AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033509.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033509.01.T01 MTR_6g055360 65.766 222 28 4 66 265 62 257 1.19e-80 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033509.01.T01 AT3G48900 42.986 221 78 5 66 264 62 256 4.42e-44 157
MsG0680033509.01.T01 AT3G48900 41.176 187 64 4 98 264 32 192 3.17e-35 132
MsG0680033509.01.T01 AT3G48900 41.176 187 64 4 98 264 32 192 3.63e-35 132

Find 56 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTAAATTGTTGACTTAT+AGG 0.099496 6:-66870321 MsG0680033509.01.T01:CDS
GGTAATCCTGCAAACAATTT+TGG 0.165840 6:+66870642 None:intergenic
CCTATAAGTCAACAATTTAA+TGG 0.277772 6:+66870321 None:intergenic
TGGCCATTCGAACCAGTTAT+AGG 0.295627 6:+66870662 None:intergenic
ATTGAAAGAAAACTTGGATT+TGG 0.303227 6:-66869411 MsG0680033509.01.T01:CDS
ATTAGGAACCTACTGTAATT+AGG 0.317500 6:+66870591 None:intergenic
GGCCATTCGAACCAGTTATA+GGG 0.333694 6:+66870663 None:intergenic
TCTTGACTAAGACTCCTTCT+AGG 0.346748 6:+66870744 None:intergenic
TGTCAGATAGTCAAATCAAT+TGG 0.360885 6:-66868543 MsG0680033509.01.T01:CDS
GAATGGCCAAAATTGTTTGC+AGG 0.364536 6:-66870648 MsG0680033509.01.T01:CDS
AGAGCCACAGGAGCGTTAAA+TGG 0.366797 6:-66870614 MsG0680033509.01.T01:CDS
ATTTCAGAGGCTAAAATCTT+GGG 0.372759 6:-66870059 MsG0680033509.01.T01:CDS
CTTGGCGTCTCATGTTTAAA+CGG 0.378450 6:-66870029 MsG0680033509.01.T01:CDS
GATTTCAGAGGCTAAAATCT+TGG 0.387928 6:-66870060 MsG0680033509.01.T01:CDS
CAAGAGGTCACAAAATCTAA+TGG 0.389065 6:-66870726 MsG0680033509.01.T01:CDS
CAAGGGATTATCCCTATAAC+TGG 0.402444 6:-66870674 MsG0680033509.01.T01:CDS
GTCAGATAGTCAAATCAATT+GGG 0.404672 6:-66868542 MsG0680033509.01.T01:CDS
AAATTTGCTTCTGAAGGACT+TGG 0.407512 6:-66868501 MsG0680033509.01.T01:CDS
ATTCACACATGTCAAGATCT+TGG 0.414392 6:-66870817 None:intergenic
AATCAAATTCAATTCCTAGA+AGG 0.436915 6:-66870758 MsG0680033509.01.T01:CDS
TGAATTTGATTTATGTTCCT+AGG 0.444165 6:+66870770 None:intergenic
TGGATTTGGACGGGACTCAT+TGG 0.453236 6:-66869397 MsG0680033509.01.T01:intron
AAAATCTTGGGGATGGCTCT+TGG 0.463215 6:-66870047 MsG0680033509.01.T01:CDS
TTGGCGTCTCATGTTTAAAC+GGG 0.464715 6:-66870028 MsG0680033509.01.T01:intron
TTGGTGCAAGGACAGTGTAC+AGG 0.465370 6:-66869593 MsG0680033509.01.T01:CDS
GACATCACTGCGGAGAAACC+TGG 0.466009 6:-66870102 MsG0680033509.01.T01:CDS
GCAGACATTGAAAGAAAACT+TGG 0.486553 6:-66869417 MsG0680033509.01.T01:CDS
TGCTTCTGAAGGACTTGGAT+GGG 0.496736 6:-66868496 MsG0680033509.01.T01:CDS
TAAGTCAACAATTTAATGGC+AGG 0.499585 6:+66870325 None:intergenic
TTGCTTCTGAAGGACTTGGA+TGG 0.517715 6:-66868497 MsG0680033509.01.T01:CDS
