Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032479.01.T01 | XP_013441370.1 | 93.407 | 91 | 6 | 0 | 1 | 91 | 20 | 110 | 2.71E-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032479.01.T01 | A0A072TPC9 | 93.407 | 91 | 6 | 0 | 1 | 91 | 20 | 110 | 1.29e-55 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032479.01 | MsG0880041864.01 | 0.806159 | 9.419383e-50 | 5.076447e-47 |
| MsG0480023489.01 | MsG0680032479.01 | 0.811152 | 8.075389e-51 | 4.961161e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032479.01.T01 | MTR_1173s0010 | 93.407 | 91 | 6 | 0 | 1 | 91 | 20 | 110 | 3.28e-59 | 176 |
| MsG0680032479.01.T01 | MTR_3g434890 | 92.308 | 91 | 7 | 0 | 1 | 91 | 20 | 110 | 1.98e-58 | 174 |
| MsG0680032479.01.T01 | MTR_3g434960 | 91.209 | 91 | 8 | 0 | 1 | 91 | 20 | 110 | 1.23e-57 | 172 |
| MsG0680032479.01.T01 | MTR_7g451200 | 91.209 | 91 | 8 | 0 | 1 | 91 | 20 | 110 | 2.08e-57 | 172 |
| MsG0680032479.01.T01 | MTR_7g451210 | 88.889 | 90 | 10 | 0 | 1 | 90 | 20 | 109 | 1.78e-55 | 167 |
| MsG0680032479.01.T01 | MTR_3g434920 | 90.361 | 83 | 8 | 0 | 9 | 91 | 2 | 84 | 1.10e-51 | 157 |
| MsG0680032479.01.T01 | MTR_3g435000 | 84.706 | 85 | 13 | 0 | 1 | 85 | 24 | 108 | 6.11e-49 | 150 |
| MsG0680032479.01.T01 | MTR_6g049260 | 68.132 | 91 | 29 | 0 | 1 | 91 | 20 | 110 | 1.45e-40 | 129 |
| MsG0680032479.01.T01 | MTR_6g049320 | 57.955 | 88 | 35 | 1 | 1 | 86 | 20 | 107 | 1.39e-23 | 87.0 |
| MsG0680032479.01.T01 | MTR_6g049280 | 56.818 | 88 | 36 | 1 | 1 | 86 | 20 | 107 | 2.06e-23 | 86.7 |
| MsG0680032479.01.T01 | MTR_6g049320 | 57.955 | 88 | 35 | 1 | 1 | 86 | 50 | 137 | 2.30e-23 | 87.4 |
| MsG0680032479.01.T01 | MTR_6g049280 | 56.818 | 88 | 36 | 1 | 1 | 86 | 50 | 137 | 5.42e-23 | 86.7 |
| MsG0680032479.01.T01 | MTR_1g099800 | 66.000 | 50 | 17 | 0 | 1 | 50 | 20 | 69 | 3.00e-18 | 73.9 |
| MsG0680032479.01.T01 | MTR_1g099810 | 66.000 | 50 | 17 | 0 | 1 | 50 | 1 | 50 | 1.74e-17 | 71.6 |
| MsG0680032479.01.T01 | MTR_1g099810 | 66.000 | 50 | 17 | 0 | 1 | 50 | 20 | 69 | 2.46e-17 | 71.6 |
| MsG0680032479.01.T01 | MTR_6g051680 | 60.000 | 50 | 20 | 0 | 1 | 50 | 20 | 69 | 3.90e-16 | 67.8 |
| MsG0680032479.01.T01 | MTR_8g098915 | 56.604 | 53 | 23 | 0 | 1 | 53 | 1 | 53 | 8.69e-16 | 67.0 |
| MsG0680032479.01.T01 | MTR_8g098915 | 56.604 | 53 | 23 | 0 | 1 | 53 | 20 | 72 | 1.64e-15 | 66.6 |
| MsG0680032479.01.T01 | MTR_6g051690 | 60.000 | 50 | 20 | 0 | 1 | 50 | 20 | 69 | 1.71e-15 | 66.6 |
| MsG0680032479.01.T01 | MTR_1g099640 | 44.330 | 97 | 42 | 2 | 1 | 85 | 20 | 116 | 2.89e-15 | 66.2 |
| MsG0680032479.01.T01 | MTR_1g099815 | 62.000 | 50 | 18 | 1 | 1 | 50 | 20 | 68 | 5.39e-14 | 62.8 |
| MsG0680032479.01.T01 | MTR_1g102240 | 62.000 | 50 | 18 | 1 | 1 | 50 | 20 | 68 | 5.45e-14 | 62.8 |
| MsG0680032479.01.T01 | MTR_8g028615 | 53.191 | 47 | 22 | 0 | 3 | 49 | 22 | 68 | 2.98e-12 | 58.9 |
| MsG0680032479.01.T01 | MTR_7g079110 | 53.061 | 49 | 23 | 0 | 1 | 49 | 20 | 68 | 3.00e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032479.01.T01 | AT1G01490 | 54.902 | 51 | 22 | 1 | 1 | 50 | 47 | 97 | 1.57e-11 | 57.8 |
