Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036061.01.T01 | XP_013447445.1 | 98.558 | 208 | 3 | 0 | 1 | 208 | 1 | 208 | 6.82E-147 | 417 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036061.01.T01 | P0C8K6 | 42.857 | 217 | 77 | 5 | 7 | 207 | 8 | 193 | 2.60E-41 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036061.01.T01 | A0A072TWF8 | 98.558 | 208 | 3 | 0 | 1 | 208 | 1 | 208 | 3.26e-147 | 417 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021417.01 | MsG0780036061.01 | 0.807210 | 5.649892e-50 | 3.128328e-47 |
| MsG0380016183.01 | MsG0780036061.01 | 0.815065 | 1.118797e-51 | 7.635017e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036061.01.T01 | MTR_7g007070 | 98.558 | 208 | 3 | 0 | 1 | 208 | 1 | 208 | 8.25e-151 | 417 |
| MsG0780036061.01.T01 | MTR_3g035700 | 53.448 | 58 | 27 | 0 | 21 | 78 | 29 | 86 | 8.25e-14 | 65.9 |
| MsG0780036061.01.T01 | MTR_0002s0350 | 53.448 | 58 | 27 | 0 | 21 | 78 | 82 | 139 | 1.52e-13 | 66.2 |
| MsG0780036061.01.T01 | MTR_4g047920 | 53.448 | 58 | 27 | 0 | 21 | 78 | 143 | 200 | 1.15e-12 | 65.1 |
| MsG0780036061.01.T01 | MTR_4g051430 | 53.448 | 58 | 27 | 0 | 21 | 78 | 143 | 200 | 1.19e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036061.01.T01 | ATMG00560 | 40.873 | 252 | 100 | 11 | 1 | 207 | 1 | 248 | 2.93e-36 | 130 |
| MsG0780036061.01.T01 | AT2G07715 | 40.000 | 250 | 105 | 11 | 1 | 207 | 1 | 248 | 1.24e-35 | 129 |
| MsG0780036061.01.T01 | ATCG00830 | 53.448 | 58 | 27 | 0 | 21 | 78 | 40 | 97 | 4.45e-13 | 66.6 |
| MsG0780036061.01.T01 | ATCG01310 | 53.448 | 58 | 27 | 0 | 21 | 78 | 40 | 97 | 4.45e-13 | 66.6 |
Find 63 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACTCGATGTAAACGTTTA+TGG | 0.211837 | 7:-1968540 | None:intergenic |
| TTTGGATGAATAGGAATAAA+AGG | 0.280173 | 7:+1969004 | MsG0780036061.01.T01:CDS |
| CGAATTTCTCCGTTTGTTGC+AGG | 0.302525 | 7:-1968757 | None:intergenic |
| ATCCACGCTGGAAGTAGTTC+AGG | 0.328737 | 7:+1968814 | MsG0780036061.01.T01:CDS |
| GGCGGCGGTGGCGGTGGTTG+CGG | 0.357152 | 7:-1968689 | None:intergenic |
| GGAGAAATTCGTGGTGTGTT+TGG | 0.372829 | 7:+1968769 | MsG0780036061.01.T01:CDS |
| ATGGTGAGAAATTGGAGATT+AGG | 0.384469 | 7:+1968959 | MsG0780036061.01.T01:CDS |
| GAAAGGGGATGGTGAGAAAT+TGG | 0.384954 | 7:+1968951 | MsG0780036061.01.T01:CDS |
| ATAGTGATGTTTGGATGAAT+AGG | 0.385359 | 7:+1968995 | MsG0780036061.01.T01:CDS |
| AGAAATTGGAGATTAGGAAT+TGG | 0.386894 | 7:+1968965 | MsG0780036061.01.T01:CDS |
| GAATCTGTTTCGTCGGCGTT+AGG | 0.392248 | 7:+1968880 | MsG0780036061.01.T01:CDS |
| CTCGTATCGCTCTCGTTCGT+TGG | 0.411886 | 7:+1968635 | MsG0780036061.01.T01:CDS |
| GGATACAGCGGCGGCGGTGG+CGG | 0.413675 | 7:-1968698 | None:intergenic |
| CTACTTCCAGCGTGGATTTG+TGG | 0.416480 | 7:-1968808 | None:intergenic |
