Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040487.01.T01 | XP_003625144.1 | 95.175 | 228 | 6 | 2 | 8 | 234 | 214 | 437 | 4.29E-152 | 440 |
| MsG0780040487.01.T02 | XP_003625144.1 | 95.946 | 222 | 5 | 1 | 1 | 222 | 220 | 437 | 2.50E-149 | 432 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040487.01.T01 | G7KTI4 | 95.175 | 228 | 6 | 2 | 8 | 234 | 214 | 437 | 2.05e-152 | 440 |
| MsG0780040487.01.T02 | G7KTI4 | 95.946 | 222 | 5 | 1 | 1 | 222 | 220 | 437 | 1.20e-149 | 432 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004142.01 | MsG0780040487.01 | 0.821960 | 3.057350e-53 | 2.524226e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040487.01.T01 | MTR_7g091450 | 95.175 | 228 | 6 | 2 | 8 | 234 | 214 | 437 | 5.20e-156 | 440 |
| MsG0780040487.01.T02 | MTR_7g091450 | 95.946 | 222 | 5 | 1 | 1 | 222 | 220 | 437 | 3.03e-153 | 432 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040487.01.T01 | AT3G57400 | 54.741 | 232 | 82 | 6 | 21 | 234 | 248 | 474 | 5.16e-70 | 222 |
| MsG0780040487.01.T01 | AT5G52500 | 53.297 | 182 | 74 | 4 | 11 | 191 | 190 | 361 | 6.56e-60 | 192 |
| MsG0780040487.01.T02 | AT3G57400 | 54.741 | 232 | 82 | 6 | 9 | 222 | 248 | 474 | 1.99e-70 | 223 |
| MsG0780040487.01.T02 | AT5G52500 | 55.758 | 165 | 64 | 3 | 16 | 179 | 205 | 361 | 3.25e-58 | 188 |
Find 43 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTCATTATCAGGCTTTC+TGG | 0.141001 | 7:+78307430 | MsG0780040487.01.T01:CDS |
| TTGAAGAGCAAAACCGATTC+TGG | 0.205334 | 7:-78307599 | None:intergenic |
| TACCCCAAGGGTTCTGAAAC+TGG | 0.257885 | 7:-78307313 | None:intergenic |
| GATCCAGAAAACTCATTATC+AGG | 0.334398 | 7:+78307422 | MsG0780040487.01.T01:CDS |
| CGATTCTTGACTCGGTTAAA+CGG | 0.344065 | 7:-78307572 | None:intergenic |
| GTTCTACCCGCCGTTGTCAT+TGG | 0.355085 | 7:+78307662 | MsG0780040487.01.T01:CDS |
| GATTCTTGACTCGGTTAAAC+GGG | 0.406204 | 7:-78307571 | None:intergenic |
| ACAACTGCTTCACTGAGTTA+TGG | 0.407393 | 7:+78307368 | MsG0780040487.01.T01:CDS |
| CAACTGCTTCACTGAGTTAT+GGG | 0.409654 | 7:+78307369 | MsG0780040487.01.T01:CDS |
| CCATTGAGTGAACTCACTTC+AGG | 0.447724 | 7:-78307866 | None:intergenic |
| CTGGACTCCGATTCTTGACT+CGG | 0.452821 | 7:-78307580 | None:intergenic |
| CACCCAGTTTCAGAACCCTT+GGG | 0.456391 | 7:+78307310 | MsG0780040487.01.T01:CDS |
| CCACCCAGTTTCAGAACCCT+TGG | 0.457191 | 7:+78307309 | MsG0780040487.01.T01:CDS |
| GCCCACCGTCGTAGACTAAA+CGG | 0.474798 | 7:-78307461 | None:intergenic |
| CGTTTAACCGAGTCAAGAAT+CGG | 0.480938 | 7:+78307573 | MsG0780040487.01.T01:CDS |
| TAAGAAACAAATTTGTTCGT+CGG | 0.497505 | 7:+78307946 | MsG0780040487.01.T01:CDS |
