Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034763.01.T01 | XP_013452777.1 | 98.477 | 197 | 3 | 0 | 1 | 197 | 1 | 197 | 7.80E-139 | 395 |
| MsG0680034763.01.T04 | XP_013452777.1 | 100 | 53 | 0 | 0 | 1 | 53 | 145 | 197 | 1.41E-29 | 112 |
| MsG0680034763.01.T03 | XP_013452777.1 | 98.601 | 143 | 2 | 0 | 1 | 143 | 55 | 197 | 4.16E-99 | 293 |
| MsG0680034763.01.T02 | XP_013452777.1 | 98.352 | 182 | 3 | 0 | 1 | 182 | 16 | 197 | 5.25E-127 | 365 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034763.01.T01 | Q93W54 | 67.005 | 197 | 65 | 0 | 1 | 197 | 1 | 197 | 2.07E-100 | 291 |
| MsG0680034763.01.T02 | Q93W54 | 67.033 | 182 | 60 | 0 | 1 | 182 | 16 | 197 | 1.36E-92 | 271 |
| MsG0680034763.01.T03 | Q93W54 | 65.035 | 143 | 50 | 0 | 1 | 143 | 55 | 197 | 3.18E-71 | 215 |
| MsG0680034763.01.T04 | Q93W54 | 75.472 | 53 | 13 | 0 | 1 | 53 | 145 | 197 | 3.18E-24 | 91.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034763.01.T01 | A0A072ULV3 | 98.477 | 197 | 3 | 0 | 1 | 197 | 1 | 197 | 3.73e-139 | 395 |
| MsG0680034763.01.T02 | A0A072ULV3 | 98.352 | 182 | 3 | 0 | 1 | 182 | 16 | 197 | 2.51e-127 | 365 |
| MsG0680034763.01.T03 | A0A072ULV3 | 98.601 | 143 | 2 | 0 | 1 | 143 | 55 | 197 | 1.99e-99 | 293 |
| MsG0680034763.01.T04 | A0A072ULV3 | 100.000 | 53 | 0 | 0 | 1 | 53 | 145 | 197 | 6.75e-30 | 112 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024119.01 | MsG0680034763.01 | 0.821051 | 4.957702e-53 | 3.990630e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034763.01.T01 | MTR_6g075160 | 98.477 | 197 | 3 | 0 | 1 | 197 | 1 | 197 | 9.45e-143 | 395 |
| MsG0680034763.01.T04 | MTR_6g075160 | 100.000 | 53 | 0 | 0 | 1 | 53 | 145 | 197 | 1.71e-33 | 112 |
| MsG0680034763.01.T03 | MTR_6g075160 | 98.601 | 143 | 2 | 0 | 1 | 143 | 55 | 197 | 5.03e-103 | 293 |
| MsG0680034763.01.T03 | MTR_0681s0020 | 33.636 | 110 | 70 | 2 | 27 | 135 | 1325 | 1432 | 7.70e-11 | 59.3 |
| MsG0680034763.01.T02 | MTR_6g075160 | 98.352 | 182 | 3 | 0 | 1 | 182 | 16 | 197 | 6.36e-131 | 365 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034763.01.T01 | AT5G08335 | 67.005 | 197 | 65 | 0 | 1 | 197 | 1 | 197 | 2.11e-101 | 291 |
| MsG0680034763.01.T01 | AT5G08335 | 67.005 | 197 | 65 | 0 | 1 | 197 | 1 | 197 | 2.11e-101 | 291 |
| MsG0680034763.01.T01 | AT5G23320 | 67.005 | 197 | 65 | 0 | 1 | 197 | 1 | 197 | 1.96e-97 | 281 |
| MsG0680034763.01.T01 | AT5G23320 | 66.327 | 196 | 66 | 0 | 2 | 197 | 27 | 222 | 1.16e-95 | 277 |
| MsG0680034763.01.T04 | AT5G08335 | 75.472 | 53 | 13 | 0 | 1 | 53 | 145 | 197 | 3.24e-25 | 91.7 |
| MsG0680034763.01.T04 | AT5G08335 | 75.472 | 53 | 13 | 0 | 1 | 53 | 145 | 197 | 3.24e-25 | 91.7 |
| MsG0680034763.01.T04 | AT5G23320 | 73.585 | 53 | 14 | 0 | 1 | 53 | 145 | 197 | 7.90e-20 | 77.8 |
