Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043099.01.T01 | KEH18783.1 | 96.347 | 219 | 8 | 0 | 1 | 219 | 1 | 219 | 2.12E-151 | 429 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043099.01.T01 | Q6DST1 | 27.014 | 211 | 141 | 6 | 10 | 213 | 9 | 213 | 7.05E-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043099.01.T01 | A0A072TPL5 | 96.347 | 219 | 8 | 0 | 1 | 219 | 1 | 219 | 1.01e-151 | 429 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003241.01 | MsG0880043099.01 | 0.810811 | 9.571596e-51 | 5.827412e-48 |
| MsG0180005869.01 | MsG0880043099.01 | 0.804373 | 2.228082e-49 | 1.146912e-46 |
| MsG0280009838.01 | MsG0880043099.01 | 0.803907 | 2.786510e-49 | 1.417334e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043099.01.T01 | MTR_8g028475 | 96.347 | 219 | 8 | 0 | 1 | 219 | 1 | 219 | 2.56e-155 | 429 |
| MsG0880043099.01.T01 | MTR_8g005060 | 38.378 | 185 | 111 | 3 | 36 | 219 | 10 | 192 | 2.97e-35 | 124 |
| MsG0880043099.01.T01 | MTR_8g099035 | 38.009 | 221 | 118 | 7 | 1 | 208 | 1 | 215 | 2.76e-31 | 114 |
| MsG0880043099.01.T01 | MTR_7g074250 | 36.145 | 166 | 101 | 4 | 57 | 219 | 48 | 211 | 3.10e-26 | 101 |
| MsG0880043099.01.T01 | MTR_2g088610 | 28.272 | 191 | 125 | 4 | 32 | 219 | 3 | 184 | 1.75e-20 | 85.5 |
| MsG0880043099.01.T01 | MTR_4g046787 | 31.250 | 192 | 118 | 6 | 32 | 219 | 3 | 184 | 2.18e-16 | 74.3 |
| MsG0880043099.01.T01 | MTR_4g046803 | 30.159 | 189 | 124 | 4 | 32 | 219 | 3 | 184 | 2.58e-15 | 71.6 |
| MsG0880043099.01.T01 | MTR_5g005950 | 28.191 | 188 | 128 | 4 | 33 | 219 | 9 | 190 | 2.15e-14 | 69.3 |
| MsG0880043099.01.T01 | MTR_2g088680 | 26.042 | 192 | 129 | 5 | 32 | 219 | 3 | 185 | 4.53e-13 | 65.5 |
| MsG0880043099.01.T01 | MTR_8g099560 | 25.882 | 170 | 105 | 3 | 58 | 213 | 46 | 208 | 7.14e-13 | 65.5 |
| MsG0880043099.01.T01 | MTR_5g009910 | 26.554 | 177 | 123 | 5 | 41 | 213 | 16 | 189 | 9.72e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043099.01.T01 | AT2G46150 | 41.150 | 226 | 121 | 6 | 1 | 219 | 1 | 221 | 1.60e-51 | 166 |
| MsG0880043099.01.T01 | AT3G54200 | 36.598 | 194 | 121 | 2 | 27 | 219 | 43 | 235 | 6.06e-22 | 90.5 |
| MsG0880043099.01.T01 | AT1G64065 | 27.014 | 211 | 141 | 6 | 10 | 213 | 9 | 213 | 7.19e-19 | 81.6 |
| MsG0880043099.01.T01 | AT3G05975 | 28.866 | 194 | 130 | 6 | 29 | 219 | 1 | 189 | 2.19e-18 | 80.1 |
| MsG0880043099.01.T01 | AT4G23610 | 30.918 | 207 | 127 | 9 | 5 | 199 | 11 | 213 | 1.38e-15 | 73.2 |
| MsG0880043099.01.T01 | AT4G13270 | 20.276 | 217 | 163 | 4 | 3 | 216 | 6 | 215 | 1.65e-11 | 62.0 |
Find 43 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCATTGGTTGTTACTTT+TGG | 0.146018 | 8:+19054451 | None:intergenic |
| TGTACTGTTTCCAAGCTTAA+TGG | 0.225190 | 8:+19054321 | None:intergenic |
| TATTTCAACGAGAGGAATTC+AGG | 0.284868 | 8:-19054041 | MsG0880043099.01.T01:CDS |
