Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035762.01.T01 | XP_013470383.1 | 94.828 | 116 | 6 | 0 | 1 | 116 | 104 | 219 | 1.55E-74 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035762.01.T01 | Q9FMW4 | 67.544 | 114 | 37 | 0 | 2 | 115 | 100 | 213 | 8.38E-52 | 165 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035762.01.T01 | B7FJD2 | 94.828 | 116 | 6 | 0 | 1 | 116 | 104 | 219 | 7.40e-75 | 230 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032165.01 | MsG0680035762.01 | 0.805042 | 1.615448e-49 | 8.460060e-47 |
| MsG0680035762.01 | MsG0680035763.01 | 0.887774 | 1.062166e-72 | 8.009071e-69 |
| MsG0680035762.01 | MsG0880046989.01 | 0.807265 | 5.499200e-50 | 3.049294e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035762.01.T02 | MTR_1g114640 | 89.655 | 29 | 3 | 0 | 1 | 29 | 191 | 219 | 9.59e-15 | 63.9 |
| MsG0680035762.01.T02 | MTR_1g114680 | 85.714 | 28 | 4 | 0 | 1 | 28 | 133 | 160 | 4.90e-13 | 58.5 |
| MsG0680035762.01.T01 | MTR_1g114640 | 94.828 | 116 | 6 | 0 | 1 | 116 | 104 | 219 | 1.88e-78 | 230 |
| MsG0680035762.01.T01 | MTR_1g114680 | 83.333 | 108 | 18 | 0 | 8 | 115 | 53 | 160 | 6.02e-56 | 172 |
| MsG0680035762.01.T01 | MTR_1g033750 | 68.696 | 115 | 36 | 0 | 1 | 115 | 106 | 220 | 1.35e-55 | 172 |
| MsG0680035762.01.T01 | MTR_1g114690 | 64.348 | 115 | 41 | 0 | 1 | 115 | 101 | 215 | 1.55e-51 | 162 |
| MsG0680035762.01.T01 | MTR_1g115130 | 64.035 | 114 | 41 | 0 | 2 | 115 | 78 | 191 | 1.38e-50 | 159 |
| MsG0680035762.01.T01 | MTR_1g114750 | 60.526 | 114 | 45 | 0 | 2 | 115 | 105 | 218 | 1.77e-49 | 157 |
| MsG0680035762.01.T01 | MTR_1g114690 | 61.739 | 115 | 44 | 0 | 1 | 115 | 101 | 215 | 3.75e-49 | 156 |
| MsG0680035762.01.T01 | MTR_1g114690 | 50.336 | 149 | 40 | 1 | 1 | 115 | 101 | 249 | 1.05e-44 | 145 |
| MsG0680035762.01.T01 | MTR_6g077990 | 60.177 | 113 | 45 | 0 | 3 | 115 | 41 | 153 | 1.82e-43 | 139 |
| MsG0680035762.01.T01 | MTR_8g100065 | 60.526 | 114 | 44 | 1 | 3 | 115 | 69 | 182 | 3.11e-43 | 140 |
| MsG0680035762.01.T01 | MTR_8g100065 | 60.526 | 114 | 44 | 1 | 3 | 115 | 91 | 204 | 8.81e-43 | 139 |
| MsG0680035762.01.T01 | MTR_6g077990 | 60.177 | 113 | 45 | 0 | 3 | 115 | 83 | 195 | 1.22e-42 | 139 |
| MsG0680035762.01.T01 | MTR_6g077990 | 60.177 | 113 | 45 | 0 | 3 | 115 | 84 | 196 | 1.31e-42 | 139 |
| MsG0680035762.01.T01 | MTR_1g114640 | 96.774 | 62 | 2 | 0 | 1 | 62 | 104 | 165 | 2.25e-36 | 122 |
| MsG0680035762.01.T01 | MTR_7g083570 | 44.737 | 114 | 63 | 0 | 2 | 115 | 161 | 274 | 2.91e-31 | 112 |
| MsG0680035762.01.T01 | MTR_8g069110 | 41.379 | 116 | 65 | 2 | 1 | 115 | 154 | 267 | 4.15e-25 | 95.9 |
| MsG0680035762.01.T01 | MTR_3g100750 | 37.391 | 115 | 69 | 2 | 2 | 115 | 143 | 255 | 2.86e-24 | 93.6 |
| MsG0680035762.01.T01 | MTR_1g114690 | 68.254 | 63 | 20 | 0 | 1 | 63 | 101 | 163 | 3.26e-24 | 92.0 |
| MsG0680035762.01.T01 | MTR_6g077990 | 60.938 | 64 | 25 | 0 | 3 | 66 | 83 | 146 | 3.62e-20 | 80.5 |
| MsG0680035762.01.T01 | MTR_8g100065 | 63.934 | 61 | 21 | 1 | 3 | 62 | 69 | 129 | 5.02e-20 | 79.3 |
