Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034376.01.T01 | RHN52140.1 | 95.335 | 343 | 15 | 1 | 1 | 342 | 349 | 691 | 0 | 685 |
| MsG0680034376.01.T01 | RHN52140.1 | 70.175 | 342 | 98 | 1 | 1 | 342 | 1 | 338 | 7.29E-178 | 520 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034376.01.T01 | Q9LT10 | 57.817 | 339 | 124 | 6 | 2 | 336 | 5 | 328 | 8.37E-145 | 415 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680034376.01 | MsG0780040621.01 | PPI |
| MsG0180004920.01 | MsG0680034376.01 | PPI |
| MsG0180004920.01 | MsG0680034376.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034376.01.T01 | MTR_6g065120 | 95.335 | 343 | 15 | 1 | 1 | 342 | 1 | 343 | 0.0 | 682 |
| MsG0680034376.01.T01 | MTR_6g065270 | 78.107 | 338 | 70 | 1 | 9 | 342 | 6 | 343 | 0.0 | 564 |
| MsG0680034376.01.T01 | MTR_6g065370 | 76.696 | 339 | 79 | 0 | 3 | 341 | 2 | 340 | 0.0 | 560 |
| MsG0680034376.01.T01 | MTR_6g065400 | 76.696 | 339 | 79 | 0 | 3 | 341 | 2 | 340 | 0.0 | 558 |
| MsG0680034376.01.T01 | MTR_6g065110 | 70.588 | 340 | 99 | 1 | 1 | 339 | 1 | 340 | 0.0 | 523 |
| MsG0680034376.01.T01 | MTR_6g065340 | 74.412 | 340 | 71 | 1 | 2 | 341 | 12 | 335 | 0.0 | 521 |
| MsG0680034376.01.T01 | MTR_6g065430 | 59.819 | 331 | 124 | 2 | 8 | 336 | 6 | 329 | 2.83e-148 | 421 |
| MsG0680034376.01.T01 | MTR_1g082210 | 37.982 | 337 | 202 | 2 | 3 | 335 | 8 | 341 | 2.70e-80 | 248 |
| MsG0680034376.01.T01 | MTR_7g093950 | 37.463 | 339 | 203 | 4 | 5 | 335 | 10 | 347 | 2.75e-78 | 243 |
| MsG0680034376.01.T01 | MTR_1g089310 | 38.393 | 336 | 196 | 5 | 5 | 334 | 10 | 340 | 2.84e-75 | 236 |
| MsG0680034376.01.T01 | MTR_1g089310 | 38.393 | 336 | 196 | 5 | 5 | 334 | 26 | 356 | 3.66e-75 | 236 |
| MsG0680034376.01.T01 | MTR_6g464280 | 36.452 | 310 | 182 | 7 | 33 | 335 | 24 | 325 | 2.19e-54 | 181 |
| MsG0680034376.01.T01 | MTR_8g035520 | 35.117 | 299 | 172 | 7 | 50 | 337 | 40 | 327 | 4.46e-48 | 165 |
| MsG0680034376.01.T01 | MTR_6g464510 | 35.000 | 280 | 160 | 7 | 30 | 299 | 25 | 292 | 6.35e-48 | 164 |
| MsG0680034376.01.T01 | MTR_8g035540 | 33.968 | 315 | 185 | 7 | 32 | 334 | 13 | 316 | 9.54e-48 | 164 |
| MsG0680034376.01.T01 | MTR_6g464320 | 34.277 | 318 | 194 | 7 | 25 | 335 | 17 | 326 | 5.24e-47 | 162 |
| MsG0680034376.01.T01 | MTR_7g406880 | 32.343 | 303 | 178 | 8 | 56 | 336 | 54 | 351 | 2.16e-46 | 161 |
| MsG0680034376.01.T01 | MTR_8g035500 | 32.372 | 312 | 190 | 7 | 33 | 334 | 17 | 317 | 1.20e-45 | 158 |
| MsG0680034376.01.T01 | MTR_1g102160 | 33.815 | 346 | 188 | 12 | 3 | 334 | 7 | 325 | 5.87e-45 | 157 |
| MsG0680034376.01.T01 | MTR_4g050140 | 32.982 | 285 | 173 | 4 | 61 | 336 | 42 | 317 | 2.79e-43 | 152 |
| MsG0680034376.01.T01 | MTR_0007s0520 | 37.668 | 223 | 121 | 5 | 49 | 265 | 39 | 249 | 1.29e-41 | 148 |
| MsG0680034376.01.T01 | MTR_3g012440 | 31.928 | 332 | 191 | 8 | 32 | 336 | 19 | 342 | 9.25e-41 | 146 |
| MsG0680034376.01.T01 | MTR_1g012430 | 31.290 | 310 | 193 | 7 | 32 | 336 | 15 | 309 | 1.57e-40 | 144 |
| MsG0680034376.01.T01 | MTR_1g111280 | 33.003 | 303 | 170 | 10 | 49 | 334 | 52 | 338 | 1.77e-40 | 145 |
| MsG0680034376.01.T01 | MTR_1g102170 | 35.193 | 233 | 127 | 7 | 33 | 257 | 43 | 259 | 4.86e-39 | 141 |
| MsG0680034376.01.T01 | MTR_6g464120 | 34.454 | 238 | 136 | 6 | 33 | 265 | 29 | 251 | 5.61e-39 | 145 |
| MsG0680034376.01.T01 | MTR_4g086510 | 33.108 | 296 | 176 | 8 | 50 | 335 | 35 | 318 | 8.93e-39 | 140 |
| MsG0680034376.01.T01 | MTR_7g107040 | 29.825 | 342 | 203 | 8 | 11 | 339 | 4 | 321 | 6.24e-37 | 135 |
| MsG0680034376.01.T01 | MTR_4g086480 | 31.633 | 294 | 178 | 6 | 50 | 334 | 39 | 318 | 6.77e-37 | 135 |
| MsG0680034376.01.T01 | MTR_7g056207 | 30.619 | 307 | 186 | 8 | 44 | 335 | 56 | 350 | 1.74e-35 | 132 |
