AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039412.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039412.01.T01 MTR_0121s0080 87.387 222 28 0 1 222 15 236 7.64e-143 398
MsG0780039412.01.T01 MTR_7g062350 86.854 213 28 0 10 222 36 248 9.30e-138 386
MsG0780039412.01.T01 MTR_8g043650 72.197 223 61 1 1 222 15 237 5.52e-115 328
MsG0780039412.01.T01 MTR_1g105920 74.146 205 53 0 1 205 15 219 9.06e-109 312
MsG0780039412.01.T01 MTR_1g105910 72.038 211 59 0 1 211 15 225 5.76e-108 310
MsG0780039412.01.T01 MTR_2g030740 73.659 205 54 0 1 205 15 219 1.49e-107 309
MsG0780039412.01.T01 MTR_1g012570 71.220 205 59 0 1 205 15 219 2.44e-104 301
MsG0780039412.01.T01 MTR_1g108510 68.778 221 51 3 1 211 15 227 1.31e-103 299
MsG0780039412.01.T01 MTR_2g093190 69.683 221 49 3 1 211 15 227 1.54e-102 296
MsG0780039412.01.T01 MTR_3g030770 68.778 221 51 3 1 211 15 227 4.66e-102 295
MsG0780039412.01.T01 MTR_1g105905 65.909 220 74 1 4 222 1 220 7.22e-102 294
MsG0780039412.01.T01 MTR_3g052920 69.268 205 63 0 1 205 15 219 6.15e-101 292
MsG0780039412.01.T01 MTR_1g108580 68.018 222 51 3 1 211 15 227 4.10e-100 290
MsG0780039412.01.T01 MTR_2g085280 63.063 222 81 1 1 221 15 236 7.24e-98 285
MsG0780039412.01.T01 MTR_2g085250 63.063 222 81 1 1 221 15 236 2.63e-97 283
MsG0780039412.01.T01 MTR_1g075600 63.033 211 78 0 1 211 15 225 4.00e-94 275
MsG0780039412.01.T01 MTR_5g055100 60.090 223 88 1 1 222 15 237 2.25e-93 273
MsG0780039412.01.T01 MTR_1g077360 61.611 211 81 0 1 211 15 225 1.46e-92 271
MsG0780039412.01.T01 MTR_5g053390 62.559 211 79 0 1 211 15 225 2.79e-89 263
MsG0780039412.01.T01 MTR_5g045560 61.137 211 82 0 1 211 15 225 5.14e-89 262
MsG0780039412.01.T01 MTR_3g052870 63.415 205 56 1 1 205 15 200 1.02e-85 253
MsG0780039412.01.T01 MTR_1g106070 72.455 167 46 0 1 167 15 181 7.89e-85 249
MsG0780039412.01.T01 MTR_3g030780 83.898 118 19 0 1 118 15 132 8.41e-67 202
MsG0780039412.01.T01 MTR_0121s0100 73.729 118 31 0 1 118 15 132 1.60e-55 173
MsG0780039412.01.T01 MTR_1g075570 55.618 178 65 1 4 181 1 164 1.18e-54 172
MsG0780039412.01.T01 MTR_3g080940 53.425 146 68 0 1 146 15 160 3.80e-52 167
MsG0780039412.01.T01 MTR_3g067910 63.725 102 36 1 7 107 2 103 7.18e-40 133
MsG0780039412.01.T01 MTR_1g108500 72.165 97 6 1 8 104 110 185 9.69e-37 131
MsG0780039412.01.T01 MTR_8g086290 37.791 172 91 3 1 169 18 176 9.18e-31 112
MsG0780039412.01.T01 MTR_2g105290 36.601 153 93 1 1 153 18 166 7.78e-29 107
MsG0780039412.01.T01 MTR_7g028448 44.203 138 65 3 8 145 25 150 6.52e-28 104
MsG0780039412.01.T01 MTR_4g036915 44.203 138 65 3 8 145 25 150 6.52e-28 104
MsG0780039412.01.T01 MTR_3g067875 80.357 56 11 0 4 59 1 56 5.03e-26 96.3
MsG0780039412.01.T01 MTR_3g067915 65.672 67 23 0 46 112 30 96 1.78e-23 90.5
MsG0780039412.01.T01 MTR_4g127140 32.927 164 96 3 8 161 25 184 2.76e-23 92.8
MsG0780039412.01.T01 MTR_1g054265 55.556 72 32 0 1 72 8 79 1.75e-20 85.1