| GCGGTGCTCGCGGTGTCCGA+CGG | 0.498086 | 7:+78307684 | MsG0780040487.01.T01:CDS |
| CTGAAGTGAGTTCACTCAAT+GGG | 0.511702 | 7:+78307867 | MsG0780040487.01.T01:CDS |
| TTCCGTTTAGTCTACGACGG+TGG | 0.520250 | 7:+78307459 | MsG0780040487.01.T01:CDS |
| GCATGTTACCCAATGCTACT+TGG | 0.525319 | 7:+78307482 | MsG0780040487.01.T01:CDS |
| CAAGAATCGGAGTCCAGAAT+CGG | 0.531864 | 7:+78307586 | MsG0780040487.01.T01:CDS |
| CTACCCGCCGTTGTCATTGG+CGG | 0.536791 | 7:+78307665 | MsG0780040487.01.T01:CDS |
| ACGGTTGTCTAACGCAGTCA+TGG | 0.539525 | 7:+78307703 | MsG0780040487.01.T01:CDS |
| TTTCGTAATTTATTACCCCA+AGG | 0.544898 | 7:-78307326 | None:intergenic |
| GCACCGCCAATGACAACGGC+GGG | 0.546801 | 7:-78307668 | None:intergenic |
| CGAACACGACCAAGTAGCAT+TGG | 0.547415 | 7:-78307491 | None:intergenic |
| ACCCCAAGGGTTCTGAAACT+GGG | 0.548893 | 7:-78307312 | None:intergenic |
| TTCGTAATTTATTACCCCAA+GGG | 0.553447 | 7:-78307325 | None:intergenic |
| GAACACGACCAAGTAGCATT+GGG | 0.558914 | 7:-78307490 | None:intergenic |
| TCCGTTTAGTCTACGACGGT+GGG | 0.567770 | 7:+78307460 | MsG0780040487.01.T01:CDS |
| CCTGAAGTGAGTTCACTCAA+TGG | 0.579268 | 7:+78307866 | MsG0780040487.01.T01:CDS |
| TTCGTGCTGCGACTCAGCAT+AGG | 0.580434 | 7:+78307735 | MsG0780040487.01.T01:CDS |
| GCGAGCACCGCCAATGACAA+CGG | 0.581722 | 7:-78307672 | None:intergenic |
| GAAACAAATTTGTTCGTCGG+TGG | 0.590290 | 7:+78307949 | MsG0780040487.01.T01:CDS |
| GTTGTCATTGGCGGTGCTCG+CGG | 0.609416 | 7:+78307674 | MsG0780040487.01.T01:CDS |
| TGCTTCACTGAGTTATGGGT+CGG | 0.617966 | 7:+78307373 | MsG0780040487.01.T01:CDS |
| GTCGTAGACTAAACGGAACT+CGG | 0.634835 | 7:-78307454 | None:intergenic |
| ACCCAGTTTCAGAACCCTTG+GGG | 0.687588 | 7:+78307311 | MsG0780040487.01.T01:CDS |
| TGACTGCGTTAGACAACCGT+CGG | 0.696424 | 7:-78307700 | None:intergenic |
| AGCACCGCCAATGACAACGG+CGG | 0.700363 | 7:-78307669 | None:intergenic |
| CCAAGGGTTCTGAAACTGGG+TGG | 0.719462 | 7:-78307309 | None:intergenic |
| GAGTTCCGTTTAGTCTACGA+CGG | 0.724900 | 7:+78307456 | MsG0780040487.01.T01:CDS |
| AATCATGCAATAATTCAACG+TGG | 0.815559 | 7:+78307893 | MsG0780040487.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAACAATTCTCATTCTTC+GGG | + | Chr7:78307547-78307566 | MsG0780040487.01.T01:CDS | 25.0% |
| ! | CAAAAACAATTCTCATTCTT+CGG | + | Chr7:78307546-78307565 | MsG0780040487.01.T01:CDS | 25.0% |
| !! | TAAGAAACAAATTTGTTCGT+CGG | + | Chr7:78307946-78307965 | MsG0780040487.01.T01:CDS | 25.0% |
| AATCATGCAATAATTCAACG+TGG | + | Chr7:78307893-78307912 | MsG0780040487.01.T01:CDS | 30.0% | |