| MsG0680034376.01.T01 | MTR_4g086540 | 29.180 | 305 | 182 | 8 | 39 | 338 | 33 | 308 | 1.41e-33 | 126 |
| MsG0680034376.01.T01 | MTR_4g086470 | 31.081 | 296 | 181 | 6 | 50 | 336 | 38 | 319 | 1.56e-32 | 124 |
| MsG0680034376.01.T01 | MTR_3g101420 | 28.667 | 300 | 184 | 7 | 50 | 336 | 34 | 316 | 1.75e-30 | 118 |
| MsG0680034376.01.T01 | MTR_6g022710 | 29.787 | 282 | 158 | 5 | 90 | 333 | 154 | 433 | 6.19e-30 | 119 |
| MsG0680034376.01.T01 | MTR_1g105020 | 28.750 | 320 | 192 | 11 | 33 | 334 | 21 | 322 | 2.16e-29 | 115 |
| MsG0680034376.01.T01 | MTR_5g005850 | 27.517 | 298 | 185 | 9 | 54 | 334 | 45 | 328 | 3.28e-27 | 109 |
| MsG0680034376.01.T01 | MTR_3g450680 | 26.871 | 294 | 187 | 8 | 54 | 332 | 48 | 328 | 7.85e-26 | 105 |
| MsG0680034376.01.T01 | MTR_5g005920 | 26.073 | 303 | 189 | 10 | 54 | 336 | 45 | 332 | 2.80e-25 | 104 |
| MsG0680034376.01.T01 | MTR_5g005900 | 27.385 | 325 | 199 | 13 | 32 | 336 | 21 | 328 | 1.05e-24 | 102 |
| MsG0680034376.01.T01 | MTR_6g065470 | 36.257 | 171 | 77 | 3 | 8 | 160 | 6 | 162 | 2.16e-24 | 97.8 |
| MsG0680034376.01.T01 | MTR_8g040860 | 25.862 | 290 | 186 | 7 | 54 | 329 | 48 | 322 | 8.61e-24 | 100 |
| MsG0680034376.01.T01 | MTR_7g051020 | 28.483 | 323 | 194 | 11 | 32 | 336 | 24 | 327 | 1.44e-21 | 94.0 |
| MsG0680034376.01.T01 | MTR_1g104930 | 29.393 | 313 | 185 | 11 | 44 | 336 | 28 | 324 | 2.53e-21 | 93.2 |
| MsG0680034376.01.T01 | MTR_1g104933 | 29.655 | 290 | 174 | 9 | 60 | 336 | 46 | 318 | 4.31e-20 | 89.7 |
| MsG0680034376.01.T01 | MTR_0007s0480 | 41.758 | 91 | 47 | 2 | 172 | 257 | 1 | 90 | 5.73e-16 | 75.1 |
| MsG0680034376.01.T01 | MTR_6g065480 | 51.724 | 58 | 27 | 1 | 234 | 291 | 1 | 57 | 8.04e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034376.01.T01 | AT5G23530 | 57.817 | 339 | 124 | 6 | 2 | 336 | 5 | 328 | 8.53e-146 | 415 |
| MsG0680034376.01.T01 | AT5G27320 | 38.806 | 335 | 195 | 3 | 6 | 335 | 11 | 340 | 2.04e-81 | 251 |
| MsG0680034376.01.T01 | AT3G63010 | 37.026 | 343 | 206 | 4 | 5 | 341 | 10 | 348 | 2.70e-75 | 236 |
| MsG0680034376.01.T01 | AT3G05120 | 35.821 | 335 | 207 | 2 | 6 | 335 | 11 | 342 | 1.58e-72 | 229 |
| MsG0680034376.01.T01 | AT5G62180 | 35.783 | 313 | 186 | 9 | 33 | 337 | 21 | 326 | 7.83e-54 | 180 |
| MsG0680034376.01.T01 | AT2G45610 | 33.013 | 312 | 191 | 6 | 33 | 335 | 22 | 324 | 7.83e-46 | 159 |
| MsG0680034376.01.T01 | AT2G45600 | 31.973 | 294 | 176 | 7 | 60 | 340 | 39 | 321 | 7.64e-44 | 154 |
| MsG0680034376.01.T01 | AT1G68620 | 32.517 | 286 | 172 | 7 | 57 | 334 | 57 | 329 | 5.29e-42 | 149 |
| MsG0680034376.01.T01 | AT5G16080 | 31.788 | 302 | 179 | 8 | 49 | 339 | 54 | 339 | 1.13e-41 | 149 |
| MsG0680034376.01.T01 | AT5G06570 | 35.913 | 323 | 176 | 11 | 32 | 338 | 22 | 329 | 4.75e-41 | 146 |
| MsG0680034376.01.T01 | AT5G06570 | 35.913 | 323 | 176 | 11 | 32 | 338 | 22 | 329 | 4.75e-41 | 146 |
| MsG0680034376.01.T01 | AT5G06570 | 35.913 | 323 | 176 | 11 | 32 | 338 | 37 | 344 | 6.05e-41 | 147 |
| MsG0680034376.01.T01 | AT5G06570 | 35.913 | 323 | 176 | 11 | 32 | 338 | 37 | 344 | 6.05e-41 | 147 |
| MsG0680034376.01.T01 | AT1G47480 | 29.179 | 329 | 209 | 9 | 14 | 338 | 5 | 313 | 1.65e-37 | 137 |
| MsG0680034376.01.T01 | AT1G19190 | 30.100 | 299 | 182 | 9 | 50 | 336 | 32 | 315 | 3.09e-34 | 128 |
| MsG0680034376.01.T01 | AT3G48700 | 29.316 | 307 | 182 | 8 | 50 | 334 | 32 | 325 | 4.88e-33 | 125 |
| MsG0680034376.01.T01 | AT3G48690 | 31.438 | 299 | 181 | 9 | 50 | 334 | 32 | 320 | 9.28e-32 | 122 |
| MsG0680034376.01.T01 | AT5G14310 | 30.584 | 291 | 148 | 6 | 96 | 335 | 151 | 438 | 5.52e-31 | 122 |
