Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040606.01.T01 | XP_003625307.2 | 96.61 | 118 | 4 | 0 | 1 | 118 | 1 | 118 | 9.35E-72 | 242 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040606.01.T01 | F4J8L3 | 64.407 | 118 | 38 | 1 | 1 | 118 | 1 | 114 | 8.64E-34 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040606.01.T01 | G7KWB5 | 96.610 | 118 | 4 | 0 | 1 | 118 | 1 | 118 | 4.47e-72 | 242 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004520.01 | MsG0780040606.01 | 0.804965 | 1.677141e-49 | 8.766068e-47 |
| MsG0780040606.01 | MsG0880046524.01 | 0.811597 | 6.464059e-51 | 4.017943e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040606.01.T01 | MTR_7g093720 | 96.610 | 118 | 4 | 0 | 1 | 118 | 1 | 118 | 1.13e-75 | 242 |
| MsG0780040606.01.T01 | MTR_4g071210 | 41.509 | 106 | 57 | 1 | 18 | 118 | 29 | 134 | 3.03e-18 | 80.1 |
| MsG0780040606.01.T01 | MTR_4g071210 | 41.509 | 106 | 57 | 1 | 18 | 118 | 29 | 134 | 3.06e-18 | 80.1 |
| MsG0780040606.01.T01 | MTR_3g117330 | 54.545 | 55 | 25 | 0 | 64 | 118 | 90 | 144 | 4.18e-18 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040606.01.T01 | AT3G12020 | 64.407 | 118 | 38 | 1 | 1 | 118 | 1 | 114 | 7.59e-35 | 127 |
| MsG0780040606.01.T01 | AT3G12020 | 64.407 | 118 | 38 | 1 | 1 | 118 | 1 | 114 | 7.59e-35 | 127 |
| MsG0780040606.01.T01 | AT3G12020 | 64.407 | 118 | 38 | 1 | 1 | 118 | 1 | 114 | 8.81e-35 | 127 |
| MsG0780040606.01.T01 | AT3G12020 | 64.407 | 118 | 38 | 1 | 1 | 118 | 1 | 114 | 8.81e-35 | 127 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 3.56e-26 | 102 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 3.56e-26 | 102 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 4.00e-26 | 102 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 4.00e-26 | 102 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 8.03e-26 | 101 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 8.03e-26 | 101 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 8.03e-26 | 101 |
| MsG0780040606.01.T01 | AT5G06670 | 62.712 | 118 | 37 | 2 | 1 | 118 | 1 | 111 | 8.05e-26 | 101 |
| MsG0780040606.01.T01 | AT1G21730 | 66.000 | 50 | 17 | 0 | 69 | 118 | 71 | 120 | 6.22e-21 | 87.8 |
| MsG0780040606.01.T01 | AT4G39050 | 60.417 | 48 | 19 | 0 | 71 | 118 | 96 | 143 | 1.82e-16 | 75.1 |
| MsG0780040606.01.T01 | AT2G21380 | 56.250 | 48 | 21 | 0 | 71 | 118 | 102 | 149 | 5.10e-15 | 70.9 |
| MsG0780040606.01.T01 | AT2G21380 | 56.250 | 48 | 21 | 0 | 71 | 118 | 102 | 149 | 5.10e-15 | 70.9 |
Find 31 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCCAACGGCACAGCTTC+TGG | 0.252396 | 7:+79884965 | None:intergenic |
| TCTCTCACACTTTCCACTTC+TGG | 0.281662 | 7:-79885389 | MsG0780040606.01.T01:five_prime_UTR |
| CAAGGCTCATATTTGTTGAT+AGG | 0.294958 | 7:-79884684 | MsG0780040606.01.T01:CDS |
| CCGTAAGCATAAGCTATAGA+AGG | 0.372184 | 7:+79884717 | None:intergenic |
| GGACCGAAATCAAAACGATT+TGG | 0.378030 | 7:-79885127 | MsG0780040606.01.T01:CDS |
| TGTAGAAGATCGCGTTGAAA+TGG | 0.408439 | 7:+79885351 | None:intergenic |
| TCAAAACGATTTGGATCGAT+TGG | 0.414281 | 7:-79885118 | MsG0780040606.01.T01:CDS |
| TAACTGTCACTGTCAGGTTT+CGG | 0.415484 | 7:-79884922 | MsG0780040606.01.T01:CDS |
| CCTTCTATAGCTTATGCTTA+CGG | 0.441558 | 7:-79884717 | MsG0780040606.01.T01:CDS |
