Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038953.01.T01 | XP_013443256.1 | 96.407 | 167 | 6 | 0 | 1 | 167 | 1 | 167 | 2.15E-116 | 336 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038953.01.T01 | P05478 | 44.848 | 165 | 86 | 2 | 1 | 165 | 1 | 160 | 1.08E-42 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038953.01.T01 | I3S4N2 | 96.407 | 167 | 6 | 0 | 1 | 167 | 1 | 167 | 1.02e-116 | 336 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012960.01 | MsG0780038953.01 | 0.811130 | 8.164625e-51 | 5.013093e-48 |
| MsG0380017710.01 | MsG0780038953.01 | 0.800638 | 1.312288e-48 | 6.141400e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038953.01.T01 | MTR_0027s0230 | 96.407 | 167 | 6 | 0 | 1 | 167 | 1 | 167 | 2.60e-120 | 336 |
| MsG0780038953.01.T01 | MTR_4g091590 | 46.667 | 165 | 81 | 2 | 1 | 165 | 1 | 158 | 2.48e-45 | 146 |
| MsG0780038953.01.T01 | MTR_6g061940 | 45.455 | 165 | 83 | 2 | 1 | 165 | 1 | 158 | 3.32e-44 | 143 |
| MsG0780038953.01.T01 | MTR_6g061850 | 46.061 | 165 | 82 | 2 | 1 | 165 | 1 | 158 | 1.84e-43 | 142 |
| MsG0780038953.01.T01 | MTR_4g088490 | 43.976 | 166 | 86 | 2 | 1 | 165 | 1 | 160 | 1.51e-42 | 139 |
| MsG0780038953.01.T01 | MTR_4g088475 | 44.118 | 170 | 82 | 3 | 1 | 165 | 1 | 162 | 3.96e-40 | 135 |
| MsG0780038953.01.T01 | MTR_4g088475 | 34.591 | 159 | 87 | 3 | 8 | 165 | 88 | 230 | 3.02e-23 | 92.0 |
| MsG0780038953.01.T01 | MTR_5g081530 | 48.739 | 119 | 61 | 0 | 47 | 165 | 31 | 149 | 1.04e-34 | 119 |
| MsG0780038953.01.T01 | MTR_4g088445 | 46.610 | 118 | 63 | 0 | 39 | 156 | 13 | 130 | 8.59e-34 | 116 |
| MsG0780038953.01.T01 | MTR_5g064060 | 44.578 | 166 | 74 | 3 | 1 | 165 | 1 | 149 | 7.99e-33 | 114 |
| MsG0780038953.01.T01 | MTR_6g022000 | 42.105 | 133 | 74 | 2 | 35 | 164 | 4 | 136 | 1.63e-28 | 103 |
| MsG0780038953.01.T01 | MTR_4g093080 | 47.059 | 102 | 54 | 0 | 64 | 165 | 675 | 776 | 5.51e-28 | 109 |
| MsG0780038953.01.T01 | MTR_4g088450 | 42.063 | 126 | 66 | 2 | 1 | 125 | 1 | 120 | 2.01e-26 | 97.4 |
| MsG0780038953.01.T01 | MTR_1g076880 | 39.450 | 109 | 65 | 1 | 51 | 158 | 66 | 174 | 6.14e-22 | 87.8 |
| MsG0780038953.01.T01 | MTR_1g076860 | 37.736 | 106 | 63 | 1 | 60 | 162 | 69 | 174 | 7.53e-22 | 87.4 |
| MsG0780038953.01.T01 | MTR_1g076910 | 38.835 | 103 | 62 | 1 | 50 | 151 | 68 | 170 | 1.14e-20 | 84.3 |
| MsG0780038953.01.T01 | MTR_4g093140 | 43.689 | 103 | 50 | 3 | 48 | 149 | 700 | 795 | 2.80e-20 | 87.0 |