| MsG0680032479.01.T01 | AT1G01490 | 54.902 | 51 | 22 | 1 | 1 | 50 | 20 | 70 | 2.05e-11 | 57.4 |
| MsG0680032479.01.T01 | AT1G01490 | 54.902 | 51 | 22 | 1 | 1 | 50 | 20 | 70 | 2.05e-11 | 57.4 |
Find 12 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGCACACTGCCTTTAC+TGG | 0.181442 | 6:-42427448 | None:intergenic |
| GAGAAGAAATTGACCTTATC+AGG | 0.225200 | 6:+42427415 | MsG0680032479.01.T01:CDS |
| AAGACAGCATCTAGCCTTCC+AGG | 0.337922 | 6:+42426809 | MsG0680032479.01.T01:CDS |
| GGGGTCATCTGATAATAAAA+TGG | 0.395316 | 6:-42427580 | None:intergenic |
| TGGATCAATATCCCCTGATA+AGG | 0.488192 | 6:-42427428 | None:intergenic |
| ACCTCTAAAAGATGAGAAGA+AGG | 0.498625 | 6:+42427507 | MsG0680032479.01.T01:CDS |
| TAGGTTTCAAAGTATTGAAG+TGG | 0.516403 | 6:-42427556 | None:intergenic |
| AGAAGAAATTGACCTTATCA+GGG | 0.561907 | 6:+42427416 | MsG0680032479.01.T01:CDS |
| AGGGGATATTGATCCAGTAA+AGG | 0.575600 | 6:+42427435 | MsG0680032479.01.T01:CDS |
| CATCTGATAATAAAATGGAT+AGG | 0.609597 | 6:-42427575 | None:intergenic |
| ATTCAATATATATACCTGGA+AGG | 0.616834 | 6:-42426823 | None:intergenic |
| GAAGAAATTGACCTTATCAG+GGG | 0.672768 | 6:+42427417 | MsG0680032479.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATTACATTAATTTGATA+AGG | + | Chr6:42427150-42427169 | MsG0680032479.01.T01:intron | 10.0% |
| !! | AATATTAAAATCAATCATGA+TGG | - | Chr6:42427111-42427130 | None:intergenic | 15.0% |
| !! | ATAAAGTTATATGTTAGAAA+TGG | - | Chr6:42427177-42427196 | None:intergenic | 15.0% |
| !!! | TAGTAATTTTGAAAATATTG+TGG | - | Chr6:42427607-42427626 | None:intergenic | 15.0% |
| !! | ACTAAACATATCAAATGTTA+CGG | + | Chr6:42427057-42427076 | MsG0680032479.01.T01:intron | 20.0% |
| !!! | AATTTTGAAAATATTGTGGT+GGG | - | Chr6:42427603-42427622 | None:intergenic | 20.0% |
| !!! | TAACTAATGCTGAAATTTTT+TGG | - | Chr6:42426924-42426943 | None:intergenic | 20.0% |
| !!! | TAATTTTGAAAATATTGTGG+TGG | - | Chr6:42427604-42427623 | None:intergenic | 20.0% |
| !!! | TTTTTTCTTTCTATTTTGCA+GGG | + | Chr6:42427365-42427384 | MsG0680032479.01.T01:intron | 20.0% |
| !!! | TTTTTTTCTTTCTATTTTGC+AGG | + | Chr6:42427364-42427383 | MsG0680032479.01.T01:intron | 20.0% |
| ! | AATGAATCATTTGTGTTAGA+TGG | - | Chr6:42427345-42427364 | None:intergenic | 25.0% |
| ! | ATTCAATATATATACCTGGA+AGG | - | Chr6:42426826-42426845 | None:intergenic | 25.0% |
| ! | CATCTGATAATAAAATGGAT+AGG | - | Chr6:42427578-42427597 | None:intergenic | 25.0% |
| ! | CATGACTAATAAGTTATAGA+TGG | + | Chr6:42426892-42426911 | MsG0680032479.01.T01:intron | 25.0% |
| ! | TGCAATTCAATATATATACC+TGG | - | Chr6:42426830-42426849 | None:intergenic | 25.0% |
| ! | TTGGCTTACAATTAGAAAAT+CGG | + | Chr6:42426951-42426970 | MsG0680032479.01.T01:intron | 25.0% |
| !! | ATTTTCTAATTGTAAGCCAA+CGG | - | Chr6:42426951-42426970 | None:intergenic | 25.0% |
| !! | TTTTTCTTTCTATTTTGCAG+GGG | + | Chr6:42427366-42427385 | MsG0680032479.01.T01:intron | 25.0% |
