Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032278.01.T01 | XP_013451894.2 | 73.09 | 1152 | 230 | 5 | 1 | 1075 | 59 | 1207 | 0 | 1604 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032278.01.T01 | A0A396HCS8 | 73.090 | 1152 | 230 | 5 | 1 | 1075 | 59 | 1207 | 0.0 | 1604 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011309.01 | MsG0680032278.01 | 0.803233 | 3.844204e-49 | 1.921611e-46 |
| MsG0380012125.01 | MsG0680032278.01 | 0.802949 | 4.401349e-49 | 2.184159e-46 |
| MsG0480020433.01 | MsG0680032278.01 | 0.823291 | 1.499369e-53 | 1.285621e-50 |
| MsG0680032002.01 | MsG0680032278.01 | 0.808678 | 2.752314e-50 | 1.583531e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032278.01.T01 | MTR_6g037750 | 73.090 | 1152 | 230 | 5 | 1 | 1075 | 56 | 1204 | 0.0 | 1604 |
| MsG0680032278.01.T01 | MTR_6g039180 | 72.256 | 1157 | 210 | 8 | 1 | 1076 | 60 | 1186 | 0.0 | 1583 |
| MsG0680032278.01.T01 | MTR_6g038940 | 76.987 | 1082 | 220 | 7 | 1 | 1075 | 60 | 1119 | 0.0 | 1562 |
| MsG0680032278.01.T01 | MTR_6g038700 | 68.385 | 1183 | 260 | 8 | 1 | 1075 | 59 | 1235 | 0.0 | 1504 |
| MsG0680032278.01.T01 | MTR_6g038670 | 71.095 | 1114 | 233 | 8 | 1 | 1075 | 58 | 1121 | 0.0 | 1502 |
| MsG0680032278.01.T01 | MTR_6g038730 | 69.351 | 1155 | 215 | 8 | 1 | 1075 | 57 | 1152 | 0.0 | 1498 |
| MsG0680032278.01.T01 | MTR_6g038910 | 71.070 | 1075 | 205 | 7 | 54 | 1071 | 45 | 1070 | 0.0 | 1425 |
| MsG0680032278.01.T01 | MTR_6g038910 | 29.138 | 731 | 397 | 34 | 12 | 710 | 331 | 972 | 1.51e-27 | 121 |
| MsG0680032278.01.T01 | MTR_6g039110 | 66.398 | 1116 | 254 | 14 | 1 | 1076 | 54 | 1088 | 0.0 | 1340 |
| MsG0680032278.01.T01 | MTR_6g038790 | 79.618 | 628 | 102 | 3 | 468 | 1071 | 1 | 626 | 0.0 | 959 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032278.01.T01 | AT2G34930 | 34.254 | 905 | 445 | 25 | 143 | 1001 | 100 | 900 | 3.23e-110 | 365 |
| MsG0680032278.01.T01 | AT2G34930 | 28.139 | 892 | 481 | 30 | 2 | 789 | 55 | 890 | 7.62e-53 | 201 |
| MsG0680032278.01.T01 | AT2G15080 | 32.831 | 996 | 527 | 35 | 88 | 1057 | 88 | 967 | 2.25e-94 | 323 |
| MsG0680032278.01.T01 | AT2G15080 | 29.807 | 879 | 484 | 27 | 4 | 807 | 63 | 883 | 2.15e-52 | 200 |
| MsG0680032278.01.T01 | AT2G15080 | 32.831 | 996 | 527 | 35 | 88 | 1057 | 88 | 967 | 2.25e-94 | 323 |
| MsG0680032278.01.T01 | AT2G15080 | 29.807 | 879 | 484 | 27 | 4 | 807 | 63 | 883 | 2.15e-52 | 200 |