| MsG0780038953.01.T01 | MTR_1g076890 | 38.462 | 91 | 56 | 0 | 61 | 151 | 70 | 160 | 2.07e-18 | 78.6 |
| MsG0780038953.01.T01 | MTR_2g063560 | 37.179 | 156 | 69 | 4 | 1 | 151 | 1 | 132 | 2.13e-18 | 78.2 |
| MsG0780038953.01.T01 | MTR_1g075470 | 37.383 | 107 | 64 | 1 | 60 | 163 | 42 | 148 | 2.39e-18 | 77.8 |
| MsG0780038953.01.T01 | MTR_1g076900 | 37.234 | 94 | 59 | 0 | 58 | 151 | 62 | 155 | 2.49e-18 | 77.8 |
| MsG0780038953.01.T01 | MTR_4g064993 | 33.929 | 112 | 55 | 2 | 57 | 167 | 56 | 149 | 1.48e-16 | 72.8 |
| MsG0780038953.01.T01 | MTR_5g078040 | 33.929 | 112 | 64 | 4 | 61 | 165 | 31 | 139 | 1.84e-14 | 67.0 |
| MsG0780038953.01.T01 | MTR_2g022900 | 32.967 | 91 | 54 | 1 | 61 | 151 | 32 | 115 | 3.68e-12 | 60.8 |
| MsG0780038953.01.T01 | MTR_1g015165 | 32.143 | 112 | 74 | 2 | 40 | 149 | 30 | 141 | 6.62e-12 | 60.5 |
| MsG0780038953.01.T01 | MTR_3g092460 | 42.697 | 89 | 47 | 3 | 76 | 164 | 150 | 234 | 6.08e-11 | 59.3 |
| MsG0780038953.01.T01 | MTR_3g092460 | 42.697 | 89 | 47 | 3 | 76 | 164 | 181 | 265 | 7.30e-11 | 59.3 |
| MsG0780038953.01.T01 | MTR_5g061730 | 34.653 | 101 | 50 | 2 | 48 | 147 | 46 | 131 | 8.53e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038953.01.T01 | AT1G07400 | 49.180 | 122 | 60 | 1 | 46 | 165 | 35 | 156 | 5.28e-38 | 127 |
| MsG0780038953.01.T01 | AT1G59860 | 50.893 | 112 | 53 | 1 | 56 | 165 | 43 | 154 | 2.39e-35 | 121 |
| MsG0780038953.01.T01 | AT1G53540 | 39.053 | 169 | 86 | 3 | 1 | 165 | 1 | 156 | 5.52e-35 | 120 |
| MsG0780038953.01.T01 | AT2G29500 | 49.593 | 123 | 62 | 0 | 43 | 165 | 30 | 152 | 1.20e-33 | 116 |
| MsG0780038953.01.T01 | AT5G59720 | 42.262 | 168 | 84 | 4 | 1 | 165 | 1 | 158 | 4.81e-32 | 112 |
| MsG0780038953.01.T01 | AT3G46230 | 39.645 | 169 | 84 | 3 | 1 | 165 | 1 | 155 | 2.49e-29 | 105 |
| MsG0780038953.01.T01 | AT5G37670 | 42.342 | 111 | 58 | 3 | 61 | 165 | 24 | 134 | 8.07e-22 | 85.9 |
| MsG0780038953.01.T01 | AT4G10250 | 39.252 | 107 | 62 | 1 | 59 | 162 | 71 | 177 | 5.29e-20 | 82.8 |
| MsG0780038953.01.T01 | AT2G19310 | 30.128 | 156 | 82 | 6 | 1 | 151 | 1 | 134 | 3.75e-13 | 63.9 |
Find 38 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTTCCCAATAGCATCTT+TGG | 0.244934 | 7:-55717054 | MsG0780038953.01.T01:CDS |
| AAATTCCATAACCATGGTTT+TGG | 0.287141 | 7:+55716977 | None:intergenic |
| TACCTTACCTGAAAATTCTA+AGG | 0.288941 | 7:-55716686 | MsG0780038953.01.T01:CDS |