| MsG0680034376.01.T01 | AT3G27320 | 27.457 | 346 | 171 | 7 | 48 | 314 | 48 | 392 | 7.61e-30 | 118 |
| MsG0680034376.01.T01 | AT2G03550 | 29.530 | 298 | 182 | 8 | 50 | 336 | 32 | 312 | 1.19e-29 | 115 |
| MsG0680034376.01.T01 | AT3G27320 | 30.742 | 283 | 146 | 7 | 73 | 314 | 151 | 424 | 8.36e-29 | 116 |
| MsG0680034376.01.T01 | AT1G49640 | 31.373 | 255 | 159 | 9 | 31 | 282 | 17 | 258 | 1.83e-28 | 112 |
| MsG0680034376.01.T01 | AT1G49650 | 30.859 | 256 | 160 | 7 | 54 | 302 | 92 | 337 | 1.20e-24 | 103 |
| MsG0680034376.01.T01 | AT1G49660 | 28.685 | 251 | 165 | 4 | 54 | 298 | 36 | 278 | 3.83e-24 | 101 |
Find 106 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCGGAAGGGTGGCGGTTT+TGG | 0.181057 | 6:-86550545 | None:intergenic |
| AGACAGTGCCGGTGCAAATT+TGG | 0.247856 | 6:+86550810 | MsG0680034376.01.T01:CDS |
| TCTTGACACGTCAGCATATT+CGG | 0.273859 | 6:-86550773 | None:intergenic |
| TGCGGAGGATTTGTCGGGTT+TGG | 0.280062 | 6:+86551059 | MsG0680034376.01.T01:CDS |
| GGCGTTAACGATGACGTCTT+TGG | 0.299465 | 6:-86550452 | None:intergenic |
| TTGGTGGAAACGCCGTTAAC+AGG | 0.301029 | 6:-86550433 | None:intergenic |
| TTTCCAAGGGAGGAAGGGTT+TGG | 0.310053 | 6:-86550293 | None:intergenic |
| AGGAAGGGTTTGGGTTTGTT+TGG | 0.313143 | 6:-86550283 | None:intergenic |
| GCGTCATAAGCAAATGAATC+AGG | 0.346502 | 6:-86550607 | None:intergenic |
| GGCACATCACGTTGCGGTTA+GGG | 0.350643 | 6:+86550831 | MsG0680034376.01.T01:CDS |
| TTTCCTGATTTGCCTGAGTC+TGG | 0.357988 | 6:+86551222 | MsG0680034376.01.T01:CDS |
| CACCAAAACCGCCACCCTTC+CGG | 0.359575 | 6:+86550543 | MsG0680034376.01.T01:CDS |
| GGTTTGATCCTTTAAATGAT+TGG | 0.359729 | 6:+86551112 | MsG0680034376.01.T01:CDS |
| TTCCAAGGGAGGAAGGGTTT+GGG | 0.364912 | 6:-86550292 | None:intergenic |
| TGGCACATCACGTTGCGGTT+AGG | 0.366316 | 6:+86550830 | MsG0680034376.01.T01:CDS |
| GCGGTTAGGGTTTGCAAAGC+GGG | 0.370441 | 6:+86550844 | MsG0680034376.01.T01:CDS |
| GGCAAAAGAGGTATTATGAT+TGG | 0.376841 | 6:+86551133 | MsG0680034376.01.T01:CDS |
| CCGTTAACAGGGATGGCGTT+AGG | 0.383299 | 6:-86550421 | None:intergenic |
| CTCCGAGAAATCCGGGTTAT+CGG | 0.383375 | 6:+86550868 | MsG0680034376.01.T01:CDS |
| AAGCGGGGCTCCGAGAAATC+CGG | 0.396785 | 6:+86550860 | MsG0680034376.01.T01:CDS |
| TGACCAGACTCAGGCAAATC+AGG | 0.397962 | 6:-86551225 | None:intergenic |
| GCCGAATGCGGAGGATTTGT+CGG | 0.408259 | 6:+86551053 | MsG0680034376.01.T01:CDS |
| AACCCAAACCCTTCCTCCCT+TGG | 0.409062 | 6:+86550290 | MsG0680034376.01.T01:CDS |
| CGTTAACAGGGATGGCGTTA+GGG | 0.417430 | 6:-86550420 | None:intergenic |
| GGCGGAATTGACGGTGGTAG+TGG | 0.419693 | 6:-86550506 | None:intergenic |
| CCTAACGCCATCCCTGTTAA+CGG | 0.424067 | 6:+86550421 | MsG0680034376.01.T01:CDS |
| AAATGAATCAGGGGTGAGAT+AGG | 0.429334 | 6:-86550596 | None:intergenic |
| CTTGACACGTCAGCATATTC+GGG | 0.435567 | 6:-86550772 | None:intergenic |
| CCGAATGCGGAGGATTTGTC+GGG | 0.436284 | 6:+86551054 | MsG0680034376.01.T01:CDS |
| GAGGGGTCGGATCGCGATCA+TGG | 0.436406 | 6:+86551012 | MsG0680034376.01.T01:CDS |
| ATTTGCACCGGCACTGTCTC+CGG | 0.436925 | 6:-86550806 | None:intergenic |
| ACCCGATAACCCGGATTTCT+CGG | 0.437356 | 6:-86550870 | None:intergenic |
| AGAGGACGGAGGCGGAGATT+AGG | 0.439237 | 6:+86550926 | MsG0680034376.01.T01:CDS |
| CCCGACAAATCCTCCGCATT+CGG | 0.445071 | 6:-86551054 | None:intergenic |
| ATTATCTCCGGCGGAATTGA+CGG | 0.446061 | 6:-86550515 | None:intergenic |
| TCCGAGAAATCCGGGTTATC+GGG | 0.451778 | 6:+86550869 | MsG0680034376.01.T01:CDS |
| TTTGTCGGGTTTGGATTATC+CGG | 0.455467 | 6:+86551068 | MsG0680034376.01.T01:CDS |
| TGGGGAGGAGAGGACGGAGG+CGG | 0.459130 | 6:+86550918 | MsG0680034376.01.T01:CDS |
| CTTTAAATGATTGGCAAAAG+AGG | 0.459493 | 6:+86551121 | MsG0680034376.01.T01:CDS |
