AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039959.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039959.01.T01 MTR_7g082300 87.778 90 11 0 1 90 33 122 3.43e-48 163
MsG0780039959.01.T01 MTR_7g082280 64.444 90 31 1 1 90 36 124 1.04e-32 119
MsG0780039959.01.T01 MTR_7g086800 56.044 91 38 2 1 91 31 119 1.53e-27 97.1
MsG0780039959.01.T01 MTR_7g082110 52.809 89 41 1 1 89 31 118 4.79e-24 94.7
MsG0780039959.01.T01 MTR_3g084510 52.809 89 40 2 1 89 36 122 4.43e-22 89.0
MsG0780039959.01.T01 MTR_8g083240 49.438 89 44 1 1 89 26 113 6.28e-22 88.6
MsG0780039959.01.T01 MTR_0394s0020 53.409 88 40 1 1 88 33 119 1.13e-17 76.3
MsG0780039959.01.T01 MTR_4g044393 54.651 86 38 1 1 86 33 117 2.36e-17 75.5
MsG0780039959.01.T01 MTR_5g009660 34.831 89 55 3 3 88 36 124 9.92e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039959.01.T01 AT2G01820 48.649 74 36 2 15 88 48 119 9.28e-15 68.2
MsG0780039959.01.T01 AT5G67280 44.118 68 37 1 22 88 64 131 9.60e-14 65.5
MsG0780039959.01.T01 AT3G23750 34.884 86 54 1 1 86 30 113 1.31e-12 62.0
MsG0780039959.01.T01 AT1G17240 40.964 83 46 2 11 90 71 153 6.46e-12 60.1
MsG0780039959.01.T01 AT1G17240 40.964 83 46 2 11 90 66 148 7.48e-12 60.1
MsG0780039959.01.T01 AT1G72300 39.759 83 47 2 11 90 68 150 4.28e-11 57.8