| TGATCATGTTAAGGCTTATA+TGG | 0.291004 | 7:-55716662 | MsG0780038953.01.T01:CDS |
| TCTTCGACCAAAGATGCTAT+TGG | 0.298109 | 7:+55717047 | None:intergenic |
| CATGTTTGGTGATGATCTTT+TGG | 0.315206 | 7:+55717015 | None:intergenic |
| ATCTACAAAGCACATCTTCC+CGG | 0.348790 | 7:-55716850 | MsG0780038953.01.T01:CDS |
| CTTCGACCAAAGATGCTATT+GGG | 0.363277 | 7:+55717048 | None:intergenic |
| GTTAAGGCTTATATGGATAA+TGG | 0.395831 | 7:-55716655 | MsG0780038953.01.T01:CDS |
| CTTTGTAGATATGAGCTTCA+TGG | 0.400827 | 7:+55716863 | None:intergenic |
| TTCTAAGGTTGATCATGTTA+AGG | 0.403525 | 7:-55716671 | MsG0780038953.01.T01:CDS |
| TCATACCAAAACCATGGTTA+TGG | 0.407111 | 7:-55716982 | MsG0780038953.01.T01:CDS |
| TCTATTTGAGTGTTAACAAT+TGG | 0.421750 | 7:+55716898 | None:intergenic |
| ATGATGGGTGGTGCCATGTT+TGG | 0.435221 | 7:+55717001 | None:intergenic |
| TTTGTAGATATGAGCTTCAT+GGG | 0.463063 | 7:+55716864 | None:intergenic |
| CATGTTCCTAAGCATAGAGT+TGG | 0.488524 | 7:-55716619 | MsG0780038953.01.T01:CDS |
| AGATGGAAGAAGAAAGAGGA+GGG | 0.502911 | 7:-55716753 | MsG0780038953.01.T01:CDS |
| ACATCGCTTCGTTTCAGCCC+GGG | 0.503772 | 7:+55716832 | None:intergenic |
| GTGTTCCCAACTCTATGCTT+AGG | 0.514530 | 7:+55716613 | None:intergenic |
| ATGTTCCTAAGCATAGAGTT+GGG | 0.518327 | 7:-55716618 | MsG0780038953.01.T01:CDS |
| GATGGTTCGTTGTGGAAAGG+TGG | 0.538447 | 7:+55716922 | None:intergenic |
| GTTGAGATGGAAGAAGAAAG+AGG | 0.545122 | 7:-55716757 | MsG0780038953.01.T01:CDS |
| AACATCGCTTCGTTTCAGCC+CGG | 0.548374 | 7:+55716831 | None:intergenic |
| CAAAAGATCATCACCAAACA+TGG | 0.550717 | 7:-55717014 | MsG0780038953.01.T01:CDS |
| TCTACAAAGCACATCTTCCC+GGG | 0.550939 | 7:-55716849 | MsG0780038953.01.T01:CDS |
| GGTGATGGTTCGTTGTGGAA+AGG | 0.575016 | 7:+55716919 | None:intergenic |
| TTTGAGAAATTCCATAACCA+TGG | 0.580722 | 7:+55716971 | None:intergenic |
| GGTTCGTTGTGGAAAGGTGG+TGG | 0.582470 | 7:+55716925 | None:intergenic |
| TTGTGAGAAGAATGTTGAGA+TGG | 0.597028 | 7:-55716770 | MsG0780038953.01.T01:CDS |
| TGAGTGTTAACAATTGGTGA+TGG | 0.624358 | 7:+55716904 | None:intergenic |
| TTGTTAACACTCAAATAGAG+TGG | 0.627118 | 7:-55716894 | MsG0780038953.01.T01:CDS |
| CAATTGGTGATGGTTCGTTG+TGG | 0.641191 | 7:+55716914 | None:intergenic |
| GAGATGGAAGAAGAAAGAGG+AGG | 0.653613 | 7:-55716754 | MsG0780038953.01.T01:CDS |
| CACCGTGTTGAAGTTGCTAG+TGG | 0.655046 | 7:-55716727 | MsG0780038953.01.T01:CDS |