| TTCGTAATTTATTACCCCAA+GGG | - | Chr7:78307328-78307347 | None:intergenic | 30.0% | |
| TTTCGTAATTTATTACCCCA+AGG | - | Chr7:78307329-78307348 | None:intergenic | 30.0% | |
| AAACTCATTATCAGGCTTTC+TGG | + | Chr7:78307430-78307449 | MsG0780040487.01.T01:CDS | 35.0% | |
| GAAAAACTCGGTTACTGAAT+CGG | + | Chr7:78307763-78307782 | MsG0780040487.01.T01:CDS | 35.0% | |
| GATCCAGAAAACTCATTATC+AGG | + | Chr7:78307422-78307441 | MsG0780040487.01.T01:CDS | 35.0% | |
| GCATAGGAAGAAGAAAAACT+CGG | + | Chr7:78307751-78307770 | MsG0780040487.01.T01:CDS | 35.0% | |
| ! | GTTTTGCTCTTCAACAAACA+CGG | + | Chr7:78307608-78307627 | MsG0780040487.01.T01:CDS | 35.0% |
| !! | AAGCCTGATAATGAGTTTTC+TGG | - | Chr7:78307428-78307447 | None:intergenic | 35.0% |
| ACAACTGCTTCACTGAGTTA+TGG | + | Chr7:78307368-78307387 | MsG0780040487.01.T01:CDS | 40.0% | |
| CAACTGCTTCACTGAGTTAT+GGG | + | Chr7:78307369-78307388 | MsG0780040487.01.T01:CDS | 40.0% | |
| CGTTTAACCGAGTCAAGAAT+CGG | + | Chr7:78307573-78307592 | MsG0780040487.01.T01:CDS | 40.0% | |
| CTGAAGTGAGTTCACTCAAT+GGG | + | Chr7:78307867-78307886 | MsG0780040487.01.T01:CDS | 40.0% | |
| ! | GAAACAAATTTGTTCGTCGG+TGG | + | Chr7:78307949-78307968 | MsG0780040487.01.T01:CDS | 40.0% |
| ! | TTGAAGAGCAAAACCGATTC+TGG | - | Chr7:78307602-78307621 | None:intergenic | 40.0% |
| !! | CGATTCTTGACTCGGTTAAA+CGG | - | Chr7:78307575-78307594 | None:intergenic | 40.0% |
| !! | GATTCTTGACTCGGTTAAAC+GGG | - | Chr7:78307574-78307593 | None:intergenic | 40.0% |
| !! | GGTACTAAGTTTTGACGCAA+CGG | + | Chr7:78307394-78307413 | MsG0780040487.01.T01:CDS | 40.0% |
| CAAGAATCGGAGTCCAGAAT+CGG | + | Chr7:78307586-78307605 | MsG0780040487.01.T01:CDS | 45.0% | |
| CCATTGAGTGAACTCACTTC+AGG | - | Chr7:78307869-78307888 | None:intergenic | 45.0% | |
| CCTGAAGTGAGTTCACTCAA+TGG | + | Chr7:78307866-78307885 | MsG0780040487.01.T01:CDS | 45.0% | |
| GAACACGACCAAGTAGCATT+GGG | - | Chr7:78307493-78307512 | None:intergenic | 45.0% | |
| GAGTTCCGTTTAGTCTACGA+CGG | + | Chr7:78307456-78307475 | MsG0780040487.01.T01:CDS | 45.0% | |
| GCATGTTACCCAATGCTACT+TGG | + | Chr7:78307482-78307501 | MsG0780040487.01.T01:CDS | 45.0% | |
| GTCGTAGACTAAACGGAACT+CGG | - | Chr7:78307457-78307476 | None:intergenic | 45.0% | |
| TGCTTCACTGAGTTATGGGT+CGG | + | Chr7:78307373-78307392 | MsG0780040487.01.T01:CDS | 45.0% | |
| ACCCAGTTTCAGAACCCTTG+GGG | + | Chr7:78307311-78307330 | MsG0780040487.01.T01:CDS | 50.0% | |
| ACGGTTGTCTAACGCAGTCA+TGG | + | Chr7:78307703-78307722 | MsG0780040487.01.T01:CDS | 50.0% | |
| CACCCAGTTTCAGAACCCTT+GGG | + | Chr7:78307310-78307329 | MsG0780040487.01.T01:CDS | 50.0% | |
