AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039572.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039572.01.T01 MTR_7g074880 95.671 231 10 0 2 232 96 326 2.19e-166 462
MsG0780039572.01.T01 MTR_7g074870 89.610 231 24 0 2 232 58 288 1.24e-155 433
MsG0780039572.01.T01 MTR_7g074730 77.974 227 48 1 8 232 102 328 6.29e-136 385
MsG0780039572.01.T01 MTR_7g074820 75.877 228 52 2 8 232 102 329 3.83e-128 365
MsG0780039572.01.T01 MTR_7g074850 74.561 228 55 1 8 232 102 329 5.37e-128 365
MsG0780039572.01.T01 MTR_7g074710 68.000 225 72 0 8 232 102 326 2.83e-114 330
MsG0780039572.01.T01 MTR_4g023730 46.256 227 120 2 8 232 103 329 7.04e-63 199
MsG0780039572.01.T01 MTR_1g022440 44.444 225 121 3 10 232 103 325 2.32e-62 198
MsG0780039572.01.T01 MTR_1g022445 44.934 227 121 3 8 232 101 325 4.06e-60 192
MsG0780039572.01.T01 MTR_4g092080 38.627 233 131 3 8 230 106 336 2.35e-51 170
MsG0780039572.01.T01 MTR_4g081440 36.364 242 139 4 3 232 116 354 6.61e-50 167
MsG0780039572.01.T01 MTR_4g081440 36.364 242 138 5 3 232 112 349 1.45e-48 163
MsG0780039572.01.T01 MTR_5g056600 33.333 225 141 5 11 232 101 319 8.17e-39 137
MsG0780039572.01.T01 MTR_2g101330 36.842 228 130 8 10 232 101 319 9.91e-38 134
MsG0780039572.01.T01 MTR_3g031650 34.052 232 131 7 10 232 101 319 5.43e-31 116
MsG0780039572.01.T01 MTR_5g072620 34.310 239 135 7 3 232 94 319 7.58e-31 116
MsG0780039572.01.T01 MTR_3g005210 32.609 230 136 7 11 232 102 320 1.05e-30 115
MsG0780039572.01.T01 MTR_8g062110 33.929 224 134 7 11 229 19 233 1.18e-30 113
MsG0780039572.01.T01 MTR_2g013230 32.932 249 142 8 4 232 7 250 2.37e-30 113
MsG0780039572.01.T01 MTR_8g062110 33.778 225 135 7 10 229 104 319 9.21e-30 113
MsG0780039572.01.T01 MTR_8g062110 33.482 224 135 6 11 229 114 328 1.31e-29 113
MsG0780039572.01.T01 MTR_2g013230 32.932 249 142 8 4 232 97 340 2.24e-29 112
MsG0780039572.01.T01 MTR_8g062110 34.659 176 107 3 10 182 18 188 1.04e-27 104
MsG0780039572.01.T01 MTR_3g005170 30.435 230 141 7 10 231 74 292 8.11e-27 105
MsG0780039572.01.T01 MTR_8g062110 34.659 176 107 3 10 182 104 274 8.90e-27 104
MsG0780039572.01.T01 MTR_8g062110 34.659 176 107 3 10 182 104 274 9.27e-27 104
MsG0780039572.01.T01 MTR_4g009040 31.111 225 144 5 10 232 108 323 9.46e-27 105
MsG0780039572.01.T01 MTR_4g009040 31.111 225 144 5 10 232 112 327 9.76e-27 105
MsG0780039572.01.T01 MTR_3g005170 30.435 230 141 7 10 231 105 323 1.97e-26 104
MsG0780039572.01.T01 MTR_4g077130 32.018 228 138 8 11 231 105 322 1.56e-25 102
MsG0780039572.01.T01 MTR_8g062440 31.579 228 142 7 10 232 103 321 1.68e-25 102
MsG0780039572.01.T01 MTR_8g062440 31.579 228 142 7 10 232 104 322 1.79e-25 102
MsG0780039572.01.T01 MTR_2g013250 29.921 254 150 7 1 230 88 337 6.42e-25 100
MsG0780039572.01.T01 MTR_2g028620 31.276 243 138 8 3 232 100 326 8.13e-25 100
MsG0780039572.01.T01 MTR_3g005170 32.768 177 112 4 10 183 105 277 9.58e-25 99.4
MsG0780039572.01.T01 MTR_2g104960 33.040 227 141 8 10 232 110 329 1.62e-24 99.8
MsG0780039572.01.T01 MTR_3g031650 35.028 177 105 4 10 182 101 271 2.62e-24 97.8
MsG0780039572.01.T01 MTR_7g117190 30.952 252 144 8 4 232 96 340 1.57e-23 97.1
MsG0780039572.01.T01 MTR_7g117190 31.915 235 130 8 21 232 3 230 1.92e-23 94.7