| TACAGCGGCGGCGGTGGCGG+TGG | 0.416978 | 7:-1968695 | None:intergenic |
| AGGATCGTGTTTGAGGAATC+TGG | 0.421010 | 7:-1968737 | None:intergenic |
| GGAAGAAGAATAGTGATGTT+TGG | 0.421549 | 7:+1968986 | MsG0780036061.01.T01:CDS |
| ACCTCCTTCCACCGCGGCGG+CGG | 0.434065 | 7:+1968517 | MsG0780036061.01.T01:CDS |
| GTTGCGGTGCGTTGTGGAGG+TGG | 0.439402 | 7:-1968673 | None:intergenic |
| GGTGGATACAGCGGCGGCGG+TGG | 0.458180 | 7:-1968701 | None:intergenic |
| TGCAGTGGCGGCGTCAAGAC+CGG | 0.462179 | 7:+1968915 | MsG0780036061.01.T01:CDS |
| TTGTTGCAGGATCGTGTTTG+AGG | 0.471831 | 7:-1968744 | None:intergenic |
| CGGTGCGTTGTGGAGGTGGT+TGG | 0.485255 | 7:-1968669 | None:intergenic |
| ATGGCCGCCGCCGCGGTGGA+AGG | 0.490719 | 7:-1968521 | None:intergenic |
| ACCGCCGGCCGTAACTGTTA+CGG | 0.496435 | 7:+1968487 | MsG0780036061.01.T01:CDS |
| GCTCTCGTTCGTTGGCTCAA+CGG | 0.496850 | 7:+1968643 | MsG0780036061.01.T01:CDS |
| TGCGGTGGATACAGCGGCGG+CGG | 0.512467 | 7:-1968704 | None:intergenic |
| GCGGTGGTTGCGGTGCGTTG+TGG | 0.513545 | 7:-1968679 | None:intergenic |
| GCGATACGAGATGAACGGTT+AGG | 0.522070 | 7:-1968622 | None:intergenic |
| TGATGCGGCCGTAACAGTTA+CGG | 0.523351 | 7:-1968495 | None:intergenic |
| GTGGTTGCGGTGCGTTGTGG+AGG | 0.524025 | 7:-1968676 | None:intergenic |
| TTCAGGGAAGGTGTTTCTGT+CGG | 0.527701 | 7:+1968831 | MsG0780036061.01.T01:CDS |
| AAACACGATCCTGCAACAAA+CGG | 0.532110 | 7:+1968748 | MsG0780036061.01.T01:CDS |
| AAAGGAAAACTGCAATTTCG+TGG | 0.550482 | 7:+1969022 | MsG0780036061.01.T01:CDS |
| GCCGCCGCCGCGGTGGAAGG+AGG | 0.550965 | 7:-1968518 | None:intergenic |
| CGCAACACTTCTTCTTCCGT+CGG | 0.562201 | 7:+1968574 | MsG0780036061.01.T01:CDS |
| AGACAATTCACATTCGGTGC+CGG | 0.562885 | 7:+1968460 | MsG0780036061.01.T01:CDS |
| TTTACCGAGGATTGCAGTGG+CGG | 0.564013 | 7:+1968903 | MsG0780036061.01.T01:CDS |
| GACGCCGCCACTGCAATCCT+CGG | 0.564433 | 7:-1968907 | None:intergenic |
| TCACTGAGACAATTCACATT+CGG | 0.564863 | 7:+1968454 | MsG0780036061.01.T01:CDS |
| TCCACGCTGGAAGTAGTTCA+GGG | 0.565983 | 7:+1968815 | MsG0780036061.01.T01:CDS |
| CGCTGGAAGTAGTTCAGGGA+AGG | 0.569007 | 7:+1968819 | MsG0780036061.01.T01:CDS |
| AACGTTTATGGCCGCCGCCG+CGG | 0.570918 | 7:-1968528 | None:intergenic |
| GTGTTTGAGGAATCTGGAGG+AGG | 0.578872 | 7:-1968731 | None:intergenic |
| GCAACAAACGGAGAAATTCG+TGG | 0.583929 | 7:+1968760 | MsG0780036061.01.T01:CDS |
| GTTTATGGCCGCCGCCGCGG+TGG | 0.594084 | 7:-1968525 | None:intergenic |
| CGCGGTGGAAGGAGGTGATG+CGG | 0.598152 | 7:-1968510 | None:intergenic |
| GCCGTAACAGTTACGGCCGG+CGG | 0.598845 | 7:-1968488 | None:intergenic |
| TGAAAGTGAATCTGTTTCGT+CGG | 0.615360 | 7:+1968873 | MsG0780036061.01.T01:CDS |
| AGGTTTACCGAGGATTGCAG+TGG | 0.627488 | 7:+1968900 | MsG0780036061.01.T01:CDS |
| GCGGCCGTAACAGTTACGGC+CGG | 0.631109 | 7:-1968491 | None:intergenic |
