Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041110.01.T01 | YP_009141613.1 | 100 | 96 | 0 | 0 | 1 | 96 | 140 | 235 | 7.18E-63 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041110.01.T01 | Q0ZJ31 | 97.917 | 96 | 2 | 0 | 1 | 96 | 140 | 235 | 4.18E-64 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041110.01.T01 | A0A4P8F193 | 100.000 | 96 | 0 | 0 | 1 | 96 | 140 | 235 | 3.43e-63 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014007.01 | MsG0780041110.01 | 0.805569 | 1.253086e-49 | 6.651656e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041110.01.T01 | MTR_4g051110 | 97.917 | 96 | 2 | 0 | 1 | 96 | 140 | 235 | 2.14e-65 | 197 |
| MsG0780041110.01.T01 | MTR_7g116690 | 90.625 | 96 | 9 | 0 | 1 | 96 | 114 | 209 | 3.01e-59 | 180 |
| MsG0780041110.01.T01 | MTR_0002s0530 | 76.744 | 86 | 20 | 0 | 1 | 86 | 140 | 225 | 1.03e-42 | 145 |
| MsG0780041110.01.T01 | MTR_3g029540 | 95.349 | 43 | 2 | 0 | 32 | 74 | 44 | 86 | 1.23e-23 | 86.3 |
| MsG0780041110.01.T01 | MTR_7g012800 | 97.368 | 38 | 1 | 0 | 59 | 96 | 139 | 176 | 3.18e-18 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041110.01.T01 | ATCG00160 | 91.667 | 96 | 8 | 0 | 1 | 96 | 140 | 235 | 6.03e-60 | 183 |
Find 17 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCATTTGGAAACATATC+TGG | 0.181531 | 7:-86712908 | MsG0780041110.01.T01:CDS |
| CAAGAGACAATTATCTCATT+TGG | 0.294663 | 7:-86712920 | MsG0780041110.01.T01:CDS |
| ATCAACGATTATTACAATAT+CGG | 0.341941 | 7:+86712858 | None:intergenic |
| CTAATTTGTTAAGAATTAAT+CGG | 0.359442 | 7:+86712701 | None:intergenic |
| ATCGAAATATCTGCTAGATC+TGG | 0.363586 | 7:+86712754 | None:intergenic |
| CTTCGAGAATGTATCACTTT+GGG | 0.388824 | 7:-86712814 | MsG0780041110.01.T01:CDS |
| TGATCAGCAAGAAGAATATA+CGG | 0.437056 | 7:-86712839 | MsG0780041110.01.T01:CDS |
| TCTTCGAGAATGTATCACTT+TGG | 0.477646 | 7:-86712815 | MsG0780041110.01.T01:CDS |
| TCAACGATTATTACAATATC+GGG | 0.480431 | 7:+86712859 | None:intergenic |
| GTATCGATTAAACAAATCGT+TGG | 0.491426 | 7:+86712787 | None:intergenic |
| GCTATAGCATCATCATTAGC+TGG | 0.518853 | 7:+86712730 | None:intergenic |
| TCGAAATATCTGCTAGATCT+GGG | 0.525094 | 7:+86712755 | None:intergenic |
| TCTCATTTGGAAACATATCT+GGG | 0.550552 | 7:-86712907 | MsG0780041110.01.T01:CDS |
| GGGTGGCATAAAATATATGA+CGG | 0.585418 | 7:-86712887 | MsG0780041110.01.T01:CDS |
| GGTGGCATAAAATATATGAC+GGG | 0.610359 | 7:-86712886 | MsG0780041110.01.T01:CDS |
| CATTTGGAAACATATCTGGG+TGG | 0.732935 | 7:-86712904 | MsG0780041110.01.T01:CDS |
| GTGGCATAAAATATATGACG+GGG | 0.737363 | 7:-86712885 | MsG0780041110.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTAATTTGTTAAGAATTAAT+CGG | + | Chr7:86712884-86712903 | None:intergenic | 15.0% |
| !! | ATCAACGATTATTACAATAT+CGG | + | Chr7:86712727-86712746 | None:intergenic | 20.0% |
| ! | TCAACGATTATTACAATATC+GGG | + | Chr7:86712726-86712745 | None:intergenic | 25.0% |
| !!! | ATTAGTTTTTGCAATTTGTG+AGG | - | Chr7:86712899-86712918 | MsG0780041110.01.T01:CDS | 25.0% |
| !!! | TTAGTTTTTGCAATTTGTGA+GGG | - | Chr7:86712900-86712919 | MsG0780041110.01.T01:CDS | 25.0% |
| ATCTCATTTGGAAACATATC+TGG | - | Chr7:86712674-86712693 | MsG0780041110.01.T01:CDS | 30.0% | |
| CAAGAGACAATTATCTCATT+TGG | - | Chr7:86712662-86712681 | MsG0780041110.01.T01:CDS | 30.0% | |
| TCTCATTTGGAAACATATCT+GGG | - | Chr7:86712675-86712694 | MsG0780041110.01.T01:CDS | 30.0% | |
| TGATCAGCAAGAAGAATATA+CGG | - | Chr7:86712743-86712762 | MsG0780041110.01.T01:CDS | 30.0% | |
| !! | GTATCGATTAAACAAATCGT+TGG | + | Chr7:86712798-86712817 | None:intergenic | 30.0% |
| ATCGAAATATCTGCTAGATC+TGG | + | Chr7:86712831-86712850 | None:intergenic | 35.0% | |
| CTTCGAGAATGTATCACTTT+GGG | - | Chr7:86712768-86712787 | MsG0780041110.01.T01:CDS | 35.0% | |
| GGGTGGCATAAAATATATGA+CGG | - | Chr7:86712695-86712714 | MsG0780041110.01.T01:CDS | 35.0% | |
| GGTGGCATAAAATATATGAC+GGG | - | Chr7:86712696-86712715 | MsG0780041110.01.T01:CDS | 35.0% | |
| GTGGCATAAAATATATGACG+GGG | - | Chr7:86712697-86712716 | MsG0780041110.01.T01:CDS | 35.0% | |
| TCGAAATATCTGCTAGATCT+GGG | + | Chr7:86712830-86712849 | None:intergenic | 35.0% | |
| TCTTCGAGAATGTATCACTT+TGG | - | Chr7:86712767-86712786 | MsG0780041110.01.T01:CDS | 35.0% | |
| CATTTGGAAACATATCTGGG+TGG | - | Chr7:86712678-86712697 | MsG0780041110.01.T01:CDS | 40.0% | |
| GCTATAGCATCATCATTAGC+TGG | + | Chr7:86712855-86712874 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 86712657 | 86712947 | 86712657 | ID=MsG0780041110.01;Name=MsG0780041110.01 |
| Chr7 | mRNA | 86712657 | 86712947 | 86712657 | ID=MsG0780041110.01.T01;Parent=MsG0780041110.01;Name=MsG0780041110.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|96 |
| Chr7 | exon | 86712657 | 86712947 | 86712657 | ID=MsG0780041110.01.T01:exon:20362;Parent=MsG0780041110.01.T01 |
| Chr7 | CDS | 86712657 | 86712947 | 86712657 | ID=MsG0780041110.01.T01:cds;Parent=MsG0780041110.01.T01 |
| Gene Sequence |
| Protein sequence |