MsG0780039412.01.T01 MTR_3g067870 63.492 63 23 0 2 64 83 145 2.12e-20 84.3
MsG0780039412.01.T01 MTR_1g047550 55.405 74 24 1 11 75 18 91 4.57e-20 84.3
MsG0780039412.01.T01 MTR_4g131030 41.284 109 49 2 11 104 18 126 7.53e-20 84.3
MsG0780039412.01.T01 MTR_8g079502 67.925 53 17 0 11 63 18 70 1.95e-19 82.4
MsG0780039412.01.T01 MTR_8g051580 66.038 53 18 0 11 63 18 70 2.33e-19 82.4
MsG0780039412.01.T01 MTR_1g063160 44.211 95 51 1 1 95 8 100 2.69e-19 82.0
MsG0780039412.01.T01 MTR_8g046350 62.264 53 20 0 11 63 18 70 6.92e-18 78.2
MsG0780039412.01.T01 MTR_4g102530 47.059 68 36 0 1 68 8 75 6.20e-16 73.9
MsG0780039412.01.T01 MTR_4g094632 38.614 101 60 1 4 104 11 109 1.23e-15 72.0
MsG0780039412.01.T01 MTR_4g093970 32.000 200 100 6 1 172 34 225 1.82e-15 73.2
MsG0780039412.01.T01 MTR_3g102570 43.750 96 51 3 1 95 8 101 5.00e-15 72.8
MsG0780039412.01.T01 MTR_8g033220 44.304 79 43 1 1 78 8 86 1.41e-14 70.5
MsG0780039412.01.T01 MTR_8g033220 44.304 79 43 1 1 78 8 86 1.82e-14 70.1
MsG0780039412.01.T01 MTR_3g005530 46.774 62 33 0 1 62 8 69 1.90e-14 70.1
MsG0780039412.01.T01 MTR_7g075870 48.387 62 32 0 1 62 8 69 6.51e-14 68.6
MsG0780039412.01.T01 MTR_4g094638 37.000 100 62 1 1 99 8 107 6.85e-14 67.8
MsG0780039412.01.T01 MTR_8g022970 33.121 157 87 5 13 163 36 180 7.48e-14 67.8
MsG0780039412.01.T01 MTR_8g033270 48.387 62 32 0 1 62 8 69 9.52e-14 68.6
MsG0780039412.01.T01 MTR_5g066180 46.774 62 33 0 1 62 8 69 1.07e-13 68.2
MsG0780039412.01.T01 MTR_6g464720 47.619 63 32 1 1 62 8 70 1.21e-13 68.2
MsG0780039412.01.T01 MTR_3g452380 41.667 72 42 0 1 72 23 94 1.56e-13 67.8
MsG0780039412.01.T01 MTR_1g101970 46.774 62 33 0 1 62 8 69 1.85e-13 66.6
MsG0780039412.01.T01 MTR_4g084740 47.143 70 35 2 1 69 8 76 3.13e-13 68.2
MsG0780039412.01.T01 MTR_3g437790 34.266 143 77 4 12 145 30 164 3.21e-13 65.9
MsG0780039412.01.T01 MTR_4g109830 46.032 63 33 1 1 62 8 70 3.78e-13 66.6
MsG0780039412.01.T01 MTR_5g032520 45.161 62 34 0 1 62 8 69 4.07e-13 66.6
MsG0780039412.01.T01 MTR_2g009890 43.548 62 35 0 1 62 8 69 4.14e-13 67.0
MsG0780039412.01.T01 MTR_5g032150 45.161 62 34 0 1 62 8 69 4.94e-13 66.2
MsG0780039412.01.T01 MTR_7g016630 35.455 110 60 4 1 100 8 116 5.53e-13 66.6
MsG0780039412.01.T01 MTR_8g087860 36.957 92 51 2 1 86 24 114 6.66e-13 66.2
MsG0780039412.01.T01 MTR_7g075870 47.619 63 32 1 1 62 8 70 8.36e-13 65.5
MsG0780039412.01.T01 MTR_1g053070 36.842 95 59 1 1 94 8 102 8.86e-13 65.9
MsG0780039412.01.T01 MTR_5g031000 43.548 62 35 0 1 62 8 69 1.12e-12 65.1
MsG0780039412.01.T01 MTR_3g109930 32.847 137 82 2 13 145 20 150 1.16e-12 64.3
MsG0780039412.01.T01 MTR_5g031000 43.548 62 35 0 1 62 8 69 1.35e-12 65.1
MsG0780039412.01.T01 MTR_0003s0590 38.158 76 47 0 1 76 8 83 1.38e-12 65.5
MsG0780039412.01.T01 MTR_2g461710 54.000 50 23 0 1 50 8 57 2.14e-12 60.5
MsG0780039412.01.T01 MTR_2g017865 45.161 62 34 0 1 62 23 84 2.62e-12 64.7