| GGGCGAACTCGGAGCATCAT+TGG | 0.443297 | 7:+79885027 | None:intergenic |
| AGAATGTAACTGTCACTGTC+AGG | 0.492863 | 7:-79884928 | MsG0780040606.01.T01:CDS |
| GACGAAGACGCGGGCGAACT+CGG | 0.497365 | 7:+79885016 | None:intergenic |
| AAACAAAACACTTACTTCAA+AGG | 0.518766 | 7:+79884899 | None:intergenic |
| AAACGATTTGGATCGATTGG+AGG | 0.520737 | 7:-79885115 | MsG0780040606.01.T01:CDS |
| GTGTGAATGGCGTCGAAACA+AGG | 0.560798 | 7:-79885148 | MsG0780040606.01.T01:exon |
| GAGATTGCTTGGTATGCAGA+TGG | 0.562206 | 7:-79884768 | MsG0780040606.01.T01:CDS |
| GACGAAGTTGTTGAAGAAGA+AGG | 0.572326 | 7:+79885073 | None:intergenic |
| GTCATGGCGAGGAGATTGCT+TGG | 0.575174 | 7:-79884779 | MsG0780040606.01.T01:CDS |
| TCGCCATGACGAATTTCCCT+CGG | 0.585204 | 7:+79884792 | None:intergenic |
| GGATCGATTGGAGGAACGAA+AGG | 0.586015 | 7:-79885106 | MsG0780040606.01.T01:CDS |
| TTATCCAGAAGCTGTGCCGT+TGG | 0.587474 | 7:-79884969 | MsG0780040606.01.T01:CDS |
| CGAACTCGGAGCATCATTGG+AGG | 0.597760 | 7:+79885030 | None:intergenic |
| ACTCCGCACTGACGAAGACG+CGG | 0.613192 | 7:+79885006 | None:intergenic |
| GAAAGAAACAGATCCAGAAG+TGG | 0.620539 | 7:+79885376 | None:intergenic |
| CTCCGCACTGACGAAGACGC+GGG | 0.631526 | 7:+79885007 | None:intergenic |
| GCTTCTGGATAAAACTGCTG+CGG | 0.640289 | 7:+79884980 | None:intergenic |
| GCTTTGACGAGAAGTGTGAA+TGG | 0.655384 | 7:-79885161 | MsG0780040606.01.T01:exon |
| AGTCCGAGGGAAATTCGTCA+TGG | 0.659074 | 7:-79884795 | MsG0780040606.01.T01:CDS |
| GAGGGAAATTCGTCATGGCG+AGG | 0.661625 | 7:-79884790 | MsG0780040606.01.T01:CDS |
| CGCCCGCGTCTTCGTCAGTG+CGG | 0.678236 | 7:-79885009 | MsG0780040606.01.T01:CDS |
| TTATGTGTGTGACAGTCCGA+GGG | 0.698404 | 7:-79884808 | MsG0780040606.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACAAAACACTTACTTCAA+AGG | + | Chr7:79885261-79885280 | None:intergenic | 25.0% |
| TGCGTAAGATAAGATAAGAA+TGG | + | Chr7:79884867-79884886 | None:intergenic | 30.0% | |
| ! | TGTTGATGTTAATTTGATCG+AGG | - | Chr7:79885317-79885336 | MsG0780040606.01.T01:intron | 30.0% |
| ! | TTCTCTGATTCTTCATTTTC+AGG | - | Chr7:79884833-79884852 | MsG0780040606.01.T01:intron | 30.0% |
| !! | TTAGTCTTTTGAGAATCCAA+CGG | + | Chr7:79885207-79885226 | None:intergenic | 30.0% |
| !!! | GATCCAAATCGTTTTGATTT+CGG | + | Chr7:79885036-79885055 | None:intergenic | 30.0% |
| CCTTCTATAGCTTATGCTTA+CGG | - | Chr7:79885440-79885459 | MsG0780040606.01.T01:five_prime_UTR | 35.0% | |
| GCTTACGGTAAAAATTTGCA+AGG | - | Chr7:79885455-79885474 | MsG0780040606.01.T01:five_prime_UTR | 35.0% | |
| ! | ATGTTAATTTGATCGAGGTG+TGG | - | Chr7:79885322-79885341 | MsG0780040606.01.T01:intron | 35.0% |
| ! | CAAGGCTCATATTTGTTGAT+AGG | - | Chr7:79885473-79885492 | MsG0780040606.01.T01:five_prime_UTR | 35.0% |
| ! | TCAAAACGATTTGGATCGAT+TGG | - | Chr7:79885039-79885058 | MsG0780040606.01.T01:CDS | 35.0% |
| AGAATGTAACTGTCACTGTC+AGG | - | Chr7:79885229-79885248 | MsG0780040606.01.T01:intron | 40.0% | |
| CCGTAAGCATAAGCTATAGA+AGG | + | Chr7:79885443-79885462 | None:intergenic | 40.0% | |
| GAAAGAAACAGATCCAGAAG+TGG | + | Chr7:79884784-79884803 | None:intergenic | 40.0% | |
| GACGAAGTTGTTGAAGAAGA+AGG | + | Chr7:79885087-79885106 | None:intergenic | 40.0% | |
| GGACCGAAATCAAAACGATT+TGG | - | Chr7:79885030-79885049 | MsG0780040606.01.T01:CDS | 40.0% | |
| TAACTGTCACTGTCAGGTTT+CGG | - | Chr7:79885235-79885254 | MsG0780040606.01.T01:intron | 40.0% | |