| MsG0680035762.01.T01 | MTR_2g076350 | 39.130 | 115 | 69 | 1 | 2 | 115 | 111 | 225 | 5.58e-20 | 81.6 |
| MsG0680035762.01.T01 | MTR_8g100065 | 62.903 | 62 | 22 | 1 | 2 | 62 | 90 | 151 | 8.76e-20 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035762.01.T01 | AT5G23370 | 67.544 | 114 | 37 | 0 | 2 | 115 | 100 | 213 | 8.54e-53 | 165 |
| MsG0680035762.01.T01 | AT5G08350 | 67.257 | 113 | 37 | 0 | 3 | 115 | 100 | 212 | 2.69e-52 | 164 |
| MsG0680035762.01.T01 | AT5G23350 | 66.667 | 114 | 38 | 0 | 2 | 115 | 100 | 213 | 5.64e-51 | 160 |
| MsG0680035762.01.T01 | AT5G23360 | 63.717 | 113 | 41 | 0 | 3 | 115 | 93 | 205 | 8.35e-49 | 155 |
| MsG0680035762.01.T01 | AT4G01600 | 47.368 | 114 | 59 | 1 | 4 | 116 | 73 | 186 | 3.89e-31 | 109 |
| MsG0680035762.01.T01 | AT4G01600 | 47.368 | 114 | 59 | 1 | 4 | 116 | 107 | 220 | 1.75e-30 | 108 |
| MsG0680035762.01.T01 | AT4G01600 | 47.368 | 114 | 59 | 1 | 4 | 116 | 112 | 225 | 2.38e-30 | 108 |
| MsG0680035762.01.T01 | AT5G13200 | 41.818 | 110 | 64 | 0 | 2 | 111 | 147 | 256 | 7.01e-30 | 108 |
| MsG0680035762.01.T01 | AT1G28200 | 40.000 | 115 | 66 | 2 | 2 | 115 | 142 | 254 | 7.68e-25 | 95.1 |
| MsG0680035762.01.T01 | AT1G28200 | 40.000 | 115 | 66 | 2 | 2 | 115 | 142 | 254 | 7.68e-25 | 95.1 |
| MsG0680035762.01.T01 | AT2G22475 | 39.130 | 115 | 67 | 2 | 2 | 115 | 179 | 291 | 6.28e-24 | 93.2 |
| MsG0680035762.01.T01 | AT5G13200 | 40.323 | 62 | 37 | 0 | 2 | 63 | 147 | 208 | 2.89e-12 | 60.8 |
Find 15 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTTCGACTTCTGGTTTA+TGG | 0.139805 | 6:-111908490 | MsG0680035762.01.T01:CDS |
| CTTTGGATGGTTTCGACTTC+TGG | 0.157924 | 6:-111908497 | MsG0680035762.01.T01:CDS |
| GGTTTCGACTTCTGGTTTAT+GGG | 0.291409 | 6:-111908489 | MsG0680035762.01.T01:CDS |
| ACTCTACTCAATTCTCCTTT+TGG | 0.316608 | 6:+111908729 | None:intergenic |
| TTATGGGTTTCTTTAATTAT+CGG | 0.380305 | 6:-111908473 | MsG0680035762.01.T01:CDS |
| ATGAAAAGCAGGCCAGCAAT+AGG | 0.433249 | 6:+111908804 | None:intergenic |
| TTCAGAAAAGTACATAGAAA+TGG | 0.441449 | 6:-111908523 | MsG0680035762.01.T01:CDS |
| ACAACATCAGGTCCTATTGC+TGG | 0.447702 | 6:-111908816 | MsG0680035762.01.T01:CDS |
| ATCAAGATCTCTTCACCAAA+AGG | 0.460333 | 6:-111908744 | MsG0680035762.01.T01:CDS |
| GTACATAGAAATGGTCACTT+TGG | 0.477400 | 6:-111908514 | MsG0680035762.01.T01:CDS |
| AGCTTCAAGATATCTCCAAC+AGG | 0.488616 | 6:-111908448 | MsG0680035762.01.T01:CDS |
| TGTTACTTATCAACAACATC+AGG | 0.514352 | 6:-111908828 | MsG0680035762.01.T01:CDS |
| ATAGAAATGGTCACTTTGGA+TGG | 0.523720 | 6:-111908510 | MsG0680035762.01.T01:CDS |
| TGTGAGTGGAAATGAAAAGC+AGG | 0.561458 | 6:+111908793 | None:intergenic |
| ACAAAGAGCAACTTTGTGAG+TGG | 0.605272 | 6:+111908779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTCTTATAACTATTTT+AGG | - | Chr6:111908712-111908731 | MsG0680035762.01.T01:intron | 10.0% |
| !! | TTATGGGTTTCTTTAATTAT+CGG | - | Chr6:111908843-111908862 | MsG0680035762.01.T01:CDS | 20.0% |
| !!! | ACTTACTTTGTTTACTATTT+CGG | - | Chr6:111908668-111908687 | MsG0680035762.01.T01:intron | 20.0% |
| ! | AATAGTAAACAAAGTAAGTG+TGG | + | Chr6:111908667-111908686 | None:intergenic | 25.0% |