AAAGAAAACTTGGATTTGGA+CGG 0.521984 6:-66869407 MsG0680033509.01.T01:CDS
TGCATGAAAATTCAGACCCC+AGG 0.523842 6:+66870084 None:intergenic
ACGCTCCTGTGGCTCTATCA+AGG 0.527425 6:+66870621 None:intergenic
TATAGGCGTCGCTTAAACAA+TGG 0.528136 6:-66870304 MsG0680033509.01.T01:CDS
TTTAATAATGAGTCAGGAGA+CGG 0.541604 6:-66869465 MsG0680033509.01.T01:intron
GGATACGCACTCTGACTCCT+AGG 0.546029 6:-66870787 MsG0680033509.01.T01:CDS
ACATCACTGCGGAGAAACCT+GGG 0.547363 6:-66870101 MsG0680033509.01.T01:CDS
AAGAAAACTTGGATTTGGAC+GGG 0.557062 6:-66869406 MsG0680033509.01.T01:CDS
TGGTGCAAGGACAGTGTACA+GGG 0.558210 6:-66869592 MsG0680033509.01.T01:CDS
ATCCCTATAACTGGTTCGAA+TGG 0.559674 6:-66870665 MsG0680033509.01.T01:CDS
TGTCAAGATCTTGGTTAGAG+TGG 0.561376 6:-66870808 MsG0680033509.01.T01:CDS
AGAGGCTAAAATCTTGGGGA+TGG 0.570300 6:-66870054 MsG0680033509.01.T01:CDS
GATTACCTTGATAGAGCCAC+AGG 0.570847 6:-66870626 MsG0680033509.01.T01:CDS
TCGCTTAAACAATGGAAAAG+AGG 0.580852 6:-66870296 MsG0680033509.01.T01:intron
TTAAATGGCCTAATTACAGT+AGG 0.585411 6:-66870599 MsG0680033509.01.T01:intron
TACGTCTACTCAGTTGAACA+AGG 0.585587 6:-66870692 MsG0680033509.01.T01:CDS
TAGAAGGAGTCTTAGTCAAG+AGG 0.594111 6:-66870742 MsG0680033509.01.T01:CDS
GTGTACAGGGATATCTGCCT+TGG 0.607991 6:-66869579 MsG0680033509.01.T01:intron
TAGGCCATTTAACGCTCCTG+TGG 0.617916 6:+66870610 None:intergenic
TTTCAGAGGCTAAAATCTTG+GGG 0.624768 6:-66870058 MsG0680033509.01.T01:CDS
TCAGATAGTCAAATCAATTG+GGG 0.642856 6:-66868541 MsG0680033509.01.T01:CDS
AATAATGAGTCAGGAGACGG+TGG 0.664112 6:-66869462 MsG0680033509.01.T01:intron
CATCACTGCGGAGAAACCTG+GGG 0.679390 6:-66870100 MsG0680033509.01.T01:CDS
ATATGTTGTATGTTATGAGA+TGG 0.699465 6:-66869439 MsG0680033509.01.T01:CDS
ACGTCTACTCAGTTGAACAA+GGG 0.703699 6:-66870691 MsG0680033509.01.T01:CDS
TACAGAAAACGACATCACTG+CGG 0.760993 6:-66870112 MsG0680033509.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTGATAATTTTCAAAT+GGG - Chr6:66868803-66868822 MsG0680033509.01.T01:intron 10.0%
!! AAAAGAAAAAGAAATAAGAA+AGG + Chr6:66869017-66869036 None:intergenic 15.0%
!! CATTTGAAAATTATCAAATA+AGG + Chr6:66868804-66868823 None:intergenic 15.0%
!! GTTAGATATTACATATTATA+TGG - Chr6:66870674-66870693 MsG0680033509.01.T01:CDS 15.0%
!!! AAAAATGAAAATTTGTTTGT+AGG - Chr6:66869374-66869393 MsG0680033509.01.T01:intron 15.0%
!!! AAAATGAAAATTTGTTTGTA+GGG - Chr6:66869375-66869394 MsG0680033509.01.T01:intron 15.0%
!!! CTTATTTGATAATTTTCAAA+TGG - Chr6:66868802-66868821 MsG0680033509.01.T01:intron 15.0%
!!! TCATTCATTTTGAAAATTAT+AGG - Chr6:66869627-66869646 MsG0680033509.01.T01:CDS 15.0%
