43e-32 | 118 |
| MsG0680032105.01.T01 | MTR_5g025950 | 69.136 | 81 | 25 | 0 | 1 | 81 | 924 | 1004 | 4.34e-32 | 117 |
| MsG0680032105.01.T01 | MTR_5g026090 | 73.418 | 79 | 15 | 2 | 1 | 78 | 857 | 930 | 5.98e-32 | 116 |
| MsG0680032105.01.T01 | MTR_5g026010 | 66.667 | 81 | 27 | 0 | 1 | 81 | 911 | 991 | 1.59e-31 | 115 |
| MsG0680032105.01.T01 | MTR_5g026200 | 67.901 | 81 | 26 | 0 | 1 | 81 | 767 | 847 | 1.23e-30 | 112 |
| MsG0680032105.01.T01 | MTR_5g044680 | 61.728 | 81 | 31 | 0 | 1 | 81 | 827 | 907 | 3.71e-30 | 111 |
| MsG0680032105.01.T01 | MTR_5g044680 | 61.728 | 81 | 31 | 0 | 1 | 81 | 923 | 1003 | 3.94e-30 | 111 |
| MsG0680032105.01.T01 | MTR_6g036870 | 80.597 | 67 | 8 | 1 | 1 | 67 | 864 | 925 | 7.91e-30 | 110 |
| MsG0680032105.01.T01 | MTR_5g026000 | 69.136 | 81 | 25 | 0 | 1 | 81 | 911 | 991 | 1.76e-29 | 109 |
| MsG0680032105.01.T01 | MTR_5g024450 | 65.432 | 81 | 28 | 0 | 1 | 81 | 929 | 1009 | 2.75e-29 | 108 |
| MsG0680032105.01.T01 | MTR_5g025890 | 66.667 | 81 | 21 | 1 | 1 | 81 | 920 | 994 | 1.24e-28 | 107 |
| MsG0680032105.01.T01 | MTR_5g019070 | 62.963 | 81 | 30 | 0 | 1 | 81 | 912 | 992 | 1.38e-28 | 107 |
| MsG0680032105.01.T01 | MTR_5g025850 | 60.494 | 81 | 32 | 0 | 1 | 81 | 905 | 985 | 6.80e-28 | 105 |
| MsG0680032105.01.T01 | MTR_5g025840 | 53.086 | 81 | 37 | 1 | 1 | 81 | 880 | 959 | 1.50e-22 | 89.7 |
| MsG0680032105.01.T01 | MTR_1g088935 | 50.000 | 82 | 40 | 1 | 1 | 81 | 740 | 821 | 1.06e-21 | 87.4 |
| MsG0680032105.01.T01 | MTR_8g068540 | 48.780 | 82 | 41 | 1 | 1 | 81 | 919 | 1000 | 4.29e-20 | 82.8 |
| MsG0680032105.01.T01 | MTR_3g070220 | 50.617 | 81 | 37 | 1 | 1 | 81 | 913 | 990 | 8.24e-20 | 82.0 |
| MsG0680032105.01.T01 | MTR_1g088940 | 48.315 | 89 | 31 | 2 | 1 | 81 | 915 | 996 | 2.00e-19 | 80.9 |
| MsG0680032105.01.T01 | MTR_1g088940 | 48.315 | 89 | 31 | 2 | 1 | 81 | 958 | 1039 | 2.04e-19 | 80.9 |
| MsG0680032105.01.T01 | MTR_8g071950 | 49.383 | 81 | 40 | 1 | 1 | 80 | 50 | 130 | 5.47e-19 | 75.9 |
| MsG0680032105.01.T01 | MTR_8g066700 | 47.561 | 82 | 33 | 3 | 1 | 81 | 918 | 990 | 2.38e-15 | 69.3 |
| MsG0680032105.01.T01 | MTR_1g088930 | 45.882 | 85 | 40 | 3 | 1 | 81 | 918 | 1000 | 1.30e-14 | 67.4 |
| MsG0680032105.01.T01 | MTR_5g082275 | 41.975 | 81 | 36 | 3 | 1 | 78 | 56 | 128 | 2.00e-13 | 61.6 |
| MsG0680032105.01.T01 | MTR_1g088945 | 63.158 | 38 | 14 | 0 | 1 | 38 | 133 | 170 | 5.48e-13 | 60.8 |
| MsG0680032105.01.T01 | MTR_1g029940 | 41.250 | 80 | 40 | 3 | 1 | 79 | 843 | 916 | 2.07e-11 | 58.2 |
| MsG0680032105.01.T01 | MTR_2g072600 | 40.741 | 81 | 37 | 3 | 1 | 78 | 187 | 259 | 4.27e-11 | 57.0 |