| TTGCTTCCACAAATCCACGC+TGG | 0.637501 | 7:+1968802 | MsG0780036061.01.T01:CDS |
| CGTCGGCGTTAGGTTTACCG+AGG | 0.638124 | 7:+1968890 | MsG0780036061.01.T01:CDS |
| TCTGGAGGAGGATGATGCGG+TGG | 0.640620 | 7:-1968719 | None:intergenic |
| GAATCTGGAGGAGGATGATG+CGG | 0.644554 | 7:-1968722 | None:intergenic |
| ATCACCTCCTTCCACCGCGG+CGG | 0.644837 | 7:+1968514 | MsG0780036061.01.T01:CDS |
| GGATGATGCGGTGGATACAG+CGG | 0.673078 | 7:-1968710 | None:intergenic |
| CGAGAGCGATACGAGATGAA+CGG | 0.676738 | 7:-1968627 | None:intergenic |
| TCCCTGAACTACTTCCAGCG+TGG | 0.686694 | 7:-1968816 | None:intergenic |
| CGCATCACCTCCTTCCACCG+CGG | 0.686774 | 7:+1968511 | MsG0780036061.01.T01:CDS |
| GATGCGTTCGACGATACCGA+CGG | 0.688002 | 7:-1968590 | None:intergenic |
| TGATGCGGTGGATACAGCGG+CGG | 0.691932 | 7:-1968707 | None:intergenic |
| ATCGTGTTTGAGGAATCTGG+AGG | 0.723414 | 7:-1968734 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGGATGAATAGGAATAAA+AGG | + | Chr7:1969004-1969023 | MsG0780036061.01.T01:CDS | 25.0% |
| AGAAATTGGAGATTAGGAAT+TGG | + | Chr7:1968965-1968984 | MsG0780036061.01.T01:CDS | 30.0% | |
| ATAGTGATGTTTGGATGAAT+AGG | + | Chr7:1968995-1969014 | MsG0780036061.01.T01:CDS | 30.0% | |
| TAACTCGATGTAAACGTTTA+TGG | - | Chr7:1968543-1968562 | None:intergenic | 30.0% | |
| AAAGGAAAACTGCAATTTCG+TGG | + | Chr7:1969022-1969041 | MsG0780036061.01.T01:CDS | 35.0% | |
| ATGGTGAGAAATTGGAGATT+AGG | + | Chr7:1968959-1968978 | MsG0780036061.01.T01:CDS | 35.0% | |
| GGAAGAAGAATAGTGATGTT+TGG | + | Chr7:1968986-1969005 | MsG0780036061.01.T01:CDS | 35.0% | |
| TCACTGAGACAATTCACATT+CGG | + | Chr7:1968454-1968473 | MsG0780036061.01.T01:CDS | 35.0% | |
| TGAAAGTGAATCTGTTTCGT+CGG | + | Chr7:1968873-1968892 | MsG0780036061.01.T01:CDS | 35.0% | |
| AAACACGATCCTGCAACAAA+CGG | + | Chr7:1968748-1968767 | MsG0780036061.01.T01:CDS | 40.0% | |
| CCTTTCACACCAAAAAACTC+CGG | - | Chr7:1968937-1968956 | None:intergenic | 40.0% | |
| !!! | CGGAGTTTTTTGGTGTGAAA+GGG | + | Chr7:1968935-1968954 | MsG0780036061.01.T01:CDS | 40.0% |
| !!! | GGAGTTTTTTGGTGTGAAAG+GGG | + | Chr7:1968936-1968955 | MsG0780036061.01.T01:CDS | 40.0% |
| !!! | TTTTTTGGTGTGAAAGGGGA+TGG | + | Chr7:1968940-1968959 | MsG0780036061.01.T01:CDS | 40.0% |
| AGGATCGTGTTTGAGGAATC+TGG | - | Chr7:1968740-1968759 | None:intergenic | 45.0% | |
| ATCGTGTTTGAGGAATCTGG+AGG | - | Chr7:1968737-1968756 | None:intergenic | 45.0% | |
| GAAAGGGGATGGTGAGAAAT+TGG | + | Chr7:1968951-1968970 | MsG0780036061.01.T01:CDS | 45.0% | |
| GCAACAAACGGAGAAATTCG+TGG | + | Chr7:1968760-1968779 | MsG0780036061.01.T01:CDS | 45.0% | |
| GGAGAAATTCGTGGTGTGTT+TGG | + | Chr7:1968769-1968788 | MsG0780036061.01.T01:CDS | 45.0% | |
| TTGTTGCAGGATCGTGTTTG+AGG | - | Chr7:1968747-1968766 | None:intergenic | 45.0% | |
