AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039723.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGGAAGAGGAGTGTTC+AGG 0.307247 7:+68120834 MsG0780039723.01.T01:CDS
AGGAAGATGAGGAGAGAGAA+AGG 0.344568 7:+68120966 MsG0780039723.01.T01:CDS
AGAGAAGAGATGGGAGGAGA+TGG 0.371834 7:+68120926 MsG0780039723.01.T01:CDS
GGAGTGGTGGGAGGTGGAAA+AGG 0.373850 7:+68120818 MsG0780039723.01.T01:CDS
GGAGATGGAGGTTGTGGTGA+AGG 0.387997 7:+68120941 MsG0780039723.01.T01:CDS
GTTGAAGGTGGTGGAGAGGG+TGG 0.412837 7:+68120872 MsG0780039723.01.T01:CDS
GGAGATGGAGTGGTGGGAGG+TGG 0.423532 7:+68120812 MsG0780039723.01.T01:CDS
AAAAGGAAGAGGAGTGTTCA+GGG 0.435233 7:+68120835 MsG0780039723.01.T01:CDS
TGTGATTGCGTTGTGGAAGA+TGG 0.449541 7:+68121013 MsG0780039723.01.T01:CDS
ATGGGAGGAGATGGAGGTTG+TGG 0.453574 7:+68120935 MsG0780039723.01.T01:CDS
GGTGGTGGAGAGGGTGGAGC+CGG 0.456049 7:+68120878 MsG0780039723.01.T01:CDS
TGTGATGGAGATGGAGTGGT+GGG 0.460188 7:+68120806 MsG0780039723.01.T01:CDS
GTGGGAGGTGGAAAAGGAAG+AGG 0.469870 7:+68120824 MsG0780039723.01.T01:CDS
CATCGATGGGAGAGAAGAGA+TGG 0.476441 7:+68120916 MsG0780039723.01.T01:CDS
GAAGAGATGGGAGGAGATGG+AGG 0.476784 7:+68120929 MsG0780039723.01.T01:CDS
GTTGTTGAAGGTGGTGGAGA+GGG 0.481458 7:+68120869 MsG0780039723.01.T01:CDS
TGGAGGTTGTGGTGAAGGAG+AGG 0.488529 7:+68120946 MsG0780039723.01.T01:CDS
TGAGAGGGAAGAAGATGAAG+AGG 0.490508 7:+68121039 MsG0780039723.01.T01:CDS
GGTGAAAAGACGGTTGTTGA+AGG 0.501824 7:+68120857 MsG0780039723.01.T01:CDS
AAGACGGTTGTTGAAGGTGG+TGG 0.503910 7:+68120863 MsG0780039723.01.T01:CDS
GGAAGATGAGGAGAGAGAAA+GGG 0.512203 7:+68120967 MsG0780039723.01.T01:CDS
TGGTGAAGGAGAGGAAGATG+AGG 0.513570 7:+68120955 MsG0780039723.01.T01:CDS
TTGTGATGGAGATGGAGTGG+TGG 0.529969 7:+68120805 MsG0780039723.01.T01:CDS
GATGGAGATGGAGTGGTGGG+AGG 0.533962 7:+68120809 MsG0780039723.01.T01:CDS
GGTTTGTGATGGAGATGGAG+TGG 0.568510 7:+68120802 MsG0780039723.01.T01:CDS
GTTGATGAGGTAGTCATCGA+TGG 0.568688 7:+68120902 MsG0780039723.01.T01:CDS
AGTGTTCAGGGGTGAAAAGA+CGG 0.589401 7:+68120847 MsG0780039723.01.T01:CDS
GAAAAGACGGTTGTTGAAGG+TGG 0.590110 7:+68120860 MsG0780039723.01.T01:CDS
AGATGAAGAGGAGAAGACTG+AGG 0.590127 7:+68121051 MsG0780039723.01.T01:CDS
GGGTGGAGCCGGTGTTGATG+AGG 0.590536 7:+68120889 MsG0780039723.01.T01:CDS
GGTTGTTGAAGGTGGTGGAG+AGG 0.590779 7:+68120868 MsG0780039723.01.T01:CDS
ATCGATGGGAGAGAAGAGAT+GGG 0.592932 7:+68120917 MsG0780039723.01.T01:CDS
AAAGGAAGAGGAGTGTTCAG+GGG 0.612905 7:+68120836 MsG0780039723.01.T01:CDS