MsG0780039412.01.T01 MTR_8g066260 44.444 63 34 1 1 62 8 70 2.91e-12 64.3
MsG0780039412.01.T01 MTR_6g015975 46.032 63 33 1 1 62 8 70 7.75e-12 63.2
MsG0780039412.01.T01 MTR_7g016600 46.032 63 33 1 1 62 8 70 8.72e-12 63.2
MsG0780039412.01.T01 MTR_8g097090 37.234 94 55 2 1 90 8 101 1.48e-11 62.4
MsG0780039412.01.T01 MTR_3g084980 35.294 102 62 2 1 98 8 109 2.16e-11 62.0
MsG0780039412.01.T01 MTR_3g113030 45.614 57 31 0 1 57 8 64 2.33e-11 61.6
MsG0780039412.01.T01 MTR_5g046790 35.780 109 51 5 1 97 8 109 3.78e-11 60.8
MsG0780039412.01.T01 MTR_4g109810 27.660 235 125 12 1 211 8 221 6.68e-11 60.5
MsG0780039412.01.T01 MTR_5g046870 42.857 63 35 1 1 62 8 70 8.01e-11 57.8
MsG0780039412.01.T01 MTR_5g021270 43.750 64 34 1 1 62 8 71 9.48e-11 60.1
MsG0780039412.01.T01 MTR_5g021270 43.750 64 34 1 1 62 8 71 9.97e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039412.01.T01 AT5G60440 53.503 157 70 2 1 154 13 169 1.52e-48 161
MsG0780039412.01.T01 AT2G24840 44.156 154 77 3 1 149 69 218 1.59e-34 124
MsG0780039412.01.T01 AT1G01530 43.046 151 83 2 3 152 15 163 1.17e-31 116
MsG0780039412.01.T01 AT1G65360 35.211 213 114 7 3 197 15 221 2.71e-31 114
MsG0780039412.01.T01 AT4G36590 37.209 172 100 3 1 165 13 183 2.51e-27 105
MsG0780039412.01.T01 AT2G34440 42.268 97 55 1 11 107 18 113 4.29e-19 81.6
MsG0780039412.01.T01 AT3G66656 38.053 113 66 1 1 109 8 120 2.10e-18 79.7
MsG0780039412.01.T01 AT3G04100 30.519 154 93 4 1 145 20 168 7.48e-18 79.0
MsG0780039412.01.T01 AT2G45660 33.846 130 61 2 1 105 8 137 5.19e-17 75.5
MsG0780039412.01.T01 AT2G45660 33.846 130 61 2 1 105 8 137 1.82e-16 75.5
MsG0780039412.01.T01 AT1G28460 31.176 170 103 4 1 163 14 176 3.02e-16 74.3
MsG0780039412.01.T01 AT4G22950 46.479 71 38 0 1 71 8 78 5.41e-16 73.2
MsG0780039412.01.T01 AT4G09960 37.607 117 63 3 1 112 8 119 1.16e-15 73.2
MsG0780039412.01.T01 AT4G09960 37.607 117 63 3 1 112 9 120 1.49e-15 73.2
MsG0780039412.01.T01 AT4G09960 37.607 117 63 3 1 112 8 119 1.53e-15 73.2
MsG0780039412.01.T01 AT4G09960 37.607 117 63 3 1 112 18 129 1.70e-15 73.2
MsG0780039412.01.T01 AT1G72350 49.254 67 32 1 1 67 49 113 1.99e-15 72.8
MsG0780039412.01.T01 AT4G09960 37.607 117 63 3 1 112 34 145 2.16e-15 73.2
MsG0780039412.01.T01 AT4G22950 45.833 72 39 0 1 72 8 79 2.93e-15 72.4
MsG0780039412.01.T01 AT4G22950 45.833 72 39 0 1 72 8 79 2.93e-15 72.4
MsG0780039412.01.T01 AT4G09960 37.607 117 63 3 1 112 103 214 4.43e-15 73.2
MsG0780039412.01.T01 AT2G45650 50.000 62 31 0 1 62 8 69 6.33e-15 72.0
MsG0780039412.01.T01 AT4G18960 37.234 94 49 1 1 94 24 107 1.43e-14 70.9
MsG0780039412.01.T01 AT5G51870 50.000 62 31 0 1 62 8 69 1.96e-14 68.9
MsG0780039412.01.T01 AT5G51870 50.000 62 31 0 1 62 8 69 2.12e-14 69.7
MsG0780039412.01.T01 AT5G51870 50.000 62 31 0 1 62 8 69 3.62e-14 69.3
MsG0780039412.01.T01 AT5G51870 50.000 62 31 0 1 62 18 79 3.98e-14 69.3
