AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038796.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038796.01.T01 MTR_3g077410 89.412 85 9 0 1 85 75 159 4.21e-47 154
MsG0780038796.01.T01 MTR_4g105310 74.118 85 22 0 1 85 86 170 2.35e-38 130
MsG0780038796.01.T01 MTR_4g105310 74.118 85 22 0 1 85 86 170 1.17e-37 130
MsG0780038796.01.T01 MTR_6g053600 56.098 82 36 0 3 84 63 144 6.56e-22 87.8
MsG0780038796.01.T01 MTR_1g100305 50.000 82 41 0 3 84 69 150 2.79e-21 86.3
MsG0780038796.01.T01 MTR_6g053550 54.878 82 37 0 3 84 77 158 1.67e-20 84.3
MsG0780038796.01.T01 MTR_7g099850 52.381 84 40 0 2 85 73 156 3.19e-20 83.6
MsG0780038796.01.T01 MTR_8g099995 48.810 84 43 0 2 85 74 157 5.28e-20 83.2
MsG0780038796.01.T01 MTR_1g100395 53.659 82 38 0 3 84 69 150 1.46e-19 81.6
MsG0780038796.01.T01 MTR_1g100315 51.220 82 40 0 3 84 69 150 2.32e-19 81.3
MsG0780038796.01.T01 MTR_8g098415 48.780 82 42 0 3 84 69 150 5.67e-19 78.6
MsG0780038796.01.T01 MTR_8g098415 48.780 82 42 0 3 84 12 93 8.26e-19 79.0
MsG0780038796.01.T01 MTR_1g100335 51.220 82 40 0 3 84 69 150 1.00e-18 79.0
MsG0780038796.01.T01 MTR_8g098415 48.780 82 42 0 3 84 69 150 1.31e-18 79.3
MsG0780038796.01.T01 MTR_8g098415 48.780 82 42 0 3 84 69 150 1.34e-18 79.3
MsG0780038796.01.T01 MTR_1g100335 51.220 82 40 0 3 84 69 150 1.40e-18 78.6
MsG0780038796.01.T01 MTR_1g100335 51.220 82 40 0 3 84 69 150 1.70e-18 79.0
MsG0780038796.01.T01 MTR_1g068970 50.633 79 39 0 7 85 11 89 4.06e-18 77.0
MsG0780038796.01.T01 MTR_1g068970 50.633 79 39 0 7 85 78 156 7.99e-18 77.0
MsG0780038796.01.T01 MTR_8g022500 82.927 41 7 0 38 78 476 516 6.96e-16 72.4
MsG0780038796.01.T02 MTR_3g077410 81.928 83 15 0 1 83 75 157 1.17e-40 136
MsG0780038796.01.T02 MTR_4g105310 68.675 83 26 0 1 83 86 168 6.18e-33 114
MsG0780038796.01.T02 MTR_4g105310 68.675 83 26 0 1 83 86 168 3.01e-32 114
MsG0780038796.01.T02 MTR_6g053600 51.852 81 39 0 3 83 63 143 7.35e-19 78.6
MsG0780038796.01.T02 MTR_8g022500 84.091 44 7 0 38 81 476 519 1.03e-18 79.0
MsG0780038796.01.T02 MTR_1g100305 45.679 81 44 0 3 83 69 149 3.04e-18 77.0
MsG0780038796.01.T02 MTR_6g053550 50.617 81 40 0 3 83 77 157 7.32e-18 75.9
MsG0780038796.01.T02 MTR_1g100395 49.383 81 41 0 3 83 69 149 5.92e-17 73.6
MsG0780038796.01.T02 MTR_1g100315 47.500 80 42 0 4 83 70 149 9.37e-17 72.8
MsG0780038796.01.T02 MTR_8g098415 44.578 83 46 0 1 83 67 149 1.15e-16 72.8
MsG0780038796.01.T02 MTR_8g098415 44.578 83 46 0 1 83 67 149 1.22e-16 72.8
MsG0780038796.01.T02 MTR_7g099850 47.561 82 43 0 2 83 73 154 2.03e-16 72.0
MsG0780038796.01.T02 MTR_8g098415 46.154 78 42 0 3 80 69 146 2.96e-16 70.5
MsG0780038796.01.T02 MTR_8g098415 46.154 78 42 0 3 80 12 89 4.24e-16 70.5
MsG0780038796.01.T02 MTR_1g100335 46.914 81 43 0 3 83 69 149 6.01e-16 70.9
MsG0780038796.01.T02 MTR_8g099995 45.455 77 42 0 2 78 74 150 1.46e-15 69.7
MsG0780038796.01.T02 MTR_1g100335 47.436 78 41 0 3 80 69 146 1.49e-15 69.3
MsG0780038796.01.T02 MTR_1g100335 47.436 78 41 0 3 80 69 146 1.87e-15 68.9
MsG0780038796.01.T02 MTR_1g068970 47.297 74 39 0 7 80 11 84 1.27e-14 66.6
MsG0780038796.01.T02 MTR_1g068970 47.297 74 39 0 7 80 78 151 2.01e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 76 158 7.50e-31 110
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 76 158 8.17e-31 110
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 43 125 2.91e-30 110
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 43 125 3.12e-30 110
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 43 125 3.56e-30 109