| CATGGGACGGTTAATGTATG+TGG | 0.476930 | 6:+86551030 | MsG0680034376.01.T01:CDS |
| TGGTGGGGAGGAGAGGACGG+AGG | 0.481621 | 6:+86550915 | MsG0680034376.01.T01:CDS |
| TTAACGCCGAAAATAACGTT+TGG | 0.482150 | 6:+86550467 | MsG0680034376.01.T01:CDS |
| CGCGATTTCCAAGGGAGGAA+GGG | 0.490910 | 6:-86550298 | None:intergenic |
| AGGGGTCGGATCGCGATCAT+GGG | 0.495092 | 6:+86551013 | MsG0680034376.01.T01:CDS |
| AGCGGGGCTCCGAGAAATCC+GGG | 0.496119 | 6:+86550861 | MsG0680034376.01.T01:CDS |
| TATGATTGGTTGAAGAAATG+TGG | 0.503360 | 6:+86551147 | MsG0680034376.01.T01:CDS |
| TGTCGATGGCGAGGACTGAT+TGG | 0.513850 | 6:+86550968 | MsG0680034376.01.T01:CDS |
| TGCGATGGATACCGATGCTC+CGG | 0.517437 | 6:-86550694 | None:intergenic |
| GCGCGATTTCCAAGGGAGGA+AGG | 0.523971 | 6:-86550299 | None:intergenic |
| ATATTTGGACGAGAATAAAA+CGG | 0.525878 | 6:+86550744 | MsG0680034376.01.T01:CDS |
| GGGATCACCGTTGGTGTCGA+TGG | 0.525934 | 6:+86550954 | MsG0680034376.01.T01:CDS |
| TTTGGTGTCGATATTATCTC+CGG | 0.526368 | 6:-86550527 | None:intergenic |
| GATCTAAGAAATTAAGAAAA+CGG | 0.534119 | 6:-86550387 | None:intergenic |
| ACCGATGCTCCGGCGTGTGA+CGG | 0.542887 | 6:-86550684 | None:intergenic |
| GCGGAATTGACGGTGGTAGT+GGG | 0.549165 | 6:-86550505 | None:intergenic |
| CGGTTAGGGTTTGCAAAGCG+GGG | 0.549323 | 6:+86550845 | MsG0680034376.01.T01:CDS |
| CGTCATAAGCAAATGAATCA+GGG | 0.550916 | 6:-86550606 | None:intergenic |
| TGATCGCGATCCGACCCCTC+CGG | 0.551831 | 6:-86551009 | None:intergenic |
| AATGATCACCGGAAGGGTGG+CGG | 0.554851 | 6:-86550551 | None:intergenic |
| TGGATACCAACCCGATAACC+CGG | 0.557499 | 6:-86550879 | None:intergenic |
| ATGATTGGTTGAAGAAATGT+GGG | 0.557528 | 6:+86551148 | MsG0680034376.01.T01:CDS |
| GTTAACGATGACGTCTTTGG+TGG | 0.557766 | 6:-86550449 | None:intergenic |
| ACCGTCACACGCCGGAGCAT+CGG | 0.559818 | 6:+86550683 | MsG0680034376.01.T01:CDS |
| TGCGGTTAGGGTTTGCAAAG+CGG | 0.563025 | 6:+86550843 | MsG0680034376.01.T01:CDS |
| CTGTTTGCCGAAGATTCTGC+CGG | 0.569619 | 6:+86550629 | MsG0680034376.01.T01:CDS |
| TGGCGAGGACTGATTGGATG+TGG | 0.571085 | 6:+86550974 | MsG0680034376.01.T01:CDS |
| ATTGAAATGCGCGATTTCCA+AGG | 0.574433 | 6:-86550307 | None:intergenic |
| TGGTGGAAACGCCGTTAACA+GGG | 0.577904 | 6:-86550432 | None:intergenic |
| CACCGTTGGTGTCGATGGCG+AGG | 0.578623 | 6:+86550959 | MsG0680034376.01.T01:CDS |
| CGGCGTGTGACGGTAGCTAA+CGG | 0.581365 | 6:-86550674 | None:intergenic |
| GATAGGTAAATCCACCTCCA+TGG | 0.586090 | 6:-86550579 | None:intergenic |
| TTCCTCGCCGGAGACAGTGC+CGG | 0.593734 | 6:+86550799 | MsG0680034376.01.T01:CDS |
| TGGTAGTGGGTGTGAAGAGA+CGG | 0.595217 | 6:-86550492 | None:intergenic |
| TCAGTTGATTATGCAAGTCA+AGG | 0.597008 | 6:+86551245 | MsG0680034376.01.T01:CDS |
| AGAAATCCGGGTTATCGGGT+TGG | 0.598480 | 6:+86550873 | MsG0680034376.01.T01:CDS |
| GACGCTTCACGTCGCTCTAA+CGG | 0.599989 | 6:+86550352 | MsG0680034376.01.T01:CDS |
| TTGAAATGCGCGATTTCCAA+GGG | 0.600416 | 6:-86550306 | None:intergenic |
| GTCGGATCGCGATCATGGGA+CGG | 0.601207 | 6:+86551017 | MsG0680034376.01.T01:CDS |
| GGAAACGCCGTTAACAGGGA+TGG | 0.608074 | 6:-86550428 | None:intergenic |
| GTGTCAAGATGTTTCCTCGC+CGG | 0.608393 | 6:+86550787 | MsG0680034376.01.T01:CDS |
| CCATGGAAGAAAATGATCAC+CGG | 0.609666 | 6:-86550562 | None:intergenic |
| TCTCCATCGTCGTATTGCGA+TGG | 0.613465 | 6:-86550709 | None:intergenic |
| ACTACCACCGTCAATTCCGC+CGG | 0.615288 | 6:+86550508 | MsG0680034376.01.T01:CDS |
| GTGATGTGCCAAATTTGCAC+CGG | 0.615902 | 6:-86550818 | None:intergenic |
| ATAATCAACTGACCAGACTC+AGG | 0.616321 | 6:-86551234 | None:intergenic |
| GTTAACAGGGATGGCGTTAG+GGG | 0.618052 | 6:-86550419 | None:intergenic |
