Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041090.01.T01 | AFK43856.1 | 95.513 | 156 | 4 | 2 | 1 | 156 | 73 | 225 | 1.04E-100 | 304 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041090.01.T01 | Q9SJR5 | 74.797 | 123 | 18 | 3 | 3 | 117 | 73 | 190 | 3.86E-55 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041090.01.T01 | I3SUB4 | 95.513 | 156 | 4 | 2 | 1 | 156 | 73 | 225 | 4.95e-101 | 304 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780041090.01.T01 | TF | GRF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780039446.01 | MsG0780041090.01 | 0.824301 | 8.692409e-54 | 7.668620e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041090.01.T01 | MTR_7g104050 | 95.513 | 156 | 4 | 2 | 1 | 156 | 73 | 225 | 1.26e-104 | 303 |
| MsG0780041090.01.T01 | MTR_7g104050 | 95.513 | 156 | 4 | 2 | 1 | 156 | 73 | 225 | 1.31e-104 | 304 |
| MsG0780041090.01.T01 | MTR_0001s0490 | 60.714 | 112 | 41 | 3 | 7 | 117 | 26 | 135 | 1.20e-39 | 136 |
| MsG0780041090.01.T01 | MTR_4g125490 | 51.471 | 136 | 51 | 4 | 7 | 135 | 10 | 137 | 5.11e-37 | 131 |
| MsG0780041090.01.T01 | MTR_8g020560 | 57.658 | 111 | 43 | 3 | 7 | 115 | 12 | 120 | 1.54e-35 | 126 |
| MsG0780041090.01.T01 | MTR_5g027030 | 61.111 | 108 | 30 | 4 | 7 | 114 | 17 | 112 | 2.10e-35 | 125 |
| MsG0780041090.01.T01 | MTR_2g041430 | 57.391 | 115 | 41 | 3 | 7 | 115 | 8 | 120 | 3.14e-35 | 125 |
| MsG0780041090.01.T01 | MTR_2g041430 | 57.391 | 115 | 41 | 3 | 7 | 115 | 8 | 120 | 4.10e-35 | 125 |
| MsG0780041090.01.T01 | MTR_2g041430 | 57.391 | 115 | 41 | 3 | 7 | 115 | 8 | 120 | 4.23e-35 | 125 |
| MsG0780041090.01.T01 | MTR_1g017490 | 55.357 | 112 | 46 | 2 | 7 | 114 | 12 | 123 | 5.68e-33 | 122 |
| MsG0780041090.01.T01 | MTR_1g017490 | 55.357 | 112 | 46 | 2 | 7 | 114 | 145 | 256 | 7.12e-33 | 122 |
| MsG0780041090.01.T01 | MTR_3g092330 | 45.217 | 115 | 37 | 2 | 7 | 117 | 114 | 206 | 2.36e-26 | 103 |
| MsG0780041090.01.T01 | MTR_8g035910 | 40.252 | 159 | 60 | 7 | 7 | 154 | 50 | 184 | 2.55e-23 | 94.7 |
| MsG0780041090.01.T01 | MTR_8g035910 | 60.784 | 51 | 20 | 0 | 70 | 120 | 297 | 347 | 1.95e-15 | 72.8 |
| MsG0780041090.01.T01 | MTR_8g035640 | 58.000 | 50 | 21 | 0 | 70 | 119 | 51 | 100 | 2.42e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041090.01.T01 | AT2G36400 | 74.797 | 123 | 18 | 3 | 3 | 117 | 73 | 190 | 3.94e-56 | 181 |