MsG0780039412.01.T01 AT5G51870 50.000 62 31 0 1 62 31 92 4.81e-14 68.6
MsG0780039412.01.T01 AT4G24540 48.387 62 32 0 1 62 8 69 5.57e-14 68.9
MsG0780039412.01.T01 AT2G22540 48.387 62 32 0 1 62 8 69 5.83e-14 68.9
MsG0780039412.01.T01 AT2G22540 48.387 62 32 0 1 62 8 69 5.83e-14 68.9
MsG0780039412.01.T01 AT3G57230 41.935 62 36 0 1 62 8 69 6.54e-14 67.0
MsG0780039412.01.T01 AT1G22130 37.931 87 53 1 1 86 8 94 7.87e-14 69.7
MsG0780039412.01.T01 AT1G77980 47.692 65 33 1 1 64 8 72 1.03e-13 69.3
MsG0780039412.01.T01 AT4G37940 43.548 62 35 0 1 62 8 69 1.25e-13 68.2
MsG0780039412.01.T01 AT4G37940 43.548 62 35 0 1 62 8 69 1.32e-13 67.0
MsG0780039412.01.T01 AT4G37940 43.548 62 35 0 1 62 8 69 1.36e-13 67.8
MsG0780039412.01.T01 AT3G54340 34.746 118 63 4 1 111 8 118 1.41e-13 67.8
MsG0780039412.01.T01 AT1G77950 44.286 70 37 2 1 69 8 76 1.80e-13 67.8
MsG0780039412.01.T01 AT1G77950 44.286 70 37 2 1 69 8 76 1.80e-13 67.8
MsG0780039412.01.T01 AT1G77950 44.286 70 37 2 1 69 8 76 1.80e-13 67.8
MsG0780039412.01.T01 AT1G77950 44.286 70 37 2 1 69 8 76 1.80e-13 67.8
MsG0780039412.01.T01 AT1G29962 29.885 174 103 6 1 163 14 179 2.01e-13 66.6
MsG0780039412.01.T01 AT1G17310 31.788 151 62 4 11 156 64 178 2.82e-13 67.0
MsG0780039412.01.T01 AT3G57230 41.935 62 36 0 1 62 8 69 3.37e-13 67.0
MsG0780039412.01.T01 AT3G57230 41.935 62 36 0 1 62 8 69 3.37e-13 67.0
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 3.53e-13 65.5
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 3.53e-13 65.5
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 3.53e-13 65.5
MsG0780039412.01.T01 AT1G17310 31.788 151 62 4 11 156 75 189 3.53e-13 66.6
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 3.56e-13 63.9
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 4.81e-13 64.7
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 4.81e-13 64.7
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 4.81e-13 64.7
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 4.81e-13 64.7
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 4.81e-13 64.7
MsG0780039412.01.T01 AT5G51860 46.774 62 33 0 1 62 8 69 5.21e-13 65.9
MsG0780039412.01.T01 AT5G51860 46.774 62 33 0 1 62 8 69 6.29e-13 65.9
MsG0780039412.01.T01 AT5G13790 46.774 62 33 0 1 62 8 69 8.61e-13 66.2
MsG0780039412.01.T01 AT5G13790 46.774 62 33 0 1 62 8 69 8.62e-13 66.2
MsG0780039412.01.T01 AT4G11880 44.776 67 36 1 1 66 8 74 1.19e-12 64.3
MsG0780039412.01.T01 AT4G11880 44.776 67 36 1 1 66 8 74 1.19e-12 64.3
MsG0780039412.01.T01 AT2G42830 45.161 62 34 0 1 62 23 84 1.22e-12 65.5
MsG0780039412.01.T01 AT2G42830 45.161 62 34 0 1 62 23 84 1.25e-12 65.5
MsG0780039412.01.T01 AT3G57390 45.161 62 34 0 1 62 8 69 1.28e-12 65.1
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 1.40e-12 64.7
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 1.40e-12 64.7