| CACCCATCATACCAAAACCA+TGG | 0.658729 | 7:-55716988 | MsG0780038953.01.T01:CDS |
| GGTGGTGGTGTTATATGATG+TGG | 0.663623 | 7:+55716940 | None:intergenic |
| TGGAAGAAGAAAGAGGAGGG+TGG | 0.668728 | 7:-55716750 | MsG0780038953.01.T01:CDS |
| GACCACTAGCAACTTCAACA+CGG | 0.683634 | 7:+55716725 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTATTTGAGTGTTAACAAT+TGG | + | Chr7:55716744-55716763 | None:intergenic | 25.0% |
| GTTAAGGCTTATATGGATAA+TGG | - | Chr7:55716984-55717003 | MsG0780038953.01.T01:CDS | 30.0% | |
| TACCTTACCTGAAAATTCTA+AGG | - | Chr7:55716953-55716972 | MsG0780038953.01.T01:CDS | 30.0% | |
| TGATCATGTTAAGGCTTATA+TGG | - | Chr7:55716977-55716996 | MsG0780038953.01.T01:CDS | 30.0% | |
| TTGTTAACACTCAAATAGAG+TGG | - | Chr7:55716745-55716764 | MsG0780038953.01.T01:CDS | 30.0% | |
| TTTGAGAAATTCCATAACCA+TGG | + | Chr7:55716671-55716690 | None:intergenic | 30.0% | |
| TTTGTAGATATGAGCTTCAT+GGG | + | Chr7:55716778-55716797 | None:intergenic | 30.0% | |
| ! | AAATTCCATAACCATGGTTT+TGG | + | Chr7:55716665-55716684 | None:intergenic | 30.0% |
| ! | TGATCAACCTTAGAATTTTC+AGG | + | Chr7:55716963-55716982 | None:intergenic | 30.0% |
| !! | TTCTAAGGTTGATCATGTTA+AGG | - | Chr7:55716968-55716987 | MsG0780038953.01.T01:CDS | 30.0% |
| !!! | AACCTTAGAATTTTCAGGTA+AGG | + | Chr7:55716958-55716977 | None:intergenic | 30.0% |
| ATGTTCCTAAGCATAGAGTT+GGG | - | Chr7:55717021-55717040 | MsG0780038953.01.T01:CDS | 35.0% | |
| CAAAAGATCATCACCAAACA+TGG | - | Chr7:55716625-55716644 | MsG0780038953.01.T01:CDS | 35.0% | |
| CTTTGTAGATATGAGCTTCA+TGG | + | Chr7:55716779-55716798 | None:intergenic | 35.0% | |
| TCATACCAAAACCATGGTTA+TGG | - | Chr7:55716657-55716676 | MsG0780038953.01.T01:CDS | 35.0% | |
| TGAGTGTTAACAATTGGTGA+TGG | + | Chr7:55716738-55716757 | None:intergenic | 35.0% | |
| TTGTGAGAAGAATGTTGAGA+TGG | - | Chr7:55716869-55716888 | MsG0780038953.01.T01:CDS | 35.0% | |
| !!! | AACCATGGTTTTGGTATGAT+GGG | + | Chr7:55716656-55716675 | None:intergenic | 35.0% |
| !!! | CATGTTTGGTGATGATCTTT+TGG | + | Chr7:55716627-55716646 | None:intergenic | 35.0% |
| !!! | TAACCATGGTTTTGGTATGA+TGG | + | Chr7:55716657-55716676 | None:intergenic | 35.0% |
| AGATGGAAGAAGAAAGAGGA+GGG | - | Chr7:55716886-55716905 | MsG0780038953.01.T01:CDS | 40.0% | |
| ATCTACAAAGCACATCTTCC+CGG | - | Chr7:55716789-55716808 | MsG0780038953.01.T01:CDS | 40.0% | |
| CATGTTCCTAAGCATAGAGT+TGG | - | Chr7:55717020-55717039 | MsG0780038953.01.T01:CDS | 40.0% | |