MsG0780039572.01.T01 MTR_4g006940 30.804 224 148 4 11 232 108 326 2.33e-23 96.7
MsG0780039572.01.T01 MTR_8g062440 33.523 176 109 4 10 182 103 273 1.11e-22 94.4
MsG0780039572.01.T01 MTR_2g028620 34.574 188 112 5 3 185 100 281 2.03e-22 93.6
MsG0780039572.01.T01 MTR_4g009690 32.620 187 117 4 46 232 145 322 9.56e-21 89.4
MsG0780039572.01.T01 MTR_2g013260 30.894 246 147 7 4 230 26 267 1.36e-20 88.2
MsG0780039572.01.T01 MTR_4g009690 31.551 187 120 3 46 232 145 323 1.77e-20 88.6
MsG0780039572.01.T01 MTR_2g013260 31.102 254 147 8 1 230 89 338 1.92e-20 88.6
MsG0780039572.01.T01 MTR_4g006940 31.250 176 115 3 11 184 108 279 1.36e-18 82.8
MsG0780039572.01.T01 MTR_4g006940 31.250 176 115 3 11 184 108 279 1.36e-18 82.8
MsG0780039572.01.T01 MTR_4g077100 30.682 176 115 5 11 183 107 278 2.48e-18 82.0
MsG0780039572.01.T01 MTR_4g077100 31.250 176 114 6 11 183 107 278 3.22e-18 82.4
MsG0780039572.01.T01 MTR_7g117190 31.188 202 112 7 4 182 96 293 6.20e-18 81.3
MsG0780039572.01.T01 MTR_6g406250 30.667 150 91 4 17 159 157 300 9.43e-18 81.3
MsG0780039572.01.T01 MTR_2g013260 30.583 206 118 7 1 182 89 293 2.09e-13 68.6
MsG0780039572.01.T01 MTR_6g406250 32.323 99 59 2 17 110 157 252 3.14e-13 67.4
MsG0780039572.01.T01 MTR_0685s0030 30.702 114 78 1 70 183 3 115 1.01e-12 63.2
MsG0780039572.01.T01 MTR_0685s0030 30.702 114 78 1 70 183 3 115 2.23e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039572.01.T01 AT2G45400 44.304 237 125 4 2 232 72 307 1.54e-65 205
MsG0780039572.01.T01 AT2G45400 44.304 237 125 4 2 232 129 364 5.29e-65 206
MsG0780039572.01.T01 AT5G42800 43.723 231 118 3 8 232 101 325 9.54e-60 193
MsG0780039572.01.T01 AT2G45400 45.026 191 99 3 2 186 129 319 1.89e-52 172
MsG0780039572.01.T01 AT4G35420 37.885 227 129 7 10 232 103 321 1.26e-45 155
MsG0780039572.01.T01 AT1G61720 37.603 242 135 6 3 232 100 337 5.63e-44 151
MsG0780039572.01.T01 AT1G25460 33.193 238 148 6 1 232 87 319 1.71e-36 130
MsG0780039572.01.T01 AT2G02400 35.498 231 127 7 11 232 101 318 1.33e-35 128
MsG0780039572.01.T01 AT4G27250 31.140 228 140 5 10 223 113 337 2.93e-35 128
MsG0780039572.01.T01 AT1G68540 32.743 226 143 5 11 232 101 321 3.71e-35 127
MsG0780039572.01.T01 AT1G68540 32.877 219 138 5 18 232 1 214 2.26e-34 122
MsG0780039572.01.T01 AT4G27250 31.507 219 139 5 11 223 114 327 3.84e-34 125
MsG0780039572.01.T01 AT5G58490 35.169 236 137 8 3 232 98 323 2.37e-32 120
MsG0780039572.01.T01 AT1G09480 33.778 225 140 6 11 232 104 322 1.00e-31 118
MsG0780039572.01.T01 AT1G09480 33.778 225 140 6 11 232 151 369 2.68e-31 118
MsG0780039572.01.T01 AT1G09510 35.111 225 131 7 11 229 104 319 6.33e-30 114
MsG0780039572.01.T01 AT1G09490 32.444 225 143 6 11 232 73 291 1.58e-29 112
MsG0780039572.01.T01 AT1G09490 32.444 225 143 6 11 232 104 322 3.61e-29 112
MsG0780039572.01.T01 AT2G45400 44.444 126 65 2 2 122 129 254 6.74e-29 109
MsG0780039572.01.T01 AT1G51410 30.837 227 146 7 10 232 104 323 2.78e-27 107
MsG0780039572.01.T01 AT5G19440 32.174 230 137 7 10 231 105 323 2.11e-26 104
MsG0780039572.01.T01 AT1G15950 33.043 230 137 6 10 232 5 224 2.03e-25 100
MsG0780039572.01.T01 AT2G33590 31.250 224 130 9 10 224 104 312 6.78e-25 100
MsG0780039572.01.T01 AT1G09500 30.493 223 146 6 11 229 71 288 8.52e-25 99.8
MsG0780039572.01.T01 AT1G09500 30.493 223 146 6 11 229 105 322 1.33e-24 99.8