| MsG0780041090.01.T01 | AT3G52910 | 72.358 | 123 | 20 | 3 | 1 | 114 | 77 | 194 | 9.31e-56 | 179 |
| MsG0780041090.01.T01 | AT4G37740 | 45.395 | 152 | 67 | 5 | 2 | 147 | 160 | 301 | 1.55e-33 | 124 |
| MsG0780041090.01.T01 | AT3G13960 | 53.636 | 110 | 47 | 3 | 7 | 114 | 17 | 124 | 4.55e-30 | 112 |
| MsG0780041090.01.T01 | AT2G22840 | 54.701 | 117 | 43 | 4 | 2 | 114 | 129 | 239 | 1.13e-28 | 110 |
| MsG0780041090.01.T01 | AT5G53660 | 47.899 | 119 | 43 | 3 | 7 | 123 | 60 | 161 | 2.05e-26 | 102 |
| MsG0780041090.01.T01 | AT4G24150 | 46.957 | 115 | 57 | 2 | 4 | 114 | 109 | 223 | 1.24e-25 | 101 |
| MsG0780041090.01.T01 | AT4G24150 | 46.957 | 115 | 57 | 2 | 4 | 114 | 147 | 261 | 2.13e-25 | 100 |
| MsG0780041090.01.T01 | AT2G06200 | 49.573 | 117 | 48 | 4 | 7 | 112 | 46 | 162 | 7.19e-25 | 97.1 |
| MsG0780041090.01.T01 | AT2G06200 | 49.573 | 117 | 48 | 4 | 7 | 112 | 7 | 123 | 8.41e-25 | 96.3 |
| MsG0780041090.01.T01 | AT2G45480 | 48.039 | 102 | 53 | 0 | 10 | 111 | 28 | 129 | 4.95e-23 | 92.8 |
| MsG0780041090.01.T01 | AT2G45480 | 48.039 | 102 | 53 | 0 | 10 | 111 | 28 | 129 | 2.94e-22 | 92.0 |
| MsG0780041090.01.T01 | AT2G45480 | 45.977 | 87 | 31 | 3 | 53 | 132 | 286 | 363 | 1.26e-18 | 81.6 |
| MsG0780041090.01.T01 | AT2G45480 | 48.039 | 102 | 53 | 0 | 10 | 111 | 26 | 127 | 3.00e-22 | 92.0 |
| MsG0780041090.01.T01 | AT2G45480 | 45.977 | 87 | 31 | 3 | 53 | 132 | 284 | 361 | 1.35e-18 | 81.6 |
| MsG0780041090.01.T01 | AT4G24150 | 40.000 | 140 | 55 | 3 | 4 | 114 | 147 | 286 | 3.38e-22 | 92.0 |
| MsG0780041090.01.T01 | AT2G06200 | 71.111 | 45 | 11 | 1 | 70 | 112 | 7 | 51 | 1.25e-16 | 73.2 |
Find 40 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGCAAGAACTTGAGTTAC+AGG | 0.267624 | 7:+86436034 | MsG0780041090.01.T01:CDS |
| AGTGTTGCAAAGTGGGTATT+GGG | 0.272050 | 7:+86436568 | MsG0780041090.01.T01:intron |
| TGATAATGATGAAGAAAATA+AGG | 0.326826 | 7:-86436143 | None:intergenic |
| TTGATAGGTTGAAGAAGTTC+AGG | 0.344449 | 7:-86436098 | None:intergenic |
| ATCCAGAGCCAGGTCGTTGC+CGG | 0.359541 | 7:+86436606 | MsG0780041090.01.T01:CDS |
| CTCCGGCAACGACCTGGCTC+TGG | 0.400600 | 7:-86436608 | None:intergenic |
| TGTGCGAGGGATGTGGTGGC+TGG | 0.410154 | 7:+86436653 | MsG0780041090.01.T01:CDS |
| GCCTCTGAGATAAGCTGAAA+TGG | 0.420696 | 7:-86436016 | None:intergenic |
| TAGTGTTGCAAAGTGGGTAT+TGG | 0.440707 | 7:+86436567 | MsG0780041090.01.T01:intron |
| GGAGCAATGGATCCAGAGCC+AGG | 0.451345 | 7:+86436596 | MsG0780041090.01.T01:CDS |
