Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032301.01.T01 | XP_013451971.3 | 79.651 | 1204 | 216 | 5 | 1 | 1197 | 25 | 1206 | 0 | 1854 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032301.01.T01 | A0A396HF36 | 79.651 | 1204 | 216 | 5 | 1 | 1197 | 25 | 1206 | 0.0 | 1854 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032257.01 | MsG0680032301.01 | 0.824881 | 6.348906e-54 | 5.696271e-51 |
| MsG0680032273.01 | MsG0680032301.01 | 0.809189 | 2.139949e-50 | 1.248147e-47 |
| MsG0680032274.01 | MsG0680032301.01 | 0.822607 | 2.163849e-53 | 1.820116e-50 |
| MsG0680032279.01 | MsG0680032301.01 | 0.822045 | 2.922615e-53 | 2.418786e-50 |
| MsG0680032290.01 | MsG0680032301.01 | 0.835978 | 1.224574e-56 | 1.521997e-53 |
| MsG0680032301.01 | MsG0680032310.01 | 0.805455 | 1.323800e-49 | 7.006835e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032301.01.T01 | MTR_6g039180 | 79.568 | 1204 | 217 | 5 | 1 | 1197 | 4 | 1185 | 0.0 | 1849 |
| MsG0680032301.01.T01 | MTR_6g037750 | 76.591 | 1226 | 238 | 5 | 1 | 1198 | 1 | 1205 | 0.0 | 1808 |
| MsG0680032301.01.T01 | MTR_6g038730 | 75.145 | 1203 | 244 | 6 | 1 | 1198 | 1 | 1153 | 0.0 | 1719 |
| MsG0680032301.01.T01 | MTR_6g038700 | 71.394 | 1255 | 280 | 5 | 1 | 1198 | 4 | 1236 | 0.0 | 1698 |
| MsG0680032301.01.T01 | MTR_6g038940 | 71.580 | 1228 | 208 | 9 | 1 | 1198 | 4 | 1120 | 0.0 | 1626 |
| MsG0680032301.01.T01 | MTR_6g038670 | 69.794 | 1215 | 255 | 10 | 1 | 1197 | 1 | 1121 | 0.0 | 1587 |
| MsG0680032301.01.T01 | MTR_6g038910 | 76.048 | 1073 | 204 | 5 | 127 | 1193 | 45 | 1070 | 0.0 | 1556 |
| MsG0680032301.01.T01 | MTR_6g039110 | 70.685 | 1037 | 246 | 9 | 166 | 1197 | 104 | 1087 | 0.0 | 1344 |
| MsG0680032301.01.T01 | MTR_6g039110 | 37.559 | 1057 | 429 | 25 | 4 | 901 | 1 | 985 | 7.41e-155 | 492 |
| MsG0680032301.01.T01 | MTR_6g038790 | 81.933 | 631 | 109 | 1 | 563 | 1193 | 1 | 626 | 0.0 | 1009 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032301.01.T01 | AT2G34930 | 32.892 | 830 | 441 | 21 | 369 | 1126 | 117 | 902 | 5.37e-97 | 331 |
| MsG0680032301.01.T01 | AT2G34930 | 29.244 | 913 | 507 | 28 | 34 | 877 | 32 | 874 | 1.15e-58 | 219 |
| MsG0680032301.01.T01 | AT4G04220 | 32.542 | 716 | 416 | 19 | 437 | 1133 | 102 | 769 | 2.95e-77 | 273 |
| MsG0680032301.01.T01 | AT4G04220 | 27.880 | 764 | 444 | 27 | 35 | 727 | 24 | 751 | 4.20e-33 | 139 |
| MsG0680032301.01.T01 | AT4G04220 | 28.971 | 680 | 393 | 31 | 210 | 855 | 110 | 733 | 4.03e-28 | 123 |
| MsG0680032301.01.T01 | AT4G04220 | 31.489 | 235 | 134 | 3 | 865 | 1098 | 86 | 294 | 9.73e-17 | 86.3 |