| MsG0680034763.01.T04 | AT5G23320 | 73.585 | 53 | 14 | 0 | 1 | 53 | 170 | 222 | 1.40e-19 | 77.8 |
| MsG0680034763.01.T03 | AT5G08335 | 65.035 | 143 | 50 | 0 | 1 | 143 | 55 | 197 | 3.24e-72 | 215 |
| MsG0680034763.01.T03 | AT5G08335 | 65.035 | 143 | 50 | 0 | 1 | 143 | 55 | 197 | 3.24e-72 | 215 |
| MsG0680034763.01.T03 | AT5G23320 | 66.434 | 143 | 48 | 0 | 1 | 143 | 55 | 197 | 6.06e-70 | 209 |
| MsG0680034763.01.T03 | AT5G23320 | 66.434 | 143 | 48 | 0 | 1 | 143 | 80 | 222 | 1.77e-69 | 209 |
| MsG0680034763.01.T02 | AT5G08335 | 67.033 | 182 | 60 | 0 | 1 | 182 | 16 | 197 | 1.38e-93 | 271 |
| MsG0680034763.01.T02 | AT5G08335 | 67.033 | 182 | 60 | 0 | 1 | 182 | 16 | 197 | 1.38e-93 | 271 |
| MsG0680034763.01.T02 | AT5G23320 | 66.484 | 182 | 61 | 0 | 1 | 182 | 16 | 197 | 8.47e-90 | 261 |
| MsG0680034763.01.T02 | AT5G23320 | 66.484 | 182 | 61 | 0 | 1 | 182 | 41 | 222 | 2.34e-89 | 261 |
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGGGTGATCAGTGATTT+AGG | 0.140848 | 6:+94171534 | MsG0680034763.01.T01:CDS |
| AATCGTTTACTCATATTATA+AGG | 0.176162 | 6:+94171616 | MsG0680034763.01.T01:CDS |
| TGCAATCCTTTATCAACTAT+TGG | 0.236590 | 6:+94171750 | MsG0680034763.01.T01:CDS |
| GGTATTGTGGTTTCTTCATT+TGG | 0.292016 | 6:+94171703 | MsG0680034763.01.T01:CDS |
| AGTATTCCTCATAAGGAATT+CGG | 0.300337 | 6:-94171806 | None:intergenic |
| TTGTGGTAGGTGAAATTATA+AGG | 0.312232 | 6:+94171568 | MsG0680034763.01.T01:CDS |
| TCCTTAGCTACACTGCTTAC+AGG | 0.316459 | 6:+94171322 | MsG0680034763.01.T01:CDS |
| GATTAGCAAACACTATCTTT+TGG | 0.337388 | 6:+94171449 | MsG0680034763.01.T01:CDS |
| GCAAATCCAATAGTTGATAA+AGG | 0.380078 | 6:-94171756 | None:intergenic |
| TTGGATTTGCAGTAGTTGTT+TGG | 0.383742 | 6:+94171769 | MsG0680034763.01.T01:CDS |
| TTTCTAGCCGCCGTCATTCA+TGG | 0.387546 | 6:+94171396 | MsG0680034763.01.T01:CDS |
| ATTGGGTGATCAGTGATTTA+GGG | 0.396206 | 6:+94171535 | MsG0680034763.01.T01:CDS |
| CAGTGATTTAGGGTTGGTAT+TGG | 0.399417 | 6:+94171545 | MsG0680034763.01.T01:CDS |
| TATGCTCAGCAAGTTGTGTC+TGG | 0.403992 | 6:+94171864 | MsG0680034763.01.T01:CDS |
| TGCATTCTTTCCTGAGCTAA+AGG | 0.408975 | 6:+94171509 | MsG0680034763.01.T01:CDS |
| GATAGTGTTTGCTAATCAAA+AGG | 0.415650 | 6:-94171443 | None:intergenic |
| TTCTACCCGAATTCCTTATG+AGG | 0.417461 | 6:+94171800 | MsG0680034763.01.T01:CDS |
| ATCGTTTACTCATATTATAA+GGG | 0.434247 | 6:+94171617 | MsG0680034763.01.T01:CDS |
| TCCTGAGCTAAAGGAACATT+GGG | 0.439666 | 6:+94171518 | MsG0680034763.01.T01:CDS |
| TTTAGGGTTGGTATTGGTTG+TGG | 0.442478 | 6:+94171551 | MsG0680034763.01.T01:CDS |
| GTATTCCTCATAAGGAATTC+GGG | 0.443632 | 6:-94171805 | None:intergenic |
| TATAAGTTTATTCGCCATCC+AGG | 0.449544 | 6:+94171681 | MsG0680034763.01.T01:CDS |
| ATCAGATGGTGATGTTCATC+AGG | 0.461241 | 6:-94171645 | None:intergenic |