MsG0780039572.01.T01 AT1G15950 33.043 230 137 6 10 232 103 322 1.67e-24 99.8
MsG0780039572.01.T01 AT1G15950 33.043 230 137 6 10 232 103 322 1.67e-24 99.8
MsG0780039572.01.T01 AT1G66800 31.081 222 146 6 11 232 104 318 3.74e-24 98.6
MsG0780039572.01.T01 AT1G09500 32.000 175 112 4 11 182 105 275 3.81e-24 97.4
MsG0780039572.01.T01 AT2G33600 33.636 220 130 8 10 224 104 312 1.01e-21 91.7
MsG0780039572.01.T01 AT1G15950 31.455 213 140 3 10 220 103 311 1.83e-21 91.3
MsG0780039572.01.T01 AT1G76470 29.204 226 156 3 8 232 100 322 1.76e-20 88.6
MsG0780039572.01.T01 AT2G23910 29.327 208 130 8 11 209 69 268 5.72e-20 86.3
MsG0780039572.01.T01 AT1G80820 29.464 224 151 4 11 232 99 317 7.36e-20 87.0
MsG0780039572.01.T01 AT2G23910 29.327 208 130 8 11 209 103 302 1.11e-19 85.9
MsG0780039572.01.T01 AT1G15950 33.333 177 112 3 10 184 103 275 9.65e-19 83.2
MsG0780039572.01.T01 AT1G15950 33.333 177 112 3 10 184 103 275 1.40e-18 82.8
MsG0780039572.01.T01 AT1G15950 33.333 177 112 3 10 184 103 275 1.56e-18 82.8
MsG0780039572.01.T01 AT4G30470 28.846 208 129 7 11 208 102 300 1.18e-17 80.5
MsG0780039572.01.T01 AT2G23910 28.814 177 115 6 11 183 103 272 7.76e-17 77.8
MsG0780039572.01.T01 AT2G23910 28.814 177 115 6 11 183 103 272 8.48e-17 77.8
MsG0780039572.01.T01 AT5G14700 28.358 201 127 5 17 206 161 355 1.58e-16 77.8
MsG0780039572.01.T01 AT1G80820 30.899 178 113 5 11 184 99 270 5.37e-15 72.8

Find 38 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCTCTGTCTTTGTTATT+TGG 0.159764 7:-66038035 MsG0780039572.01.T01:intron
TATATGAAATTCAGGGTATT+TGG 0.255630 7:+66036145 None:intergenic
GAGAAGGCTGTTCTAGAGTT+TGG 0.265629 7:-66038146 MsG0780039572.01.T01:CDS
GAGCAATTATATCTCCCTTT+TGG 0.266827 7:+66036212 None:intergenic
AGTGTTGGTATATGAAATTC+AGG 0.275539 7:+66036137 None:intergenic
AGAACAATTGATGGAGCATT+AGG 0.319772 7:-66038353 MsG0780039572.01.T01:CDS
AGGGAGCTGAAGGAAATTAA+AGG 0.350467 7:-66036016 MsG0780039572.01.T01:CDS
ATGTAATGGATGAGAGTTAT+TGG 0.372531 7:-66038238 MsG0780039572.01.T01:CDS
GATTCAGTGTTGCAAGGAAA+AGG 0.394699 7:-66035906 MsG0780039572.01.T01:CDS
AGTGACAAAAGAACAATTGA+TGG 0.397128 7:-66038362 MsG0780039572.01.T01:CDS
TCTTCAATATTTGCAATGAA+TGG 0.401037 7:+66036188 None:intergenic
TGGCTTCTCTCGTCTCCATA+TGG 0.409487 7:-66036288 MsG0780039572.01.T01:CDS
GTAGGGGACAAGAATCCATT+TGG 0.425747 7:-66036308 MsG0780039572.01.T01:intron
CAAGTGCTTCAGCAGTGTAT+TGG 0.428274 7:-66038277 MsG0780039572.01.T01:CDS
ATTGAATATGACCAAGCAAA+TGG 0.453024 7:+66038185 None:intergenic
TATCATCAACATGCACCATA+TGG 0.453095 7:+66036273 None:intergenic
CTTGAGCATCCTAATCCAAA+AGG 0.458566 7:-66036227 MsG0780039572.01.T01:CDS
GAGATTTGAAACCATTTGCT+TGG 0.459886 7:-66038196 MsG0780039572.01.T01:CDS
TTCTAAAACAATGGCAGAGA+AGG 0.467203 7:-66038162 MsG0780039572.01.T01:CDS
TTCATTTGTCCTAAGCGTCC+TGG 0.480298 7:-66038068 MsG0780039572.01.T01:CDS
TAAATTGAGCCAGGACGCTT+AGG 0.480492 7:+66038059 None:intergenic
ATTCAGTGTTGCAAGGAAAA+GGG 0.509113 7:-66035905 MsG0780039572.01.T01:CDS
TGGAGACGAGAGAAGCCAAA+TGG 0.520904 7:+66036293 None:intergenic
AGGACGCTTAGGACAAATGA+AGG 0.523329 7:+66038070 None:intergenic
ATATTCAATTTCTAAAACAA+TGG 0.525829 7:-66038171 MsG0780039572.01.T01:CDS
AGATAAAGATGATGATGTAA+TGG 0.533409 7:-66038252 MsG0780039572.01.T01:CDS
