Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038612.01.T01 | XP_003622960.1 | 98.372 | 430 | 7 | 0 | 1 | 430 | 1 | 430 | 0 | 885 |
| MsG0780038612.01.T02 | XP_003622960.1 | 98.372 | 430 | 7 | 0 | 1 | 430 | 1 | 430 | 0 | 885 |
Co-expression Network
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280007071.01 | MsG0780038612.01 | PPI |
| MsG0280007071.01 | MsG0780038612.01 | PPI |
| MsG0580030230.01 | MsG0780038612.01 | PPI |
| MsG0780038612.01 | MsG0580030230.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038612.01.T01 | MTR_7g058810 | 98.372 | 430 | 7 | 0 | 1 | 430 | 1 | 430 | 0.0 | 885 |
| MsG0780038612.01.T01 | MTR_2g019990 | 65.952 | 373 | 114 | 2 | 2 | 370 | 3 | 366 | 4.35e-173 | 494 |
| MsG0780038612.01.T01 | MTR_1g117060 | 55.858 | 478 | 162 | 7 | 1 | 438 | 1 | 469 | 9.30e-173 | 493 |
| MsG0780038612.01.T01 | MTR_7g100500 | 62.434 | 378 | 130 | 5 | 21 | 394 | 7 | 376 | 7.95e-168 | 476 |
| MsG0780038612.01.T01 | MTR_1g067140 | 73.311 | 296 | 79 | 0 | 75 | 370 | 56 | 351 | 2.20e-165 | 470 |
| MsG0780038612.01.T01 | MTR_3g050780 | 59.055 | 381 | 119 | 2 | 1 | 370 | 1 | 355 | 6.66e-162 | 463 |
| MsG0780038612.01.T01 | MTR_7g116660 | 60.500 | 400 | 134 | 5 | 1 | 400 | 1 | 376 | 1.01e-161 | 469 |
| MsG0780038612.01.T01 | MTR_2g095880 | 56.736 | 386 | 153 | 5 | 1 | 381 | 1 | 377 | 3.03e-158 | 453 |
| MsG0780038612.01.T01 | MTR_3g115500 | 57.979 | 376 | 132 | 3 | 1 | 376 | 1 | 350 | 6.21e-156 | 446 |
| MsG0780038612.01.T01 | MTR_5g092120 | 57.181 | 376 | 131 | 5 | 1 | 372 | 1 | 350 | 5.04e-154 | 441 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038612.01.T01 | AT3G07070 | 65.766 | 444 | 114 | 7 | 1 | 436 | 1 | 414 | 0.0 | 565 |
| MsG0780038612.01.T01 | AT3G24790 | 68.901 | 373 | 84 | 4 | 1 | 371 | 1 | 343 | 0.0 | 511 |
| MsG0780038612.01.T01 | AT3G24790 | 79.866 | 298 | 60 | 0 | 74 | 371 | 28 | 325 | 6.00e-179 | 504 |
| MsG0780038612.01.T01 | AT5G13160 | 57.112 | 464 | 145 | 9 | 1 | 431 | 1 | 443 | 3.54e-174 | 496 |
| MsG0780038612.01.T01 | AT4G13190 | 75.490 | 306 | 75 | 0 | 68 | 373 | 46 | 351 | 2.94e-173 | 491 |
| MsG0780038612.01.T01 | AT5G18610 | 65.229 | 371 | 114 | 4 | 2 | 370 | 3 | 360 | 8.24e-172 | 492 |
| MsG0780038612.01.T01 | AT5G18610 | 65.229 | 371 | 114 | 4 | 2 | 370 | 3 | 360 | 8.24e-172 | 492 |
| MsG0780038612.01.T01 | AT5G18610 | 65.229 | 371 | 114 | 4 | 2 | 370 | 3 | 360 | 8.24e-172 | 492 |
| MsG0780038612.01.T01 | AT5G02800 | 72.816 | 309 | 81 | 1 | 75 | 380 | 55 | 363 | 2.13e-167 | 475 |
| MsG0780038612.01.T01 | AT3G20530 | 67.500 | 320 | 103 | 1 | 64 | 382 | 53 | 372 | 6.59e-164 | 467 |
Find 27 sgRNAs with CRISPR-Local