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 43 125 3.88e-30 109
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 76 158 4.17e-30 110
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 76 158 5.22e-30 109
MsG0780038796.01.T01 AT5G50130 65.060 83 29 0 3 85 76 158 5.70e-30 109
MsG0780038796.01.T01 AT4G11410 51.190 84 41 0 2 85 68 151 4.14e-22 87.8
MsG0780038796.01.T01 AT4G11410 51.190 84 41 0 2 85 68 151 8.12e-22 87.8
MsG0780038796.01.T01 AT4G24050 42.056 107 62 0 2 108 73 179 3.01e-21 85.9
MsG0780038796.01.T01 AT4G24050 50.000 84 42 0 2 85 73 156 5.59e-21 85.9
MsG0780038796.01.T01 AT4G23420 49.398 83 42 0 2 84 68 150 3.54e-20 82.8
MsG0780038796.01.T01 AT4G23420 49.398 83 42 0 2 84 68 150 3.83e-20 82.8
MsG0780038796.01.T01 AT4G23430 49.398 83 42 0 2 84 68 150 4.19e-20 83.6
MsG0780038796.01.T01 AT4G23430 49.398 83 42 0 2 84 68 150 4.33e-20 83.2
MsG0780038796.01.T01 AT4G23420 49.398 83 42 0 2 84 68 150 6.20e-20 82.8
MsG0780038796.01.T01 AT4G23420 49.398 83 42 0 2 84 68 150 6.20e-20 82.8
MsG0780038796.01.T01 AT4G23420 49.398 83 42 0 2 84 85 167 6.89e-20 82.8
MsG0780038796.01.T01 AT2G37540 51.220 82 40 0 4 85 74 155 3.49e-19 80.1
MsG0780038796.01.T01 AT2G37540 51.220 82 40 0 4 85 74 155 9.18e-19 79.7
MsG0780038796.01.T01 AT1G64590 49.333 75 38 0 2 76 73 147 1.30e-17 76.6
MsG0780038796.01.T01 AT5G02540 46.341 82 44 0 3 84 73 154 2.20e-17 76.3
MsG0780038796.01.T01 AT4G23430 35.000 100 65 0 2 101 68 167 4.30e-13 64.3
MsG0780038796.01.T01 AT4G23430 35.000 100 65 0 2 101 68 167 4.43e-13 63.9
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 76 151 5.01e-26 96.7
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 76 151 5.22e-26 96.7
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 43 118 5.31e-25 94.7
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 43 118 5.33e-25 94.7
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 43 118 6.29e-25 94.4
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 43 118 6.67e-25 94.4
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 76 151 6.75e-25 95.1
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 76 151 8.82e-25 94.7
MsG0780038796.01.T02 AT5G50130 61.842 76 29 0 3 78 76 151 9.03e-25 94.7
MsG0780038796.01.T02 AT4G11410 48.780 82 42 0 2 83 68 149 7.48e-20 81.3
MsG0780038796.01.T02 AT4G11410 49.367 79 40 0 2 80 68 146 4.60e-19 78.6
MsG0780038796.01.T02 AT4G24050 46.341 82 44 0 2 83 73 154 1.86e-18 77.8
MsG0780038796.01.T02 AT4G24050 46.341 82 44 0 2 83 73 154 3.04e-18 76.6
MsG0780038796.01.T02 AT1G64590 48.000 75 39 0 2 76 73 147 3.73e-17 74.3
MsG0780038796.01.T02 AT4G23430 45.455 77 42 0 2 78 68 144 3.68e-16 71.2
MsG0780038796.01.T02 AT4G23430 45.455 77 42 0 2 78 68 144 3.73e-16 71.2
MsG0780038796.01.T02 AT4G23420 45.455 77 42 0 2 78 68 144 4.06e-16 70.9
MsG0780038796.01.T02 AT4G23420 45.455 77 42 0 2 78 68 144 5.41e-16 70.5
MsG0780038796.01.T02 AT4G23420 45.455 77 42 0 2 78 68 144 6.27e-16 70.9
MsG0780038796.01.T02 AT4G23420 45.455 77 42 0 2 78 68 144 6.27e-16 70.9
MsG0780038796.01.T02 AT4G23420 45.455 77 42 0 2 78 85 161 6.92e-16 70.9
MsG0780038796.01.T02 AT2G37540 46.250 80 43 0 4 83 74 153 1.51e-15 69.3
MsG0780038796.01.T02 AT2G37540 46.250 80 43 0 4 83 74 153 2.15e-15 69.3
MsG0780038796.01.T02 AT5G02540 42.500 80 46 0 4 83 74 153 1.55e-14 67.0
MsG0780038796.01.T02 AT4G23430 44.595 74 41 0 2 75 68 141 2.42e-14 66.2
MsG0780038796.01.T02 AT4G23430 44.595 74 41 0 2 75 68 141 2.44e-14 66.2