ACACAGCCTTGAGGAAATGT+TGG 0.550570 7:-66035936 MsG0780039572.01.T01:CDS
AAGGGTCCAAGAACAAAAGT+TGG 0.557011 7:+66038089 None:intergenic
TTGAGCATCCTAATCCAAAA+GGG 0.569459 7:-66036226 MsG0780039572.01.T01:CDS
CTAGAGTTTGGAGAACAACA+TGG 0.574839 7:-66038134 MsG0780039572.01.T01:CDS
AGAGAGGTATAAATTGAGCC+AGG 0.580168 7:+66038050 None:intergenic
ATTAGAACTCACTCTAGTGT+TGG 0.602184 7:+66036122 None:intergenic
GTGTTGGTATATGAAATTCA+GGG 0.639563 7:+66036138 None:intergenic
CGATACGATTCAGTGTTGCA+AGG 0.647384 7:-66035912 MsG0780039572.01.T01:CDS
TATCGTCCAACATTTCCTCA+AGG 0.653115 7:+66035930 None:intergenic
ACCAAATAACAAAGACAGAG+AGG 0.665667 7:+66038034 None:intergenic
GGACGCTTAGGACAAATGAA+GGG 0.710235 7:+66038071 None:intergenic
CGAGTTCAAACACAGCCTTG+AGG 0.724622 7:-66035945 MsG0780039572.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATATAAATTGATATATT+TGG - Chr7:66036500-66036519 MsG0780039572.01.T01:intron 10.0%
!!! GTTTTTTTTAATAAAAATTG+GGG - Chr7:66036317-66036336 MsG0780039572.01.T01:intron 10.0%
!! CATATAAAAAAAACATTTGT+TGG - Chr7:66037690-66037709 MsG0780039572.01.T01:intron 15.0%
!! TATTATTAGTGAAAAGATTA+TGG + Chr7:66036919-66036938 None:intergenic 15.0%
!!! ATACCAATAAATTCATTTTT+AGG - Chr7:66037888-66037907 MsG0780039572.01.T01:intron 15.0%
!!! ATATTCAATTTCTAAAACAA+TGG - Chr7:66036101-66036120 MsG0780039572.01.T01:intron 15.0%
!!! ATGACAATACTTTTTTTTAA+CGG + Chr7:66037827-66037846 None:intergenic 15.0%
!!! ATGTGTTTTTAAATTATAAG+TGG + Chr7:66037413-66037432 None:intergenic 15.0%
!!! ATTTATTCATTTTGTTCATA+GGG - Chr7:66038237-66038256 MsG0780039572.01.T01:CDS 15.0%
!!! TATTTATTCATTTTGTTCAT+AGG - Chr7:66038236-66038255 MsG0780039572.01.T01:CDS 15.0%
!!! TCTAAAATATAAGCAAAAAT+TGG + Chr7:66036562-66036581 None:intergenic 15.0%
!!! TGTGTTTTTAAATTATAAGT+GGG + Chr7:66037412-66037431 None:intergenic 15.0%
!!! TTTTTGCTTATATTTTAGAA+CGG - Chr7:66036563-66036582 MsG0780039572.01.T01:intron 15.0%
!! AGATAAGTTGTAAAATATAG+TGG + Chr7:66037103-66037122 None:intergenic 20.0%
!! ATAATAGTCAGAAGAAAAAA+AGG + Chr7:66036286-66036305 None:intergenic 20.0%
!! ATGCCTAAAAATGAATTTAT+TGG + Chr7:66037894-66037913 None:intergenic 20.0%
!! TAAATACTAGTTACAGTAAA+AGG + Chr7:66038222-66038241 None:intergenic 20.0%
!! TGAATTCTACATATTAGATT+GGG + Chr7:66037862-66037881 None:intergenic 20.0%
!! TGCTAATTAAGAATAGAAAA+TGG - Chr7:66037936-66037955 MsG0780039572.01.T01:intron 20.0%
!!! AACTAATGATCATTTTTCAA+CGG - Chr7:66036368-66036387 MsG0780039572.01.T01:intron 20.0%
!!! TGATTTTATAACCTCAAAAA+CGG + Chr7:66036414-66036433 None:intergenic 20.0%
!!! TGCATAAACTAATTTTCTAA+AGG + Chr7:66037748-66037767 None:intergenic 20.0%
!!! TTTTGCTTATATTTTAGAAC+GGG - Chr7:66036564-66036583 MsG0780039572.01.T01:intron 20.0%
! AAAGAGAGAGAAGATAAAAT+TGG - Chr7:66036650-66036669 MsG0780039572.01.T01:intron 25.0%
! AACAATATGTTTATAAGTGG+AGG + Chr7:66037175-66037194 None:intergenic 25.0%
! AATAGAAAATGGATGTTGTA+GGG - Chr7:66037947-66037966 MsG0780039572.01.T01:intron 25.0%
! ACAACTTATCTTAGAAATCA+TGG - Chr7:66037112-66037131 MsG0780039572.01.T01:intron 25.0%
! AGGAAACTAAGATTCATTTA+AGG + Chr7:66037728-66037747 None:intergenic 25.0%
! CCACTTATAAACATATTGTT+AGG - Chr7:66037175-66037194 MsG0780039572.01.T01:intron 25.0%