| ACCCAATGTTCCTTTAGCTC+AGG | 0.472967 | 6:-94171519 | None:intergenic |
| TTCCTGAGCTAAAGGAACAT+TGG | 0.485094 | 6:+94171517 | MsG0680034763.01.T01:CDS |
| GCCTGTAAGCAGTGTAGCTA+AGG | 0.495217 | 6:-94171323 | None:intergenic |
| TTCTAGCCGCCGTCATTCAT+GGG | 0.498626 | 6:+94171397 | MsG0680034763.01.T01:CDS |
| ATCGGTCTTCAGTTAACAAA+TGG | 0.499243 | 6:-94171891 | None:intergenic |
| ATTGCTAGAATATTTAATTG+AGG | 0.505523 | 6:+94171485 | MsG0680034763.01.T01:CDS |
| ATAGTGTTTGCTAATCAAAA+GGG | 0.520804 | 6:-94171442 | None:intergenic |
| GGGTTGGTATTGGTTGTGGT+AGG | 0.533032 | 6:+94171555 | MsG0680034763.01.T01:CDS |
| AAGGTGGCGATAATAACAGC+AGG | 0.537746 | 6:+94171591 | MsG0680034763.01.T01:CDS |
| GGTGATCAGTGATTTAGGGT+TGG | 0.538136 | 6:+94171539 | MsG0680034763.01.T01:CDS |
| GGTAGGTGAAATTATAAGGA+AGG | 0.546342 | 6:+94171572 | MsG0680034763.01.T01:CDS |
| ATTCGCCATCCAGGGTATTG+TGG | 0.547036 | 6:+94171690 | MsG0680034763.01.T01:CDS |
| ATGCTCAGCAAGTTGTGTCT+GGG | 0.548103 | 6:+94171865 | MsG0680034763.01.T01:CDS |
| ACTTGATTTCCCATGAATGA+CGG | 0.558189 | 6:-94171406 | None:intergenic |
| ATATACCATGCGTAATCAGA+TGG | 0.584498 | 6:-94171659 | None:intergenic |
| ATAAGTTTATTCGCCATCCA+GGG | 0.606385 | 6:+94171682 | MsG0680034763.01.T01:CDS |
| AGGTGAAATTATAAGGAAGG+TGG | 0.612618 | 6:+94171575 | MsG0680034763.01.T01:CDS |
| CATCACCATCTGATTACGCA+TGG | 0.617251 | 6:+94171654 | MsG0680034763.01.T01:CDS |
| AGAAACCACAATACCCTGGA+TGG | 0.622987 | 6:-94171695 | None:intergenic |
| ATGAAGAAACCACAATACCC+TGG | 0.625175 | 6:-94171699 | None:intergenic |
| GATGGTGATGTTCATCAGGA+CGG | 0.625500 | 6:-94171641 | None:intergenic |
| TGCTCAGCAAGTTGTGTCTG+GGG | 0.663091 | 6:+94171866 | MsG0680034763.01.T01:CDS |
| TGATTTCCCATGAATGACGG+CGG | 0.666534 | 6:-94171403 | None:intergenic |
| AGGTGGCGATAATAACAGCA+GGG | 0.708107 | 6:+94171592 | MsG0680034763.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCGTTTACTCATATTATA+AGG | + | Chr6:94171616-94171635 | MsG0680034763.01.T01:CDS | 20.0% |
| !! | ATCGTTTACTCATATTATAA+GGG | + | Chr6:94171617-94171636 | MsG0680034763.01.T01:CDS | 20.0% |
| !! | ATTGCTAGAATATTTAATTG+AGG | + | Chr6:94171485-94171504 | MsG0680034763.01.T01:CDS | 20.0% |
| !!! | TACTTTTTAAGACAGTTTTT+TGG | + | Chr6:94171825-94171844 | MsG0680034763.01.T01:CDS | 20.0% |
| !! | ATAGTGTTTGCTAATCAAAA+GGG | - | Chr6:94171445-94171464 | None:intergenic | 25.0% |
| !!! | CTTAAAAAGTATTCCTCATA+AGG | - | Chr6:94171816-94171835 | None:intergenic | 25.0% |
| AGTATTCCTCATAAGGAATT+CGG | - | Chr6:94171809-94171828 | None:intergenic | 30.0% | |
| TGCAATCCTTTATCAACTAT+TGG | + | Chr6:94171750-94171769 | MsG0680034763.01.T01:CDS | 30.0% | |
| TTGTGGTAGGTGAAATTATA+AGG | + | Chr6:94171568-94171587 | MsG0680034763.01.T01:CDS | 30.0% | |