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 1.40e-12 64.7
MsG0780039412.01.T01 AT2G22630 41.935 62 36 0 1 62 8 69 1.42e-12 65.1
MsG0780039412.01.T01 AT2G22630 41.935 62 36 0 1 62 8 69 1.42e-12 65.1
MsG0780039412.01.T01 AT3G30260 44.444 63 34 1 1 62 8 70 1.45e-12 65.5
MsG0780039412.01.T01 AT3G58780 45.161 62 34 0 1 62 23 84 1.49e-12 65.5
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 1.50e-12 64.3
MsG0780039412.01.T01 AT5G62165 43.548 62 35 0 1 62 8 69 1.50e-12 64.3
MsG0780039412.01.T01 AT3G58780 45.161 62 34 0 1 62 29 90 1.69e-12 65.1
MsG0780039412.01.T01 AT1G69120 46.032 63 33 1 1 62 8 70 1.76e-12 65.1
MsG0780039412.01.T01 AT4G11880 44.776 67 36 1 1 66 8 74 2.19e-12 64.3
MsG0780039412.01.T01 AT4G11880 44.776 67 36 1 1 66 8 74 2.19e-12 64.3
MsG0780039412.01.T01 AT5G60910 46.032 63 33 1 1 62 8 70 2.20e-12 64.7
MsG0780039412.01.T01 AT5G10140 47.619 63 32 1 1 62 8 70 2.21e-12 63.5
MsG0780039412.01.T01 AT3G57390 45.161 62 34 0 1 62 8 69 2.41e-12 64.7
MsG0780039412.01.T01 AT3G58780 45.161 62 34 0 1 62 23 84 2.90e-12 64.7
MsG0780039412.01.T01 AT5G10140 47.619 63 32 1 1 62 8 70 3.39e-12 63.2
MsG0780039412.01.T01 AT1G69120 46.032 63 33 1 1 62 8 70 3.54e-12 64.3
MsG0780039412.01.T01 AT5G10140 47.619 63 32 1 1 62 8 70 3.55e-12 63.5
MsG0780039412.01.T01 AT5G10140 47.619 63 32 1 1 62 8 70 3.70e-12 63.2
MsG0780039412.01.T01 AT2G14210 41.270 63 36 1 1 62 8 70 4.64e-12 63.5
MsG0780039412.01.T01 AT1G28450 29.586 169 99 6 1 154 14 177 5.98e-12 62.8
MsG0780039412.01.T01 AT2G14210 41.270 63 36 1 1 62 8 70 6.26e-12 63.5
MsG0780039412.01.T01 AT1G26310 44.444 63 34 1 1 62 8 70 6.73e-12 63.5
MsG0780039412.01.T01 AT3G61120 45.161 62 34 0 1 62 8 69 7.33e-12 63.2
MsG0780039412.01.T01 AT2G22540 46.774 62 28 1 1 62 8 64 1.03e-11 62.8
MsG0780039412.01.T01 AT5G15800 46.032 63 33 1 1 62 8 70 1.80e-11 62.4
MsG0780039412.01.T01 AT1G47760 38.824 85 38 2 11 95 18 88 1.98e-11 61.2
MsG0780039412.01.T01 AT5G15800 46.032 63 33 1 1 62 8 70 2.83e-11 62.0

Find 49 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTATTCAAGAAGATTT+TGG 0.235888 7:+63700245 MsG0780039412.01.T01:CDS
ATCTCAGTTTGTTGAGGATT+TGG 0.255732 7:-63700390 None:intergenic
ATGTTGCAACCTCATTTAAT+TGG 0.256996 7:+63700441 MsG0780039412.01.T01:CDS
TGTTGATAGGAGTGCAATTC+AGG 0.259070 7:+63700296 MsG0780039412.01.T01:CDS
TTTAATTGGCTTCAACAATA+TGG 0.267629 7:+63700455 MsG0780039412.01.T01:CDS
AAATCATTACCAAGAGAAAA+TGG 0.314064 7:-63700339 None:intergenic
AATATGGAAGGAGGAGGATA+TGG 0.330377 7:+63700471 MsG0780039412.01.T01:CDS
AGGCGTTCAAGGCTCAATTT+TGG 0.334747 7:+63700184 MsG0780039412.01.T01:CDS
GCCCTATTCATTCTATCAAT+TGG 0.337270 7:-63700216 None:intergenic
GTCATAGATCGCTATCTCTC+TGG 0.357204 7:+63700015 MsG0780039412.01.T01:CDS
CCTTGAACGCCTTGCACAAA+AGG 0.373825 7:-63700173 None:intergenic
GTTGAGGATTTGGCTGATAA+TGG 0.382243 7:-63700380 None:intergenic