| GAATGTTACGATTGATGTTA+TGG | 0.346203 | 8:-19054215 | MsG0880043099.01.T01:CDS |
| GTTTCTAGTCCGAATTTGTT+GGG | 0.347702 | 8:-19054181 | MsG0880043099.01.T01:CDS |
| CTTCACTCTCCCGGGAATTA+CGG | 0.357482 | 8:+19054120 | None:intergenic |
| CTTCTTGGATGGACTCTAAC+TGG | 0.371566 | 8:+19054613 | None:intergenic |
| GGTTTCTAGTCCGAATTTGT+TGG | 0.389579 | 8:-19054182 | MsG0880043099.01.T01:CDS |
| CTTCATCATCACTTCTTGGA+TGG | 0.397355 | 8:+19054602 | None:intergenic |
| TGATGTTATGGCGGACCGCC+TGG | 0.431070 | 8:-19054203 | MsG0880043099.01.T01:CDS |
| GAGATTCAACTCACAAACTT+TGG | 0.432202 | 8:-19054433 | MsG0880043099.01.T01:CDS |
| AACACTTATTCCGTAATTCC+CGG | 0.436075 | 8:-19054130 | MsG0880043099.01.T01:CDS |
| TTCTTCGCCTTCACAAGTCC+TGG | 0.438815 | 8:+19054250 | None:intergenic |
| AAACAAAATCTTCACTCTCC+CGG | 0.456416 | 8:+19054111 | None:intergenic |
| AACAAAATCTTCACTCTCCC+GGG | 0.470476 | 8:+19054112 | None:intergenic |
| AATTGTTATCTTAGCATTCA+CGG | 0.478347 | 8:-19054491 | MsG0880043099.01.T01:CDS |
| AATATAGCATCCATTAAGCT+TGG | 0.490745 | 8:-19054331 | MsG0880043099.01.T01:CDS |
| GTTTGTGAGTTGAATCTCAT+TGG | 0.491391 | 8:+19054438 | None:intergenic |
| TTGATCTTAACTTGAGGTGT+TGG | 0.493235 | 8:+19054391 | None:intergenic |
| GGACATGACATGCACACATA+AGG | 0.506141 | 8:-19054020 | MsG0880043099.01.T01:CDS |
| GGTGTACTACCGCGGTATAA+CGG | 0.525227 | 8:-19054293 | MsG0880043099.01.T01:CDS |
| AGCTAGAGGTCTTCCTTGAT+CGG | 0.526157 | 8:+19054636 | None:intergenic |
| GGATGTGCTTCAAGGGGAGA+TGG | 0.532768 | 8:-19054158 | MsG0880043099.01.T01:CDS |
| TTTCTAGTCCGAATTTGTTG+GGG | 0.549692 | 8:-19054180 | MsG0880043099.01.T01:CDS |
| ACACTTATTCCGTAATTCCC+GGG | 0.571339 | 8:-19054129 | MsG0880043099.01.T01:CDS |
| ACAATGGCAGAGACCGATCA+AGG | 0.579248 | 8:-19054649 | MsG0880043099.01.T01:CDS |
| ACGATTAATATTTCAACGAG+AGG | 0.595890 | 8:-19054049 | MsG0880043099.01.T01:CDS |
| ACAAATTCGGACTAGAAACC+AGG | 0.603060 | 8:+19054185 | None:intergenic |
| TGGACTCTAACTGGAGCTAG+AGG | 0.628727 | 8:+19054622 | None:intergenic |
| AATAGACATGTTCACTACCA+TGG | 0.631088 | 8:+19054363 | None:intergenic |
| TTCTAGTCCGAATTTGTTGG+GGG | 0.636583 | 8:-19054179 | MsG0880043099.01.T01:CDS |
| TTCGCCTTCACAAGTCCTGG+TGG | 0.639016 | 8:+19054253 | None:intergenic |
| AATACCACCAGGACTTGTGA+AGG | 0.652770 | 8:-19054257 | MsG0880043099.01.T01:CDS |
| CTACCGCGGTATAACGGTCG+CGG | 0.655572 | 8:-19054287 | MsG0880043099.01.T01:CDS |
| GTCATGTTGATCTTAACTTG+AGG | 0.659779 | 8:+19054385 | None:intergenic |
| AGTTAAGATCAACATGACCA+TGG | 0.689095 | 8:-19054380 | MsG0880043099.01.T01:CDS |
| TGAAGGCGAAGAAGACAACA+AGG | 0.693934 | 8:-19054240 | MsG0880043099.01.T01:CDS |
| GCTTGGAAACAGTACAACAA+CGG | 0.719713 | 8:-19054314 | MsG0880043099.01.T01:CDS |
| TGTTACGATTGATGTTATGG+CGG | 0.723229 | 8:-19054212 | MsG0880043099.01.T01:CDS |