| ! | GATAGTGTTTGCTAATCAAA+AGG | - | Chr6:94171446-94171465 | None:intergenic | 30.0% |
| ! | GATTAGCAAACACTATCTTT+TGG | + | Chr6:94171449-94171468 | MsG0680034763.01.T01:CDS | 30.0% |
| !! | GCAAATCCAATAGTTGATAA+AGG | - | Chr6:94171759-94171778 | None:intergenic | 30.0% |
| ACTTGATTTCCCATGAATGA+CGG | - | Chr6:94171409-94171428 | None:intergenic | 35.0% | |
| AGGTGAAATTATAAGGAAGG+TGG | + | Chr6:94171575-94171594 | MsG0680034763.01.T01:CDS | 35.0% | |
| ATAAGTTTATTCGCCATCCA+GGG | + | Chr6:94171682-94171701 | MsG0680034763.01.T01:CDS | 35.0% | |
| ATATACCATGCGTAATCAGA+TGG | - | Chr6:94171662-94171681 | None:intergenic | 35.0% | |
| ATTGGGTGATCAGTGATTTA+GGG | + | Chr6:94171535-94171554 | MsG0680034763.01.T01:CDS | 35.0% | |
| GGTAGGTGAAATTATAAGGA+AGG | + | Chr6:94171572-94171591 | MsG0680034763.01.T01:CDS | 35.0% | |
| GGTATTGTGGTTTCTTCATT+TGG | + | Chr6:94171703-94171722 | MsG0680034763.01.T01:CDS | 35.0% | |
| GTATTCCTCATAAGGAATTC+GGG | - | Chr6:94171808-94171827 | None:intergenic | 35.0% | |
| TATAAGTTTATTCGCCATCC+AGG | + | Chr6:94171681-94171700 | MsG0680034763.01.T01:CDS | 35.0% | |
| TTGGATTTGCAGTAGTTGTT+TGG | + | Chr6:94171769-94171788 | MsG0680034763.01.T01:CDS | 35.0% | |
| ! | CAAACACTATCTTTTGGCAA+TGG | + | Chr6:94171455-94171474 | MsG0680034763.01.T01:CDS | 35.0% |
| ATGAAGAAACCACAATACCC+TGG | - | Chr6:94171702-94171721 | None:intergenic | 40.0% | |
| CATTGGGTGATCAGTGATTT+AGG | + | Chr6:94171534-94171553 | MsG0680034763.01.T01:CDS | 40.0% | |
| TCCTGAGCTAAAGGAACATT+GGG | + | Chr6:94171518-94171537 | MsG0680034763.01.T01:CDS | 40.0% | |
| TGCATTCTTTCCTGAGCTAA+AGG | + | Chr6:94171509-94171528 | MsG0680034763.01.T01:CDS | 40.0% | |
| TTCCTGAGCTAAAGGAACAT+TGG | + | Chr6:94171517-94171536 | MsG0680034763.01.T01:CDS | 40.0% | |
| TTCTACCCGAATTCCTTATG+AGG | + | Chr6:94171800-94171819 | MsG0680034763.01.T01:CDS | 40.0% | |
| ! | ATCAGATGGTGATGTTCATC+AGG | - | Chr6:94171648-94171667 | None:intergenic | 40.0% |
| !! | CAGTGATTTAGGGTTGGTAT+TGG | + | Chr6:94171545-94171564 | MsG0680034763.01.T01:CDS | 40.0% |
| !! | TTTAGGGTTGGTATTGGTTG+TGG | + | Chr6:94171551-94171570 | MsG0680034763.01.T01:CDS | 40.0% |
| AAGGTGGCGATAATAACAGC+AGG | + | Chr6:94171591-94171610 | MsG0680034763.01.T01:CDS | 45.0% | |
| AGAAACCACAATACCCTGGA+TGG | - | Chr6:94171698-94171717 | None:intergenic | 45.0% | |
| AGGTGGCGATAATAACAGCA+GGG | + | Chr6:94171592-94171611 | MsG0680034763.01.T01:CDS | 45.0% | |
| ATGCTCAGCAAGTTGTGTCT+GGG | + | Chr6:94171865-94171884 | MsG0680034763.01.T01:CDS | 45.0% | |
| CATCACCATCTGATTACGCA+TGG | + | Chr6:94171654-94171673 | MsG0680034763.01.T01:CDS | 45.0% | |
| TATGCTCAGCAAGTTGTGTC+TGG | + | Chr6:94171864-94171883 | MsG0680034763.01.T01:CDS | 45.0% | |