Find 220 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCATCTCAACCTCAGCC+AGG | 0.601278 | 7:-49937929 | MsG0780038612.01.T01:intron |
| AAATTTGGAACAAAGTTTCA+AGG | 0.481879 | 7:+49938358 | None:intergenic |
| AATAACAATAACAATAACAA+TGG | 0.574369 | 7:-49937992 | MsG0780038612.01.T01:CDS |
| AGTGAAAAGAGAAGAATGAG+GGG | 0.702390 | 7:+49938270 | None:intergenic |
| ATAACAATAACAATGGAAAG+AGG | 0.578516 | 7:-49937985 | MsG0780038612.01.T01:CDS |
| ATAGGTTAAAAGTTGGTGCT+AGG | 0.500357 | 7:-49938108 | MsG0780038612.01.T01:intron |
| ATGCTGTTCCTTAGTTTGTT+AGG | 0.276840 | 7:-49938448 | MsG0780038612.01.T02:five_prime_UTR |
| ATTCAAATATATATCTAATC+GGG | 0.361360 | 7:-49938080 | MsG0780038612.01.T01:five_prime_UTR |
| ATTTCATCTTAAAATCATTA+TGG | 0.271985 | 7:-49938386 | MsG0780038612.01.T01:five_prime_UTR |
| CAACAGGGCCTAACAAACTA+AGG | 0.505050 | 7:+49938440 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAGACCAACAAAGATAA+TGG | - | Chr7:49936328-49936347 | MsG0780038612.01.T01:intron | 30.0% | |
| ACAATGTACCTATACATTTG+AGG | - | Chr7:49938468-49938487 | MsG0780038612.01.T02:five_prime_UTR | 30.0% | |
| ACTAGAATATTTGCATGACA+AGG | - | Chr7:49937192-49937211 | MsG0780038612.01.T01:intron | 30.0% | |
| ATATATAATAACACCGACAC+CGG | + | Chr7:49936119-49936138 | None:intergenic | 30.0% | |
| ATCTTTGTCCAATAAGATGT+TGG | + | Chr7:49937254-49937273 | None:intergenic | 30.0% | |
| ATTCTTGTGAACCAATCTAA+TGG | + | Chr7:49937145-49937164 | None:intergenic | 30.0% | |
| CACAAGAATGAAAATAGCAT+TGG | - | Chr7:49937156-49937175 | MsG0780038612.01.T01:intron | 30.0% | |
| CATACATAAGCTATGAAACA+CGG | - | Chr7:49935968-49935987 | MsG0780038612.01.T01:intron | 30.0% | |
| CTAATTCTGGATATCTTTGT+GGG | + | Chr7:49938050-49938069 | None:intergenic | 30.0% | |
| GGATTTGGAAGAGTTTATAA+AGG | - | Chr7:49936439-49936458 | MsG0780038612.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 49935384 | 49938498 | 49935384 | ID=MsG0780038612.01;Name=MsG0780038612.01 |
| Chr7 | mRNA | 49935384 | 49938450 | 49935384 | ID=MsG0780038612.01.T01;Parent=MsG0780038612.01;Name=MsG0780038612.01.T01;_AED=0.24;_eAED=0.24;_QI=340|1|1|1|0.8|0.66|6|88|438 |
| Chr7 | exon | 49937930 | 49938126 | 49937930 | ID=MsG0780038612.01.T01:exon:6780;Parent=MsG0780038612.01.T01 |
| Chr7 | exon | 49938166 | 49938450 | 49938166 | ID=MsG0780038612.01.T01:exon:6779;Parent=MsG0780038612.01.T01 |
| Chr7 | five_prime_UTR | 49938166 | 49938450 | 49938166 | ID=MsG0780038612.01.T01:five_prime_utr;Parent=MsG0780038612.01.T01 |
| Chr7 | five_prime_UTR | 49938072 | 49938126 | 49938072 | ID=MsG0780038612.01.T01:five_prime_utr;Parent=MsG0780038612.01.T01 |
| Chr7 | CDS | 49937930 | 49938071 | 49937930 | ID=MsG0780038612.01.T01:cds;Parent=MsG0780038612.01.T01 |