!!! TTGTAATTATCAGTTATTTT+TGG - Chr6:66869562-66869581 MsG0680033509.01.T01:intron 15.0%
!! ACCATTTAATATTCAAACAA+TGG + Chr6:66870323-66870342 None:intergenic 20.0%
!! ATAACTGATAATTACAAAGT+AGG + Chr6:66869559-66869578 None:intergenic 20.0%
!! GTAATATTCAATAGAAGTTA+AGG - Chr6:66869273-66869292 MsG0680033509.01.T01:intron 20.0%
!! TAAAAATATCCTATCTTTCA+TGG + Chr6:66870046-66870065 None:intergenic 20.0%
!! TAACTGATAATTACAAAGTA+GGG + Chr6:66869558-66869577 None:intergenic 20.0%
!! TCCATTGTTTGAATATTAAA+TGG - Chr6:66870319-66870338 MsG0680033509.01.T01:CDS 20.0%
!!! AAATGAAAATTTGTTTGTAG+GGG - Chr6:66869376-66869395 MsG0680033509.01.T01:intron 20.0%
!!! AATAATTTTGGTACAATATC+AGG + Chr6:66870418-66870437 None:intergenic 20.0%
!!! GATGAAAATGTCAATAATTT+TGG + Chr6:66870430-66870449 None:intergenic 20.0%
!!! GATTCAGATATATTTCTTTT+TGG - Chr6:66869667-66869686 MsG0680033509.01.T01:CDS 20.0%
!!! GTTTTTTTTAATAATGAGTC+AGG - Chr6:66869808-66869827 MsG0680033509.01.T01:CDS 20.0%
!!! TCATTTTGAAAATTATAGGA+TGG - Chr6:66869631-66869650 MsG0680033509.01.T01:CDS 20.0%
!!! TTTTGATATCAACAATTACA+AGG + Chr6:66869336-66869355 None:intergenic 20.0%
! AACTGATAATTACAAAGTAG+GGG + Chr6:66869557-66869576 None:intergenic 25.0%
! AATCAAATTCAATTCCTAGA+AGG - Chr6:66868521-66868540 MsG0680033509.01.T01:CDS 25.0%
! AGGGTAACTTATATATTCAT+TGG + Chr6:66870273-66870292 None:intergenic 25.0%
! ATATGTTGTATGTTATGAGA+TGG - Chr6:66869840-66869859 MsG0680033509.01.T01:intron 25.0%
! ATTCAATAGAAGTTAAGGTT+TGG - Chr6:66869278-66869297 MsG0680033509.01.T01:intron 25.0%
! ATTGAAAGAAAACTTGGATT+TGG - Chr6:66869868-66869887 MsG0680033509.01.T01:intron 25.0%
! CAAATGAGAAAAACTAATGA+TGG + Chr6:66868850-66868869 None:intergenic 25.0%
! CATTATACATGTCAATTCAA+GGG - Chr6:66870471-66870490 MsG0680033509.01.T01:intron 25.0%
! CCTATAAGTCAACAATTTAA+TGG + Chr6:66868961-66868980 None:intergenic 25.0%
! CTAGAAAAAATTCATATGCA+TGG + Chr6:66870381-66870400 None:intergenic 25.0%
! GTAAAGGTTATACATAGAAT+AGG + Chr6:66869040-66869059 None:intergenic 25.0%
! TATAAGTTACCCTATAAAGA+AGG - Chr6:66870280-66870299 MsG0680033509.01.T01:intron 25.0%
! TCTTTAATGCAAATTCAGTT+GGG - Chr6:66869979-66869998 MsG0680033509.01.T01:intron 25.0%
! TTCTTTAATGCAAATTCAGT+TGG - Chr6:66869978-66869997 MsG0680033509.01.T01:intron 25.0%
!! CAAATTTGTATTTTCAGCAT+AGG + Chr6:66870355-66870374 None:intergenic 25.0%
!! CTAAAAAAATTTGCTTCTGA+AGG - Chr6:66870772-66870791 MsG0680033509.01.T01:CDS 25.0%
!! TGAATTTGATTTATGTTCCT+AGG + Chr6:66868512-66868531 None:intergenic 25.0%
!! TGTATAATGGAACTTTGATT+TGG + Chr6:66870460-66870479 None:intergenic 25.0%