| TGTAGAAGATCGCGTTGAAA+TGG | + | Chr7:79884809-79884828 | None:intergenic | 40.0% | |
| ! | AAACGATTTGGATCGATTGG+AGG | - | Chr7:79885042-79885061 | MsG0780040606.01.T01:CDS | 40.0% |
| GAGATTGCTTGGTATGCAGA+TGG | - | Chr7:79885389-79885408 | MsG0780040606.01.T01:five_prime_UTR | 45.0% | |
| GCTTCTGGATAAAACTGCTG+CGG | + | Chr7:79885180-79885199 | None:intergenic | 45.0% | |
| TCTCTCACACTTTCCACTTC+TGG | - | Chr7:79884768-79884787 | MsG0780040606.01.T01:CDS | 45.0% | |
| TTATGTGTGTGACAGTCCGA+GGG | - | Chr7:79885349-79885368 | MsG0780040606.01.T01:five_prime_UTR | 45.0% | |
| ! | GCTTTGACGAGAAGTGTGAA+TGG | - | Chr7:79884996-79885015 | MsG0780040606.01.T01:CDS | 45.0% |
| AGTCCGAGGGAAATTCGTCA+TGG | - | Chr7:79885362-79885381 | MsG0780040606.01.T01:five_prime_UTR | 50.0% | |
| GGATCGATTGGAGGAACGAA+AGG | - | Chr7:79885051-79885070 | MsG0780040606.01.T01:CDS | 50.0% | |
| GTTATGTGTGTGACAGTCCG+AGG | - | Chr7:79885348-79885367 | MsG0780040606.01.T01:five_prime_UTR | 50.0% | |
| TCGCCATGACGAATTTCCCT+CGG | + | Chr7:79885368-79885387 | None:intergenic | 50.0% | |
| TTATCCAGAAGCTGTGCCGT+TGG | - | Chr7:79885188-79885207 | MsG0780040606.01.T01:intron | 50.0% | |
| ! | GTGTGAATGGCGTCGAAACA+AGG | - | Chr7:79885009-79885028 | MsG0780040606.01.T01:CDS | 50.0% |
| GAATCCAACGGCACAGCTTC+TGG | + | Chr7:79885195-79885214 | None:intergenic | 55.0% | |
| GAGGGAAATTCGTCATGGCG+AGG | - | Chr7:79885367-79885386 | MsG0780040606.01.T01:five_prime_UTR | 55.0% | |
| GTCATGGCGAGGAGATTGCT+TGG | - | Chr7:79885378-79885397 | MsG0780040606.01.T01:five_prime_UTR | 55.0% | |
| ! | CGAACTCGGAGCATCATTGG+AGG | + | Chr7:79885130-79885149 | None:intergenic | 55.0% |
| ! | ACTCCGCACTGACGAAGACG+CGG | + | Chr7:79885154-79885173 | None:intergenic | 60.0% |
| ! | GGGCGAACTCGGAGCATCAT+TGG | + | Chr7:79885133-79885152 | None:intergenic | 60.0% |
| GACGAAGACGCGGGCGAACT+CGG | + | Chr7:79885144-79885163 | None:intergenic | 65.0% | |
| ! | CTCCGCACTGACGAAGACGC+GGG | + | Chr7:79885153-79885172 | None:intergenic | 65.0% |
| CGCCCGCGTCTTCGTCAGTG+CGG | - | Chr7:79885148-79885167 | MsG0780040606.01.T01:exon | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 79884668 | 79885511 | 79884668 | ID=MsG0780040606.01;Name=MsG0780040606.01 |
| Chr7 | mRNA | 79884668 | 79885511 | 79884668 | ID=MsG0780040606.01.T01;Parent=MsG0780040606.01;Name=MsG0780040606.01.T01;_AED=0.49;_eAED=0.52;_QI=205|1|0.66|1|0.5|0.33|3|0|132 |
| Chr7 | exon | 79884668 | 79884815 | 79884668 | ID=MsG0780040606.01.T01:exon:605;Parent=MsG0780040606.01.T01 |
| Chr7 | exon | 79884914 | 79885182 | 79884914 | ID=MsG0780040606.01.T01:exon:604;Parent=MsG0780040606.01.T01 |
| Chr7 | exon | 79885325 | 79885511 | 79885325 | ID=MsG0780040606.01.T01:exon:603;Parent=MsG0780040606.01.T01 |
| Chr7 | five_prime_UTR | 79885325 | 79885511 | 79885325 | ID=MsG0780040606.01.T01:five_prime_utr;Parent=MsG0780040606.01.T01 |
| Chr7 | five_prime_UTR | 79885165 | 79885182 | 79885165 | ID=MsG0780040606.01.T01:five_prime_utr;Parent=MsG0780040606.01.T01 |
| Chr7 | CDS | 79884914 | 79885164 | 79884914 | ID=MsG0780040606.01.T01:cds;Parent=MsG0780040606.01.T01 |
| Chr7 | CDS | 79884668 | 79884815 | 79884668 | ID=MsG0780040606.01.T01:cds;Parent=MsG0780040606.01.T01 |
| Gene Sequence |
| Protein sequence |