| CGAACACGACCAAGTAGCAT+TGG | - | Chr7:78307494-78307513 | None:intergenic | 50.0% | |
| CTGGACTCCGATTCTTGACT+CGG | - | Chr7:78307583-78307602 | None:intergenic | 50.0% | |
| TCCGTTTAGTCTACGACGGT+GGG | + | Chr7:78307460-78307479 | MsG0780040487.01.T01:CDS | 50.0% | |
| TGACTGCGTTAGACAACCGT+CGG | - | Chr7:78307703-78307722 | None:intergenic | 50.0% | |
| TTCCGTTTAGTCTACGACGG+TGG | + | Chr7:78307459-78307478 | MsG0780040487.01.T01:CDS | 50.0% | |
| !! | ACCCCAAGGGTTCTGAAACT+GGG | - | Chr7:78307315-78307334 | None:intergenic | 50.0% |
| !! | TACCCCAAGGGTTCTGAAAC+TGG | - | Chr7:78307316-78307335 | None:intergenic | 50.0% |
| CCACCCAGTTTCAGAACCCT+TGG | + | Chr7:78307309-78307328 | MsG0780040487.01.T01:CDS | 55.0% | |
| GCCCACCGTCGTAGACTAAA+CGG | - | Chr7:78307464-78307483 | None:intergenic | 55.0% | |
| GTTCTACCCGCCGTTGTCAT+TGG | + | Chr7:78307662-78307681 | MsG0780040487.01.T01:CDS | 55.0% | |
| TTCGTGCTGCGACTCAGCAT+AGG | + | Chr7:78307735-78307754 | MsG0780040487.01.T01:CDS | 55.0% | |
| !! | CCAAGGGTTCTGAAACTGGG+TGG | - | Chr7:78307312-78307331 | None:intergenic | 55.0% |
| AGCACCGCCAATGACAACGG+CGG | - | Chr7:78307672-78307691 | None:intergenic | 60.0% | |
| CTACCCGCCGTTGTCATTGG+CGG | + | Chr7:78307665-78307684 | MsG0780040487.01.T01:CDS | 60.0% | |
| GTTGTCATTGGCGGTGCTCG+CGG | + | Chr7:78307674-78307693 | MsG0780040487.01.T01:CDS | 60.0% | |
| ! | GCGAGCACCGCCAATGACAA+CGG | - | Chr7:78307675-78307694 | None:intergenic | 60.0% |
| GCACCGCCAATGACAACGGC+GGG | - | Chr7:78307671-78307690 | None:intergenic | 65.0% | |
| GCGGTGCTCGCGGTGTCCGA+CGG | + | Chr7:78307684-78307703 | MsG0780040487.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 78307299 | 78308003 | 78307299 | ID=MsG0780040487.01;Name=MsG0780040487.01 |
| Chr7 | mRNA | 78307299 | 78308003 | 78307299 | ID=MsG0780040487.01.T01;Parent=MsG0780040487.01;Name=MsG0780040487.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|234 |
| Chr7 | exon | 78307299 | 78308003 | 78307299 | ID=MsG0780040487.01.T01:exon:16330;Parent=MsG0780040487.01.T01 |
| Chr7 | CDS | 78307299 | 78308003 | 78307299 | ID=MsG0780040487.01.T01:cds;Parent=MsG0780040487.01.T01 |
| Chr7 | mRNA | 78307335 | 78308003 | 78307335 | ID=MsG0780040487.01.T02;Parent=MsG0780040487.01;Name=MsG0780040487.01.T02;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|222 |
| Chr7 | exon | 78307335 | 78308003 | 78307335 | ID=MsG0780040487.01.T02:exon:16331;Parent=MsG0780040487.01.T02 |
| Chr7 | CDS | 78307335 | 78308003 | 78307335 | ID=MsG0780040487.01.T02:cds;Parent=MsG0780040487.01.T02 |
| Gene Sequence |
| Protein sequence |