!! TTATGTGCATTTTGTTCATT+AGG + Chr6:66868708-66868727 None:intergenic 25.0%
!!! ATATATTTCTTTTTGGTGCA+AGG - Chr6:66869674-66869693 MsG0680033509.01.T01:CDS 25.0%
!!! CATTTGTTTTTGAATATGCT+TGG - Chr6:66868864-66868883 MsG0680033509.01.T01:intron 25.0%
!!! CCATTAAATTGTTGACTTAT+AGG - Chr6:66868958-66868977 MsG0680033509.01.T01:intron 25.0%
!!! TTTGATAATTTTCAAATGGG+AGG - Chr6:66868806-66868825 MsG0680033509.01.T01:intron 25.0%
AAAAAGTTATCTACTCACCA+AGG + Chr6:66869720-66869739 None:intergenic 30.0%
AAAGAAAACTTGGATTTGGA+CGG - Chr6:66869872-66869891 MsG0680033509.01.T01:intron 30.0%
AATTACAAAGTAGGGGAAAA+TGG + Chr6:66869550-66869569 None:intergenic 30.0%
ATCAATTAGGCCTTCTTTAT+AGG + Chr6:66870293-66870312 None:intergenic 30.0%
ATGGACATAACCTTCAATTT+AGG + Chr6:66870027-66870046 None:intergenic 30.0%
ATTAGGAACCTACTGTAATT+AGG + Chr6:66868691-66868710 None:intergenic 30.0%
ATTTCAGAGGCTAAAATCTT+GGG - Chr6:66869220-66869239 MsG0680033509.01.T01:intron 30.0%
CCATTATACATGTCAATTCA+AGG - Chr6:66870470-66870489 MsG0680033509.01.T01:intron 30.0%
CCTTGAATTGACATGTATAA+TGG + Chr6:66870473-66870492 None:intergenic 30.0%
CTAACTCTTCTAAACAACAA+GGG + Chr6:66870659-66870678 None:intergenic 30.0%
GTCAGATAGTCAAATCAATT+GGG - Chr6:66870737-66870756 MsG0680033509.01.T01:CDS 30.0%
GTGATTCATAAGCATGTTAA+AGG + Chr6:66869924-66869943 None:intergenic 30.0%
TAACATGCTTATGAATCACT+AGG - Chr6:66869925-66869944 MsG0680033509.01.T01:intron 30.0%
TAACTCTTCTAAACAACAAG+GGG + Chr6:66870658-66870677 None:intergenic 30.0%
TAAGTCAACAATTTAATGGC+AGG + Chr6:66868957-66868976 None:intergenic 30.0%
TCAATTAGGCCTTCTTTATA+GGG + Chr6:66870292-66870311 None:intergenic 30.0%
TCAGATAGTCAAATCAATTG+GGG - Chr6:66870738-66870757 MsG0680033509.01.T01:CDS 30.0%
TCATTAGGAAGGAATGTAAA+TGG + Chr6:66868901-66868920 None:intergenic 30.0%
TCTAACTCTTCTAAACAACA+AGG + Chr6:66870660-66870679 None:intergenic 30.0%
TGGACATAACCTTCAATTTA+GGG + Chr6:66870026-66870045 None:intergenic 30.0%
TGTCAGATAGTCAAATCAAT+TGG - Chr6:66870736-66870755 MsG0680033509.01.T01:CDS 30.0%
TTAAATGGCCTAATTACAGT+AGG - Chr6:66868680-66868699 MsG0680033509.01.T01:intron 30.0%
TTTAATAATGAGTCAGGAGA+CGG - Chr6:66869814-66869833 MsG0680033509.01.T01:CDS 30.0%
! AATTTGAACTGATAGTGTCT+TGG - Chr6:66869421-66869440 MsG0680033509.01.T01:CDS 30.0%
! ATTTTCATGCATGATTTCAG+AGG - Chr6:66869207-66869226 MsG0680033509.01.T01:intron 30.0%
!! AAAGCATTGAGTTTGTGTAA+AGG + Chr6:66869056-66869075 None:intergenic 30.0%
!!! ATCATCAGGGTTTTAAATTG+GGG - Chr6:66870082-66870101 MsG0680033509.01.T01:CDS 30.0%
!!! CATCATCAGGGTTTTAAATT+GGG - Chr6:66870081-66870100 MsG0680033509.01.T01:CDS 30.0%