TCCAATTGATAGAATGAATA+GGG 0.395255 7:+63700215 MsG0780039412.01.T01:CDS
AATGAGCTTTGCACCCTTTG+TGG 0.404337 7:+63699919 MsG0780039412.01.T01:CDS
TTGTTGCTCAACATGTTGAT+AGG 0.407276 7:+63700283 MsG0780039412.01.T01:CDS
CTAGAAACTTCAATAGAATC+CGG 0.407513 7:-63700499 None:intergenic
ATTAAATGAGGTTGCAACAT+AGG 0.428043 7:-63700438 None:intergenic
GTCCAATTGATAGAATGAAT+AGG 0.444780 7:+63700214 MsG0780039412.01.T01:CDS
TCTATGACCGTATCAACATT+TGG 0.448129 7:-63700000 None:intergenic
ATAATGGAGAGGGATGTTAG+AGG 0.448969 7:-63700364 None:intergenic
TTAAATGAGGTTGCAACATA+GGG 0.493554 7:-63700437 None:intergenic
AGCATTGAGCTCACGCACTT+TGG 0.503646 7:-63700091 None:intergenic
AGCAACATCTACACCACAAA+GGG 0.507315 7:-63699932 None:intergenic
CGGTCATCCAAATGTTGATA+CGG 0.514501 7:+63699993 MsG0780039412.01.T01:CDS
TAGAAACTTCAATAGAATCC+GGG 0.517746 7:-63700498 None:intergenic
TGTATTCTCACCTAGTGAAA+AGG 0.518205 7:+63699960 MsG0780039412.01.T01:CDS
TCATAGATCGCTATCTCTCT+GGG 0.520984 7:+63700016 MsG0780039412.01.T01:CDS
GTTGATAGGAGTGCAATTCA+GGG 0.537321 7:+63700297 MsG0780039412.01.T01:CDS
ATTGGCTTCAACAATATGGA+AGG 0.538957 7:+63700459 MsG0780039412.01.T01:CDS
GCTTTGAGAAAGTCACTTGG+AGG 0.544594 7:-63699870 None:intergenic
GCTCACGCACTTTGGCGCTA+CGG 0.549758 7:-63700083 None:intergenic
TTGTTGAAGCCAATTAAATG+AGG 0.551491 7:-63700450 None:intergenic
GAAAACATCTCAGTTTGTTG+AGG 0.554882 7:-63700396 None:intergenic
CTTTCTCAAAGCGTCGTAGT+GGG 0.584950 7:+63699881 MsG0780039412.01.T01:CDS
AATCAACAACACACTGGACG+AGG 0.587728 7:+63700125 MsG0780039412.01.T01:CDS
TTCAACAATATGGAAGGAGG+AGG 0.598809 7:+63700465 MsG0780039412.01.T01:CDS
ACTTTCTCAAAGCGTCGTAG+TGG 0.602122 7:+63699880 MsG0780039412.01.T01:CDS
GTGAGCCTCAATGAATTCCA+TGG 0.607397 7:-63700061 None:intergenic
GACGCTTTGAGAAAGTCACT+TGG 0.608413 7:-63699873 None:intergenic
AGAAACTTCAATAGAATCCG+GGG 0.619380 7:-63700497 None:intergenic
GGATTTGGCTGATAATGGAG+AGG 0.633381 7:-63700375 None:intergenic
GGCTTCAACAATATGGAAGG+AGG 0.642874 7:+63700462 MsG0780039412.01.T01:CDS
GTTTCAAATCAACAACACAC+TGG 0.650841 7:+63700119 MsG0780039412.01.T01:CDS
TAGCACCATGGAATTCATTG+AGG 0.666363 7:+63700056 MsG0780039412.01.T01:CDS
GAGCAACATCTACACCACAA+AGG 0.666690 7:-63699933 None:intergenic
GAAACTTCAATAGAATCCGG+GGG 0.679421 7:-63700496 None:intergenic
GATTTGGCTGATAATGGAGA+GGG 0.685158 7:-63700374 None:intergenic
ACCGCAAAACAATAGCACCA+TGG 0.712731 7:+63700044 MsG0780039412.01.T01:CDS
AAACTTCAATAGAATCCGGG+GGG 0.771286 7:-63700495 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTATTCAAGAAGATTT+TGG + Chr7:63700245-63700264 MsG0780039412.01.T01:CDS 20.0%
! AAATCATTACCAAGAGAAAA+TGG - Chr7:63700342-63700361 None:intergenic 25.0%
! ATAGGGTTATGAAAAATTTG+TGG - Chr7:63700423-63700442 None:intergenic 25.0%
! TCCAATTGATAGAATGAATA+GGG + Chr7:63700215-63700234 MsG0780039412.01.T01:CDS 25.0%