| CTCTTTCCCAATAGCATCTT+TGG | - | Chr7:55716585-55716604 | MsG0780038953.01.T01:CDS | 40.0% | |
| CTTCGACCAAAGATGCTATT+GGG | + | Chr7:55716594-55716613 | None:intergenic | 40.0% | |
| GTTGAGATGGAAGAAGAAAG+AGG | - | Chr7:55716882-55716901 | MsG0780038953.01.T01:CDS | 40.0% | |
| TCTTCGACCAAAGATGCTAT+TGG | + | Chr7:55716595-55716614 | None:intergenic | 40.0% | |
| CACCCATCATACCAAAACCA+TGG | - | Chr7:55716651-55716670 | MsG0780038953.01.T01:CDS | 45.0% | |
| GACCACTAGCAACTTCAACA+CGG | + | Chr7:55716917-55716936 | None:intergenic | 45.0% | |
| GAGATGGAAGAAGAAAGAGG+AGG | - | Chr7:55716885-55716904 | MsG0780038953.01.T01:CDS | 45.0% | |
| GTGTTCCCAACTCTATGCTT+AGG | + | Chr7:55717029-55717048 | None:intergenic | 45.0% | |
| TCTACAAAGCACATCTTCCC+GGG | - | Chr7:55716790-55716809 | MsG0780038953.01.T01:CDS | 45.0% | |
| !! | CAATTGGTGATGGTTCGTTG+TGG | + | Chr7:55716728-55716747 | None:intergenic | 45.0% |
| !! | GGTGGTGGTGTTATATGATG+TGG | + | Chr7:55716702-55716721 | None:intergenic | 45.0% |
| !!! | CATGGTTTTGGTATGATGGG+TGG | + | Chr7:55716653-55716672 | None:intergenic | 45.0% |
| AACATCGCTTCGTTTCAGCC+CGG | + | Chr7:55716811-55716830 | None:intergenic | 50.0% | |
| CACCGTGTTGAAGTTGCTAG+TGG | - | Chr7:55716912-55716931 | MsG0780038953.01.T01:CDS | 50.0% | |
| GATGGTTCGTTGTGGAAAGG+TGG | + | Chr7:55716720-55716739 | None:intergenic | 50.0% | |
| TGGAAGAAGAAAGAGGAGGG+TGG | - | Chr7:55716889-55716908 | MsG0780038953.01.T01:CDS | 50.0% | |
| ! | GGTGATGGTTCGTTGTGGAA+AGG | + | Chr7:55716723-55716742 | None:intergenic | 50.0% |
| !! | ATGATGGGTGGTGCCATGTT+TGG | + | Chr7:55716641-55716660 | None:intergenic | 50.0% |
| ACATCGCTTCGTTTCAGCCC+GGG | + | Chr7:55716810-55716829 | None:intergenic | 55.0% | |
| GGTTCGTTGTGGAAAGGTGG+TGG | + | Chr7:55716717-55716736 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 55716579 | 55717082 | 55716579 | ID=MsG0780038953.01;Name=MsG0780038953.01 |
| Chr7 | mRNA | 55716579 | 55717082 | 55716579 | ID=MsG0780038953.01.T01;Parent=MsG0780038953.01;Name=MsG0780038953.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|167 |
| Chr7 | exon | 55716579 | 55717082 | 55716579 | ID=MsG0780038953.01.T01:exon:6254;Parent=MsG0780038953.01.T01 |
| Chr7 | CDS | 55716579 | 55717082 | 55716579 | ID=MsG0780038953.01.T01:cds;Parent=MsG0780038953.01.T01 |
| Gene Sequence |
| Protein sequence |