| GGAAGAAAATGATCACCGGA+AGG | 0.622089 | 6:-86550558 | None:intergenic |
| GAAAATGATCACCGGAAGGG+TGG | 0.622802 | 6:-86550554 | None:intergenic |
| ATGGGACGGTTAATGTATGT+GGG | 0.623324 | 6:+86551031 | MsG0680034376.01.T01:CDS |
| TAATGTATGTGGGCCGAATG+CGG | 0.627741 | 6:+86551041 | MsG0680034376.01.T01:CDS |
| TAGGCTCGAGGGATCACCGT+TGG | 0.628129 | 6:+86550945 | MsG0680034376.01.T01:CDS |
| TATCCATCGCAATACGACGA+TGG | 0.630849 | 6:+86550706 | MsG0680034376.01.T01:CDS |
| AAATTTGGCACATCACGTTG+CGG | 0.634684 | 6:+86550825 | MsG0680034376.01.T01:CDS |
| GGAGGCGGAGATTAGGCTCG+AGG | 0.635168 | 6:+86550933 | MsG0680034376.01.T01:CDS |
| CACCGGCACTGTCTCCGGCG+AGG | 0.645978 | 6:-86550801 | None:intergenic |
| GGTGTCGATATTATCTCCGG+CGG | 0.650490 | 6:-86550524 | None:intergenic |
| GTCCTCGCCATCGACACCAA+CGG | 0.651929 | 6:-86550961 | None:intergenic |
| GAAGAAAATGATCACCGGAA+GGG | 0.667962 | 6:-86550557 | None:intergenic |
| GTCATAAGCAAATGAATCAG+GGG | 0.669245 | 6:-86550605 | None:intergenic |
| AAATGCGCGATTTCCAAGGG+AGG | 0.686074 | 6:-86550303 | None:intergenic |
| GAGGCGGAGATTAGGCTCGA+GGG | 0.689293 | 6:+86550934 | MsG0680034376.01.T01:CDS |
| TGTATGTGGGCCGAATGCGG+AGG | 0.703597 | 6:+86551044 | MsG0680034376.01.T01:CDS |
| ATCTCCGGCGGAATTGACGG+TGG | 0.707755 | 6:-86550512 | None:intergenic |
| CGTTAGCTACCGTCACACGC+CGG | 0.709567 | 6:+86550675 | MsG0680034376.01.T01:CDS |
| GTAGCTAACGGAGACAACAA+CGG | 0.725578 | 6:-86550662 | None:intergenic |
| CTAAGAAATTAAGAAAACGG+CGG | 0.730991 | 6:-86550384 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATCTAAGAAATTAAGAAAA+CGG | - | Chr6:86550390-86550409 | None:intergenic | 20.0% |
| !!! | AATATAAAAAGCATGAATCA+TGG | - | Chr6:86551202-86551221 | None:intergenic | 20.0% |
| ! | ATATTTGGACGAGAATAAAA+CGG | + | Chr6:86550744-86550763 | MsG0680034376.01.T01:CDS | 25.0% |
| !!! | AGAAGCTGTTTTGAAATATT+TGG | + | Chr6:86550729-86550748 | MsG0680034376.01.T01:CDS | 25.0% |
| CTAAGAAATTAAGAAAACGG+CGG | - | Chr6:86550387-86550406 | None:intergenic | 30.0% | |
| CTTTAAATGATTGGCAAAAG+AGG | + | Chr6:86551121-86551140 | MsG0680034376.01.T01:CDS | 30.0% | |
| GGTTTGATCCTTTAAATGAT+TGG | + | Chr6:86551112-86551131 | MsG0680034376.01.T01:CDS | 30.0% | |
| ! | ATGATTGGTTGAAGAAATGT+GGG | + | Chr6:86551148-86551167 | MsG0680034376.01.T01:CDS | 30.0% |
| ! | CTCTTTTGCCAATCATTTAA+AGG | - | Chr6:86551123-86551142 | None:intergenic | 30.0% |
| ! | TAAAAAGCATGAATCATGGT+TGG | - | Chr6:86551198-86551217 | None:intergenic | 30.0% |
| ! | TATGATTGGTTGAAGAAATG+TGG | + | Chr6:86551147-86551166 | MsG0680034376.01.T01:CDS | 30.0% |
| !!! | ATACGCTTGTTTTTATTGGT+GGG | + | Chr6:86551091-86551110 | MsG0680034376.01.T01:CDS | 30.0% |
| CCAATAAAAACAAGCGTATC+CGG | - | Chr6:86551090-86551109 | None:intergenic | 35.0% | |
| CGTCATAAGCAAATGAATCA+GGG | - | Chr6:86550609-86550628 | None:intergenic | 35.0% | |
| GTCATAAGCAAATGAATCAG+GGG | - | Chr6:86550608-86550627 | None:intergenic | 35.0% | |
| TCAGTTGATTATGCAAGTCA+AGG | + | Chr6:86551245-86551264 | MsG0680034376.01.T01:CDS | 35.0% | |
| TTAACGCCGAAAATAACGTT+TGG | + | Chr6:86550467-86550486 | MsG0680034376.01.T01:CDS | 35.0% | |
| ! | TAATTTTCCGGCAGAATCTT+CGG | - | Chr6:86550639-86550658 | None:intergenic | 35.0% |
| !! | ACGGAACCAAACGTTATTTT+CGG | - | Chr6:86550476-86550495 | None:intergenic | 35.0% |
| !! | GGCAAAAGAGGTATTATGAT+TGG | + | Chr6:86551133-86551152 | MsG0680034376.01.T01:CDS | 35.0% |
| !! | TTTGGTGTCGATATTATCTC+CGG | - | Chr6:86550530-86550549 | None:intergenic | 35.0% |
| !!! | GATACGCTTGTTTTTATTGG+TGG | + | Chr6:86551090-86551109 | MsG0680034376.01.T01:CDS | 35.0% |
| AAATGAATCAGGGGTGAGAT+AGG | - | Chr6:86550599-86550618 | None:intergenic | 40.0% | |