TTGATGAGGTAGTCATCGAT+GGG 0.618603 7:+68120903 MsG0780039723.01.T01:CDS
TTGGGTTTGTGATTGCGTTG+TGG 0.620941 7:+68121006 MsG0780039723.01.T01:CDS
TGTGGAAGATGGTGATGAGA+GGG 0.632669 7:+68121024 MsG0780039723.01.T01:CDS
TTGTGGAAGATGGTGATGAG+AGG 0.654884 7:+68121023 MsG0780039723.01.T01:CDS
TGAAGAGGAGAAGACTGAGG+TGG 0.655498 7:+68121054 MsG0780039723.01.T01:CDS
GATGACTACCTCATCAACAC+CGG 0.677018 7:-68120897 None:intergenic
GATGGGAGAGAAGAGATGGG+AGG 0.718594 7:+68120920 MsG0780039723.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAAGGGTTGAAGATTTTCA+TGG + Chr7:68120983-68121002 MsG0780039723.01.T01:CDS 35.0%
! GGTTGAAGATTTTCATGGTT+GGG + Chr7:68120988-68121007 MsG0780039723.01.T01:CDS 35.0%
TTGATGAGGTAGTCATCGAT+GGG + Chr7:68120903-68120922 MsG0780039723.01.T01:CDS 40.0%
! GGGTTGAAGATTTTCATGGT+TGG + Chr7:68120987-68121006 MsG0780039723.01.T01:CDS 40.0%
!! AAAAGGAAGAGGAGTGTTCA+GGG + Chr7:68120835-68120854 MsG0780039723.01.T01:CDS 40.0%
AGATGAAGAGGAGAAGACTG+AGG + Chr7:68121051-68121070 MsG0780039723.01.T01:CDS 45.0%
AGGAAGATGAGGAGAGAGAA+AGG + Chr7:68120966-68120985 MsG0780039723.01.T01:CDS 45.0%
AGTGTTCAGGGGTGAAAAGA+CGG + Chr7:68120847-68120866 MsG0780039723.01.T01:CDS 45.0%
ATCGATGGGAGAGAAGAGAT+GGG + Chr7:68120917-68120936 MsG0780039723.01.T01:CDS 45.0%
GAAAAGACGGTTGTTGAAGG+TGG + Chr7:68120860-68120879 MsG0780039723.01.T01:CDS 45.0%
GATGACTACCTCATCAACAC+CGG - Chr7:68120900-68120919 None:intergenic 45.0%
GGAAGATGAGGAGAGAGAAA+GGG + Chr7:68120967-68120986 MsG0780039723.01.T01:CDS 45.0%
GGTGAAAAGACGGTTGTTGA+AGG + Chr7:68120857-68120876 MsG0780039723.01.T01:CDS 45.0%
TGAGAGGGAAGAAGATGAAG+AGG + Chr7:68121039-68121058 MsG0780039723.01.T01:CDS 45.0%
TGTGATTGCGTTGTGGAAGA+TGG + Chr7:68121013-68121032 MsG0780039723.01.T01:CDS 45.0%
TTGGGTTTGTGATTGCGTTG+TGG + Chr7:68121006-68121025 MsG0780039723.01.T01:CDS 45.0%
! GTTGATGAGGTAGTCATCGA+TGG + Chr7:68120902-68120921 MsG0780039723.01.T01:CDS 45.0%
! TGTGGAAGATGGTGATGAGA+GGG + Chr7:68121024-68121043 MsG0780039723.01.T01:CDS 45.0%
! TTGTGGAAGATGGTGATGAG+AGG + Chr7:68121023-68121042 MsG0780039723.01.T01:CDS 45.0%
!! AAAGGAAGAGGAGTGTTCAG+GGG + Chr7:68120836-68120855 MsG0780039723.01.T01:CDS 45.0%
!! GAAAAGGAAGAGGAGTGTTC+AGG + Chr7:68120834-68120853 MsG0780039723.01.T01:CDS 45.0%
AAGACGGTTGTTGAAGGTGG+TGG + Chr7:68120863-68120882 MsG0780039723.01.T01:CDS 50.0%
AGAGAAGAGATGGGAGGAGA+TGG + Chr7:68120926-68120945 MsG0780039723.01.T01:CDS 50.0%
CATCGATGGGAGAGAAGAGA+TGG + Chr7:68120916-68120935 MsG0780039723.01.T01:CDS 50.0%