| !!! | ATTTTGAAAATATTGTGGTG+GGG | - | Chr6:42427602-42427621 | None:intergenic | 25.0% |
| !!! | CAAACAAAAATAGTATCGAT+TGG | + | Chr6:42427484-42427503 | MsG0680032479.01.T01:CDS | 25.0% |
| !!! | CAATCGATACTATTTTTGTT+TGG | - | Chr6:42427486-42427505 | None:intergenic | 25.0% |
| !!! | TTAACTACCATTAGGTTTTT+TGG | + | Chr6:42426980-42426999 | MsG0680032479.01.T01:intron | 25.0% |
| AGAAGAAATTGACCTTATCA+GGG | + | Chr6:42427416-42427435 | MsG0680032479.01.T01:CDS | 30.0% | |
| GAAATGACCAAAAAACCTAA+TGG | - | Chr6:42426990-42427009 | None:intergenic | 30.0% | |
| !! | TAGGTTTCAAAGTATTGAAG+TGG | - | Chr6:42427559-42427578 | None:intergenic | 30.0% |
| CAGCATTAGTTAATTACCGT+TGG | + | Chr6:42426932-42426951 | MsG0680032479.01.T01:intron | 35.0% | |
| GAAGAAATTGACCTTATCAG+GGG | + | Chr6:42427417-42427436 | MsG0680032479.01.T01:CDS | 35.0% | |
| GAGAAGAAATTGACCTTATC+AGG | + | Chr6:42427415-42427434 | MsG0680032479.01.T01:CDS | 35.0% | |
| GCTTACAATTAGAAAATCGG+CGG | + | Chr6:42426954-42426973 | MsG0680032479.01.T01:intron | 35.0% | |
| GGGGTCATCTGATAATAAAA+TGG | - | Chr6:42427583-42427602 | None:intergenic | 35.0% | |
| ! | ACCTCTAAAAGATGAGAAGA+AGG | + | Chr6:42427507-42427526 | MsG0680032479.01.T01:CDS | 35.0% |
| ! | TCCTTCTTCTCATCTTTTAG+AGG | - | Chr6:42427511-42427530 | None:intergenic | 35.0% |
| !! | CATTGATTCCTAAAATCGCA+TGG | - | Chr6:42427012-42427031 | None:intergenic | 35.0% |
| AGGGGATATTGATCCAGTAA+AGG | + | Chr6:42427435-42427454 | MsG0680032479.01.T01:CDS | 40.0% | |
| AGTACCACTGCTATGTAATG+TGG | + | Chr6:42426861-42426880 | MsG0680032479.01.T01:intron | 40.0% | |
| GGCGGTATTTAACTACCATT+AGG | + | Chr6:42426972-42426991 | MsG0680032479.01.T01:intron | 40.0% | |
| TGGATCAATATCCCCTGATA+AGG | - | Chr6:42427431-42427450 | None:intergenic | 40.0% | |
| TTAGCCACATTACATAGCAG+TGG | - | Chr6:42426868-42426887 | None:intergenic | 40.0% | |
| ! | GGTCATTTCCATGCGATTTT+AGG | + | Chr6:42427001-42427020 | MsG0680032479.01.T01:intron | 40.0% |
| !! | TATTAAAAAAAACTTAAATA+AGG | + | Chr6:42427194-42427213 | MsG0680032479.01.T01:intron | 5.0% |
| AGCTTGCACACTGCCTTTAC+TGG | - | Chr6:42427451-42427470 | None:intergenic | 50.0% | |
| ! | AAGACAGCATCTAGCCTTCC+AGG | + | Chr6:42426809-42426828 | MsG0680032479.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 42426806 | 42427636 | 42426806 | ID=MsG0680032479.01;Name=MsG0680032479.01 |
| Chr6 | mRNA | 42426806 | 42427636 | 42426806 | ID=MsG0680032479.01.T01;Parent=MsG0680032479.01;Name=MsG0680032479.01.T01;_AED=0.43;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|91 |
| Chr6 | exon | 42427386 | 42427636 | 42427386 | ID=MsG0680032479.01.T01:exon:16353;Parent=MsG0680032479.01.T01 |
| Chr6 | exon | 42426806 | 42426830 | 42426806 | ID=MsG0680032479.01.T01:exon:16352;Parent=MsG0680032479.01.T01 |
| Chr6 | CDS | 42426806 | 42426830 | 42426806 | ID=MsG0680032479.01.T01:cds;Parent=MsG0680032479.01.T01 |
| Chr6 | CDS | 42427386 | 42427636 | 42427386 | ID=MsG0680032479.01.T01:cds;Parent=MsG0680032479.01.T01 |
| Gene Sequence |
| Protein sequence |