Find 29 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGACCAGCCTTTGGGAGTT+GGG 0.328264 7:+71107547 None:intergenic
CACGGTTTGGAGTGCGCTTA+GGG 0.396259 7:+71107338 None:intergenic
GGGTCAGTGCTTTCATAAGT+TGG 0.405492 7:+71107568 None:intergenic
TCACGGTTTGGAGTGCGCTT+AGG 0.415377 7:+71107337 None:intergenic
ACAACATTCACTACTGCAAA+TGG 0.419695 7:-71107524 MsG0780039959.01.T01:CDS
AGTAACCAAGGTTCACGGTT+TGG 0.431534 7:+71107325 None:intergenic
GGTCAGTGCTTTCATAAGTT+GGG 0.435450 7:+71107569 None:intergenic
AATGTTGTTAGACCAGCCTT+TGG 0.441289 7:+71107539 None:intergenic
TTAGACCAGCCTTTGGGAGT+TGG 0.444886 7:+71107546 None:intergenic
GCGCTTAGGGAAAACAAATC+AGG 0.454631 7:+71107351 None:intergenic
GTCCCTCTGAGTGAATTTGA+TGG 0.458291 7:+71107450 None:intergenic
CGGTGACAACTTGATTAGAT+TGG 0.471080 7:+71107484 None:intergenic
TGAATTTGATGGAAGCTTGA+TGG 0.473935 7:+71107461 None:intergenic
ATGTTGTTAGACCAGCCTTT+GGG 0.484264 7:+71107540 None:intergenic
AGACCAGCCTTTGGGAGTTG+GGG 0.499890 7:+71107548 None:intergenic
TTGAGAGAGTTGAAATTCTG+AGG 0.504341 7:+71107423 None:intergenic
CTTCATAACAACTTACTCAA+TGG 0.509574 7:-71107381 MsG0780039959.01.T01:CDS
CTAATAGTAACCAAGGTTCA+CGG 0.520511 7:+71107320 None:intergenic
TGACCCCAACTCCCAAAGGC+TGG 0.543489 7:-71107551 MsG0780039959.01.T01:CDS
TGAGAGAGTTGAAATTCTGA+GGG 0.545327 7:+71107424 None:intergenic
TTCCATCAAATTCACTCAGA+GGG 0.584484 7:-71107452 MsG0780039959.01.T01:CDS
GCACTCCAAACCGTGAACCT+TGG 0.597526 7:-71107330 MsG0780039959.01.T01:CDS
GCACTGACCCCAACTCCCAA+AGG 0.615425 7:-71107555 MsG0780039959.01.T01:CDS
AGGGAAAACAAATCAGGCAA+AGG 0.622947 7:+71107357 None:intergenic
ATTCACTACTGCAAATGGAA+CGG 0.635732 7:-71107519 MsG0780039959.01.T01:CDS
GTTGTTATGAAGATCGATGT+CGG 0.642002 7:+71107392 None:intergenic
CTATACTCTAATAGTAACCA+AGG 0.676588 7:+71107313 None:intergenic
CTTCCATCAAATTCACTCAG+AGG 0.687370 7:-71107453 MsG0780039959.01.T01:CDS
ATTTGATGGAAGCTTGATGG+CGG 0.712688 7:+71107464 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTTCATAACAACTTACTCAA+TGG - Chr7:71107512-71107531 MsG0780039959.01.T01:CDS 30.0%
ACAACATTCACTACTGCAAA+TGG - Chr7:71107369-71107388 MsG0780039959.01.T01:CDS 35.0%
ATTCACTACTGCAAATGGAA+CGG - Chr7:71107374-71107393 MsG0780039959.01.T01:CDS 35.0%
CTAATAGTAACCAAGGTTCA+CGG + Chr7:71107576-71107595 None:intergenic 35.0%
GTTGTTATGAAGATCGATGT+CGG + Chr7:71107504-71107523 None:intergenic 35.0%
TGAATTTGATGGAAGCTTGA+TGG + Chr7:71107435-71107454 None:intergenic 35.0%
TGAGAGAGTTGAAATTCTGA+GGG + Chr7:71107472-71107491 None:intergenic 35.0%
TTCCATCAAATTCACTCAGA+GGG - Chr7:71107441-71107460 MsG0780039959.01.T01:CDS 35.0%
TTGAGAGAGTTGAAATTCTG+AGG + Chr7:71107473-71107492 None:intergenic 35.0%
AATGTTGTTAGACCAGCCTT+TGG + Chr7:71107357-71107376 None:intergenic 40.0%
AGGGAAAACAAATCAGGCAA+AGG + Chr7:71107539-71107558 None:intergenic 40.0%
ATGTTGTTAGACCAGCCTTT+GGG + Chr7:71107356-71107375 None:intergenic 40.0%
ATTTGATGGAAGCTTGATGG+CGG + Chr7:71107432-71107451 None:intergenic 40.0%
CGGTGACAACTTGATTAGAT+TGG + Chr7:71107412-71107431 None:intergenic 40.0%
CTTCCATCAAATTCACTCAG+AGG - Chr7:71107440-71107459 MsG0780039959.01.T01:CDS 40.0%
GGTCAGTGCTTTCATAAGTT+GGG + Chr7:71107327-71107346 None:intergenic 40.0%
AGTAACCAAGGTTCACGGTT+TGG + Chr7:71107571-71107590 None:intergenic 45.0%
GCGCTTAGGGAAAACAAATC+AGG + Chr7:71107545-71107564 None:intergenic 45.0%
GGGTCAGTGCTTTCATAAGT+TGG + Chr7:71107328-71107347 None:intergenic 45.0%
GTCCCTCTGAGTGAATTTGA+TGG + Chr7:71107446-71107465 None:intergenic 45.0%
! TAGACCAGCCTTTGGGAGTT+GGG + Chr7:71107349-71107368 None:intergenic 50.0%
! TTAGACCAGCCTTTGGGAGT+TGG + Chr7:71107350-71107369 None:intergenic 50.0%
GCACTCCAAACCGTGAACCT+TGG - Chr7:71107563-71107582 MsG0780039959.01.T01:CDS 55.0%
! AGACCAGCCTTTGGGAGTTG+GGG + Chr7:71107348-71107367 None:intergenic 55.0%
! CACGGTTTGGAGTGCGCTTA+GGG + Chr7:71107558-71107577 None:intergenic 55.0%
! TCACGGTTTGGAGTGCGCTT+AGG + Chr7:71107559-71107578 None:intergenic 55.0%
TGACCCCAACTCCCAAAGGC+TGG - Chr7:71107342-71107361 MsG0780039959.01.T01:CDS 60.0%
! GCACTGACCCCAACTCCCAA+AGG - Chr7:71107338-71107357 MsG0780039959.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 71107320 71107595 71107320 ID=MsG0780039959.01;Name=MsG0780039959.01
Chr7 mRNA 71107320 71107595 71107320 ID=MsG0780039959.01.T01;Parent=MsG0780039959.01;Name=MsG0780039959.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|91
Chr7 exon 71107320 71107595 71107320 ID=MsG0780039959.01.T01:exon:13670;Parent=MsG0780039959.01.T01
Chr7 CDS 71107320 71107595 71107320 ID=MsG0780039959.01.T01:cds;Parent=MsG0780039959.01.T01
Gene Sequence

>MsG0780039959.01.T01

ATGTCCCAACTTATGAAAGCACTGACCCCAACTCCCAAAGGCTGGTCTAACAACATTCACTACTGCAAATGGAACGGCATCATGTGCGACCAATCTAATCAAGTTGTCACCGCCATCAAGCTTCCATCAAATTCACTCAGAGGGACAATCCCTCAGAATTTCAACTCTCTCAATAATCTCACCGACATCGATCTTCATAACAACTTACTCAATGGTCCTTTGCCTGATTTGTTTTCCCTAAGCGCACTCCAAACCGTGAACCTTGGTTACTATTAG
Protein sequence

>MsG0780039959.01.T01

MSQLMKALTPTPKGWSNNIHYCKWNGIMCDQSNQVVTAIKLPSNSLRGTIPQNFNSLNNLTDIDLHNNLLNGPLPDLFSLSALQTVNLGYY*