| MsG0680032278.01.T01 | AT3G11010 | 31.070 | 972 | 511 | 34 | 112 | 1056 | 37 | 876 | 1.59e-77 | 274 |
| MsG0680032278.01.T01 | AT3G11010 | 29.839 | 868 | 458 | 28 | 4 | 807 | 12 | 792 | 8.18e-58 | 216 |
| MsG0680032278.01.T01 | AT3G11010 | 40.909 | 110 | 60 | 1 | 88 | 192 | 708 | 817 | 4.62e-12 | 70.9 |
| MsG0680032278.01.T01 | AT2G33060 | 33.425 | 724 | 406 | 24 | 333 | 1037 | 87 | 753 | 1.75e-77 | 272 |
Find 197 sgRNAs with CRISPR-Local
Find 221 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTAAGTCTAAGAAGTTG+TGG | 0.538178 | 6:-37744781 | MsG0680032278.01.T01:CDS |
| AAAGTTGAATGCTTTGTAGA+TGG | 0.390264 | 6:+37743153 | None:intergenic |
| AAATAATAAATTGAACAGTG+GGG | 0.677023 | 6:-37744527 | MsG0680032278.01.T01:CDS |
| AACCATTTGCAACCAACTAT+TGG | 0.295534 | 6:+37744825 | None:intergenic |
| AACTGCAAGCATTAATATTG+AGG | 0.529202 | 6:-37743361 | MsG0680032278.01.T01:CDS |
| AAGATTATCTTCATAACTTG+AGG | 0.551107 | 6:+37742638 | None:intergenic |
| AAGGTCAAGCTCAACAAGAT+TGG | 0.422289 | 6:+37744627 | None:intergenic |
| AATAGCAAATTTGATTGAAT+TGG | 0.296081 | 6:-37742877 | MsG0680032278.01.T01:CDS |
| AATAGTTGGTTGCAAATGGT+TGG | 0.539432 | 6:-37744823 | MsG0680032278.01.T01:CDS |
| AATGCATTCTTGACATGGAA+AGG | 0.590497 | 6:-37742990 | MsG0680032278.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTAAGTCTAAGAAGTTG+TGG | - | Chr6:37743181-37743200 | MsG0680032278.01.T01:CDS | 30.0% | |
| AAAGTTGAATGCTTTGTAGA+TGG | + | Chr6:37744812-37744831 | None:intergenic | 30.0% | |
| AACTGCAAGCATTAATATTG+AGG | - | Chr6:37744601-37744620 | MsG0680032278.01.T01:CDS | 30.0% | |
| AATGGTATAGATGCAATGTT+AGG | - | Chr6:37744441-37744460 | MsG0680032278.01.T01:CDS | 30.0% | |
| AATTGAATGCATTCTTGACA+TGG | - | Chr6:37744967-37744986 | MsG0680032278.01.T01:CDS | 30.0% | |
| ACATTGACATACCAACAAAA+TGG | + | Chr6:37744005-37744024 | None:intergenic | 30.0% | |
| ATCATCTTTGATATTCGAGT+GGG | - | Chr6:37743297-37743316 | MsG0680032278.01.T01:CDS | 30.0% | |
| ATTGCATCAAACTCTTTAGA+AGG | - | Chr6:37743733-37743752 | MsG0680032278.01.T01:CDS | 30.0% | |
| CACACCATCTAAATAATTGT+TGG | + | Chr6:37742723-37742742 | None:intergenic | 30.0% | |
| CTAAGCATGAATCAAATCAA+TGG | - | Chr6:37743895-37743914 | MsG0680032278.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 37742377 | 37745607 | 37742377 | ID=MsG0680032278.01;Name=MsG0680032278.01 |