| ! | CGAATTTCTCCGTTTGTTGC+AGG | - | Chr7:1968760-1968779 | None:intergenic | 45.0% |
| !! | AGACAATTCACATTCGGTGC+CGG | + | Chr7:1968460-1968479 | MsG0780036061.01.T01:CDS | 45.0% |
| !! | TTCAGGGAAGGTGTTTCTGT+CGG | + | Chr7:1968831-1968850 | MsG0780036061.01.T01:CDS | 45.0% |
| !!! | CCGGAGTTTTTTGGTGTGAA+AGG | + | Chr7:1968934-1968953 | MsG0780036061.01.T01:CDS | 45.0% |
| AGGTTTACCGAGGATTGCAG+TGG | + | Chr7:1968900-1968919 | MsG0780036061.01.T01:CDS | 50.0% | |
| ATCCACGCTGGAAGTAGTTC+AGG | + | Chr7:1968814-1968833 | MsG0780036061.01.T01:CDS | 50.0% | |
| CGAGAGCGATACGAGATGAA+CGG | - | Chr7:1968630-1968649 | None:intergenic | 50.0% | |
| CGCAACACTTCTTCTTCCGT+CGG | + | Chr7:1968574-1968593 | MsG0780036061.01.T01:CDS | 50.0% | |
| CTACTTCCAGCGTGGATTTG+TGG | - | Chr7:1968811-1968830 | None:intergenic | 50.0% | |
| GAATCTGGAGGAGGATGATG+CGG | - | Chr7:1968725-1968744 | None:intergenic | 50.0% | |
| GAATCTGTTTCGTCGGCGTT+AGG | + | Chr7:1968880-1968899 | MsG0780036061.01.T01:CDS | 50.0% | |
| GCGATACGAGATGAACGGTT+AGG | - | Chr7:1968625-1968644 | None:intergenic | 50.0% | |
| GTGTTTGAGGAATCTGGAGG+AGG | - | Chr7:1968734-1968753 | None:intergenic | 50.0% | |
| TCCACGCTGGAAGTAGTTCA+GGG | + | Chr7:1968815-1968834 | MsG0780036061.01.T01:CDS | 50.0% | |
| TGATGCGGCCGTAACAGTTA+CGG | - | Chr7:1968498-1968517 | None:intergenic | 50.0% | |
| TTGCTTCCACAAATCCACGC+TGG | + | Chr7:1968802-1968821 | MsG0780036061.01.T01:CDS | 50.0% | |
| TTTACCGAGGATTGCAGTGG+CGG | + | Chr7:1968903-1968922 | MsG0780036061.01.T01:CDS | 50.0% | |
| !! | GGTGTTTCTGTCGGCGTTTT+CGG | + | Chr7:1968840-1968859 | MsG0780036061.01.T01:CDS | 50.0% |
| !! | GTGTTTCTGTCGGCGTTTTC+GGG | + | Chr7:1968841-1968860 | MsG0780036061.01.T01:CDS | 50.0% |
| !! | TGTTTCTGTCGGCGTTTTCG+GGG | + | Chr7:1968842-1968861 | MsG0780036061.01.T01:CDS | 50.0% |
| !!! | GCGTCAAGACCGGAGTTTTT+TGG | + | Chr7:1968925-1968944 | MsG0780036061.01.T01:CDS | 50.0% |
| CGCTGGAAGTAGTTCAGGGA+AGG | + | Chr7:1968819-1968838 | MsG0780036061.01.T01:CDS | 55.0% | |
| CTCGTATCGCTCTCGTTCGT+TGG | + | Chr7:1968635-1968654 | MsG0780036061.01.T01:CDS | 55.0% | |
| GATGCGTTCGACGATACCGA+CGG | - | Chr7:1968593-1968612 | None:intergenic | 55.0% | |
| GGATGATGCGGTGGATACAG+CGG | - | Chr7:1968713-1968732 | None:intergenic | 55.0% | |
| TCCCTGAACTACTTCCAGCG+TGG | - | Chr7:1968819-1968838 | None:intergenic | 55.0% | |
| !! | GCTCTCGTTCGTTGGCTCAA+CGG | + | Chr7:1968643-1968662 | MsG0780036061.01.T01:CDS | 55.0% |
| ACCGCCGGCCGTAACTGTTA+CGG | + | Chr7:1968487-1968506 | MsG0780036061.01.T01:CDS | 60.0% | |
| CGTCGGCGTTAGGTTTACCG+AGG | + | Chr7:1968890-1968909 | MsG0780036061.01.T01:CDS | 60.0% | |
| TCTGGAGGAGGATGATGCGG+TGG | - | Chr7:1968722-1968741 | None:intergenic | 60.0% | |