| GCGGACGCGACAATACCACC+AGG | 0.723867 | 8:-19054268 | MsG0880043099.01.T01:CDS |
| ACAACAACGGTGTACTACCG+CGG | 0.748966 | 8:-19054301 | MsG0880043099.01.T01:CDS |
| CGTCCGCGACCGTTATACCG+CGG | 0.758486 | 8:+19054284 | None:intergenic |
| AATTCGGACTAGAAACCAGG+CGG | 0.769892 | 8:+19054188 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAACTTGATTTTTCTTCTA+CGG | + | Chr8:19054101-19054120 | None:intergenic | 20.0% |
| ! | AATTGTTATCTTAGCATTCA+CGG | - | Chr8:19054164-19054183 | MsG0880043099.01.T01:CDS | 25.0% |
| ! | AGAAGAAAAATCAAGTTATG+TGG | - | Chr8:19054102-19054121 | MsG0880043099.01.T01:CDS | 25.0% |
| AATATAGCATCCATTAAGCT+TGG | - | Chr8:19054324-19054343 | MsG0880043099.01.T01:CDS | 30.0% | |
| ACGATTAATATTTCAACGAG+AGG | - | Chr8:19054606-19054625 | MsG0880043099.01.T01:CDS | 30.0% | |
| GAATGTTACGATTGATGTTA+TGG | - | Chr8:19054440-19054459 | MsG0880043099.01.T01:CDS | 30.0% | |
| ! | TTTTCTTCATCATCACTTCT+TGG | + | Chr8:19054060-19054079 | None:intergenic | 30.0% |
| !!! | ATCTCATTGGTTGTTACTTT+TGG | + | Chr8:19054207-19054226 | None:intergenic | 30.0% |
| AAACAAAATCTTCACTCTCC+CGG | + | Chr8:19054547-19054566 | None:intergenic | 35.0% | |
| AACACTTATTCCGTAATTCC+CGG | - | Chr8:19054525-19054544 | MsG0880043099.01.T01:CDS | 35.0% | |
| AATAGACATGTTCACTACCA+TGG | + | Chr8:19054295-19054314 | None:intergenic | 35.0% | |
| AGTTAAGATCAACATGACCA+TGG | - | Chr8:19054275-19054294 | MsG0880043099.01.T01:CDS | 35.0% | |
| GAGATTCAACTCACAAACTT+TGG | - | Chr8:19054222-19054241 | MsG0880043099.01.T01:CDS | 35.0% | |
| TATTTCAACGAGAGGAATTC+AGG | - | Chr8:19054614-19054633 | MsG0880043099.01.T01:CDS | 35.0% | |
| TGTACTGTTTCCAAGCTTAA+TGG | + | Chr8:19054337-19054356 | None:intergenic | 35.0% | |
| TGTTACGATTGATGTTATGG+CGG | - | Chr8:19054443-19054462 | MsG0880043099.01.T01:CDS | 35.0% | |
| ! | CTTAGCATTCACGGTTTTTA+AGG | - | Chr8:19054173-19054192 | MsG0880043099.01.T01:CDS | 35.0% |
| ! | GATTTTTCTTCTACGGTTCA+TGG | + | Chr8:19054094-19054113 | None:intergenic | 35.0% |
| ! | GTCATGTTGATCTTAACTTG+AGG | + | Chr8:19054273-19054292 | None:intergenic | 35.0% |
| ! | GTTTGTGAGTTGAATCTCAT+TGG | + | Chr8:19054220-19054239 | None:intergenic | 35.0% |
| ! | TTGATCTTAACTTGAGGTGT+TGG | + | Chr8:19054267-19054286 | None:intergenic | 35.0% |
| !! | GTTTCTAGTCCGAATTTGTT+GGG | - | Chr8:19054474-19054493 | MsG0880043099.01.T01:CDS | 35.0% |
| !! | TTTCTAGTCCGAATTTGTTG+GGG | - | Chr8:19054475-19054494 | MsG0880043099.01.T01:CDS | 35.0% |
| AACAAAATCTTCACTCTCCC+GGG | + | Chr8:19054546-19054565 | None:intergenic | 40.0% | |
| ACACTTATTCCGTAATTCCC+GGG | - | Chr8:19054526-19054545 | MsG0880043099.01.T01:CDS | 40.0% | |
| CTTCATCATCACTTCTTGGA+TGG | + | Chr8:19054056-19054075 | None:intergenic | 40.0% | |
| GCTTGGAAACAGTACAACAA+CGG | - | Chr8:19054341-19054360 | MsG0880043099.01.T01:CDS | 40.0% | |