| TACATTATTAGGAATGGGAA+TGG | 0.465443 | 7:+86435989 | None:intergenic |
| CAAAGTGGGTATTGGGGAAG+AGG | 0.496611 | 7:+86436575 | MsG0780041090.01.T01:CDS |
| GTGGCGGTGTGCGAGGGATG+TGG | 0.497767 | 7:+86436646 | MsG0780041090.01.T01:CDS |
| GTTGGTGTGGGAAGTTCCAC+AGG | 0.502197 | 7:-86436728 | None:intergenic |
| TGATTAAAGTGAAGATCAAA+AGG | 0.508625 | 7:-86436836 | None:intergenic |
| GTTCCACAGGCTTTCTTGAA+CGG | 0.512655 | 7:-86436715 | None:intergenic |
| TCTGTTCTCCGGCAACGACC+TGG | 0.516750 | 7:-86436614 | None:intergenic |
| ACATTATTAGGAATGGGAAT+GGG | 0.520045 | 7:+86435990 | None:intergenic |
| TTTGATATTTAGATACATGT+TGG | 0.524543 | 7:+86436058 | MsG0780041090.01.T01:CDS |
| TTGATCTTCACTTTAATCAA+CGG | 0.532715 | 7:+86436840 | MsG0780041090.01.T01:CDS |
| TTTGATTAGTGTTGCAAAGT+GGG | 0.534160 | 7:+86436561 | MsG0780041090.01.T01:intron |
| GTGTTGCAAAGTGGGTATTG+GGG | 0.548784 | 7:+86436569 | MsG0780041090.01.T01:intron |
| CTTCCCACACCAACTAGTAA+TGG | 0.551902 | 7:+86436737 | MsG0780041090.01.T01:CDS |
| ATCAAAAGGAGATTTGAATG+AGG | 0.554725 | 7:-86436822 | None:intergenic |
| ACCGCCATTACTAGTTGGTG+TGG | 0.561256 | 7:-86436741 | None:intergenic |
| ATATTTAGATACATGTTGGT+TGG | 0.568638 | 7:+86436062 | MsG0780041090.01.T01:CDS |
| AAAAGGAGATTTGAATGAGG+AGG | 0.578590 | 7:-86436819 | None:intergenic |
| GATCCACCGCCATTACTAGT+TGG | 0.583483 | 7:-86436746 | None:intergenic |
| GTATTGGGGAAGAGGAGCAA+TGG | 0.591759 | 7:+86436583 | MsG0780041090.01.T01:CDS |
| GCCATTTCAGCTTATCTCAG+AGG | 0.596361 | 7:+86436015 | MsG0780041090.01.T01:CDS |
| GCGGTGTGCGAGGGATGTGG+TGG | 0.605687 | 7:+86436649 | MsG0780041090.01.T01:CDS |
| ACACCAACTAGTAATGGCGG+TGG | 0.613692 | 7:+86436743 | MsG0780041090.01.T01:CDS |
| GGTCGTTGCCGGAGAACAGA+CGG | 0.624707 | 7:+86436617 | MsG0780041090.01.T01:CDS |
| CCGCCATTACTAGTTGGTGT+GGG | 0.647594 | 7:-86436740 | None:intergenic |
| AAACCGTTCAAGAAAGCCTG+TGG | 0.653157 | 7:+86436712 | MsG0780041090.01.T01:CDS |
| ATAGGTTGAAGAAGTTCAGG+AGG | 0.653453 | 7:-86436095 | None:intergenic |
| GAATGAAACTTACATGCTGT+AGG | 0.658238 | 7:-86436167 | None:intergenic |
| CCCACACCAACTAGTAATGG+CGG | 0.678913 | 7:+86436740 | MsG0780041090.01.T01:CDS |
| AATGAGGAGGAAGTAACAAG+AGG | 0.678921 | 7:-86436806 | None:intergenic |
| TGTGAAAGACACATGCATCG+TGG | 0.747726 | 7:+86436686 | MsG0780041090.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAAAAATAATGTTACTG+GGG | - | Chr7:86436446-86436465 | None:intergenic | 20.0% |