| Chr7 | CDS | 49937399 | 49937607 | 49937399 | ID=MsG0780038612.01.T01:cds;Parent=MsG0780038612.01.T01 |
| Chr7 | CDS | 49936951 | 49937140 | 49936951 | ID=MsG0780038612.01.T01:cds;Parent=MsG0780038612.01.T01 |
| Chr7 | CDS | 49936369 | 49936760 | 49936369 | ID=MsG0780038612.01.T01:cds;Parent=MsG0780038612.01.T01 |
| Chr7 | CDS | 49935472 | 49935855 | 49935472 | ID=MsG0780038612.01.T01:cds;Parent=MsG0780038612.01.T01 |
| Chr7 | three_prime_UTR | 49935384 | 49935471 | 49935384 | ID=MsG0780038612.01.T01:three_prime_utr;Parent=MsG0780038612.01.T01 |
| Chr7 | mRNA | 49935384 | 49938498 | 49935384 | ID=MsG0780038612.01.T02;Parent=MsG0780038612.01;Name=MsG0780038612.01.T02;_AED=0.22;_eAED=0.22;_QI=427|1|1|1|1|1|5|88|438 |
| Chr7 | exon | 49937930 | 49938498 | 49937930 | ID=MsG0780038612.01.T02:exon:6785;Parent=MsG0780038612.01.T02 |
| Chr7 | five_prime_UTR | 49938072 | 49938498 | 49938072 | ID=MsG0780038612.01.T02:five_prime_utr;Parent=MsG0780038612.01.T02 |
| Chr7 | CDS | 49937930 | 49938071 | 49937930 | ID=MsG0780038612.01.T02:cds;Parent=MsG0780038612.01.T02 |
| Chr7 | CDS | 49937399 | 49937607 | 49937399 | ID=MsG0780038612.01.T02:cds;Parent=MsG0780038612.01.T02 |
| Chr7 | CDS | 49936951 | 49937140 | 49936951 | ID=MsG0780038612.01.T02:cds;Parent=MsG0780038612.01.T02 |
| Chr7 | CDS | 49936369 | 49936760 | 49936369 | ID=MsG0780038612.01.T02:cds;Parent=MsG0780038612.01.T02 |
| Chr7 | CDS | 49935472 | 49935855 | 49935472 | ID=MsG0780038612.01.T02:cds;Parent=MsG0780038612.01.T02 |
| Chr7 | three_prime_UTR | 49935384 | 49935471 | 49935384 | ID=MsG0780038612.01.T02:three_prime_utr;Parent=MsG0780038612.01.T02 |
| Gene Sequence |
>MsG0780038612.01.T01 ATGAGTTGCTTTTCATGTTTCTCGTCCCAAGAAAAGAAACTATCAAAGAGACCTAACAATAACAATAACAATAACAATGGAAAGAGGATACAACATCATCCTACTCAAATAACACCTCAGAAAGCATCTCAACCTCAGCCAGAGAATCAGTATCACAAGGCAAGGGCAAACCCACCAACAGAACCTAAAATAAATAAAGAGACCAACAAAGATAATGGTCACAACATTGCTGCACAAACGTTCACCTTCAGGGAATTGGCTGCAATCACAAGAAACTTTAGGCAGGAAAATCTAATTGGTGAAGGTGGATTTGGAAGAGTTTATAAAGGAAGACTTGAAAAAACCAACCAGGAAGTAGCTGTGAAGCAACTTGACAGGAATGGATTGCAAGGAAACCGAGAGTTTCTTGTTGAAGTGTTGATGTTGAGCCTTTTACATCATAAAAATCTAGTTAATCTAATTGGATATTGTGCTGATGGAGATCAAAGACTATTGGTATATGAGTTCATGTCATTAGGATCTCTTGAGGATCATCTACTTGATCTTGAACCACAACAAAAGCCATTAGATTGGTTCACAAGAATGAAAATAGCATTGGATGCTGCAAAAGGACTAGAATATTTGCATGACAAGGCCAATCCACCTGTCATATACCGTGACTTAAAATCTTCCAACATCTTATTGGACAAAGATTTCAATGCAAAACTCTCTGATTTTGGACTAGCAAAGCTGGGACCAACAGGGGATATGTCTCATGTGTCTTCAAGAGTAATGGGGACTTATGGATATTGTGCACCTGAGTATCAAAGAACAGGTCAACTCACTGTGAAATCAGATATATACAGTTTTGGTGTTGTTTTGCTTGAATTGATTACTGGAAGGAGAACTATTGATAACACAAGACCTTCAAGGGAGCAAAATCTTGTTTCTTGGTCATATCCAGTATTCAAGGACCCACAAAGATATCCAGAATTAGCAGATCCAAAACTTGAAGGAAACTTTCCAATGAGATCATTACATCAAGCAGTGGCAGTAGCAGCTATGTGTCTAAATGAAGAACCATCAGTGAGACCATTGATAAGTGATGTTGTAACAGCTCTTAGTTTCCTTGGAGTAGATCCAATGAATCAAGATCCACAAGTTTTGTCACCAATTGACATGCCATCACCAACACAAAAGAATGAAGAAAGTAGTGCAACCTTGAGTCTTCTCGACGATGATAGTGTCGTTGAACGCCAAAAAGCCGTTGATGAAGCCATGGAATGGGGTTCAAATACTAGAAATAAACCAAGGCCTGACAGTTCTTCATCTTTGTAA >MsG0780038612.01.T02 ATGAGTTGCTTTTCATGTTTCTCGTCCCAAGAAAAGAAACTATCAAAGAGACCTAACAATAACAATAACAATAACAATGGAAAGAGGATACAACATCATCCTACTCAAATAACACCTCAGAAAGCATCTCAACCTCAGCCAGAGAATCAGTATCACAAGGCAAGGGCAAACCCACCAACAGAACCTAAAATAAATAAAGAGACCAACAAAGATAATGGTCACAACATTGCTGCACAAACGTTCACCTTCAGGGAATTGGCTGCAATCACAAGAAACTTTAGGCAGGAAAATCTAATTGGTGAAGGTGGATTTGGAAGAGTTTATAAAGGAAGACTTGAAAAAACCAACCAGGAAGTAGCTGTGAAGCAACTTGACAGGAATGGATTGCAAGGAAACCGAGAGTTTCTTGTTGAAGTGTTGATGTTGAGCCTTTTACATCATAAAAATCTAGTTAATCTAATTGGATATTGTGCTGATGGAGATCAAAGACTATTGGTATATGAGTTCATGTCATTAGGATCTCTTGAGGATCATCTACTTGATCTTGAACCACAACAAAAGCCATTAGATTGGTTCACAAGAATGAAAATAGCATTGGATGCTGCAAAAGGACTAGAATATTTGCATGACAAGGCCAATCCACCTGTCATATACCGTGACTTAAAATCTTCCAACATCTTATTGGACAAAGATTTCAATGCAAAACTCTCTGATTTTGGACTAGCAAAGCTGGGACCAACAGGGGATATGTCTCATGTGTCTTCAAGAGTAATGGGGACTTATGGATATTGTGCACCTGAGTATCAAAGAACAGGTCAACTCACTGTGAAATCAGATATATACAGTTTTGGTGTTGTTTTGCTTGAATTGATTACTGGAAGGAGAACTATTGATAACACAAGACCTTCAAGGGAGCAAAATCTTGTTTCTTGGTCATATCCAGTATTCAAGGACCCACAAAGATATCCAGAATTAGCAGATCCAAAACTTGAAGGAAACTTTCCAATGAGATCATTACATCAAGCAGTGGCAGTAGCAGCTATGTGTCTAAATGAAGAACCATCAGTGAGACCATTGATAAGTGATGTTGTAACAGCTCTTAGTTTCCTTGGAGTAGATCCAATGAATCAAGATCCACAAGTTTTGTCACCAATTGACATGCCATCACCAACACAAAAGAATGAAGAAAGTAGTGCAACCTTGAGTCTTCTCGACGATGATAGTGTCGTTGAACGCCAAAAAGCCGTTGATGAAGCCATGGAATGGGGTTCAAATACTAGAAATAAACCAAGGCCTGACAGTTCTTCATCTTTGTAA |
| Protein sequence |
>MsG0780038612.01.T01 MSCFSCFSSQEKKLSKRPNNNNNNNNGKRIQHHPTQITPQKASQPQPENQYHKARANPPTEPKINKETNKDNGHNIAAQTFTFRELAAITRNFRQENLIGEGGFGRVYKGRLEKTNQEVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEFMSLGSLEDHLLDLEPQQKPLDWFTRMKIALDAAKGLEYLHDKANPPVIYRDLKSSNILLDKDFNAKLSDFGLAKLGPTGDMSHVSSRVMGTYGYCAPEYQRTGQLTVKSDIYSFGVVLLELITGRRTIDNTRPSREQNLVSWSYPVFKDPQRYPELADPKLEGNFPMRSLHQAVAVAAMCLNEEPSVRPLISDVVTALSFLGVDPMNQDPQVLSPIDMPSPTQKNEESSATLSLLDDDSVVERQKAVDEAMEWGSNTRNKPRPDSSSSL* >MsG0780038612.01.T02 MSCFSCFSSQEKKLSKRPNNNNNNNNGKRIQHHPTQITPQKASQPQPENQYHKARANPPTEPKINKETNKDNGHNIAAQTFTFRELAAITRNFRQENLIGEGGFGRVYKGRLEKTNQEVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEFMSLGSLEDHLLDLEPQQKPLDWFTRMKIALDAAKGLEYLHDKANPPVIYRDLKSSNILLDKDFNAKLSDFGLAKLGPTGDMSHVSSRVMGTYGYCAPEYQRTGQLTVKSDIYSFGVVLLELITGRRTIDNTRPSREQNLVSWSYPVFKDPQRYPELADPKLEGNFPMRSLHQAVAVAAMCLNEEPSVRPLISDVVTALSFLGVDPMNQDPQVLSPIDMPSPTQKNEESSATLSLLDDDSVVERQKAVDEAMEWGSNTRNKPRPDSSSSL* |