Find 10 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAATTCTTAATGCATATT+TGG 0.143970 7:-53228330 MsG0780038796.01.T01:intron
TTTGCTAACAGAAATGTTAT+TGG 0.226965 7:-53228246 MsG0780038796.01.T01:CDS
GATATTTGCCACAAATTACT+TGG 0.390105 7:-53228360 MsG0780038796.01.T01:CDS
ATGCATATTTGGTATTATAG+TGG 0.400541 7:-53228319 MsG0780038796.01.T01:intron
AAATGTACCCAAGTAATTTG+TGG 0.453221 7:+53228352 None:intergenic
GCATTCCGGATTACGTAATC+CGG 0.471491 7:+53228138 None:intergenic
TTCATGGTGCTAATGCAATC+CGG 0.482941 7:-53228157 MsG0780038796.01.T01:CDS
GAGGGCGGAGTTCAGTAAGA+AGG 0.516127 7:+53228213 None:intergenic
ATATTTGCCACAAATTACTT+GGG 0.538228 7:-53228359 MsG0780038796.01.T01:intron
AGGGCGGAGTTCAGTAAGAA+GGG 0.590492 7:+53228214 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATATTTTATTGAATTGT+AGG - Chr7:53228556-53228575 MsG0780038796.01.T01:intron 15.0%
!! ACTTACATTAGAATATTAAG+TGG + Chr7:53228274-53228293 None:intergenic 20.0%
!! TATAGCATAGTGAAATAAAA+TGG - Chr7:53228331-53228350 MsG0780038796.01.T01:intron 20.0%
!! TCTAATTCTTAATGCATATT+TGG - Chr7:53228501-53228520 MsG0780038796.01.T01:intron 20.0%
! ATATTTGCCACAAATTACTT+GGG - Chr7:53228472-53228491 MsG0780038796.01.T01:intron 25.0%
! ATGCATATTTGGTATTATAG+TGG - Chr7:53228512-53228531 MsG0780038796.01.T01:intron 25.0%
! TTTGCTAACAGAAATGTTAT+TGG - Chr7:53228585-53228604 MsG0780038796.01.T01:CDS 25.0%
!! AAATTTTACAGAAACAATGC+TGG - Chr7:53228403-53228422 MsG0780038796.01.T01:CDS 25.0%
!! GATGGAATTTTAGATTTTGA+GGG + Chr7:53228639-53228658 None:intergenic 25.0%
!! TGATGGAATTTTAGATTTTG+AGG + Chr7:53228640-53228659 None:intergenic 25.0%
AAATGTACCCAAGTAATTTG+TGG + Chr7:53228482-53228501 None:intergenic 30.0%
CATTAGCACCATGAAAAAAA+GGG + Chr7:53228669-53228688 None:intergenic 30.0%
GAAAAAAAGGGTCTAAATGA+TGG + Chr7:53228657-53228676 None:intergenic 30.0%
GATATTTGCCACAAATTACT+TGG - Chr7:53228471-53228490 MsG0780038796.01.T01:intron 30.0%
!!! CATTTAGACCCTTTTTTTCA+TGG - Chr7:53228658-53228677 MsG0780038796.01.T01:CDS 30.0%
AAAGGAATAACCTCAGCATT+TGG + Chr7:53228184-53228203 None:intergenic 35.0%
AAATGCTGAGGTTATTCCTT+TGG - Chr7:53228183-53228202 MsG0780038796.01.T01:CDS 35.0%
AAGCTAAGATCAATCTCCAA+AGG + Chr7:53228202-53228221 None:intergenic 35.0%
GCATTAGCACCATGAAAAAA+AGG + Chr7:53228670-53228689 None:intergenic 35.0%
!! GGAATTTTAGATTTTGAGGG+CGG + Chr7:53228636-53228655 None:intergenic 35.0%
AAAAGAGAGTCCAAATGCTG+AGG - Chr7:53228171-53228190 MsG0780038796.01.T01:CDS 40.0%
! TTCATGGTGCTAATGCAATC+CGG - Chr7:53228674-53228693 MsG0780038796.01.T01:CDS 40.0%
AGGGCGGAGTTCAGTAAGAA+GGG + Chr7:53228620-53228639 None:intergenic 50.0%
GAGGGCGGAGTTCAGTAAGA+AGG + Chr7:53228621-53228640 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 53228148 53228705 53228148 ID=MsG0780038796.01;Name=MsG0780038796.01
Chr7 mRNA 53228148 53228705 53228148 ID=MsG0780038796.01.T01;Parent=MsG0780038796.01;Name=MsG0780038796.01.T01;_AED=0.59;_eAED=0.59;_QI=0|0|0|1|0|0|3|0|115
Chr7 exon 53228148 53228275 53228148 ID=MsG0780038796.01.T01:exon:8515;Parent=MsG0780038796.01.T01
Chr7 exon 53228360 53228439 53228360 ID=MsG0780038796.01.T01:exon:8514;Parent=MsG0780038796.01.T01
Chr7 CDS 53228566 53228705 53228566 ID=MsG0780038796.01.T01:cds;Parent=MsG0780038796.01.T01
Chr7 CDS 53228360 53228439 53228360 ID=MsG0780038796.01.T01:cds;Parent=MsG0780038796.01.T01
Chr7 CDS 53228148 53228275 53228148 ID=MsG0780038796.01.T01:cds;Parent=MsG0780038796.01.T01
Chr7 mRNA 53228322 53228705 53228322 ID=MsG0780038796.01.T02;Parent=MsG0780038796.01;Name=MsG0780038796.01.T02;_AED=0.45;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|85
Chr7 exon 53228322 53228439 53228322 ID=MsG0780038796.01.T02:exon:8516;Parent=MsG0780038796.01.T02
Chr7 CDS 53228566 53228705 53228566 ID=MsG0780038796.01.T02:cds;Parent=MsG0780038796.01.T02
Chr7 CDS 53228322 53228439 53228322 ID=MsG0780038796.01.T02:cds;Parent=MsG0780038796.01.T02
Gene Sequence