! CCTAACAATATGTTTATAAG+TGG + Chr7:66037178-66037197 None:intergenic 25.0%
! CTAATATGTAGAATTCACAA+TGG - Chr7:66037865-66037884 MsG0780039572.01.T01:intron 25.0%
! GTGAATTCTACATATTAGAT+TGG + Chr7:66037863-66037882 None:intergenic 25.0%
! TCTTCAATATTTGCAATGAA+TGG + Chr7:66038087-66038106 None:intergenic 25.0%
! TGTATCTAATTATGTGGATT+AGG - Chr7:66036686-66036705 MsG0780039572.01.T01:intron 25.0%
! TGTTAGTGTATCTAATTATG+TGG - Chr7:66036680-66036699 MsG0780039572.01.T01:intron 25.0%
! TTATCTTAGAAATCATGGTT+AGG - Chr7:66037117-66037136 MsG0780039572.01.T01:intron 25.0%
! TTTAACCTCAAAAAAACTCA+TGG - Chr7:66038294-66038313 MsG0780039572.01.T01:CDS 25.0%
! TTTAATAAAAATTGGGGAAG+AGG - Chr7:66036323-66036342 MsG0780039572.01.T01:intron 25.0%
!! AAAAACCATCAAATTAGCTT+TGG - Chr7:66036622-66036641 MsG0780039572.01.T01:intron 25.0%
!! AGATAAAGATGATGATGTAA+TGG - Chr7:66036020-66036039 MsG0780039572.01.T01:intron 25.0%
!! ATAATACCAACTTTTGTTCT+TGG - Chr7:66036177-66036196 MsG0780039572.01.T01:CDS 25.0%
!! ATATTTTGTCAGTATTGTGT+TGG + Chr7:66037660-66037679 None:intergenic 25.0%
!! CATTTTTACAATGAAACTAG+AGG + Chr7:66037918-66037937 None:intergenic 25.0%
!! GTATCTAATTATGTGGATTA+GGG - Chr7:66036687-66036706 MsG0780039572.01.T01:intron 25.0%
!! TATATGAAATTCAGGGTATT+TGG + Chr7:66038130-66038149 None:intergenic 25.0%
!! TATTTTGTCAGTATTGTGTT+GGG + Chr7:66037659-66037678 None:intergenic 25.0%
!!! AATTTAGCTTGTTGTTCTAA+CGG - Chr7:66037035-66037054 MsG0780039572.01.T01:intron 25.0%
!!! ACTCATTTTTGCTTACATTT+TGG + Chr7:66036481-66036500 None:intergenic 25.0%
!!! AGTTGTTAAAAGTGTCAAAT+CGG + Chr7:66037454-66037473 None:intergenic 25.0%
!!! AGTTTTTTTGAGGTTAAATG+TGG + Chr7:66038292-66038311 None:intergenic 25.0%
!!! CTCATTTTTGCTTACATTTT+GGG + Chr7:66036480-66036499 None:intergenic 25.0%
!!! TTTGCTTATATTTTAGAACG+GGG - Chr7:66036565-66036584 MsG0780039572.01.T01:intron 25.0%
AACCTCATCAAAATTGTAAC+TGG - Chr7:66037478-66037497 MsG0780039572.01.T01:intron 30.0%
AGTGACAAAAGAACAATTGA+TGG - Chr7:66035910-66035929 MsG0780039572.01.T01:CDS 30.0%
ATAGAAAATGGATGTTGTAG+GGG - Chr7:66037948-66037967 MsG0780039572.01.T01:intron 30.0%
ATTGAATATGACCAAGCAAA+TGG + Chr7:66036090-66036109 None:intergenic 30.0%
CAAAATTGTAACTGGTTGAT+TGG - Chr7:66037486-66037505 MsG0780039572.01.T01:intron 30.0%
CAACTTGTATTTAAATACCG+CGG + Chr7:66036885-66036904 None:intergenic 30.0%
CATTTCCAAAGCTAATTTGA+TGG + Chr7:66036630-66036649 None:intergenic 30.0%
GAATAGAAAATGGATGTTGT+AGG - Chr7:66037946-66037965 MsG0780039572.01.T01:intron 30.0%
GATTGTTGAGTATGACTTAT+AGG - Chr7:66036737-66036756 MsG0780039572.01.T01:intron 30.0%
! AAATGTTTGGCTCAAGTTTA+AGG + Chr7:66036810-66036829 None:intergenic 30.0%
! AATGTTTGGCTCAAGTTTAA+GGG + Chr7:66036809-66036828 None:intergenic 30.0%
! AATTAGTTTATGCAGTTGTG+TGG - Chr7:66037754-66037773 MsG0780039572.01.T01:intron 30.0%
! GAAGCTGTTGTTTATAAATG+TGG - Chr7:66036345-66036364 MsG0780039572.01.T01:intron 30.0%
! GTGTTGGTATATGAAATTCA+GGG + Chr7:66038137-66038156 None:intergenic 30.0%
! TTATATCTCCCTTTTGGATT+AGG + Chr7:66038057-66038076 None:intergenic 30.0%
!! AACCAGTTACAATTTTGATG+AGG + Chr7:66037483-66037502 None:intergenic 30.0%
!! AAGATGTTTGATAGTGAAGT+TGG + Chr7:66036977-66036996 None:intergenic 30.0%