| !! | CAAGTAAAAATAATGTTACT+GGG | - | Chr7:86436447-86436466 | None:intergenic | 20.0% |
| !! | TTTGATATTTAGATACATGT+TGG | + | Chr7:86436058-86436077 | MsG0780041090.01.T01:CDS | 20.0% |
| !!! | TAAGCAAAAATCAACATTTT+AGG | - | Chr7:86436391-86436410 | None:intergenic | 20.0% |
| !!! | TGATAATGATGAAGAAAATA+AGG | - | Chr7:86436146-86436165 | None:intergenic | 20.0% |
| ! | AGTAAAAATAATGTTACTGG+GGG | - | Chr7:86436445-86436464 | None:intergenic | 25.0% |
| ! | ATATTTAGATACATGTTGGT+TGG | + | Chr7:86436062-86436081 | MsG0780041090.01.T01:CDS | 25.0% |
| ! | CCAAGTAAAAATAATGTTAC+TGG | - | Chr7:86436448-86436467 | None:intergenic | 25.0% |
| ! | TGATTAAAGTGAAGATCAAA+AGG | - | Chr7:86436839-86436858 | None:intergenic | 25.0% |
| ! | TTGATCTTCACTTTAATCAA+CGG | + | Chr7:86436840-86436859 | MsG0780041090.01.T01:CDS | 25.0% |
| ! | TTTCTGTATAATGAGTAACA+GGG | + | Chr7:86436507-86436526 | MsG0780041090.01.T01:intron | 25.0% |
| !! | TTTTTGCTTATAATAGTGAC+CGG | + | Chr7:86436401-86436420 | MsG0780041090.01.T01:intron | 25.0% |
| !! | TTTTTGCTTATAATAGTGAC+CGG | - | Chr7:86436420-86436401 | None:intergenic | 25.0% |
| !!! | AGAAAAGAAAGTGTGTTTTT+TGG | - | Chr7:86436241-86436260 | None:intergenic | 25.0% |
| !!! | CCAGTAACATTATTTTTACT+TGG | + | Chr7:86436445-86436464 | MsG0780041090.01.T01:intron | 25.0% |
| !!! | TGTGTTTTTTGGTTTTGTTT+TGG | - | Chr7:86436230-86436249 | None:intergenic | 25.0% |
| ATCAAAAGGAGATTTGAATG+AGG | - | Chr7:86436825-86436844 | None:intergenic | 30.0% | |
| ATTCTACTTGGTTTCTTACA+CGG | + | Chr7:86436186-86436205 | MsG0780041090.01.T01:intron | 30.0% | |
| CATGTAAGTTTCATTCTACT+TGG | + | Chr7:86436174-86436193 | MsG0780041090.01.T01:intron | 30.0% | |
| CTTTCTGTATAATGAGTAAC+AGG | + | Chr7:86436506-86436525 | MsG0780041090.01.T01:intron | 30.0% | |
| TTTGATTAGTGTTGCAAAGT+GGG | + | Chr7:86436561-86436580 | MsG0780041090.01.T01:intron | 30.0% | |
| ! | ACTTTCTTTTCTTCGTGTTT+TGG | + | Chr7:86436249-86436268 | MsG0780041090.01.T01:intron | 30.0% |
| ! | CTTTCTTTTCTTCGTGTTTT+GGG | + | Chr7:86436250-86436269 | MsG0780041090.01.T01:intron | 30.0% |
| ! | TGAAGAAGACTTTTCTTGAT+AGG | - | Chr7:86436116-86436135 | None:intergenic | 30.0% |
| !! | TTTTGATTAGTGTTGCAAAG+TGG | + | Chr7:86436560-86436579 | MsG0780041090.01.T01:intron | 30.0% |