!! AAAGAAAATACCTTTTCACT+AGG - Chr7:63699973-63699992 None:intergenic 25.0%
!! ATTATTCAAGAAGATTTTGG+TGG + Chr7:63700248-63700267 MsG0780039412.01.T01:CDS 25.0%
!! TTTAATTGGCTTCAACAATA+TGG + Chr7:63700455-63700474 MsG0780039412.01.T01:CDS 25.0%
!!! AGTGAAAAGGTATTTTCTTT+CGG + Chr7:63699973-63699992 MsG0780039412.01.T01:CDS 25.0%
ATGTTGCAACCTCATTTAAT+TGG + Chr7:63700441-63700460 MsG0780039412.01.T01:CDS 30.0%
ATTAAATGAGGTTGCAACAT+AGG - Chr7:63700441-63700460 None:intergenic 30.0%
GTCCAATTGATAGAATGAAT+AGG + Chr7:63700214-63700233 MsG0780039412.01.T01:CDS 30.0%
TTAAATGAGGTTGCAACATA+GGG - Chr7:63700440-63700459 None:intergenic 30.0%
TTGTTGAAGCCAATTAAATG+AGG - Chr7:63700453-63700472 None:intergenic 30.0%
GCCCTATTCATTCTATCAAT+TGG - Chr7:63700219-63700238 None:intergenic 35.0%
GTTTCAAATCAACAACACAC+TGG + Chr7:63700119-63700138 MsG0780039412.01.T01:CDS 35.0%
TCTATGACCGTATCAACATT+TGG - Chr7:63700003-63700022 None:intergenic 35.0%
TGTATTCTCACCTAGTGAAA+AGG + Chr7:63699960-63699979 MsG0780039412.01.T01:CDS 35.0%
TTGTTGCTCAACATGTTGAT+AGG + Chr7:63700283-63700302 MsG0780039412.01.T01:CDS 35.0%
! ATTGGCTTCAACAATATGGA+AGG + Chr7:63700459-63700478 MsG0780039412.01.T01:CDS 35.0%
! CACACTTTTCCATTTTCTCT+TGG + Chr7:63700330-63700349 MsG0780039412.01.T01:CDS 35.0%
!! ATCTCAGTTTGTTGAGGATT+TGG - Chr7:63700393-63700412 None:intergenic 35.0%
!! GAAAACATCTCAGTTTGTTG+AGG - Chr7:63700399-63700418 None:intergenic 35.0%
AATATGGAAGGAGGAGGATA+TGG + Chr7:63700471-63700490 MsG0780039412.01.T01:CDS 40.0%
AGCAACATCTACACCACAAA+GGG - Chr7:63699935-63699954 None:intergenic 40.0%
CGGTCATCCAAATGTTGATA+CGG + Chr7:63699993-63700012 MsG0780039412.01.T01:CDS 40.0%
GTTGAGGATTTGGCTGATAA+TGG - Chr7:63700383-63700402 None:intergenic 40.0%
TAGCACCATGGAATTCATTG+AGG + Chr7:63700056-63700075 MsG0780039412.01.T01:CDS 40.0%
TCATAGATCGCTATCTCTCT+GGG + Chr7:63700016-63700035 MsG0780039412.01.T01:CDS 40.0%
TTCAACAATATGGAAGGAGG+AGG + Chr7:63700465-63700484 MsG0780039412.01.T01:CDS 40.0%
! ATAATGGAGAGGGATGTTAG+AGG - Chr7:63700367-63700386 None:intergenic 40.0%
! GTTGATAGGAGTGCAATTCA+GGG + Chr7:63700297-63700316 MsG0780039412.01.T01:CDS 40.0%
! TGTTGATAGGAGTGCAATTC+AGG + Chr7:63700296-63700315 MsG0780039412.01.T01:CDS 40.0%
!! GATTTGGCTGATAATGGAGA+GGG - Chr7:63700377-63700396 None:intergenic 40.0%
!! GTTCAAGGCTCAATTTTGGT+GGG + Chr7:63700188-63700207 MsG0780039412.01.T01:CDS 40.0%
!!! TCCATGGTGCTATTGTTTTG+CGG - Chr7:63700048-63700067 None:intergenic 40.0%
AATCAACAACACACTGGACG+AGG + Chr7:63700125-63700144 MsG0780039412.01.T01:CDS 45.0%
AATGAGCTTTGCACCCTTTG+TGG + Chr7:63699919-63699938 MsG0780039412.01.T01:CDS 45.0%
ACCGCAAAACAATAGCACCA+TGG + Chr7:63700044-63700063 MsG0780039412.01.T01:CDS 45.0%
GAGCAACATCTACACCACAA+AGG - Chr7:63699936-63699955 None:intergenic 45.0%
GGCTTCAACAATATGGAAGG+AGG + Chr7:63700462-63700481 MsG0780039412.01.T01:CDS 45.0%