!!! TCATCATCAGGGTTTTAAAT+TGG - Chr6:66870080-66870099 MsG0680033509.01.T01:CDS 30.0%
AAGAAAACTTGGATTTGGAC+GGG - Chr6:66869873-66869892 MsG0680033509.01.T01:intron 35.0%
AATGCAAATTCAGTTGGGTT+AGG - Chr6:66869984-66870003 MsG0680033509.01.T01:intron 35.0%
AGGTTATGTCCATGAAAGAT+AGG - Chr6:66870034-66870053 MsG0680033509.01.T01:CDS 35.0%
AGTGAGTAATATGCCTATGA+AGG + Chr6:66870627-66870646 None:intergenic 35.0%
CAAAGATGACTTCATTAGGA+AGG + Chr6:66868912-66868931 None:intergenic 35.0%
CAAGAGGTCACAAAATCTAA+TGG - Chr6:66868553-66868572 MsG0680033509.01.T01:CDS 35.0%
CAATGGAGTGAAGATCAATT+AGG + Chr6:66870306-66870325 None:intergenic 35.0%
GATTTCAGAGGCTAAAATCT+TGG - Chr6:66869219-66869238 MsG0680033509.01.T01:intron 35.0%
GCAGACATTGAAAGAAAACT+TGG - Chr6:66869862-66869881 MsG0680033509.01.T01:intron 35.0%
GCATCAAAGATGACTTCATT+AGG + Chr6:66868916-66868935 None:intergenic 35.0%
GGATGGGTGAAAAAAAGAAA+AGG - Chr6:66870799-66870818 MsG0680033509.01.T01:CDS 35.0%
GGTTAGGTTTATTCATGTTG+CGG - Chr6:66870000-66870019 MsG0680033509.01.T01:intron 35.0%
TCGCTTAAACAATGGAAAAG+AGG - Chr6:66868983-66869002 MsG0680033509.01.T01:intron 35.0%
TTTCAGAGGCTAAAATCTTG+GGG - Chr6:66869221-66869240 MsG0680033509.01.T01:intron 35.0%
! AAATTTGCTTCTGAAGGACT+TGG - Chr6:66870778-66870797 MsG0680033509.01.T01:CDS 35.0%
! ATACTTGGTCAGATTTTCAC+TGG + Chr6:66870512-66870531 None:intergenic 35.0%
! ATTTAAAACCCTGGTCATTG+TGG - Chr6:66870230-66870249 MsG0680033509.01.T01:intron 35.0%
! GGTAATCCTGCAAACAATTT+TGG + Chr6:66868640-66868659 None:intergenic 35.0%
! GTATTTTCGCATGACTTACA+AGG - Chr6:66869784-66869803 MsG0680033509.01.T01:intron 35.0%
! TGCTTATGCTTTTGCATCAA+TGG - Chr6:66870698-66870717 MsG0680033509.01.T01:CDS 35.0%
!! AATATCAGGAAAGCATTCAC+AGG + Chr6:66870404-66870423 None:intergenic 35.0%
ACGTCTACTCAGTTGAACAA+GGG - Chr6:66868588-66868607 MsG0680033509.01.T01:intron 40.0%
ATGAAGTCATCTTTGATGCC+TGG - Chr6:66868917-66868936 MsG0680033509.01.T01:intron 40.0%
CAAGGGATTATCCCTATAAC+TGG - Chr6:66868605-66868624 MsG0680033509.01.T01:intron 40.0%
CAGAGAATCACGGTTAAATG+TGG - Chr6:66870128-66870147 MsG0680033509.01.T01:CDS 40.0%
CTTGACATTGCAGAGAATCA+CGG - Chr6:66870118-66870137 MsG0680033509.01.T01:CDS 40.0%
CTTGGCGTCTCATGTTTAAA+CGG - Chr6:66869250-66869269 MsG0680033509.01.T01:intron 40.0%
GAATGGCCAAAATTGTTTGC+AGG - Chr6:66868631-66868650 MsG0680033509.01.T01:intron 40.0%
TACAGAAAACGACATCACTG+CGG - Chr6:66869167-66869186 MsG0680033509.01.T01:intron 40.0%
TACGTCTACTCAGTTGAACA+AGG - Chr6:66868587-66868606 MsG0680033509.01.T01:intron 40.0%