| ! | GCAAAAAAATCATCTTATAG+CGG | + | Chr6:111908613-111908632 | None:intergenic | 25.0% |
| !! | TTCAGAAAAGTACATAGAAA+TGG | - | Chr6:111908793-111908812 | MsG0680035762.01.T01:CDS | 25.0% |
| ! | TGTTACTTATCAACAACATC+AGG | - | Chr6:111908488-111908507 | MsG0680035762.01.T01:CDS | 30.0% |
| !! | GAACTTTTTAGCGTTAAAGA+AGG | - | Chr6:111908440-111908459 | MsG0680035762.01.T01:CDS | 30.0% |
| !!! | CTTTCTTCACATTTTGACTT+TGG | + | Chr6:111908772-111908791 | None:intergenic | 30.0% |
| ATCAAGATCTCTTCACCAAA+AGG | - | Chr6:111908572-111908591 | MsG0680035762.01.T01:CDS | 35.0% | |
| GTACATAGAAATGGTCACTT+TGG | - | Chr6:111908802-111908821 | MsG0680035762.01.T01:CDS | 35.0% | |
| ! | ACTCTACTCAATTCTCCTTT+TGG | + | Chr6:111908590-111908609 | None:intergenic | 35.0% |
| ! | ATAGAAATGGTCACTTTGGA+TGG | - | Chr6:111908806-111908825 | MsG0680035762.01.T01:CDS | 35.0% |
| ACAAAGAGCAACTTTGTGAG+TGG | + | Chr6:111908540-111908559 | None:intergenic | 40.0% | |
| AGCTTCAAGATATCTCCAAC+AGG | - | Chr6:111908868-111908887 | MsG0680035762.01.T01:CDS | 40.0% | |
| TGTGAGTGGAAATGAAAAGC+AGG | + | Chr6:111908526-111908545 | None:intergenic | 40.0% | |
| ! | ACATGCTGAATCTTTTCGTG+TGG | + | Chr6:111908746-111908765 | None:intergenic | 40.0% |
| !! | GGTTTCGACTTCTGGTTTAT+GGG | - | Chr6:111908827-111908846 | MsG0680035762.01.T01:CDS | 40.0% |
| !! | TGGTTTCGACTTCTGGTTTA+TGG | - | Chr6:111908826-111908845 | MsG0680035762.01.T01:CDS | 40.0% |
| ACAACATCAGGTCCTATTGC+TGG | - | Chr6:111908500-111908519 | MsG0680035762.01.T01:CDS | 45.0% | |
| ATGAAAAGCAGGCCAGCAAT+AGG | + | Chr6:111908515-111908534 | None:intergenic | 45.0% | |
| CTTTGGATGGTTTCGACTTC+TGG | - | Chr6:111908819-111908838 | MsG0680035762.01.T01:CDS | 45.0% | |
| !! | ATAGCGTTGACGATGCATGC+TGG | + | Chr6:111908694-111908713 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 111908437 | 111908901 | 111908437 | ID=MsG0680035762.01;Name=MsG0680035762.01 |
| Chr6 | mRNA | 111908437 | 111908901 | 111908437 | ID=MsG0680035762.01.T01;Parent=MsG0680035762.01;Name=MsG0680035762.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|0|0.5|2|0|116 |
| Chr6 | exon | 111908437 | 111908604 | 111908437 | ID=MsG0680035762.01.T01:exon:9369;Parent=MsG0680035762.01.T01 |
| Chr6 | exon | 111908719 | 111908901 | 111908719 | ID=MsG0680035762.01.T01:exon:9368;Parent=MsG0680035762.01.T01 |
| Chr6 | CDS | 111908719 | 111908901 | 111908719 | ID=MsG0680035762.01.T01:cds;Parent=MsG0680035762.01.T01 |
| Chr6 | CDS | 111908437 | 111908604 | 111908437 | ID=MsG0680035762.01.T01:cds;Parent=MsG0680035762.01.T01 |
| Chr6 | mRNA | 111908437 | 111908526 | 111908437 | ID=MsG0680035762.01.T02;Parent=MsG0680035762.01;Name=MsG0680035762.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|29 |
| Chr6 | exon | 111908437 | 111908526 | 111908437 | ID=MsG0680035762.01.T02:exon:9370;Parent=MsG0680035762.01.T02 |
| Chr6 | CDS | 111908437 | 111908526 | 111908437 | ID=MsG0680035762.01.T02:cds;Parent=MsG0680035762.01.T02 |
| Gene Sequence |
| Protein sequence |