!! AGATGTTTGATAGTGAAGTT+GGG + Chr7:66036976-66036995 None:intergenic 30.0%
!! AGTGTTGGTATATGAAATTC+AGG + Chr7:66038138-66038157 None:intergenic 30.0%
!! ATCACATAAGTCAGTTTTGT+GGG + Chr7:66037379-66037398 None:intergenic 30.0%
!! ATGTAATGGATGAGAGTTAT+TGG - Chr7:66036034-66036053 MsG0780039572.01.T01:intron 30.0%
!! ATTTTTCAACGGACTCAAAT+GGG - Chr7:66036379-66036398 MsG0780039572.01.T01:intron 30.0%
!! TTAAAAGTGTCAAATCGGAT+AGG + Chr7:66037449-66037468 None:intergenic 30.0%
!! TTTTTGCTTACATTTTGGGA+CGG + Chr7:66036476-66036495 None:intergenic 30.0%
!!! TTGCTTATATTTTAGAACGG+GGG - Chr7:66036566-66036585 MsG0780039572.01.T01:intron 30.0%
ACCAAATAACAAAGACAGAG+AGG + Chr7:66036241-66036260 None:intergenic 35.0%
AGAGTCTCTTCAAAATCCAT+CGG + Chr7:66037318-66037337 None:intergenic 35.0%
ATTAGAACTCACTCTAGTGT+TGG + Chr7:66038153-66038172 None:intergenic 35.0%
ATTCAGTGTTGCAAGGAAAA+GGG - Chr7:66038367-66038386 MsG0780039572.01.T01:CDS 35.0%
CAGTTCAATGATGCTATAGT+TGG - Chr7:66036858-66036877 MsG0780039572.01.T01:intron 35.0%
CGCGGTATTTAAATACAAGT+TGG - Chr7:66036883-66036902 MsG0780039572.01.T01:intron 35.0%
GTTGTGTGGAAGAATACATT+TGG - Chr7:66037768-66037787 MsG0780039572.01.T01:intron 35.0%
TATCATCAACATGCACCATA+TGG + Chr7:66038002-66038021 None:intergenic 35.0%
TCAAAAAAACTCATGGATGC+TGG - Chr7:66038301-66038320 MsG0780039572.01.T01:CDS 35.0%
TGATACCATCTTAGAAATCG+TGG - Chr7:66037337-66037356 MsG0780039572.01.T01:intron 35.0%
TGCATTAGATGTCAACTTAG+TGG + Chr7:66036445-66036464 None:intergenic 35.0%
TGTTCGTGAACATAAATGGT+GGG - Chr7:66037259-66037278 MsG0780039572.01.T01:intron 35.0%
TTGAGCATCCTAATCCAAAA+GGG - Chr7:66038046-66038065 MsG0780039572.01.T01:CDS 35.0%
TTGTTCGTGAACATAAATGG+TGG - Chr7:66037258-66037277 MsG0780039572.01.T01:intron 35.0%
! AACATTTGTTGGAAGATGTC+AGG - Chr7:66037701-66037720 MsG0780039572.01.T01:intron 35.0%
! AGAACAATTGATGGAGCATT+AGG - Chr7:66035919-66035938 MsG0780039572.01.T01:CDS 35.0%
! CATCACATAAGTCAGTTTTG+TGG + Chr7:66037380-66037399 None:intergenic 35.0%
! GAGATTTGAAACCATTTGCT+TGG - Chr7:66036076-66036095 MsG0780039572.01.T01:intron 35.0%
! GAGCAATTATATCTCCCTTT+TGG + Chr7:66038063-66038082 None:intergenic 35.0%
! GCTTTGTTCGTGAACATAAA+TGG - Chr7:66037255-66037274 MsG0780039572.01.T01:intron 35.0%
! TCTAACCACGATTTCTAAGA+TGG + Chr7:66037345-66037364 None:intergenic 35.0%
!! ACCTCTCTGTCTTTGTTATT+TGG - Chr7:66036237-66036256 MsG0780039572.01.T01:CDS 35.0%
!! CATTTTTCAACGGACTCAAA+TGG - Chr7:66036378-66036397 MsG0780039572.01.T01:intron 35.0%
!! TTCTAAAACAATGGCAGAGA+AGG - Chr7:66036110-66036129 MsG0780039572.01.T01:intron 35.0%
!! TTGAGCCAAACATTTGTTGT+GGG - Chr7:66036815-66036834 MsG0780039572.01.T01:intron 35.0%
!!! AGCATCCATGAGTTTTTTTG+AGG + Chr7:66038302-66038321 None:intergenic 35.0%
AAGGGTCCAAGAACAAAAGT+TGG + Chr7:66036186-66036205 None:intergenic 40.0%
AGGGAGCTGAAGGAAATTAA+AGG - Chr7:66038256-66038275 MsG0780039572.01.T01:CDS 40.0%
CTAGAGTTTGGAGAACAACA+TGG - Chr7:66036138-66036157 MsG0780039572.01.T01:CDS 40.0%
CTTGAGCATCCTAATCCAAA+AGG - Chr7:66038045-66038064 MsG0780039572.01.T01:CDS 40.0%
GAGTCTCTTCAAAATCCATC+GGG + Chr7:66037317-66037336 None:intergenic 40.0%
GATTCAGTGTTGCAAGGAAA+AGG - Chr7:66038366-66038385 MsG0780039572.01.T01:CDS 40.0%