TAGAAGGAGTCTTAGTCAAG+AGG - Chr6:66868537-66868556 MsG0680033509.01.T01:CDS 40.0%
TATCCAGGAGTTCACAGATT+AGG - Chr6:66870579-66870598 MsG0680033509.01.T01:intron 40.0%
TCGTTCAATAATCACCATGC+AGG - Chr6:66869123-66869142 MsG0680033509.01.T01:intron 40.0%
TCTGAAGATCACAGGATACT+TGG + Chr6:66870527-66870546 None:intergenic 40.0%
TCTTGACTAAGACTCCTTCT+AGG + Chr6:66868538-66868557 None:intergenic 40.0%
TGTCAAGATCTTGGTTAGAG+TGG - Chr6:66868471-66868490 MsG0680033509.01.T01:CDS 40.0%
TTGGCGTCTCATGTTTAAAC+GGG - Chr6:66869251-66869270 MsG0680033509.01.T01:intron 40.0%
! CGGACACTTATTTAAAACCC+TGG - Chr6:66870221-66870240 MsG0680033509.01.T01:intron 40.0%
! GCTTCTTCAATCCTGGATTT+TGG + Chr6:66869453-66869472 None:intergenic 40.0%
! GCTTTTGCATCAATGGTTGT+AGG - Chr6:66870705-66870724 MsG0680033509.01.T01:CDS 40.0%
! TATAGGCGTCGCTTAAACAA+TGG - Chr6:66868975-66868994 MsG0680033509.01.T01:intron 40.0%
!! ATCCCTATAACTGGTTCGAA+TGG - Chr6:66868614-66868633 MsG0680033509.01.T01:intron 40.0%
!! CATGTCAATTCAAGGGTACT+AGG - Chr6:66870478-66870497 MsG0680033509.01.T01:intron 40.0%
!! CTTGGTCTTTTCCAAAATCC+AGG - Chr6:66869439-66869458 MsG0680033509.01.T01:CDS 40.0%
!! TTCTTTTTGAGCTACCTGCA+TGG + Chr6:66869140-66869159 None:intergenic 40.0%
AAAATCTTGGGGATGGCTCT+TGG - Chr6:66869232-66869251 MsG0680033509.01.T01:intron 45.0%
AATAATGAGTCAGGAGACGG+TGG - Chr6:66869817-66869836 MsG0680033509.01.T01:CDS 45.0%
AGAGGCTAAAATCTTGGGGA+TGG - Chr6:66869225-66869244 MsG0680033509.01.T01:intron 45.0%
AGCTTCTGCTTCTTCAATCC+TGG + Chr6:66869460-66869479 None:intergenic 45.0%
AGGAGTTCACAGATTAGGTC+CGG - Chr6:66870584-66870603 MsG0680033509.01.T01:intron 45.0%
ATAGTCACTGCCAAGAAGCA+AGG + Chr6:66870559-66870578 None:intergenic 45.0%
ATGTTGCGGCCCTAAATTGA+AGG - Chr6:66870014-66870033 MsG0680033509.01.T01:intron 45.0%
CAGGAATTGATCCATCTACC+AGG + Chr6:66868938-66868957 None:intergenic 45.0%
CATCTTTGATGCCTGGTAGA+TGG - Chr6:66868924-66868943 MsG0680033509.01.T01:intron 45.0%
CGTGATTCTCTGCAATGTCA+AGG + Chr6:66870120-66870139 None:intergenic 45.0%
CTTGGCAGTGACTATTATCC+AGG - Chr6:66870564-66870583 MsG0680033509.01.T01:intron 45.0%
GATTACCTTGATAGAGCCAC+AGG - Chr6:66868653-66868672 MsG0680033509.01.T01:intron 45.0%
GGCCATTCGAACCAGTTATA+GGG + Chr6:66868619-66868638 None:intergenic 45.0%
TCCCTTGCACATCATCATCA+GGG - Chr6:66870069-66870088 MsG0680033509.01.T01:CDS 45.0%
TGCATGAAAATTCAGACCCC+AGG + Chr6:66869198-66869217 None:intergenic 45.0%
TGGCCATTCGAACCAGTTAT+AGG + Chr6:66868620-66868639 None:intergenic 45.0%
TTATGCCGCATCCTAGATCA+TGG - Chr6:66870195-66870214 MsG0680033509.01.T01:intron 45.0%