>MsG0780038796.01.T01

ATGAAAGTGAGAGACAATATACAAAAAGAGAGTCCAAATGCTGAGGTTATTCCTTTGGAGATTGATCTTAGCTTTTTTTCTTCTGTACAAATATTTTGTTCTGATTTTTTAGCTTTAGATCGTCCACTTAATATTCTAATAAACAATGCTGGTATATTCTCTCGAGTTTTAGAGTTTTCTGCAGAGAAAATTGAGATGATATTTGCCACAAATTACTTGGGACATTTTTTGCTAACAGAAATGTTATTGGACAAAAAGTCCCTTCTTACTGAACTCCGCCCTCAAAATCTAAAATTCCATCATTTAGACCCTTTTTTTCATGGTGCTAATGCAATCCGGATTACGTAA

>MsG0780038796.01.T02

ATGAAAGTGAGAGACAATATACAAAAAGAGAGTCCAAATGCTGAGGTTATTCCTTTGGAGATTGATCTTAGCTTTTTTTCTTCTGTACAAATATTTTGTTCTGATTTTTTAGCTTTAGATCGTCCACTTAATATTCTAATAAACAATGCTGGTATATTCTCTCGAGTTTTAGAGTTTTCTGCAGAGAAAATTGAGATGATATTTGCCACAAATTACTTGGGTACATTTCTAATTCTTAATGCATATTTGGTATTATAG

Protein sequence

>MsG0780038796.01.T01

MKVRDNIQKESPNAEVIPLEIDLSFFSSVQIFCSDFLALDRPLNILINNAGIFSRVLEFSAEKIEMIFATNYLGHFLLTEMLLDKKSLLTELRPQNLKFHHLDPFFHGANAIRIT*

>MsG0780038796.01.T02

MKVRDNIQKESPNAEVIPLEIDLSFFSSVQIFCSDFLALDRPLNILINNAGIFSRVLEFSAEKIEMIFATNYLGTFLILNAYLVL*