| Chr6 | mRNA | 37742377 | 37745607 | 37742377 | ID=MsG0680032278.01.T01;Parent=MsG0680032278.01;Name=MsG0680032278.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|1076 |
| Chr6 | exon | 37742377 | 37745607 | 37742377 | ID=MsG0680032278.01.T01:exon:20557;Parent=MsG0680032278.01.T01 |
| Chr6 | CDS | 37742377 | 37745607 | 37742377 | ID=MsG0680032278.01.T01:cds;Parent=MsG0680032278.01.T01 |
| Gene Sequence |
>MsG0680032278.01.T01 ATGTTGTCATCTTGGACAACGGAAGATTGTTGCCAATGGTATGCAATTGGTTGCAGTAACATCACCGGTCATGTACTTATGCTCGACCTTCATGGAGATTATAATAATTACTATATCAGAGGTGATTATAATTACTATATCAAAGGTGATATCCACAAGTCACTGATGGAGTTGCAGCAATTAAAGTATTTGAACCTCGGTGGAAATGATTTTCACACTTATATCCCAGGTTTCTTTGGATCTCTAAGAAACCTAAGATACCTTGATCTTTCATATTCTAATTTTTACGGACAAATTCCTGTTCAATTTGAGTATCTTTCACATTTGAAATACTTGAATCTTTCCAACAATTATTTAGATGGTGTGATTCCTCATCGACTTGGAGATCTCTCCAACTTGCAATTTCTTGATCTCAGCAACAATGACTTGGAAGGAAGCATTCCATGTCAACTTGGAAATCTTTCCAACTTGCAATTTCTTGATCTCAGCTGGAATGGTTTGGAAGGAAGCATTCCATCTCAACTTGGATATCTCTCCAACTTGCAGTATTTGGATCTCCTTGGAAATTCTTTAGAAGGAAGTATTCCATCTCAACTTGGAAAGCTTACGAGCCTACAAGAGCTTTATCTTGAAGGAGGAGGCCGATACCGTGATAGTGCTCTAACAATTGACAACGAGGATCACAATGGAGGTCAGTGGTTGTCCAATCTAACTTCTTTAACCCATCTTCACATGTTGTCGATATCTAATCTTGATAAATCCAATAGTTGGTTGCAAATGGTTGGTAAGATACCAAAATTGAGAAAACTAAGTCTAAGAAGTTGTGGCCTTTCTGATCATTTTATCCATTCCTTGAGTCAATCCAAGTTCAACTTTTCCACTTCTCTTTCTGTCCTTCATCTCTCTGCGAACAACTTTGCATCATCTTTGATATTCGAGTGGGTGTCAAACATAAGTTCCAATCTTGTTGAGCTTGACCTTTCTTACAACCTCTTGGTGGCTCCTCCATCACATGGTTATGGCACAGTGATGAAGTCCCTTCAGGTACTTGACCTTTCAAATAATAAATTGAACAGTGGGGCATATAAATCCTTCATGAATTTATGTGCCTTACGTTCTTTAAAACTGTTTCTAAACAATATCACAGAAGACCTTCAAACAATTCTTCAAAATTTGTCTAGTGGTTGTGTTAGAAACTCACTTCAAGTGTTAGACCTCAGTTATAATGGAATCACTGGAACACTACCCGACCTTTCTGCATTCACATCATTGAAAACATTGTCTCTTAATGATAATCAATTGAGTGGAAAGATTCCTGAAGGTAGTAGCTTGCCATTTCAGTTGGAGAGTTTATCCATTGCATCAAACTCTTTAGAAGGGGGGATTCCAAAAACATTTTGGATGAATGCATGTAAGTTGAAAGCATTAGACCTATCCAATAACAGTTTCAGTGGTGAGCTTCAAGTATTAATCCATCACTTATCTAGATGTGCTAGATATTCACTCCAAGAATTAGATCTAAGCATGAATCAAATCAATGGCACACTATCCAATCTCTCAACATTCTCATCTTTGAAAATATTTGATATTTCTGAAAATAAAGTAAATGGTGTGATCTCAGACTTCCATTTTGTTGGTATGTCAATGTTAAAGGGATTATCCTTGTCCGACAATTCACTAGCATTGACATTTACTGAAAATTGGTTCCCACCTTTTCAACTGCACGATATAAGATTGAGATCATGCAAACTTGGTCTTACATTTCCTAAATGGATTCAGACACAAAAACACATGCTGGATCTTGATATTTCGAATGCTGGAATTTCAGATAATGTTCCAGAGTGGTTTTGGCCTAAACTATCATCACAAGAGTGCATAAGCATCAACATTTCAAACAATCATCTCAAAGGATCAATTCCAAATCTTCAAGTCAAAAATCATTGTTTACTTTTATCTCTTTCATCAAATGAATTTGAAGGGCCTATTCCACCATTTCTAATAGGTTCTGCACTTATTGATTTGTCCCAAAATAAATTCTCCGATTCTCTTCCACTCTTATGTGCAAATGGTATAGATGCAATGTTAGGCACGTTAGACCTTTCAGATAATCAATTATCTGGACGAATTCCAGACTGTTGGAGCAATTTCATGTCATTAGTTTGTGTAGATTTGAGCCACAACAATTTTTCAGGGAAGATTCCTACTTCAATGGGATCACTTGTAGAACTGCAAGCATTAATATTGAGGAACAACAGCTTAACAGAGAAGATCCCTTCCTCCTTAAAGAACTGCACAAAGTTAGTAATGCTTGATTTGAGAGAAAATAGATTAGAAGGACTTATCCCTTATTGGATTGGAAGCGAATTAAAAGAGTTGCAGATTTTAAGCTTACAAAGGAATCAATTCTTTGGAATTTTACCATTGGAACTCTGCCATCTACAAAGCATTCAACTTTTTGATCTCTCATTGAACAATCTATCTGGACATATTCCAAAATGCATACAGAATTTAACTTCAATGACTCAAAAAGCTTCATCACAAGGTTTTTCCAATCATGCATATATTATCAACAACCGAGGTGTTCGATATGAATTGAATGCATTCTTGACATGGAAAGGTGTAGAACATGTGTTCAAAAACAATGAATTGTTTCTTCTAAAAAGCATAGATCTCTCAAGCAATCACTTTTCCGGAGAGATTCCACCTGAAATAGCAAATTTGATTGAATTGGTCTCATTGAACTTATCAAGAAACAACTTGACAGGGAAAATTCCTTCAAATATCGGAAAGTTAACATCACTTGACTTTCTTGATTTGTCACAAAACAAGTTGCTTGGTTCAATTCCTTCTAGTCTTTCTCAAATTGATCGACTTAGTGTGTTAGATTTGTCACATAATCAATTATATGGAGAGATTCCAACGAGTACACAGCTACAAAGTTTCAATGCCTCAAGTTATGAAGATAATCTTGATCTTTGTGGAGCACCACTTGTAAAATTGTGCGTTGAAGGGGAGCCACCACTTGAACCAAAAGCAGAAGTTCAAGAAGATGAAGACTTGATTCTAAATCGTGGATTTTACATAAGTCTGACATTCGGATTTATTATTGGCTTTTGGGGAGTATTTGGCTCAATCCTAATCAAGCGTTCTTGGAGACATGCTTATTTCAAGTTCATGAACAACTTAGTGGATACTATTTACGTGAAATGCAGATGGGGGCTCAAAGGTTAA |
| Protein sequence |
>MsG0680032278.01.T01 MLSSWTTEDCCQWYAIGCSNITGHVLMLDLHGDYNNYYIRGDYNYYIKGDIHKSLMELQQLKYLNLGGNDFHTYIPGFFGSLRNLRYLDLSYSNFYGQIPVQFEYLSHLKYLNLSNNYLDGVIPHRLGDLSNLQFLDLSNNDLEGSIPCQLGNLSNLQFLDLSWNGLEGSIPSQLGYLSNLQYLDLLGNSLEGSIPSQLGKLTSLQELYLEGGGRYRDSALTIDNEDHNGGQWLSNLTSLTHLHMLSISNLDKSNSWLQMVGKIPKLRKLSLRSCGLSDHFIHSLSQSKFNFSTSLSVLHLSANNFASSLIFEWVSNISSNLVELDLSYNLLVAPPSHGYGTVMKSLQVLDLSNNKLNSGAYKSFMNLCALRSLKLFLNNITEDLQTILQNLSSGCVRNSLQVLDLSYNGITGTLPDLSAFTSLKTLSLNDNQLSGKIPEGSSLPFQLESLSIASNSLEGGIPKTFWMNACKLKALDLSNNSFSGELQVLIHHLSRCARYSLQELDLSMNQINGTLSNLSTFSSLKIFDISENKVNGVISDFHFVGMSMLKGLSLSDNSLALTFTENWFPPFQLHDIRLRSCKLGLTFPKWIQTQKHMLDLDISNAGISDNVPEWFWPKLSSQECISINISNNHLKGSIPNLQVKNHCLLLSLSSNEFEGPIPPFLIGSALIDLSQNKFSDSLPLLCANGIDAMLGTLDLSDNQLSGRIPDCWSNFMSLVCVDLSHNNFSGKIPTSMGSLVELQALILRNNSLTEKIPSSLKNCTKLVMLDLRENRLEGLIPYWIGSELKELQILSLQRNQFFGILPLELCHLQSIQLFDLSLNNLSGHIPKCIQNLTSMTQKASSQGFSNHAYIINNRGVRYELNAFLTWKGVEHVFKNNELFLLKSIDLSSNHFSGEIPPEIANLIELVSLNLSRNNLTGKIPSNIGKLTSLDFLDLSQNKLLGSIPSSLSQIDRLSVLDLSHNQLYGEIPTSTQLQSFNASSYEDNLDLCGAPLVKLCVEGEPPLEPKAEVQEDEDLILNRGFYISLTFGFIIGFWGVFGSILIKRSWRHAYFKFMNNLVDTIYVKCRWGLKG* |