GTGTGTTAAGAGTCTCACAT+CGG + Chr7:66037212-66037231 None:intergenic 40.0%
TATCGTCCAACATTTCCTCA+AGG + Chr7:66038345-66038364 None:intergenic 40.0%
TTAAGAGTCTCACATCGGAT+AGG + Chr7:66037207-66037226 None:intergenic 40.0%
TTGTCCCCACAACAAATGTT+TGG + Chr7:66036823-66036842 None:intergenic 40.0%
! AGAGAGGTATAAATTGAGCC+AGG + Chr7:66036225-66036244 None:intergenic 40.0%
! CTTGAGCCAAACATTTGTTG+TGG - Chr7:66036814-66036833 MsG0780039572.01.T01:intron 40.0%
! TTTTGTTCATAGGGAGCTGA+AGG - Chr7:66038246-66038265 MsG0780039572.01.T01:CDS 40.0%
!! ATAGGTACTGAGAAGTGAGA+AGG - Chr7:66036755-66036774 MsG0780039572.01.T01:intron 40.0%
!! GGATTTGAACTCCGTTTTTG+AGG - Chr7:66036400-66036419 MsG0780039572.01.T01:intron 40.0%
!! TAGGTACTGAGAAGTGAGAA+GGG - Chr7:66036756-66036775 MsG0780039572.01.T01:intron 40.0%
!! TATGTGGATTAGGGATCAGT+TGG - Chr7:66036696-66036715 MsG0780039572.01.T01:intron 40.0%
!! TGAGCCAAACATTTGTTGTG+GGG - Chr7:66036816-66036835 MsG0780039572.01.T01:intron 40.0%
ACACAGCCTTGAGGAAATGT+TGG - Chr7:66038336-66038355 MsG0780039572.01.T01:CDS 45.0%
AGCATCATTGAACTGTACCG+CGG + Chr7:66036853-66036872 None:intergenic 45.0%
AGGACGCTTAGGACAAATGA+AGG + Chr7:66036205-66036224 None:intergenic 45.0%
ATGATGCTATAGTTGGACCG+CGG - Chr7:66036865-66036884 MsG0780039572.01.T01:intron 45.0%
ATGGTTAGGCCTAACACAAC+CGG - Chr7:66037131-66037150 MsG0780039572.01.T01:intron 45.0%
CAAGTGCTTCAGCAGTGTAT+TGG - Chr7:66035995-66036014 MsG0780039572.01.T01:CDS 45.0%
CGAACAAAGCCCAATAGTGT+TGG + Chr7:66037245-66037264 None:intergenic 45.0%
CGATACGATTCAGTGTTGCA+AGG - Chr7:66038360-66038379 MsG0780039572.01.T01:CDS 45.0%
GGACGCTTAGGACAAATGAA+GGG + Chr7:66036204-66036223 None:intergenic 45.0%
GTAGGGGACAAGAATCCATT+TGG - Chr7:66037964-66037983 MsG0780039572.01.T01:intron 45.0%
GTGTCAAATCGGATAGGAGA+TGG + Chr7:66037443-66037462 None:intergenic 45.0%
TAAATTGAGCCAGGACGCTT+AGG + Chr7:66036216-66036235 None:intergenic 45.0%
TAGGGATCAGTTGGTCAGTT+AGG - Chr7:66036705-66036724 MsG0780039572.01.T01:intron 45.0%
TTCATTTGTCCTAAGCGTCC+TGG - Chr7:66036204-66036223 MsG0780039572.01.T01:CDS 45.0%
! TCGTGAACATAAATGGTGGG+TGG - Chr7:66037262-66037281 MsG0780039572.01.T01:intron 45.0%
! TTTGGCTCAAGTTTAAGGGC+AGG + Chr7:66036805-66036824 None:intergenic 45.0%
!! GAGAAGGCTGTTCTAGAGTT+TGG - Chr7:66036126-66036145 MsG0780039572.01.T01:intron 45.0%
!!! TGCTTACATTTTGGGACGGA+GGG + Chr7:66036472-66036491 None:intergenic 45.0%
!!! TTGCTTACATTTTGGGACGG+AGG + Chr7:66036473-66036492 None:intergenic 45.0%
!!! TGTTTTTTTTAATAAAAATT+GGG - Chr7:66036316-66036335 MsG0780039572.01.T01:intron 5.0%
!!! TTGTTTTTTTTAATAAAAAT+TGG - Chr7:66036315-66036334 MsG0780039572.01.T01:intron 5.0%
AGGGTTGAATGAGCGAAGAG+AGG - Chr7:66036775-66036794 MsG0780039572.01.T01:intron 50.0%
CATCATTGAACTGTACCGCG+GGG + Chr7:66036851-66036870 None:intergenic 50.0%
CCCTCACGACCAACACTATT+GGG - Chr7:66037233-66037252 MsG0780039572.01.T01:intron 50.0%
CGAGTTCAAACACAGCCTTG+AGG - Chr7:66038327-66038346 MsG0780039572.01.T01:CDS 50.0%
GCATCATTGAACTGTACCGC+GGG + Chr7:66036852-66036871 None:intergenic 50.0%
GGGTTGAATGAGCGAAGAGA+GGG - Chr7:66036776-66036795 MsG0780039572.01.T01:intron 50.0%
GTCTCACATCGGATAGGAGA+TGG + Chr7:66037201-66037220 None:intergenic 50.0%