| TGATGCGGTGGATACAGCGG+CGG | - | Chr7:1968710-1968729 | None:intergenic | 60.0% | |
| ! | GTTACGGCCGGCGGTTTTTC+CGG | - | Chr7:1968482-1968501 | None:intergenic | 60.0% |
| !! | CTGTCGGCGTTTTCGGGGAA+GGG | + | Chr7:1968847-1968866 | MsG0780036061.01.T01:CDS | 60.0% |
| !! | TCTGTCGGCGTTTTCGGGGA+AGG | + | Chr7:1968846-1968865 | MsG0780036061.01.T01:CDS | 60.0% |
| !! | TTCGGTGCCGGAAAAACCGC+CGG | + | Chr7:1968472-1968491 | MsG0780036061.01.T01:CDS | 60.0% |
| AACGTTTATGGCCGCCGCCG+CGG | - | Chr7:1968531-1968550 | None:intergenic | 65.0% | |
| ATCACCTCCTTCCACCGCGG+CGG | + | Chr7:1968514-1968533 | MsG0780036061.01.T01:CDS | 65.0% | |
| CGCATCACCTCCTTCCACCG+CGG | + | Chr7:1968511-1968530 | MsG0780036061.01.T01:CDS | 65.0% | |
| CGCGGTGGAAGGAGGTGATG+CGG | - | Chr7:1968513-1968532 | None:intergenic | 65.0% | |
| CGGTGCGTTGTGGAGGTGGT+TGG | - | Chr7:1968672-1968691 | None:intergenic | 65.0% | |
| GACGCCGCCACTGCAATCCT+CGG | - | Chr7:1968910-1968929 | None:intergenic | 65.0% | |
| GCCGTAACAGTTACGGCCGG+CGG | - | Chr7:1968491-1968510 | None:intergenic | 65.0% | |
| GCGGCCGTAACAGTTACGGC+CGG | - | Chr7:1968494-1968513 | None:intergenic | 65.0% | |
| GTGGTTGCGGTGCGTTGTGG+AGG | - | Chr7:1968679-1968698 | None:intergenic | 65.0% | |
| GTTGCGGTGCGTTGTGGAGG+TGG | - | Chr7:1968676-1968695 | None:intergenic | 65.0% | |
| TGCAGTGGCGGCGTCAAGAC+CGG | + | Chr7:1968915-1968934 | MsG0780036061.01.T01:CDS | 65.0% | |
| GCGGTGGTTGCGGTGCGTTG+TGG | - | Chr7:1968682-1968701 | None:intergenic | 70.0% | |
| TGCGGTGGATACAGCGGCGG+CGG | - | Chr7:1968707-1968726 | None:intergenic | 70.0% | |
| ACCTCCTTCCACCGCGGCGG+CGG | + | Chr7:1968517-1968536 | MsG0780036061.01.T01:CDS | 75.0% | |
| GGATACAGCGGCGGCGGTGG+CGG | - | Chr7:1968701-1968720 | None:intergenic | 75.0% | |
| GGTGGATACAGCGGCGGCGG+TGG | - | Chr7:1968704-1968723 | None:intergenic | 75.0% | |
| GTTTATGGCCGCCGCCGCGG+TGG | - | Chr7:1968528-1968547 | None:intergenic | 75.0% | |
| ! | ATGGCCGCCGCCGCGGTGGA+AGG | - | Chr7:1968524-1968543 | None:intergenic | 80.0% |
| !! | GGCGGCGGTGGCGGTGGTTG+CGG | - | Chr7:1968692-1968711 | None:intergenic | 80.0% |
| !! | TACAGCGGCGGCGGTGGCGG+TGG | - | Chr7:1968698-1968717 | None:intergenic | 80.0% |
| ! | GCCGCCGCCGCGGTGGAAGG+AGG | - | Chr7:1968521-1968540 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 1968433 | 1969059 | 1968433 | ID=MsG0780036061.01;Name=MsG0780036061.01 |
| Chr7 | mRNA | 1968433 | 1969059 | 1968433 | ID=MsG0780036061.01.T01;Parent=MsG0780036061.01;Name=MsG0780036061.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|208 |
| Chr7 | exon | 1968433 | 1969059 | 1968433 | ID=MsG0780036061.01.T01:exon:38380;Parent=MsG0780036061.01.T01 |
| Chr7 | CDS | 1968433 | 1969059 | 1968433 | ID=MsG0780036061.01.T01:cds;Parent=MsG0780036061.01.T01 |
| Gene Sequence |
| Protein sequence |