| AAATTTGGCACATCACGTTG+CGG | + | Chr6:86550825-86550844 | MsG0680034376.01.T01:CDS | 40.0% | |
| ATAATCAACTGACCAGACTC+AGG | - | Chr6:86551237-86551256 | None:intergenic | 40.0% | |
| ATGGGACGGTTAATGTATGT+GGG | + | Chr6:86551031-86551050 | MsG0680034376.01.T01:CDS | 40.0% | |
| ATTGAAATGCGCGATTTCCA+AGG | - | Chr6:86550310-86550329 | None:intergenic | 40.0% | |
| CCATGGAAGAAAATGATCAC+CGG | - | Chr6:86550565-86550584 | None:intergenic | 40.0% | |
| GAAGAAAATGATCACCGGAA+GGG | - | Chr6:86550560-86550579 | None:intergenic | 40.0% | |
| GCGTCATAAGCAAATGAATC+AGG | - | Chr6:86550610-86550629 | None:intergenic | 40.0% | |
| TCTTGACACGTCAGCATATT+CGG | - | Chr6:86550776-86550795 | None:intergenic | 40.0% | |
| TTGAAATGCGCGATTTCCAA+GGG | - | Chr6:86550309-86550328 | None:intergenic | 40.0% | |
| TTTGTCGGGTTTGGATTATC+CGG | + | Chr6:86551068-86551087 | MsG0680034376.01.T01:CDS | 40.0% | |
| ! | ATCATTTTCTTCCATGGAGG+TGG | + | Chr6:86550568-86550587 | MsG0680034376.01.T01:CDS | 40.0% |
| ! | CAACAACGGCGTTAATTTTC+CGG | - | Chr6:86550651-86550670 | None:intergenic | 40.0% |
| ! | GTGATCATTTTCTTCCATGG+AGG | + | Chr6:86550565-86550584 | MsG0680034376.01.T01:CDS | 40.0% |
| !!! | CCGGATACGCTTGTTTTTAT+TGG | + | Chr6:86551087-86551106 | MsG0680034376.01.T01:CDS | 40.0% |
| !!! | GATGTGGAAAGCGTTTTTAC+CGG | + | Chr6:86550990-86551009 | MsG0680034376.01.T01:CDS | 40.0% |
| ATTATCTCCGGCGGAATTGA+CGG | - | Chr6:86550518-86550537 | None:intergenic | 45.0% | |
| CATGGGACGGTTAATGTATG+TGG | + | Chr6:86551030-86551049 | MsG0680034376.01.T01:CDS | 45.0% | |
| CTTGACACGTCAGCATATTC+GGG | - | Chr6:86550775-86550794 | None:intergenic | 45.0% | |
| GATAGGTAAATCCACCTCCA+TGG | - | Chr6:86550582-86550601 | None:intergenic | 45.0% | |
| GGAAGAAAATGATCACCGGA+AGG | - | Chr6:86550561-86550580 | None:intergenic | 45.0% | |
| GTAGCTAACGGAGACAACAA+CGG | - | Chr6:86550665-86550684 | None:intergenic | 45.0% | |
| GTGATGTGCCAAATTTGCAC+CGG | - | Chr6:86550821-86550840 | None:intergenic | 45.0% | |
| GTTAACGATGACGTCTTTGG+TGG | - | Chr6:86550452-86550471 | None:intergenic | 45.0% | |
| TAATGTATGTGGGCCGAATG+CGG | + | Chr6:86551041-86551060 | MsG0680034376.01.T01:CDS | 45.0% | |
| TATCCATCGCAATACGACGA+TGG | + | Chr6:86550706-86550725 | MsG0680034376.01.T01:CDS | 45.0% | |
| ! | AGGAAGGGTTTGGGTTTGTT+TGG | - | Chr6:86550286-86550305 | None:intergenic | 45.0% |
| ! | CCGGTGATCATTTTCTTCCA+TGG | + | Chr6:86550562-86550581 | MsG0680034376.01.T01:CDS | 45.0% |
| ! | TTTCCTGATTTGCCTGAGTC+TGG | + | Chr6:86551222-86551241 | MsG0680034376.01.T01:CDS | 45.0% |
| !!! | GTATCCATCCAGCCGTTTTT+TGG | + | Chr6:86550895-86550914 | MsG0680034376.01.T01:CDS | 45.0% |
| !!! | TGGAAAGCGTTTTTACCGGA+GGG | + | Chr6:86550994-86551013 | MsG0680034376.01.T01:CDS | 45.0% |
| AAATGCGCGATTTCCAAGGG+AGG | - | Chr6:86550306-86550325 | None:intergenic | 50.0% | |
| ACCCGATAACCCGGATTTCT+CGG | - | Chr6:86550873-86550892 | None:intergenic | 50.0% | |
| AGAAATCCGGGTTATCGGGT+TGG | + | Chr6:86550873-86550892 | MsG0680034376.01.T01:CDS | 50.0% | |
| CCTAACGCCATCCCTGTTAA+CGG | + | Chr6:86550421-86550440 | MsG0680034376.01.T01:CDS | 50.0% | |
| CGTTAACAGGGATGGCGTTA+GGG | - | Chr6:86550423-86550442 | None:intergenic | 50.0% | |
| CTCCGAGAAATCCGGGTTAT+CGG | + | Chr6:86550868-86550887 | MsG0680034376.01.T01:CDS | 50.0% | |
| CTCTCCTCCCCACCAAAAAA+CGG | - | Chr6:86550910-86550929 | None:intergenic | 50.0% | |
| CTGTTTGCCGAAGATTCTGC+CGG | + | Chr6:86550629-86550648 | MsG0680034376.01.T01:CDS | 50.0% | |
| GAAAATGATCACCGGAAGGG+TGG | - | Chr6:86550557-86550576 | None:intergenic | 50.0% | |