TGGAGACGAGAGAAGCCAAA+TGG + Chr7:66037982-66038001 None:intergenic 50.0%
TGGCTTCTCTCGTCTCCATA+TGG - Chr7:66037984-66038003 MsG0780039572.01.T01:intron 50.0%
!! AATGGTGGGTGGTCTGATAG+CGG - Chr7:66037273-66037292 MsG0780039572.01.T01:intron 50.0%
!! CAATAGTGTTGGTCGTGAGG+GGG + Chr7:66037234-66037253 None:intergenic 50.0%
!! CCAATAGTGTTGGTCGTGAG+GGG + Chr7:66037235-66037254 None:intergenic 50.0%
!! CCCAATAGTGTTGGTCGTGA+GGG + Chr7:66037236-66037255 None:intergenic 50.0%
!! GATAGCGGAAACCTGATGAC+AGG - Chr7:66037288-66037307 MsG0780039572.01.T01:intron 50.0%
AGCGGAAACCTGATGACAGG+TGG - Chr7:66037291-66037310 MsG0780039572.01.T01:intron 55.0%
CCCCTCACGACCAACACTAT+TGG - Chr7:66037232-66037251 MsG0780039572.01.T01:intron 55.0%
CCTCACAAGCCGGTTGTGTT+AGG + Chr7:66037143-66037162 None:intergenic 55.0%
GCAATCCTCACCTCACAAGC+CGG + Chr7:66037153-66037172 None:intergenic 55.0%
!! CCTAACACAACCGGCTTGTG+AGG - Chr7:66037140-66037159 MsG0780039572.01.T01:intron 55.0%
!! GCCCAATAGTGTTGGTCGTG+AGG + Chr7:66037237-66037256 None:intergenic 55.0%
!! CACAACCGGCTTGTGAGGTG+AGG - Chr7:66037145-66037164 MsG0780039572.01.T01:intron 60.0%
CCATCGGGCCACCTGTCATC+AGG + Chr7:66037302-66037321 None:intergenic 65.0%
CCTGATGACAGGTGGCCCGA+TGG - Chr7:66037299-66037318 MsG0780039572.01.T01:intron 65.0%
GTGGGGACAACGTGTCCCCG+CGG - Chr7:66036833-66036852 MsG0780039572.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 66035895 66038399 66035895 ID=MsG0780039572.01;Name=MsG0780039572.01
Chr7 mRNA 66035895 66038399 66035895 ID=MsG0780039572.01.T01;Parent=MsG0780039572.01;Name=MsG0780039572.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|0.5|0.66|3|0|232
Chr7 exon 66038036 66038399 66038036 ID=MsG0780039572.01.T01:exon:17957;Parent=MsG0780039572.01.T01
Chr7 exon 66036134 66036326 66036134 ID=MsG0780039572.01.T01:exon:17956;Parent=MsG0780039572.01.T01
Chr7 exon 66035895 66036036 66035895 ID=MsG0780039572.01.T01:exon:17955;Parent=MsG0780039572.01.T01
Chr7 CDS 66038036 66038399 66038036 ID=MsG0780039572.01.T01:cds;Parent=MsG0780039572.01.T01
Chr7 CDS 66036134 66036326 66036134 ID=MsG0780039572.01.T01:cds;Parent=MsG0780039572.01.T01
Chr7 CDS 66035895 66036036 66035895 ID=MsG0780039572.01.T01:cds;Parent=MsG0780039572.01.T01
Gene Sequence

>MsG0780039572.01.T01

ATGAATCAGAAGAACAGTGACAAAAGAACAATTGATGGAGCATTAGGAATTTTAAAAGCATGCAAAAATTCAAAGACAGTGAAGAGAGTTATTTACACTTCAAGTGCTTCAGCAGTGTATTGGCAAGATAAAGATGATGATGTAATGGATGAGAGTTATTGGAGTGATGAAAATCTTCTTAGAGATTTGAAACCATTTGCTTGGTCATATTCAATTTCTAAAACAATGGCAGAGAAGGCTGTTCTAGAGTTTGGAGAACAACATGGTTTAGATGTTGTGACTATAATACCAACTTTTGTTCTTGGACCCTTCATTTGTCCTAAGCGTCCTGGCTCAATTTATACCTCTCTGTCTTTGTTATTTGGGGACAAGAATCCATTTGGCTTCTCTCGTCTCCATATGGTGCATGTTGATGATATTGCAAGAGCACATATATTTCTGCTTGAGCATCCTAATCCAAAAGGGAGATATAATTGCTCACCATTCATTGCAAATATTGAAGAAATAGCTCAACTTATTTCTGCCAAATACCCTGAATTTCATATACCAACACTAGAGGAGCTGAAGGAAATTAAAGGTGACAAGCTTCCACATTTAACCTCAAAAAAACTCATGGATGCTGGATTCGAGTTCAAACACAGCCTTGAGGAAATGTTGGACGATACGATTCAGTGTTGCAAGGAAAAGGGTTATCTTTGA
Protein sequence

>MsG0780039572.01.T01

MNQKNSDKRTIDGALGILKACKNSKTVKRVIYTSSASAVYWQDKDDDVMDESYWSDENLLRDLKPFAWSYSISKTMAEKAVLEFGEQHGLDVVTIIPTFVLGPFICPKRPGSIYTSLSLLFGDKNPFGFSRLHMVHVDDIARAHIFLLEHPNPKGRYNCSPFIANIEEIAQLISAKYPEFHIPTLEELKEIKGDKLPHLTSKKLMDAGFEFKHSLEEMLDDTIQCCKEKGYL*