TGAAGAGGAGAAGACTGAGG+TGG + Chr7:68121054-68121073 MsG0780039723.01.T01:CDS 50.0%
TGGTGAAGGAGAGGAAGATG+AGG + Chr7:68120955-68120974 MsG0780039723.01.T01:CDS 50.0%
! GGTTTGTGATGGAGATGGAG+TGG + Chr7:68120802-68120821 MsG0780039723.01.T01:CDS 50.0%
! TGTGATGGAGATGGAGTGGT+GGG + Chr7:68120806-68120825 MsG0780039723.01.T01:CDS 50.0%
! TTGTGATGGAGATGGAGTGG+TGG + Chr7:68120805-68120824 MsG0780039723.01.T01:CDS 50.0%
!! GTTGTTGAAGGTGGTGGAGA+GGG + Chr7:68120869-68120888 MsG0780039723.01.T01:CDS 50.0%
ATGGGAGGAGATGGAGGTTG+TGG + Chr7:68120935-68120954 MsG0780039723.01.T01:CDS 55.0%
GAAGAGATGGGAGGAGATGG+AGG + Chr7:68120929-68120948 MsG0780039723.01.T01:CDS 55.0%
GATGGGAGAGAAGAGATGGG+AGG + Chr7:68120920-68120939 MsG0780039723.01.T01:CDS 55.0%
GGAGATGGAGGTTGTGGTGA+AGG + Chr7:68120941-68120960 MsG0780039723.01.T01:CDS 55.0%
GTGGGAGGTGGAAAAGGAAG+AGG + Chr7:68120824-68120843 MsG0780039723.01.T01:CDS 55.0%
TGGAGGTTGTGGTGAAGGAG+AGG + Chr7:68120946-68120965 MsG0780039723.01.T01:CDS 55.0%
!! GGTTGTTGAAGGTGGTGGAG+AGG + Chr7:68120868-68120887 MsG0780039723.01.T01:CDS 55.0%
! GATGGAGATGGAGTGGTGGG+AGG + Chr7:68120809-68120828 MsG0780039723.01.T01:CDS 60.0%
! GGAGTGGTGGGAGGTGGAAA+AGG + Chr7:68120818-68120837 MsG0780039723.01.T01:CDS 60.0%
!! GTTGAAGGTGGTGGAGAGGG+TGG + Chr7:68120872-68120891 MsG0780039723.01.T01:CDS 60.0%
! GGAGATGGAGTGGTGGGAGG+TGG + Chr7:68120812-68120831 MsG0780039723.01.T01:CDS 65.0%
!! GGGTGGAGCCGGTGTTGATG+AGG + Chr7:68120889-68120908 MsG0780039723.01.T01:CDS 65.0%
!! GGTGGTGGAGAGGGTGGAGC+CGG + Chr7:68120878-68120897 MsG0780039723.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 68120800 68121081 68120800 ID=MsG0780039723.01;Name=MsG0780039723.01
Chr7 mRNA 68120800 68121081 68120800 ID=MsG0780039723.01.T01;Parent=MsG0780039723.01;Name=MsG0780039723.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|93
Chr7 exon 68120800 68121081 68120800 ID=MsG0780039723.01.T01:exon:30937;Parent=MsG0780039723.01.T01
Chr7 CDS 68120800 68121081 68120800 ID=MsG0780039723.01.T01:cds;Parent=MsG0780039723.01.T01
Gene Sequence

>MsG0780039723.01.T01

TTGGTTTGTGATGGAGATGGAGTGGTGGGAGGTGGAAAAGGAAGAGGAGTGTTCAGGGGTGAAAAGACGGTTGTTGAAGGTGGTGGAGAGGGTGGAGCCGGTGTTGATGAGGTAGTCATCGATGGGAGAGAAGAGATGGGAGGAGATGGAGGTTGTGGTGAAGGAGAGGAAGATGAGGAGAGAGAAAGGGTTGAAGATTTTCATGGTTGGGTTTGTGATTGCGTTGTGGAAGATGGTGATGAGAGGGAAGAAGATGAAGAGGAGAAGACTGAGGTGGCATGA
Protein sequence

>MsG0780039723.01.T01

LVCDGDGVVGGGKGRGVFRGEKTVVEGGGEGGAGVDEVVIDGREEMGGDGGCGEGEEDEERERVEDFHGWVCDCVVEDGDEREEDEEEKTEVA*