| GGCGTTAACGATGACGTCTT+TGG | - | Chr6:86550455-86550474 | None:intergenic | 50.0% | |
| GTGTCAAGATGTTTCCTCGC+CGG | + | Chr6:86550787-86550806 | MsG0680034376.01.T01:CDS | 50.0% | |
| GTTAACAGGGATGGCGTTAG+GGG | - | Chr6:86550422-86550441 | None:intergenic | 50.0% | |
| TCCGAGAAATCCGGGTTATC+GGG | + | Chr6:86550869-86550888 | MsG0680034376.01.T01:CDS | 50.0% | |
| TCTCCATCGTCGTATTGCGA+TGG | - | Chr6:86550712-86550731 | None:intergenic | 50.0% | |
| TGACCAGACTCAGGCAAATC+AGG | - | Chr6:86551228-86551247 | None:intergenic | 50.0% | |
| TGCGGTTAGGGTTTGCAAAG+CGG | + | Chr6:86550843-86550862 | MsG0680034376.01.T01:CDS | 50.0% | |
| TGGATACCAACCCGATAACC+CGG | - | Chr6:86550882-86550901 | None:intergenic | 50.0% | |
| TGGTGGAAACGCCGTTAACA+GGG | - | Chr6:86550435-86550454 | None:intergenic | 50.0% | |
| TTCCAAGGGAGGAAGGGTTT+GGG | - | Chr6:86550295-86550314 | None:intergenic | 50.0% | |
| TTGGTGGAAACGCCGTTAAC+AGG | - | Chr6:86550436-86550455 | None:intergenic | 50.0% | |
| TTTCCAAGGGAGGAAGGGTT+TGG | - | Chr6:86550296-86550315 | None:intergenic | 50.0% | |
| !! | AGACAGTGCCGGTGCAAATT+TGG | + | Chr6:86550810-86550829 | MsG0680034376.01.T01:CDS | 50.0% |
| !! | GGTGTCGATATTATCTCCGG+CGG | - | Chr6:86550527-86550546 | None:intergenic | 50.0% |
| !! | TGGTAGTGGGTGTGAAGAGA+CGG | - | Chr6:86550495-86550514 | None:intergenic | 50.0% |
| !!! | CATCCAGCCGTTTTTTGGTG+GGG | + | Chr6:86550900-86550919 | MsG0680034376.01.T01:CDS | 50.0% |
| !!! | CCATCCAGCCGTTTTTTGGT+GGG | + | Chr6:86550899-86550918 | MsG0680034376.01.T01:CDS | 50.0% |
| !!! | GGAAAGCGTTTTTACCGGAG+GGG | + | Chr6:86550995-86551014 | MsG0680034376.01.T01:CDS | 50.0% |
| !!! | GTGGAAAGCGTTTTTACCGG+AGG | + | Chr6:86550993-86551012 | MsG0680034376.01.T01:CDS | 50.0% |
| !!! | TCCATCCAGCCGTTTTTTGG+TGG | + | Chr6:86550898-86550917 | MsG0680034376.01.T01:CDS | 50.0% |
| AACCCAAACCCTTCCTCCCT+TGG | + | Chr6:86550290-86550309 | MsG0680034376.01.T01:CDS | 55.0% | |
| AATGATCACCGGAAGGGTGG+CGG | - | Chr6:86550554-86550573 | None:intergenic | 55.0% | |
| ACTACCACCGTCAATTCCGC+CGG | + | Chr6:86550508-86550527 | MsG0680034376.01.T01:CDS | 55.0% | |
| CCCACCAAAAAACGGCTGGA+TGG | - | Chr6:86550902-86550921 | None:intergenic | 55.0% | |
| CCCGACAAATCCTCCGCATT+CGG | - | Chr6:86551057-86551076 | None:intergenic | 55.0% | |
| CCGAATGCGGAGGATTTGTC+GGG | + | Chr6:86551054-86551073 | MsG0680034376.01.T01:CDS | 55.0% | |
| CCGTTAACAGGGATGGCGTT+AGG | - | Chr6:86550424-86550443 | None:intergenic | 55.0% | |
| CGCGATTTCCAAGGGAGGAA+GGG | - | Chr6:86550301-86550320 | None:intergenic | 55.0% | |
| CGGTTAGGGTTTGCAAAGCG+GGG | + | Chr6:86550845-86550864 | MsG0680034376.01.T01:CDS | 55.0% | |
| GACGCTTCACGTCGCTCTAA+CGG | + | Chr6:86550352-86550371 | MsG0680034376.01.T01:CDS | 55.0% | |
| GCCGAATGCGGAGGATTTGT+CGG | + | Chr6:86551053-86551072 | MsG0680034376.01.T01:CDS | 55.0% | |
| GCGGAATTGACGGTGGTAGT+GGG | - | Chr6:86550508-86550527 | None:intergenic | 55.0% | |
| GCGGTTAGGGTTTGCAAAGC+GGG | + | Chr6:86550844-86550863 | MsG0680034376.01.T01:CDS | 55.0% | |
| GGAAACGCCGTTAACAGGGA+TGG | - | Chr6:86550431-86550450 | None:intergenic | 55.0% | |
| GGCACATCACGTTGCGGTTA+GGG | + | Chr6:86550831-86550850 | MsG0680034376.01.T01:CDS | 55.0% | |
| TGCGATGGATACCGATGCTC+CGG | - | Chr6:86550697-86550716 | None:intergenic | 55.0% | |
| TGCGGAGGATTTGTCGGGTT+TGG | + | Chr6:86551059-86551078 | MsG0680034376.01.T01:CDS | 55.0% | |
| TGGCACATCACGTTGCGGTT+AGG | + | Chr6:86550830-86550849 | MsG0680034376.01.T01:CDS | 55.0% | |
| TGGCGAGGACTGATTGGATG+TGG | + | Chr6:86550974-86550993 | MsG0680034376.01.T01:CDS | 55.0% | |
| TGTCGATGGCGAGGACTGAT+TGG | + | Chr6:86550968-86550987 | MsG0680034376.01.T01:CDS | 55.0% | |