| !!! | TTGTTTTGGAGTATGTGATA+TGG | - | Chr7:86436216-86436235 | None:intergenic | 30.0% |
| AAAAGGAGATTTGAATGAGG+AGG | - | Chr7:86436822-86436841 | None:intergenic | 35.0% | |
| GAATATTCAATTACACCCTC+CGG | - | Chr7:86436423-86436442 | None:intergenic | 35.0% | |
| GAATGAAACTTACATGCTGT+AGG | - | Chr7:86436170-86436189 | None:intergenic | 35.0% | |
| ! | TTGATAGGTTGAAGAAGTTC+AGG | - | Chr7:86436101-86436120 | None:intergenic | 35.0% |
| AATGAGGAGGAAGTAACAAG+AGG | - | Chr7:86436809-86436828 | None:intergenic | 40.0% | |
| AGTGTTGCAAAGTGGGTATT+GGG | + | Chr7:86436568-86436587 | MsG0780041090.01.T01:intron | 40.0% | |
| TAGTGTTGCAAAGTGGGTAT+TGG | + | Chr7:86436567-86436586 | MsG0780041090.01.T01:intron | 40.0% | |
| TGCTTATAATAGTGACCGGA+GGG | + | Chr7:86436405-86436424 | MsG0780041090.01.T01:intron | 40.0% | |
| TGCTTATAATAGTGACCGGA+GGG | - | Chr7:86436424-86436405 | None:intergenic | 40.0% | |
| TTGCTTATAATAGTGACCGG+AGG | + | Chr7:86436404-86436423 | MsG0780041090.01.T01:intron | 40.0% | |
| TTGCTTATAATAGTGACCGG+AGG | - | Chr7:86436423-86436404 | None:intergenic | 40.0% | |
| ! | ACTTTTTACCGTCTGTTCTC+CGG | - | Chr7:86436628-86436647 | None:intergenic | 40.0% |
| ! | ATAGGTTGAAGAAGTTCAGG+AGG | - | Chr7:86436098-86436117 | None:intergenic | 40.0% |
| AAACCGTTCAAGAAAGCCTG+TGG | + | Chr7:86436712-86436731 | MsG0780041090.01.T01:CDS | 45.0% | |
| CTTCCCACACCAACTAGTAA+TGG | + | Chr7:86436737-86436756 | MsG0780041090.01.T01:CDS | 45.0% | |
| GAACAGACGGTAAAAAGTGG+CGG | + | Chr7:86436630-86436649 | MsG0780041090.01.T01:CDS | 45.0% | |
| GAGGCAAGAACTTGAGTTAC+AGG | + | Chr7:86436034-86436053 | MsG0780041090.01.T01:CDS | 45.0% | |
| GCCATTTCAGCTTATCTCAG+AGG | + | Chr7:86436015-86436034 | MsG0780041090.01.T01:CDS | 45.0% | |
| GGAGAACAGACGGTAAAAAG+TGG | + | Chr7:86436627-86436646 | MsG0780041090.01.T01:CDS | 45.0% | |
| GGGATATGATATACACCCTC+CGG | + | Chr7:86436270-86436289 | MsG0780041090.01.T01:intron | 45.0% | |
| GTTCCACAGGCTTTCTTGAA+CGG | - | Chr7:86436718-86436737 | None:intergenic | 45.0% | |
| TGTGAAAGACACATGCATCG+TGG | + | Chr7:86436686-86436705 | MsG0780041090.01.T01:CDS | 45.0% | |
| ! | GTGTTGCAAAGTGGGTATTG+GGG | + | Chr7:86436569-86436588 | MsG0780041090.01.T01:intron | 45.0% |
| !! | GCCTCTGAGATAAGCTGAAA+TGG | - | Chr7:86436019-86436038 | None:intergenic | 45.0% |
| ACACCAACTAGTAATGGCGG+TGG | + | Chr7:86436743-86436762 | MsG0780041090.01.T01:CDS | 50.0% | |