| MsG0680032301.01.T01 | AT1G74190 | 29.797 | 839 | 431 | 21 | 496 | 1188 | 134 | 960 | 3.64e-76 | 273 |
| MsG0680032301.01.T01 | AT1G74190 | 27.184 | 927 | 458 | 34 | 35 | 829 | 27 | 868 | 2.11e-43 | 172 |
| MsG0680032301.01.T01 | AT1G74170 | 30.075 | 931 | 536 | 29 | 327 | 1193 | 82 | 961 | 3.13e-75 | 270 |
| MsG0680032301.01.T01 | AT1G74170 | 28.202 | 773 | 438 | 30 | 126 | 877 | 192 | 868 | 2.93e-36 | 149 |
Find 219 sgRNAs with CRISPR-Local
Find 255 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTAAGGACACTACAGAT+GGG | 0.662728 | 6:-38030259 | MsG0680032301.01.T01:CDS |
| AAACTTAGTTCTCTCAGTTT+TGG | 0.323889 | 6:+38031261 | None:intergenic |
| AAATGACTGATAAAACCATC+TGG | 0.501634 | 6:+38029293 | None:intergenic |
| AACCACAAAATGAAAGATCA+AGG | 0.513126 | 6:+38031703 | None:intergenic |
| AACCTACAAGAGCTTTATCT+TGG | 0.385868 | 6:-38031426 | MsG0680032301.01.T01:CDS |
| AACGGCAATCTACTACCTTC+AGG | 0.449712 | 6:+38030586 | None:intergenic |
| AACTAATGTTTGACACCCAC+TGG | 0.471814 | 6:+38031118 | None:intergenic |
| AAGATAATCTTGATCTTTGT+GGG | 0.397569 | 6:-38028857 | MsG0680032301.01.T01:CDS |
| AAGATTATCTTCATAAGATG+AGG | 0.592979 | 6:+38028869 | None:intergenic |
| AAGGTCAAGCTCAACAAGAT+TGG | 0.436963 | 6:+38031095 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATGACTGATAAAACCATC+TGG | + | Chr6:38031506-38031525 | None:intergenic | 30.0% | |
| AACCACAAAATGAAAGATCA+AGG | + | Chr6:38029096-38029115 | None:intergenic | 30.0% | |
| AATCTTGATATTTCGAATGC+TGG | - | Chr6:38030765-38030784 | MsG0680032301.01.T01:CDS | 30.0% | |
| AATGGTATAGATGCAATGTT+AGG | - | Chr6:38031032-38031051 | MsG0680032301.01.T01:CDS | 30.0% | |
| AATTCACTTCCAATCCAATA+AGG | + | Chr6:38031314-38031333 | None:intergenic | 30.0% | |
| ATATTGACAACATGTGAAGA+TGG | + | Chr6:38029468-38029487 | None:intergenic | 30.0% | |
| ATATTTACGTGAAATGCAGA+TGG | - | Chr6:38032170-38032189 | MsG0680032301.01.T01:CDS | 30.0% | |
| ATCAGATCAAACTCTTTAGA+AGG | - | Chr6:38030252-38030271 | MsG0680032301.01.T01:CDS | 30.0% | |
| ATCTGGTATGTCAATGTTAA+AGG | - | Chr6:38030599-38030618 | MsG0680032301.01.T01:CDS | 30.0% | |
| ATTCACTTCCAATCCAATAA+GGG | + | Chr6:38031313-38031332 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 38028611 | 38032207 | 38028611 | ID=MsG0680032301.01;Name=MsG0680032301.01 |
| Chr6 | mRNA | 38028611 | 38032207 | 38028611 | ID=MsG0680032301.01.T01;Parent=MsG0680032301.01;Name=MsG0680032301.01.T01;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|1198 |