GTCATAGATCGCTATCTCTC+TGG + Chr7:63700015-63700034 MsG0780039412.01.T01:CDS 45.0%
GTGAGCCTCAATGAATTCCA+TGG - Chr7:63700064-63700083 None:intergenic 45.0%
! AGGCGTTCAAGGCTCAATTT+TGG + Chr7:63700184-63700203 MsG0780039412.01.T01:CDS 45.0%
! CGTTCAAGGCTCAATTTTGG+TGG + Chr7:63700187-63700206 MsG0780039412.01.T01:CDS 45.0%
!! ACTTTCTCAAAGCGTCGTAG+TGG + Chr7:63699880-63699899 MsG0780039412.01.T01:CDS 45.0%
!! CTTTCTCAAAGCGTCGTAGT+GGG + Chr7:63699881-63699900 MsG0780039412.01.T01:CDS 45.0%
!! GACGCTTTGAGAAAGTCACT+TGG - Chr7:63699876-63699895 None:intergenic 45.0%
!! GCTTTGAGAAAGTCACTTGG+AGG - Chr7:63699873-63699892 None:intergenic 45.0%
!! GGATTTGGCTGATAATGGAG+AGG - Chr7:63700378-63700397 None:intergenic 45.0%
!! TGAGTTGAGCCTTTTGTGCA+AGG + Chr7:63700164-63700183 MsG0780039412.01.T01:CDS 45.0%
!!! ATGGTGCTATTGTTTTGCGG+TGG - Chr7:63700045-63700064 None:intergenic 45.0%
!!! TGGTGCTATTGTTTTGCGGT+GGG - Chr7:63700044-63700063 None:intergenic 45.0%
CCTTGAACGCCTTGCACAAA+AGG - Chr7:63700176-63700195 None:intergenic 50.0%
CTGGACGAGGAGAAAAAGTG+TGG + Chr7:63700138-63700157 MsG0780039412.01.T01:CDS 50.0%
! AGCATTGAGCTCACGCACTT+TGG - Chr7:63700094-63700113 None:intergenic 50.0%
!! CCTTTTGTGCAAGGCGTTCA+AGG + Chr7:63700173-63700192 MsG0780039412.01.T01:CDS 50.0%
GCTCACGCACTTTGGCGCTA+CGG - Chr7:63700086-63700105 None:intergenic 60.0%
GGAGGAGGATATGGACCCCC+CGG + Chr7:63700480-63700499 MsG0780039412.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 63699844 63700512 63699844 ID=MsG0780039412.01;Name=MsG0780039412.01
Chr7 mRNA 63699844 63700512 63699844 ID=MsG0780039412.01.T01;Parent=MsG0780039412.01;Name=MsG0780039412.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|222
Chr7 exon 63699844 63700512 63699844 ID=MsG0780039412.01.T01:exon:15937;Parent=MsG0780039412.01.T01
Chr7 CDS 63699844 63700512 63699844 ID=MsG0780039412.01.T01:cds;Parent=MsG0780039412.01.T01
Gene Sequence

>MsG0780039412.01.T01

ATGAAAAAGATGAGCAACGAGAGCAACCTCCAAGTGACTTTCTCAAAGCGTCGTAGTGGGCTCTTCAAGAAAGTCAATGAGCTTTGCACCCTTTGTGGTGTAGATGTTGCTCTCGTTGTATTCTCACCTAGTGAAAAGGTATTTTCTTTCGGTCATCCAAATGTTGATACGGTCATAGATCGCTATCTCTCTGGGATCCCACCGCAAAACAATAGCACCATGGAATTCATTGAGGCTCACCGTAGCGCCAAAGTGCGTGAGCTCAATGCTGAGTTGTTTCAAATCAACAACACACTGGACGAGGAGAAAAAGTGTGGTGATGAGTTGAGCCTTTTGTGCAAGGCGTTCAAGGCTCAATTTTGGTGGGCTTGTCCAATTGATAGAATGAATAGGGCTCAGCTTGAATTATTCAAGAAGATTTTGGTGGAGCTTAAGAAACTTGTTGCTCAACATGTTGATAGGAGTGCAATTCAGGGTGCTTCTGCTCACACTTTTCCATTTTCTCTTGGTAATGATTTATCCTCTAACATCCCTCTCCATTATCAGCCAAATCCTCAACAAACTGAGATGTTTTCGCCACAAATTTTTCATAACCCTATGTTGCAACCTCATTTAATTGGCTTCAACAATATGGAAGGAGGAGGATATGGACCCCCCGGATTCTATTGA
Protein sequence

>MsG0780039412.01.T01

MKKMSNESNLQVTFSKRRSGLFKKVNELCTLCGVDVALVVFSPSEKVFSFGHPNVDTVIDRYLSGIPPQNNSTMEFIEAHRSAKVRELNAELFQINNTLDEEKKCGDELSLLCKAFKAQFWWACPIDRMNRAQLELFKKILVELKKLVAQHVDRSAIQGASAHTFPFSLGNDLSSNIPLHYQPNPQQTEMFSPQIFHNPMLQPHLIGFNNMEGGGYGPPGFY*