| CCCACACCAACTAGTAATGG+CGG | + | Chr7:86436740-86436759 | MsG0780041090.01.T01:CDS | 50.0% | |
| GATCCACCGCCATTACTAGT+TGG | - | Chr7:86436749-86436768 | None:intergenic | 50.0% | |
| GTATTGGGGAAGAGGAGCAA+TGG | + | Chr7:86436583-86436602 | MsG0780041090.01.T01:CDS | 50.0% | |
| TAAAAAGTGGCGGTGTGCGA+GGG | + | Chr7:86436640-86436659 | MsG0780041090.01.T01:CDS | 50.0% | |
| ! | ACCGCCATTACTAGTTGGTG+TGG | - | Chr7:86436744-86436763 | None:intergenic | 50.0% |
| ! | CCGCCATTACTAGTTGGTGT+GGG | - | Chr7:86436743-86436762 | None:intergenic | 50.0% |
| !! | CAAAGTGGGTATTGGGGAAG+AGG | + | Chr7:86436575-86436594 | MsG0780041090.01.T01:CDS | 50.0% |
| GTAAAAAGTGGCGGTGTGCG+AGG | + | Chr7:86436639-86436658 | MsG0780041090.01.T01:CDS | 55.0% | |
| GTTGGTGTGGGAAGTTCCAC+AGG | - | Chr7:86436731-86436750 | None:intergenic | 55.0% | |
| ATCCAGAGCCAGGTCGTTGC+CGG | + | Chr7:86436606-86436625 | MsG0780041090.01.T01:CDS | 60.0% | |
| GGAGCAATGGATCCAGAGCC+AGG | + | Chr7:86436596-86436615 | MsG0780041090.01.T01:CDS | 60.0% | |
| GGTCGTTGCCGGAGAACAGA+CGG | + | Chr7:86436617-86436636 | MsG0780041090.01.T01:CDS | 60.0% | |
| TCTGTTCTCCGGCAACGACC+TGG | - | Chr7:86436617-86436636 | None:intergenic | 60.0% | |
| TGTGCGAGGGATGTGGTGGC+TGG | + | Chr7:86436653-86436672 | MsG0780041090.01.T01:CDS | 65.0% | |
| CTCCGGCAACGACCTGGCTC+TGG | - | Chr7:86436611-86436630 | None:intergenic | 70.0% | |
| GCGGTGTGCGAGGGATGTGG+TGG | + | Chr7:86436649-86436668 | MsG0780041090.01.T01:CDS | 70.0% | |
| GTGGCGGTGTGCGAGGGATG+TGG | + | Chr7:86436646-86436665 | MsG0780041090.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 86436002 | 86436865 | 86436002 | ID=MsG0780041090.01;Name=MsG0780041090.01 |
| Chr7 | mRNA | 86436002 | 86436865 | 86436002 | ID=MsG0780041090.01.T01;Parent=MsG0780041090.01;Name=MsG0780041090.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|156 |
| Chr7 | exon | 86436002 | 86436176 | 86436002 | ID=MsG0780041090.01.T01:exon:3128;Parent=MsG0780041090.01.T01 |
| Chr7 | exon | 86436570 | 86436865 | 86436570 | ID=MsG0780041090.01.T01:exon:3129;Parent=MsG0780041090.01.T01 |
| Chr7 | CDS | 86436002 | 86436176 | 86436002 | ID=MsG0780041090.01.T01:cds;Parent=MsG0780041090.01.T01 |
| Chr7 | CDS | 86436570 | 86436865 | 86436570 | ID=MsG0780041090.01.T01:cds;Parent=MsG0780041090.01.T01 |
| Gene Sequence |
| Protein sequence |