TTCCCTTGCACATCATCATC+AGG - Chr6:66870068-66870087 MsG0680033509.01.T01:CDS 45.0%
! AACCCTGATGATGATGTGCA+AGG + Chr6:66870074-66870093 None:intergenic 45.0%
! ACCCTGATGATGATGTGCAA+GGG + Chr6:66870073-66870092 None:intergenic 45.0%
! TGCTTCTGAAGGACTTGGAT+GGG - Chr6:66870783-66870802 MsG0680033509.01.T01:CDS 45.0%
! TTGCTTCTGAAGGACTTGGA+TGG - Chr6:66870782-66870801 MsG0680033509.01.T01:CDS 45.0%
AAACCCTGGTCATTGTGGAG+AGG - Chr6:66870235-66870254 MsG0680033509.01.T01:intron 50.0%
ACATCACTGCGGAGAAACCT+GGG - Chr6:66869178-66869197 MsG0680033509.01.T01:intron 50.0%
ACTGCCAAGAAGCAAGGAGA+GGG + Chr6:66870553-66870572 None:intergenic 50.0%
ATTGCCCTCTCCTTGCTTCT+TGG - Chr6:66870546-66870565 MsG0680033509.01.T01:intron 50.0%
CAGCAACGTGATCAAGACTG+AGG + Chr6:66870154-66870173 None:intergenic 50.0%
GAGGGCAATCTGAAGATCAC+AGG + Chr6:66870535-66870554 None:intergenic 50.0%
GGACCTAATCTGTGAACTCC+TGG + Chr6:66870585-66870604 None:intergenic 50.0%
GTGTACAGGGATATCTGCCT+TGG - Chr6:66869700-66869719 MsG0680033509.01.T01:CDS 50.0%
TAGGCCATTTAACGCTCCTG+TGG + Chr6:66868672-66868691 None:intergenic 50.0%
TCCTGAGACGTGTCCTTCAT+AGG - Chr6:66870611-66870630 MsG0680033509.01.T01:CDS 50.0%
TGGATTTGGACGGGACTCAT+TGG - Chr6:66869882-66869901 MsG0680033509.01.T01:intron 50.0%
TGGTGCAAGGACAGTGTACA+GGG - Chr6:66869687-66869706 MsG0680033509.01.T01:CDS 50.0%
TTGGTGCAAGGACAGTGTAC+AGG - Chr6:66869686-66869705 MsG0680033509.01.T01:CDS 50.0%
!! AGAGCCACAGGAGCGTTAAA+TGG - Chr6:66868665-66868684 MsG0680033509.01.T01:intron 50.0%
AGTGTCCGCGACCATGATCT+AGG + Chr6:66870209-66870228 None:intergenic 55.0%
CACTGCCAAGAAGCAAGGAG+AGG + Chr6:66870554-66870573 None:intergenic 55.0%
CATCACTGCGGAGAAACCTG+GGG - Chr6:66869179-66869198 MsG0680033509.01.T01:intron 55.0%
CGCATCCTAGATCATGGTCG+CGG - Chr6:66870201-66870220 MsG0680033509.01.T01:intron 55.0%
GACATCACTGCGGAGAAACC+TGG - Chr6:66869177-66869196 MsG0680033509.01.T01:intron 55.0%
GAGCCTCTCCACAATGACCA+GGG + Chr6:66870241-66870260 None:intergenic 55.0%
GCCTATGAAGGACACGTCTC+AGG + Chr6:66870615-66870634 None:intergenic 55.0%
GGACACGTCTCAGGACTTAC+CGG + Chr6:66870606-66870625 None:intergenic 55.0%
GGATACGCACTCTGACTCCT+AGG - Chr6:66868492-66868511 MsG0680033509.01.T01:CDS 55.0%
TGAGCCTCTCCACAATGACC+AGG + Chr6:66870242-66870261 None:intergenic 55.0%
! ACGCTCCTGTGGCTCTATCA+AGG + Chr6:66868661-66868680 None:intergenic 55.0%
! CGCGACCATGATCTAGGATG+CGG + Chr6:66870203-66870222 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 66868470 66870831 66868470 ID=MsG0680033509.01;Name=MsG0680033509.01