| ! | ACAAATTCGGACTAGAAACC+AGG | + | Chr8:19054473-19054492 | None:intergenic | 40.0% |
| !! | GGTTTCTAGTCCGAATTTGT+TGG | - | Chr8:19054473-19054492 | MsG0880043099.01.T01:CDS | 40.0% |
| !! | TTCTAGTCCGAATTTGTTGG+GGG | - | Chr8:19054476-19054495 | MsG0880043099.01.T01:CDS | 40.0% |
| AATACCACCAGGACTTGTGA+AGG | - | Chr8:19054398-19054417 | MsG0880043099.01.T01:CDS | 45.0% | |
| AGCACATCCCCCAACAAATT+CGG | + | Chr8:19054486-19054505 | None:intergenic | 45.0% | |
| AGCTAGAGGTCTTCCTTGAT+CGG | + | Chr8:19054022-19054041 | None:intergenic | 45.0% | |
| GGACATGACATGCACACATA+AGG | - | Chr8:19054635-19054654 | MsG0880043099.01.T01:CDS | 45.0% | |
| TGAAGGCGAAGAAGACAACA+AGG | - | Chr8:19054415-19054434 | MsG0880043099.01.T01:CDS | 45.0% | |
| ! | AATTCGGACTAGAAACCAGG+CGG | + | Chr8:19054470-19054489 | None:intergenic | 45.0% |
| ! | CTTCTTGGATGGACTCTAAC+TGG | + | Chr8:19054045-19054064 | None:intergenic | 45.0% |
| ACAACAACGGTGTACTACCG+CGG | - | Chr8:19054354-19054373 | MsG0880043099.01.T01:CDS | 50.0% | |
| CTTCACTCTCCCGGGAATTA+CGG | + | Chr8:19054538-19054557 | None:intergenic | 50.0% | |
| GGTGTACTACCGCGGTATAA+CGG | - | Chr8:19054362-19054381 | MsG0880043099.01.T01:CDS | 50.0% | |
| TGGACTCTAACTGGAGCTAG+AGG | + | Chr8:19054036-19054055 | None:intergenic | 50.0% | |
| TGTTGGGGGATGTGCTTCAA+GGG | - | Chr8:19054490-19054509 | MsG0880043099.01.T01:CDS | 50.0% | |
| TTCTTCGCCTTCACAAGTCC+TGG | + | Chr8:19054408-19054427 | None:intergenic | 50.0% | |
| TTGTTGGGGGATGTGCTTCA+AGG | - | Chr8:19054489-19054508 | MsG0880043099.01.T01:CDS | 50.0% | |
| GGATGTGCTTCAAGGGGAGA+TGG | - | Chr8:19054497-19054516 | MsG0880043099.01.T01:CDS | 55.0% | |
| GTTGGGGGATGTGCTTCAAG+GGG | - | Chr8:19054491-19054510 | MsG0880043099.01.T01:CDS | 55.0% | |
| TTCGCCTTCACAAGTCCTGG+TGG | + | Chr8:19054405-19054424 | None:intergenic | 55.0% | |
| CTACCGCGGTATAACGGTCG+CGG | - | Chr8:19054368-19054387 | MsG0880043099.01.T01:CDS | 60.0% | |
| !! | TGATGTTATGGCGGACCGCC+TGG | - | Chr8:19054452-19054471 | MsG0880043099.01.T01:CDS | 60.0% |
| CGTCCGCGACCGTTATACCG+CGG | + | Chr8:19054374-19054393 | None:intergenic | 65.0% | |
| GCGGACGCGACAATACCACC+AGG | - | Chr8:19054387-19054406 | MsG0880043099.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 19054009 | 19054668 | 19054009 | ID=MsG0880043099.01;Name=MsG0880043099.01 |
| Chr8 | mRNA | 19054009 | 19054668 | 19054009 | ID=MsG0880043099.01.T01;Parent=MsG0880043099.01;Name=MsG0880043099.01.T01;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|219 |
| Chr8 | exon | 19054009 | 19054668 | 19054009 | ID=MsG0880043099.01.T01:exon:7218;Parent=MsG0880043099.01.T01 |
| Chr8 | CDS | 19054009 | 19054668 | 19054009 | ID=MsG0880043099.01.T01:cds;Parent=MsG0880043099.01.T01 |
| Gene Sequence |
| Protein sequence |