| TCCTTAGCTACACTGCTTAC+AGG | + | Chr6:94171322-94171341 | MsG0680034763.01.T01:CDS | 45.0% | |
| TGATTTCCCATGAATGACGG+CGG | - | Chr6:94171406-94171425 | None:intergenic | 45.0% | |
| ! | ACCCAATGTTCCTTTAGCTC+AGG | - | Chr6:94171522-94171541 | None:intergenic | 45.0% |
| ! | GATGGTGATGTTCATCAGGA+CGG | - | Chr6:94171644-94171663 | None:intergenic | 45.0% |
| ! | GGTGATCAGTGATTTAGGGT+TGG | + | Chr6:94171539-94171558 | MsG0680034763.01.T01:CDS | 45.0% |
| GCCTGTAAGCAGTGTAGCTA+AGG | - | Chr6:94171326-94171345 | None:intergenic | 50.0% | |
| TGCTCAGCAAGTTGTGTCTG+GGG | + | Chr6:94171866-94171885 | MsG0680034763.01.T01:CDS | 50.0% | |
| TTCTAGCCGCCGTCATTCAT+GGG | + | Chr6:94171397-94171416 | MsG0680034763.01.T01:CDS | 50.0% | |
| ! | TTTCTAGCCGCCGTCATTCA+TGG | + | Chr6:94171396-94171415 | MsG0680034763.01.T01:CDS | 50.0% |
| !! | ATTCGCCATCCAGGGTATTG+TGG | + | Chr6:94171690-94171709 | MsG0680034763.01.T01:CDS | 50.0% |
| !! | GGGTTGGTATTGGTTGTGGT+AGG | + | Chr6:94171555-94171574 | MsG0680034763.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 94171312 | 94171905 | 94171312 | ID=MsG0680034763.01;Name=MsG0680034763.01 |
| Chr6 | mRNA | 94171312 | 94171905 | 94171312 | ID=MsG0680034763.01.T01;Parent=MsG0680034763.01;Name=MsG0680034763.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|197 |
| Chr6 | exon | 94171312 | 94171905 | 94171312 | ID=MsG0680034763.01.T01:exon:14574;Parent=MsG0680034763.01.T01 |
| Chr6 | CDS | 94171312 | 94171905 | 94171312 | ID=MsG0680034763.01.T01:cds;Parent=MsG0680034763.01.T01 |
| Chr6 | mRNA | 94171357 | 94171905 | 94171357 | ID=MsG0680034763.01.T02;Parent=MsG0680034763.01;Name=MsG0680034763.01.T02;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|182 |
| Chr6 | exon | 94171357 | 94171905 | 94171357 | ID=MsG0680034763.01.T02:exon:14575;Parent=MsG0680034763.01.T02 |
| Chr6 | CDS | 94171357 | 94171905 | 94171357 | ID=MsG0680034763.01.T02:cds;Parent=MsG0680034763.01.T02 |
| Chr6 | mRNA | 94171474 | 94171905 | 94171474 | ID=MsG0680034763.01.T03;Parent=MsG0680034763.01;Name=MsG0680034763.01.T03;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|143 |
| Chr6 | exon | 94171474 | 94171905 | 94171474 | ID=MsG0680034763.01.T03:exon:14576;Parent=MsG0680034763.01.T03 |
| Chr6 | CDS | 94171474 | 94171905 | 94171474 | ID=MsG0680034763.01.T03:cds;Parent=MsG0680034763.01.T03 |
| Chr6 | mRNA | 94171744 | 94171905 | 94171744 | ID=MsG0680034763.01.T04;Parent=MsG0680034763.01;Name=MsG0680034763.01.T04;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|53 |
| Chr6 | exon | 94171744 | 94171905 | 94171744 | ID=MsG0680034763.01.T04:exon:14577;Parent=MsG0680034763.01.T04 |
| Chr6 | CDS | 94171744 | 94171905 | 94171744 | ID=MsG0680034763.01.T04:cds;Parent=MsG0680034763.01.T04 |
| Gene Sequence |
| Protein sequence |