Chr6 mRNA 66868470 66870831 66868470 ID=MsG0680033509.01.T01;Parent=MsG0680033509.01;Name=MsG0680033509.01.T01;_AED=0.61;_eAED=0.63;_QI=0|0.33|0.42|0.85|0.33|0.42|7|0|267
Chr6 exon 66870600 66870831 66870600 ID=MsG0680033509.01.T01:exon:3256;Parent=MsG0680033509.01.T01
Chr6 exon 66870297 66870358 66870297 ID=MsG0680033509.01.T01:exon:3257;Parent=MsG0680033509.01.T01
Chr6 exon 66870029 66870156 66870029 ID=MsG0680033509.01.T01:exon:3258;Parent=MsG0680033509.01.T01
Chr6 exon 66869789 66869840 66869789 ID=MsG0680033509.01.T01:exon:3259;Parent=MsG0680033509.01.T01
Chr6 exon 66869580 66869726 66869580 ID=MsG0680033509.01.T01:exon:3260;Parent=MsG0680033509.01.T01
Chr6 exon 66869398 66869475 66869398 ID=MsG0680033509.01.T01:exon:3261;Parent=MsG0680033509.01.T01
Chr6 exon 66868470 66868574 66868470 ID=MsG0680033509.01.T01:exon:3262;Parent=MsG0680033509.01.T01
Chr6 CDS 66870600 66870831 66870600 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Chr6 CDS 66870297 66870358 66870297 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Chr6 CDS 66870029 66870156 66870029 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Chr6 CDS 66869789 66869840 66869789 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Chr6 CDS 66869580 66869726 66869580 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Chr6 CDS 66869398 66869475 66869398 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Chr6 CDS 66868470 66868574 66868470 ID=MsG0680033509.01.T01:cds;Parent=MsG0680033509.01.T01
Gene Sequence

>MsG0680033509.01.T01

ATGTCAAGATCTTGGTTAGAGTGGATACGCACTCTGACTCCTAGGAACATAAATCAAATTCAATTCCTAGAAGGAGTCTTAGTCAAGAGGTCACAAAATCTAATGGATAAATTCTCATACGTCTACTCAGTTGAACAAGGGATTATCCCTATAACTGGTTCGAATGGCCAAAATTGTTTGCAGGATTACCTTGATAGAGCCACAGGAGCGTTAAATGGCCTAATTACAGTAGATGGATCAATTCCTGCCATTAAATTGTTGACTTATAGGCGTCGCTTAAACAATGGAAAAGAGGTAGCTCAAAAAGAAACTAACTTACAGAAAACGACATCACTGCGGAGAAACCTGGGGTCTGAATTTTCATGCATGATTTCAGAGGCTAAAATCTTGGGGATGGCTCTTGGCGTCTCATGTTTAAACGGGATTGAAGAAGCAGAAGCTCAGTGTGCTTTGTTGAACTCTGAATCGTTATGTTTATTTTTGGTAAACTTGTGTTATTGTTGTTCGCAAATCATCATGATCGCTGTCATTCATTTTGAAAATTATAGGATGGATGTTTCAGTTCAGATTCAGATATATTTCTTTTTGGTGCAAGGACAGTGTACAGGGATATCTGCCTTGTCAGGAGACGGTGGATATGTTGTATGTTATGAGATGGCAGACATTGAAAGAAAACTTGGATTTGGACGGGACTCATTGGATTCAGCTTGTCAGATAGTCAAATCAATTGGGGACAAATTTGTTCTAAAAAAATTTGCTTCTGAAGGACTTGGATGGGTGAAAAAAAGAAAAGGTCTACCCTAA
Protein sequence

>MsG0680033509.01.T01

MSRSWLEWIRTLTPRNINQIQFLEGVLVKRSQNLMDKFSYVYSVEQGIIPITGSNGQNCLQDYLDRATGALNGLITVDGSIPAIKLLTYRRRLNNGKEVAQKETNLQKTTSLRRNLGSEFSCMISEAKILGMALGVSCLNGIEEAEAQCALLNSESLCLFLVNLCYCCSQIIMIAVIHFENYRMDVSVQIQIYFFLVQGQCTGISALSGDGGYVVCYEMADIERKLGFGRDSLDSACQIVKSIGDKFVLKKFASEGLGWVKKRKGLP*