| ! | AGCGTTTTTACCGGAGGGGT+CGG | + | Chr6:86550999-86551018 | MsG0680034376.01.T01:CDS | 55.0% |
| ! | ATTTGCACCGGCACTGTCTC+CGG | - | Chr6:86550809-86550828 | None:intergenic | 55.0% |
| ! | TTTTGGTGGGGAGGAGAGGA+CGG | + | Chr6:86550912-86550931 | MsG0680034376.01.T01:CDS | 55.0% |
| !!! | CGTTTTTTGGTGGGGAGGAG+AGG | + | Chr6:86550908-86550927 | MsG0680034376.01.T01:CDS | 55.0% |
| AAGCGGGGCTCCGAGAAATC+CGG | + | Chr6:86550860-86550879 | MsG0680034376.01.T01:CDS | 60.0% | |
| AGAGGACGGAGGCGGAGATT+AGG | + | Chr6:86550926-86550945 | MsG0680034376.01.T01:CDS | 60.0% | |
| AGGGGTCGGATCGCGATCAT+GGG | + | Chr6:86551013-86551032 | MsG0680034376.01.T01:CDS | 60.0% | |
| ATCTCCGGCGGAATTGACGG+TGG | - | Chr6:86550515-86550534 | None:intergenic | 60.0% | |
| CACCAAAACCGCCACCCTTC+CGG | + | Chr6:86550543-86550562 | MsG0680034376.01.T01:CDS | 60.0% | |
| CCTCCCCACCAAAAAACGGC+TGG | - | Chr6:86550906-86550925 | None:intergenic | 60.0% | |
| CGGCGTGTGACGGTAGCTAA+CGG | - | Chr6:86550677-86550696 | None:intergenic | 60.0% | |
| CGTTAGCTACCGTCACACGC+CGG | + | Chr6:86550675-86550694 | MsG0680034376.01.T01:CDS | 60.0% | |
| GAGGCGGAGATTAGGCTCGA+GGG | + | Chr6:86550934-86550953 | MsG0680034376.01.T01:CDS | 60.0% | |
| GCGCGATTTCCAAGGGAGGA+AGG | - | Chr6:86550302-86550321 | None:intergenic | 60.0% | |
| GGCGGAATTGACGGTGGTAG+TGG | - | Chr6:86550509-86550528 | None:intergenic | 60.0% | |
| GTCCTCGCCATCGACACCAA+CGG | - | Chr6:86550964-86550983 | None:intergenic | 60.0% | |
| GTCGGATCGCGATCATGGGA+CGG | + | Chr6:86551017-86551036 | MsG0680034376.01.T01:CDS | 60.0% | |
| TAGGCTCGAGGGATCACCGT+TGG | + | Chr6:86550945-86550964 | MsG0680034376.01.T01:CDS | 60.0% | |
| TGTATGTGGGCCGAATGCGG+AGG | + | Chr6:86551044-86551063 | MsG0680034376.01.T01:CDS | 60.0% | |
| !! | GGGATCACCGTTGGTGTCGA+TGG | + | Chr6:86550954-86550973 | MsG0680034376.01.T01:CDS | 60.0% |
| !!! | CCAGCCGTTTTTTGGTGGGG+AGG | + | Chr6:86550903-86550922 | MsG0680034376.01.T01:CDS | 60.0% |
| ACCGATGCTCCGGCGTGTGA+CGG | - | Chr6:86550687-86550706 | None:intergenic | 65.0% | |
| ACCGTCACACGCCGGAGCAT+CGG | + | Chr6:86550683-86550702 | MsG0680034376.01.T01:CDS | 65.0% | |
| AGCGGGGCTCCGAGAAATCC+GGG | + | Chr6:86550861-86550880 | MsG0680034376.01.T01:CDS | 65.0% | |
| GAGGGGTCGGATCGCGATCA+TGG | + | Chr6:86551012-86551031 | MsG0680034376.01.T01:CDS | 65.0% | |
| GGAGGCGGAGATTAGGCTCG+AGG | + | Chr6:86550933-86550952 | MsG0680034376.01.T01:CDS | 65.0% | |
| TGATCGCGATCCGACCCCTC+CGG | - | Chr6:86551012-86551031 | None:intergenic | 65.0% | |
| TTCCTCGCCGGAGACAGTGC+CGG | + | Chr6:86550799-86550818 | MsG0680034376.01.T01:CDS | 65.0% | |
| !! | CACCGGAAGGGTGGCGGTTT+TGG | - | Chr6:86550548-86550567 | None:intergenic | 65.0% |
| !! | CACCGTTGGTGTCGATGGCG+AGG | + | Chr6:86550959-86550978 | MsG0680034376.01.T01:CDS | 65.0% |
| TGGGGAGGAGAGGACGGAGG+CGG | + | Chr6:86550918-86550937 | MsG0680034376.01.T01:CDS | 70.0% | |
| TGGTGGGGAGGAGAGGACGG+AGG | + | Chr6:86550915-86550934 | MsG0680034376.01.T01:CDS | 70.0% | |
| CACCGGCACTGTCTCCGGCG+AGG | - | Chr6:86550804-86550823 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 86550274 | 86551302 | 86550274 | ID=MsG0680034376.01;Name=MsG0680034376.01 |
| Chr6 | mRNA | 86550274 | 86551302 | 86550274 | ID=MsG0680034376.01.T01;Parent=MsG0680034376.01;Name=MsG0680034376.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|342 |
| Chr6 | exon | 86550274 | 86551302 | 86550274 | ID=MsG0680034376.01.T01:exon:18188;Parent=MsG0680034376.01.T01 |
| Chr6 | CDS | 86550274 | 86551302 | 86550274 | ID=MsG0680034376.01.T01:cds;Parent=MsG0680034376.01.T01 |
| Gene Sequence |
| Protein sequence |