| Chr6 | exon | 38028611 | 38032207 | 38028611 | ID=MsG0680032301.01.T01:exon:20616;Parent=MsG0680032301.01.T01 |
| Chr6 | CDS | 38028611 | 38032207 | 38028611 | ID=MsG0680032301.01.T01:cds;Parent=MsG0680032301.01.T01 |
| Gene Sequence |
>MsG0680032301.01.T01 ATGTCAATGATGAATCCATTTTGTTTCAAATTGATTCAAACAATATTTCTCATATTTTTGGTGTTGCAATCAGACCTTGTTTGTTCAAACGATGATGTTAGGTGCATACAAAATGAGAGGCAAGCACTCCTACAGTTCAAGGCTGGCGTTATTGATGAGCATGGCATGTTGTCATCTTGGACGACCGAAGATTGCTGCCAATGGTATGGAATTGGCTGCAGCAACCTCACTGGCCATGTACTTATGCTCGACCTTCATGGTAATTATAGTCATCATAATCAGCAGTTAAGGATGGTTAAGGATGATTTTGATTACTATTTAGGTTACTATGGTTTTATGGATGTCAATACATTTTATATCAGCGGTGACATCCACAAGTCACTGATGGAGTTGCAACATTTACAATATTTGAACCTTAGCAGAAATAATTTTGAAGAAAGTTATATCCCAGGTTTCATTGGATCTCTAAGAAACCTAAGATACCTTGATCTTTCATTTTGTGGTTTTGGAGGACAAATTCCTATTCAACTTGAGTCTCTTCCACATTTGAAATACTTGAATCTTTCCAACAATCATCTAGATGGTGTGATTCCTCATCGACTTGGAGATCTCTCCAACTTGCAATTTCTTGATCTCAGCAACAATCATTTGGAAGGAAGCATTCCATCTCAACTTGGAAATCTCTCCAACTTGCAATTTCTTGGTCTCCATGGATATTATTTAAAAGGAAAAATACCATCTCAGCTTGGAAAGCTTACGAACCTACAAGAGCTTTATCTTGGAGAAGGAGATCTAACAATTGACAACGAGGATCACAACGGAGGTCAGTGGTTGTCTAATCTTACTTCTTTAACCCATCTTCACATGTTGTCAATATCTAATCTTGATAAATTTAATAGTTGGTTGCAAATGGTTGGCAAGTTACCAAAACTGAGAGAACTAAGTTTACAAAGTTGTGGCCTTTCTGATCATTTTATCCATTCCTTGAGTCATTCCAAGTTCAAATTTTCCAATTCTCTTTCCATCCTCGATCTCTCTGGGAACAACTTAGCGTCATCTTTGATATTCCAGTGGGTGTCAAACATTAGTTCCAATCTTGTTGAGCTTGACCTTAGTTCGAACCAGATAGTGGATCCCCTGTCAAATCATTTTTCCTGCAGACTTCCAAAATTGAAAAAACTGAGATTATCTGAAAACAAGTTCACATCATTCATGATATTTCAATGGGTGTCAAACATAAGTTCCAATCTCGTTGAACTTGACCTACGTGGTAATGAAATGGTGGATCCACCATCAAATGGCTATGGCATAGAGATGAAGTCTCTTGAGGTACTTGACTTGTCATTCAATAGATTGAAGGGTGGGGCGTTTAAATCTTTCATGAATGTATGCACCTTACGTTCTTTATATCTGAATGATAACTACTTAATTGAAGACCTTCAATCAATTATTCACAATTTGTCTAGTGGTTGTGTTAGAAACTCACTTCAAGTGTTAGACCTCAGTGTTAATGTAATCACTGGAACACTACCCGACCTTTCTATATTCACATCATTGAAAACATTGGACCTTTCTGATAATAAGCTGAGTGGAAAGATTCCTGAAGGTAGTAGATTGCCGTTTCAGTTGGAGAGTTTATCCATCAGATCAAACTCTTTAGAAGGGGGGATTCCAAAATCATTTTGGCTGAATGCATGTAAGTTGAAATCATTGGACCTGTCCAATAACAGTTTCAGTGGTGAGCTTCAAGTAATCATCCATCACTTATCTAGATGTGCTAGAAAATCACTTCAACAATTAGATCTAAGAAGTAATCAAATCAATGGCACACTACCTGATCTCTCAATATTTTCATTTTTAGAAACATTTCATATTTCTGAAAATAGCCTAAACGGGAAGGTAGCTGAAGATATTCGATTTCCAACCAAACTAAGGACACTACAGATGGGCTTGAACTATATGAGTGGTGTGATCTCTGACTTCCAGTTATCTGGTATGTCAATGTTAAAGGATTTAGACTTGTCAGACAATTCACTAGCATTGAGATTTACCGAAAACTGGGTCCCGCCTTTTCAACTGTATAATATAAGATTGAGATCATGCAAACTTGGTCCTACATTTCCTAAATGGATTCAGACACAAAAATACCTGCAGAATCTTGATATTTCGAATGCTGGAATTTCAGATAATGTTCCAGTGTGGTTTTGGGCTAAACTATCATCACAAGAGTGCAGAAGCATAAACATTTCAAACAATCATCTCAAAGGATCAATTTCAAATCTTCAAGTCAAAAATCGTTGTTCTTTTTTAGATTTTTCATCAAATGAATTTCAAGGGCCTATTCCACCATTTCTACGAGGTTCTGCACTTATTGATCTGTCCAAAAATAAATTCACTGATTCTCTTCCATTCCTATGTGCAAATGGTATAGATGCAATGTTAGGCCAGTTTGACCTTTCGAATAATCAATTATCTGGACGAATTCCAGACTGTTGGAGCAAGTTCAAACCATTAGCTTATGTAGATTTGAGCCACAACAATTTTTCAGGGAAGATTCCTTCTTCAATGGGATCACTTGTAGAATTGGAAGCATTACTATTGAGGAACAACAGCTTAACAGAGGAGATCCCTTCCTCCTTAATGAACTGCACAAAGTTAGTAATGCTTGATTTGAGAGAAAATAGATTAAATGGACTCATCCCTTATTGGATTGGAAGTGAATTAAAAGATCTGCAAATTTTAAGCTTACAAAGGAATCATTTCTTTGGAAGTTTACCATTTGAACTTTGCCATCTACAAAATATTCAACTTTTTGATCTCTCACTGAACAATCTCTCTGGGCAGATTCCAAAATGCTTTAAAAATTTCACTTCAATGACTCAAAAAGATTCACCAGATGGTTTTATCAGTCATTTTTATTATATTAACCAAGGATCTGCGAGTTTTCAAGAAGTCTATGAATTGAGTGCATTCTTGACATGGAAAGGTGTAGAACAACTGTTCGCCAACAATGAATTGTTTCTTTTAAAAAGCATAGATCTCTCAAGCAATCATTTTTCAGGAGAGATTCCACCTGAAATAGCTAATTTGATTCAATTGGTCTCATTGAACTTATCAAGAAACAACTTCAAAGGGAAAATTCCTTCAAGAATTGGAAAGCTAACATCACTTGACTTTCTTGATTTGTCGCGAAATAAGTTGCTTGGTTCAATTCCGTCCAGTCTTTCCCGAATAGATCGACTTTCTGTGTTAGATTTATCACATAATCAATTATCTGGAGAGATTCCAACAAGTACACAACTACAAAGTTTCAATGCCTCATCTTATGAAGATAATCTTGATCTTTGTGGGCCGCCACTTGCAAAACTGTGTGTCCAACGGAAGCCACAACATGAAGCAAAAGTAAAAATTCAAGATGAAGATTTGCTTCTCCATCGTGGATTTTACATCAGTCTGACATTCGGATTTATTATAGGATTTTGGGGTGTGTTTGGCTCAATCTTAATCAAACGTTCTTGGAGACATGCTTATTTCAAGTTCATGAACAACTTAGTGGATAATATTTACGTGAAATGCAGATGGGGGCTCAAAGATTAA |
| Protein sequence |
>MsG0680032301.01.T01 MSMMNPFCFKLIQTIFLIFLVLQSDLVCSNDDVRCIQNERQALLQFKAGVIDEHGMLSSWTTEDCCQWYGIGCSNLTGHVLMLDLHGNYSHHNQQLRMVKDDFDYYLGYYGFMDVNTFYISGDIHKSLMELQHLQYLNLSRNNFEESYIPGFIGSLRNLRYLDLSFCGFGGQIPIQLESLPHLKYLNLSNNHLDGVIPHRLGDLSNLQFLDLSNNHLEGSIPSQLGNLSNLQFLGLHGYYLKGKIPSQLGKLTNLQELYLGEGDLTIDNEDHNGGQWLSNLTSLTHLHMLSISNLDKFNSWLQMVGKLPKLRELSLQSCGLSDHFIHSLSHSKFKFSNSLSILDLSGNNLASSLIFQWVSNISSNLVELDLSSNQIVDPLSNHFSCRLPKLKKLRLSENKFTSFMIFQWVSNISSNLVELDLRGNEMVDPPSNGYGIEMKSLEVLDLSFNRLKGGAFKSFMNVCTLRSLYLNDNYLIEDLQSIIHNLSSGCVRNSLQVLDLSVNVITGTLPDLSIFTSLKTLDLSDNKLSGKIPEGSRLPFQLESLSIRSNSLEGGIPKSFWLNACKLKSLDLSNNSFSGELQVIIHHLSRCARKSLQQLDLRSNQINGTLPDLSIFSFLETFHISENSLNGKVAEDIRFPTKLRTLQMGLNYMSGVISDFQLSGMSMLKDLDLSDNSLALRFTENWVPPFQLYNIRLRSCKLGPTFPKWIQTQKYLQNLDISNAGISDNVPVWFWAKLSSQECRSINISNNHLKGSISNLQVKNRCSFLDFSSNEFQGPIPPFLRGSALIDLSKNKFTDSLPFLCANGIDAMLGQFDLSNNQLSGRIPDCWSKFKPLAYVDLSHNNFSGKIPSSMGSLVELEALLLRNNSLTEEIPSSLMNCTKLVMLDLRENRLNGLIPYWIGSELKDLQILSLQRNHFFGSLPFELCHLQNIQLFDLSLNNLSGQIPKCFKNFTSMTQKDSPDGFISHFYYINQGSASFQEVYELSAFLTWKGVEQLFANNELFLLKSIDLSSNHFSGEIPPEIANLIQLVSLNLSRNNFKGKIPSRIGKLTSLDFLDLSRNKLLGSIPSSLSRIDRLSVLDLSHNQLSGEIPTSTQLQSFNASSYEDNLDLCGPPLAKLCVQRKPQHEAKVKIQDEDLLLHRGFYISLTFGFIIGFWGVFGSILIKRSWRHAYFKFMNNLVDNIYVKCRWGLKD* |