| MsG0680032105.01.T01 | MTR_7g067530 | 34.066 | 91 | 50 | 2 | 1 | 81 | 895 | 985 | 6.88e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAATCTTATAAAGATTT+TGG | 0.115967 | 6:+33700698 | MsG0680032105.01.T01:CDS |
| CACAATATTCATTCTTTCTT+TGG | 0.175993 | 6:-33700849 | None:intergenic |
| CTTATGCTGGAAATGCTTAC+TGG | 0.274178 | 6:+33700606 | None:intergenic |
| AAAATCTTTATAAGATTATC+AGG | 0.288665 | 6:-33700696 | None:intergenic |
| ATTGCTCCTTCTTCAGCTCT+TGG | 0.357284 | 6:-33700735 | None:intergenic |
| GAAGAAGGAGCAATAGAAGA+TGG | 0.511758 | 6:+33700744 | MsG0680032105.01.T01:CDS |
| ACTAAGCACTCCTCTACAGT+TGG | 0.543330 | 6:-33700786 | None:intergenic |
| GATTCTTATTCCAACTGTAG+AGG | 0.606375 | 6:+33700776 | MsG0680032105.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCTTTATAAGATTATC+AGG | - | Chr6:33700699-33700718 | None:intergenic | 15.0% |
| !!! | TGATAATCTTATAAAGATTT+TGG | + | Chr6:33700698-33700717 | MsG0680032105.01.T01:CDS | 15.0% |
| !!! | TTAGTTTCACTTTTTAGGAT+TGG | + | Chr6:33700804-33700823 | MsG0680032105.01.T01:CDS | 25.0% |
| ATCTTCAAAAAGTTCATCAG+TGG | - | Chr6:33700640-33700659 | None:intergenic | 30.0% | |
| TCTTCAAAAAGTTCATCAGT+GGG | - | Chr6:33700639-33700658 | None:intergenic | 30.0% | |
| !! | ACTGATGAACTTTTTGAAGA+TGG | + | Chr6:33700639-33700658 | MsG0680032105.01.T01:CDS | 30.0% |
| !!! | AGTGCTTAGTTTCACTTTTT+AGG | + | Chr6:33700799-33700818 | MsG0680032105.01.T01:CDS | 30.0% |
| !!! | GATTGGACTTTTTTGTTCAT+TGG | + | Chr6:33700821-33700840 | MsG0680032105.01.T01:CDS | 30.0% |
| GATTCTTATTCCAACTGTAG+AGG | + | Chr6:33700776-33700795 | MsG0680032105.01.T01:CDS | 35.0% | |
| GAAGAAGGAGCAATAGAAGA+TGG | + | Chr6:33700744-33700763 | MsG0680032105.01.T01:CDS | 40.0% | |
| ! | CTTTTACCAAGAGCTGAAGA+AGG | + | Chr6:33700729-33700748 | MsG0680032105.01.T01:CDS | 40.0% |
| ACTAAGCACTCCTCTACAGT+TGG | - | Chr6:33700789-33700808 | None:intergenic | 45.0% | |
| ATTGCTCCTTCTTCAGCTCT+TGG | - | Chr6:33700738-33700757 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 33700618 | 33700863 | 33700618 | ID=MsG0680032105.01;Name=MsG0680032105.01 |
| Chr6 | mRNA | 33700618 | 33700863 | 33700618 | ID=MsG0680032105.01.T01;Parent=MsG0680032105.01;Name=MsG0680032105.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|81 |
| Chr6 | exon | 33700618 | 33700863 | 33700618 | ID=MsG0680032105.01.T01:exon:18525;Parent=MsG0680032105.01.T01 |
| Chr6 | CDS | 33700618 | 33700863 | 33700618 | ID=MsG0680032105.01.T01:cds;Parent=MsG0680032105.01.T01 |
| Gene Sequence |
| Protein sequence |
